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G-Track取扱説明書ファイル

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1. Glrack 37 www 4 4 Trace Analysis 0000000000 Trace Analysis trace number is DA Data 52 al Trace SYNC Frame Replay 404 9 4045 404 7 2215 222 2225 Position pixels 405 405 P 404 2 50 a Triming Copy Clear SAVE Histogram F otationi Difusion Constant MONE 441 Trace Analysis Total trace number s b 52 lt Trace SYNC e Frame Centroid Replay Trace Analysis FA 404 8 000000000 4047 ET RIF 4046 GTrack 38 G AngstirOm HE Data Frane No Positionlpixels Y Position pixels X 5 SD pixels Intensitty 0000000000 Data Frane No Position pxels X 5
2. 462 1 O Glrack 10000 44 Co G Angstrom Histogam 00 0 I 00 0 0 0000 0 Glrack 45 4 7 Histogram 00000 0 Analysis Histogram placement m 1 r z x Time Value 0 07 4 Velocity Histogram 2 0 0 5 1 Velocity 0 0 83 Bin 20 Fitting 000000000000000 Velocity Intensity X 500 Y 500 Duration Distance 0 O 000000000000000 Histogam 0000 L Histogpam 10000000 00000000000000000000 Velocity Intensity X SDO 01 Duration Distance 0000000 46 Co G Angstrom save Histogam HH 00000 00 00000 UU Velocity Intensity X SD Y 500 Duration Distance 00000000 Histogam
3. 5 5 P 2 gt A z e e 5 je L 5 FR gt 5 E s 5 la HHE 8 s x 8 88 5 81 575 8 al HMM G Angstrom 15 G Track G Angstrom 3 2 1 00000000000000000000 Open PJ Save PJ Speedy Clear Dat Save Dat ffo Trace SYNC W Frame Centroid Replay Video Control Scale 1 pixel 1 00000 lt Slow PlaySpeed High 100000 s Gain 5 25 Threshold ROI Size 5 Pixeis 2 Fhimit inverse ColorBar Information Raw Data ROI Gaussfitin ROI Time s Min 1000000000000000 00000000000 1 Frame s lt X Position Y Position 10 8009 8060 Save Frame s 5 0 510 Method D htensity Centroid BG Fixed Initialize Gauss Fitting ROI optimization 2DGauss Fitting Histogram Rotation Diffusion Constant NONE Triming Copy Clea
4. 27 G Track Co G Angstrom 413 00000000 CD v BRERA Ei Trace Analysis 414 000000000 MH E ts D Dis ARRA G 000000000 000000000 000000 Glrack 28 G Angstrom 42 G Trakk 1110000 G Track untitled BRAD AJAH 25 Om 82 New PJ Save Import speedy Clear Dat Save Dat hfo Control 4 Slow High Threshold Size k Pixel s Fl lirnit Information aw Data in ROI GaussFitin ROI m Integral Frame s 21 Room SE 5000 Save 24 21 HA Method Centroid 2 BG Fixed Gauss Fitting optimization 2DGauss Fitting Raw 94 9g Ne
5. 00000000 Histogam 10 0000000000000 l 8 Histogam 0000000000000 Auto Histogam 0 00 Glrack 47 Co G Angstrom 4 8 Rotation UU UU DU U UU L Analysis Rotation 180 sa sr 0 5 9 EP E 7 immi Y Position A 130 Step 45 0 0 00 Drigin Fitting 180 481 RotationI 0000 Rotation JU 0000 0000 DU L Rotation Glrack 48 Co G Angstrom 482 Rotation O000000000000000 4 8 2 2 X Y
6. o o aA O O A a CD ROM e 100000 95800000000 G Track Co G Angstrom e e o o Penti umlll 000 308000 0000000 7200 000 00000 8bi t xA 249 20 Nati onal nstrunents 8 2 1 Saf eNet Senti nel 0 0 0 X0 G 0 00000000 Glrack 10000 6 Co G Angstrom 13 UUUDDUDUDUUDUBUDUUDUDUUDDUDDUUDDUUDUDUUUUU 50000000000000000000000000 00000000000 http ww g angstromcom 00000 Copyright 2006 2008 G Angstrom All Rights Reserved Microsoft 8 Wndows 10 Microsoft Corporation 000 0
7. O Bffusiononstart 0000000000 m 3D 390 00000000000 I nfornati on FI in 30 RO 0 3DI 0 0 U Y xY DOL Y 00000000000 05 E GO in GTrack 10000 21 G Angstrom A RTA FAIRIES 0515 1 202 K Ctr F041 EBA SG HIGH ag x Speedy Clear Dat Save Dat hfo PlaySpeed High Scale pi pixel 1 00000 nm 1 fram 100000 s Thrachalad in FO 1000001 H 00000 H stogrami 0 acenent Position 1 0 spl acenent Positi 1 Diffusion Constant MSD ffusi on Constant H stogramni 110000 1000000000 gt 10000000 gt 000000000 G Track 22 Co G Angstrom JU Glrack 10000
8. L Posting 0000000000 00000 Posting 000000000 Glrack 49 Co G Angstrom 49 Rotation Analysis Rotation Y Position 1 s 1 1 Position ima 17 step 45 00 2 Dizplace ment E 18 2 Time j 180 rizin 60 120 180 Save XY 1 0 00O 00000000000000000 wy 00 00000 X Y Position 000000 sel LIL C sted 00000000 Position Glrack 50 Co G Angstrom HUU L 0000 55 stepi 00000 X Y 0 0000000000000000 00000 X Position Y
9. Fran X Posi ti on pi s lt 1 H 30 Y Positi on pi xel s X SD pixels Y 50 pi 5 ntensi ty A U F 2 DataNo 47 4 jr a O 1 2 3 4 5 6 mes 7 Fa HRTF A ET MU Trace Anal 0000 HUUDUDUUDUUDUDUHUDUDUUDUUUUU 00000000000 Glrack 18 G Angstrom SM R RTV v 5 50 Xan 2 44 MS h 0 B m 2 7 GTrack 10000 560 0 000000000 0 1000000000 MU Trace Anal ysi s OD O D 0 000000 000000000 00000 0000 L stogrand 0 acenent ffusi on Constant ff usi on Constant 30000 stogrand Trace Analysis 100 0 stogr am 00000000000 Hstograml 000 000000 0
10. 2 3 USB usg 1 J 02 02 52 05 C 9581000000000 PC etupexe Sentinel System Driver Installer o m o0 x x ong Glrack 11 Co G Angstrom 3 Glrack 10000 G Angstrom 31 O00000 L 1 0 00000000000000 L0 0 Track 1 20 000020000 00000 L GTrack 10000 13 o a00 9 a d o a00 7 COOS 5 2 0 o 2000p 9 BILI a N a 52
11. o OO Regon Of Inte ROID 00000 0000 00 00006 4 3 2 43 4 3 1 Co G Angstrom FI in RO n GF i 36 G Track Co G Angstrom in ROII I L GF 0 0001000000000000000000000 Gaussian Fitting L 20 Gaussian Fittind 00000 00000000000 0000 00000 433 0 0000
12. 0 000 000000000000000000 1000 00000 00 00000 000 1 29 0000000000000000000 455000000 o0 X0 221000 Glrack 10000 54 Co G Angstrom 0050200 000 2D Diffusion Constant 60 5 A 2200000 4000000000 6 savei Diffusion Constant Histogam 000000000000 Diffusion constant X nrr 2 s Diffusion constant Y nm2 s Diffusion constant 2D nm 5 0000000 Diffusion Constant Histogram O 0 0 Diffusion Constant 00000000 Diffusion Constant Histogram 00000 00000 0 D Diffusion Constant Histogram 0 00000 0000 00 Diffusion Constant Histogram 0 00000 0000 00 Autol Diffusion Constant Histogram
13. 000000 Centroidl 000000 Y 00000000000 Intensity 0 0 0 0000000000000000000 O 000000000 00000000 000 a a a GTrack 10000 42 Co G Angstrom Clea 000 00000000000000000 Savel Position pixels Y Positionlpixels X SD pixels SD pixels Intensity A U Glrack 10000 43 Co G Angstrom 4 6 Histogram UU U UU D U l nalysis Histogram 4 Displacement tima gt _ E 2305 R E coc ___ 2 Time 2 Value 10 07 Y velocity Histogram Frequency a Velocity ma Anto 461
14. gt x G Track 0O O Microsoft Framework Version 2 0 0 0 0000000000000 Udotnethxexe Framework Version 2 0 gt 0000000000 00000000000000 EW8 2 1 Runti Engine LabM 0000000000000000000000 LD 2907 1 Sand System Ormar 4 74 laxa 10 G Angstrom 6 0000000000000 Senti nel Installer 7 4 0 acd gt V 25 s 2 1 Lab VIEW 8 21 Funtime Sentina Systeam Driver h tallar TAD 98 000000000 000000000 200
15. Ss Ga OAC oo to oo a 5 GG OOC F e lt oo ooon ga ga g lt o d Do OoOo o0 L L O EA o o oo oo go oo Sono o O goo oooga x E 9 G Angstrom 14 UUU initialize 000 G Track 5001000 00 0 0 0 0 0 0 0 0 0 1000 0000 l 0 2 Ctrig 0 O 0 0 0 0 0 0000 1 O Run 000000 UUU 5 00000000000 Trace Anal ysi s 4 s 5 47 5 gt 8 8 2 48 w 8 gle x 7 4 ss 5 a 2 gt z 3 ZEE 8 55 5 e E 8 p
16. Co G Angstrom Slow 000000 0000000 0000 000 COLORBARO Glrack 31 Co G Angstrom INFORMATION Information aw Data GaussFitin ROI 1 9 sae FI in 3D0 0 FOII MU Fluorescen
17. RA 522 0 00000060 m unnnn 000000000 000000000 000000000 00000 523 0 GTrack 10000 57 Co G Angstrom Fl in ROIO 320 0 0 T GF in ROID DU D 00 35000000 00000000 0000000000000 ROND O 524 248900 2 LIC 1 1 1 532 010 00 PCOO000000000000 00000000000000000 000000000000000000000000000 00000000000 0 0 6 ver121 000000 000 O O O 2008 G Angstrom HHA OEE E E E E Glrack 10000 58
18. Triming 09 1000000000000 5 000000 0650000000000 19 PJ Open PJ Save PJ D Speedy Clear Dat Save Dat Info Video Control Scale 1 pixel 1 00000 nm 1frame Slow PlaySpeed High San 100 7 5 3 7 Offset R 3 3 2 8 inverse 1 lt o 10 Color ar 1 00000 s ROI Size 5 Pixel s Linformation Raw Data in ROI GaussFitin ROI 5 Frame 5 i 25 Integral 1 n x Y 8009 sp Room Frame Method Centroid Gauss Fitting ROI optimization 2DGauss Fitting Threshold Initialize Trace Analysis Total trace number is 52 paano a7 215 Trace SYNC Frame Centrold Replay 9 10 W Time frames Intensity Triming Copy Clear Save Run Histogram Rotation G Track untitled DE NUM 5 D New PJ Open PJ Save Speedy Clear Dat Save Info 1 Video Control J Scale 1 p el 1 00000 nm 1 frame
19. G Track JU 1 0000 JL JJ PT au 5 Jo 6 RN 1 2 100000 L ran ns 22 A ia 9 A A IR 11 0000000 sL 13 16 uuu _____________ ____ 18 4 100100 I usss Y UR RE I 24 42 G Track 0100000 29 Aa 36 44 TraceAnalysis 00000000000 a 38 45 41 46 Histogam 44 47 DUO UT OEN i inn A gaga 46 48 0000000000 00 48 49 Q i erop 50 4 10 Diffusion Constant 0 0000000 00 52 411 Diffusion Constant 00000000 ar 54 5 5 1 G Track A SB tanga 56 2 Co G Angstrom se ta 57 53 l G Track ver1 21 T si an 58 GTrack 10000 3 Co G Angstrom Glrack 1000 Co G Angstrom 1000 11 e e
20. Pentium Intel Corporation L National Instruments LabVIEW O Nationalinstruments Corporation OO 00000 0 0 0 0 0 00 0 0 0 0 0 0 0 0 Sentinel 000 00000 Glrack 7 Co G Angstrom 2 Glrack Co G Angstrom 2 21 O00000 L 120000000000000000000000000 22 0000000 o 1 2 000000000 00000000000 50000000 LU Glrack 9 G Angstrom 3 ver 2110000000060 VM ANIH Per Ee E 7425 0 setup L 21 0000000000 9458000000000
21. Dat Save Dat Trace SYNC Centroid Video Control Stale Frame Centrol BA 84 2 5 offset a Inverse Color ar 10 1 1 Y li Room 8000 0 Frame 5 Trace Analysis O 00 on ULDiffusion Constant 000000 8 T 1 40 2 4 6 8 Time 6 Diffusion Constant nm 2 s Origin 180 Min Max Bin Auto 20 10 Glrack G Angstrom ws 3 3 1 00000000000000000000 DataNo 47 D Open PJ Save D peedy lear Dat Sa at Trace SYNC 7 Frame Centrold Replay Video Control hr 1 1 00000 5 26 100000 s 24 100 to lll Alla ls 10 2 3 4 50 6 70 Time frames 1 4 22 182 22 move avi 47 csv 204 356 175 356 180 5 1 Frame s X 2 oor NORA 0 50 100 0 50 100 Position Position 33 3 25 1 10 2 154 1 1 ar 0 50 100 0 5 0 50 100 XSD YSsD Triming Copy Histogram Diffusion Constant 21244642 2
22. Integral Frame 5 X Position 2 1 1 Velocity nm s a ratan 50 10 0 50 10 0 50100 XSD YSD htensity in Max Bin Auto 8 Triming Gauss Fitting ROI optimization 2DGauss Fiting RawFl Run Histogram Rotation Diffusion Constant NONE GTrack 10000 000000000000 New PJ Open Save PJ Speedy Clear Dat Save Dat Info Trace SYNC Frame 7 Centroid Replay Video Control Scale 1 1 1 00000 nm Slow PlaySpeed High 1 00000 5 Displacement am Threshold ROI Size 3 5 jr 41 Inverse f au ColorBar L Information Raw Data in ROI GaussFitin ROI Time s 0 4 10000010 al Integral _ H mu 4 6 Velocity nm s 1 xs Intensity Max Bin hita Centroid Fixed Initialize Triming Clear Gauss Fitting ROI optimization 2DGauss Fitting RawFI Run A Histogram Rotation Diffusion Constant NONE 100 00 na Trace Analysis Total trace number is 52 Analysis Histogram H t Ez
23. O O O O Displacenrer Position Y Position HUUDUUDUBDUUU O ign X Y Position X Y Position 000 F 1 Position 0 0 X Y Position nmi GTrack 10000 51 Co G Angstrom 4 101 Diffusion Constant Analysis Diffusion Constant Ble Green2 0 ref MIDT A 65 AHi URTA EMPER 11 Time C x 0 221 0 036 2 0 257 11 g Mean square Dizplaceme a Frequency 2 4 Diffusion Constant 2221 in Ato 410 1 Diffusion Diffusion Constant Diffusion Constant Glrack 52 Co G Angstrom 4 10 2 Diffusion Constant
24. ffusi O rnst ant Trace Analysis 1 Constant 0 00000060 Corstart O00000 O ff usi on ff usi on x SD htensity 50 Triming Copy Clear Save Histogram Rotation Diffusion Constant NONE 10000000000 100000000 0 00000000000 Kala 10 m Y 2D 8 Dffusi COnstant 0000 00000000 I C G Track 20 0 G Angstrom Dittusion Constant Red Bhg 88 sell a 2150 8 gt MSDF a 197705 9 0 5 100 150 Time is 21 Inverse 1 mg DO ffusi O O onConstat 1 0000000000 5000 10100000000 00000 000000000000000 O Bffusiononstart 0000000000
25. 145 Er FRL 5 AT AF 5 EA EER 0 3 Glrack 25 Co G Angstrom 4 1 21 1 4 1 2 2 FERES ATAT PR Glrack 26 Co G Angstrom 4 1 2 3 P AS JUD U UI Iy 4 1 2 4 FENA A FEL
26. 00 0 4 10 21 0 500000000000000000 02 5 0 0 00000000000000 700 0250 MSD X MSD nrr2 Y MSD NMDA 2D MSD nm2 0 0 0 020 MSDN 0 X MSD NN DRD 5 020 X MSD nrr Y 1 20 MSD nm2 1 bpi 0 L 4 10 2 2 Diffusion Constant Histogpram 00000000000 Diffusion Constant Histogram 0000000 0000 00000 Glrack 10000 53 Co G Angstrom 4 110 Diffusion Constant 00000000 21 Diffusion Constant Red Green2 0 14 12 23 s 10 2 9 z 4 Diffusion Constant im 2 2 D Min 6 3 Bin 20 MSD 1 0 0 0 MSD Mean Square Displacerrent 220 5000000000000 24900000000
27. Hstograri 0 10 000000 Open Save PJ Speedy Clear Dat Save Dat Trace SYNC Frame Centroid Replay Video Control Scale J 1 pix 1 00000 nm lacement lt mi i 1 frame Slow PlaySpeed High 1 00000 Gain 5 4 6 Threshold ET 4 ROI Size 5 Offset 211 Ftllmit Inverse 10 AU Color ar 7 Information Raw Data in ROI GaussFitin ROI Time s 20 30 40 Time frames Min 182 5 Frame s Integral La 0 50 100 1 Frame 5 X Position Room 5 10 0000 1 1 0 Frame s 0 05 Velocity m s O LIL Method D in 0 04 0 83 Bin 20 Auto Centroid BG Fixed Gauss Fitting ROI optimization 2DGauss Fitting RawFI Histogram Rotation Diffusion Constant NONE 100 00 na Displacement 7 Y SD 1 100000 nm Slow PlaySpeed High 100000 s Loop Information Raw Data in ROI Gaussfitin ROI Time s ia 5 Rotation J J J Bffusion 1 a D Open PJ Save PJ Speedy
28. ROI 00000000 RAIN 00000 Regon Of Interest Fi limti IJ 000000 1 O O FI Fluorescence Intensity 0000000 40000000000000040000000 Inega 0 000000 Romi Initialize Glrack 35 Regon Of Interest 000000 Regon Of Inter u T x u
29. Slow PlaySpeed High Gain 7 E s y 9 7 Offset R 3 3 2 8 L inverse 1 9 j o 10 I ColorBar Linformation Raw Data in ROI GaussFitin ROI 1 00000 s Threshold RO Size 5 J 5 Frames Integral 1 Frame s n I 800 Method Centroid Gauss Fitting ROI optimization 2 Initialize Room Frame Diffusion Constant NONE Trace Analysis Total trace number 52 47 Trace SYNC Frame Centrold Replay tensity Triming Copy Run Histogram Rotation NUM Diffusion Constant NONE Analysis Rotation gt Y Position nm 5 0 X Position nm st 45 0 44 34 Analysis Diffusion Constant Red X BlueY Green2D 2 4 6 8 10 Diffusion Constant nm 2 s Bin Rotati Trace Analysis 000 on Rotation 0000 00000 Triming Copy Histogram htensity 50 Clear Save Rotation Diffusion Constant Rotati ong 00000 Save li 0000 SUL
30. 00000 00000 0 GTrack 10000 55 G Angstrom gt 5 1 JUD DDD USBI O 51 000000 9 800000000000 1458000000000 1 2 0 30 3 GTrack ver 2110000000060 00000000000000 EW8 2 1 Runti Engine 00000000060 LabM 0000000000000000000000 56 AFO r 25 s v 16 1 2 21 Lab VIEW 8 21 Fum iime Sentina Systeam Driver h tallar 1 98 000000000 52 52 00000000000000 C HIGH MODE 0000000000000 HIGH MODE MODEN 0 0000000000000000 2221 0000000000 0000000000 000000000
31. 2 Fixed Gauss Fitting optimization 2DGauss Fitting Raw 1000000000000 0101010100 Centroid Gauss Fit Gauss Fitting 2D Gauss 00000 Gauss 120 Gauss Fittingl 00000000 BG Fixed O O O Gauss 2D Gauss HUU U GTrack 10000 33 Co G Angstrom otim ation 000 0 0890 90000000000000000 Fluorescence Intensity 00000 Ref 00000000 Back Ground 0 0 427 Thresold Threshold Size Integral 5 Elmi Room Frame s initialize ROI Sized 0 000000 0000000000 Size Glrack 10000 34 Co G Angstrom 0 0000
32. 5 Y SD pixels IntensitXA U J O0 1000000000000000000 Data Frane Positionlpixels Y P osition pixels X 5 Y SD pixels O0 1000000000000000000 442 Analysis Analysis Total trace number is 52 52 gt Trace SYNG Centroid Replay 2 2 LTR 2 Time frames Trace 5 1 0O 0000000 0 0 0 00 0 0 Co G Angstrom Trace Analysis 443 1 br 000000 444 Analysis 0 405 1 222 404 Position Y Position 81 htensty Clear Save Trace Analysis Glrack 40 G Angstrom 4 5 Trace Analysis 00000000 Trace Analysis Tota
33. Co G Angstrom 4 41 0000000000 Gtri N 2019 CP Ctrl 0 UUU JOSIF 00000 202170502 Gtrl G 0100000000 A TC A p ADP DAD Ctr AVII MD TF 1 3 00 000 Alt F N 00000 00 00 0 9100000000000 00000 80 0000000 Glrack 10000 24 Co G Angstrom 91100000000 00 000000 41 2 0000000000 1 HERRA D HERED
34. ce Intensity X ROII MU GF in 39000 RODO 00000000 00 0 Gaussian Fitting 00000000000 00000 X 52090 So 0000000000000 0 VU 0 000 90 50090 0 0000 0 969 in ROI 300 000000000 0000000000000000 50 500 U DU U GF 424 390 0 0 GF in ROD 3D0 000000000 GTrack 10000 32 Co G Angstrom 0 000000 425 Scale Scale 1 pixel x 5 1 1 0 1 00000000 426 Method Method
35. l trace number is 52 Data 52 Trace T SYNE Frame pixels 50 ifra 7405 2405 404 5 4045 404 21 50 Clear Save 13 Histogram F otationi 44 Difusion Constant Data 00 000 UUU 00000000 HU UUU Data o o 9 L O 1 41 Co G Angstrom Centroid 0000000 00000000000 00000000000 5 Stopl Stopil C 0 00 00000000000 xX Position 0 00000000000 Position 0 SD 0 Gaussian O OD 20 Gaussian 0000 0 00000 000 00000 000000 000000 X SD pixels 00000000000 SD 0 Gaussian Fitting O O O 2D Gaussian Fitting
36. r Save 100 00 na 00000 10000000000 000 New PJ Open Save PJ Speedy Clear Dat Save Dat Trace SYNC Frame 7 Centroid Replay Video Control Scale a 12 Threshold ROI Size 5 Offset 41 Inverse AU Color ar Loop Information Raw Data in ROI GaussFitin ROI Time s Y SD pixels Integral 1 Room Frame s 1 4 1 a 0 50 100 tensity Centroid BG Fixed Initialize Gauss Fitting ROI optimization ZDGaussFiting RawFI Histogram Rotation Diffusion Constant NONE 0000 g 28 a H L 0 100 5 a Hstogra New PJ Open PJ Save PJ Speedy Clear Dat Save Dat Trace SYNC Frame F Centroid Video Control Scale 1 1 00000 1 fram Slow PlaySpeed High 100000 8 Pani Mo Threshold ROI Size Pixel s Trace Analysis 0 0 H HU Displacement nm Y SD ixels Information velocity _ gt Raw Data in ROI Gaussfitin ROI 1 Time s 3 4 Histogram al Min 5 Frame s
37. w PJ Open PJ Save Import Speedy Save Dat Open PJ O O O L ERNI 0000 00 000 Save PJ J J 000010 GTrack 10000 29 G Angstrom Irrport HIGHMODE Speedy 1010 Clear 0 0 0 Sava Data Frame Positionlpixels Y Position pixels X SD pixels Y SD pixels Intensity A UJ II I I 00000 0 L 422 VIDEO ii Control 4 ow FlaySpeed High VIDEO CONTROLO 0 0 VIDEO CONTROLO 0 2000000001 Glrack 30

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