ReproCryo DMSO Free™ プロトコル
Contents
1. n 3 Q1 80 A1 3 6 Q2 A2 0Q3 ReproCryo DMSO Free A3 ReproCELL Inc https www reprocell com E mail info_jp reprocell com FOR RESEARCH USE ONLY2. 1 80 6 3 6 1 ReproCryo DMSO Free 20 2 ES iPS 3 PBS 2 mL 4 PBS ESGRO complete ACCUTASE 1 mL 5 37C 596CO gt 10 6 1 mL 7 P 1000 15 mL 8 300xg 5 9
3. www reprocell com ReproCryo DMSO Free ES iPS 15 mL 10 ReproCryo DMSO Free 30 1 80 BICELL 3 6 10 ReproCryo DMSO Free gt ES iPS gt ES
4. 4 mL 10 pM Y 27632 24 Y 27632 37 596CO gt www reprocell com 80 70 60 50 40 30 20 10 0 1 2 3 4 1 Lot ReproCryo DMSO Free iPS 60 909 Lot n 3 1300000 1200000 1100000 1000000 900000 ReproCryo DMSO 800000 700000 600000 500000 400000 0 3 2 3 ReproCryo DMSO Free
5. 0 5 mL 10 0 5 mL ReproCryo DMSO Free 10 15 mL 11 37 30 12 10 P 1000 2 13 30 15 mL 14 4 80 3 6 1 80 15 FOR RESEARCH USE ONLY REPROCELL 16
6. ES iPS 10 12 13 7 60 mm 8 10 37 10 mL 5 2 P 1000 2 15 mL 1 mL 10 15 mL 2 5 mL 30 15 mL 5 300xg 5
7. REPROCELL ReproCryo DMSO Free Cat No RCHEFCO002 Version 1 0 ES iPS 20 2 8 20 2 iPS Takahashi K et al Cell 131 861 72 2007 pH DMSO Chemically defined Xeno Free
8. iPS gt FOR RESEARCH USE ONLY REPROCELL ReproCryo DMSO Free BICELL Mr Fr osty Millipore SF006 100 mL 4 C Complete Accutase PBS Ca and Mg Free 60 mm Tissue Culture Dish www reprocell com ReproCryo DMSO Free 1 4 2 37 ES iPS 1 60 mm 2 ES iPS 3 ReproCryo DMSO Free 15 mL 10 4 ReproCryo DMSO Free 30 5
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