ReadiDrop Cell Viability Assay 取扱説明書 - Bio-Rad
Contents
1. ReadiDrop Cell Viability Assay 135 1101 ReadiDrop Propidium Iodide 3ml x 3 135 1102 ReadiDrop 7 AAD 3ml x 3 ReadiDrop cell Viability Assays 1 ReadiDrop ReadiDrop PI 7 AAD 1 2 ReadiDrop DNA RNA RI Add 1 2 drops to 2 x 108 cells ml 2 Mix cells with ReadiDrop Assay 9 Sort and analyze using the S3 Cell S2. 4 0331 Fax 03 6404 0334 MNL1451101 M10677 1506B
3. orter or any flow cytometer or cell sorter 1 3 ReadiDrop Cell Viability Assay 3ml x 3 S3 488nm S3 488 561nm S3 488 640nm 135 1101 ReadiDrop Propidium Ilodide PI FL3 615 25nm FL3 615 25nm FL2 593 40nm 135 1102 ReadiDrop 7 AAD FL4 655LP FL4 655LP FL3 670 30nm 400 500 600 700 400 500 600 700 800 Wavelength nm Wavelength nm 9 9 2 ReadiDrop PI 3 ReadiDrop 7 AAD 347 426 Dead Dead cells cells 260 320 3 174 3 213 87 107 0 10 10 10 10 109 10 102 103 FL3 area log FL4 area log 4 ReadiDrop Cell Viability Assay Jurkat 1 ReadiDrop PI A 7 AAD B ML Visit us at http www bio rad com 140 0002 Tel 03 6361 7000 Fax 03 5463 8480 2 2 24 Mail life_ps_jp bio rad com Tel 03 640
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