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Gene Pulser MXcell 取扱説明書

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Contents

1. Anderson 5 1991
2. 7 gt 10 cell mL
3. se1ect Protocol Set up in the home scree7 o e protoco1 Set up Gee Pulser MXcell ENTER
4. No Nn KR Q NH BE io ag EM67070 EMC EN61326 Class A
5. 7 In Vo V t RC droop droop Vo Vi Vo
6. 1 Bio Rad www bio rad com OB EG L Bio Rad Gene Expression Gateway www bio rad com genomics Search Bio Rad
7. 50 7 5000 Ge je Pulser MXcell 45 Gene Pulser MMXce A Pulse Trac Gene Pulser AXce Pulse 77ac Pulse Trac Pujse Trac
8. C C 3 C
9. PULSE PULSE pu se being delivered J Gee Pulser MXcell
10. Daja Management Data Management 1 Home screen Data Mamagement Data Mamagement ENTER 2 DataManagement
11. Ge e Expression Gateway www bio rad com genomics Gene Pulser MXcell Gee Pulser MXcell VAT Gene Pulser AXce7 2 2
12. Bio Rad FCC ClassA FCC Bio Rad FCC ClassA EMC EU EWC CSA Canada ClassA vii Gene Pulser MXcell A
13. 72 24 96 3 96 24 24 72 72 96 24 72 optimization Gene Pulser MXcell optimization Bio Rad Bio Rad
14. viii 5 6 CO n qe AE 1 Gene Pulser AXce 7 Bio Rad V 2 Li 3 Na 5 URAT LOR EES PT YT EEE 5 Gene Pulser MXce 7 6 P D E a EOE E A E E E AE E E E 8 Quadrant uuu 72 ei 14 14 ma n E AE A OEE L 16 ee er ee pum 17 A Ri 18 Protocol Se Ub Sn 18 Gradient Protocol 7 2 AA ieni e N 20 User Protocols 22 Pre Set Protocols AF 34 3 34st paie tte e Ete e teet eese tese DE gt 23 Last a EE AE 24 Data Manageen7 24 Screen eps 25 easure7eng 25 2 2 7 26
15. 70 1000 Gene Pulser MXcell Pujse 77ac Pulse Trac Pulse Trac P u se Trac
16. 7 CHER 44 77 V 7 e uF Vy e T SUR 7 T xC 6 t e 37 Vj Vo e Rr Fisampie Rec Rsampiet Rec PC
17. EUrsE 7 24 as Pulse Last Pulse rast Pulse Last Pulse 1 Home screen Last Pulse 2 ENTER Data Management 24 Data Management 700
18. Gene Expression Gateway www bio rad com genomics Life Science Research discover bio rad com Bio Rad PDF 4gope Acrobat Reader www adobe com
19. DNA Chu 5 798
20. cm 2 E Wd 9 Hui 1995 Neumann 2000
21. Gene Pulser MXcell 7 24 42 WL 5 Gene Pulser MXcell PULSE Exponential Decay Pulse Gene Pulser MXcell
22. EN61010 Bio Rad EN61010 vi FCC US FCC FCC Part15 ClassA
23. Gene Pulser MXcell Bio Rad Gene Pulser MXcell Gene Pulser MXcell Gene Pulser MXcell Gee Pulser MXcell Class 4
24. 6 Gene Pulser MXcell Gene Pulser MXcel 3 R 3 3 Gene Pulser MXcell Shift 2 a apc 7 ENTER abc 2 2
25. ENTER p EUrSEg PULSE PULSE 7 24 Last Pulse QUADRANT Quadrant 1 Home Gradient Protocol ENTER Plate 96 24
26. User Protocols Bio Rad 27 Pye Se Protocol pre set protocol 1 Home Pre Set Protocol ENTER 2 pre set protocol ENTER
27. 6 4 JL ABCD1 AB2 CD2 Quadrant 24 96 Quagra7 96 Quadrant lt 6 24 Quadrant 6 Quaoran7 9 96 24
28. 36 Uniform 96 well Exp Sgr Mixed 96 well Exp Sqr 76 95 COT 250V 350 uF 250V 20ms 16 Mixed 96 well Exp Sqr Exp Sqr Exp Sqr IExp Sqr Exp Sqr Exp Sqr Exp Sqr 1 2 3 4 5 6 7 8 9 10 11 12 A B C D E F G H Mixed 24 well Exp Sqr 24 37 0 I Mixed 96 well Exp Sgr Mixed 12 well Exp Sqr 7
29. in vivo siRNA s MA s MA siRNA siLentMer Dicer Substrate siRNA duplexes
30. PULSE 7 24 as Pulse User Protocols 22 User Protocol User Protocol User Protocols User Protocols 1 Home User Protocols ENTER User Directory User Protocols ser Protocols
31. B4 2 96 24 72 5 96 3 Gene Pulser MXcell Gene Pulser 7000 Gene Pulser AXce Gene Pulser AXce 7 Gene Pulser MXcell 8 9 Home
32. 78 Gradient Protocol Quagran gradient protocol 20 User Protocols 22 Ere Set Protocols 23 Last Pulse 24 Data Management 24 Screen Tntensity 25 Measurements 25 26 User Protocols 2Z feit
33. 19 Mixed 96 96 250 350p 250 20 ms 20 Mixed 24 24 MR 250 V 350p 250 V7 20 ms 21 Mixed 12 12 ME 250V 350uF 250 V7 20 ms Mini Optimization Protocols Mini Optimization Protocols 4 6 Opt mini 96 well Sqr Exp Z 99 ABCD1 lt 3 4ER 200V 2 000 uF 7 0000 20ms ABCD4 6 RAR 250V
34. Gradient Pro7oco7 24 96 gradient protocol gradient protocol GRADIENT gradient protocol 27 QUADRANT 7 gradient protocol 27 Well 96 24 1 2 3 4 5 6 7 8 9 10 11 12 Quadrant 1 Quadrant 2 Iiloj nm oloo zal fc a a ad ant 3 l Obadiant 4 10 96 24 Quadrant Quadrant FE 1
35. s MA4 DNA Gene Pujser PBS 5096 209 0 C
36. 6 29 6 Pre Set Protocol 4 6 31 1 Opt mini 96 well Sqr Exp 24 2 Opt mini 96 well Sqr 24 3 Opt mini 96 well Exp 24 4 Opt 96 well Sqr NP D 16 Whole plate protocols 33 5 96 well Exp 96 6 24well Exp 24
37. 18 Gene Pulser MXcell 107 500 V 2 25 2475 uF 25n 50 1 5009 502 00 102 10 500V 1252 Gene Pu ser 10 500V 0 05 9999 95 7 3 0 7 10 79 19 Gene Pulser MXcell 100 120 VAC 220 240 VAC 50 60Hz 240W 0 35C 0 9596 EN61010 EMC EN61326 Class A 31x30x14 cm BS 6 62 kg 50 20 20 Gene Pulser MXcell
38. 1 Home Protocol Set up ENTER ENTER WELL SET WELL SET ENTER Plate 96 24 12 Program WHOLE PLATE WELL SET ENTER Gene Pulser MXcell 5 exp soR ENTER p
39. 30pp dsRNA siRNA E siRNA V4 siRNA siRNA siRNA 10 700 nM siRNA siRNA
40. Gee Pulser AXce Gene Pulser MXcell 3 20ms 48 Gene Pulser AXce C Anderson M L M Spandidos D A and Coggins J R Electroporation of lymphoid cells factors affecting the efficiency of transfection J Biochem Biophys Meth 22 207 1991
41. CC 75 Gene Pulser MXcell 798 35C 9096 40 65C 90 132 VRMS 198 264 VRMS 47 63Hz 600 VA Gene Pulser MXcell Gene Pulser MXcell 7
42. DNA siRNA VM4 DNA SR Barsoum 1995 DNA Chu 1987 Showe 1990 39 Gene Pulser MXcell
43. ENrER User does not exist Create this new User Press SAVE if Yes Press BACK if No 4 S ENTER Save C1ear 5 Save Protocol has been saved under Mike 15 CHO 6 save BACK
44. Screen Intensity Measurements Gene Pulser AXce Quadrant Gene Pujser 3 96 24 72 5 7 5 96 1x 7 gt 2x 10 100 200uL 24 24 5x 10 8x 10 500 800uL 24 72 7x 10 1 5x 7 1 0 1 5mL 72 74
45. Vo v Vo 1i exponential decay pulse square wave pulse x B 77 groop Gene Pulser MXcell Gene Pulser Xcell PC Gee Pulser AXce resistor pulse modulation L T RPM Gene Pulser MXcell
46. 76 e Gene Pulser 165 2676 165 2677 FBS ED74 RA PBS 137 mM NaCl 2 7 mM KCl 9 5 mM pHZ7 3 Gene Pulser MXce Ge e Pulser AXce Gene Pulser MXcell 78 Protocol Set up
47. Potter 1984 Gee Pujser 41 Gene Pulser MXcell Cjang 1992 Vc
48. User Name Mike 15 Protocol CHO Press SAVE to continue Press BACK to return Gene Pulser MXcell 20 x 75 300 1 Home User Protocols ENTER 2 User Directories ENTER User Directories Registered Users no user registered no user registered no user registered no user registe
49. HOME DTE Home Protocol Set up Protocol Set up WHOLE PLATE 78 WELL SET 79 WHOLE PLATE 1 Home Protocol Set up ENTER 2 Plate 96 24 12 SHIRL ENTER 3 WHOLE PLATE ENTER
50. HErp Save User Name User Protocol Delete User Name User Pyooco7 Gene Pulser AXce 70 3 Clear ENTER 4 7
51. 14 Opt 12 well Exp Sqr Exponential wave Square wave 1 2 3 4 5 6 7 8 9 10 11 12 nm ND Uniform 96 well Exp Sqr 15 96 e ABCD1 6 EFHG1 6 250 350 uF 10002 ABCD7 12 EFHG7 12 250V 20ms 1000 uF 1000 Q 15 Uniform 96 well Exp Exponential wave Square wave 1 2 3 4 5 6 7 8 9 10 11 12 IJO Tn m o olw Uniform 24 well Exp Sqr 24 36 Uniform 96 well Exp Sgr Mixed Protocols Uniform 12 well Exp Sqr 72
52. x x gNrER ENTER Gene Pulser MXcell 2 Gene Pulser AXce Gene Pulser MXce e Gene Pulser MXcell 5 6 e Gene Pulser MXcell 8 e Gene Puser
53. P ujse Trac Pulse Trac 46 Pulse Trac Pulse Trac Pulse Trac 25 2 475 uF Gene Pulser MXcell 200 1 075 uF 20 10
54. In 1 7 droop t RC goyoop 0 RRC RE C C droop droop 43 Gene Pulser AXce T 44
55. 40 Gene Pulser MXcell 709 47 Gene Pulser AXce B 1 6 4 ABCD1 AB2
56. 26 1 save 2 Shift ENTER c1ear Mike 15 User Name Mike 15 Protocol Press ENTER to continue Press BACK to return 3
57. 7 7 PULSI E Eulsing Home Screen Gene Pulser MXcell Home oe
58. 7 12 well Exp 72 8 96 well Sqr 96 9 24well Sqr 24 10 12 well Sqr 72 Well set protocols 34 11 96 well Exp Vgrad Cgrad 96 12 96 well Sqr Vgrad Dgrad 96 29 Gene Pulser AXce Pre Set Protocol 30 6 pre set protocol
59. Pre Set Protocols 1 Opt mini 96 well Sqr Exp Opt mini 96 well Sqr Opt mini 96 well Exp Opt 96 well Sqr NP D 96 well Exp 24 well Exp more V ON tn 4 Wb amp Pre Set Pyofoco7 7 23 Gene Pulser MXcell 3 Save 26 o CLEAR Pre Set Protocol 26 o 4 save 26 o 5 PULSE
60. CD2 lk 2000 uF 96 100 pL 2 Gene Pulser MXcell Gee Pulser MXcell
61. 3Z 7xeg 96 well Exp Sgr 3Z Gene Pulser MMXce 6 Gene Pulser AXce 38 42
62. Gene Pulser MXcell Gene Pulser MXcell 1 Gene Pulser MXcell B71 1 Gene Pulser MXcell 2 d gt 2 Gene Pulser MXcell Gene Pulser MXcell 5 Home 3 1 Protocol Set up 2 Gradient Protocol 3 Us
63. Quadrant 12 Gene Pulser 7Xce 7Z Gene Pulser MXcell Gene Pulser MXcell Gene Pu ser 7x96 e Gene Pulser MXcell e e Optimization Bilt 2 Gene Pulser AXce
64. EFG 7 Quaoyan7 ABCD4 ABCD10 EFGH4 EFGH10 Gradient Protocol 4 FY 3 7 GRADIENT gradient protocol 1 Home HHD Gradient Protocol ENTER Plate 96 24 ENTER GRADIENT GRADIENT ENTER Plate 96 24 Program GRADIENT QUADRANT Exp soR
65. Reample gt gt Rec yz pc 42 PBS Gene Pulser MXcell Square Wave Pulse 44 77
66. LES Gene Pulser PBS Gene Puser
67. Op mini 96 well Sqr Exo 34 38 39 39 39 40 47 2 37 6 ppxC
68. gpo ag 0 5 5 x 106 cel mL 2 3 x 10 cel mL 1 5x 10 cell mL P gS Gene Pulser 7x 7 7 7 2 3x 70 7 mL siRNA 10 100 nM siRNA DNA 5 40 ug ml DL 96 7 100 200 uL
69. 2Z PresSeEProtoeo ER 28 Mimi Optimization Protocols eese eene tnnt natnra tns E 31 AU WEE uoc M 33 Well Set ProtoGols ic 223s Seed ee MI UE 34 Mixed RIOIOCOIS II 35 eee ene eneensnn enn nana 38 22st 38 9 42 vii Gene Pulser MXcell A Pulse Trac 46 Pulse Trac 46 Pulse Traci Br y JU zb V A ott aetate o io t e Pd 47 B 48 EA AORE bb 4 O 49 Ds 50 BEER PAPER 50 SU Fea Rete eet teach Rr EAE E LL EE DL can eae NDA d MM e PLE 51 Gene Pulser MMXce 1 Gene Pulser AXce
70. ENTER Yes Ao 3 ENTER Screen Intensity Delete Yes Mo Screen Intensity 1 HOME screen Screen Intensity 2 Use to
71. Gee Pulser AXce 15 15 e Gene Puser oe PULSE
72. 79 35C EC7070 Bio Rad Bio Rad A A Gene Pulser MXcell 500 210 J
73. oe 4 Home Protocol Set up o Gradient Protocol User Protocols Pre Se Protocols optimization 21 Last Pulse Data Management 700
74. C LE4 E AM7E ENTER 5 DELETE DELETE Save 26 PULSE PULSE 24 Last Pulse Pre Set Protocols Pre Set Protocols
75. pUrsE Edit well Set ENTER 20 5 Exe soR Save 26 o PULSE EUrsE 7 24 as Pulse WELL SET
76. Optimize Gene Pulser AXce 96 24 12 Gene Pulser MXcell 3 exponential waveform pulse square waveform pulse 500 WET
77. 165 2670JA Gene Pulser MXcell Gene Pulser MXcell Main Unit Gene Pulser MXcell Plate Gene Pulser MXcell 96well 7 165 2670JB Gene Pulser MXcell Single Shock Pod Gene Pulser MXcell Main Unit Gene Pulser MXcell Plate Single Shock Pod Gene Pulser MXcell 96wel 7 165 2671 Gene Pulser MXcell Main Unit 165 2672 Gene Pulser MXcell Plate 165 2673 Gene Pulser MXcell Single Shock Pod 165 2681 Gene Pulser MXcell 96we 11 amp 165 2681B05 Gene Pulser MXcell 96we 5 765 2682 Gene Pulser MXcell 24we 7 165 2682B05 Gene Pulser MXcell 24wel 5 165 2683 Gene Pulser MXcell 72we 7 165 2683B05 Gene Pulser MXcell 72we 5 165 2676 Gene Pujser 7 877 X 10 165 2677 Gene Pujser
78. siRNA 96 4 7 EIRA A BC 4 7 8 96 8 YD 12 Quadrant ABCD1 7 A B CEKU uus
79. Plate 96 24 12 Program WHOLE PLATE WELL SET 4 EXP SOR ENTER 5 Gee Pulser MXcell 18 Protocol Set up EF ENTER 96 Well Whole Plate Edit well Set press ENTER Waveform EXP SOR V C R clear SoR 96 Well Whole Plate Edit well Set press ENTER Waveform EXP SOR 500 500 5 500 3 S 10 0 P ODAS p
80. 38 3 PBS L CBS amp UR LET 1x 10 cell mL
81. Quadrant 96 24 ea 1 2 3 4 5 6 7 8 9 10 11 12 hs A T A s Quadrant 1 Quadrant 2 5 c c D D E E F adWant 3 Quadrant 4 F G i i IB G H H 7 7 9 96 24 Quadrant Gene Pulser MMXce 3 TI Gene Pulser AXce 74 16 pg o aga9
82. 72 Gene Pulser MXcell 2 Gee Pulser AXce
83. Protocol Set up Gradient Protocol User Protocols Last Pulse Data Management Screen Intensity Measurements 7 Home HOME Home Gee Puser Quagra77 Quadrant 2 Gene Pulser MXcell
84. pursEg 6 7 PorsE PULSE 7 24 as Pulse Gradient Protocol 20 Gradient Protocol
85. EN61326 EMC Class A O ass 4 lk Gene Pulser MXcell Gee Pulser MXcell
86. 10 Gene Pulser MXcell Gene Pulser MXcell 2 RER EXP SQR 25pF 25 2475 2 475 uF 50 7500 Gene
87. Opt mini 96well exp Square wave 1 2 3 4 5 6 7 8 9 10 11 12 Whole Plate Protocols Opt 96 well Sqr NP D 7 96 7 4 5B 250V 2 000 uF 10002 7 20ms 7 3 NP 10 Opt 96well Sqr NP D nm ND Whole Plate Protocols Whole Plate Protocols whole plate protocols 96 wells Exp 24 wells Exp 12 well Exp 96 well Sqr 24 well Sqr 12 well Sqr 250V 350 uF 1 0000 250V 350 uF 1 0000 250V 350 uF 1 000 250V 2 000 uF 1 0009 20ms 250V 2 000 uF 1 000 20ms 250V 2 000 uF 7 000602 20ms 33 Gene Pulser AXce
88. Quagra77 Quadrant Quadrant 7 7 96 4 7
89. 2 2 2 2 7 shift Shit a 2 7 b 2 2 c 2 3 Gene Pulser MXcell HOME Home BACK HELP HELPE
90. 24 7 500 800 uL 72 7 7 1 5 mL 6 4 ABCD1 AB2 CD2 C
91. ENTER QUADRANT QUADRANT ENTER Plate 96 24 Program GRADIENT QUADRANT Exp sor ENTER p PUISE 27 Gene Pulser MXcell 5 Quadrant Quagra77 Quadrant 6 PULSE
92. User Directory ENTER 2 User Protocols ENTER Protocols for user Mikel6 k E coli empty protocol empty protocol empty protocol empty protocol moreV Un Wh 3 Back Registered Users no user registered no user registered no user registered no user registered no user registered more V Cn 4 02 b2 Re Pre Set Protocols 4 save
93. T 40 EEN EDMIS TID KGVYAI4 A Vavld SESER RETH LIJI F DNA DNA DNA CsC DNA 5 40 ng 77 SiRNA siRNA siRNA MA SIRNA
94. x o m mlglolm gt 34 Mixed Protocols Mixed Protocols Opt 96 well Exp Sqr 73 95 COT 13 Opt 96 well Exp Sqr 1 2 3 4 5 6 7 8 9 10 11 12 gt v x m Tn m UW Opt 24 well Exp Sqr 24 35 Opt 96 well Exp Sqr 35 Gene Pulser AXce Pre Set Protocol 36 Opt 12 well Exp Sqr 74 7
95. 90 ml 57 BIO RAD 140 0002 2 2 24 TEL 03 6361 7000 FAX 03 5463 8480 532 0025 1 14 11 TEL 06 6308 6568 FAX 06 6308 3064 812 0093 2 5 28 TEL092 475 4856 FAX 092 475 4858 TEL 03 6404 0331 FAX 03 6404 0334 E Mail Life_ps_jp bio rad com MNL1652670 B 52
96. Pre Set Protocol Well Set Protocols Well Set protocols 96 96 well Exp Vgrad Cgrad 77 98 ABCD1 12 RER AV 350uF 10002 EFGH1 12 AC 250V 10002 11 96 well Exp Vgrad Cgrad x o m mlglolm gt 96 well Sqr Vgrad Dgrad 1212 96 ABCD1 lt 12 ER A 2000 uF 10002 20ms e EFGH1 12 AD 250V 2000 uF 1000 Q 12 96 well Sqr Vgrad Dgrad
97. CORT C 38 5 20 70 2
98. 1712 Gene Pulser MXcell 96 droop 200 33 4 29 17 96 droop 5 70 20 96 droop Q ms 20 3 34 7 14 14 6 200 33 4 71 4 146 1000 167 357 730 3500 585 1249 2556 77 V Voe TEF ECR ye O
99. Barsoum J Stable integration of vectors at high copy number for high level expression in animal cells in Methods in Molecular Biology vol 48 Nickoloff J A ed Humana Press Totowa NJ 225 1995 Change D C Chassy B M Saunders J A and Sowers A E eds Guide to Electroporation and Electrofusion Academic Press Inc San Diego 1992 Chu G Hayakawa H and Berg P Electroporation for the efficient transfection of mammalian cells with DNA Nuc Acids Res 15 1311 1987 Cregg J M and Russell K A Transformation in Methods in Molecular Biology vol 103 Higgins D R and Cregg J M eds Humana Press Totowa NJ 27 1998 Hui S W Effects of pulse length and strength on electroporation efficiency in Methods in Molecular Biology vol 48 Nickoloff J A ed Humana Press Totowa NJ 195 1995 Neumann E Kakorin S and Toensing K Principles of membrane electroporation and transport of macromolecules in Methods in Molecular Medicine vol 37 Jaroszeski M J Heller R and Gilbert R eds Humana Press Totowa NJ 1 2000 49 Gene Pulser AXce D Gene Pulser AXce 78
100. ENTER RreHT Gene Pulser MXcell X x Protocol Set up Protocol Set up x Y x Y przotoco1 Set up gt WHOLE PLATE protoco1 Set up WHOLE PLATE mWHorg PLATE WHOLE PLATE
101. Mixed protocols 35 13 Opt 96 well Exp Sqr 96 14 Opt 24 well Exp Sqr 24 15 Opt 12 well Exp Sqr 12 76 Uniform 96 well Exp Sqr 96 17 Uniform 24 well Exp Sgr 24 18 Uniform 12 well Exp Sqr 2
102. No 2Z Gene Pulser AXce Pre Set Protocol Gene Pulser AXce 5gjo ag Pre Set Protocol pre set protocol 3 8 12 24 96 well EXP SQR Y ms NP s e Grad gradient Exp exponential wave Sqr square wave 28
103. Gene Pulser MXcell Gee Pulser MXcell 6 2 Ge e Pulser MXcell P u se Trac 46 Bio Rad Laboratories a d Gene Pulser MXcell Home 70 0 4 Home ENTER
104. Pulser MXcell Gene Pujser 1000 W Gene Pulser MXcell 712 24 96 Gene Pujser 72 Quadrant Gene Expression Gateway www bio rad com genomics Gene Pulser MXcell V CF Q Ue ms e NP e
105. 350 750 uF 10002 7 Opt mini 96 Sqr Exp Square wave Exponential wave 1 2 3 4 5 6 7 8 9 10 11 12 250V 300V 250V 250V 1250V 2 000 uF 2 000 uF 350 uF 500 uF 750 uF 20ms 20ms 10000 10000 10000 IJO nim Oo Nn 31 Gene Pulser AXce Pre Set Protocol 32 Opt mini 96 well Sqr 8 96 ABCD1 lt 3 200 300V 2000 uF 10002 20ms ABCD4 6 250 2000 uF 10002 15 25ms 8 Opt mini 96well sqr Square wave 1 2 3 4 5 6 7 8 9 10 11 12 nm ON w gt Opt mini 96 well Exp X 9 96 1 3 200 300V 350 uF 7 000Q 4 6 250 200 500 uF 10002 RI
106. Gene Pulser MXcell 165 2670JA Copyright 2007 Bio Rad Laboratories Inc Bio Rad Laboratories Inc Gene Pulser MXcell I Bio Rad Laboratories nc Gene Pulser AXce Gene Pulser AXce Bio Rad EN61010
107. O gt 3ZC 24 Gene Pulser AXce LEE SIRNA siRNA GFP
108. er Protocols 4 Pre set Protocols 5 Last Pulse 6 Data Management 7 Screen Intensity 8 Measurements 3 Home Home 7Z Gene Pulser MXcell Gee Pujser Gene Pulser AXce 7 7
109. raise intensity Use to lower intensity Measurements Measurements Protocol Set up HOME screen 1 HOME screen Measurements 2 ID ENTER ENTER Measurements Well Sample Resistance Capacitance uF 25 Gene Pulser MXcell
110. red no user registered more V Un U Ne 20 5 3 nouserregistered ENTER 4 Shift H ENTER 5 Save 1 User Directory DELETE 2 Yes

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