動物種判別LAMP法用プライマーセット ブタ用 取扱説明書
Contents
1. 1 LAMP DNA LAMP2. LAMP LAMP
3. 0 5 0 5 1 gt i i
4. 3 2 Tt DNA o Distilled Water e PC Ssd lt DNA 100 ng DNA 10 ng DNA 1 ng DNA 100 pg 30 DNA 10 pg 1 LT 16 DNA Distilled Water e PC Ssd DNA 100 ng DNA 100 ng DNA 100 ng 9 DNA A DNA
5. A 0 5 B A Positive Control Ssd Positive Control Ssd
6. ISO 13485 2003 http www nippongenematerial com II LAMP 48 NE3021 Primer Mix Ssd PM Ssd 125 pL 20C DNA 12 5mLx4 20C Positive Control Ssd PC Ssd 40 pL 20C 20C 6 DNA
7. 2 3 1 5 mL 2 0 mL 1 x 3 25 uL 1 8 1 10x A 2 5 uL 22 5 pL Primer Mix Ssd 2 5 pL 22 5 uL dNTPs Mixture 1 4 pL 12 6 pL 1 0 pL 9 0 pL 1 0 pL 9 0 pL ddWater 15 6 nuL 14 6 pL 140 4 nuL 131 4 pL 23 0 pL 207 pL 2 4 Loopamp 23 pL Loopamp 2 5
8. e Genome Order http genome e mpjp LAMP LT 16 NE4011 http nippongene analysis com TEL 076 451 6548 FAX 076 451 6547 p I LAMP LAMP DNA A LAMP DNA LAMP DNA
9. 2015 5 LAMP NE3021 LAMP 1 LAMP 2 20C 6 3 4 LAMP DNA
10. Loopamp NIPPON GENE TEL 076 451 6548 TEL 03 6712 3885 FAX 076 451 6547 FAX 03 6712 3886 E mail support nippongene analysis com E mail feast egenome csjp fujitsu com URL http nippongene analysis com URL http genome e mpJp Copyright 2015 NIPPON GENE CO LTD All Rights Reserved
11. A NE6021 Loopamp DNA 5 6 LAMP 7 LAMP 8 LAMP e Genome Order
12. DNA DNA 2 LT 16 3 3 3 4 DNA NN 2 V
13. 1 5 mL 2 0 mL LAMP LT 16 NE4011 1 5 mL Loopamp IV Positive Control Ssd Positive Control Ssd 2 0 pL 25 0 uL 1 LAMP 64 C 30 LAMP 3
14. DNA DNA 100 mg 1 5 mL DNA 1 mL 56C 15 1 x 3 2 2 1 Primer Mix Ssd Positive Control Ssd LAMP DNA A 10x A dNTPs Mixture ddWater 20 C 2 2 1 x3
15. gdWater 2 0 pL 1 DNA 2 0 LL Positive Control Ssd 2 0 pL 2 6 Loopamp 64 30 2 5 2 7 80 5 3 3 1
16. B A B DNA DNA 50 DNA VI 1 Notomi T Okayama H Masubuchi H Yonekawa T Watanabe K Amino N Hase T 2000 Loop mediated isothermal amplification of DNA c e c Acids Res 28 12 e63 2 Prince AM Andrus L 1992 PCR how to kill unwanted DNA Biotechnigues 12 3 358 2015 5
17. LAMP DNA DNA DNA LAMP DNA DNA DNA LT 16 LAMP LAMP DNA A DNA
18. 5000 ppm 0 5 iij 5 iv 5 1 DNA DNA DNA DNA DNA DNA DNA 1 mM EDTA DNA 10 mM Tri HCI pH 8 0
19. 2 PCR PCR DNA LAMP Loop mediated Isothermal Amplification LAMP DNA DNA LAMP Eiken GENOME SITE http loopamp eiken co jp amp amp
20. 20 DNA 20C Positive Control Ssd DNA 1 I LAMP DNA A NE6021 Loopamp 0 5 10 uuL 10 100 unuL 100 1 000 uL
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