"取扱説明書"
Contents
1. ae DN A Aisha 74 Campylobacter OO Cco z C 7 77 SG PCR Cejuni Cco7 C7e 7z227 Cco77 PFGE Cco7 DNA 9 56 1 6 C coli 12. 2 3 5 C gzzzz DNA 9 56 1 Cco77 1 17 1 C j7ejuni 3 4 CC 7eg7zZz7 DNA SG PCR C col C jejuni 2 Cco7 C7e7g777 SG PCR SYBR Green PCR 2 Cco7 C jejunt Cco77 C jejuni
3. 17 1 M C 7e7g727 i C co7 M 1 2 3 4 5 M M 1 1 2 2 3 4 5 M Sma 1 zz 2 PFGE 3 9 SG PCR PCR C co 6 is 1 2004 6 11 4 8 7 0 2 5 C jeiuni 2 2004 6 12 13 9 30 7 0 C jeiuni 3 2004 6 17 31 41 7 0 4 2004 11 5 7 5 5 0 0 es a C jejuni 5 2005 10 2 6 39 94 6 1 6 2006 12 22
4. 12 12 7 0 MM 7 2007 11 29 8 13 7 0 2 8 2008 3 28 2 7 4 0 uni i C jeiuni 9 2010 7 31 10 13 13 0 7 Cco z C 7 727 7 Cco7 7e7z27 PCR 1 IASR 31 1 2010 2 Fukushima H et al Int J Microbiol pii 864817 2010 3 ul 4 5 6 7 8 18 21 40 2003 Aisha A A et al Med Microbiol 56 1350 2007 21 2 151 2004 27 40 2009 Suzuki H et al J Vet Med Sci 7 3 255 2009
5. 52 2010 PCR Cjejuni Cco7 1 Campylobacter 90 Campylobacter jejuni C jejuni Campylobacter co7 C coli Cco77 Cg7 7 o0gc7e 22 10 9 C 7Z727 PCR DNA C j7ejuni C co77 Cco 7 2 2 1 10 1
6. 10 1 9 10 4 5 9 5 4 9 2 2 1 2 2 7 10 12 1 CCDA 42 48 2 3 DNA QIAampDNA Stool Mini kit Qiagen ISOFECAL Beads Beating Nippon gene 2 D
7. 2 6 PFGE C coli Sma I ZI CHEF DRTI BIORAD 0 5X TBE buffer 1 6 0V cm 6 8 38 4 14 19 2 7 C7ezzz7 C coli DNA 2004 2010 9 56 2 5 C co 7 cez SYBR Green POR 3 3 1 1 2 2 7 6 Campylobacter 7 1 Salmonella sp 07 C27 y o02c e FE C jejuni Tm11 4 lt 3nnn 5 zom C co7 Im 4 18 G60 51 62 63 64 65 56 67 58 69 70 71 72 73 74 75 76 77 78 79
8. 2 6 SYBR Green PCR 1 2 2 C co7 C 7 zz7 Cco77 C jejuni PCR C j7ejuni Cco7 C 7 zz77 Cco7 C co7 1 FTID T LUT 1 SG PCR 1 SYBR Green PCR C jejuni PCR ceuE Pr 9 C jejuni a C Jjejuni C jejuni C coli C coli 1 C coli 2 C coli Cejuni Cco7 3 4 PFGE C co77 5 2 3 Sg I pgI
9. Cco7 C 7g7z27 SYBR Green PCR C e7zz777 C jejuni 2 Cjejuni Cjeiuni CC 7 7Z77 Cjejuni T C coli C coli 1 C coli 2 8 C coli Cejuni C coli CC co 7 C jejuni Campylobacter CCDA my
10. BD 81 82 83 84 85 86 87 88 89 90 lemperature IAC C jejuni 3 2 Multiplex SYBR Green PCR SG PCR 2 DNA 24 B ceuE C 7e777 1 Tm1l 73 95 Tm2 77 40 2 Tm1 74 14 Tm2 77 39 C coli C jejuni Tm 1 ceuE C jejuni single PCR 2 2 1 2gs 4 FemB 3 3 PCR 2 2 C7ez777
11. NA 2 4 Multiplex SYBR Green PCR SG PCR DNA 24 Fukushima 2 DC DAC Yersinia ruckeri JCM15110 DNA IAC 3 8 PCR PCR PCR Tm Tm PCR IAC PCR PCR Thermal Cycler Dice Real Time System TP800 Takara SYBR Premix Dimer Eraser Takara PCR Fukushima ECIEC EPEC EHEC ETEC EAEC DAEC Shigella spp Salmonella spp Y enterocolitica yY pseudotuberculo
12. sis P alcalifaciens C 7677722 C coli V cholerae TRH V barahaemolyticus A Hydrophila S aureus B cereus Cperfringens L monocytogenes TDH V barahaemolyticus P shigelloides PCR 2 2 Cjejunt 6 2 CCDA 42 48 DNA Campylobacter Fukushima C jejuni AB F CTGAATTTGATACCTTAAGTGCAGC and AB R AGGCACGCCTAAACCTATAGCT 2 5 86bp Tm77 7 0 96 Cco 7 cezZ ceuE For CAAGTACTGCAATAAAAACTAGC ACTACG and ceuE Rev AGCTATCACCCTCAT CACTCATACTAATAG 72bp Tm73 7 0 43 SYBR Green PCR 25 PCR
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