取扱説明書 - 株式会社リプロセル
Contents
1. 20 2 8 20 2 Xeno free iPS Takahashi K et al Cell 131 861 72 2007 pH WWW reDrocell com ReproXF EDTA Accutase VTN N Laminin 5 Matrige SynthemaxII2. A2 1x10 cells 60 mm Q3 A3 Q4 60 mm 3 100 mm 1 A4 100 mm 30 60 mm 1 Q5 EDTA 5 A5 EDTA 5
3. 1 3 1 4 B2 ReproXF 60 mm 4 5 x 10 cells cm B2 1 A ReproXF 3 mL B2 2 PBS 4 mL B2 3 PBS Accutase 1 mL 37 10 B2 4 Accutase P 1000 15 mL B2 5 RerproXF 2 mL 15 mL B2 6
4. EDTA O6 EDTA A6 EDTA EDTA 2 85 https www reprocell com 1 WWW reDrocell com E mail info_jpQreprocell com For Research Use Only
5. iPS ALP 4 5 EDTA C2 Accutase 7 iPS 8596 Fig 3 4 5 C2 1 A ReproXF 3 mL C2 2 ReproXF PBS 4 mL C2 3 PBS Accutase 1 mL 37 10 C2 4 Accutase P 1000 15 mL C2 5 RerproXF 2 mL 15 mL
6. ReproXF 10 ng mL bFGF RCHEOT002 003 5 ReproXF Primate ES Cell Medium RCHEMD001 0 5 mM EDTA Vitronectin truncated recombinant human VTN N Invitrogen A14701SA Laminin 5 RCHEOT004 Corning 354277 ESGRO Complete Accutase Millipore SF006 Y 27632 Wako 257 00511 PBS Ca Mg free PBS 60 mm ReproCoat RCHEOT001 0 196 4 A A1 VTN N A2 Laminin 5 A3 A4 SynthemaxII SC B ReproXF B1 ReproXF B2 ReproXF C ReproXF C1 EDTA C2 Accutase D For Re
7. C1 9 ReproXF 12 mL 3 C1 10 A Laminin 5 VTN N 4 mL 10 uM Y 27632 Y 27632 C1 11 37 596CO gt C1 12 4 mL Y 27632 C1 13 4 5 85 Fig 3 4 5 85 Fig 3 ReproXF
8. C2 6 5 10 uL C2 7 300 xg 5 C2 8 C2 5 C2 9 15 mL ReproXF 1 x 10 cells mnL C2 10 51 mL C2 1 10 IM Y 27632 Y 27632 C2 11 37C CO C2 12 1 D Q1 3 859 A1 85 Q2 4 5
9. 10 uL B2 7 300xg 5 B2 8 B2 6 B2 9 15 mL ReproXF 1 x 10 cells miL B2 10 1 mL B2 1 10 IM Y 27632 Y 27632 B2 11 37 CO gt B2 12 1 B2 13 7 1 1 4 1 2 1 3 1 4 C ReproXF
10. ALP 2 ReproCoat ReproCoat 0 196 B1 1 Primate ES Cell Medium ES iPS 60 mm 3 B1 2 Primte ES Cell Medium PBS 4 mL B1 3 PBS Accutase 1 mL 37 10 B1 4 Accutase P 1000 15 mL B1 5 Primate ES Cell Medium 2 mL 15 mL B1 6 3
11. C1 EDTA 4 iPS 859 Fig 3 4 5 8596 C1 1 ReproXF PBS 4 mL C1 2 PBS PBS 4 mL C1 3 PBS 0 5 mM EDTA 2 mL 6 For Research Use Only po 3 ReproCELL 5 EDTA C1 4 EDTA ReproX
12. 1 15 mL B1 7 300 x g 5 B1 8 Primate ES Cell Medium 4 mL 2 B1 9 ReproCoat 10 uM Y 27632 B1 10 37 596CO gt 2 3 ES iPS B1 11 ES iPS 15 mL B1 12 Primate ES Cell Medium 2 mL
13. ES iPS 15 mL B1 13 300 x g 5 B1 14 ReproXF 4 mL 2 A B1 15 37 596CO 10 pM WWW reDrocell com Y 27632 Y 27632 B1 16 1 B1 17 3 1 1 4 1 2
14. 6 gt A3 5 A3 6 DMEM 11 6 mL 30 A3 7 30 60 mm 2 mL A3 8 3 4 WWW reDrocell com A4 Synthemax11 SC Reconstitution gt A4 1 10 mL Corning SynthemaxII SC Substrate 10 mL A4 2 1 mg mL SynthemaxII SC A4 3 SynthemaxII SC 4 6 gt A4 4 SynthemaxII SC
15. 40 0 025 mg mL SynthemaxII SC A4 5 SynthemaxII SC 60 mm 2 mL A4 6 2 A4 7 A4 8 4 3 For Research Use Only po 5 ReproCELL B ReproXF B1 ReproXF 60 mm 1 ES iPS 309 Fig 2 3 1 Fig 2
16. PBS A2 3 Laminin 5 10 Laminin 5 A2 4 4 37 2 A2 5 4 1 A3 lt gt A3 1 A3 2 15 mL A3 3 1 5 mL 400 pL A3 4 80 lt 60 mm
17. po 3 ReproCELL ReproXF Cat RCHEMDOO07 Day1 Day3 Day1 Fig 1 ReproXF ReproXF 3 30 Fig 2 1 x1 3 1 ReproXF 859 Fig 3 4 5x10 cells cm
18. F 2 mL C1 5 5 mL 15 mL 3 C1 6 ReproXF 2 mL C1 5 C1 7 ReproXF 2 mL C1 5 3 6 C1 4 C1 7 2 EDTA C1 8 100xg 2
19. search Use Only po 3 ReproCELL A A1 VTN N VTN N gt A1 1 VTN N A1 2 120 uL A1 3 80 lt 60 mm 4 gt A1 4 A1 5 15 mL PBS 8 mL A1 6 PBS 8 mL 120 uL VTN N 67 A1 7 60 mm 2 mL A1 8 1 4 1 A2 Laminin 5 60 mm 4 ug Laminin 5 A2 1 Laminin 5 4 A2 2 60 mm 2 mL PBS Laminin 5 vial
Download Pdf Manuals
Related Search