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取扱説明書(Supernova)

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Contents

1. 4 HRP 0 2 um
2. TBS T NaCl 100 350 mM Page 14 WESTAR Supernova instructionsXLS3 V1 0 11 WESTAR ECL SUN 100mL kit NN WESTAR EtaC WESTAR EtaC Ultra WESTAR Supernova WESTAR ECL SUN 50mL kit WESTAR WESTAR Nova 2011 50mL kit 2011 50mL WESTAR Nova 2011 50mLkit WESTAR Starter Kit WESTAKR EtaC 50mL 3 XLS025 0000 MSDS le WWW Cydr1ddeT1 CO T1 WESTAR Supernovainstructions XLS3 V1 0 Page 15
3. 4C TBS T NaCIl 100 350 mM WESTAKR ECL SUN instructions XLS063 Page 13
4. ij 2 Cathode iv 2 ij viii ix
5. ii TBS T 2 TBS T 3 bk 5 BSA WESTAR Supernova instructions XLS3 V1 0 Page 9 8 ij TBS
6. x 80 100V 30 60 15 25 V 20 30 xi xii V A 500mA node Te lm Fnam pad Fn Fter EL Emhrame R FIEW EE Foam pad lt VI Vi Page 8 WESTAR Supernova instructions XLS3 V1 0 6
7. a 1 1 fF Ma i CCD N Oe WESTAR Supernova instructions XLS3 V1 0 Page 11 AN AA AA N 10 TBS T Tweerr20 0 1 0 5 v v 5 lt
8. Page 12 WESTAR Supernova instructions XLS3V1 0 lt 10 kDa 1Ig TBS T NaCl
9. 4 3 5 TBS T VI a 1 bl HRP TBS T1 30 1 TBS T1 as 3 5 4 L 3 5 4 WESTAR SUPERNOVA 1 5000 1 100000 DM
10. 15 PVDF 30 15 15 kDa 0 45 u 15 kDa 0 2 lt 15kDa PVDF WESTAR Supernova instructions XLS3 V1 0 Page 5 i 4 ii
11. NH 0 NH O C NH Oo oN ON 3aminophthalate N hv enhancer Iuminal luminol radical aninn luminol endoperoxide WESTAR HRP WESTAR Kricka L J 2000 Methods Enzymol 305 370 390 Heindl D and Josel H P 1997 Non radioactive Analysis of Biomolecules 258 261 Springer Berlin Marzocchi E Grilli S Della Ciana L Prodi L Roda A and Mirasoli M 2008 Anal Biochem 377 189 194 Vdovenko M M Della Ciana L Sakharov 1 Yu 2009 Anal Biochemistry 392 54 58 Vdovenko M M Della Ciana L Sakharov 1 Yu 2010 Biotechnology Journa 5 8 886 90 Vdovenko M M Zubkov A V Kuznetsova G l Della Ciana L Kuzmina N S Sakharov 1 Yu 2010 mmunol Methods 362 1 2 127 130 4C
12. 7 KR 5528 103 0001 18 10 Tel 03 3661 4662 Fax 03 3661 0369 http www nmkkk co jp Cyanagen Via degli Stradelli Guelfi 40 C 40138 Bologna ltalia Tel Fax 39 051 534063 Email info cyanagen com VAT 02360111203 n REA 433637 10 000 00 Finance amp Technology 2497 bis cc VAT 02958121200 119 000 00 WESTAR US7803573 EP1962095 US7855287 EP1950207 US2012009603 A1 CA2742025 EP2405016 WESTAR Page 16 WESTAR Supernova instructions XLS3 V1 0
13. Te TBS T NaCl 100 350 mM NIR 53 BSA
14. 100ml A ImageQuant LAS 4000 Mini GE Healthcare MN DIAS II SERVA ChemiDocXRS VersaDoc BIO RAD A Chemilmager Alpha Innotech Image Station 2000 40000MM Kodak Odyssey 1L 10 100mL 1L 1L 10 200mL 1L TBS T 1L 10 TBS 100ml Tweenr20 1 mL 1L 5 BSA BSA V 5 g 1 TBS T 100ml 1L 10 S 100m 1L FOTO Analyst Luminary FXSystems Fotodyne LAS 3000 Fujiflm UVIchemi UVIprochemi UVItec Ltd G BOX GeneGnome Syngene FC L COR Page 6 WESTAR Supernova instructions XLS3 V1 0 3
15. 15 30 1 5 V CCD CCD 3 lt 5 1 5 A
16. 5 0 9 ii iii iv 10 v PVDF TBS T EA 2 Ce 7 30 60 ii 2
17. CO WESTAR MM WESTAR Supernova instructions XLS3 V1 0 Page 5 2 A B 1L 10x TRIS 250mM 30 3 g 1 9M 144 0 g SDS 19 w v 10 0 g 1L 1L 10x TRIS 250mM 30 3 g 1 9M 144 0 g 1L TBS T 1L 10x TRIS HCI 20mM 24 23 g NaCl 136mM 80 06 g 800mL pH 76 1M NaOH 1L 5 5g 1 TBS T 100ml 100mL 10x 1 0ml 0 59 S
18. 100mM 350mM BSA5 HRP SDS SDS SDS
19. feoel e ELPEI Su pernova _ Enhanced chemiluminescent substrates for Western Blot cA MIAA EAKI TL LT Cyanagen MM 100 60 Cyanagen Cyanagen ISO9001 EER ER Cert bal ran Page 2 VVESTAR SUupernova WWW CVdr1ddGT1 COTT1 LO 5 7 WESTAR 2 i 6 x V N_ NN hi
20. 8 10 9 11 CCD 10 1e co Ch Xx Ny lt JH NM Page 4 WESTAR Supernova instructions XLS3 V1 0 425 nm lt
21. 1 100000 1 500000 Page 10 WESTAR Supernova instructions XLS3 V1 0 9 ij ii 1 1 Westar ECL Sun 4 5 ii TBS T iv 1cm 0 1ml V 1
22. ii 1 2 4 TBS ij TBS T 4 3 5 TBS T iv TBS v 30 1 TBS T
23. SDS PAGE ii iv 2 vi Pre Stained Standard vii viii 90 130V_ 4 45 Se

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