清浄度検査キット(ふき取り) - キッコーマン バイオケミファ
Contents
1. PD 30 PD 20 1 2 Pen 2 B 20 lt 302. 20150410T 20160709 Pen Pen 40 T 105 0003 2 1 1 Tel 03 5521 5490 Fax 03 5521 5498 E mail biochemifa mail kikkoman coJp http biochemifa kikkoman cojp 2011 Kikkoman Corp 20150301 Pen Pen 40 2 20 lt 30
3. JEP a J Pen F Q a Pen ii 4 2011 Kikkoman Corp 20150301 kikkoma ATP AMP Hygiene Monitoring test kit Swab Test LuciPac Pen Instruction Manual CAUTION LuciPac Pen Product code 6033 1 1 Do not attempt to drink the kit reagent or touch it w
4. PPDK ATP AMP Mg PPDK AMP E OUD 2 Mg Os CO gt 1 ATP AMP PPDK ATP ATP F ATP
5. ATP ATP AMP Pen 20 5 100 Pen 40 20 2 40 1 2
6. 2 Pen Pen 2 4 Pen Pen 40 2 1 2 3 1 A 2 C
7. 4 2011 Kikkoman Corp 20150301 RE RE
8. 1 2 B 3 KIOWA Ea BE Er RLU Plt 3 mit SHO wae IER 3 RLU elas 1 2 200 400 500 1 000 1 500 3 000
9. Pen 2011 Kikkoman Corp 20150301 2 20 30 35 C 2 Pp 20 g gee OFF E OFF 1 PD 30 Pen P
10. 37 3 1 2 8 25 14 30 5 2 20 2 8 2 3
11. Raw materials Polypropylene Structural parts Swab stick orange part Main body casing Swab shaft Polypropylene Polypropylene Polypropylene Polypropylene Aluminum Polyethylene Polyethylene terephthalate Polyethylene Container for releasing reagent Measurement tube Aluminum bag with dehumidifying function Outer bag Precautions for Handling Be careful not to let the reagents or other substances in the kit get into your mouth or eyes or onto bare hands before or after use Rinse your mouth out thoroughly with water if any of the substances get into your mouth rinse off your skin with copious amounts of water if any get onto your skin and rinse out affected eyes thoroughly with copious amounts of water shouldit getinto youreyes andimmediately contacta physician for advice and follow the instructions given 2 Exercise enough caution when storing and disposing of the kit and its reagents to ensure that none of the substances become mixed in with food and other products Be careful not to get fingers caught when inserting the swab stick into the main body casing Please make sure to store this kit and its parts out of the reach of young children Note that the releasing reagent used in this kit contains cationic surfactants benzalkonium chloride 3 Take precautions when disposing of this kit after use to ensure that such substances do not become mixed in with food products at
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13. food production centers and similar facilities 3 Benzalkonium chloride is a disinfectant and antiseptic commonly used in hand and finger sterilizer solutions Storage 1 Kit storage Kits are to be stored at a low temperature 2 C to 8 C 35 6 F to 46 4 F for long term storage The kit can be stored below 25 C 77 F for up to 14 days or below 30 C 86 F for up to 5days before opening an aluminum bag without any adverse effect on the long term stability Do not freeze the kit 2 We recommend that you use all 20 swab devices in a single bag at one time after opening an aluminum bag If you have leftover swab devices that you must store after opening a bag be sure to store them at the recommended low temperature 2 C to 8 C 35 6 F to 46 4 F and use them within two weeks from when the bag is opened 3 Expiry date Printed on the aluminum bag Manufacturer LuciPac Pen Warranty Kikkoman Biochemifa Company warrants the products in this kit to have a certain level of quality This warranty guarantees that Kikkoman Biochemifa Company shall replace defective products should any be found This warranty does not provide any other guarantees Kikkoman Biochemifa Company shall not be liable for any damages including special or consequential damages or expenses arising directly or indirectly from the use of this product Symbols Used in the Packaging and Labeling of this product Symbol for temperature l
14. the measurement test on a dry subject first moisten the cotton swab or the test subject with tap water 2 3 Use it to swab the test subject with constant pressure as much as possible Under suitable pressure the swab shaft will slightly bend as illustrated in the panel 3 of page 8 4 Return the swab stick to the the main body casing and push it all the way into the the main body casing Hold firmly onto the LuciPac Pen casing and shake it Allow the leftover luminescent reagent to thoroughly dissolve Insert the LuciPac Pen into the Lumitester to measure the results 2 Regular tap water may contain slight amount of ATP and AMP We recommend that you wash or otherwise clean off the faucet in such cases and allow the water to run for a while before collecting any for use Refer to Direction for LuciPac Pen on page 8 for further details 2 Handling of data Benchmark levels 1 2 are set that are used to determine whether cleanliness levels are at acceptable levels or not see Fig 3 Cleanliness levels are designated as Pass A if the measured amount of luminescence falls below level 1 and as Fail C if the amount is higher than level 2 With Fail C cleaning will need to be redone and cleaning procedures may need to be revised Cleanli ness levels where the measured amount of luminescence is above level 1 but below level 2 are designated as Caution B and it is recommended that cleaning be redone and cl
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16. eaning procedures revised in such cases Examples of benchmark setting values for food production centers are listed in Table 3 The benchmark setting values for cleanliness level control shown in Table 3 are provided merely for reference and may not necessarily apply to all situations and cases The benchmark levels are important guidelines for determining cleanliness levels and should be set as appropriate according to required cleanliness levels Levell Level2 Para aa a at at at af ae Low Relative Light Unit RLU High Clean Cleanliness level Contaminated Fig 3 Setting Benchmark Level for Cleanliness Level Control Table 3 Example of Setting Benchmark Level for Cleanliness Level Control in Food Production Centers Relative Light Unit RLU Substance under Test Level 1 Level 2 Stainless steel surface 200 400 Resin surface 500 1 000 Hands and fingers 1 500 3 000 2011 Kikkoman Corp 20150301 Disposal Methods This kit contains no hazardous materials This kit can be disposed of as regular garbage but when disposing of it 1t would be better to separate the parts and dispose of each one properly in accordance with the local regulations outlined by the local govern ments for proper disposal of waste materials The main materials and parts used in this kit are listed below No PVC materials are used in the making of the plastics in this kit Table 4 Main raw materials of the structural parts of this product
17. ecking to see if the releasing reagent is leaking Q Set the benchmark level at which the required cleanliness levels can be obtained If the benchmark level is not set correctly cleanliness levels may not be evaluated correctly Secure the test tube of the LuciPac Pen to ensure that it does not become shifted or displaced It may be difficult to accurately determine cleanliness levels if the test reagent begins leaking because the tube has been shifted It may become difficult to remove the LuciPac Pen from the measurement device once the measurements have been taken Moreover it may cause malfunction of measurement device Designated Product for Measurement Lumitester PD 20 and Lumitester PD 30 Manufacturer Kikkoman Biochemifa Company Be sure to use only designated products when taking measurements LuciPac Pen Measurement Methods 1 Measurement procedures Complete the procedures listed below within appropriate temperature range as shown in Table 2 depending on an instrument used and temperature compensation setting Make sure to always run measurement tests at the same temperature to maintain repeat ability from comparison if the temperature compensation is not used Remove the LuciPac Pen from refrigerator and wait until they have reached room temperature see Table 2 Use the swab devices as soon as possible once they have returned to room temperature Remove the swab stick from the main body casing If running
18. id Pyrophosphoric acid Pyruvate orthophosphate dikinase Releasing reagent Surfactant Benzalkonium chloride Swab stick Orange Main body Fig 2 Each part name of the LuciPac Pen Precautions for Use Please make sure to follow the items outlined below in order to obtain optimal performance from this kit Do not use products that are past the expiry date Expired products may not yield accurate results the expiry date is printed on the bottom of the aluminum bag holding the swab devices 2011 Kikkoman Corp 20150301 Be sure to use only designated products when taking measure ments for amounts of luminescence This kit cannot be used with nondesignated products 3 The swab devices should be allowed to reach room tempera ture see Table 2 prior to testing if they are from refriger ated stock Measurement values may read lower than actual if the swab devices are used while still cold Use the swab devices as soon as possible once they have returned to room temperature Do not let the kit sit out in temperatures exceed ing 35 C 95 F High temperatures may cause product perfor mance to drop Table 2 Appropriate measurement temperature Temperature Temperature Model compensation range setting Lumitester PD 20 20 35 C 68 95 F 10 40 C 50 104 F 1 Temperature compensation is performed in accordance with the measured value of athermometer incorporated in the main bod
19. imitation The upper and lower temperature limits will be indicated on either side of the symbol Please store this product at the indicated temperature range Symbol for Caution or Attention for use Symbol for Lot Number This symbol shall be adjacent to the manufacturer s lot number e g 20150410T or description of its printed location Symbol for Use By This symbol shall be adjacent to the expiration date expressed as YY YYMMDD e g 20160709 or description of its printed loca tion Symbol for Consult Instructions Manual Symbol for Manufacturer This symbol shall be adjacent to the name and address of the manufac turer LuciPac and Lumitester are registered trademarks of Kikkoman Corporation in Japan and other countries Kikkoman Biochemifa Company 2 1 1 Nishi shinbashi Minato ku Tokyo 105 0003 Japan Phone 81 3 5521 5490 Fax 81 3 5521 5498 E mail biochemifa mail kikkoman co jp URL http biochemifa kikkoman co jp e 2011 Kikkoman Corp 20150301 LuciPac Pen Direction for LuciPac Pen lt Leave LuciPac Pen at room temperature see Table 2 until they have reached room temperature gt A Swab stick B Main body AF Pull the cotton swab stick A out Whenthetestobjectisdry of the main body B moisten the swab A with tap om Coa r water 4 or wet the object with gt C Test tube ater44 ON Swab the test object with the P
20. ith bare hands or allow it to splash into eyes 2 Please make sure to read the precautions and instructions in this Instruction Manual before attempting to use the kit and exercise extreme caution when using it The Kikkoman ATP AMP hygiene monitoring test kit LuciPac Pen is a kit for testing cleanliness levels using biolumi nescence techniques using firefly luciferase developed with Kikkoman s unique biotechnology Applications This kit can be used to test cleanliness levels using a swab test Do not use this device for any purpose other than what it was designed for as a tester of cleanliness levels Please be aware that this kit cannot be used to test or measure for counts of viable bacteria or more specific types of pathogenic bacteria This kit is used to indicate cleanliness levels in food produc tion centers and medical treatment facilities This kit shall not provide any guarantee that a given test environment is free of bacterial contamination This kit cannot be used to test food or food products directly Measurement Principles This kit uses an enzyme cycling method based on a combina tion of luminescent reactions from firefly luciferase and pyruvate orthophosphate dikinase PPDK This method produces a given amount of luminescent that is proportional to the amounts of adenosine triphosphate ATP and adenosine monophosphate AMP present Pyruvic acid Phosphoric acid Phosphoenolpyruvic acid PPDK AMP Pyrophosph
21. oric acid Luciferase Luciferin 02 Oxyluciferin CO2 light Fig 1 Principle of luminescence method using the enzyme cycling Firefly luciferase emits light in the presence of ATP and luciferin The AMP produced from this reaction is converted back into ATP using PPDK to enable a high but stable amount of luminescence to be obtained ATP is a source of energy necessary for various forms of life that is present in organic residues such as microorganisms food residue and biological substances that originate from other living organisms This method allows you to measure and detect organic residues at high speed and high sensitivity by detecting ATP using luciferase which is why it is widely used in determining cleanliness levels in food production centers and medical treatment facilities In addition this kit can be used to measure amounts of not only ATP but also of AMP which is produced from the break down of ATP enabling it to be used to test for an even wider range of organic residues This kit is a simple integrated testing instrument that contains both the test reagent and the swab device required for testing cleanliness levels Contents The LuciPac Pen kit comes with five aluminum bags each containing 20 swab devices for a total of 100 swab devices Table 1 Main components of each reagent Reagent name Main component Luminescent reagent Luciferin Luciferase Magnesium acetate Phosphoenolpyruvic ac
22. ut the swab stick A back to the Shake the whole body ofthe swab stick A main body B and push it through LuciPac Pen a few times so that all the way by putting the tip of the the liquid in the capsule falls into test tube C on a palm of hand or the test tube C table Be careful not to get fingers caught when pushing it a Gently shake the whole body of Insert the whole body of the Press the ENTER key Results the LuciPac Pen so that the LuciPac Pen into the measure are obtained in 10 seconds luminescent reagent is entirely ment chamber of Lumitester dissolved And close the chamber cover 4 If the luminescence level of tap water shows high we recommend you to let the water flow a few minutes and or clean up the outlet of tap 2011 Kikkoman Corp 20150301 8
23. y of Lumitester PD 30 Consequently the compensation cannot be performed precisely if the temperature of the main body of PD 30 is different from LuciPac Pen Please be sure that temperature of the main body of PD 30 and LuciPac Pen become same before use Lumitester PD 30 Wherever possible be sure to use up all swab devices from a single bag that has been opened at one time If you absolutely must store leftover swab devices once finished with a test session firmly close the aluminum bag and store it in a refrig erated environment 2 C to 8 C 35 6 F to 46 4 F High temperatures may cause product performance to drop Do not subject the kit or any part of it to direct sunlight for long periods of time Strong light may cause product perfor mance to drop Do not touch any of the parts inside the sampling devices particularly not any part of the cotton swab itself with a finger or other object before use Touching the parts may affect cleanliness levels making them hard to determine Do not drop the kit or any of its parts or allow any parts to be struck or jolted The inner aluminum sheets and other parts in the kit may become damaged causing product performance to drop Do not use the kit if any parts become damaged such as the inner aluminum sheet Such damage may affect product performance causing cleanliness levels to fail to be measured correctly You can tell if the aluminum sheet is damaged or not by ch
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