DNA すいすい-R
Contents
1. TE PCR DNA DNA DNA DNA2. DNA PCR DNA I DNA R 800 ul 1 000 1
3. 2 9 2 B201 029 852 9351 FAX 020 4623 5611 E mail info rizo cojp http rizo co jp Copyright e2009 RIZO Inc All Right Reserved
4. 4 5 7O DNA 18S rRNA 1 131 bp PCR 1 0 Agarose Mi Marker 10O base pair ladder 1 5 2 6 3 7 4 50 mg DNA PCR Template DNAY 1 6 ul 10XBuffer 3 dNTP mixture 2 0mM each 3 primer 4 pmol each 1 3 Tag 5units 1 O 25 HzO Total 30 gl DNA 2 120 Stratagene Paq5OOO PCR 95C 2 min 94C 35 sec 55C 30 sec 35 cycles 72C 75 sec 72C 7 min DNA NFN R
5. 1 6 gp 1 3 1 v v 70 TE 10 mM Tris HCL 1 mM EDTA pH 8 0 pH 8 O 1 1 25 24 1 SIGMA Cat No P2069 7 3
6. CN 1 5 ml 1 000 20O 5m DNA DNA 1 4C DNA 1 720 ul DNA 8O 1 10 11 12 13 1 5 ml 4OO ul DNA 10 50 mg 33 400 DNA
7. 1 1 V V 50O 15 OOO ropm 20 25C 10 6OO 4 15ml 200 gl 1 3 15 OOO rpm 10 6OO 1 15ml 300 gl O5 15 OOO rpm 10 7O 1 000 1 15 OOO rom 4 10 50 100 1 TE PCR 5 MA R DNA 6 1
8. DNA DS OOOSN DNA lt Ver 1 1 RIZO Inc DNA 1 NN 9 FR Op MN MN MN O1 DNA DNA PCR DNA 85 mL 110 2 1OmL Xx 1 4C
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