NanoDrop 8000 V2.3 User`s Manual 取扱説明書日本語版
Contents
1. 9 10 mm Absorbance cn ow CO lO ra RS rT 0 1 Section 8 Bradford2. fa 0 5 1 10 SUS303 9 1 Section 9
3. DNA DNA 1 2 DMSO 8
4. c NND 8000 13 Section Tools amp Configuratiion Data Default Administrator Default Default System Idle Timeout 4
5. 9 2 Section 70 10 1 uL 8 1 mm 0 2 mm 2048 CCD 220 750 nm 1 nm 3 nm FWHM at Hg 546 nm 0 003 absorbance 1mm 3 at 0 74 absorbance at 350 nm 0 02 75 Abs 10 mm 2 ng microliter dsDNA 3700 ng microliter dsDNA 20 24 x 32 cm 3 5 kg 303 12 Vdc 30 W 6 W Windows 2000 XP Vista 32 bit Windo
6. 1 mm Bradford pH 595 nm 0 150 A Bradford 3 5 26 AicroArray Eile Edit Configuration Help Section 5 Standard Methods Measure Blank Re blank Plate ID Sample Type ssDNA 33 Y Dye 1 Cy3 Dye 2 Cy5 1 lt 12 Hl OOOOOOOOOO Show Report User Default Date Time 9 9 2008 3 15 PM Measurement complete uda all Active n Off nm1 25l Units ndgul ctive 1 Sample 1 De 1 abs 0 351 Dye 1 pmaluL 23 40 ng ul SampleID Cu3 C5 nm1abs 0115 Due2abs 0638 Dwe2pmouL
7. 3 2 Section 3 Single Sample 8 Sample NanoDrop 8000 Single Sample 8 Sample 8 Sample NanoDrop 8000 V2 2 0 File Help User Detault Standard Methods User Methods Tools amp Configuration Single Sample CO d Sample Proteins amp Nucleic Acid Labels Protein Protein amp 28h BC Protein MicrnArray Bradfard Fratein Lnwry Cl Fratein Cultures Pierce bbll nm Single Sample 8 Sample
8. 7jp Tip 8 1 Calibration Check 1 PR 1 Calibration Check Calibration Check Tools amp Configuration gt Utilities amp Diagnostis gt Calibration Check 8 PCR 55 UL dH2O CF 1 8 1 5 UL dH2O
9. LapTop PC NanoDrop 8000 2 3 Section 3 3 1 8 Sample Single Sample
10. NanoDrop 8000 5 W Section 7 WEEE Waste Electrical amp Electronic Equipment WEEE 2002 96 EC Thermo Fisher Scientific EU WEEE
11. Bradford 2 uL 8
12. 2008 Thermo Fisher Scientific Inc All rights reserved Microsoft Windows Windows NT Excel Microsoft Corporation Adobe Acrobat Adobe Systems Incorporated Thermo Fisher Scientific NanoDrop Thermo Fisher Scientific 2010 10 KEF 1 1 1 1 1 1 1 1 1 1 WEEE A 1 2 1 2 2 1 2 1
13. 5 8 Section 5 Standard Methods NanoDrop 8000 100 pmoluL Cy3 750 ng uL DNA 48 replicates pmol uL pmol uL SD pmoluL CV Cy3 Cy3 5 Fs Alexa Fluor 555 0 25 100 Alexa Fluor 660 gt 4 0p 2 5 Cy5 Cy5 5 0 17 0 17 2 4 pmol uL 0 17 Alexa Fluor 647 gt 2 4 pmoluL 2 5 Alexa Fluor 488 0 50 8 0 pmoluL 0 50 Alexa Fluor 594 DRY gt 8 0 pmoluL 2 5 0 42 6 0 pmoluL 0 42 Configuration Measurement Limit 260 nm
14. s ug ml Show Report 200 Pierce 660 nm Pierce 660 nm Standard Reference Standard 7 Standard
15. Fax C NND 8000 Data gt gt BCA Protein Bradford Cell Culture Protein Lowry Proteins and Labels MicroArray Nucleic Acid Protein AS 280 UV Vis 8 10 Section 9 9 NanoDrop 8000 1 5 uL dH2O 2 2 3
16. 2 uL 8 30 40 NanoDrop PR 1 PR 1
17. 30 40 NanoDrop PR 1 PR 1 www nanodrop com 5 14 Section 5 Standard Methods NanoDrop 8000 20 mg mL BSA NanoDrop 8000 li RE 5 replicates SD mgl mL CV sample range 0 15 5 mg ml 0 15 mg ml sample range 0 25 4 pmol ul 0 25 Configuration Measurement Limit 280 nm
18. 340 nm 5 4 Section 5 Standard Methods Protein A280 A280 280 nm 220350 nm UV 280 nm A280 mg mL 0 2 mm A280 10 mm 1 cm
19. NanoDrop PR 1 1 PR 1 8 6 Section 8 2 PR 1 3 30 4 4 PR 1 PR 1 1 UL dH20 PR 1 1 2
20. 2 2 Standard linear interpolation polynomial fitting Hradford Standard Curves File Skiandards Help Print Page User Detsult 3 007 2 43 PM Reurm to Semple Acduis on Bradford uml IAve Abs Abs 1 Abs 2 Abs 3 Abs 4 Abs 5 100 8000 Adve A Reerencs 000 6041 0040 0040 0047 6047 004 i Acive B Standard1 10000 0048 0046 7 90051 0050 ud mL sexk e C Siandard2 20000 0064 0051 005 067 0063 0067 85 0096 0094 tiv Stan 2 20 0 119 D ve S 0 160 0 151 El G Serqd6 00000 6 5 018 100 1000 ug mL Meosurements Toble Double Click on any row to change he Concertration or delete replicate
21. e No Active Samples for Measurement Al Active 8 5 Section 8 e Error 8005 txt e Error 9000 passwords log passwords log C NND 8000 Data Log Files e Error 9003 1024x768
22. Measurement Type 6 1 Section 6 User Methods Standard Required Oligo Required 5 none Quantification Mode Single Sample Ale
23. 3 1 Section 3 Bradford 1 UL NanoDrop PR 1 8 e 1 UL s 2 UL es Bradford BCA Lowry Pierce Protein 660 nm Ass
24. DNA NanoDrop 8000 2 dye 0 2 pmoluL dye dye MicroArray dye Dye Chromophore Editor dye dye Dye Dye 260 nm 280 nm Dye 1 Cy3 Dye 2 Cy5 Dye Tools amp Configuratiion User Preferences
25. Bradford 2 uL 8
26. 2 uL dH2O OK Please wait Initioalizing Spectrometer Single Sample A File Edit Configuration Help Measure Re blank Show Repor User Defaul Date Time 9972008 2 46 PM Plate ID kak new BLANK kleasure ment Sample Type PO All Active DnyOf nm1 260 Units nul Blank F2 Blank 8 Blank Blank 8 Sample Blank
27. 1 USB NanoDrop 8000 PC PC 2 NanoDrop 8000 User Preference 3 Administrator PC 4 C NANODROP DATA USB _E USB 30 lt
28. SUS303 5 35 Section 6 User Methods 6 User Methods User Methods Single Sample 8 Sample
29. owry Standard Curves File Standards Help Pnn Page Magsuramenls Detfoult 3710 2008 2 13 PM Retum to Sample cq on 200C0153 0 3778 Table Double Ciick nn army row to change he concentration or deiete replicotes Standard Measure Blenk Actve Active Chive A Reterence B Standard 1 0 004 0027 _ 0 005 0 025 0002 0028 0005 0026 0 004 0028 C Standard 2 0 047 6045 1 0 045 0046 049 Acive D SWandard 3 0 5000 0 060 6074 0079 0 083 0 083 Actve Actve Cive Ciive Staridard Curve Absorbance Spectra 0 3 Slandard Curve Type Inmrpolaon FR snugred 0 9934 E Standard 4 F Standard 5 G Stondord b Stondard 7 Lowry Lowry 1 000 0 132 0130 0131 0133 Lowry 0 2 4 0 mg ml 35 40 45 50 55 60 65 70 75 maml 5 25 Section 5 Standard Methods Protein Bradford Bradford
30. mg mL Show Report 200 Baseline Type 2 750 nm 400 750 Slope Baseline Type 750 nm 400 750 nm A280 340 nm 5 16 Section 5 Standard Methods Protein BCA BCA Bicinchoninic Acid UV 280 nm
31. 2 uL 8 15 1 BSA 50 2000 ug mL 9 5 SD mg mL CV 6 15 1 50 ug ml 2000 ug ml 5 Standard Pierce 660 nm 60 uL 4 uL
32. NanoDrop 8000 3700 ng uL 1 mm 0 2 mm NanoDrop 8000 96 replicates ng uL ng uL SD ng uL CV 3700 dsDNA 2 5 3000 RNA 2400 ssDNA 2 5 100 ng uL 2 5 ng uL gt 100 ng uL 2 5 Configuratton Measurement Limit 260 nm Measurement Limit Sample Position llluminator
33. Standard Active Inactive Standard Standard Edit Standard Absorbance Replicate Edit one standard Standard Standard 5 mml 2 000 Awerage absorbance 0 151 Abs Replicates Delete Selected Reset Standard Standards s Standard 5 1 Standard Reference 2 Standard Standard
34. s mg ml Unit Show Report 200 Bradford BSA
35. nr8 1 Load Report Format 2 Excel C ND 8000 Data Reports Report s Method Date and Time s Report Name
36. Nucleic Acid 2007 03 21 nd8 2007 3 21 Nucleic Acid Data Viewer nd8 Data Viewer Protein A280 Protein amp Labels 1 0 cm 10 0 mm MicroArray UV Vis Protein BCA Bradford Lowry Cell Culture 1 0 mm 0 1 cm
37. USB USB USB USB 30 3 gt gt gt gt NanoDrop Devices Windows Computer Management Loacal Eiatteries Sywstem Tnnls a Biometric Ewent Viewer Bluetanth Shared Folders Computer Lnral Users and Groups Disk diwes FerFnrmanre Logs and alert Cisplay adanters Dewice Manager DPWEICD ROM ivwes 5korage Eh IDE TTAPT cnntrallers EF Remowable Storage IEEE 1394 Bus host controllers Disk Defraqmenter Keyboards Disk Management ice and other pointing Heyires Serwices and Applicatinns hi Modems E nnitnrs
38. CF 1 CF 1 KCrsO7 Thermo Fisher Scientific NanoDrop 8000 6 CF 1 260 280 Ratio NanoDrop 8000 260 280 260 280 3 pH 260 280 260 280 0 2 0 3 0 2 0 3 NanoDrop 8000 NanoDrop 8000
39. 2 uL 0 5 10 SUS303
40. 8 4 Section 8 Error Code 8 bi Error Code 8 Errnr reading or writing to file This might be caused by 1 The current Windows account does not hawe Read and Write priveleges to the folder CNanoDrop Data and all of ts suhfnlders Contactyour PC administratorto give all users Read and Write access to these folders 2 The log files have been remowed frnm the folder C lanoDrop Datalng es Replace the log files if they hawe been mowed lfthe Ing files can not be located reinstall this snftware 3 The log files described abowe are setto read only Check the propemes on each In file and ensure that the Read only hnx is unchecked c ND 8000 Data Windows PC Error Code 8013 Error Code 8013 ND 8000 Peripheral Control Device nat found 3 USB
41. UV 280 nm BCA Bradford BCA Cu BCA Bradford 595 nm 595 nm 750 nm Bradford
42. General Settings e Sample ID e 96 e Data Viewer User Default icroarray Proteins amp Labels Protein A280 eneral Settings Reports Data Exporting Nucleic Acids UV Vis Sample ID required 1OFF Automatic Column Advance 10FF Prompt Userto Close Data iewer OFF Data Exporting c NND 8000 Data Data Exporting Automatic Data Export Data Export Folder nd8
43. S MD T PC USB Connection Error PC USB PC PC Connection Error PC USB 8 3 Section 8 Signal Error Signal Error This is manstlikgly caused by Sample surface is dirty make sure surtaces are clean Sample arm make sure sample arm IS In down position No power to Instrument check 12w power supply For more details refertn he Troubleshooting section of the Users Manual avallahle from Main Menuj
44. Stat gt Programs NanoDrop gt ND 8000 version Standard Methods User Methods Tools amp Configuratiion 3 NanoDrop 8000 2 2 0 File Help User Default Standard Methods User Methods Tools amp Canfiguratian Single Sample CO 8Sample Prnteins amp Nucleic Acid Labels Protein Protein A258 BCA Protein MicroArray Bradford Protein US Lnwry Cl Protein Cultures Pierce BB5ll nm e Nucleic Acid e Protein A280 s MicroArray dye e UV Vis UV Vis e Cell Cultures e Proteins amp Labels dye s Protein BCA BCA e Protein Bradford Bradford s Protein Lowry Lowry
45. ND 8000 Data Viewer yi File Configuration Data Reports Help Testtype Nucleic Acid I A4372007 3 05 PM Plots Report Report Full Mode lgnore Pos 3123 2007 11 42 AM 530 57 12 611 z029 1 79 2 24 50 00 230 3 23 2007 11 43 AM 12 159 50 00 GOOng ul 3 23 2007 11 43 AM 11 903 3 50 00 GOOng ul 3 23 2007 11 42 AM 12 038 8 8 50 00 GOOng ul 3 23 2007 11 42 AM 12 076 i 3 50 00 600ngAul 3i23 2007 11 41 AM 12 212 8 50 00 BUngul 3 23 2007 11 42 AM 11 841 50 00 Start Report Recording Start Report Recording Report Table Recording Start Report Show Report File Edit Configuration tandards Help Show Report Data Viewer Report Exit
46. Description NanoDrop 8000 V2 0 File Help User Default v Exit Standard Methods User Methods Tools amp Configuration User Methods Descrnption Cy5 This is a protected method Cy dye used to determine the concentration based upon absorbance not fluorescence measurements Method Editor vv Double click to Run Method Method Editor Method Editor Sor hy Method Namg v hiethod List Method Quant Analysis Min Max Baseline Units Std Ext Factor Moal Mode nm nm nm Curve Coef Weight 2 Caffelne Standards 2 220 350 350 mg Linear LDH cgall cnunt Standards 9 230 750 75h cellssul Linear hy Note pratected methnds are indicated with a diamond and can nnly be modified hy their creator
47. Intensity Check Utilities and Diagnostics OK Intensity Check 8 2 65 USB Serial Number USB2G25234C0H15 B D E ulF 6 H 00 1 1 s 220n Zn 2400 250 2500 2700 2800 2900 3000 3i00 3200 339 34g 3500 Wavelenacih nm Visihle spectra 22h0 3000 FO annn ssbp smo ssno 6000 gs0 7000 7500 Wevelength nm Corfiguraton 014 48 8 12 20 Vdc
48. 8 1 Section 8 Found New Hardware Wizard Found New Hardware Wizard welcome to the Found New Hardware Wizard Windaws 0 search for current and upda oftware by This wizard helps you install software for CO on ME neharhe raillto n CD n indow Updse We lte with on NanoDrop USB Dewi rivacy polic ur hardw Can Windows connect to windows Update to search for Ws 56 di ks software O Yes this time only S se mane TS RS ation Adyal ced Click Next to continue Click Next to continue Intro Page Windows XP SP2 Other Windows Operating Systems 6 Add legasy hardware Windows 7 Add Hardware Update Driyer Software Ci Disable est3 4 i Uninstall pmputer Ek drives Scan for hardware changes Eplay adapters Add iegacy hardware D CD ROM drrves 6 ppy disk drives Propert ce bppy drive controllers Help man Interface Devices woE ATA ATAPT contrallers Disk Management Keyboards i Seryices and Apnlicatians Mice and other nointing devices Monitors Netwotrk adapters 4 5 Other devices yl PCT Serial Port PCT Simple Communications Controller Ther
49. Manual Sample ID Entry NanoDrop 8000 Sample ID Load Sample ID File Plate Files Sample ID Excel txt C WND 8000 Data Plate Files replicate txt 1 Sample ID
50. New Standards Measurements Measurements Tahlg Double Click nn any row to change the concentration nr delete replicates Bs CEBIT Da ou want tn Inad the concentrations and curve type for qenerating a standard Curve from a prewiously sawed standard Curye file Iwe ve E senaad4 ol ve F sendads ml Yes Standard No Standard 1 7 Reference 0 00 Standards File Edit Configuration Eicsiileslie Help Lnad frnm file Measure Blank Wiew or Measure Standards Show Report
51. Reference BCA Standard 7 Standard 5 5 18 Section 5 Standard Methods Choose Standards Source Standards are regquired Choose the Standard Curve source Load Standard New Standards CuryB Measurements New Standards Measurements Measurements Table Double Click on any row to nr delete SE Cates Do ou want tn Inad the concentrations and curve type for qenerating a standard Curve from a lv m we Esmndsd4 4590 mm macive Gsandade macwe Hsandad prewiously sawed standard Curye file Yes Standard
52. USB Instrument Not Found USB USB 30 Report Format Error c WND 8000 Dataicustom report format Error Code 8 PC e Source ErrOr
53. No Standard 1 7 Reference 0 00 Enter Standards Manually Standards Standard Active Inactive Standard Standard Edit Standard Absorbance Replicate Standard Edit one standard Standard Standard 4 ImdIml 5 veragde ah
54. No Standard 1 7 Reference 0 00 Standards Standards Help _ Lnad frnm fllg iew or leasure Standards Standard Active Inactive Standard Standard Edit Standard Absorbance Replicate Standard Edit one standard Standard Standard 4 morml 1 000 Awerage absorbance 0 249 bs Replicates Delete Se col q 0 250 electe 0 254 0 257 Reset
55. 3 5 Section 3 Single Sample Nucleic Acid Single Sample File Edit Cnnfiguratinn standards Help Re blank Show Repor User Defaul Date Time 8 19 2009 402 PM wa_ Measurement complete SampleID DNAsample5 Measurement Results Units ngAul Ns 280 0 779 nm 1 abs 1 448 eg 1 85 Sample Type EO NE lt A 250 1 448 260 230 200 ngul nm 1 260 Overlay control Clear graph now Legend 11 28 DNA sample 5 10 00 DNA sample 5 900 F DNA sample 4 a DNA sample 3 2lzlzlel2l2 i NN EE DNA sample 1 400 3 00 2 00 7 ac SS 1 da 1 1 1 1 1 1 1 1 220 230 240 250 260 270 280 290 300 310 320 330 340 350 Wrawelength nm 2 1 0C0868 0 20 56 Load Next Sample ID F7 Sample ID txt Sample ID Legend Sample ID Accumulate until clear
56. BCA Standard BSA mg mL Standard BSA NanoDopr 8000 BCA BCA
57. Directory tree Select Deselect Selected Samples Selected Samples 1000 Search Sample ID Sample Information Import and Return Plots Report
58. NanoDrop 8000 NanoDrop NanoDrop 8000 50 NanoDrop 8000 1 UL UV VIS NanoDrop 8000 3700 ng uL dsDNA DNA 100 mg mL BSA Bradford BCA
59. RoHS www thermo com WEEERoHS NanoDrop 8000 6 628 382 6 809 826 Section 2 2 IBM PC s Microsoft Windows 2000 XP Vista 32 bit Windows 7 32 64 bit NanoDrop Windows NT 95 98 ME 800 MHz CD ROM 128 MB RAM 100 MB USB NanoDrop8000 USB Microsoft Excel Windows XP
60. mg mL BSA NanoDrop 8000 Configuration Measurement Limit 660 nm mg mL Measurement Limit Sample Position Iluminator Plate Summary Plate Summary Sample Position llluminator
61. Excel Excel Export Data by Row 8 Sample A1 H1 A2 H2 A1 A12 B1 B12 15 Section Tools amp Configuratiion User Preferences File Help User Default Micrnarray Prateins amp Labels Pratein A280 General Settings Reports Data Exporting Nucleic Acids UY vis To expor NanoDrop S000 data automatically atter each measurement check the Automatic Data Expor box select a Data Export Folder Optinally configure custom expor data format for one or more test modules by first selecting a module and then clicking on the Configure Data Export for Module button Automatic Data Export on Export Data By Row on Test odule Data ExportFolder 1 Nucleic Acid CMDocuments and Settingsiydawve lachy MicroArray Desktap UY Yis Protein 280 Cell Culturg Proteins amp Lahgls BCA Prot
62. Measurement Limit Sample Position llluminator Plate Summary 3 Plate Summary Sample Position lluminator 0 9 AicroArray _ File Edit Configuration js Help Section 5 Standard Methods Measure Blank Re blank Show Report User Default DaterTimg 9 9 2008 3 15 PM Plate ID Measurement complete uda Sample Type ssDNA 33 v All Active On Of nml 260 Units ngul i ctive 1 Sample 1 De 1 abs 0 351 Dye 1 pmaluL 23 40 ng ul SamplelD Cy37C5 mm1aps 0115 Dye2abs 0638 Due2pmouL 2553 33 19 Acive 1 BP sample 1 Dwe1abs 0351 Dye1pmaluL 2337 Ne SamplelD Cy37C5 A mm1 abs 0117 Dye2abs 0639 De2pmauL 25 58 34 86 Acive 1 CP_ Sample 1 De1abs 0352 De1pmauLl 2347 Dye
63. 10 1 Section 70 Dye Chromophore List Editor DyeChrnrnnphnre List Name Thd cm nm qal 2 nm 280 nm factor factor IT5Ers 550 nnnEx 000 00 Below 250Er5 550 n00Exd 000 loon AlexaFluor 88 10E 4 495 D OOE D 0 00 0 00 Delete 4 Alexa Fluor 546 104E 5 556 OOOEXO 000 oo00 4 Alexa Fluor 555 T5OEx5 555 nn0ExO 00O om Alexa Fluor 594 730E 4 590 000E 0 00o0 ooo Alexa Fluar 647 239Ex5 650 00OExO oo ooo 4 Alexa Fiuor 50 13ZEx5 663 nnnExd 00O ooo Gy35 T5OEx5 581 nn0ExO 00O om Cy55 50E 5 675 Test w Note predetined es are indicated with a diarmnnd and cannot be moditied c A e b c ng uL A AU e ng cm microlite b cm e Double stranded DNA 50 ng cm ul Single stranded DNA 33 ng cm ul RNA 40 ng cm ul NanoDrop 8000 1 0 mm 0 2 mm
64. s nm 1 abs1 nm 2 abs2 1 mm 300 nm 700 nm e Normalize 400 700 nm s Show Report 200 Section 5 Standard Methods Proteins amp Labels A280nm fluorescent dye metalloprotein dye Proteins and Labels dye 10 Tools amp Configuratiion Dye Chromophore Editor
65. Data Viewer s Reports Report e Print Window Print Window CTL P Plot Report Standard e Save Window JPG 4 Import Data Viewer Plots Report Data Import Samples Import Folder Directory Tree 7 2 Section Tools amp Configuratiion Import folder
66. Bradford 1 UL NanoDrop PR 1 CF 8 6 CF 8 1 3
67. 1 USB 2 NanoDrop CD PC CD nd 8000 install exe 3 5 USB Found New Hardware Wizard Windows XP SP2 No not this time OS 4 Found New Hardware Wizard 2 1 NanoDrop 8000 1 2
68. 1 5 2 UL 8 1 mm NanoDrop 8000 10 mm 10 600 nm 0 5 1 10
69. 3 4 1 5 2 UL 1 5 2 UL NanoDrop
70. Calibration Check Help CF 1 Target Absorbance Calibration Check warning 10 e CF 1 45 10 Measure CF 1
71. Single Sample 8 Sample Automatic Path Selection 1 25 1 0 mm 0 2 mm 6 5 Section Tools amp Configuratiion 7 Tools amp Configuratiion Data Viewer MS Excel
72. 1 txt 1 Sample ID Manual Entry Enter Sample IDs Sample ID replicate Enter sample IDs Sample IDs Enter the Sample IDs via the keybnard nr barcode scanner SampleID Add Sample ID Sample ID Sample ID Add sample ID Enter Sample ID Sample ID
73. Configuratton Measurement Limit Measurement Limit Sample Position lluminator Plate Summary Plate Summary Sample Position llluminator Cell Culture ll _Edi _Canfiquratin 5 Help ea Measure Re blank Show Report User Defaul Date Time 9 9 2008 3 27 PM Plate ID In Shec keasurement complete AActtye On Of nm1 340 Active 1 hm1abs 0 477 600 0 090 SamplelD Active 1 nm 1 abs 0 463 A 600 0 080 Sample ID Active 1 C1 Sample 1 nm 1 abs 0 450 A 600 0 075 Sample ID 2 Aclive 1 D1 5
74. s Report Full mode Report Full Mode Print Save Print and Save 1000 Ignore s Max Report Size 1000 Standards Standards File Configuration Data Reports Help Plots Report Standards Test type Bradford 10 27 2005 1 17 Standards Sample Curve Ref Ref Std 1 Std 1 Std 2 Std 2 Std 3 Std 3 Std 4 _ ID Type conc Abs conc Abs conc Abs Conc bs Conc Reference Interp 000 0 029 NaN NaN NaN NaN NaN NaN Na Standard 1 Interp 000
75. 5 28 Section 5 Standard Methods es Standard 5 1 Standard Reference 2 Standard Standard Bradford Siandard Curyes File Standards Help Default 4123 2007 10 01 AM Return to Sample Acquisition 1 0 CD103 0 55 28 8 Measurements Table Double Click on any row to change the concentration or delete replicates Standard ugiml Ave Abs Abs 1 Abs 2 Abs 3 Abs 5 A Reference 00 0047 0041 0054 nn49 0 045 Standard1 1000 0057 0052 0059 0057 0059 C Standard 2 200 0 Ii 0 060 Standard 3 500 0 0 096 0 090 E Standard 4 1000 0 0 124 0 122 F Standard5 2000 0 0 144 0 137 G Standard5 4000 0 0 179 0 168 0 185 0 176 0 129 H Standard 7 8000 0 0 205 0 195 0 206 0 204 0 211 Standard Curve Absorbance Spectra chA Ch B ChC A Ch D IA ChE ChF Ch G IA 650 675 wwavelength
76. 5 User Preference Auto Column Advance Automatic column advance will DCccur in 4 8 seconds dwance nluimn Now Data Viewer e Prompt Close Data Viewer Data Viewer e Measurement Limit Measurement Limit 4 4 Section Sample ID List Format Enter Measurement Limits i Enter the Minimum and Maximum Concentration Sample measurements that fall nutside this range will be highlighted in displays on Min Canncentratinn 1 000 Max Cancentra
77. Edit method parameters Measurement Type wyavelenqths Baseline Namg The cnncentratinn is Calculated by multiplyind the absorbance A atthe nalysis wawelength by the Factor and dividing by the path length Bath the nalysis nm and the Factnr are reguired Quantificatinn Mode c FactorxAb Factorwalue ngAu 1 00E 0 Analysis Wavelength nm 300 Units ng Mol weight gmalh 0 00E 0 Edit View Selected 6 4 Example custom File Edit Cnnfiguratinn Help Section 6 User Methods Ia ee Default DaterTime 8 12 2009 9 48 AM Ce Pl
78. Retry USB USB 30 PC USB Retry USB Retry USB gt UPS
79. 0 5 10 SUS303 10 2 SS 130 0021 3 9 2 Tel 03 5625 9711 Fax 03 3634 6333 532 0003 5 1 3 403 Tel 06 6394 1300 Fax 06 6394 8851 E mail webmaster scrum net co jp Internet www scrum net co jp NDT141205A
80. CF 1 1 5 L Tip CF 1 CF 1 1 5 L 15 20 CF 1 CF 1 13 Measure 14 CF 1 11 12 5 5 Calibration Check JPG TXT ND 8000 Data Calib check Calibration Check
81. e Low Detector Bias Driver X Configuration Failed You Must Manually Edit the Registry Windows 2000 XP PC Insufficient Memory 100 MB Warning The ratio between the long nath and shart nath ahsnrbance is out of tolerance This might be caused by 1 an air bubble was entrained in the sample Try measuring againi 2 The liquid column is not Forming can be confirmed wisually IF this i
82. NanoDrop 8000 3 USB Hardware Wizard 2 2 1 Section 2 Windows Device Manager Computer Management Local 0 System Tools Event Viewer Shared Folders Computer Local Users and Groups ge Disk driyes Performance Logs and Alerts Display adapters Device Manager DVD CD ROM drives gg Storage amp Human Interface Devices RemovaDle Storage IDE ATA ATAPI controllers Disk Defraqmenter a IEEE 1394 Bus host controllers eg Disk Management Keyboards Services and Applications Mice and other pointing devices Modems Monitors NanoDrop Devices LED Positon Indicator ND 8000 Peripheral Control Device ND 8000 Spectrophotometer Network adapters MS Sa
83. Serial Number USB2G32185CPE1 Padastal H Detector Bias 354 UV Spectra 4100 0 gt 3500 0 3000 0 2500 0 000 5 20000 1500 0 1 1000 0 500 0 00 2200 2300 2400 2500 2600 2700 2800 2900 3000 3100 3200 3300 3400 3500 We Visible Spectra 4100 0 35000 3000 0 Max Single Offsat 0 54 2500 0 5 20000 Xenon Peaks Monitored _15000 4W 4840nm 4845 1000 0 1 Aa 850 7 nm 2605 000 MM a bs 362 5nm 362 1 823 2 nm B23 2 00 2200 3000 3500 4000 4500 5000 5500 E000 6800 7000 7500 8O00 8600 Wavlendth nm Configuratiom 0 09 40 Account Management Account Management Account Management Administrator User Access Manager Actions allUsers FulName Default Defaultu ctiwe Not Locked NotExpired Mewer Administratnr ne Not Locked Nat Expired Never 3
84. Measurement Limit Sample Position llluminator Plate Summary Plate Summary Sample Position llluminator 5 15 Section 5 Standard Methods Proteins amp Labels HD File Edit Configuration Help Measure Re blank Show Report User Default Date Time 9 9 2008 3 27 PM Plate ID 10 plates DNA 1 Measuremen t complete Sample Type BSA All Active OnyOf nm1 280 Units mgiml Acti 1 A ctive Sample 3 Dwe1abs 4505 Dye1uML3006 ml SamplelD BSA cy3 nm 1 abs 5193 Dye2abs NaN Dye 2uM NaN 7 569 Baselin 1 AlegOOOOOOODOOODD is EL F1 sample l 2 Dwe1abs 4271 DyetuM 2847 nm B GO QO Sample ID BSA cy3 nmlabs 4954 Dye2abs NaN Due2uM NaN 7 281 Acive 1 G1 sample 2 Delabml 4252 Dye1uM 2835 pl O OOOOOOOOOO
85. mg mL BSA NanoDrop 8000 Coomassie G 250 Pierce Coomassie Plus Protein Assay 3 Coomassie Plus Protein Assay G 250 Bradford Bradford 5 27 Section 5 Standard Methods
86. 8 30 40 NanoDrop PR 1 PR 1 www nanodrop com 20 1 NanoDrop 8000 BCA 0 20 mdg mL 8 0 mg mL 0 01 0 20 mg ml Configuration
87. Nucleic Acid A280 Proteins amp Labels 10 mm 0 05 abs 6 Configuration Sample ID Blank Measure F1 Measure Blank Measure F1 20 8 Measure Blank F1 Measure Measure Sample Position llluminator 96
88. All Active On Measure F1 8 0 8 8 Section 8 1 mm 0 005 A Nucleic Acid A280 Protein amp Labels 10 mm 0 05 abs
89. Oligo Calculator Oligo Calculator Oligo Calc Oligo Property Calculator Oligo Seq AAATTTCCCGG Nucleic Acid hhNA Modification Weight no Phaspharylated Dauble stranded Mol Weight qirmol 3 545E 3 GC 50 00 Ext Coetf ImnlFcmij 1 280E 5 Conc Factar hd 28 48 fhasgs 12 Section 5 Standard Methods Oligo Calculator 750 nm 400 750 nm 5 11 Section 5 Standard Methods UV VIS UV VIS Absorbance NanoDrop 8000
90. 10 0 mm 50 Protein A280 Protein amp Labels 1 0 cm 10 0 mm MicroArray UV Vis Protein BCA Protein Bradford Protein Lowry Cell Culture 0 1 cm 1mm NanoDrop 8000 methanol ethanol n propanol isopropanol butanol acetone ether chloroform carbon tetrachloride DMSO DMF acetonitrile THF toluene hexane Dbenzene sodium hydroxide sodium hypochlorite bleach dilute HCI dilute HNO3 dilute acetic acid HF
91. Administrator Account Management Options Modify User Password Expiration enabled Default Expiration Days Options Maxirmurm PasswnrdAttempts 100 Minimurm Passwnrd Length 0 Password Expiration enabled a Default Expiration Days 360 Passwords log c NND 8000 Data log files Passwords log ID Administrator Passwords log Administrator 7 14 Section Tools amp Configuratiion passwords log Password log User Preferences
92. Measurement Limit Sample Position llluminator Plate Summary Plate Summary Sample Position llluminator 5 Protein A 280 _ File Edit Canfiquratin Help Section 5 Standard Methods xX Measure Blank Re blank Show Report User Defaul Date Time 9 10 2008 9 45 AM Plate ID BSA Measurement complete ua SampleType BSA y All Active On Of nm1 280 Units mgiml W Active A Sample 1 nm 1 abs 1190 260 280 074 ml SamplelD BSA A 280 1 195 1 733 ls Active 1 B1 Sample 1 nm 1 abs 1 205 260 280 075 ra md ml Sample ID BSA A280 1 214 1 821 Aclive 1 C1 Sample 1 m1 abs 1 181 260 280 073 Nt Sample ID BSA A280 1 185 1 779 Actve 1 AAA D1 sample 1 nm 1 abs 1 258 260 280 0 74
93. PR 1 s s NanoDrop 8000 0 5 10 L 8 dH2O CF 1 s Tip cc 7 8 Section Tools amp Configuratiion Fisher 0 1 10 L low retention tip cat 02 717 134 8 Dischage and Touch 1 CF 1 Calibration Check
94. Section Tools amp Configuratiion CF 1 s CF 1 e e 10 Tip Discharge and Touch e 8 lt Tip s 8 es 5 CF 1 s
95. 1 10 mg mL BSA 280 nm 6 7 IgG 1 10 mg mL IgG 280 nm 13 7 Lysozyme 280 nm 26 4 M cm M W e 999 X 1000 M W 9999 X 1000 1 10 mg mL L gm cm BS5 amp v Ia WW sz1000 1 00 Mal wweight kDai 1 000 Sample Type CherproienmElo Other pratein IE 1 Ext Coeff E 1 Li m cm
96. Reference Lowry Standard 7 Standard 5 Choose Standards Source Standards are regquired Choose the Standard Curwe source Load Standard New Standards CUPwe Measurements New Standards Measurements Do ynu want tn lnad the concentrations and curve type for RUNS SRR CHVE HINA we Erseredd am previously saved standard curve file ve Ee Sendard4 we F Sendard5 emm Yes Standard No Standard 1 7 Re
97. 50 1 UL dH20 The detectaris saturated for the following channels This is mnstlikely caused by initialization with ty measurement pedestals lfthe error persists Clean the pedestals exit the snftware and restart
98. 10 mm 1 mm 10 0 2 mm 50 260 280 260 nm 280 nm DNA RNA DNA 1 8 RNA 2 0 280 nm 260 280 5 3 Section 5 Standard Methods 260 230 260 nm 230 nm 260 230 260 280 1 8 2 2
99. Data Viewer Data Viewer Plots Reports Standard Curves 2 3 Show Report Report Show Report Data Viewer Start Report Recording 3 s File jpg s Configuration Auto Scale Include graph in printout Include standards in printout se Data
100. Legend s Sample information Sample Information Data Viewer s X Y X Y Cursor Abs X Y Reset Baseline X 0 7 4 Section Tools amp Configuratiion Report Report
101. e Protein Pierce 660 nm 660 nm Single Sample Section 5 Standard Methods NanoDrop 8000 Nucleic Acid 1 uL 1 5 2 UL 8
102. Lowry Pierce Protein 660 nm UV VIS 8 1 uL 1 mm 0 2 mm 8 CCD PC PC NanoDrop 8000 4 4 37 8 C 10 90 NanoDrop 8000 12V
103. Columns 8 At1 H2 8 A2 H2 3 4 96 plate plate 4 2 Define Sample ID File Format Enterthe Settings fnr Format Sample ID Files Use Rehlicates Calumn is checked you must enter the Sample ID File calumn that hnlds Ihe nf Replicates lfleft unchecked of Rehnlicates will belftorall samples in the Sample ID File Help 2 Data Series Type In Columns ALB1 Sample ID Column 2 of Header Rows 4 gt Use Replicates Column Sample Replicates Column 3 Sample ID File Raw Sample ID File Data 3 lsmmm2 lj hh me3 ll hh Smet ll hn me 3 hl Smee ll samer ll hh hh lsmmme 3 1 hh ame 3 ll hh Same ll Smell ll CAND 8000 DatalPlate flesExample td _Column 1 Column 2 Column 3 well position Example Sample ofreplcates Section Sample ID List Format gt iM i Show Sample to Well Position Assignments SampleiDs 1 14 15 js 8 10 11 5 5 3 11 12 Sample 1 Sampled Sampe17 Sanple25 ampe33 Sanple41 Sampe4
104. Passwords lIog Administrator passwords Ilog Password log Dye Chromophore Editor Dye Chromophore Editor dye MicroArray Proteins and Labels dye chromophore dye 260 nm 280 nm dye Absorbance contribution dye decimal correction dye dye DyeyChromophore List Editor Dye Chramaphnre List Namg 1M cm nm qal 260nm 5 280nm 2 Insert Cy3 50E 5 55H HHHE H HH 0 05 50E 5 650 000E 0 000 005 AlexaFluor488 10E 4 0 11 Alexa Flunr 545
105. A 2 3 PC 4
106. Single Sample Configuration Sample ID Palte Setup Mode Configuration Configuration 2 s Sample ID Required Sample ID 4 1 Section Sample ID List Format e Prompt Close Data Viewer Data Viewer Sample ID 8 Sample Sample Loading Mode Plate Setup Select Sample Loading Mode Lnad Samhle ID Manual Samhle ID File Entry Define Sample ID Cancal File Fnrmal Sample ID File NanoDrop 8000 Sample ID
107. 12 V NanoDrop 8000 12 V 2 2 Section 5 W 12 V NanoDrop
108. Auto Reporting Reports Auto Reporting User Preference Save amp Exit Auto Reporting User Preference log Iog User Preference Iog ID c ND 8000 Datauog files Administrator
109. OK Tip 8 1 5 UL Blank Target absorbance 350 nm 1 mm Pathlength CF 1 Target Absorbance Target Absorbance CF 1 lt 9 Section Tools amp Configuratiion 10 CF 1
110. 1 Blank Blank 3 3 Section 3 Blank Blank 1 2 3 Blank 4 AlIActive On Measure F1 8 0 5 1 mm 0 005 A
111. Sample Position llluminator 5 22 Section 5 Standard Methods Lowry File Edit Configuration Standards Help Re blank Show Report User Defaul Date Time 9 10 2008 2 19 PM Plate ID Lowry Measurement complete Standards Ade en nm1 6550 Units ViewiUpdate Standards sg Active 1 2 Sample 2 nm1abs 0195 405 0083 mgiml SamplelD Loww Le A 650 195 2 629 ctive 1 2 B 5ample 2 nm1 abs 0193 405 0 096 EL SamplelD Loww AB5D 0193 2 467 active 1 2 1 2 Sample 1 nm1abs 0 221 A405_0118 mgyml SamplelD Lowy Lo amp A 650 0 221 7 747 acive 1 D2 fsampet 1 jnmiabs 0214 4405 0121 om SamplelD Lowry Le Ar5H 0214 4 651 1 Aaclive 1 Ez et 1 nm1 abs 0 216 405 0 123 mgrml A SSDOOOOOOOOCI ee 1 2 Sampet 1 mlspmln215 A405 115 om BI OO OOOOOO sanpeD Lowy 650 0216 5 573 active 1 ample 1 nm1 abs 0217 405 0110 mgrml EC CCCCCICICITCICI Sanpepl Low 650 0 217 5 967 active 1 ample 1 nm 1 abs 0217 405 0142 nl OO00OOO00OOOI eee niml aa Err 2 0 0 CO158 0 37 484 s A650nm 650 nm Cu 1 mm
112. Sample Position llluminator Sample Status Color Code Sample Position llluminator Sample ID Re blank F3 Reblank Blank Re blank Blank Start Report Recordind Start ReportRecording Active Report Table Recording Start Report
113. 12 Sample ID 12 Sample ID Legend 8 Sample Nucleic Acid File Edit Canfiquratin standards Help Re blank Show Report User Default Date Time 9 9 2008 2 21 PM Platia ID 425 ng ul Measurement cnmhlete ud Sample Type ESGNS5 lt All Active On Of nm1 260 Units ngul Active 1 A3 Sample 1 nm1abs 8811 A260 8811 uu SamplelD 440ngAul A280 4749 260 280 1 85 260 230 219 Active 1 A 63 sample 1 mtabs 8855 A260 8856 al SanglelD 40reou Azn 255 nzenl iss amnl zis ia Active 1 N C3 Sanpe 1 mtabslB8764 4260 8764 ui SamplelD 440 ngsul A280 4725 260 280 1 85 260 230 217 _ 82 active 1 D3 Sample 1 nm1 abs 8781 A260 8781 ul SamplelD 440ngAul A280 4726 260 280 186 260 230 219 _ 91 Active 1 E3 Sample 1 nm1abs 8782 A260 8782 al SamplelD 440ngul Ar280 4702 260 280 187 260 230 219 _ 4391 1 L3 12 Al OOOOOOOO active 1 N F3 Sample 1 nmlabs 8769 A260 8769 al BI OOOOOGOQI 5ammeip Monga
114. 2 Measurement Limit Sample Position llluminator Plate Summary Plate Summary Sample Position llluminator 5 12 Section 5 Standard Methods A File Edit Configuration standards Hep Measure User Defaul Date Time 9 9 2008 2 28 PM Plate ID Dichnmate Utomatic Path Selection On Measurement complete nm1 350 nm2 700 Aclive 1 1 Sample 1 nm 1 abs 0745 nm 2abs 0002 Sample ID unknown 1 ES Active 1 BT Sample 1 nm1abs 0 745 hm 2 abs 0 006 Sample ID unknown 2 Active 1 CT Sample 1 nm 1 abs 0734 nm 2 abs 0 001 Sample ID unknawn 3 Acive 1 DT Sample 1 hm 1 abs 0741 nm 2 abs 0D 001 Sample ID unknown 4
115. 1 15 4 50 1 51 4 00 1 47 260 280 4 260 280 DNA RNA 1 8 2 0 RNA 6 9 Section 8 260 280 Leninder A L Biochemistry 2nd ed Worth Publishers New York 1975 Utilities and Diagnostics JPG Utilities amp Diagnostics OK Intensity Check
116. Protein A280 nm 1 nm 1 abs Dye 1 dye Dye 2 dye Dye 1 2 abs dye 10 mm Dye Dye 260 nm 280 nm Dye 1 Cy3 Dye 2 Cy5 Dye Tools amp Configuratiion User Preferences Concentration ng ul 280 nm Units
117. 30 40 NanoDrop PR 1 PR 1 www nanodrop com NanoDrop 8000 Lowry 0 20 mg ml 8 0 mg ml Configuration Measurement Limit 650 nm mg mL Measurement Limit Sample Position llluminator Plate Summary Plate Summary
118. File gt Save Window Seral Number USB2G25679C0019 Alt Print Screen Fn Alt Print Screen MS Word MS gt jpg doc
119. NanoDrop 8000 Lowry Lowry Reference Lowry Standard 7 Standard 5 5 23 Section 5 Standard Methods Choose Standards Source Standards are regquired Choose the Standard Curve source Lnad Standard New Standards CuryB Measurements
120. www nanodrop com NanoDrop 8000 Bradford 100 ug mL 8000 ug mL 100 1000 ug mL mini Bradford 15 ug mL 125 ug mL Configuration Measurement Limit 595 nm mg mL Measurement Limit Sample Position llluminator Plate Summary Plate Summary Sample Position llluminator
121. 55 UL 8 PCR 55 L 200 L 11 8 1 5 uL CF 1 Tip CF 1 1 5 L 8 1 CF 1 CF 1 8 CF 1 1 5 L 12 Discharge and Touch 1 5 uxL CF 1 Tip CF 1 1 CF 1 CF 1
122. es nm1andnm 1 abs s mg ml Unit Show Report 200 Lowry BSA mg mL BSA
123. dye dye NanoDrop 8000 2 dye 0 2 uM dye 280 nm Cy3 dye None None dye dye dye Dye Chromophore List Editor DyeChrnrnnphnre List Namg 1M cm nm qal ebll nm 280 nm factor fctor Insert gt 8 TS0Er5 550 90E0 omo lo 4 6 zs0Er5 650 00ExO oo ooo e Alexa Fluor 488 7 10E 4 495 OOOE D 00O 000 Alexa Fuor 546 194E 5 556 00OExO 000 000 eeded gt Alexa Fuor555 ILS0Es 555 900Ex0 om 900 gt Algxa Fiuor 594 30Ex4 590 000ExO 000 000 lt Alexa Fuor647 5 550 5OGExO ni 900 lt Alexa Fluar 660 32E5 563 00GExO ono oon lt os OE 581 5OGExD ono lo Os 575 Q00E Tast alm m oj o
124. 220 nm 750 nm 1 uL 1 5 2 UL 8 NanoDrop 8000 1 mm 2 mm 7 5 A Configuration Measurement Limit
125. 8 x 12 txt 8x12 Sample ID 1 8x12 Plate Map Define Sample ID File Format 8 x 12 Sample ID Load Sample ID Define Sample ID File Format 8 x 12 txt Define Sample ID File Format Plate Map txt Sample ID Plate Files Data Series Type rows or columns Rows 12 Al1 A12 12 B1 B12 C D
126. Plate Summary Plate Summary Sample Position llluminator 5 2 Nucleic Acid _Edit Configurat ap SS ITTTTTsmnmn Tj mm5R RpTR p Measure Blank Re hlank Show Report User Default Date Time dr2008 2 21 Pk Plate ID 425 ng ul Measuremen tcomplete Sample Type Ml Lv All Active On Of nm 1 260 Units ngul Active 1 4 A3 Sample 1 nm1abs 8811 260 8811 it samplelD 440ngAl A280 4749 260 280 1 85 260 230 219 42405 Aclive 1 B3 Sample 1 nm1 abs 885E 26 8 856 Be SamplelD 440 na ul A280 4765 260 280 185 260 230 219 4428 1 Active 1 C3 Sample 1 nm 1 abs 8764 A 260 8 764 NE SamplelD 440noul A280 4725 260 280 185 260 230 217 4382 Aclive 1 D3 sample 1 nm1abs 8781 A 260 8 781 yi SampleID 440ngl A280 4726 260 280 186 260 230 219 4391 active 1 E3 Sample 1 nm 1 abs 8782 A 260 8 782 SamplelD 440noul A280 4702 2607280 187 260 230 219 91 1 3 12 Acti F3 N Al OOOOOOO ctive Sample 1 nm 1 abs 8 7
127. s Concentration ng ul 260 nm Units ng uL Show Report 200 Oligo Calculator Oligo Calculator Oligo Calc Oligo Property Calculator Oligo Seq AAATTTCCCGG Nucleic Acidl DNA wv Modification Weight 00 Phaspharylated Double stranded Mol eight qimol 3 545E 3 GC 50 00 Ext Coeff lifrmol crm 1 280E 5 Conc Factor hdh 28 48 fhases 12 Oligo Calculator
128. CF 1 e e Blank Reset e Blank 7 11 Section Tools amp Configuratiion Calibration Check File Load Cal Check File Help 12 Section Tools amp Configuratiion Intensity Check Intensity Check
129. Measurement Limit 562 nm mg mL Measurement Limit Sample Position llluminator Plate Summary Plate Summary Sample Position llluminator 5 17 Section 5 Standard Methods BCA Protein File Edit Configuration Standards Help Re blank Show Report User Default Date Time 9 10 2008 11 32 AM Plate ID BCA Measurement complete le Re AActiyg OnAOT nm1 562 Units mg ml wiewyUpdate Standards active 1 Al 5Sanple 1 nm1 abs 0 046 zz50 0016 nil Sample ID BCA amp Aa 552 0 046 0 9893 Active 1 B1 Sample 1 nm 1 abs 0056 Aa750 0019 Sample ID BCA active Sample ID BLA Active Sample ID BCA amp Active
130. Report Report ND 8000 Data Viewer yi File Cnnfiguratinn Data Reports Help Plots Report Testtype Report Name ReportFull Mode Ta SawB Plate well ISamplg Date Time ngul A260 A280 260 280 260 Print ID ID Sawe and Print c ND 8000 Data c NND 8000 Data Reports Report Report 7 Section Tools amp Configuratiion ND 8000 Data Viewer vi File Configuration Data mE Canfigure Report Plots Report Sort Report Report Name Save Report Farmat Repart Full Mode lqnore yi Load Report Format Plate well Sa ID Save Report Load Report Print Report Testtype Configure Report
131. Default 30 Cancel 4 Default e e 99 s Administrator Administrator level 10 Acoount Management Modify User Administrator Change Password
132. E E 0 g 1903 1 1 1 1 1 1 220 250 300 350 40D 350 500 550 500 550 700 750 Waveleno CursorAbsorbance 0 267 Standard Curves BCA Lawry Bradford Pierce 660 nm NanoDrop 8000 Reference Standard 7 Standard 5 3 7 Section 3 Choose Standards Source Standards are TBduired Choose the Standard Curve S UTCB Load Standard New Standards Cu Measurements Cancel New Standards M
133. Column 8 Sample Configuration Palte Setup Mode Configuration Configuration Edit Na sy Help Detine Sample ID File Fnrmat 1SUTE Lnad Sarmple ID File Manual Sample ID Entre Sample ID RB uirg ple muto dance DIumns PromptClopse Data wiewer Measurement Lirmits Show Flate Surmmahy s Sample ID Required Sample ID Sample ID Sample Status Color Code Auto Advance Columns Sample Status Color Code Sample Position llluminator
134. dye dye Dye Chromophore List Editor DyeChrnrnnphnre List Namg 1 Cm nm qal ebpll nm 280 nm factor factor Insert lt od TOE 550 omen 00 on lt os 250Er5 550 60GExO ooo jon Alexa Fluor 488 710E 4 495 DOOE O OO0O ooo Alexa Fuor 546 104Er5 556 00OExO 000 000 eeded lt Algxa Fluar 555 T5OE 5 555 60GExO ono 000 Selected lt Alexa Fluar 594 730Er4 590 n0OErO ooo loo lt Alexa Fuor647 239Er5 650 000ErO ono 600 lt Alexa Fluar 660 132Er5 53 000ErO ono im lt Os 50Er5 581 0OQEtO 000 om lt os DES 575 900ExG on 000 es ED 220 oooern Q0O loo TE EE EE ee Ta Note predetined es are indicated with a diarmnnd and cannot be mndified 1 uL 1 5 2 UL 8
135. 3 dH2O dH20 0 5 M HCI 5 uL dH20 NanoDrop PR 1 1 PR 1 2 PR 1 3 30 4 4 PR 1 PR 1 1 UL dH20
136. Active Report Show Report Data Viewer Report DataViewer 3 4 Section 3 User User Guidance Display Box Units Edit List Units List Editor New Units Wyeight Based Malarity Based le Scaling from g L 0 00E 0 Delete Selected Update Selected ngrul Units with a diarmond symbol are protected amp
137. Enter Sample IDs Sample ID replicate Enter sample IDs Enter the Sample IDs yia the keyboard or barcode scanner The well position increments automatically with each entry Click nn a well to go to that well samples 96 To de select a well setthe replicates to 0 Positinn Al Replicates 1 Sample ID B C D E F G H DODDDDODDDDO Next Well Enter Cancel Section Sample ID List Format Sample ID File Format Sample ID Plate Map Row
138. 5 30 Section 5 Standard Methods Pierce 660nm _File Edit Configuration Standards Help Blank Re blank Show Repor User Default Date Time 9 9 2008 436 PM Plate ID Measurerment complete RETA All Active Un Of nm1 660 Unis ug ml wiewyUpdate Standards eg Aclve 1 A3 samlet 1 1 n001 50 0006 i B SampleID Fi EED a 660 0 001 0 000 Active 1 st 8 Sample 1 nm 1 abs 0 015 750 0 001 il SamplelD Fi BB 0015 87 45 Active 1 3 LT ample 1 nm1abs 0 050 4750 0002 am SampleID Pierce660 3 0 050 289 1 mctive 1 R 3 1 ample 1 nm 1 abs 0 093 amp 75 0 001 om Sample ID Pierce 660 660 0 093 541 9 Active 1 ample 1 mm1abs 0 122 750 0 003 3 SamplelD Fi BB 5 0122 708 5 A W OO0OOOOOOQ re 1 somes 1 miaslo7 AL am Bl O OOOOOOOQ I sweep pecseo Naso oi 1030 Aclve 1 ample 1 nm 1 abs 0 234 A750 0002 aaml El OOOOOOOOO SamplelD Perce 80 660 0234 NaN Active 1 ample 1 nm1abs 0300 750 0002 nl W O000000000 SepL Po es0 n30 NaN 200C0158 30 28 s A660 nm dye 660 nm 1 mm nm 1 nm 1 abs
139. 5 24 Lowry Standard Curves File Standards Help Print Page Usgr Default 90402008 2 15 PM Return to Sample Acguisition 2 0 0 CO158 0 37 B Units mgnl Measure Blank Measurements Table Standard maml Ave Abs Abs 1 Ahs 2 Abs 3 Abs 4 Abs 5 Reference B Standard 1 C Standard 2 D Standard 3 E Standard 4 F Standard 5 G Standard 5 H Standard 7 Double Click on any row to change the concentration or delete replicates On 1004 005 0002 1005 1004 055 0028 005 0065 0048 004 074 0083 005 mi30 0133 015 164 209 0214 026 6219 6225 022 Standard Curve Absorbance Spectra 0 3 33 ED TE 4 400 425 450 475 500 525 550 575 600 625 660 675 700 725 750 Wawelength s Section 5 Standard Methods 2 2 Standard linear interpolation polynomial fitting
140. Report Configuration Standard Plots s Save Report Load Report 6 Section Tools amp Configuratiion Save Report As Choose how to save the report Choose this option to be able to reload the Ea report into the Data viewer at a later date Choose this option to export a tab deliminited text file ofthe displayed Report Table suitable for import into Excel Expor Report Table Only Choose this option to export a tab deliminited text file of the splayed Repnrt amp Standards Tables suitable forimport into Excel Export Report amp Standards Tables Full Report Data Viewer Load Report Load Report User Preferences
141. Print JPG File Save Window jpg Escape ESC Escape Escape 2 3 8 Section Sample ID List Format 4 Sample ID Sample ID Single Sample Sample Loading Mode Plate Setup Select Sample Load X Lnad Sample ID Manual Sample ID File Entry Cancel Sample ID File NanoDrop 8000 Single Sample 8 Sample Sample ID Excel txt C ND 8000 Data Plate Files
142. 5 Blank Blank 0 Reference dye NanoDrop 8000 Blank 5 31 Section 5 Standard Methods Choose Standards Source Standards are rduired Choose the Standard Curve source Lnad Standard New Standards Enter Standards Curve Measurements Manually Cancel New Standards Measurements centr Do yau want to load the concentrations and curve type for gqenerating a standard curve from a prewiously sawed standard Curye file Yes Standard
143. A280 4706 260 280 1 85 260 230 219 4384 CI OOOOOOOOO ET cr es ctive Sample 8 260 8 DI OOOOOO ample 1 nm 1 abs 8 804 260 8 804 ngful ESOCESTOTTOTTTDOO SameD Mn A280 4710 260 280 1 87 260 230 219 _ 4402 FI OOOOOOOO Active 1 H3 Sample 1 nm1 abs 8 802 260 8 802 _ ul GOESTEOOOOOOOOD 5 ample ID nyul _ A280 4715 2 19 Hl OOOOOOO 7 25H 28H 1 87 260 230 13 200C0158 0 37 48 Sample Plate Map 3 6 Section 3 Sample ID Sample Plate Map Sample ID Sample Position llluminator 96 Sample Status Color Code Status Status Indicates hurmher nfrehlicates Indicates actual number of Speclfied in plate set up replicate measu
144. PR 1 DNA 55C NanoDrop 8000 DNA DNA Blank Blank Blank
145. pH William W Wilfinger Karol Mackey and Piotr Chomczynski1 Effect of pH and Ionic Strength on the Spectrophotometric Assessment of Nucleic Acid Purity pH BioTechniques 22 474 481 March 1997 260 nm 280 nm 260 280 1 nm 260 280 0 2 1 nm 2 260 280 0 4 DNA RNA 5 260 280 260 280
146. 13 Device Manager 2 NanoDrop Thermo Scientific NanoDrop Products NanoDrop 2000 NanoDrop 2000 2 USB 1 9 2 Section 8 Connection Error Connection Error There was an errorcommunicating with the instrument Try the following Check the USE cable and select Rietry lf Rietry fails disconnect the USB cable and then reconnect and select Retry again lf this dnes not snlye the problerm referto the Troubleshooting section of the User s klanual available from kain klenui USB USB PC
147. 7 3 Section Tools amp Configuratiion Plots Plots es Test Type e Date e Selected Plot 2 Selected Plot Legend Plots Sets 1 20 20 1 s Legend Ledend
148. Measurement Type Mesurement Type Measure Blank Reblank Measure Blank Blank Blank Reblank Blank 1 Microsoft Excel Nucleic Acid 2005 11 01 v3 2 ndj Read Onlyl File Edit View Insert Farmat Tools Data Window ACT Help AdobePDF Type aquestion For hi EE Fr ly y mT ow Co Madule INucleic cid _2 Path _3 Software 3 2 0 4 Firmware USB2HHH 2 41 3 ND3 5 SampleID UserID Date Time ngzul A250 A250 26072280 260230 Constant Cursor PosCursor abs340 raw Measurem 5 t 117172005 8 3B AM 0 0 Na NaNN 50 230 0 0 Blan Default 117172005 8 39 AM 3 51 0 07 0 03 2 42 2 12 50 230 0 033 0 Measure Default 117122005 8 39 AM 43 75 0 88 0 52 1 67 2 43 5H 230 0 36 0 008 Measure Default 1127172005 8 40 AM 393 73 7 87 4 27 1 85 2 35 5H 230 3 353 0 008 Measure _10 Default 117122005 8 41 AM 3704 14 74 08 39
149. Report configuration editor Selectthe report columnsto displasy and the nrdgr thatthe columns should appear Change the order by selecting and dragging a column header string tn he desired position Report columns Cursor Pos Cursor abs 340 raw kleasuremant Type Constant wv OK Report e Sort e Save Report Format nr8 Users Preferences Reports Select Default Report Format s Load Report Format s Print report
150. Standard Curve Absorbance Spectra Standard Curve Type Linear R squared 0 9929 5000 6o00 700 BOO 9000 10000 ugrrml i000 2000 3000 4000 Pierce 660 nm Pierce 660 nm 5 33 Section 5 Standard Methods Cell Culture NanoDrop 8000 1 mm 1 cm 10 Cell Cultures 250 nm 700 nm 600 nm
151. 30 40 NanoDrop PR 1 PR 1 www nanodrop com NanoDrop 8000 100 mg mL BSA 0 2 mm NanoDrop 8000 BSA 6 6 ro EN 96 replicates SD mg mL CV 0 15 5 mg mL 0 15 mg mL Purified BSA 0 15 mg ml 100 mg ml gt 5 mg mL 2 5 Configuration Measurement Limit 280 nm
152. DOAN Rn i ek 2 1 RN SN Ni 2 3 SS 3 1 3 1 DO EEE Ni i 3 2 ON 3 2 Single Sample 8 Sample i 3 3 Single Sample 8 Sample 3 3 Single Sample i 3 6 8 Sample i 3 6 3 7 Saimpble D 4 1 Sample ID Single Sample 4 1 Single Sample Configuration 4 1 Sample ID 8 Sample 4 2 8 Sample Configuration 4 4 Sample Position llluminator 4 5 IaidardIMGINOGSEE Es 5 1 5 1 5 2 5 2 5 2 WigoealeU 5 4 Protein A280 kk 5 5 5 5 SNP 5 5 IE AS 5 8 VE DOD RR 5 8 5 9 5 10 VS 5 12 i 5 12 UI 5
153. ManoDrop Dewices ManoDrop LED Position Indicatnr 4 MD B000 Peripheral Cnntrnl Cewice MD B000 Spectrophotometer etwnrk adapters PCMMCIA adapters 9 Pnrts Co LPTI 4 NanoDrop 5 USB 5 USB Found New Hardware Windows XP SP2 No not this time Found New Hardware Wizard 2 1 1
154. 1 mg7ml EI DODOO OOI sampeD BSAg3 F nm1abs 4832 Dye2abs NaN Due2uM NaN 7 091 F 8 1 OOOOOOOOOO Actve 1 H1 sample 2 Dye 1 abs 4 231 De1uM 28 21 mg ml SamplelD BSA cy3 M 1abs 4 785 NaN 7 110 OOOOOOOO F nm 1 abs Due2abs NaN Due 2 ukd a Active 1 D1 Sample 2 Dyelabs 4302 Dye 1 uM 28 68 mg ml e Type 75H nm 1 Sample ID BSA cy3 nm 1 abs 4971 Due2abs NaN Due2uM NaN 7 258 Active 1 M B1 _ DLL Sample 2 Dye 1 abs 4 275 Due 1uM 28 51 mag ml SamplelD BSAcu3 i nmlabs 4923 Dye2abs NaN De2uM NaN 7 340 clive 1 C1 Sample 2 Dye 1 abs 4327 Due1uM 28 85 Dye 1 Cy3 vy min SamplelD BSA cy3 2 nm1 abs 5013 Dye2abs NaN Due2uM NaN 7 315 Dye 2 None 340 Bichromatic lg active 1 E1 E1 Sample LE ample 2 Dyelabs 4295 Dywe1TuM 28 64 ml Sample ID BSA cy3 nmlabs 4970 Dye2abs NaN Due2uM NaN 7 238 1 12 2 0 0 CO200 0 09484 Sample Type Proteins amp Labels Protein A280 6 Sample Type
155. 5 Sample ID BC amp Al OOOOOOO Active BI OO OOOOOOOOQ 5ampelp BCA Active Sample ID BE amp Active OOOOOOOOOOQI IP mgzml 562 0 056 1 291 kk HH C1 Sample 1 nm1 abs 0048 750 0 025 mgiml A 552 0D 048 1 050 DT Sample 1 nm 1 abs 0062 750 0 023 mgiml 552 D0 052 1 498 a E1 Sample 1 nm1l abs 0068 750 0 029 mgiml A552 0 068 1 666 k F1 Sample 1 nm 1 abs 0067 750 0 022 mgrml 562 0 067 1 641 G1 5ample 1 nm 1 abs HUEE 75H 0030 md ml 562 0 066 1 506 H1 Sample 1 nm1 abs 0 074 z50 0 025 Gk mgiml 562 0 074 1 859 8 2 0 0 CO200 0 03 8 s A 562nm 562 nm Cu BCA 1 mm e nm1andnm 1 abs 1 mm mg ml Unit s Show Report 200
156. A280 BCA Cu BCA 562 nm 750 nm BCA CuSO4 Standard Bradford 2 uL
157. Narmalize Active 1 MK E1 Sample 1 nm1 abs 0 736 nm2abs 0002 _ Sample ID unknnwin 5 IE 1 ea 12 A ODOOOOOOOD Aclive 1 F1 Sample 1 nm1 abs 0733 nm 2 abs 0 000 BI OOOOOOOOO Sampelp unknown6 9TSTOTSTOTOTSTGTSTEGTG Active 1 G1 Sample 1 nm1 abs 0735 nm 2 abs 0001 OO OO OO OR SamplelD unknown Active 1 Sample ID unknawn 8 H1 Sample 1 nm1 abs 0 739 nm2abs 0 001 Hl OOOOOOOOOOO 200CPE100878 e Automatic Path Selection 2 2 1 25 1 0 mm 0 2 mm 0 2 mm 0 2 mm 1 0 mm Users Preference UV Vis
158. 5 7 5 Standards 7 7 Diagnostics and Utilities pt 7 8 kk 7 8 7 13 Account Management pp 7 13 User Preferences ki 7 15 8 1 8 1 Unrecognized DeviceS 4 8 1 Conneclion EO Ro SR RE 8 3 SIONalE MO NNN NN 8 4 ETORC SUE Se 8 5 EO SU 8 5 8 6 OFJL LAAI ka 8 7 i 8 8 POU SO RA bb iii 8 9 SE a 8 10 9 1 9 1 cas 9 1 Mui 9 2 9 2 i 9 2 9 2 9 2 DE FREE 10 1 10 1 Blank kk 10 1 lt 10 1 Ra 10 1 10 2 Section 7 1 Thermo Fisher Scientific NanoDrop W 8000 8 1 UL 220 750 nm 8
159. NanoDrop 8000 V2 3 User s Manual Thermo Fisher Scientific
160. mgrml SamplelD BSA A 280 1 264 1 836 340 nm Narmalizatinn Active 1 Sample 1 nm 1 abs 1 167 260 280 0 74 8 md ml SampleID BSA Ra A280 1 175 1 762 1 1 12 1 76 Al COCOOOOOOOOO00 Active 1 F1_ Sample 1 nm1 abs 1 235 260 280 074 ii BI OO OOOOOOOO sanpeip Bsa 280 1 243 1 864 Cl OOOOOOOOO SEE ctive DI OOOOOOOOOOO 5ample 1 nm 1 abs 1 186 250 28H 0 75 mg ml e 4290 1183 0 F TOOOOOOOOOOOD Active 1 HH Sample 1 nm1 abs 1 282 20 28H 0 75 GI OCOOOOOOOOOOO ce a ample ID 280 1 291 Hl OOOOOOOOOO 5 200C0200 0 09 52 Sample Type 6 Sample Type 8 Sample Type 280 nm 1 0 A 10 mm 0 1 1 mog mL Bovine Serum Albumin
161. 0221 0216 0219 0222 0221 0223 Acve HG Sondarde 020 0275 EL mini BCA Sdo Gove FT 0 01 0 20 mg ml Standard Curve Type Lneor Fsquered 0 9955 BCA BCA 5 21 Section 5 Standard Methods Protein Lowry Lowry Lowry BCA Bradford Lowry Lowry cupric sulfate Folin Ciocalteu 650 nm 405 nm
162. 2553 33 19 Aactive 1 B Sample 1 De 1 abs 0351 Dwe1pmaluL 23 37 ngjul SampleID Cy3 E5 nm1 abs 0117 Dwe2abs 0639 Due2pmaluL 25 58 34 86 Aclive 1 CP Sample 1 Dye 1 abs 0352 De1pmaluL 23 47 Sample ID Cw3 E5 nmlabs 0122 Dye2abs 0648 De2pmaluL 25 94 33 89 Active 1 D2 Sample 1 Dyelabs 0352 Dwe1pmaluL 23 45 Reil samplelD Cy NA m1abs 0124 Dye2abs 0638 De2pmoMuL 25 54 33 76 active 1 Sample 1 Dye 1 abs 0352 De1pmaoluL 23 49 ng ul Sample ID EC93E5 miabs 0120 Due2abs DEG40 Due2pmaluL 25 59 33 84 Active 1 FE Sample 1 Dye1 abs 0354 Dywe1pmoluL 23 57 Sample ID Co34C5 _ nmlabs 0131 Due2abs 0639 De2pmaluL 25 57 33 99 ctive 1 Sample 1 De 1 abs 0 351 Dye 1 pmaluL 23 39 ng ul SamplelD Cy37Cy5 mlabs 0118 Dye2abs 0637 Dwe2pmouL 2550 33 54 active 1 Sample 1 Dye 1 abs 0352 De1pmaluL 23 47 ng ul SampleID Cy3 E5 _ nm1abs 0123 Due2abs 0633 Due2pmoluL 25 34 33 72 20 0 CO158 0 46 8 A595nm 595 nm 1 mm e nm1andnm 1 abs
163. Check Maln Menu Calibration Check CF 8 CF 8 8 PCR 2 K CrzO NanoDrop 8000 Thermo Scientific NanoDrop 8000 Calibration Check 8 5 1 Calibration Check 1 PR 1 2 PR 1 30 3 4
164. 00c9 10000 0047 NaN NaNN NaN NaNN NaN Standard 2 Interp HU 0 029 100 00 0 04 1000 00 0 099 NaN Na Na Standard 3 Interp 000 0029 10000 0047 1000 00 0108 2000 00 0073 Na 7 7 Section Tools amp Configuratiion Microsoft Excel C WNND 8000 Data Reports Data Viewer Excel Diagnostics and Utilities Calibration Check Utilities and Diagnostics Utilities Diagnos Select Diagnostics or Calibration Check DiaqnnstiCs Calibration
165. 1 04E 5 0 12 Selected 1650 9 5 5 d 2 6564 jp Em exa Fluor 594 7 30E 4 590 000E 0 043 056 650 000E0 0m 132Ex5 663 nnOExO 000 9m 150E 5 561 000EXO ooo 1000 1 Agxa FIunr 547 2 3dE 5 Axa FIunr BB Cy3 5 Cy5 5 TS cn cn 250Ex5 DyLight 488 7 00E 4 0 15 DyLight 549 50E 5 0 08 DyLight 594 8 00E 4 595 000E 0 040 059 DyLight 633 1 70E 5 0 11 DyLight 649 2 50E 5 DyLight 680 1 40E 5 584 0 00E 0 10 14 0 13 Nate predetined des af indicated with a diamond and cannotbe modified 4 5 AlexaFluor555 SEA5 15 0 00E 0 4 5 Cm co co Section 8 8 Unrecognized Devices Instrument Peripheral Control Device or LED Position Indicator Not Found USB 1 USB 2 USB
166. 17 Load Report Format 7 16 Section Tools amp Configuratiion User Preferences File Help User Default Microarray Prateins amp Labels Pratein 280 General Settings Reports Data Exporting Nucleic Acids Uw Vis Auto Reporting Nucleic Acids Pratein A280 Select Custom Uv vVis Pratein BCA Report Format Microarrasy Protein Bradford Prateins amp Labels Protein Lowry Single Sample Cell Cultures Fratein Pierce 660 nm 8 Sample Default Reports Module Custom Report Standard Nucleic Acid report format nf8 MicroArray Standard MicroArray report format nf8 UV Vis Standard UV yis reporformatnfg _Pratein A 280 Standard Protein A 280 report frmatLnf8 Cgll Culture Standard Cell Culture repartfarm atnf8 Prateins amp Lahels Standard Prateins amp Lahels reportformatnfg BCA Protein Standard BCA Protein report forrmat nf8 radfard Standard Bradford repartfarmatnfB Lowry Standard Lowry report format nfg Pierce bbOnm Standard Pierce bbOnm report format nf8 Custom formulas nf8 Save amp Exit Cancel Auto Reporting Auto Reporting
167. Level 10 level 10 level 10 Administrator nanodrop level 10 level 5 Administrator level 10 Level 5 User Preference c md 8000 Data User Preference Default level 0
168. Oligo Calc Oligo amp Oligo Oligo Caluculator e nm1and nm 1abs 10 mm s A260 260 nm 10 mm 1 mm 10 0 2 mm 50 A280 280 nm
169. cannot be deleted Select and drag any unit to re order the units list NOTE the kal Wt must be known for the sample tyhe being measured to conwert between weight and Molarty based units Weight Based Molarity M Based Sample ID Sample ID Sample ID Sample ID Sample ID List Exit Exit 10 10
170. wy Standards 5 19 Section 5 Standard Methods es Standard 5 1 Standard Reference 2 Standard Standard cBCAProtein Standard Curves File Standerds Help User Deteult 4372007 2 43 PM Faturn to Semple Acquisition 6060 Teble Double Click on ony row to change he concentraton delete repliCelas Teml vesbs Abs 1 Abs 2 Abs3 Abs4 Aos5 Actve eo ooes 0007 097 0002 oo os Actve B Siondord 1 0045 0046 0044 0040 0046 0048 Acive Sengerdz 040 qo 0103 0099 0094 Bliank Acive D Sanderg3 0120 0123 Active E Swandgard4 100 9210 0211 0 Actve F SMendard 5 2 00 0355 0357 0354 0 593 AcCfve G Standerd 5 400 0 591 0 593 0 591 Acve H Sendsd7 800 9923 0921 0927 ji Stondord Curve Absormbanc
171. 1 Cy3 ngful Sample ID Cy3 E5 nmlabs H122 Due2ahs 0648 De2pmoluL 25 94 33 89 Dye 2 Cy5 Y se Active 1 D 5ample 1 Due 1 abs 0352 Dywe1l pmoluL 23 45 ngAl samplelD Cy3C5 LL nm1abs 0124 De2abs 0638 Dye2pmaluL 2554 33 76 Act ctive Dyelabs 0352 Dowe1pmoluL 23 49 ngful Cy3 E5 2 12 sample Dye2abs HB4H Dye2pmolAuL 25 53 33 84 Al OOOOOOOOO Aclive 1 Dye1 abs 0354 Dye1pmolul 2357 ua BI OOOOOOOO 5ampelp Do3 C56 Dye 2abs 0639 De2pmouL 2557 33 99 CI OOOOOOOO ET ctive D 1 1 De 1 abs 0 351 De 1 pmoluL 23 3 ngful 6 Dye2abs 0637 Dye2pmoluL 2550 33 54 F 8 SOOOOOOOOOO active 1 Dye 1 abs 0352 Dwe1pmauL 23 47 GI OOOOOOOOOO TT 99 ample ory 25 34 OOO OO O O O O Dye 2 abs 0633 Dwe2pmalzuL JJ 7 20 0 CO158 0 46 8 Sample Type dsDNA DNA 50 RNA RNA 40 DNA ssDNA 33 Other Other 15 150 MicroArray 3 DNA 50 RN
172. 10 00 es nm1and nm1abs A280 10mm Path 280 nm 10 mm 6 Section 5 Standard Methods s A260 280 260 nm 280 nm s Concentration mg ml 280 nm Units mg mL Show Report 200 340 nm Section 5 Standard Methods MicroArray DNA
173. 12 Proteins amp Labels 5 14 0pm2 lt 5 UP 5 14 6 RNN 5 15 ProteinBCA Sk 4 5 17 9 UM 5 17 BCA 5 18 EOWRE 5 22 bn M FU 5 22 Lowry 5 23 Protein Bradford 5 26 KK 5 26 Bradford LL 5 27 Protein Pierce 660nm 5 30 5 30 10 Pierce 660 nm 5 31 Cell Culture Oo 5 34 i 5 35 ED TE 5 35 User MethodS EEE 6 1 Melhod Ed ER A 6 1 OA 6 1 JoOOR 6 4 kkk 6 4 Tools amp Configuratiion ee 7 1 i i 7 1 IVIS 7 2 In RI 7 2 PI R Rc bb biz 7 4 019
174. 3 Sampe57 Sampe55 Sampe73 Sampegl Sampe83 ample 2 Sample 3 Sample11 Sample19 Sample27 Sample 35 Sample43 Sample 51 Sample 3 Samge67 Sampe75 Sampe83 Sampe91 Sample 4 Sample 5 Sample 5 Sample 7 Sample TT Continue Cancel Sample ID Continue Sample ID Sample ID 96 Select Plate Set amp Confirm Sample Loading The selected Sample ID file has more than 96 samples Selectthe desired Plate Set frnm the file PlateSet 1 Sampl ID File CAND 8000 DataiPlate flest3 plates Data by rows bt Sample well Position Assignments 1 2 3 4 5 5 7 8 3 10 11 12 Define Sample ID File Format You may chooseto skip sample columns in this Plate Set by choosing a tart Column otherthan 1 default Start Column 1 Plate Set Sample Map Define Sample ID File Format Manual Entry
175. 69 A 260 8 769 ngful Bl SOOOOODOOOO T anelp 40ngu A280 4706 260 280 185 260 230 219 4384 CI OOOOOOOO 1 cm ctive Sample D O 5 ample 1 hm1abs 8 804 260 8 804 ngful ESOSTOSTSODTOSTOTST SameD TngM A amp 280 4710 260 280 1 87 260 230 219 24402 FI OOOOOOOO Active 1 Sample 1 nm1abs 8802 amp A 260 8 802 leee OOO EO ample ngru 28H 4716 2 13 IS OOOOOOOOO 260 280 1 87 260 230 200C0158 0 37 484 Section 5 Standard Methods s Sample Type dsDNA DNA 50 RNA RNA 40 DNA ssDNA 33 Oligo Calc Other DNA 50 Other 15 150 3 DNA 50 RNA 40 ssDNA 33 Sample Type
176. 80 A AZGD 495 280 Edit Farmulas Cancgal Edit method parameters Step 5nf5 Name kiethod name Alexa 488 Prntected Description fluorescent dye optional 6 3 Section 6 User Methods Default Edit Selected
177. 84 1 86 2 24 5H 230 33 75 0 038 Measure C IND 8000 Data gt User name gt BCA Protein Lowry Bradford Cell Culture MicroArray Nucleic Acid Protein A 280 Proteins amp Labels UV Vis Address CAND 8000 DataDeFaulUMicroarray vy eo Folders X Name Size Type Date Modified EE ND 8000 Data Microarray 2007 03 21 vl 14KB NDS File 312112007 4 02 PM Administrator Microarray 2007 03 23 v1 18KB NDS File 3 23 2007 8 57 AM a Custom report Formats Default Cell Culture Oe EE Nucleic Acid 7 1 Section Tools amp Configuratiion Tools amp Configuratiion User Preference Data Exporting On Automatic Data Export Data Export
178. A 40 ssDNA 33 Sample Type nm 1and nm1abs Dye 1 dye Dye 2 dye Dye 1 2 abs dye 10 mm Concentration ng ul 260 nm Units ng uL Show Report 200
179. Folder Save and Exit User Pereference PC Data Viewer Data Viewer DataViewer Show Report PC NanoDrop 8000 Data Viewer
180. Sample 1 nm 1 abs 0460 A 600 0 076 Sample ID Active 1 _E1 Sample 1 nm 1 abs 0 459 a 600 0 075 Sample ID 1 1 12 Al COOOOOOOOO O00 Active 1 F1 Sample 1 nm 1 abs 0 470 A 600 0 084 B OOOOOOO OO sampep CIO eOOOOOOOOOOOO ctive Sample 8 D OO ample 1 hm 1 abs 0 467 600 0 079 ee FI OCOOOOOOOOOOO Active 1 H1 Sample 1 hm1abs 0 471 Aa BOD 0 086 GOOOOOOOOOOO 1 ample HI WOODOD 200CPE1008 87 e 600nm Absorbance 1 1 mm nm 1 s Show Report 200 5 34 Section 5 Standard Methods 1 UL
181. ate ID Autnmatic Path Selection Analysis Wawelength 260 Factor 5000 Mol Weight gimol 0000 1 1 12 OOOOOOOOOO0O OOOO OO OO OO OOOO OO OO a nn OOOOOOOOOOO Make new BLANK Measurement Ne All Active On Of nm1 260 Units ngAul Active 1 Sample 1 nm1 abs 1 118 260 280 1 86 nail SamplelD Sample1 A 260 1 118 A 280 0602 558 8 Active 1 Sample 1 nm 1 abs 1 136 2EH 28H 1 80 ng ul Sample ID Sample 2 A 260 1 136 A 280 0 633 568 2 Active 1 WI 1 Sample 1 nm1 abs 1 158 260 280 1 79 SamplelD Sample3 A 260 1 158 A 280 0648 573 1 ctive 1 Sample 1 nm1 abs 1 131 2604280 1 84 ngf ul SamplelD Sample4 A 260 1 131 A 280 0613 565 6 Aclve 1 Sample 1 nm 1 abs 1 069 260 280 1 76 ET SamplelID Sample5 A 260 1 069 A 280 0 608 534 3 Active 1 Sample 1 nm1 abs 1 155 260 280 1 83 ngjul SamplelD Sample6 A260 1 155 A 280 0631 577 4 Active 1 LU EL Sample 1 nm1 abs 1 113 260 280 1 82 ng ul SamplelD Sample7 A 260 1 119 A280 0 614 559 6 Active 1 LT TI Sample 1 nm 1 abs 1 073 260 280 1 77 5 SamplelD SampleB A 260 1 073 A280 0608 536 7 Single Sample 8 Sample
182. ay 2 UL e IMicrobial cell Suspensions 1 2 UL 1 2 0 2 2 uL 8 NanoDrop 8000
183. e Spectre ch A Pa E Ch B 08 AL ch C Ch D NU 0 5 7 a ww Ch E 1 Ch F NA 4 4 2 pe 4 A 1 rh Ch G 2 gt Ch HH a se 450 460 500 520 540 560 580 600 620 640 550 680 700 720 750 Waveleng 2 2 Standard linear interpolation polynomial fitting BCA Protejn Standard Curves File Standards Help Detault 32007 3 26 PM Retum to Sample Acgussmon Print Page 10T 2 COO 4 0 18 28v9 j Measurements Teble Double Click on any row to cha
184. easurements Measurements Tahle Double Click n ntratinn nr dglete rehlicates Do ynu want tn lnad the concentrations and Curve type for qenerating a standard Curve from a prewiously sawed standard curve file Yes Standard Standards User s Manual Help PDF Start gt Programs gt NanoDrop gt ND 8000 Print WindoWw File CtrlrP
185. ectra 0 2 133 3 1 gt TS lt 0 0 1 1 1 1 TRY RC 1 i 1 1 1 550 560 580 p00 620 640 660 680 zDD 20 zd0 750 wawvelength 2 2 Standard linear interpolation polynomial fitting Pierce 660nm Standard Curves File Standards Help Raturn to Sample Acguisition 200C0158 0 30 284 User Default 979 2008 402 PM Units ud ml Blank kMeasurements Table Standard Reference Standard 1 Standard 2 Double Click on any row to change the concentration nr delete replicates ufml AveAbs Abs 1 Abs 2 Abs 3 Abs 4 Abs 5 im ia am non no oo om 15 1 500 2000 ug mL
186. ein Bradford Configure Data L Exporforkodule DOwry Pierce bbOnm Custom Configure Data Export for Module Nucleic Acid Data Export Configuration Selectthe data export fields and the order that the fields shnuld appear Change the order by selecting and dragging an iterm to the desired position Optinallw enable the export of the raw ahsnrhance data range and log Interwal Export Fields abs Spectrum Export shawAll Sample ID Disabled Lisgr ID Date 2 min 220 Time li 2260 280 2607280 260 230 Constant Cursor Pos Cursor abs User Preference Save amp Exit 1 7
187. ference 0 00 Standards File Edit Configuration EUTisUsE Help Lnad frnm file Measure Blank Wiew or Measure Standards Show Report s Standard Active Inactive Standard Standard Edit Standard Absorbance Replicate Standard Edit one standard Stan ard Standard 4 ud ml 1000 0 Awerage absorbance 0 124 Abs Replicates Delete SGlactad 0 123 EE 0 129 0 123 Reset Standard 0 125 wy Standards
188. ion llluminator Plate Summary Plate Summary Sample Position lluminator Plate Results Summary Plate ID 4q25 ng ul Plate Results hu 1 2 3 4 5 6 8 9 10 11 12 458 2 459 6 460 7 4603 4600 4603 458 9 4580 4595 458 6 4588 4585 4609 4609 4612 4596 4606 4659 4610 4598 4612 4611 461 6 456 9 4588 4565 4577 4559 4578 4589 4583 4582 E 4615 4577 4590 4592 4587 4586 4590 4601 4602 4595 4611 4513 4598 450n 457 4595 457 1 4585 4583 4586 4542 4593 4505 453 2 4597 4595 4575 4585 4565 4568 4560 4593 4575 4583 4585 4595 45356 461 4 4622 4603 4616 4598 458B6 4648 4616 4623 4513 4517 4508 H 4586 4599 4559 4586 457 4 4590 4608 4587 4585 4605 4606 450 2 Cell Color Codg Click on any cellto selectide selectthat cell for repeat measurements White inside limits Red nutsidg limits Light green inside limits amp selected Repeat Selected Close Window Dark green nutside limits amp selected 4 6 Section 5 Standard Methods 5 Standard Methods
189. m wavelength fnm 750 Report 495 wwawelengths 4d00 750 Edit Report wwawelengths mm Report Wavelengths 4 3 Baseline Correction Type 6 2 Section 6 User Methods Edit method parameters Step 3 of 5 Baseline Baseline Correction Type Slope Alinear baseline correction from BaselineCorectionwaveledth 1 _ to Baseline Correction Baseline Correction Wawelength 1 hmi 400 Wawelength 2 is substracted from the raw absorbance spectrum Baseline Correction Wawelength 2 nm 750 Cancgal 4 2 Edit method parameters Step 4 nf5 Fnrmula You may hawe up to 2 user defined formulas calculated for this method Select one nf the previnusly defined formulas from Availahle Formulas or first select Edit List to detine a new forrmula Avallahle Fnrmulas Farmulas used with this Method 2maximurm 260 280 Name Formula 260 230 gt gt gt 495 2
190. mo Scientific NanoDrop Product Ports COM amp LPT mw 7 Next 8 Advanced Next 9 Thermo Scientific NanoDrop Next 10 Have Disk Install from Dick 11 Browse ocg Disk C Program Files x86 Thermo NanoDrop 2000 ndusb32 6 12 Next code10 This device cannot start Finish
191. n Co wl Co LIE RL TII ml ml ml ml IT ol ol ol on ob Nate predetined es are indicated with a diarnond and cannot be moditied Bradford 2 uL 8
192. nge he concentretion or delete replicates Standard _mgfml Ave Abs Abs 1 Abs 2 Abs 3 _Abs Active B Standard 1 0046 0044 0040 0046 0 Active C Standard 2 0103 0099 0094 0100 ctive D Siandard 3 012 23 Active Standard 4 ma Active Actve mm BCA 0 2 8 0 mg ml Standard Curve Absorbance Spectre Sandard Curve Type Pokmomiel 2nd prder w a Py a a US Rsquered 0 9984 1 T 1 H i 0Ex0 2 0E 0 3 0E H 40E 0 5 0E 0 5 QE D 70E D 8 0E 0 mgrml 5 20 Section 5 Standard Methods BCA Protein Standard Curves Eile Standards Help Print Pege Deteult 32007 2 37 PM Rietum to Semple Acgussmon 10T2C0014016 2B 8 Measurements Teble Double Click on any row to change he Conceniration nr delete replicotes Standard moml AveAbs Abs 1 Abs 2 Abs 3 Abs 4 Abs5 Acive A Reference 000 0002 0004 0006 0003 0004 0005 B Siandard1 001 0918 0017 0015 0019 0019 6022 Acive C Standord2 003 0038 0035 0036 0038 0036 0043 mecive Tp Senesd3 E Sanderd4 010 0134 0132 0133 0134 0136 0135 Acive FStanderd5 015
193. ns Serif font 8 1 2 3 4 OK www scrum net co jp www nanodrop com USB USB User Preference Automatic Export Report PC USB
194. ple Position llluminator 96 Sample Position lluminator Sample Status Color Code Plate Configuration Sample Position llluminator Plate Result Summary Plate Review Auto Advance Columns Plate Result Summary Configuration Show Sample Summary 4 5 Section Sample ID List Format Sample Posit
195. rements made 0 Protein A 280 Fie Ed Confiqurahon Measure Blank Feslank Plate ID Sample Type _ 1Abss1momL Al Active C O Units maml Acive 1 A Al_ Sample 2 mn 1 abs 1016 2607280 1512 um Sample ID lest A280 1016 8 2 Sample Sample ID replicate Sample replicate nm
196. s Standard Curve Absorbance SpeCWa 0 3 er Bradford 15 100 ug mL a 0 0 1 LM L 0 0E 0 1 0E 3 20E 3 3 0E 3 40E 3 5 0E 3 5 0E 3 7 0E 3 8 0E 3 urml Bradford Bradford 5 29 Section 5 Standard Methods Protein Pierce 660nm Thermo Scientific Pierce 660 nm Protein Assay Bradford
197. s nccurrin try cleaning both measurement pedestals with water and then wiping each surFace 50 times aaressiyely with a dry lab wipe IF calumn breakage nersisits use a larger sample size 1 5 2 ul 3 One or both of the measurement naths is out of calibratinn IF this warning persists and the ahaye remedies do not salye the problem cantact NanoDrop Technalanies or yur distributor OPEN USERS MANUAL 1 mm 0 2 mm 20 Bradford 1 UL
198. snrhance 0 120 Abs Rehlicates Delete Selected 3 Reset Standard ww Cx Ce Standards s Standard 5 1 Standard Reference 2 Standard Standard 5 32 Pierce 660nm Standard Curves File Standards Hglp Units ugiml laas Measure Blank urements Table Default rd 2008 403 PM Double Click on any row to change the concentration or delete replicates Standard Referencg Standard 1 Standard 2 Standard 3 Standard 4 Standard 5 Standard 6 ugirml Awe Abs ABs 1 Abs 2 Abs 3 Abs4 Abs 5 on 0 1 an ao oo 0000 2s00 0046 na 005 00 no6 0047 0120 0120 0m6 0m9 02110923 Return to Sample Acquisition 2 0 0 CO158 0 30 284 Section 5 Standard Methods Standard 7 Standard Curve Absorbance Sp
199. tinn 3000 Continue Cancel e Show Plate Summary Sample Status Color Code 96 Sample ID Configuratton Manual Plate Set up Configuration Sample ID Required ID Sample ID Sam
200. ws 7 32 bit 64 bit Blank NanoDrop 8000 Blank Blank log Blank Blank A c A E b c A A E liter mol cm b cm c Dye MicroArray dye
201. xa 488 dye Edit methnd parameters Step 1 nf5 kleasurement Type The concentration is calculated by dividind the absorbance A Tatthe Analysis wawelength by Chramaphnre Alexa Fluor 488 Extinctinn Coefficient times the _ path lengthi Bath the nalysis _ nm andthe Extinctinn Coefficient Extinctinn Coeff limol cmy 7 100E 4 are redquired parameters The Eoncentration will be returned in lthe sglacted Units For weight Basgd units a Molecular Weight IS required Quantificatinn Mode c A bxExt Coeff ww analysis Wawvelength hm 495 Units mM v ol Weight gimal 0 000E 0 Cancel Next Wavelengths Edit method parameters Step 2 of 5 Wawelengths The absorbance at all wawelengths between the Plot MIn Wawelength and Flat Max Wavelengths are autnmatically archived for aach measurerment Use the Repor Wawelengths listto define wawelengths of particular interest for tabular absorbance displasy during acquisition and autnmatic Inclusion in reports The Wavelengths listis pre populated with the nalysis and Baseline Correction Wawelengths Click on Edit Wawelengths to change weawelengths or dafing additional wawelengths Minimum Wyavelength Inrm 220 Maximu
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