1 取扱説明書 Table 1. Characteristics of Cellufine Formyl
Contents
1. 1200 1000 800 600 400 Flow rate cm h 200 0 0 0 1 0 2 0 3 0 4 pressure MPa Fig 4 Flow rate of Cellufine Formyl empty Cellufine Formy eSepharose 4B INO11 Ver4 1 2013 471 Ccllulin 4 pH 1 1 15mg mLgel 20mg mL2. CNBr NHS CNBr CNBr 1 4B 1
3. 15mg mLgel 1009 20mgmL 2 pH 2 pH pH pH 3 3 10mgmLgel 2 4 4
4. oo OO PP oO Mm Table 1 Characteristics of Cellufine Formyl Base gel porous cellulose spherical beads oo e520um oo Activatedgroup_________Fomyl aldehyde _ Fomylgroupconc 10zmoMmLeel uo Delivery conditon 02M Acetate bufferpH3 0 contamme 001 2 2 dithio bis pyridine l oxide 2 1 NaCNBH CH iNBH NaBH PreincuDation H 0 0 OH Reducing agent H ee Oc HN IA lt OR OR H H0 H HH Cellufine Formyl Ligand Schiff base Ligand immobilized Cellufine INO11 Ver4 1 2013 471 1 Celluline 3 1 I 1 SCBH CAS 25895 60 7
5. 40 509 4
6. Sodjum cyano borohydride NaCNBH TMAB CAS 229 Trimethylamjneborane 1396 CHsjNBH5 1 lt 2 TMAB SBH CAS 16940 66 2 TMAB Sodiumborohydnde NaBHL S S SCBH SBH 3 9 IL 1 pH4 11 0 2M
7. 4 lt 1 5 25 coupling mg ml ligand coupling efficiency 60 100 50 ee oOo 9 80 O 40 3 Le a Rd L 60 op 2 a oD 1 hn s EE a gt Ra 40 9 20 O Ls 10 7 9 0 0 0 20 40 60 80 100 ligand mg ml Fig 1 The effect of the concentration of ligand in the coupling reaction O h Glo Coupling h Gilo Coupling ratio HAS Coupling HAS Coupling ratio 100 a O O O oo 3 O 80 60 O 40 hGlobulin _ _ HAS 20 0 Fig 2 The effect of pH of coupling buffer on the amount of ligand coupled to the gel 5 10 reaction time H 15 20 Fig3 The relation between the time for coupling reaction and the quantity of coupled protein C h 7 Globulin 30 C HAS 30 C hy Globulin 4C A HAS 4C
8. HEPES 02M Tms HCT pH70 1M slycme ethylester 1M ethanolamine 02M pH70 2 1ml I 509 2ml 2ml 15 O 7 0 8g 1ml I 1ml 1 lt 2 1 2 7mg 2 lt 8 5 10mg
9. 50 100mg ml 0 1ml 20m 2ml 7mg 2 SBH oO INO11 Ver4 1 2013 4 1 2 Celluline 20ml 1mL 1mL
10. INO11 Ver4 1 2013 471 ligand coupling efficiency 5 SCBH SCBH MSDS SCBH 1 1 1 SCBH 2 N a OH 3 24 2 1 SCBH 2 NaOH 3 II 1 676 944 324 19853 19854 JNC 100 8105 2
11. 0 7 0 8g wet g 2 1 2ml 4 3 1 2 SCBH 5 10mg 2 16 20ml 1 2ml 4 2 20ml I
12. 1 03 3243 6150 FAX 03 3243 6219 E mail cellufine jnc corp co jp URL http Www jnc corD co jp fine jp cellufine INO11 Ver4 1 2013 4 1 5
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