取扱説明書 (Version 1.0) 研究用試薬 CLD01, CLD05
Contents
1. 2 7 Q amp A O RTS100 A O A2. RTS100 E coli HY kit ZE E 80 C E tRNA buffer 80 C 2 2 1 tRNA mam J 3 BR tRNA nm nm HiLyte Fluor 488 tRNA 497 525 CLDO1 CLDO5 TAMRA tRNA 546 575 CLDO2 CLD06 ATTO 633 tRNA 629 657 CLDO3 CLDO7 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 3 1 UAG N 2 20
3. UAG UAG N 20 20 3 N N UAG UAG N 20 20 REE 4 ProX tag ProX Gtag Figure 3 CloverDirect
4. CloverDirect N 1 Ee mU TET 2 1 CloverDirect NN 1 1 FRET 3 CloverDirect Bm tRNA 2 1 tRNA buffer
5. ProX tag UAG CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling E UAG mRNA 2 E UAG UAA UGA LELTE E C His tag tag 1 2 NRMD 20 3 N
6. 1 a 2 z DNase RNase HET CAD 80 2 UAG ProX tag E 1 N z GSSG 10 mM
7. 50 uL tRNA Chloramphenicol Acetyltransferase CAT page 10 Figure 5 RTS100 E coli HY kit UL RNase 5 DNA 100 ng pL DNA 100 ng uL o mRNA 8 ug uL 12 1 10 tRNA 5 E coli 12 Total 0 tRNA tRNA RNase tRNA buffer CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling Step1 tRNA 30 uL tRNA buffer
8. E tRNA RNase DNase E tRNA CloverDirect HiLyteFluor 488 tRNA Reagent for Site Directed Protein Labeling RTS100 E coli HY Kit 10 uL tRNA 1 uL X 10 uL
9. SDS PAGE FIOR 10uL TAMRA tRNA 1uL 1009 10 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling SDS PAGE Figure 6 1 0 251 IL SDS PAGE HiLyteF luor488 TAMRA 2UAG ProX tag 2UAG ProX tag CN f c N c c N c c N c c k lt E E S lt E x S o S o S oO x S o o 8 P 2 N o yP N Tae o xa 5 Ta 2 co Ta 2 CC a gt En Q ca 5 zeut gg 2 cn cn ae O nL SL I e e o olL Ow c Ss c S os
10. Figure 2 CloverDirect HiLyteFluor 488 tRNA Reagent for Site Directed Protein Labeling UAG UAG UAG 1 N page 11 Figure 6 2
11. mIkE NEHA 1 2 14 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 1 2 SDS PAGE 3 1
12. 15 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 8 E 1 1 MF20 nttD www olympus co jp Jp lisg genome m FRET 1 2 E E E SDS PAGE TR JL ARDD ND EI SDS PAGE 16 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 1 FRET analysis of protein conformational change through position specific incorporation of fluorescent amino acids Daisuke Kajihara Ryoji Abe Issei lijima Chie Komiyama Masahiko Sisido Takahiro Hohsaka Nature Methods 3 923 929 2006 2 47 2 124 12
13. N 20 FLAG tag 4 ProX tag UCU AAA CAA AUC GAA GUA AAC UAG UCU AAU GAG Ser Lys Gln Ile Glu Val Asn Xaa Ser Asn Glu Figure 4 UAG CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 5 1 E RTS100 E coli HY Kit UAG DNA DNA mRNA E tRNA 80C tRNA 80 E tRNA 10 80C 2 tRNA
14. N N i ii 2 ProX amp tag AUG UCU AAA CAA AUC GAA GUA AAC UAG UCU AAU GAG s35 Met Ser Lys Gln Ile Glu Val Asn Xaa Ser Asn Glu Figure 3 ProX amp tag Casel N ProX tag Case2 UAG UAG
15. CLDOl CLDO5 y ProteinExpress 6 CloverDirect M HiLyte Fluor M 488 tRNA Reagent for Site Directed Protein Labeling Version 1 0 mA mn Id EHO EIR ee 7RR UR E3 2 C5UGtEA DC RTS100 E coli HY kit URL http www proteinexpress co jp 260 0856 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mail tech proteinexpress co jp CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 2 2 EEA O e A FFF EFYDD EEF YT 3 3 Mi
16. His tag GE His SpinTrap Column m His SpinTrap Column GE Wash buffer 20 mM Phosphate buffer pH7 4 0 5 M NaCl 60 mM imidazole 0 1 Polyoxyethylene 23 Lauryl Ether Elute buffer 20 mM Phosphate buffer pH7 4 0 5 M NaCl 0 5 M imidazole 0 1 Polyoxyethylene 23 Lauryl Ether E SDS PAGE 12 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling Step1 His SpinTrap Column 2 mL 1 5 mL 1 Step2 70 100xg 1 S
17. UAG tRNA Figure 1 tRNA UAG Figure 2 UAG 100 N 20 N 2 20 phenylalanine XHiLyteFluor 488 ATTO633 Figure 1 tRNA mRNA Gl N tRNA UAG UAG
18. tRNA tRNA buffer Step2 RNase DNA DNA mRNA Step3 Step1 tRNA uL E coli 12 uL Step2 30 30 2 tRNA 80 C 2 AT Step4 SDS PAGE page 11 5 2 0 5 1 1 5 2 2 5 3 10uL TAMRA tRNA uL Figure 5 TAMRA tRNA TAMRA tRNA CloverDirect TAMRA Cat No CLDO2 CLD06 CAT
19. u O R an a re Y Y ANY 4 A EB a T DAE TT FYNFFRN YFWYR WRN YFWYR NF FAFFNFAY WYFINF D mm 8 Bl 8 5 2 RAY NJE OA TE eo 11 5 3 His tag iii 12 OE I 14 OA 15 SN 4 16 D 1 FYNYD NYFFRYN HEFY E E NF FWY REF FANAU 17 u n He YRR FE YE ERCH FFRF FRI FN FEN YY 17 11 Sc DUYCO BUY ARD U F si 17 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling CloverDirect tRNA Reagent for Site Directed Protein Labeling CloverDirect GFP 1 100 GFP
20. 8 2007 10 Ry S R tRNA Gn Ti HiLyte Fluor 488 tRNA 497 525 CLDO1 CLDO5 TAMRA tRNA 546 575 CLD02 CLDO6 ATTO 633 tRNA 629 657 CLD03 CLDO7 Biotin tRNA CLDO4 CLDO0S 11 URL http www proteinexpress co jp T260 0856 1 8 15 TEL 043 202 5755 FAX 043 202 5756 E mail tech proteinexDresS CO jD 1 CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling 18
21. tep3 500 uL Wash buffer 70 100xg 1 Step4 50 uL Wash buffer 350 uL Step5 10 70 100xg 1 Step6 500 uL Wash buffer 70 100 xg 1 3 Step7 2 mL Step8 Elute buffer 200 uL 10 70 100xg 1 Step9 Step8 Step10 Step8 9 X NaCl imidazole 13 6 CloverDirect M Mas Fluor 488 tRNA Reagent for Site Directed Protein Labeling
22. xX cC c S CG c Aa LL ao OL EF 90 6 LL am Pem PS gt i E mo mm uu P A ATTO633 2UAG ProX tag a EE 5 Figure 6 x gt S x gt z gt so E 8 3 8 8 2UAG AUG UAG A gt u amp o a o gt u ce o S g 5 E SHY lt T ProX tag ProX Gtag 0 25 uL HiLyteFluor488 488nm 520 nm TAMRA 532nm 580 nm a ATTO633 635nm 670 nm eS His tag i ee d sam gm ll CloverDirect HiLyte Fluor 488 tRNA Reagent for Site Directed Protein Labeling His tag
Download Pdf Manuals
Related Search