FPLC（GP-250 Plus）簡易マニュアル
Contents
1. 4 a 6 10 18 mm 18 28 mm 5 soe cahbration mode Ei LCC501Plus Cali ml 1 O Store return2. 25 4 1 2 2 Panpantpremter 6 3 A Column B A 4 NG Injection Port cnnnectinns 4 1 3 6 fraction N N 4 1 4 P 5OO 4 2 RUN METHOD
3. UV RUN END LCC 501 Plus 14 I0Y I271 2 1 FPLC 2O A B Wash 3 IManual Etep Forwordl 4 Wash AB OO 11 1 do store 5 35 Wash AB OO LOAD 1
4. U V UV 1 FRAC 200 100 LGAGNOC JO 2 MANS ELECTOR Yokage es Event mark SN 1O mV O mV HI WPE FtiCTIOM COLLECTK 17 16 13 12 1 1 3 Pharmacia Pharmacia LKB Pump P 500 OO de PULSE FLOW RATE coMF ll Pharmacia Pharmacia LKB Pump P 500 LSE LVENTFOVER
5. 27 1 GP 250 LS METHOD TINEA VODL ai RUN REMOTE HOLD GRADIENT UN nn 9 VOLUNE PALEE rT FF w slsrm pause reset paper Feed larm reset P 5OO Limit SET Limit alarm reset 28 2 end end 1 RUN METHOD O 2 Conc B O
6. po store IV 7 V 7 INJECT 2 26 5 RESOURCE S RESOURCE S 1ml BSA pl 4 7 4 9 A pl 8 5 A pl 90 C pl 10 1 pl 110 11 4 2 5 mg 10 ml Buffer A 1 ml Buffer A 50 mM MES Buffer CpH 6 O Buffer B 50 mM MES Buffe 1 M NaCl pH 6 O 2 ml min 0O 5 AUF 1 0 cm ml MES Buffer pH 6O BSA BSA RESOURCE S Buffer B
7. O 9 do store 0 1 2 3 4 5 6 7 8 9 B1 1 p26 p23 DELETE METHOD OC9 do store 32 COPY FROM TO COPY FROM TO X Y X Y XY O lt 9 ldo store 1 BANK COPY FROM
8. P 5OO P 5OO 110 1 cm 200 11 1 4 Manual Manual A CONC B O O Step Foward ML MIN OO 5 pgo store A 5 ml min B amp Step backward 2 CONC B O 0 100 ido store
9. do store Min O ML 1 DL 2 X ML DL P 5OO ML Volume PUMP CAL AB AB O 1 ml P 500 110 REC CAL 1 cm 200 236 CALIBRATE PUMP A A B P 1 CLEAR MEMORY Calibration Method File 441 do store RUN METHOD MI VOLUME
10. 1 Load 1 hold gradient AB pause amp cont 3 method File cal evaluate manual p32 p38 p47 p41 4 insert delete change p24 xit 29 5 step forward amp step backward do store 30 2 Method File Method File Me
11. 6O do store P POL 0 1 1 0 0 O O FPLC 10 9 5 6 7 PPOL OOOO000 8 9 O 4 8 9 7 1 2 O 4 P POL O 0 0 0 0 0 0 XY X O 4 8 9 Y 1 O 1 O do store 3 1 8 1 PPOL OOO 1 0 1 0O 37 LCC5O1 Plus Start Stop PSV 5O 10
12. O 9 do store 0O 1 2 3 4 5 6 7 8 9 B1 1 p23 p20 19 3 2 3 a OOO _ CONC B 0 glo store Step Forward
13. IManual CONC B Oo 50 do store AB ML MIN OO do store 1 9 Step Forword 1 PT OOOOOOOOO OO 8 1 do store LOAD 1 INJECTION 2 END 15 2 2 P 5OO lt 4 Manual EBtep Forword X 1 ML MIN O00 DO5 do store Step Backwordd X 1 ML MIN OO do store X
14. 20O CONC B OO Delete 20O CONC B OO Ho stpre 5OO CONC B OO exit change 1 OOO CONC B OO 200 CONC B 5OO OOO CONC B OO lchange OOO CONC B OO 2 0 do stpre 2 00 CONC B OO 50 0 do stpre 2 00 CONC B 50 0 exit 24 4 4 1 4 1 1 UV UV 1 Pharmacia PharmaciaLKB Control Unit UW 1 a UV Short UV 1 2
15. 3 1 Emlumn Column Pump Column Pump FE IN OUT INJECT PSV 5O FRAC1OO NC NO 1 2 UV Director amp 1 3 1 3 1 P 5OO RUN ON Pharmacia macia LKB Pump P 500 rh OW Fi SOLVENT FOVER SET LIMIT PRESSUFE TE CHANGE e ee 1 1 3 2 UV UV 1 AOuE os
16. tep backward go store b LCC 501Plus 2 CONC B CONC B 3O B 1 5 24 ml B
17. METHOD1 BANK1 PATH 1 1 c2 3 METHOD2 BANK3 UBE NO RUN 41
18. WASH AB 39 AB AB P 5OO 3 35 ml WASH AB XY X A 1 O ON 1 OFF OO Y B 1 O ON 1 OFF O do store WASH RR A TN CA WASH PAUSE FEED TUBE 1 1 SYNC A B 1 15 ml SYNC AB XY X A 1 O ON 1 OFF
19. B ML DL MIN AB OONC B 50 1 O AB O5 ml dl 1 0 ml dh min CM MIN 1 PT OOOOOOOOOO ON OFF FPLC 10 O 9 5 6 7 O 4 8 9 7 PT OOOOOOOOOOOO X Y X O 9 Y ON OFF 1 O ON 1 OFF O do store 5 1 PT OOOOO 1 OO0O0 56 7 ON 5 1 PAUSE 6 1 7 1 FRAC 2OO
20. 3 CV 10 CV C3 5 CV 10 20 CV 18 3 2 3 2 1 Calibration Cal MinO ML 1 DL2 ML 1 do store 3 2 2 Method File Method File 0O 1 2 3 4 5 6 7 8 9 Bf1 B No 1 5 do store FPLC 5 B1 5 10 0C9 50 2 7 O 1 3 4 56 89 B1 lt Step Foward 2 PROGRAM METHOD
21. BANK COPY FROM AB A A B O lt 9 do store IBANK_COPY__TO BANK COPY TO CD C D CD O lt 9 ldo store 1 33 CONC B ML DL MIN CM MIN ML DL PORT SET p4 1 4 Manual 34 35 3 Callbratlon Calibratton Cal Calibration Btep Forward Step backward
22. 3 5 END 2 3 A B WASH 3 Manual Manual Step Forword 4 Wash AB OO 1 1 po store Wash AB OO gt z WASH 3 WASH 1 LOAD Ch O6 Ch O7 P 5OO Limit SET Limit Limit
23. B 5 mmin B Step Foward 1 2 lt CM ML OO 5 po store lt Step backward X 1 CM ML 5 0 O glo store PT OOOOOOOOO_ OO 6 1 go store PSV 5O
24. glarm reset 16 2 4 Step Backwordl 3 ML MIN OO X do store END X 3 5 UV 17 3 1 ml CV
25. 26 4 1 4 26 4 2 26 5 27 1 LCC 501 Plus 28 2 Method File 31 3 Calibration 36 4 Manual S98 5 Evaluation 41 FPLCO 1 1 1 1 1 REMOTE PUMP A PUMP 19 6OO5 O2 MIXER GP 250 Plus FESETY IGROSIS WWTCH TYRLYE DIER RECOSDER eSCOTA 24YRC OUTPORTS AXIO N5 MAX 300 ma AI LN3IGVYD 12889 PUMP A FUMP E GE 4 567 10 1 1213 1415 13 17 151920 212223 2425262728 FRACTIONCOLLECTUK P 5OO 1 1 2 REC101 111 102 112 FRAC 10O 200 B
26. 6 64 1 3 4 FRAC10O 200 Ta Phamacia LKB Frac 200 1 2 3 4 5 CE Feuea eo 4 howk hbed Jher K CE mode fowrste Bhindsta ue Ea drsbon ec feed 5 8 9 9 tote wt SPe day Ee da eu ON 1 drop min 2 sore cahbrate flow rate N P 500 lt 2 3 HH 5mm
27. go store Step backward 1 ep lt PROGRAM METHOD co store Step Foward Step backward c LCC 501 Plus insert delete change st 1 O ee NA 5 00 CONC B OO OOO CONC B 00 Insert OOO CONC B OO 2 0 Ho stpre 2 0O CONC B OO Step Forward 2 00 VALVE POS OO VALVE POS 1 1 go stpre 2 00 VALVE POS 1 1 exit 23 eete 1 OOO CONC BOO 200 CONC B OO _ 5OO CONC B OO
28. O 9 SCOUTDORTESc 5 6 7 max 200 ma Na a PPOLU i eri a PSV 5O O 6 PSV 10O 5 1 18 2 19 3 2 PSV 5O 4 3 4 PSV 5O 8 21 9 22 Start Stop 38 4 Manual Manual Manual Step Forward Step backvward do store CONC B AB
29. ON OFF AU rnans Pharmacls LkE Ctleal Urd Uy ty Ph fn ER rt inl Fr Tn mwlF Ir mmly COPIKAI WM 1 3 3 REC102 1 12 OS 9 ON int ext ext Rec OFF ON ON Chart Speed 2Omm seo 1V B 1OmV UV 1 6 1 Zero Suppr O 6 2 Cal Var Cal 6 3 Pen up down down 6 4 Zero Zero 10 O
30. lt Step backward X 1 PT OOOOO1OOO_ OO 6O po store END 13 UV UV height area UV UV 1 2 AE
31. 1 1 Store return 1 2 Store return 1 3 Store return 1 4 Store return 11 5 Store return FRAC2OO UV 08 mm 502 651 m 1 2 mm 1 13 mm delay N 6 N Store return 9 N Store return O N Store return End at N Store return 1 3 5 GP 250 Plus ROW METHOD TIMEWOL TE Bm RUN REMOTE HOLD GRADIENT UN OD 0 YOLUME PALEE F
32. B CONC B O 4 14 16 21 ml 21 c 21 0 ee CFF 7O 4 14 16 21 mn ml OO ON ON p22 Insert p24 OOO CONC B OO OOO _ ML MN 1 00 OOO CM NIN O5 OOO PORT SET 6 1 400 CONC B OO 140 CONC B 500 140 CONC B 100 0 16OO CONC B 1000 16OO PORT SET 6O 16OO CONC B OO 21 OO CONC B OO 22 3 2 4 a Method File lt Step Foward 2 LIST METHOD
33. OO CONC B 30 00 36 00 CONC B 30 00 30 ml 20 1ml 1ml 0O BB 0 BB 3 3 3O0 100 B 3 2 amp O BB 2 CONC B CONC B 0 B CONC B CONC B CONC B CONC B CONC B CONC B 0 ml CONC B 0 O1 4 7 10 12 30 1ml 1ml O BB O B 3 O 100 10 lt 100 B 2 0O BB 2 B CONC B CONC B CONC B 50 CONC B CONC B CONC
34. FPLC GP250 Plus FPLC GP250 Plus 1 FPLC 1 1 1 1 1 1 1 1 1 1 2 2 1 1 3 3 1 2 4 1 3 4 1 3 1 4 1 3 2 UV 5 1 3 3 6 1 3 4 7 1 3 5 10 1 4 12 2 15 2 1 15 2 2 16 2 3 16 2 4 17 3 18 3 1 18 3 2 19 3 2 1 19 3 2 2 19 3 2 3 20 3 2 4 23 4 25 4 1 25 4 1 1 UV 26 4 1 2 26 4 1 3
35. OO Y B 1 O ON 1 OFF O do store SYNC WASH 3 WASH 40 5 Evalutaion evaluation Ebaluation Evalutation Step Forward Step backward RE TENTION LOOP NO RUN PATH OO cOO OO RUN RUN
36. PLC 4 Method File Method File Calibration Cal Evaluation Evaluation Manual Manual _ Calibration 10 Min O ML 1 DL2 PUMP CAL AB REC CAL Cal Calibration IStep Foward Step backward X ML DL P 5OO ML Volume AB O 1 ml
37. thod File Step Foward Step backward Method File 0 1 23456789 B1 MEMORY LIFT 36O PROGRAM METHOD CONC B DELETE METHOD ML DU MIN COPY FROM TO CM MIN ML DL BANK COPY FROM PORT SET CALL MET LOOP TMS END OF LOOP FEED TUBE HOLD ALARM WASH AB SYNC AB 31 0O 1 2 3 4 5 6 7 8 9 B1 B No 1 5 do store FPLC 5 B1 5 10 O 9 50 2 7 O 1 3 4 56 89 B1 MEMORY LIFT 360 MEMORY Capacity LCC 501 Plus 958 PROGRAM__METHOD
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