輝点計測ソフト 取扱説明書
Contents
1. Area No Area No Total 1 2 3 4 5 Method Method Centroid 2 Gauss Fitting 3 2DGauss Fitting 0 Hand selected Raw Intensity a u Raw Intensity ROI Intensity Center Intensity a u Center Intensity Gauss Fitting 2D Gauss Fitting Intensity Centroid ROI X Position nm X Position nm X2. 6G Count 56 CD G Angstrom Frame 4 4 2 ROI ROI ROI Region Of Interest ROI ROI Region Of Interest 4 4 3 ROI ROI
3. 4 1 4 y gt y y G EE G Count 37 CD G Angstrom 4 1 5 y y
4. G Count 71 GD G Angstrom 5 3 i 5 3 1 G Count G Track G Count G Track 2 7 G Count
5. EE G Count 19 II CD G Angstrom Hand selected Harird sElErtE EE G Count 20 CD G Angstrom 3 2 Histogram Frame Histogram Histogram HistngraITi Sync Currert FotsHEcrrte 3 Mean walue 4550 4443 Y xsp Ysp Raw Irt Intensity x ainE hC Current ntal Courts Ean
6. ROI 6G Count 50 COG Angstrom Gaussian Fitting XY 2D Gaussian Fitting
7. 3 4 1 2 4 1 1 BMP
8. Al Clear 4 3 6 Scale Control pixel frame 6G Count 53 Go G Angstrom 4 3 7 Tracking Control nritrnl ntData mtota Trackng re Threshould Radius Room 3 Pixelrs U Framers NE F lmit 9 Certroid 10 3 2D Gaussian MIh 8F Hand SsBlErtgd 6 Selct al Init Data Tracking Radius FLlimit Min Room Select all Radius
9. Hand selected BG Fixed Gauss Fitting 2DGauss Fitting 6G Count 51 G Angstrom ROI optimization ROI Ref BG BG Back Ground 4 3 5 Count Threshoauld RO SZE Fixells Stop neE iImad Clear hulti IimmadE fT Titmel s 4 ll Clear ROI Size ROI ROI Size n ROI 2n 1 ROI Regio
10. ROI Gaussian Fitting XY 2D Gaussian Fitting Hand selected G Count
11. BMP 6G Count 36 GD G Angstrom 4 1 3 EN G y ROI v ROI H ROI ROI ROI ROI
12. web USB Pentium 32bit National Instruments LabVIEW 8 2 1 SafeNet Sentinel System G Count EI G Count 5 GD G Angstrom 1 3 Web Web http www g angstrom com i
13. TTL 1 5 All S Count Count Area 1 um 2 Intensity Gauss Fitting 2D Gauss Fitting Intensity Centroid ROI 6G Count 58 GD G Angstrom ROI Raw Int
14. 6G Count 33 GD G Angstrom 4 1 2 H E WW ds 2 UL Ts 3 6G Count 34 GD G Angstrom 4 1 2 1 WM O
15. 1 CD ROM CD ROM 2 C amp G Count 8 A G Angstrom EE CD ROM CD ROM G Count WQ T At setup exe setup exe AD amp B gt dotnetfx Files Setupot ms 1dows AF Windowslmst
16. Centroid Gaussian 2D Gaussian Hand selected Select all Centroid Gaussian 2D Gaussian Hand selected EE G Count 55 GD G Angstrom 4 4 4 4 1 PC
17. 6 Centroid Playl 2 Triming Tracking Control Min Copy
18. Centroid ROI Gaussian Fitting XY lt 2D Gaussian Fitting
19. RD a TI g Pa ee Wi 3 CD ROM G Count 6G Count 68 CAA G Angstronm I 2 8 9 4 Files LabVIEW_8 2 1_Runtime_Engine exe LabVIEW Q H dotnetfx axe IExnress Setun Microsoft Dorporatini Setinel System Driver Installer exe nel er stem Driver Installer USB PC 5 2 1
20. mmmammmmmrmmrrYITTITTITTTTTTTITTTTTTT EE G Count 15 DGc angstrom ertroid Hard sglerted auss Frtin le RO nEtimiratinn au HIT Fef HS Centroid Gaussian Fitting 2D Gaussnan Fitting Hand selected Centr0Id a 5 ji Centraid Threshold GauSSian Fitting lt 2D Gaussian Fitting Hand selected 6G Count 16 Co G Angstrom Centroid
21. 1 1000 lt 100 1 100 G Track G Count 72 CD G Angstrom 1 100 6G Count
22. 2n 1 Radius n Radius Radius Radius ROIsize FI limit FI Fluorescence Intensity RS G Count 54 CD G Angstrom Min Min 4 4 Room
23. X Y X SD Y SD Raw Intensity Intensity Single Image TOP Left Bottom Right Counts Mean Extract X Y X SD Y SD Raw Intensity Intensity Single Image 1 5 6G Count 61 GD G Angstrom 4 6 2 Single Image Y xsp sD Rawint mmenswy x FTEHHBEhCW 5D0D0D 1IIHI 15III Riawy Intansity a UL Histogram Single Image
24. GCnunt FGCount G Count EE G Count 43 CD G Angstrom 4 2 080820 gcpx 1 G Count 1 G s 1 New PJ Cpen PJ Sawe PJ Impart F G a 0D H OD 99 45 2 Dl I 4 Da Dp Sp WD 5 About Reset Video IJnfo Method Count Scale Memo Graph Histgram Tracking Trace SW Al Speedy Tracked SW Num SW Trace K ai w512 XH512 36KB 5 145 ourt Rawi rms x 1 50 Tirme S ame Total count 115 Mean Valued 4077 3215 NN 1 5000 10000 Raw Intensity a U 0 O0tCrm 4 5 Top 97 Left 8 146 FE3 Tum22 Battam 353 Riqt 328 0 04659 Counts Pg Mean EE In Frequency 2 1 CT Lm mn ET 1 5000 10000 15000 Raw Intensity a u 4 2 1 E 0 EA
25. B D Ex 9 N IATIFHINI AVI E gcpx gcpx gcpx gcpx AVI AVI AVI AVI NN 6G Count 32 CD G Angstrom G Track1 2
26. Cg Import AVI L 5 gt Wh EE 14 G Count CD G Angstrom 2 1 2 1 l IlNM 2 Ral
27. 4 Speedy 1 e RS G Count 4 COGc Angstrom O CD ROM USB
28. G Track1 2 gtp cfg cfg cfg gcpx G Count 4 1 1 1 MT AVI AVI
29. 9 LM M New PJ Open PJ Save PJ about Reset Video jnfo Method Count Scale Memo Graph Histeram Tracking Trace SWAll Speedy Tracked SWNum SW Trace rs Court Raw Irt i xX qluals a moveavi W512 x H512 36 KB 1 145 ou aw Intensiy sw Tf 3 Tr ean 1 re Hs a ny 50 otset 9 Au 1 1 1 1 Inverse ColorBar 0 50 100 150 Rd HE Time s Frame syne _oure Sei NSR Total Counts 114 Mean Value 249 31923 GaussFiting BG Fixed 5 xs vs 1 I ROl optimization x 2Deeuss Fting Rergo Raw tensty 12 Threshould ROISize Bd 10 5 pt 8 One mage S Fe q 4 AU 2 Loop Mutiimages o 1 1 7 mmee 100 200 300 400 Acew Mean Xpositon nm Extract pvel rame ra nm oo T Tp 458 tem 83 Areatumo Mean 224 sda 1 150 200 250 300 350 MeanXposition nm NUM G 4 D 9 New PJ Open PJ Save PJ About Reset Video Info Method Count Scale Memo Graph Hieteram Tracking Trace 1 145 ICEETETDETTTE FEIPP PP Hg ml OK 7 Pf ottset I nverse colorBar
30. 4 2 3 Graph Histgram Iracking Trace Graph Graph Histogram Histogram Tracking Tracking Contro Trace Trace Analysis EE G Count 45 GD G Angstrom 4 2 4 Vs Dp gt SB SW All Speedy Tracked Sw Num SW Trace SW All ROI Speedy Tracked ROI SW Num SW Trace EE G Count
31. f 1 Tracking Control Min Clear 6G Count 64 GDcGc angstrom Save Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U 6 X Y X Y Position X Position X Position Y Position Y Position X SD Gaussian 2D Gaussian X Gaussian 2
32. USB PC USB USB PC setup exe Sentinel System Driver Installer 7 4 0 exe EE G Count 11 GD G Angstrom 3 G Count GD G Angstrom 3 G Count 3 1 Import 6G Count 13 Go G Angstrom 1 G Count G Count 2 AVI
33. 50 Time S G Count GD G Angstrom i XY Histogram I1 5 AL 2 Histogram 1 5 J Bottom Riciht ES counts 4 Mean 4422 50000 6 g0 4 La In 6G Count 23 D D
34. 5 Q CT MEG wa 1 0 OCR a _ 7 5 7 EE G Count 29 bmp Raw Data in ROI GaussFit in ROI Single Image Extract X Y Position X Position Y Position X SD Y SD Intensity
35. INVERSE 2 COLORBAR 2 VIDEO CONTROL 2 1 EE G Count 48 Co G Angstrom ll 4 3 2 Info Control 24B J 263 49 Raw Data Ih RO USSFItIh R 246 7 wT 263 4 289 sD X
36. Raw Intensity ROI Intensity 4 5 2 Ra Irt Interisty 1 4 150 TTL 3 9 1nn 5H 0 1 50 100 150 TImER Ss Graph Graph Graph 4 5 3 Graph BMP bmp EE G Count 59 CD G Angstrom 4 6 Histogram 4 6 1 ME me 2 SYTIC CUrrertt Total Mean valu094n7 a Y xsD Ysp Raw Irt Intensity 5000 10000 15000 Rawy Intehslty a U FE Un Hattom 353 i 328 0 04553 Courts Ts52 Mean ET
37. 6 Sentinel System Driver Files Sentinel System Driver Installer 7 4 0 exe An A LOD A HD 4 ad el Erle REF etalker LahV1EW BT mtime Er rs me USB PC i G Count 1 6G Count 10 GD G Angstrom 2 G Count 2 3 USB
38. 0 4 1 2 2 PR 3 6G Count 35 CD G Angstrom 4 1 2 3 UM Te
39. Speedy Speedy Speedy Speedyl EE G Count 69 CD G Angstrom 5 2 2 Hand selected 5 2 3 3D ROI Raw Data in ROI 3D GaussFit in ROI 3D
40. S 7 8 jnfo Metl D Histgram Tracking 1 5 Q 00000 s T C E Data No SYNC 304 ecount AN 5 Count1 Ye Ny move avi Spot 1 csy gk sv CSV Oo 3 Info Metl D Histgram Tracking T 1 2 L 1 QU Bg 0
41. Center Intensity a u Center Intensity Center Intensity Gauss Fitting 2D Gauss Fitting Intensity Centroid ROI Mean X position nm X Position X Position nm X Hand Selected ROI EE G Count 41 COG Angstrom Mean Y potision nm Y Position Y Position nm Y Hand Selected ROI Mean X SD nm X SD X SD nm PSF Point Spread Function X SD Centroid HandSelected Mean Y SD nm Y SD Y SD nm PSF Point Spread Function Y SD Centroid H
42. X Y Hand selected GaussFttng BeFxed RO optimization uss Fiting RefBo Threshouid RolSize 5s pas One image ial ll Fm ceer 10 AU Loop Muti images 7 mmes J Acew 1 pkel 1 frame 100000 nm 100000 s LU Count P 0 D HD 9 New PJ Open PJ Save PJ About Reset Video Info Method Count Scale Memo Graph L Histgram Tracking nm moveavi W512 Xx H512 36 KB al Sow Higf otst 0 KI nverse colorBar MI ES bm Centroid Hand selected GaussFiting Brked Rol optimization peassrang RerBo Threshould ROI Size 5 Pb Stop One mage FLm ler AU 10 Mii a ji Loop MuNHmages mmes sd mi N Al Cear 1 pl 100000 nm 1 trame 100000 s G Count B Di 10 00 nm Trace amp Rawrt ensty x 4 3 55 so 45 40 1 i 0 50 100 Time s syne curent Frame 1 Frequency 1 1 1 1 1 100 200 300 400 500 Mean Y potision nm gdrea 1 2 3 4 5 Top 458 Left 63 Area um72 Bottom 228 Riqht 329 0 06145 Courts 58 Mean 30798527 Fi Ja
43. G Count 1 1 G Count 1 Intensity G Count 2 2 1 2
44. G Count GD G Angstrom 1 4 1 1 1 2 1 9 es 4 lt 5 as 6 2 2 1 8 2 2 8 2 3 USB kk 11 3 13 3 1 lt 13 3 2 es 21 3 3 es 25 3 4 es 27 35 28 4 4 1 4 2 4 3 4 4 4 5 4 6 4 7 SSS 32 WE 44 NRC RG 47 i 56 Graph 58 Histoeram 60 Jaeenalywis IE A A A 63 1 2 5 3 USB 68 ec 69 ss 72 6G Count 2 GD G Angstrom 6G Count 3 CD G Angstrom 1
45. ROI X YI 3D ROI 5 2 4 cfg 5 2 5 al a2 a3 a4a5 SDx SDy a6 a8 centroid al a2 a3 a4a5 a6 a8 EE G Count 70 CD G Angstrom 5 2 6
46. 17 COG Angstrom Threshold Thrgshnulgl g Pixelts irm Timels a a 7 ROI Size ROI ROI ROI Size n 5 5 ROI ROI Region Of Interest NN ROI ROI FT limit Centroic One image NE FI I a 4 a REN 84 Fluorescence Intensity EF O Hand selected ROl optimization Loop 2DGauss Fitting Ref_ BG so
47. Histogram Single Image Histogram 4 6 3 Extract Extract EEDO EO Top dr Les 146 FE LT Bottom 353 Right 326 0 045659 Counts 82 ean 4584 26807 FreguUemney LT1 CT Lm HH 1IHIHI 15IHI Riawy Intensity a UL Histogram Extract G Count 62 G Angstrom Histogram Extract Histogram 4 6 4 Histogram BMP Single Image Extract
48. Web Copyright c 2008 G Angstrom All Rights Reserved Microsof WindowsS Microsoft Corporation Pentium Intel Corporation National Instruments NI LabVIEW Nationallnstruments Corporation Sentinel SafeNet 6G Count 6 GD G Angstrom 6G Count 7 COGc Angstrom O 2 2 1 1 2 2 2 G Count
49. bmp 4 7 Trace Analysis N ace Analysis Total trace number is 29 0 2 2 174 hositioh bixels CE CE BE 297 174 296 173 295 293 cD Fd x Fosition YT Position 5 5D 30 20 gn Tritning gn Intensity Data No Data No 6G Count 63 CD G Angstrom yy SYNC Data No Frame
50. 73 GD G Angstrom C 2008 G Angstrom 2 1 5 2 0 6G Count 74
51. y Um y Video Contro Info Controlj Method Control Count Scale Control Memo EE G Count 38 GD G Angstrom 4 1 6 7 TE 9 TI 9 4TF Single Image Extract Tracking Controlj Trace Analysis 4 17 D
52. raW A aQ Es Te so 0 GtHO ab Re 9 CES Qut set Viden jmfo Method C 1 4 ove avi 512 Xx H512 36 KB 1 AV ay R a y GTrack1 2A G 9 nn Save PJ Save G Count 30 CD Aingstro om G CO unt 31 C G Angstrom 4 4 1 1 Orn i 0 cho ie 8 All AV 0 ct AV Q AV R GTrack1 2 Gj
53. Hand Selected ROI Y Position nm Y Position nm Y Hand Selected ROI X SD nm 6G Count 40 GD G Angstrom X SD nm PSF Point Spread Function X SD Centroid HandSelected Y SD nm Y SD nm PSF Point Spread Function Y SD Centroid HandSelected CSV ime 1 Area No Area No Total 1 2 3 4 5 Count Raw Intensity a u Raw Intensity Raw Intensity ROI Intensity
54. 46 GD G Angstrom 4 3 Control 4 3 1 Video Control IhwfSB I FH T YW GAIN OFFSET INVERSE COLORBAR VIDEO CONTROL Slow High GAIN OFFSET RS G Count 47 CD G Angstrom
55. Raw Data in ROI ROI GaussFit in ROI Gaussian Fitting 2DGaussian Fitting 4 4 4 BMP bmp EE G Count 57 GD G Angstrom 4 5 Graph 4 5 1 Ra Irt Iterstty 4 UT a 100 150 TIPmETS Count Raw Intensity Intensity X Y X SD Y SD
56. 2 6G Count 39 CD G Angstrom BMP
57. GE New FI Open PJ Sawe FJ Import About New PJ gcpx Open PJ gcpx Save PJ gcpx Import About G Count 6G Count 44 CD G Angstrom 4 2 2 H 0 E S9 w 9 Reset wideo Info Niethod Count Scale Memo Reset Video Video Controlj Info Info Control Method Method Control Count Count 6 Scale Scale Control Memo Memo
58. EL E Clear Mutti images L O Loop 7 Time s I G Count 18 CD G Angstrom 999 1 0ne Image one image One Image 2 ui imadgs Multi images Multi images 5 RE AII frames 3
59. GaussFit in ROI 3D 6G Count 49 CD G Angstrom XxX Y X Y D GaussFit in ROI 3D ROI 4 3 3 Info Control 3D Raw Data in ROI 3D GaussFit in ROI 3D bmp 4 3 4 Method Control DHarnd selected Gauss Fitting Fixgd w RDptirmization TDzpeauss FIh Ref Centroid Gauss Fitting 2DGauss Fitting Hand selected Gauss Fitting 2DGauss Fitting Centroid
60. New PJ Open PJ Save PJ can too g em lt mverse colorBar x Y 1 Centroid Gauss Fiting BG Fived ROIoptimization 2DGauss Fiting Hand selected G 9 D Video jfo Method Count Scale Memo Graph Histgram Tracking Trace RerBe Threshouid RoISize ia 5 pbek One image lg 9 Frimt lear 10 Au 7 Timets _Loop Muti images ai mu a Al Clear toa mama Threshould Radius Room 5 ppeks 0 Framets cenog au 1 Gaussian mal 2D Gaussian Min Hand selected 5 Framets Selctal a 3 SW All Speedy Tracked SWNum SWTrace Count Rawint ntensty X 4 mL 1o s 1 8 4 sd ALL 0 1 0 5 10 Time Frame 1 Fr swe _curent Total Counts 113 Mean Value 4109 3827 xsp Ysp Rawit mensty x Frequency Wp 1 1 5000 10000 15000 Raw Intensity 3 u Extract 1 2 3 4 5 Top Let Area um2 Botom Reht move avi W512 xX H 512 36 KI untitled 1 G Count
61. 172 spty 176 save X Y ROI X CX Y Y Raw Data in ROI 3D GaussFit in ROI 3D X Y SD X SD Y save Raw Data in ROI 3D ROI X YI 3D GaussFit in ROI 3D ROI XYI 3D GaussFit in ROI 3D ROI X Y SDQ SD Y ROI X X Y Y X SD X Y SD Y ROI save Raw Data in ROI 3D
62. Freduehcy ah eh LT Cn 50D0D0 10000 15000 Raw Intensity a 0 Frame Sync 6G Count 60 CD G Angstrom Frame Current VY Extract Total Counts Frame 6 Mean Value Frame X Y X SD Y SD Raw Intensity Intensity Single Image Single Image Frame
63. Counts Mean Frequency Raw Intensity 2 u NUM Tracking Control Tracking Tracking Control Track ing D TD amp D 7 Tracking Control Tracking 9 New PJ Open PJ Save PJ About Reset Video jmfo Method Count Scals Memo L W Graph Histeram Tracking Trace SWAll Speedy Tracked SWNum SW Trace fm CEE TT eon mwm nx KS em 1m 5 o ko vp 3 p 5 4 otst 0 3 J 4 ad nverse ColrBar rm 0 i 1 0 5 10 3 Time Frame 1 Sync Current Total Counts 113 MeanValue 4103 3527 ROloptimization xsp Ref Be Rawm mensty x Threshouid Rol Size 6 5 Pe One
64. a 4 Graph Histgram Tracking Trace SWAll Speet Sow Hgh Gan 100 ED 1 ad Inverse colorBar ER EO ce meeswas Gauss Fitting BG Fixed Roloptimization GeussFting Retpo Threshoud ROI Size SR 5 oneanage PH dear 1 Loop Mutiimages 7 mm wd a ud Al Clear 1 pgl 1 fame 100000 nm 1oooo mpaa ems Tmreshoug Radius Room 5 pixele Frame s aj ad EE FL Certroid 10 Au I Caussian 20 Gaussian Mn FZ Hand selected 1 move avi W512 Xx H512 36 KB untitled 1 G Count New PJ Open PJ Save PJ Gan 1 yo ud mi a wa SCZ ial Inverse colorBar EYE ROl optimization 2pewssrane FP Rat Bo Threshouid ROI Size es 5 ppats a da FE PH ee 10 Au Loop Muti images Time Acew 1 pl 1 frame 100000 nm 100000 s Int Data Tracking Threshouid ts Re 5 pele 0 Framets 4 sd I Centroid AU I caussian 2DGaussian Mn Hand selected Certroid Hand selected Gauss Fiting BGFixed 70Y D 9 240 235 230 so5 aa ES 0 10
65. x 1 1 5000 10000 15000 Raw Intensity 3 u a ea 458 Let 62 Areaum2 Botln 211 Ram 334 006746 cts a Mean 30017145 Frequency 1 1 1 5000 10000 15000 Raw Intensi a u Data No Trace Analysis Data No O ROI Trace Analysis 27 CD G Angstrom 3 5 bmp G Count gcpx lt A 9
66. X SD pixels Y SD pixels Intensity A U Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U 1 Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U 1 4 7 2 Pnsitinh issi 293 174 x position hixels Trace Analysis Trace Analysis 47 3 BMP E
67. Yalue 4550 4443 Ys Histogram Total Counts Raw Irt Intensity Frame l1 an ED roa canis vemnvave aoa Y xs sp Histogram Mean Valuel Rawnt mtensty x 6G Count 21 G Angstrom Frame 1 I Sync _ Cure Single Image Total Counts 9 hiean walue 4550 4443 xsp Histogram Single Image Single Image Graph COUrt Fawy Irt Interisity hi k mll 1II Tirmg ts Graph Graph Graph Graph float
68. image dd Flim Clear 10 AU Loop 7 Timets 1 picel 100000 nm a Mutiimages AlClear 1 frame 100000 s 1 1 5000 10000 15000 Raw Intensity a u Extract lt lt 5 Top Let Area um2 no re Botom Roht counts Mean Threshould Radius Room RR 5 Peis 0 Frame s Rl centroid Frequency 2D Gaussian Min Hand selected 5 Frame Raw Intensity 3 0 WF NUM G Count 25 G Ang BD untitled 1 G Count amp D Gain 100 ems 4 mwerse colorBar EVEN 9 New PJ Open PJ Save PJ About Reset Video jnfo Method Count Scale Memo Ph Graph Histeram Ea Sow Hgh SO by Centroid Hand selected Gauss Fiting Be Ficed ROloptimzaton 2Desuss Fiting mato Threshouid ROI Size 2 5 Fyete One image Flimt Cear 10 AU i Loop Mutiimages 7 Timets fd a mi Al Clear moa emg Threshould Radhs Room 5 Paks 0 Frame s EEEI 4 FL J Centroid 10 au I Gaussian sal a 2D Gaussian Min I Hand selected 5
69. 0000 s amp C b CE Data No SYNC 304 6G Count 28 CD G Angstrom IDataNo r Trace Analysis Save Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD Dixels Intensity A U nm kW 1 D 0 Tece L ES o
70. 0200 X Position mM Ga 0 100200 YPosition Tracked SWNum SW Trace 10 i 240 238 4 _236 AL 3 1 234 lt 0 5 10 gt Time s 232 20 Frame 1 Fsme cmw Total Counts 113 Mean Yalue 4103 3827 Sg Y xs vs 226 Rawrt mensty x 224 1 1 1 284 286 288 290 Xpositon pixels Tming copy cear save Frequency Rawint intensty X ii 1 1 5000 10000 15000 Raw Intensity 3 U Frequency Graph Histgram Tracking Trace SWAll Speedy Tracked 5 Frame s Sea G Count B pal pano 9 240 239 236 Position pixels 224 1 284 286 288 Xposition pixels SYNc Frame certo Play 1 34 gt 4 226 YPosition 1 0 100 200 Intensity 250 soo Extract ES IE2 IE 66 os Too 458 Let 62 Aaeacm YSD Intensity Botom 211 Rimt 334 0 06746 counts 7 Mean 400171436 15 1 1 5000 10000 Raw Intensi 3 u 1 15000 NUM SW Num SWTace Court Rewm msy x lt 0 8 8 4 ALL 9 I 1 0 5 10 Time Frame 1 sme crew yl Total Corts 113 Menvake 6555 y xsp Ys Rewh mesy
71. 1 1 1 1 1 200 250 300 350 400 450 Mean Y potision nm NUM amp SW All Spesdy Tracked SW Num SWTrace Rawrt mensty x TT WP 200 1 0 50 100 150 Time syne current Total Counts 114 Mean Yalue 52 56424 Y xsp Ysp Rawt mensty x Frame 1 40 Frequency In 1 T 1 1 1 50 100 150 200 250 Center Intensitya u Tp 103 Let 181 Area cum2 Mean 58 85297 50 100 150 200 250 Center Intansity a u NUM 8 Histogram Single Image Extract Extract Graph Histogram 1 5 Graph 1 5 Graph 1 5 24 G Angstrom 3 3 E
72. D Gaussian X SD pixels 1 Y SD Gaussian 2D Gaussian Y Gaussian 2D Gaussian Y SD pixels 1 Intensity 4 7 1 Trace Analysis Trace nalysis Tntal trace number is 30 baaNo 40 lt le SrhMC Frame hw Centroid FIay i Trace Analysis a 243 MS 5 W 242 EE G Count 65 CD G Angstrom Data No Frame No X Position pixels Y Position pixels
73. E G Count 66 G Angstrom bmp 4 7 4 297 295 eg5 g 2 i eg WX PFoasition TFPFoasition 2 5 1 5 Trace Analysis 6G Count 67 G Angstrom 5 FAQ 5 1 USB 5 1 1 USB USB G Track 1 CD ROM USB 0 2
74. Framets Selctal a Ft G Count SH racking Trace Deta No 1 2 TL 1 213 2125 211 210 5 M SWAll Speedy Trackld SWNum SW Trace cour Rawint ntensty X 0 sYNc Frame IZ Certroid 1 s a 8 4 ALL 9 1 1 0 5 10 Time Frame 1 sm _curent Total Courts 113 Mean Value 4109 36275 kd xsp YSD Rewmt mensty x 20 210 275 1 1 276 277 Xposition plxels Frequency 1 1 1 1 5000 10000 150nn Raw Intensity 3 U 60 Extract 40 1 2 3 4 5 1 5 0 20 YPosition Top 458 Let 62 Area Cum2 Intensity Batom 211 Riaht 334 006746 couts 70 Mean 4001 71436 15 10 5 1 1 1 5000 10000 15000 Raw Intensity 3 u NUM Trace Trace Analysis Trace O TOIT EE nalysis 26 G Angstrom 3 4 Q New PJ Open PJ Save PJ About Video 9 jo D Method Count Scale Memo pp
75. allerd 1 4 i SgtuD BXB Setup GCount USB PC G Count Microsoft NET Framework Version 2 0 PC Files dotnetfx exe Microsoft NET Framework Version 2 0 LabVIEW Runtime Engine 6G Count Go G Angstrom Files LabVIEW 8 2 1_Runtime_Engine exe LabVIEW T H gt Sehtinel System Driwer jnstallsr 4Dexe Sehitinel System Driver Installer USB PC
76. andSelected ROI 4 1 7 1 UI 4 Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U 1 6G Count 42 GD G Angstrom Data No Frame No X Position pixels Y Position pixels X SD pixels Y SD pixels Intensity A U 1 4 1 8
77. n Of Interest ROI ROI FI limit FI Fluorescence Intensity Loop Stop 6G Count 52 CD G Angstrom One image 6 Clear Multi images All frames
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