京都府内で発生した腸管出血性大腸菌感染症事例の IS
Contents
1. IS printing system 0157 IS printing system key words Enterohemorrhagic Escherichia co77O157 Epidemiological analysis pulsed field gel electrophoresis IS printing system 1996 0157 10 3 4 2009 8 9 A 0157 H7 1 2 O0157 VT1 VT2 8 32. PFGE IS printing system 6 PFGE 24 TIS printing system DNA 4 PCR PFGE ISprinting system 0157 ISprinting system 55 2010 1 2010 lt gt
3. pp155 157 6 1997 PFGE Tb63 012 7 2009 O157 H7 28 37 40 8 2008 O157 H7 PFGE IS printing system 41 29 31 9 2009 0157 H7 PFGE IS printing system 59 67 71 10 1987 3 B2 B13
4. DNA 1 SMac OXIOD 36C 24 3 NuSieve GTG Ararose Lonza Seakem GTG Agarose Lonza 2 1 1 1 0 18 3 3 4 0157 3 5 2
5. IS printing system 1st set primer mix 6 110100111100111101 2nd set primer mix 6 011100100111101111 6 A 2 5 1
6. 0157VT VT2 10 O157 VT1 VT2 0157 PFGE 9 PFGE 2 IS printing system 2 A 8 28 8 30 9 9 9 9 11 9 13 9 14 9 16
7. 2010 5 31 152 156 2 2010 A 0157 31 156 157 3 2003 PFGE pp95 104 15 4 Tadasuke Ooka Jun Terajima Masahiro Kusumoto Atsushi lguchi Ken Kurokawa Yoshitoshi Ogura Md Asadulghani Keisuke Nakayama Kazunori Murase Makoto Ohnishi Sunao Iyoda Haruo Watanabe and Tetsuya Hayashil 2009 Development of a Multplex PCR Based Rapid Typing Method for Enterohemorrhagic Escherichia coli O157 Strains Journal of Microbiology 2888 47 5 2004
8. 55 2010 IS printing system Examination of the Epidemiological Analysis of Escherichia co77 O157 Using IS printing System and Pulsed field Gel Electrophoresis Norio ASAI Satoko NAKAJIMA Sugio YANASE Ariko MITANI Emi KAWAMURA Shinya KOJIMA 2009 9 21 0157 2009 8 9 0157 0157 IS printing system
9. IS printing system A 3 3 IS printing system 1 8 9 A 2 PFGE Tryptic Soy Broth Becton Dickinson 36C 1 PFGE X gl 30 units sample BInI 40 units sample 37C 2 55 2010 1 O157 H7 No1 No2
10. 8 17 26 8 16 9 2 2 2009 9 21 0157 O157 VT1 VT2 A 22 9 1 Kyoto Prefectural Yamashiro Kita Public Health Center Kyoto Prefectural Yamashiro Minami Public Health Center O157 PFGE
11. 9 20 0O157 VT1 VT2 9 26 9 27 3 4 O157 VT1 VT2 0157 VT1 VT2 0157 VT1 VT2 55 2010 X ag1 BInI Marker Nol No2 No3 No4 No5 No6 Marker Nol No2 No3 No4 No5 No6 Marker a ke gt 1 O157 XbaI BInI 1 Marker Salmonella Braenderup H9812 3 IS printing system 1 stset 1 2 7 8 9 10 11 12 13 14 15 16 17 18 No1 No2 No3 No4 No5 No6 OO O 6 2 Nd a
12. No3 No4 No5 No6 BioRad CHEFF DRII BioRad TBE Buffer 0 5M Tris 0 45M Boric acid 0 005M EDTA 6 V cm 2 0 54 2 18 0 12 5C A A A 8 9 16 42 9 23 11 9 23 2 2 Salmonella Braenderup H9812 Xbal 3 IS printing system IS printing system version2 TOYOBO
13. d 2 nd set 3 1 2 3 4 Pa 8 11 12 13 14 15 16 17 18 O1 No1 No2 No3 No4 No5 No6 6 oO 1 1 0 0157 9 PFGE ISprinting system 9 PFGE IS printing system PFGE IS printing system A No1 No3 3 No4 No6 3 2g1 zlI PFGE 1 IS printing system 3 XbaI BlInIl No1 No6 6
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