取扱説明書 - 株式会社リプロセル
Contents
1. lt gt 1 Primate ES Cell Medium RCHEMD001 5 ng mL bFGF RCHEOT002 003 Primate ES Cell Medium 2 ReproFF2 Primate ES Cell Medium 3 Primate ES Cell Medium 2 2 ReproFF2 B2. ReproFF2 5ng mL bFGF RCHEOT002 003 ReproFF2 1 Dissociation Solutionfor human ES iPS Cells RCHETP002 Laminin 5 RCHEOT004 BD DMEM PBS CaTMgT free PBS 35 mm D 1000 A A1 Laminin 5 35mm 1 2 g Laminin 5 2 g A1 1 Laminin 5 4
3. Lamininh 5 B9 37C CO0 B10 ReproFF2 lg a Me 2 2 Mo Mo 3 Mo Mo 4 1 1 2mm MC Ci C1 A ReproFF2 1 5 mL Lamnin 5 PBS 2 ReproFF2 C2 ReproFF2 PBS 1mL
4. iPS Takahashi K et al Ce7 131 861 72 2007 pH 2 Mercaptoethanol iPS 3 4 ReproFF2 7 ReproFF2 ReproFF2 ES iPS
5. B2 ES iPS 35mm PBS 1 mL B3 1mL 37 C C0 5 B4 2 B5 ReproFF2 ES iPS 15 mL 3 B6 170xg 1 000 rpm 5 B7 ReproFF2 1mL p 1000 200 300um 4 B8 B1
6. A1 2 35mm 1mL PBS Laminin 5 vial PBS A1 3 Laminin 5 10 Laminin 5 A1 4 4 C 37 C 2 A1 5 4 A2 A2 1 A2 2 15mL A2 3 A2 1 15mL 3
7. 1 ES iPS bFGF 2 ES iPS ES iPS 3 ES iPS ES iPS 4 5 10 ES iPS Freezing Medium for human ES iPS Cells Feeder Cels MEF http www reprocell c
8. C3 1 mL 37C C0 5 C4 ReproFF2 15mL 170xg 1 000 rpm 5 C5 ReproFF2 1 mL p 1000 200 300gm C6 B1 Laminin 5 C7 37 C 596C0 C8
9. po 3 ReproCELL ReproFF2 Cat RCHEMDOO6 Dissocjation Solution for human ES iPS Cels RCHETP002 ReproFF2 4 ReproFF2 MC 20 C 2 8C 20C 2
10. 00 L A2 4 80C a A2 5 A2 6 DMEM 8 7mL 30 A2 7 30 35mm 0 75ml A2 8 3 4C WWW reDrocell com po 3 ReproCELL B ReproFF2 35 mm B1 A ReproFF2 1 5 mL Laminin 5 PBS 2 ReproFF2
11. om E mail info_ jp reprocell com WWW reDrocell com
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