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ProX-tag

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1. Second PCR 4 5 T7 Promoter RBS ProX tag His tad ProX tag oROX FL92 1amber 4 pROX vector DROX FL92 1amDer Cat No TS011 DROX FL92 1cggg Cat No TS012 DROX FL92 1ttt Cat NO TS013 RYTSLinear Template Set for E coli ProX tag TT Promoter Xba CTAATACGAC TCACTATAGG GAGACCACAA CGGTTTCCCT CTAGAAATAA Ndel RB ProXtag TTTTGTTTAA CTTTAAGAA GGAGATATCAT ATGTCTAAAC AAATCGAAGT MetSerLysG InlleGluVa AAACTAGTCT AATGAGACCATG GENE GGGGGGGGTTC TCATCAT AsnXaaSer AsnGluThrMet GlyGlyGlySe rHisHis ProXtag C999 AACCGGGTCT AAT AsnXaa Ser Asn ProXtag ttt AACTTTITC AAT AsnPheSer Asn CATCATCATCATT AATAAAAGGG CGAATTCCAG CACACTGGCG GCCGTTA HisHIiSHiSHI 5 5 el 527 H CAAGGGCGAATTC CAGCACACTG GCGGCCGTTA CTAGTGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAACTAGC 5 Second PCR ProXtag amber RYTSLinear Template Set for E coli ProX tag 3 2 DNA Control Template CAT no 6 First PCR Second PCR First PCR Primer
2. 100 CloverDirect tRNA Me0 tRNAamber AU Acec _ UAG CGU AAU amp tRNAamber UAG mRNA 9 Release factor iO AGC UAG CGU AAU gt UAG Release factor 4 CCCG tRNA tRNAcccg Se Ge weaa Ge cs AGC CGGG CGU AAU tRNAcccg CGGG mRNA CGGG cli 3
3. 2 20 3 DNase RNase 4 PCR PCR PCR 5 p 6 RYTSLinear Template Set for E coli ProX tag 5 CloverDirect for Fluorescence Labeling CloverDirect CR110 X AF tRNA 5 CR110 X Abs Em 498 521nm CloverDirect HiLyte Fluor 488 AF tRNA HiLyte Fluor 488 Abs Em 497 525nm CloverDirect TAMRA X AF tRNA 5 6 TAMRA X Abs Em 546 575nm CloverDirect ATTO 633 AF tRNA ATTO633 Abs Em 629 657nm CloverDirect ATTO 655 X AF tRNA ATTO655 X Abs Em 633 684nm for Fluorescence Labeling CloverDirect Biotin AF tRNA Biotin CloverDirect Biotin X AF tRNA Biotin X CloverDirect Biotin XX AF tRNA Biotin XX for Post translational Modification CloverDirect i Tyr PO_H gt tRNA O
4. 4 E coli TAG TAG 4 DNA cf gi tRNA Z9 RYTSLinear Template Set for E coli ProX tag 7 URL httD www proteinexpress co jp 260 0856
5. 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mall tech proteinexDresS CO D
6. RYTSLinear Template Set for E coli ProX tag Step4 Second PCR First PCR Primer 2 Second PCR PCR Expand High Fidelity PCR System PCR uL PCR buffer 10xconc without MgCI_ 5 x1 10mM dNTP 1 25 250 uM 25mM MgCI 4 2 mM Forward Primer no 1 2 Reverse Primer no 2 2 Color Primer Set no 3 no 4 or no 5 1 First PCR Droduct 100 ng uL 2 200 ng Expand High Fidgity Enzyme mlx 0 85 3 U RNase 31 9 Total 0 PCR 2 Primer ProX tag armDber Forward Primer no 1 Reverse Primer no 2 Red Primer Set no 3 ProX tag Cd99 Forward Primer no 1 Reverse Primer no 2 Blue Primer Set no 4 ProX tag ttt Forward Primer no 1 Reverse Primer no 2 Yellow Primer Set no 5 Primer Set
7. Gene specific SenSe DrIImer 5 TCTAATGAGACCATGGAGAAAAAAATCACTGG Gene specific antisense Drimer 5 TGATGATGAGAACCCCCCCCCGCCCCGCCCTGCCACTC 3 First PCR Second PCR 1 5 6 0 5 1 2 uL First PCR Second PCR 0 5 1 2 0 5 1 2 ul 1 000 bp gt 800 bp gt 500 bp gt 6 First PCR Second PCR RYTSLinear Template Set for E coli ProX tag 3 3 C His tag CAT PCR pROX FL92ttt vector 5013 RYTS Kit F002 CAT 15 SDS PAGE His tag 7 PCR product Plasmid kDal 40 30 4 20 15 10 7 CAT 0 25 uL RYTSLinear Template Set for E coli ProX tag 4 First PCR Second PCR PCR 1
8. 1S002 Ir ProteinExpress RYTS Linear Template Set for E coli ProX tag 48 reactons Version 1 1 PCR Expand High Fidgity PCR System URL httD www DroteinexDreSS CO D 260 0856 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mall tech proteinexDresS CO D RYTSLinear Template Set for E coli ProX tag gt 2 2 ae 4 3 aaa 5 3 1
9. DNA ii 6 3 2 DNA ki 1 3 3 1 12 4 i 1 3 14 6 i 16 7 kk 17 RYTSLinear Template Set for E coli ProX tag 1 RYTS Linear Template Set 2 PCR T7 RBS 1 pROX vector N ProX tag 2 CloverDirect N
10. CCG GCC tRNA 6 dud CGG GUCUAAU me UAA Stop CGG tRNA 3 RYTSLinear Template Set for E coli ProX tag No 1 T7 Promoter Prime T7 Terminator Primer 2 3 Red Primer Set 48nL 1 ProX tag amber DNA 4 Blue Primer Set 48uL 1 ProX tag cgg9 DNA 6 Control Template 20uL 1 pROX FL92 1ttt CAT CAT 100ng uL Vector 20 PCR Expand High Fidelity PCR System Roche Applied Science 1732641 DNA Wizard R SV Gel and PCR Clean Up System Promega 9282 DNA RYTSLinear Template Set for E coli ProX tag 3 3 1 DNA
11. ProX tag TTT First PCR Gene SDecific sense Drimer Forward Primer Gene SDecific antisense Drimer no 1 Color Primer Set D gt no 3 no 5 Reverse Primer no 2 Second PCR RBS His tag T7 Promoter ProX tag 1 2 PCR PrOX tag ProX tag amber TAG ProX tag Poree Ge oo mr ProX t30 2 RYTSLinear Template Set for E coli ProX tag CloverDirect 1 UAG CGGG 4 CloverDirect tRNA UAG CGGG UAG Arg tRNA CGGG
12. p 10 RYTSLinear Template Set for E coli ProX tag Step5 Second PCR PCR DNA PCR PCR PCR PCR Forward Primer no 1 Reverse Primer no 2 PCR PCR PCR 4 PCR DNA RYTS Kit CFO001 CF002 Step6 DNA PCR ProX vector
13. RNase DNA 9ene specific SenSe primer Oene specific antisenSse Drimer PCR Expand High Fidelity PCR System DNA Wizard R SV Gel and PCR Clean Up System DNA DNase DNA RYTSLinear Template Set for E coli ProX tag Step1 Gene sDecific sense Drimer Gene specific antisense primer 1 1 ProX tag amber cggg ttt Gene specific sense primer 5 TCTAATGAGACC ATG 15 20nt sense Drimer Gene specific antisense Drimer 5 TGATGATGAGAACCCCCCCC 15 20nt primer RYTSLinear Template Set for E coli ProX
14. phospho Tyr CloverDirect Lys Me tRNA methyl Lys CloverDirect Lys Me tRNA dimethyl Lys CloverDirect Lys Ac tRNA acetyl Lys for Unnatural Mutagenesis PEG CloverDirect PEG4 AF tRNA Methyl PEG4 CloverDirect PEG8 AF tRNA Methyl PEG8 CloverDirect PEG12 AF tRNA Methyl PEG121 CloverDirect BPA tRNA p benzoyl phenylalaninel CloverDirect AcPhe tRNA p acetyl phenylalanine CloverDirect A azoAla tRNA p phenylazophenyl alaninel tRNA http www proteInexDreSS CO D products reagent Cloverdirect 1 htm RYTSLinear Template Set for E coli ProX tag Remarkable Yield Translation System Trial Kit RYTS Trial Kit CF001 0 3ml Remarkable Yield Translation System Kit RYTS Kit CF002 0 3ml X5 1 5ml 2 PCR RYTS Linear Template Set fo
15. tag Step2 First PCR 2 DNA First PCR PCR Expand High Fidelity PCR System PCR LL PCR buffer 10xconc without MgCI 5 x1 10mM dNTP 1 25 250 uM 25mM MgCI 4 2 mM Gene specific sense primer 10 uM 1 200 nM Gene specific antisense primer 10 1 200 nM LIM DNA 100 ng uL 2 200 ng ExDand High Fidgity Enzyme mlx 0 65 3 U RNase 34 9 Total 0 PCR Step3 First PCR PCR DNA PCR PCR PCR PCR
16. r c His tag TS001 48 RYTS Linear Template Set for c ProX tag TS002 48 pROX FL92 1amber TS011 pROX FL92 1cggg TS012 pROX FL92 1ttt TS013 tRNA http www proteInexDreSS CO D products reagent Cloverdirect 1 htm RYTSLinear Template Set for E coli ProX tag 6 tRNA tRNA NH2 A NH H0 P 0 2 0 9 pdCpA oko 4 Ei OH tRNA H2N R i pdCpA tRNA AUC 3 CA tRNA RNA

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