取扱説明書
Contents
1. 1 5 Genotype E coli DH5oe geoR endAl1 gyrA96 hsdR17 rc mk phoA recAl1 relA1 supE44 thi 1 A lacZYA argF U169 80dlacZAM15 F A 6 gt SOC 1 3 SOC 13 1 102. 1 SOC SOC Competent high DH5w Code No DNA 903 Competent Quick DH5 w lt DNA 5 42 C 30 LB 37 C 1 37 DNA
3. 2 DNA 30 2 DNA 100 Il 10ng 3 3 LB 50 100 g ml 10 50 ug ml X gal 40mg ml X gal 20 LB 4 50 1 0E 09 1 0E 08 Amp Kan 1 0E 07 Tet Cm transformants g plasmid 1 0E 06
4. 1 5 2 10 2 Competent Quick DH5w 100 x 20 3 1pg pBR322 LB 1 x 10 transformants g pBR322 gw pUC19 LB X gal 99 4 80 C 80 3 3
5. DNA 100 10ng 7 Hanahan D J Mol Biol 166 557 1983 Inoue H Nojima H and Okayama H Gene 96 23 28 1990 8 Code No Competent high JM109 lt EE coliJM109 SOC gt Competent high DH5 lt E coli DH5 SOC gt Competent high DH5 gw lt E co7 DH5 gw SOC gt SOC 1ml x 10 pBR322 100 x10 DNA 900 100 x10 DNA 901 100 x10 DNA 903 HPCR 530 8230 2 8 TEL 06 6348 3786 FAX 06 6348 3833 E mail order_lifescience toyoDbO jp 104 8345 17 10 TEL 03 6887 8819 FAX 03 6887 8951 E mail order_lifescience t
6. SOC SOC 30min 60min 1 pBR322 Ampicilin Tetracycline pAK119M Kanamycin pAT5 Chloramphenico 10pg 5 TE buffer SOC 37 30 60 10 10 transformants gplasmid SOC 10 LB
7. Amp 50 z g ml Kan 10g ml Tet 20 g ml Cm 100 gml 10E 09 FE 1 0E 08 es ersdiozoc 1 0E 07 E transformants g pBR322 1 0E 06 0 10 20 30 40 50 60 100 plasmid D 2 DNA pBR322 Ampicilin 5pg 5 50 Il TE buffer DNA 309 1 0 E 09 1 0 E 08 a 1 0 E 07 transformants g pBR322 1 0 E 06 1 10 100 1 10 100 pg pg pg ng ng ng 100 plasmid 3 DNA pBR322 Ampicilin 1pg 100ng 5 TE buffer DNA
8. 14 2 Pi ldeas amp Chemistry E coli Competent Cell Competent Quick DH5 o Code No DNA 913 A I C E 1 1 AS3981K 1
9. oyoDbO jp TEL 06 6348 3888 FAX 06 6348 3833 9 00 12 00 13 00 17 00 E mail tech_osaka toyobo jp URL http www toyobo co jp bio
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