Genova MK3 Life Science Analyzer 取扱説明書
Contents
1. 8 O 40 40 0000800 4100 ul U LI 028 U 0 L2. O IssDNA dsDNA RNA Bradford EP ET EE ET 1200000 000 O 199 9 00000 0 1 0 596 e 340nni O O 220nm 0000 1 000 00000 0 3000 1 999A 00000 0001 oO 0 002 30000 0 001A 0A 400nm 0000 3000 9999 00000 100 10 0 010 0 001 10910 MIL Ju g lll nhr 0o00 198 1000nm 00000 1nm 2nm zxO5nm 000000 5nme270nny 000000000 O 10 Ll 00 199 99 10000 9999 O00000000000000 0000000 1 999v0C RS2320000000 uuu 50 40 RH 800 115230vAC 200 50 60 u 50w 3650 x 2720 D x 1600 H nm 000 6kg
3. CAL KEY PRINT KEY 12 0 30 34 AIT TIME 08 DRTE 1 81 DATE FORMAT ts HELP TERT VES CELL CUVETTE EXIT 0000000000000 TIME DATE MM DD YYD HE D D DATE FORMAT DD MM YYDO 0 0 0 HELP TEXT 2
4. Lowry 1 0 0 O Lowry O Biuret 0 0 30 320000000000 TESTS SYSTEM TEST FASS Ensure sample DARK CAL PASS chamber is gmFL3 Do not WHUELEHGTH CAL door during on teztz CRITICAL ERROR CALIBRATION DATA FAILURE SYSTEM ERROR OPERATING PARAMETERS FAILURE
5. SYSTEM ERROR DARK LEVEL CALIBRATION FAILURE 00000000 000060 Optical Alignment 0 0 SYSTEM ERROR WAVELENGTH CALIBRATION FAILUER 1 000 11 Genova PHOTOMETRICS DHR7FHR MODE FRO TEIH MODE IHSTRUMEHT SETUP SECURITY 33 0000000 UP DOWN KEYS LEFT RIGHT KEYS
6. B C A second order curve fit quadratic 562nm Bradford second order curve fit quadratic 5900 0 595nm Lowry second order curve fit quadratic 550 750nnij 0 500 750nm Biuret first order curve fit quadratic 5400 0 550nm Direct UV I B DH 10 B C A Bicinchoninine 0 00 00 0 Bradford 0 0 2 Biuret 0000 0 rewyggaggagagagagaomnsuuggggaggauuu 10
7. 1 2 u 3 4 6 L a 00 000000000000000000000000000000000 001
8. LIGHT LEVEL 5 20 0000000000000600 ul ul Ll L 001 OO LO 1 000000000000000000000000000000000 2 3 33 1 0000000000000 400000600 2 3 2 4 5 e O 50 0 50 00000000000 51000000000000 636 041 00000000000000 0000 035 134 0000000000 1000 0 2000 9o0nni 060 377 0000000100000 03
9. Y mhi ng n ng nh ng 1 0000000000000000 0 0 10g 0 010 0 01 0 30 Ferform CAL first please EXIT SETUP 000000 260 280nr 0 0 O0 O 260 230m 0 d ab BO 7 U U CAL 2 CAL zB8Bnn RATIO RATIO EXIT SETUP EXIT SETUP 320 3 3 CAL 2 A 26H 288 B nmgaHbs 1 RATIO A 326 RATIO B EXIT SETUP EXIT SETUP 25 Genova 000000000000 2 3000000 MEASURE 26 MEASURE zSBnm MEASURE
10. FACTOR 000 Factorx 50 8 0 0 0000000000000 0000006060 UNITS lUUDDDUDUUDUDUDUUDUDUUUDUUDUDUUDUUDJUDUDUUDUUUUDUUuuDnun ng hp RESOLUTION ao ij 0 01 0 00111 11111 WAVELENGTH 0 081 830 EsIT SETUP 3l 32 3 9 GOOD PRACTICE GUIDELINES
11. LU ELE D 6H 0 T 11 230vAq 20 06 1 5 L ui ER IN 001 001 ui 2 W L L 0 L 2 1 5 L L L 3 3 20 230000000 xa A z
12. 280 0000000000 260 00000000000 5500 600 D OBL EL 1 dsDNA 50 g nh ssDNA 37u gm0 RNA 40p 30u g nh 280m 260 2 000 18100000 RNATT CI U U D 2 0 00 230nnd 22000000 3m 00 UU U C C UO 7 C C U 7 7X 7 C O 7 7U 7U U 7 U U
13. 3600000001 000000000 PROTEIN B U aan B C H BRRDF ORC LOWE BIURET DIRECT Llu D B C A Bradford Lowry 0000 0 0 0 000000000000 0000 curve fit Quadratic SETUP B1 c B DHL BO DU UB V C D 0 D UI UI U I EXIT WRUELEHGTH UNITS RESULLT IUOH EXIT WAVELENGTH 00 B C A 562nm Bradford 590nm Lowry 750nm Biuret 540nm 0 30 UNITS yn ng mhi ng nh ny d T RESOLUTION 0 000000000000000 000 10 10 0 010 0 01000000 0000 E IT VIEW METHOD STDS Curve can be calibrated bu Frezzing the CAL EXIT VIEW 1 9 001 001 NO OF ST
14. L ASCII D or d ASCII T lt CR gt 540 ASCII A lt CR gt 540 ASCII C lt CR gt 4 540 ASCII V lt CR gt 5 5 540 ASCII 5 5 G540 540nni 000000 O F10000 10000000000 ASCII SC lt CR gt ASCII SO lt CR gt 1000000 9
15. ernova E 268 288nm 268 z238nm PURITw SCRH dzDHH zzbHH IGO URFIRBLE 260 280nm 260 230 260 280 0 0 0 O 26 230md 23 24 250 RATIO RERD 1 FACTOR2 DILUTION CORRECTION WAVELENGTH UNITS RESOLUTION ExIT FACTOR 1 62 FRCTOR 2 26 DILLTIDH CORRECTION H 320 RESOLUTION 1 Abs Abs 2300 000 2801 000000000 260nmi 000000000 8
16. amp _ 00577 0 057 7 1140 EsIT SETUP 5 WIT FACTOR 1 888 UNITS PPM RESOLUTION B 1 WRUELERGTH 260 5 UNITS RESOLUTION 0 0010 3 uiui WAVELENGTH 15 16 00000
17. ASCII lt gt Li DL D HL UO UL D HELD UU ASCII Fxxxx x lt CR gt L 000 36 Het 6 2 RS232 00000 000 2 TXD2 RXD3 00000000001 RTS 4 5 55 RTSJUDD DSR DCD8 DTR20 IBM XT D SUB25 TXD 2 2TXD RXD 3 e 3RXDUPCHD RTS4 e A4RTSDHPCDU UD CTS 5 e 5CTSU PCI D DSR 6 e 6DSRUPCUI DCD8 e 8 DOCD PCII DTR 20 e 20 GND 7 e 7 GND IBM XT D SUB9 TXD 2 e 3 TXDJ PCHUD RXD 3 e 2RXD PCHI RTS4 e 7RTSUPCHDH CTS 5 e 8 DSR 6 e 6 0 DCD 8 e 1 DCDOPCOO DTR 20 e 4 DTRODPCOOO GND 7 e 5GND 542 009 3 38 63 00000000000 inv D EC D DD EN61326 1998
18. 1 260 WAVELENGTH2 D 230 CORRECTION 5 WAVELENGTH 0000 0 50 0000000 0000000006060 UNITS gY hO ng mhi ng hgg ul U gd RESOLUTION 0 010 0 000 PURITY SCAN 1 2500 90 50n l 00000 O 0000000000000 PURITY SCAN 000000 AIT SETUF 27 Genova Remember to Ferform blank calibration SETUF ERIT WAVELENGTH CRHLIBRRTIHG EsIT SETUF
19. bd EB 1265 bx 8 12113 B B1723 19 Genova 00000 0000000000 4 BL RES 562 SETUP CURVE 161 0 180 ob2 SETUP CURVE 20 0 30 3 7 Direct UV 2800 260 0 0000000000 80x 1 55 260 0 76000000000000 280 0000000000 0 D D Factor 1 1 D U LU LI C D 1 55010 Factor2 0 000 000000 EsIT REAL AIT FRCTOR 1 1 55B8ETB FRCTOR 2 B r6BE 6 DILUTIOM BiBm asgar RESOLUTIOH 1 F
20. Q M i 1 UP DOWN KEYS 2 LEFT RIGHT KEYS 3 ENTER KEY 4 CAL KEY ugggggggggggggggggggugugu 90 5 PRINT KEY 2400 00 1 ROCKER SWITCH 2 POWER SOCKET 3 OUTPUT SOCKET 4 OUTPUT SOCKET 000 ggag udg RS232 0 25 0 30 0 3 310000 00000000 1980 100r 000000000000000000000000000 UU
21. 00000 CELL 13 14 SECURITY z IT IHSTRUMEHT LOCK SECURITY CODE 001 EXIT INSTRUMENT LOCK 00000 INSTRUMENT LOCKO INSTRUMENT LOCK Y ES O INSTRUMENT 50 00 00 999000001 FASS CODE FHOTOMETRICS DHR7FHR MODE MODE IHSTRUMEHT SETUP SECURITY SECURITY CODE 999 Enter 0 30 35000000 000000000
22. Y SCHHHIHG ERIT SETUF 28 0 30 456rnm ERIT SETUF Peak 39 1 458 ERIT SETUF dsDNA ssDNA RNA and OLIGO MODES _ RBS 1 T ERIT SETUP 29 Genova 30 SETUP 000000 450 EXIT DILUTION 1 9 1 FACTOR SB B LIHITS FESOLLITIOH 1 WAVELEHGTH ssDNA EXIT DILUTION 2 8 1 FACTOR 37 B888E 8 UNITS RESDLUTIOM 1 WAVELENGTH pPa ml EXIT DILUTION 1 2 1 FACTOR 48 UNITS RESOLUTION 1 WRUELEHGTH pa ml EXIT DILUTION 1 1 FACTOR 38 8 LIHITS 1 WAVELEHGTH pa ml 0 30 EXIT DILUTION
23. ntes uidet voe d DECR Re eet em be s 35 TITEL T 35 H60 nar eC eed ane dr ex a 36 6 Je ppp RE PS e noa e ze rtt eon are zd 36 652 RS292 D b Bp E iR Perth ai ua u t Rue m Et Rte Pate 37 s da uM NE E 38 ECT e bees ac aku d E UU OT CR AS GR DRE A CA 38 SS CR C CURA Do du D RR PR A CR ca Ree Ce 0 8 39 1 N 3 4 5 010 1 1
24. 2 320 RATIO A 32H RATIO 326 RATIO EXIT SETUP EXIT SETUP EXIT SETUP D 0 504 266 290 B 1HSHbs B 194BHbs 326 RATIO B a34Hbs B 55 EsIT REAL SETUF VARIABLE RATIO 0000000000 260 28000 260 230 I 00000000000000 UU Uu 2ey280nm 0 0 260 230 FRHCTOR 1 43 1gBE FACTOR 2 2 420E 4 DILUTION Ba2mramaar hIRUUELEHGTH 1 ze WARUELEHGTH 2 CORRECT IOH WAVELEHGTH UHITS RESOLUTIOH 26 0 30 EXIT 0000000000000 1 Abs wavelength 0000000000 FACTOR2 Abs wavelength2 000000000 3 DILUTION Enter
25. 7162 0805 H TEL 03 3235 06610 9 FAX 03 3235 0669 T 532 0005 rp JM p 2 TEL 06 6396 0501 FAX 06 6395 2588 1812 0054 fW rt x PCS 2 zB TEL 092 631 1012 FAX 092 641 1285 gt k 2 2 D 3 O Hud k ko HELIER ERTH EL 9
26. EN61010 1 1993 EMC Directive 89 336 EEC Low Voltage Directive 73 23 EEC Martyn J Fall Managing Director Jenway Limited Gransmore Green Felsted Dunmow Essex CM6 3LB England 0000 Lu uut 0o00 00000 Lowry BCA Bradford Biuret 260 280 0000 260 230 Direct UV DNA Lowry O H Rosebrough N J Farr A L and Randall R J 1951 J 1951 J Biol Chem 193 265 275 Smith P K Krohn R I Hermanson G T Mallia A K Gartner F H Provenzano M D Fujimoto E K Goeke N M Olson B J and Klenk D C 1985 Anal Biochem 150 76 85 Bradford M 1976 Anal Biochem 72 248 254 Ohnishi S t Barr J K Anal Biochem 86 193 1978 Layne E 1951 Methods in Enzymology 3 447 454 Groves W E Davis F C and Sells B H 1968 Anal Biochem 22 195 209 Warburg O Christian W 1941 Biochem Z 310 384 Kalb V F Bernlohr R W 1977 Anal Biochem 82 362 371 Maniatis T Fritch E F Sambrook J 1989 Molecular Cloning A Laboratory Handbook 3 39 Genova Hukum 1JEN 0001 1 2003 4 1 1 636 015 CN2420 REV C 01 02 2004 4 2hk 636 015 CN2420 REV C 01 02 ILIKKA AA RI X 1 i Bu a sc dico FEBR
27. 5 12 220 900 1000 0 500p 1D 632 001 000000 0 230v0 0 632 031 0 0 000000 115V 634 001 400000000000 021 041 630 020 1 Bm 630 021 0000000000000 251 630 022 000000000000 26 630 005 200 100mm 00000 035 079 100x 10nmil 1 0 D f 035 087 50x 10mm 00000 035 029 40x 10mm 035 086 20x 0 0000 035 027 10x 100m 060 087 000000000000 000 0 3200 1100 1000 0 0 1m0 060 084 O0 000000000 0 3200 1100 1000 0 3 5r D 060 229 p0 000000000 0 3200 1100 5000000 3 5m 630 028 800000 52000000 012 094 000000000000 630 004 10x 10mi 00000 016 021 0000000 2A 35 0 60 61000000000000 5232 1200 7 1 25
28. ACTOR1 Abs 280a 000000000 FACTOR2 Abs 260nni II 0000000 3 0000000000000600 DILUTION 0001 UNITS ul H g nh ng nh ng hg ng gy d ny d T RESOLUTION 0 1 0 011 21 Genova Ferform CAL first please EXIT SETUP 2800 260nni 2 EXIT SETUP EXIT SETUP 20H B amBaHbs EXIT SETUP 22 0 30 280m MEASURE 2 MEASURE z Benm EXIT SETUP EXIT SETUP 0 2017 250 B lrv2Hbs EXIT SETUP 3 8 DNA RNA 00 000000000
29. D NO ppm ABS 3 6 1 Cenova EXIT Fress CAL to zero absorbance Sample blank EAIT Place stg 1 in the sample chamber and Fress CAL HIT THOD HO STDS Fen 3 2 4 5 6 B 38a Ae m e pon Dux 0 30 Place std z in the sample chamber and Fress CHL STATISTICS 5 ax24bpx4c 0 0 STATISTICS 000
30. Genova MK3 Life Science Analyzer 636 015 CN2420 REV 01 02 QD aac afi e 8 3 Eh Pp PPD ga A EE RUE 4 TTE NUPTAM LT 4 PM 4 DI XE 222222 D el 6 6 PARAR ESTE uM WM 6 223 Fa r BEES accede bach ope eb dns 7 2 4 HI Dae S a e tcv ut eese La eoa S E ed 8 FI SE aa CR Oe ac C OE EN CR ADR id 9 9 EROR Paka qa 11 J EET sau ana una QS 12 anus 13 15 3 6 g El EJ E C ER a Ta 16 21 3 0 DNA RNA TH ee E SES CEU a a ah 23 3 9 GOOD PRACTICE GUIDELINES 32 paci l l ri e e QU M de Vd vk Qi CR u de s 33 Zappos tit ac oto aciei EIE AS 33 4 2 EE Blob B e t tasca ul aap ugat One a cce Oct oe e Ea 33 35 S Ln
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