Home

bio-optica - vtp300 histology vacuum tissue processor user manual

1. Ji 06 00 i BATON 7 r y 000 4 d d Cassetenr lt MOE SAMPLES 000 j y 3 01 FORMALIN 0000 0 A 0 EOP Time 223414 02 WATER 00 0 0 A 0 EOP Day Wednesday 03 ALCOHOL95 0000 0 A o 2 04 ALCOHOL 95 00 0 A 0 Delay OO days 00 hours 00 min 04 ALCOHOL95 0000 0 A Y E 05 ALCOHOL 100 00 00 0 A 0 RESET 06 ALCOHOL100 0200 0 A O0 07 L100 00 00 0 A o 08 XYLENE 0000 0 A o d SIE 0000 0 A 0 a 10 XYLENE 0200 0 A 0 j Y WAX 60 0000 0 A 0 WAX 60 0000 O 0 Caution special protocol REVERSED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it A Reagent Management System in a tissue processor is necessary to Optimize the reagents utilization Avoid the not recommendable bulk substitution altogether once a week for example Awoid boring and not reliable paper records Avoid the manual movements of tanks to prepare the instrument for the next process for example by manually substituting the dirtiest tank of a group of reagents and shifting the other tanks backward Oooo In the VTP300 the usage of a good RMS is much more important given that as explained in other chapters the instrument can be used for fast and for slow processes with a di
2. CSO Rev 120416 of 16 04 12 Page 7 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it O Severe damage can result if the instrument is connected to a power supply different from the rating stated in the identification tag placed on the rear of the instrument O The instrument must NEVER be used without being connected to an appropriate and fully efficient ground connection O f damages due to transport occur DO NOT use and DO NOT connect the instrument to a power source Contact our technical service O This instrument has been designed to work 24 7 for this reason and for operative precautions the power switch is placed on the rear of the instrument O Access to the instrument s internal components is reserved only to specialists trained in the service of the instrument Always disconnect the processor from the electrical main source before accessing the electronics and internal parts O BEFORE replacing fuses disconnect the instrument from the power source O Always make certain to correctly engage the reagent bottles DO NOT open the processing chamber lid when the instrument is working without following the instructions contained in this manual Use specific precautions in han
3. LOLOL LET LLL rr a rio 003 55 11 41 15 36 3 les ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it There are two types of alarms Alarms 1 through 50 are NOT BLOCKING ALERTS NOT BLOCKING alerts are only WARNINGS and do not interrupt the program execution because the cause of them is not critical for the process completion In this situation a program stoppage may produce worsen results than its continuation When an ALERT occurs the program continues and the screen displays the alert number and a brief explanation of it Upon the process program completion the cause of the alert can be determined and usually easily eliminated by the operator Procedures listed below for each type of alert alarm should be followed to correct the cause of the problem If the problem persists a call to our service department may be indispensable Alarms 51 through 99 are BLOCKING ALARMS These are true alarms and the process cannot continue as the cause of the alarm makes it impossible to proceed In the event of a BLOCKING alarm follow the instructions displayed on the screen to 1 Stop the acoustic alarm 2 Try to empty the SPC from wax reagent 3 Remove
4. Page 35 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilita 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The most common processing program has a stationing time of one hour for each step bottle Is suitable for the vast majority of histology samples moderate variations on the timing are sometimes adopted in relationship to specific needs and the kind of reagent used For example a The xylene substitutes may require a time longer than the xylene a Higher reagent temperature allows shorter timing a Also the use of vacuum may allows shorter timing The pressurization or de pressurization of the SPC allows a better sample infiltration Normally the use of vacuum de pressurization is sufficient to obtain a good infiltration for most of the samples and it is advisable in each station except for the formalin and the first wax The combined use of vacuum and pressure should be limited to the processing of very difficult samples big and or fatty The usage of P V with normal samples other than uselessly expend the processor components may cause undesired samples over processing The reagent heating in the SPC up to the maximum allowed temperature 45 C is advisable starting from the 2 3 reagents before the waxes to prepare the samples to the thermal
5. This alert is issued only in unit with the RS 232 connected UPS CAUSE The process has been suspended because during a power failure the UPS batteries went below 4096 charge The process is suspended to avoid that the energy would be completely lost during a drain or fill action with the risk that the sample will remain in air The instrument is set in a condition of power saving to increase the UPS batteries life SOLUTION Restore the electrical power if the power is restored before the batteries would be completely exhausted the process will be automatically restarted Any case the process will be restarted after the power is restored even in case of total loss of batteries charge but in case of wax steps a delay is introduced to ensure the wax complete melting If the electrical power is not restored within 5 minutes from the issuing of this alert the condition is switched to alert 23 and the instrument prepare itself for the complete switching OFF see alert 23 23 PROC SUSP INDEF EXPLANATION The process has been indefinitely suspended due to the extended opening of SPC lid or lack of power when the instrument is equipped with the UPS and the batteries are too low see alert 22 CAUSE In case of suspension see alert 20 and 21 the process must be restored within 3 minutes over that limit the suspension is considered unwanted or accidental and this alert is issued to not mistakenly confuse the situation with the temporary s
6. UPS User Manual optional The control software of this processor may be subject of modifications that may not result in visible differences from previous versions BIO OPTICA MILANO SPA VIA S Faustino 58 20134 MILANO Tel 39 02 2127131 Fax 39 02 2153000 Web www bio optica it esa cso Rev 120416 of 16 04 12 hs Page 60 of 60 MED ISO 9001 ISO 13485
7. CS E Rev 120416 of 16 04 12 Q Page 26 of 60 MED ISO 9001 ISO 13485 Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 o Optica End of process 3 Tank Processed Cassettes DAF regressive counter 8 300 300 9 300 180 10 300 120 At this point the RMS will require the substitution of tank 8 because its DAF has reached the predefined limit of processed cassettes after the substitution the counters of this tank will be reset In the next process the tank number 8 will be the last in the group and at the end of the process the counters will be as follow End of process 4 Tank Processed Cassettes DAF regressive counter 9 400 280 10 400 180 8 100 40 After process 4 the RMS will not require any substitution because none of the counter reached or exceeded the limit End of process 5 Tank Processed Cassettes DAF regressive counter 9 500 380 10 500 240 8 200 80 After process 5 the RMS will require the substitution of tank 9 because its DAF has exceeded the limit End of process 6 Tank Processed Cassettes DAF regressive counter 10 600 340 8 300 140 9 100 40 And so on The sequence here shown typical of a long overnight pr
8. 20134 Milano E O fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilita 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it Before using the instrument please carefully read this manual Please pay particular attention to the precautions that must be taken for user and product safety To avoid potential instrument damage do not use reagents different from those specified in this manual The warranty applies only if the instrument is used in the correct manner and in accordance with the information and advice provided herein The manufacturer declines all responsibility for possible damages to persons and or objects due to improper or inexperienced use of the instrument This instrument has been designed to be solely used in a Histology laboratory by on purpose trained technicians for processing specimen provided that is used accordingly with the instructions contained in this manual The VTP300 is a tissue processor designed for the following laboratory applications gt fixation gt dehydration gt paraffin wax infiltration of histological tissue samples Any other usage is expressly prohibited Failure to follow to these instructions may result in accidents damages to the instrument and accessory equipment personal injury esa CSO Rev 120416 of 16 04 12 hs Page 6 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 2
9. 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i The VTP300 can perform short step time processes Reduced step times can be adopted for biopsies or small size samples The user will have to determine the best step timing basing his choices on past experiences and also taking care of what follow 1 The efficiency of a vacuum tissue processor like the VTP300 is superior to that of a traditional carousel tissue processor 2 The shorter are the step time the more is important the overall quality of the reagents and their correct maintenance 3 The time indicated for each step program is inclusive of the fill and drain times normally 1 15 forthe filling 230 for the drain for processing programs with a total time gt 5 hours 115 for the drain for processing programs with a total time lt 5 hours 4 For the correct infiltration of small samples it is not strictly necessary the use of 4 waxes 3 would be sufficient it is advisable to jump the second wax 5 The minimum step time is 5 minutes We recommend to not set short times in the first wax The minimum time for it must not be shorter than 20 minutes That is due as already explained above to the contact of the first wax with samples and baskets that are still quite cold In the following wax steps this problem doesn t exist anymore If the VTP300 is asked to drain the first wax
10. 39 02 21 53 000 www bio optica it The purge reagents are also under the supervision of the RMS It is therefore sufficient that the RMS is active to have it recommend the substitution of the purge reagents every 3 to 9 processes The recommended numbers of processes for the substitution of the purge reagents are as follows Cleaning only the SPC 9 processes Cleaning the SPC and the baskets 5 processes The aforementioned quantity is valid for correct use of the instrument It is however contingent upon the manual removal of as much residual paraffin as possible from the lid and SPC before beginning the purge cycle Please note if the user desires to replace the purge reagents before their counter reaches the preset limit it will be necessary to reset the counter by the Zero Resetting Single Counter function The charcoal filters are also administered by the RMS In addition to the percentage indicator visible on the Main menu with 096 representing a new filter and 10096 representing an expended filter when the filters reach or exceed their preset limits the RMS will prompt the user to replace them at the same time he is prompted to replace reagents The charcoal filters should be replaced between a minimum of 60 and a maximum of 120 processes based upon the following factors Without the use of the WCC the filters should be replaced every 90 to 120 processes With the use of the WCC the filters should be replaced every 60 to
11. 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Although explained later in more detail basically following the purge cycle and the WCC if activated the system will automatically prompt the user to replace the reagents that have reached their pre determined processing limit Defining reagents allows the operator to identify the type of reagent that is used in any single tank The labels to be used here must be prepared in advance in the instrument SETUP It is important to notice that the assignment of a reagent name to a tank in the RMS automatically updates the reagent names in the process programs However it is important that the reagent label corresponds exactly to the content of the tank defined during the setup of the RMS This is due to the fact that later when the RMS will prompt the user to replace a tank it will suggest the reagent that needs to be filled in the tank accordingly with what programmed here if the label is not consistent with the reality a mistake can be easily made For example if the reagent label Paraffin is assigned to the tank in position 4 which is not located in the wax chamber later when the RMS prompts the user to replace tank 4 the user will be instructed to put Paraffin in that position Clearly this would be an error Therefore it is important to carefully plan the entire reagent selection process prior to setting up the R
12. 90 processes These values are somewhat approximate because can vary due to Environmental factors humidity temperature The duration of the processes The application of the vacuum or pressure on all the steps of process The frequency of agitation of the reagents If the VTP300 is operated in an area with high relative humidity if pressure and or vacuum is requested for most or all of the process steps if the reagents mixing in the SPC is set to the maximum frequency it will be necessary to set the limits for the charcoal filters substitution at or below the minimum values mentioned above Important Note If the charcoal filters are changed before the process counter reaches the preset limit it will be necessary to reset the counter with the RMS Single Counter Reset function on the RMS Setup menu The activation de activation of the WCC takes place on the RMS Setup menu De activation of the WCC is advisable when e An excessive consumption of the charcoal filters makes its use less advantageous than the paraffin savings This may occur due to a combination of the environmental and usage factors mentioned above e The instrument is malfunctioning frequently Discontinuing use of the WCC will lower the workload for the VTP300 and may eliminate one possible source of malfunction eso cso Rev 120416 of 16 04 12 hs Page 29 of 60 MED ISO 9001 ISO 13485 If for any reasons the WCC has been interrupted or not allowed to
13. Dern tetuer exe Eee c FERE Ye EUR Cr deae dalia 43 1 PW FAILURE ted 43 2 DIFFICULT DRAIN cocoa AA AA AA 44 3 SHORT DRA Nicosia da alare 44 4 PIV TIMEOUT m 44 9 FULL HM sona ii E 44 6 ABORTED STEP cia a aa ai bas 44 csQ Rev 120416 of 16 04 12 Page 3 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i f MIEZenmuelsm 44 B LONG DRAIN ET 44 9 WWC TEMP LOW a aida need 44 10 PNY TIMEOUT ah e ere nce te re tete eee aec ete ee te cuc de tere ee eee ce EE ote vede eu 45 RENE JIMENEZ uA 45 12 5 MZ TEAIEBRE 5e eteno eret ce teet eA UN AAT e did 45 139 VR MSW EAILURE ie Rede EI i 45 14 SPG TEMP EOW tree tee eee eue e tiere a 45 155 SHOR TRIVE t idees teet iet et dL e A ect 45 205pnGnoc SUSPUSERA V aaa 46 2i PROC SUSP LID Lala 46 22 PROCSUSP UPS liana A 46 23 PROC SUSPINDER 00 dedican 46 30 RMS POSTPONE D aii A aa 46 32 RMS POS TPON PW ui a it ee ea add 47 34 WRONG PASSWORD ii 47 40 ELODEV LACK cui 47 ALARMSABLOCKINO isso tdi tddi 48 X ES IBI ORO UBI m TE 48 52 FILETIME OU Titi ii ile 48 53
14. LINE CLOGGED unicidad 48 54s SPCOVERTEMP sucia aliada 48 55 TS SPC OUT aia dida ri 48 56 SHORT FIL cuna ill a 48 DE MR OUT rianne ia ala a ra 48 58 FILE NOT FOUND cia ela ii Rail 48 59 SPC TEMP LOW sanci n iia 49 60 WWG TEMP LOW nenoaii A aaa 49 61 WWC OVERTEMP enone rn ta dl a E E Pe ea a E E e ee eee due ae eod 49 62 TS WWODU TT cresta dat tt bad a 49 63 PRESSUREJNNR a usi iaia 49 64 DRAIN NO PRESOS nr add 49 65 DRAINCTIME OL T cire rete Etre t rre tette reete eoe nete reet be deret ce ti 49 66 SPC NOTJEMEP TC oct ccrte eee teer ee eroe t ferentes nd ted din 49 sra OVER ELL 49 70 89 COMERRORS iii 50 90 gt 93 VO ALARMS cccccece cece cece cee ceeececeauea ee seeeeeseaeaaeeeeseeesueeaueaseeseeeeaueaseseeeeeseaeesaeaaateeeeeseanas 50 Alarms historical archilVe inert deret aaa 50 Service first aid instrument assistanCe cnc eee eet nn ee nano rn ee naar rr naar e ne nn cn cnn nennen 50 TouchiS Calibfi ill lla aida 50 Drain SPO arenan lalla lei 50 VACUNA Em 51 Presses dia A iio 51 EU TES anali ARA A i ai 51 SPGiHeatngi O NN 51 Alans Pl iio iodo ari 51 lB iNIcIH Lo RR 52 Acci 52 Alarms FIesel ederet EE EEE ii 52 Service Menu abbreviation liSt 1 iaia ii 52 Semice ASSIS INCE all 53 Safety e c LT 53 Protecti
15. after a successful purge it is set to 0 SETP SPC SPC temperature setpoint TEMP SPC SPC temperature T MAX SPC SPC maximum temperature reached since the last reset SETP WWC WWC temperature setpoint TEMP WWC WWC temperature T MAX WWC WWC maximum temperature reached since the last reset se Rev 120416 of 16 04 12 v hs Page 52 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O pe i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilita 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it Before calling our technical service please collect the following information Type of instrument a Serial number a Software release a The number of any alarm that has occurred O Please provide this information to technical service upon contact The thermostat controls include a maximum temperature cut off switch to prevent overheating of WWC and SPC These devices do not prevent alarms but prevent overheating when primary control devices fail Do NOT use the instrument when an alarm indicates an abnormal temperature status In the event of an overheating it is advisable to switch off the instrument and disconnect the power cord In the VTP300 there is no danger of excessive pressures The pressure levels developed in the SPC are small and do not present any hazard Sometimes a light pressure can be present in
16. be transformed in 1 hour and 59 minutes but the number 160 will be transformed in 2 hours 1 hour 60 minutes 2 hours The number 1099 will be transformed in 11 hours and 39 minutes and so on Initially this particularity may appear cumbersome but by respecting the principle of not entering more than 59 at the end of the time string nothing unexpected will happen If a time of 0 hours and 0 minutes is set the step will be ignored even if the other parameters temperature and vacuum are set The station time includes the reagent filling and draining times approx 3 minutes The selectable reagent temperature range is 0 for ambient from 20 45 C The selectable paraffin temperature range is 52 65 C The Pressure Vacuum field allows the selection of the following options A ambient pressure V vacuum SPC pressure will be lowered to 600 HPa below ambient P pressure SPC will be pressurized up to 200 HPa above the ambient P V an alternating cycle with an 8 minute frequency of pressure and vacuum Varying the pressure in the processing chamber facilitates reagent infiltration into tissue specimens The following P V settings are recommended e Biopsies Vacuum or ambient pressure e Samples of normal size vacuum in every station e Mix of biopsies and normal samples vacuum in every station e Large samples cycles of pressure and vacuum in every station It is not recommended to simultaneously process sampl
17. filling from a reagent or a wax bottle has not occurred in the allotted time CAUSE See alarm 51 SOLUTION See alarm 51 EXPLANATION Before to fill the content of a tank the system tests the tank specific portion of pipeline If the pipeline is clogged the system tries to fill the following reagent and issues the alert 6 ABORTED STEP in case also the following bottle would be clogged the system will issue this blocking alarm and will stop the process CAUSE The pipeline of the bottle that the system is going to fill is clogged or the previous reagent was not completely drained due to the pipeline obstruction or due to some problem in the hydraulic or pneumatic circuit SOLUTION Check the specific portion of pipeline of the tank involved in this alarm or the previous tank there may be debris or drops of solidified wax that clogs it EXPLANATION During a wax step the processing chamber temperature went over the allowed limit not to be confused with alarm 20 CAUSE SPC thermoregulating system damage SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION Malfunction of the SPC temperature sensor CAUSE SPC temperature sensor failure SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION A reagent fill was completed before the minimum allotted time The SPC will be drained and the process will
18. is jumped and the process continues on the previous bottle which will be reloaded with the time of the jumped step If two adjacent bottles are found faulty a blocking alarm is issued and the process is aborted CSC cso Rev 120416 of 16 04 12 hs Page 34 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano E O Lar fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 Ww ww bio optica i At the end of a program the instrument maintains the last reagent in the processing chamber Press ENTER to empty the chamber After the reagent draining it is possible to open the SPC and take out the samples After the confirm that the samples have been taken out of the SPC the computer will prompt a message with a request to start the purge program If the program included paraffin steps the purge execution will be compulsory The purge is necessary to remove wax residue from the process chamber It is always necessary if wax was used during the last process It is advisable to perform any case a purge even if in the last process no wax has been loaded into the SPC only in case the loaded reagents where formalin or alcohols the purge can be jumped without any problem The FIRST purge program has been set and optimized by the manufacturer and cannot be modified but there are other 3 purge programs tha
19. more than once in a month it would be prudent to have the unit connected to its own power supply separate circuit and breaker To increase the instrument safety and reliability an uninterruptible power supplies UPS may be used to protect against power failures fluctuations and spikes EXPLANATION difficult during the reagent drain The instrument had to perform two times the drain CAUSE Insufficient draining speed probably due to partial clogging somewhere in the hydraulic circuit SOLUTION Verify the SPC draining holes verify the bottle contents the presence of solid residue can slow down the draining EXPLANATION the duration of the reagent drain was too short CAUSE Insufficient level of reagent in the indicated bottle SOLUTION Verify reagent levels EXPLANATION the instrument is not able to create the proper vacuum in the processing chamber at the scheduled time The processor will not try anymore to create vacuum in that step and will go on with the program During the next step if scheduled it will try again CAUSE not perfect sealing of the lid gasket SOLUTION check and clean the lid gasket EXPLANATION excessive level of reagent in the bottle during the fill the overfill is recognized and the filling is stopped before it can produce a failure The process can continue without problems CAUSE Excessive level of reagent in the bottle SOLUTION Verify reagent levels EXPLANATION it is impo
20. that there has been an interruption of power It may be due to a loss of power in the main line or merely due to the switching OFF of the instrument If the loss of power occurs during a process the VTP300 automatic power outage protection will allow it to resume the process program exactly from where it was interrupted The program resume will be performed according to the formula Steps 1 to 10 formalin alcohol xylene etc no special action the power failure time is considered processing time and the VTP300 continues as if nothing happened Steps 11 12 wax processing will pause for approximately as much time as the unit was without power waiting for the processing and the wax chambers to return to the proper temperature The power failure time and the related re heating time are NOT considered processing time CAUSES Loss of power in the lab a blown fuse or the simple switching OFF of the unit CSO Rev 120416 of 16 04 12 Page 43 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i SOLUTION If the power supply doesn t resume check the electric network plug and cord If necessary replace the instrument fuses If the instrument is found fully operational but this alert appears too frequently e g
21. the samples 4 Reset the instrument After the reset the instrument is again ready to run a program The alarm could simply have been an error in closing the chamber lid or something else easy to resolve Before to proceed please do what follow 1 Remove all samples 2 Check that the SPC does not contain any reagent residuals 3 Check reagent and wax bottle levels 4 Check that the bottles are correctly placed in their housing slots 5 Check that the waxes are melted and if possible verify their temperature 6 Check and clean the SPC lid gasket 7 Start a purge to verify the correct functioning of the instrument When the cause of the alarm is unknown or uncertain it could be helpful to check the voltage and the quality of the main power line If the alarm persists please call our technical assistance In the top left of the instrument rear panel there is a green o red button Its purpose is the complete reset of the instrument software program It must be used under the following circumstances When for any reason the user is unable to reset an alarm in the usual way When a user faces unexpected situations that cannot be resolved by any other means To reset the instrument s memory that holds variables pertaining to the state and contents of the SPC possible processes in progress possible actions in progress and or alarms in progress The procedure for using the reset button is the following Y Switch off the i
22. will appear showing the most important parameters and settings of the instrument SPC Sample Processing Chamber Pressure SPC Sample Processing Chamber Temperature WAX Wax chamber Temperature RMS RMS Reagent Management System status enabled or disabled RMS reagents Status of reagents maintenance OK RMS executed NEEDED RMS not executed or not completely executed OFF RMS disabled EWD Device not active on VTP300 model WCC Wax Cleaning System status ON active OFF not active UPS Uninterruptible Power Source status UPS battery Battery pack of the UPS charge Charcoal Filters 096 new 100 exhausted Process Total number of processes executed Status Instrument status STAND BY waiting for a process start Purge Cleaning cycle status DONE executed SPC content The number on the left indicates the reagent currently filled into the SPC the number on the right indicates the last reagent filled in the SPC Alarms the number of the current alarm t 44444 PELLE The MAIN menu day by day operations will appear by pressing the START button left side The SETUP menu will appear by pressing the SETUP button right side A synoptic showing the most important instrument components is always visible on the left side of the screen esa csQ E Rev 120416 of 16 04 12 Page 10 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faus
23. 0134 Milano T O na fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The information provided in this manual applies only to the instrument indicated on the front page of this manual The instrument is clearly identified by a label applied on the rear of its body Identification label sample io Optica Mod VTP300 tuens tit aphea ud BIO OPTICA MILANO S P A Ref VTP300 via Faustino 58 20134 Milano MI S N xxxxIlxxx Rating 100 240V 50 60Hz Power 1000W 20xx qu Histology Tissue Processor AN E Y SA Ve C MEIO EO LAI Uh amet gt 803400304 a Identification label symbols 2007 Year of manufacturing It indicates the various voltages at which the instrument can work by varying the connection in Rating the internal transformer another label in the proximity of the main power cable socket indicates the voltage at which the instrument is connected and at which it must work ia Manufacturer address C CE mark In Vitro Diagnostic Device Caution Please read accompanying documentation Do not throw on domestic garbage please follow the local rules for special waste recycling and treatment li Please read the instructions for the use CTUVus TUV Reinland of North America Conformity to United States and Canada safety prescriptions and standards
24. 1 e Fax Acquisti Export 39 02 21 54 155 Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 Rating 100 250V 50 60Hz not user adjustable Max power 1000 W Main Fuses 100V 12 5A 110V 10A 125V 10A 225V 5A 240V 5A 6 3x32mm T Weight 153 Kg empty Dimension in mm H 1300 W 720 D 600 Running ambient temperature 10 35 C Storing temperature 10 50 C Relative Humidity Max 80 not condensing 1EC1010 classification Protective Class 1 Pollution degrees 2 Overvoltage category Il 800V impulse 115V versions 1500V impulse 230V versions Max elevation 2500 above sea level Temperature controls precision 1 C Remote alarm socket and relay 48V DC AC 1A maximum Paraffin waxes Tanks Volume 4 reserve 2 5 L Paraffin waxes Melting Time Approx 8 hours Paraffin waxes Temperature Range 52 65 C default 60 Sample Processing capacity 300 standard cassettes SPC working temperature From ambient to 65 C Pressure range 400 1200 HPa Reagent Tanks Volume 10 2 5L Tanks for purge agents Volume 2 125L Printer Normal paper optional connected on serial port RMS Reagent Management System For all reagents purge agents charcoal filters WCC Wax Cleaning Cycle Efficient on site system no SPC filling Reagent Agitation Use
25. 13 Compatible rodga oreroraa a A E a OEE RES 13 Disposable pre filled bottles nn nnnnnn nn nn nnne nnn nn rn nnn nennen nnns 14 Filling reusable reagent Dotes cota iii 14 RFD Remote Fill and Drain System dai 15 Filling wax bottleS Saasen iaia aaa 16 Wax Cleaning Cycle WCC iii 18 PIOCESSING GApachy ciuialnli ms 19 SETUP OF the ins tument encontre nnne man aen RE I 19 v siu M 19 SAA 19 DateFormat gare TP a oia 19 BI CUENTE 19 L L Apo ELEM 19 6E ets 20 iiU tadas 20 SPG lid open Closed Serisor 5 lo eri tte edn leigh AL iii 20 IV 63 Mop HS 20 RED Calibration pe Hc 20 UPS unit Uninterruptible Power Source i 20 Panel On Time screen saver time it c AREE LER RUE Ro aiian aae aaa 20 MIR 21 TME and DATE iiie tere as A iaia Ce da aa ia 21 Password SOUP I RI RA AAT INTATTA RAR ti 21 Enable Disable PassWOF dernier E teet te dc iere at qux yo d Lio 21 Password modiflcatlon pieta poc ia i iii 21 Password protection map ans ttt he netta ii a LUDERE AF UR ARRA AR RR Ru NR E NATA ER E ax SE e DH REA E NN RR 21 REAGENT LABELS T 22 Nd 22 A AsEsidiec PH 22 Program
26. AA a mm EIU aas BIO OPTICA VTP300 HISTOLOGY VACUUM TISSUE PROCESSOR USER MANUAL CSQ Rev 120416 of 16 04 12 Page 1 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano is O P fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i General Warnings unico onc eter rhe reet dada adel den eee ee 6 Designated USE EET 6 Instrument type and instrument identification 02 0 eee eee eect eee ee nennen mne nnren enne nennen 7 Safety Precautions noniin lupa Ae asta en peed 8 Unpacking transporting and handling eene cnn rro cnn ancora ran 9 Bierzo To iii ai 9 Packaging and or preparation for transpott nennen enne nnne nennen nennen 9 Transportation sss RN 9 Introduction to the VTP300 Processor i 10 General features Erana EEE ENE ERNEK EN UE ERA EEEE EENEN CRA ENKE EA ADEE ENER EAEN ET ERRER KEE 10 User interface and keyboard nono nn nn nan 10 SAT A 11 Wl 11 Installation and Start Up cee ee cece cece eaae cece cece ee ceaaaeceeeeeeesceaeaeeeeeeeeeseaaacaeeeeeeesesecaeeeeeeeerseesecaeeeeees 12 Initial rz Te 1 no RR 12 Before starting a process 12 At the end of a process i 12 EEE I PTN PO I AEA ON P EE N T VEE E A A T A T E E A T 13 Reagent tanks ITAM eiii E EE AAO ian
27. Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i down to a level not sufficient to guarantee the wax melting the process continues till the last reagent If the problem persists before the draining of the last reagent the process is stopped see alarm 60 CAUSE The thermo regulation system of the wax chamber is out of order SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION the PNV Pressure Normalize Valve is the solenoid valve that provide to normalize the pressure inside the SPC making it the same of the ambient pressure if the pressure doesn t normalize within 3 minutes from the intervention of the PNV this alert is issued CAUSE Possible malfunction of PNV IOB wiring Possible clogging of pipeline between SPC and PNV SOLUTION Check and in case clean the small hole in the right wall of the SPC A small quantity of Xylene or xylene substitute can be introduced into the hole with a syringe if the clogging was due to a small drop of solidified wax after a few minutes the xylene may melt it try also to heat the SPC from the Service menu after 15 minutes or more try to execute the Pressure test from the service menu after the creation of the pressure press ENTER to release the it if the pressure normalizes the problem has been solved If the problem persists contact our serv
28. ESC three times in sequence The SPC will then be drained after that the samples can be removed As said the interruption of a program is only possible when the reagent is in stand by in the processing chamber and not during the other steps filling emptying positioning the rotating valve etc There are situations in which the processing chamber lid CANNOT be opened when the SPC is under vacuum Others in which it MUST NOT be opened during fill and drain operations NEVER open the SPC while a program is running in a phase other than Processing Alarms may occur and the computer control system may abort the process Moreover potentially toxic or flammable substances may exit from the SPC The SPC lid is equipped with a micro switch that detects if the lid is open or closed thus if the lid is opened during a process the process it is automatically suspended But before to open the SPC lid it is BETTER to suspend the process by pressing the keys ESC and ENTER in sequence This will signal the system to normalize the SPC pressure and equalize it with the ambient pressure As said the SPC lid can be opened when the message line displays Processing Please take the following precautions 1 Suspend the process before to open the SPC lid 2 Close back the SPC lid as soon as possible 3 Respect all the safety rules about precautions to be taken when handling toxic or flammable substances 4 If the system is processing during a vacu
29. MS The definition of the limits determines the frequency of reagent substitution It is possible to assign to each tank a limit based on the number of cassettes processed When this limit is reached or exceeded the RMS will require the substitution of the reagent for that specific tank If the limit is set to zero the RMS will never prompt the user to replace the reagent in that tank but its quality light will always be shown in red to remind that this tank is not managed by the RMS for a lack of limit It is advisable to fix the same limit to all the tanks of a group otherwise the RMS can ask for unbalanced substitutions with unpredictable results Moreover the limit can be proportioned to the length of a group in other words the longer is a group the higher can be the limit The Counters Total Reset selection allows the user to set to zero the number of processes performed and the number of cassettes processed including the DAF counter This function may be used when it is necessary to replace renew the reagents of all tanks and at all the effects restart the RMS The complete zero resetting does not include the counters for the charcoal filters or the tanks of the purge reagents These two counters can be reset using the RMS Single Counter Reset see next sub chapter Please note the counters total reset automatically occurs each time the RMS is activated or de activated When the definition of the reagents groups and or their lim
30. Rev 120416 of 16 04 12 Page 14 of 60 MED ISO 13485 The Remote Fill and Drain allows the draining and the filling of the reagent bottles with the exclusion of the waxes without removing them from their slots The RFD connectors are placed in the front of the instrument as shown in the picture in the previous page In the left side there is the one for the FILL while in the right there is the one for the DRAIN To those ports it is necessary to connect the hoses with quick connectors and 90 elbows that will be placed inside the external tanks with the waste and the clean reagent a jaja CS CS Instrument READY The RFD can work in three different modalities Modality DRAIN and FILL The VTP300 fills the SPC with the content of the chosen bottle Then it drains the SPC through port 17 into an empty tank right side quick connector Afterward it fills the SPC with 2 5 L of reagent from port 18 left side quick connector Then it drains the SPC content into the chosen bottle This modality completion takes approximately 6 minutes 999 Modality Only DRAIN The VTP300 fills the SPC with the content of the chosen bottle Then it drains the SPC through port 17 into an empty tank right side quick connector lesi MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Ass
31. See alert 30 SOLUTION See alert 30 EXPLANATION The password has been mistakenly typed more than three times The alert is stored in memory for future consultation CAUSE See alert 30 SOLUTION Type the correct password if the password is lost please call our technical service to retrieve it EXPLANATION The drivers of the touch screen device are not responding properly CAUSE Hardware failure of the touch screen devices SOLUTION If the problem persists contact our service department for technical assistance meanwhile it is possible to successfully and safely use the VTP300 by connecting an AT keyboard in the rear panel see sub chapter Rear panel All instrument functions can be controlled also by a common PC keyboard CS Rev 120416 of 16 04 12 Q Page 47 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i EXPLANATION The instrument was unable to produce vacuum in the process chamber while attempting to fill from a reagent or a wax station CAUSE During the filling stage a leak in the pneumatic or hydraulic circuit occurred the air pump is not fully efficient or broken SOLUTION Check the SPC lid gasket Please contact our service department for assistance EXPLANATION The
32. Xylene substitutes Paraffin Wax OOOoooo eso cso Rev 120416 of 16 04 12 hs Page 13 of 60 MED ISO 9001 ISO 13485 Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O a fica Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The following materials are used in the construction of the VTP300 Reagents other than those listed above can be utilized in the instrument if they DO NOT damage the materials listed The manufacturer is NOT responsible for damages due to the use of reagents NOT listed here WARNING DO NOT use Acetone Benzene Toluene or Trichloroethane We also advise against the use of fixatives containing mercury salts acetic or picric acid as they may corrode the metal components of the instrument and shorten a component s useful life The VTP300 can utilize disposable ready to use factory pre filled reagent bottles It is very important to note that the use of pre filled bottles is possible without making any changes to the instrument It can be done at the same time with the use of bottles refilled by the user the latter are simply thicker and stronger than the disposable bottles Advantages of using disposable pre filled bottles include a Faster reagent and wax replacement a Minimal user handling exposure to toxic vapours a Reduction of risks from handling flammable su
33. abling are related only to the VTP300 computer gt UPS unit communications Even if disabled by this function the UPS will continue to supply power to the VTP300 but its state will not anymore shown and considered In case of an attempt to enable the UPS when the UPS is not installed the command will be refused In case of serious malfunctions of the UPS it may be not sufficient the software disabling in this case it is advisable or necessary to physically disconnect it from the VTP300 Please call our technical service to perform the repair or the disconnection After a period of not utilization of the touch screen the LCD screen is completely switched off to extend its life span and save energy This function allows the setting of the panel ON time The minimum time is 0 minutes function disabled screen always ON The maximum time is 90 minutes The tissue processor will continue to work perfectly even when the screen is switched off by touching any point csQ Rev 120416 of 16 04 12 Page 20 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O pma fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i in the screen surface it will be switched on in not more than 2 seconds and the VTP300 computer will emit a beep to confirm It is advisable to not press more than on
34. after only a few minutes from the fill it may happen that the drain is not complete due to the formation of cold and solidified wax spots especially in the core of the SPC It may also happen that one of the cold solidified wax spots will clog the draining holes in the bottom of the SPC resulting in incomplete drain alarms This problem which is more or less common to any and each kind or brand of tissue processors is more probable with a full samples load with a low samples load for example 50 biopsies the problem doesn t practically exist any case it does not make any sense and it doesn t bring any benefit to the process to set step times shorter than 10 minutes A good timing choice for the first wax is a minimum of 1 minute every 10 samples thus 20 minutes for 200 samples and 30 minutes for 300 For the reasons described at point 5 the first wax is not subject to cycles of vacuum even when vacuum is set in the program that is due to the possible formation with high samples load of a sort of solidified foam on top of the sample baskets that will require long time for its melting The two phenomenons above described are more or less common to every kind and brand of tissue processor That is easily understandable by the fact that as said the first wax gets in contact with quite cold samples baskets and SPC walls The problem can be reduced if in the last reagent the temperature is set at 45 C In case of drain alarms in the first wax the mos
35. al pellets to get out 3 Transport the charcoal filter bottles separately from the instrument wrapped and securely closed in a protective nylon bag 4 f the original box has been saved follow the unpacking instructions in the reverse order using all the interior packaging to avoid serious damage to the instrument during shipping Before shipping please keep in mind that 1 The instrument is fragile 2 The instrument is equipped with electronic parts 3 Contact with water and or any other liquid is to be avoided please ensure that the internal plastic protection bag that wraps the instrument is utilized 4 Transporting and storing temperature s must be between 10 C 50 C 5 Using the original box the instrument is to be transported horizontally 6 Transporting the instrument vertically is highly discouraged as its centre of gravity is quite high eso cso Rev 120416 of 16 04 12 hs Page 9 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O ti ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The VTP300 tissue processor recycles the air utilized to move the reagents to and from the processing chamber Two effective charcoal filters on the external air intake reduce the exhaust fumes to acceptable and safe levels The wax and reagent bottles can be
36. an Faustino 58 e 20134 Milano 1 O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i In the example shown above none of the reagent requires maintenance The RMS table shows DAF Decreasing Aging Factor counter LIM predefined limits CAS counter of the actual number of cassettes processed PROC counter of the number of process performed SUBST the tank that needs to be changed The counters of the purge reagents and the charcoal filter The colour of the tanks indicates the quality of the reagents as follow GREEN new reagent or already used reagent but with DAF counter not exceeding its limit RED reagent that has reached exceeded the limit GREY tank with not defined content BLACK empty tank The screen will instruct the user to renew the reagent tanks that exceed the previously set limits By pressing the DO IT WITH RFP icon the current tank substitution will be made with the Remote Fill amp Drain system CAUTION gt To avoid mistakes it is advisable to faithfully follow the given instructions and to confirm by pressing CONFIRM each singular substitution gt The reagents must be substituted ONLY if the RMS requests for that gt Is it possible but not recommended to suspend the RMS operations by pressing ESC then later restart by pressing RMS from the START Menu and compl
37. and the text will report the word FAIL after a short while the UPS will cease to sustain the VTP300 and will automatically shut down When the main power is back the VTP300 and UPS will restart automatically but it will take at least one hour before the bar will return to be green If after a total of 3 4 hours the bar is still not completely green a battery failure may be present In this case it is necessary to call our technical service to repair the UPS or substitute the failed battery 2 As well as described at point 1 when the UPS battery charge is close to zero the UPS autonomously shuts csQ Rev 120416 of 16 04 12 Page 57 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O PF i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i off When the power is back the UPS and the VTP300 will automatically restart without the need to turn on any switch 3 The text FAIL in the UPS bar may be due to the lack of UPS batteries connection see previous chapter 4 For more information please consult also the UPS specific manual attached as external appendix to the present user manual Rev 120416 of 16 04 12 Page 58 of 60 MED ISO 9001 ISO 13485 10 Bio Optica Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano Tel 39 02 21 27 13
38. ar attention to the instrument setup instructions The warranty is invalid if the instrument is improperly operated Be certain to follow the instructions and recommendations provided by this manual The manufacturer is not responsible for damages resulting from improper operation or handling of the instrument Pay particular attention to the outside of the shipping container In the event of concealed damage save all shipping crates and packing material DO NOT unpack the instrument if damage is apparent Immediately notify the carrier of any damage and contact the shipper to initiate any claims The proper steps to be taken are 1 Open the top of the box 2 Liftthe side walls out of the way 3 Raise the instrument to the vertical position 4 Remove the plastic layers that wrap the instrument 5 Carefully check the external condition of the instrument In the event of evident damage DO NOT connect the instrument Immediately notify the carrier and promptly contact the seller 6 Forthe setup of the instrument see the specific chapter Installation and Start up in this manual 7 Save the box and all the internal packaging in the event that the unit requires future shipment To transport the instrument perform the following steps 1 Remove all reagents waxes included from their bottles 2 Remove and close with the proper threaded cap the charcoal filter bottles put also some adhesive tape on the air inlet to avoid charco
39. assword modification The password can be modified any moment from this function but of course the modification is allowed only after the correct typing of the current password We recommend to carefully keep note of the new password before the modification because once modified there is no way to deactivate or modify it without knowing the current one In case the password is lost it is necessary the intervention of our technical service to deactivate or retrieve it ES a El o 2 o 3 E o There are nine functions that can be protected by password The lab manager must decide what to protect in relationship to real security reasons It is advisable to use the password only where it is really necessary The extensive usage of the password while increasing the instrument security may create problem to the day by day operations The password will be requested for the activation of each protected functions and can be requested many times during the usage of the instrument for example during the RFD it will be requested for each bottle managed that may result to be bothering but it is necessary to guarantee a real protection of the instrument especially when the work done is multiple and complex as it is during the RFD If the RFD is under password its usage will be protected during an execution of it from the RMS even if the RMS is not protected CSC cso Rev 120416 of 16 04 12 hs Page 21 of 60 MED ISO 9001 ISO 13485
40. ation is higher than 800 1000 meter L L Alarming The displays of the ALERTs during the processing low level not blocking alarms can be enabled disabled CSO Rev 120416 of 16 04 12 Page 19 of 60 MED ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i The End Of Process beeping can here be enabled disabled If the printer is not installed this port must be disabled This way the program execution will be faster The SPC lid status is controlled by a sensor micro switch If the lid is closed but the system displays that it is open probably the micro switch is broken or out of position Waiting for service from this function the sensor can be disabled after that it is possible to continue to operate the instrument If the micro switch is disabled the SPC lid indicator will show the label DISABLE Please note that the sensor can reveal the lid complete opening but cannot sense its perfect closing Thus if the lid is simply turned down but the lock is not engaged the sensor may indicate lid closed but the instrument will not be available for processing The instrument will allow the process or purge start but soon it will issue an alarm caused by the impossibility to create vacuum or pressure in the SPC Please always ch
41. bstances a Eliminates the technician time required to empty clean and re fill bottles Replacement of disposable bottles is easy Take the bottle containing the exhausted reagent out of its housing slot a Screw the special cap on the opening of the bottle a Unscrew the cap of the new bottle a Insert the bottle into its housing slot O The procedures to be followed regarding the recycling of contaminated reagents are the same adopted with traditional systems VTP300 bottles are made of completely recyclable 100 HDPE high density polyethylene Check with your local recycler about recycling HDPE with chemicals and wax residue The emptying and subsequent refilling of reusable reagent bottles must be done in accordance with all safety regulations for handling flammable and toxic substances The procedure must be performed with proper ventilation and away from open flames and or electrical circuits Bottles must be filled to the indicated level a volume of 2 5 litres When exchanging bottles use the special screw caps supplied with the instrument to avoid reagent spillage and fumes After emptying dirty or encrusted bottles must be replaced with new ones Avoid cleaning them with solvents or similar products The o rings and all the openings on the quick couplers should be checked periodically for cleanliness If the quick couplers leak the o rings should be replaced If leakage problems persist contact our customer service CSQ
42. d to begin a process with the SPC either not empty or not cleaned from wax residue CAUSE The SPC is not empty The purge cycle compulsory after a process which involved waxes was not performed SOLUTION If the SPC is not empty drain it manually Enter the Service Menu and perform alarm reset and flag reset Return to the Main Menu and start the purge EXPLANATION An overfill of reagent or wax has occurred in the SPC Alarm sensed by overpressure during fill Normally an event of overfill is intercepted and solved by the anti overfill devices of the VTP300 in this case a simple alert is shown If the anti overfill devices fail to correct the problem this blocking alarm is called and the CS Rev 120416 of 16 04 12 Q Page 49 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O P fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it process is stopped CAUSE Complete emptying of the previous reagent failed due to an incorrect solenoid valve opening or in case of paraffin temperature problems SOLUTION No intervention by the user is possible If the problem persists contact our technical assistance 70 89 COM ERRORS EXPLANATION Problems with the serial lines of communication CAUSE Hardware failure SOLUTION Try to switch OFF and ON t
43. dling flammable reagents such as ethanol wear protective gloves and eyeglasses O Use specific precautions in handling liquid waxes as they can cause burns O The emptying and filling of reagent bottles must only be done only by qualified technicians O Due to the presence of flammable substances inside reagents bottles it is recommended to O Avoid smoking near the instrument O Avoid using open flames near the instrument e g Bunsen burner O DO NOT wear clothes that can create electrostatic charges while handling reagents wool synthetic fibres etc O Contaminated reagent waste must be disposed off in accordance with all applicable local laws ordinances and safety standards Use only original spare parts supplied by the manufacturer or by authorized dealers DO NOT extract bottles replace reagents or do other maintenance operations when the instrument is operating O To complete the knowledge on the instrument safety please read also the chapter SAFETY DEVICES near the end pages of this manual esa csQ Rev 120416 of 16 04 12 v hs Page 8 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it Before moving or transporting the instrument it is essential to carefully read this chapter paying particul
44. e RMS 1 The limit maximum number of processed cassettes is defined for each tank independently from the group to which that tank is assigned 2 The number of processed cassettes will be then stored in memory for each tank and will be reset after that tank reagent renewal 3 Also the number of processes performed by each tank is stored in the memory but it will be used only to be shown under request of the operator also this counter is reset after that tank reagent renewal 4 The real effective counter is a third one its name is DAF Decreasing Aging Factor The DAF is calculated from v The number of cassettes actually processed v The position of a tank in its group The concept is based on the assumption that in a group the reagent that will age more is the first one while the last is the one that will age less Thus inside a group the number of processed cassettes will be assigned to the DAF counters in a decreasing manner for example Group taken as example Clearing Processed cassettes per process 100 Regressive percentage of the DAF inside a homogeneous group 100 60 40 30 20 15 10 minimum admissible Predefined limit for the 3 tanks of this group 300 cassettes End of process 1 Tank Processed Cassettes DAF regressive counter 8 100 100 9 100 60 10 100 40 End of process 2 Tank Processed Cassettes DAF regressive counter 8 200 200 9 200 120 10 200 80
45. e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O P fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it There are various levels of protection that can be achieved with this system 1 Total protection all functions activated 2 Programming protection only the instrument programming functions are activated Edit Process RMS Setup Parameters Setup 3 Mid level protection recommended only the functions more delicate are protected Edit Process Abort Process RMS Setup Parameters Setup Service Of course also to access the password map modification it is necessary to know the current password The most common reagent names are already set in factory It is possible to modify them as desired Up to 40 labels are available here With this function various instrument tests can be performed Please see the chapter SERVICE This function is protected by a special factory set password and it is reserved to service technicians esa csQ E Rev 120416 of 16 04 12 Page 22 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it This function can be accessed from the START menu Sel
46. e instrument consists of Cleaning the processing chamber Cleaning the processing chamber lid and gasket Checking the level of reagent and wax Checking that reagent and wax bottles are correctly positioned in their slots The following monthly maintenance is recommended Check the indicator of the Charcoal filter and in case is at 10096 replace the filters Check for the presence of residues at the bottom of reagent and wax bottles When replacing reagents carefully clean their bottles Grease the processing chamber lid gasket with silicone or PTFE grease Grease the bottle insertion o rings with silicone or PTFE grease Filter replacement is required approximately every 60 to 120 processes based upon environmental factors and the use of the WCC Regardless of usage the charcoal filters should be changed at least once every six months Charcoal filters must be replaced with the required frequency as they may release toxic and contaminated vapours into the air when exhausted Exhausted filter waste is to be handled in accordance with the local regulations The processing chamber lid gasket is made of VITON rubber a DuPont trademark It needs to be periodically greased with TEFLON grease When replacing it use a plastic sharp tool not a metallic one to extract the gasket Before to insert a new gasket clean carefully the gasket housing slot The gasket replacement may be not easy due to the fact that to keep it in place its length i
47. e processing chamber Take samples out of the processing chamber Clean the SPC and lid of any wax residue Execute the purge program At the end of the purge program check that the SPC and lid are clean of any trace of wax or foreign bodies If necessary complete the cleaning manually Check the graph of the last process executed from the Main Menu press the key GPH to verify if the process has been executed properly and without any fault Ad 4l x AA FAL LIC Cid DODODDO gt O cso Rev 120416 of 16 04 12 Page 12 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O i CQ Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The following picture shows the arrangement of the instrument tanks for reagents waxes purge agents and charcoal filters as seen from the instrument front C2 Charcoal filter for waxes C1 Charcoal filter for reagents P2 Purge agent 2 Ethyl Alcohol 95 100 P1 Purge agent 1 Xylene or substitutes 11 gt 14 Waxes 1 gt 10 Reagents FILL Filling Port for RFD system DRAIN Draining Port for RFD system 28 BERE FILL oran The following reagents can be utilized in the VTP300 without any risk of damage Water Formalin Ethyl alcohol pure or denatured Methyl alcohol Xylene
48. e time the screen surface and wait for the screen data and pictures to be visible before to perform any command The first touch will not be considered a command but any subsequent touch may be recognized as a command Please see the RMS chapter ahead in the manual A screen opens to display day month year hour minute and second Press on the field to be changed then increase or decrease its values with and keys Press CONFIRM to save changes and to go back to the previous menu Press ESC to abort changes and to go back to the previous menu The VTP300 is endowed with a password system that allows the access restriction to the main functions of the instrument Password general rules In case of wrong password the system shows a specific error message The password field accept up to 12 characters or numbers mix is allowed Also empty spaces are admitted in the password composition Enable Disable Password This function allows the enabling and disabling of the Password If the password is not active the systems requires the definition of a new one If the password is active the system allows the disabling by typing the current password correctly We recommend to carefully keep note of the password before the activation because once activated there is no way to deactivate it without knowing it In case the password is lost it is necessary the intervention of our technical service to deactivate or retrieve it P
49. eck the correct lid closing and lock engagement before the start of any kind of operation This function allows the setting of the WWC temperature setpoint The range is 55 65 C It is advisable to set its value to the highest point compatible with the laboratory procedures Particularly it is necessary to set the value at least 2 C over the declared paraffin melting point For example for paraffin with a melting point of 56 58 C the correct WWC setpoint value would be 60 C This procedure allows the calibration of the volume of reagent filled during the RFD cycle Remote Fill Drain To find the right value proceed as follow Using the RFD execute a fill in one of the reagent bottles With a system sufficiently precise 10mL take a measurement of the filled reagent Set the calibration value considering that every increase of 10 corresponds to 25 ml Repeat the filling with the RFD to check if the correction has given the right result The standard precision of the RFD is 1 which corresponds respect to the standard value of 4 litres to a maximum of 4 04 litres and a minimum of 3 96 litres The above cited values are compatible with the correct functioning of the processor in normal conditions The VTP300 can be equipped with an optional UPS To be recognized by the VTP300 computer the UPS must be enabled by this function as well as it can be disabled in case of malfunctions Please note that both enabling and dis
50. ect a process protocol from the menu by pressing on the related icon The available protocols 18 A new screen will open showing the entire process content With the ARROW keys it will be possible to explore the entire table to select the field to edit Each field content can be varied by using the extended character keyboard or in same case by using the keys Each field can also be accessed by just pressing on it Press SAVE to save changes and exit the function Press ESC to abort the operation Below are explained limits and characteristics of each field Reagent names are labels prepared in advance as explained before They do not have an influence on the process The tank content is decided in the RMS setup menu with the function Define Reagents Therefore it is necessary to pre define them in advance even if the RMS is not used The station time can be set from 0 to 99 hours The data control function can give unexpected results in case a number higher than 59 is entered in the field of the minutes For example entering 1 or 2 digits and moving over another field the entered number is interpreted as minutes if the number is higher than 59 it is transformed in hours and remaining minutes thus 60 will be transformed in 1 hour and 0 minutes 65 will be transformed in 1 hour and 05 minutes and so on Entering more than 2 digits the result may appear more strange when the last two digits entered are higher than 59 Thus the number 159 will
51. ed it is necessary to switch the UPS OFF Moreover for total safety when it is necessary to work on internal parts after the UPS is switched OFF we also recommend to disconnect the UPS power cord from the VTP300 UPS installation and maintenance For the installation and the maintenance of the optional UPS please carefully read and follow the instructions contained on the UPS manual UPS behaviour in case of power failure Batteries status Action Nc Starting a process or a purge is not allowed independently from the batteries charge status Nc Independently from the batteries charge status when a process is automatically suspended due to a prolonged power failure it is not possible to manually restart it by pressing ENTER on the touch screen as it is possible to do with processes suspended by the user the process is kept in a suspended modality and the VTP300 is operated in power saving modality until the main power is back Nc To save energy the mixing and other minor actions are suspended since the very beginning of a power failure event 100 The SPC heating is disabled except when in the SPC are loaded waxes The lack of heating of the reagents for a limited time doesn t prevent a good quality processing 10096 The temperature of the WWC is temporary reduced by 3 degrees if the process is far from loading waxes this temporary reduction significantly increase the UPS autonomy 100 T
52. es of very different sizes The reagent mixing in the VTP300 is accomplished by bubbling air up from the bottom of the processing chamber Select from the following frequencies csQ Rev 120416 of 16 04 12 Page 23 of 60 MED ISO 9001 ISO 13485 t ww io Optica dio Optica I 0 no mixing 1 one mixing every 30 minutes 2 one mixing every 20 minutes 3 one mixing every 15 minutes 4 one mixing every 10 minutes 5 one mixing every 5 minutes Proc R ed Process 18 REVERSED possesses all the characteristics of the other programs except Q It begins at the last non zero time on the steps list and moves backwards REVERSED O Itis not possible to insert a delay on the first step a The operator cannot modify its name Q Its position in the list of the processes is always the 18 Except as specified above it is identical to the other processes therefore It increments the RMS counters All RMS rules are respected At the end of the process user will be prompted to run a purge cycle At the end of the purge cycle if during the last process at least one paraffin was used the WCC will be automatically started a Atthe end of the WCC the Reagent Management System will be started Oooo There are no particular restrictions to the use of this process however be aware that the reagent contamination will also be reversed 08 02 2012 16 14 48 io O plica
53. es with extension dat data files contained in the internal memory a All files contained in the internal memory For reasons related to the program files safety this two operations differ as follow o By simply activating this function the computer will copy only the files with the extension dat data files o By activating this function and keeping pressed the reset button located in the rear panel the computer will copy all files Be careful during these operations If the USB memory is not empty the space can be insufficient to backup all files Always use blank and formatted USB memories This function has the same effect of the Reset Button on the rear panel of the instrument This function may be used to eliminate an alarm appeared during the instrument testing It can also be used to reset an alarm that for any reason was not possible to reset by the normal way POS VR position TANK VR position required MinA2 MaxA2 Data reserved to service technicians Analog2 Range Centre P1 VR Micro Pressure SPC direct pressure in Hpa Hecto Pascal Timer Time necessary to make vacuum and pressure in the SPC Hold Elapsed time between two pump starts during vacuum pressure tests ALARM Current alarm code SPC cont SPC content after a fill it is set to the bottle number filled after a successful drain is set to zero SPC last Last reagent filled in the SPC bottle number
54. ess time gt Any update of the delay End time here made will be stored in the long term memory only if the program is actually started gt Obviously the instrument needs sufficient time to complete the selected process If it isn t possible to complete a process at the desired time the computer will propose a different End time Any case it is highly recommended that the user verify time day and date of process completion before starting a program If the End time is incorrect after the start of a program the only user option is to abort the process and restart it again with a corrected End time Process programs cannot be edited during processing Start from step different from the first At the start of a program press the UP and DOWN arrows note that the bar that highlights the step will move from the first step to every valid step every step is valid if its time value is greater than 0 This feature can be useful for example on partially processed tissue samples to re start their processing where it was interrupted After the process End time and the number of cassettes and baskets has been set the data can be stored in the memory by pressing SAVE then the program can be started by pressing START Pressing the ESC key the operation can be aborted After the final start the following information will appear on the screen a Each step of the process with time temperature pressure and mix data The current step hi
55. ete it Cso Rev 120416 of 16 04 12 Page 31 of 60 MED ISO 9001 ISO 13485 p T Ms p S Vaart y E SS Processing with the VTP300 Check paraffin and reagent levels Check and if necessary clean the processing chamber Place the samples into the processing chamber Close the processing chamber lid firmly Select a program Edit date and time of process end SNAKE Before to start a process program it is necessary to check and in case to set the program time and date of completion dio Optical y 08 02 2012 l Thursday 09 02 2012 7 Cassette Nr 300 y EOP Time 07 gt 72 02 ALCOHOL 95 OE EOP Day Thursday K AL OL 95 01 00 0 Delay 00 days 01hours 46min E o 9 Instrument READY TA CSQ iei MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i In the above figure the top left window contains all the EOP parameters The indication of the number of cassettes is compulsory Please Note remaining in this screen for every minute that elapses the delay if any will decrease by one minute while the Program End time remains constant But if the delay time is set to or reaches zero the Program End
56. f malfunctions the relative alarm is shown at screen but it is not possible to have a full report with the values of the most important parameters for example the time required to reach the full vacuum or pressure This test allows to e Check the SPC heating elements e Melt the wax residuals that could remain in the SPC in case of an alarm e The processing chamber temperature is automatically brought to 60 C This process may require up to 30 minutes This function displays the last 100 alert alarm From left to right are shown date time alarm step in which the alarm was issued Only 18 alarms at time are shown starting from the more recent With the key DOWN it is possible to show the remaining Rev 120416 of 16 04 12 CEQ hs Page 51 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O P fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it With this function it is possible to save on a USB memory all instrument data files later in case of need the saved files can be restored into the instrument memory The restore operation can be performed only by the service technicians Before to start this function it is necessary to insert in the USB port a blank formatted memory From this function it is possible to either copy into the USB memory a Only the fil
57. fferent utilization of the reagents The reagent management is made more complex by the fact that while slow overnight processes use all the reagents fast processes use only a few reagents normally one per group Moreover in these fast short processes there is the need to use the best reagent for each group The concept of homogeneous group is based on the type of reagent and the kind of work that it does In the tissue processing 4 fundamental types of reagent can be identified X e Fixative Dehydrant Clearing agent Embedding agent X eS X k e e The dehydrants can be split in two sub groups low and high gradation Sometimes a tank with water is set between fixative and dehydrant to remove from the samples as more as possible fixative that in contact with alcohol can form noxious salts Thus considering that a group can be constituted by one tank usually in a tissue processor there are 4 to 6 groups Some users set the dehydrant in a way to form an incremental scale that can bring the number of groups over the above mentioned total of 6 lt is here important to note that the VTP300 RMS accepts a maximum of 8 groups It is highly advisable to not form too many groups 5 or 6 groups are the ideal situation for a successful reagent management and the samples best quality Particularly a maximum of two groups of dehydrants are sufficient to guarantee a correct incremental alcohol gradation But also a single big gr
58. ghlighted In the highlighted step the time remaining will ramp down minute by minute until the step is completed On the top of the process window the name of the current program and the scheduled date and time of completion and other information are shown Once a program is started it is not required any other user intervention Processing step times and total time include the time necessary to fill and empty the SPC Ooo CS Rev 120416 of 16 04 12 Q Page 33 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O Lar i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i When a process program is running it is possible to suspend and later abort it but only during stationary phases for example not during filling and draining To suspend a process press SUSPENSION and within 3 seconds ENTER The instrument will suspend for 3 minutes every activity During this period it is possible to open the SPC lid Pressing the key ENTER the process resumes If the process is not resumed within 3 minutes the instrument will try to do it by itself If the SPC lid is not closed an ALERT will be issued and a beeper will alert the user of the mistaken situation When the process is suspended it is possible to abort it by pressing the key ESC and after 1 second pressing again
59. he Main Menu or from the Service Menu if none of these menus is visible it is necessary to switch the instrument OFF after 10 seconds switch it ON again keeping the reset button pressed until the Main Menu appears This function is used to empty the SPC in case for any reason for example a blocking alarm during a process reagent residues are still present into it Before to use this function it is important to remove the causes that create the alarm for example by substituting the SPC lid gasket if the alarm was vacuum pressure related eso cso Rev 120416 of 16 04 12 hs Page 50 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O P fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it Activating this function firstly the processor checks the status of the rotary valve and the SPC last and SCP cont flags In case of inconsistency between the flags and the rotary valve position or in case the position of the rotary valve is unknown POS value to 99 the processor will require the operator to decide in which bottle drain the SPC content In this case it is solely responsibility of the operator to choose the right bottle an empty bottle or a bottle with a residue volume sufficient to contain the SPC content Pressing ENTER the processor will perform a normal drain pr
60. he creation of pressure and vacuum in the SPC is disabled the temporary lack of pressure or vacuum is not vital for a good quality processing but the UPS autonomy is significantly increased 4096 The process is completely suspended until the power is back No fill drain actions are performed in case the suspension happens during a fill drain the actions are completed to ensure that the samples will not dry in air The processing timer continues to work when the power is back if the step time is expired the following one is loaded Nc batteries charge status not considered in this action Please note as explained in other chapters to be recognized by the VTP300 computer the UPS must be enabled in the INSTRUMENT SETUP function In case of malfunctions of the communication system the UPS can be disabled The communication will be suspended but the UPS will continue to backup the VTP300 Attempts to enable the UPS when the UPS is not implemented will result unsuccessful In case of malfunctions of the UPS electrical power system its disabling from the Instrument SETUP may not be sufficient to allow the VTP300 a correct functioning in this case it will be necessary to call our technical service to either repair the UPS or physically disconnect it from the VTP300 Important notes 1 During a power failure if the UPS is used up to the complete batteries discharging less than 20 of residual charge all the bar squares would be red
61. he instrument If the problem persists contact our technical assistance 90 93 I O ALARMS EXPLANATION Problems with the I O systems CAUSE Hardware failure SOLUTION Try to switch OFF and ON the instrument If the problem persists contact our technical assistance The alarms historical archive is under the SERVICE menu and consists of a list of the last 100 alarm occurrences Thanks to this feature a service technician can immediately recognize the problem its frequency the step in which it occurred and other information useful to solve the problem Alarms are displayed from top to bottom on the screen starting with the most recent one Use DOWN arrow to display older alarms Use ESC to go back to SERVICE menu If a printer is available the alarm list can be printed from the SETUP menu With the service functions the following tests can be performed This function is used to calibrate the TOUCH SCREEN position in respect to the LCD screen After the activation of this function it is necessary to carefully follow the procedure indicated in the screen At the end of the calibration the system will automatically restart CAUTION in case of errors during the calibration the Touch Screen may not be working properly in this case it is possible to reset the calibration to his previous values by pressing the reset button placed in the back of the instrument for 12 seconds The reset operation must be executed from t
62. he one of the last executed process The graph visualizes for each step what follow O The temperature of the SPC a The temperature of the WWC a The pressure of the SPC a Any occurred alarm or alert a The program steps There are two windows a The upper one is reserved to the temperatures and the alarms a The lower one is reserved to the SPC pressure In the event of an alert alarm their visualization is made by a small box with the alert alarm number inside it an arrow that points into the graph indicated the exact time in which the alarm occurred All of it together with the alarm archive in the service menu may be of great help on determining what really happened in case of a faulty event during the process The graphs other than to be helpful in case of an alarm may also allow the avoidance of a future faulty event For example a not sufficiently stable temperature or pressure graph may indicate a degradation of the temperature control system or the air pump performances In this case it will be possible to call our technical service for a preventive maintenance and avoid possible future alarms and the risks of not complete processes Please see in the following pages some graph examples CSO CS E Rev 120416 of 16 04 12 Q Page 40 of 60 MED ISO 9001 ISO 13485 Dio Optica www bio optica it Example 1 process successfully executed 08 02 2012 io Optical WWC
63. heater safety thermostat SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION At the moment of the fill of a wax step the temperature of the WWC chamber was too low The process will be halted because the fill cannot be guaranteed CAUSE Incorrect working of WWC temperature sensor heater safety thermostat SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION The temperature of the wax chamber is well above the set point CAUSE WWC temperature system failure SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION The WWC temperature sensor is out of the correct range CAUSE WWC temperature sensor failure SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION During the ending phases of the drain the pressure in the SPC is too low CAUSE The air pump or other pneumatic circuit parts failed SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION During the drain the pressure has not reached the correct value CAUSE see alarm 63 SOLUTION see alarm 63 EXPLANATION The drain has gone over the maximum time allowed CAUSE see alarm 63 SOLUTION see alarm 63 EXPLANATION The user attempte
64. ice department for technical assistance EXPLANATION M1 is the name of the motor that provides the clockwise rotation of the VR Rotary Valve If the position of the VR doesn t change within 10 seconds from the starting of the clockwise command this alert is issued This is not a blocking event because the instrument may continue to work by using the counter clockwise motor M2 CAUSE Possible malfunction of M1 wiring IOB SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION M2 is the name of the motor that provides the counter clockwise rotation of the VR Rotary Valve If the position of the VR doesn t change within 10 seconds from the starting of the counter clockwise command this alert is issued This is not a blocking event because the instrument may continue to work by using the clockwise motor M1 CAUSE Possible malfunction of M2 wiring IOB SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION The micro switch of the VR is not working properly It is not sufficient to stop the operation of the instrument The VR continues to work but one of the control functions of the VR is not available CAUSE Possible malfunction of VR micro switch wiring SOLUTION No intervention by the user is possible If the problem persists contact our service department for technical assistance mean
65. ill be re started In case of events that would have stopped half way the execution of the VTP300 for example alarms or mistakes on the tanks positioning if some reagent is left into the SPC it is possible to remove it draining it back to a bottle by using the function DRAIN SPC in the Service menu It is highly advisable to take every precaution during the handling of reagents potentially flammable or toxic Despite the RFD operator safety increases thanks to the fill drain automation there is always some risk on handling flammable and or toxic substances We recommend to always have at hand a suitable extinguisher or at least to know the exact location of the closest one accordingly to the local rules of your country and laboratory CAUTION always check what follow Verify that the external pipes are well connected the quick couplers and are correctly inserted into the external tanks Verify that the external tank for the waste reagent would have at least 5 litres remaining capacity Verify that the tank with the new reagent would have at least 2 5 litres available for the filling and that the pipe end reaches the tank bottom Verify that the room in which the RFD is performed would be adequately ventilate the RFD doesn t have a recirculation system on the external tanks In case different kinds of reagents are drained together into the external waste tank before to do it in order to avoid potentially dangerous chemica
66. iners The volatile reagents evaporate due to the heating of the wax containers during the WCC process The air bubbled through the wax carries these reagent vapours through the charcoal filter C1 where them are trapped Use of the Wax Purification Cycle while it will reduce consumption of waxes will also shorten the estimated life of the charcoal filters therefore we estimate that e Without the use of the WCC filter life will be from 90 120 processes e With the use of the WCC filter life will be from 60 90 processes These values are only estimates because they are influenced by Environmental factors humidity temperature The duration of the processes The application of the vacuum or pressure on all the steps of the process The frequency of reagent agitation The duration of the Wax Purification Cycle is about 30 minutes If the WCC option is selected it will begin automatically after each purge cycle but only when a wax has been used in the preceding process It is always possible to manually run a Wax Purification Cycle from the RMS Setup Menu independent of wax usage in the previous process During the WCC the waxes are not transferred from their containers the SPC will stay clean and it will not be necessary to start a purge cycle after the WCC However during the WCC the SPC lid must always stay closed Ser CS Rev 120416 of 16 04 12 Q Page 18 of 60 MED ISO 9001 ISO 13485 e Bio Optica Mi
67. information that may allow to better understanding why an alarm was issued See also the preceding chapters for more information on the graphs The alarms numbers are shown inside a small box and an arrow points to the graph to indicate the exact moment of the happening Checking also the alarm archive the chances to understand why an alarm occurred are greatly increased For example Q If the wax temperature graph is unstable more than 1 0 C or if the temperature shown is different from the pre defined setpoint it is possible to suspect a failure of the wax temperature control system These temperature problems may also cause drain or fill alarms a Analogous situation may happen with the SPC temperature a The pressure graph allows the control of the air pump efficiency and the good sealing of the pneumatic circuitry In case of leaks the graph will be not stable to indicate an excessive frequency of the air pump start to restore the requested pressure into the SPC a A blocking alarm may be foregone by one or more alerts in the processing screen it is shown only the last alert alarm occurred in each step in the graph all the alerts alarms are shown even when happened in the same step That can be useful to understand what really caused the final blocking alarm a From the graph it is also very easy to verify if one or more steps has been jumped and the reason why it happened 1 PW FAILURE EXPLANATION the alert 1 indicates
68. ion of the batteries and the correct functioning of the UPS itself It acts as a filter to eliminate noises that can come from the electrical power line It will issue an alert and a resounding warning in case of power failure Usually a small UPS doesn t allow the tissue processor to terminate a process in case of prolonged power failure but considering that most of the power failures are shorter than 15 minutes it reduces the possibility that a power failure will create a failure on the tissue processor functioning The VTP300 is endowed with a system to make it capable to overcome power interruptions but that system cannot guarantee 10096 success The system consists in a special memory capable to retain its content also when the computer doesn t receive electrical power in that memory every action of the tissue processor is stored every minute or less when the power is restored the VTP300 will re start the process exactly from where it was interrupted Unfortunately some of the power failures are also characterized by sudden oscillations of the voltage that can overcome the VTP300 electrical protection systems furthermore some of these events may be characterized by a timing fast transient particularly harmful for the computer s memory It is impossible to foresee how many times a power failure may disrupt the functioning of the VTP300 it depends also on the quality of the electrical power supply a fast transient or spike or a very shor
69. istenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i This modality completion takes approximately 3 minutes it can be useful in case there is the need of a complete washing of the chosen bottle before to proceed to its filling Modality Only FILL The VTP300 fills the SPC with 2 5 L of reagent from port 18 left side quick connector Then it drains the SPC content into the chosen bottle This modality completion takes approximately 3 minutes Caution obviously in this last modality the chosen bottle must be empty before the start of the operation in case the fill is performed on a not empty bottle there will be a spill of reagents into the nearby bottles and possibly also outside of the instrument After the first few steps in which the computer asks for information regarding the bottle required for fill drain and confirmation about the presence of tanks connected to the ports 17 and 18 all the cycles are completely automated and the instrument can be left unattended until the operation ends See also sub chapter SETUP gt PARAM SETUP gt RFD calibration for more information about the RFD precision and its calibration The RFD can also be used in conjunction with the RMS during the reagents substitution It is possible but not recommendable to interrupt the execution of the RFD by pressing the ESC key Pressing again the ESC key the RFD will be definitely aborted while pressing ENTER it w
70. its are modified the system doesn t make any automatic reset of the counters That was decided to give to the user the maximum freedom but in case of modifications on the run it will be necessary to act carefully and eventually manually reset all or some of the counters see next sub chapter The Single Counter Reset selection allows the user to reset the counters of individual tanks This function is useful if the user decides to replace renew a reagent before the preset limit of a particular tank is reached The Single Counter Reset is also the only function that allows the operator to reset the counters of the charcoal filters and the purge reagents Please note The counters of the charcoal filters and purge reagents are not reset by the Counters Total Reset function Whenever the user replaces the purge reagents or the charcoal filters without being prompted by the RMS it will be necessary to utilize the Single Counter Reset to reset their counters This function turns the RMS ON or OFF Every time the RMS is set ON or OFF all reagents counters are set to zero In this case it is recommendable that all reagents will be replaced prior to use the instrument again csQ Rev 120416 of 16 04 12 Page 28 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite
71. jump from the ambient temperature to the 60 C of the waxes Moreover pre heating the samples and the SPC and the baskets helps to reduce the unavoidable cooling of the first wax that always happens especially with high loads of samples this phenomenon must be avoided especially when it is requested a short step time for the first wax because an excessive cooling may impede a good drain of it One or two mixing for each step is normally sufficient to ensure a good reagent movement around the samples More mixing are advisable for high sample loads At this regard it is necessary to consider that the SPC floor continuously receives a moderate heating from the WWC ceiling this heating creates a convection movement that results in a gentle but forced and homogeneous mixing of the reagents Furthermore that convection mixing is increased when the SPC heating is set ON eso CSQ Rev 120416 of 16 04 12 es Page 36 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O Lar fi a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Step Reagent Time TMP PV MX minutes 1 Formalin A A 2 2 Dehyol 95 60 A V 2 3 Dehyol 95 60 A V 2 4 Dehyol 95 60 A V 2 5 Dehyol 100 60 A V 2 6 Dehyol 100 60 A V 2 7 Dehyol 100 60 A V 2 8 Xylene or Bio Clear 60 30 V 2 9 Xylene or Bio Clear 60 40 V 2 10 Xyle
72. l reaction verify their chemical and physical compatibility In the VTP300 the paraffin waxes are contained in the same bottles used for reagents The advantages of it are the following a Easy handling and emptying of the wax bottles a Easy and economical substitution of the wax bottles a Possibility to use the instrument in Factory pre filled modality also for the waxes csQ Rev 120416 of 16 04 12 Page 16 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O fi C a Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilita 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it There is only one disadvantage bottles must be re filled with melted wax Attempting to fill them with flakes other than to be uneasy may produce under fill conditions that will cause a blocking alarm Furthermore the filling hose cannot be inserted into bottles 11 14 located in the wax heating chamber in presence of not melted wax The filling level is the same for reagents and wax 2 5 litres Wax bottles are placed in a special heating chamber that maintains a constant temperature consistent with the temperature required for the waxes during the process There are 5 wax bottle slots The one labelled R contains a spare bottle thus In Factory pre filled mode the R slot in the wax heating chamber is used to melt the paraffi
73. lano S p A e via San Faustino 58 e 1 20134 Milano E O i CQ Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The VTP300 is equipped with 2 stainless steel baskets of identical dimensions Each of the baskets can hold up to 150 cassettes The total processing capacity therefore is 300 cassettes As optional accessory is available a large basket that can hold big specimens or up to 350 STD cassettes As with all tissue processors good processing quality requires that certain guidelines be followed e Processing every day large quantity of samples the reagents will need to be replaced more often e Larger specimens require longer times at each station e The use of vacuum or pressure vacuum cycles and reagent heating in each step of the process improves infiltration e The daily replacement of the most contaminated reagent with subsequent shifting of the remaining reagents is preferable to the periodical substitution of all reagents In this way the process quality remains constant while in the latter method process quality varies from a maximum with all new reagents to a minimum last process before all reagents are replaced The VTP300 Reagent management System will minimize the shifting process work because it will not be necessary to physically move shift the reagent bottles into adjacent slots The RMS will automaticall
74. ming process protocols rice rre pce e a aiar 23 pta 23 uluyzme PH 23 CSQ Rev 120416 of 16 04 12 Page 2 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O a fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilita 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Temperatures Tom eR 23 Processing pressure Piscina aint 23 Reagent agtaton MA MM 23 Process 18 Reversed eee Dee a 24 Reagent Management System RMS iio iii e e ERR YE ML Rd TELA 25 RMS basic cConcepts RES 25 The DAP Decreasing Aging Factor aaa aa 26 RMS SUP iia S 27 RMS Detine Reagerits iuaceren ilaele 28 BSUaPIiDAM Incem 28 RMS Counters Total Reset urere ere eene ekle rear a e Ere eer A iaa 28 RMS Single Counter Reset iris indiana 28 RMS On Off cerca Remp eee eee erai parere a 28 Other functions of the RMS setup Menu 29 Purge reagents limits Setting aaa 29 Charcoal filters limits Settings sevice oerte esi is 29 WCC activation desactiva acia addict 29 Manual start of the WCC 0E 30 RMS PIMS aeri sua Rai alain 30 Using the RMS iia idad 30 Processing with the VTP300 cancion inet nter adc pcc t e p eda Lcd rec d ep Maccab cul tcr eld 32 Checks and
75. n prior its utilization as a replacement of a dirty bottle In Standard mode the R slot can be used to keep a wax bottle warm for immediate replacement of a dirty bottle CAUTION Since the normal wax temperature approaches the level at which a first degree burn may be possible we recommend the following Always wear protective gloves and eyewear when handling bottles of wax Always place a cap on wax bottles immediately after their removal from the wax heating chamber Handle them with care The wax heating chamber door is insulated and must be kept closed except when extracting or replacing bottles WARNING Never leave a slot without a bottle the bottles should always stay in their slots with their normal wax level of 2 5 It The absence of one bottle may cause an incorrect heating of the other bottles View of the wax warm chamber WWC esa CSO Rev 120416 of 16 04 12 has Page 17 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano E O Lar fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Use of the WCC will reduce the consumption of paraffin by approximately 5096 The system is able to reduce paraffin consumption by removing the contamination of the preceding reagents The removal is accomplished by a flow of air bubbled through the wax conta
76. ne or Bio Clear 60 45 V 2 11 Wax 58 60 60 A 2 12 Wax 58 60 60 V 2 13 Wax 58 60 60 V 2 14 Wax 58 60 60 V 2 Total Time hours minutes 14 00 Step Reagent Time TMP PV MX minutes 1 Formalin A A 0 2 Dehyol 95 20 A V 0 3 Dehyol 95 20 A V 0 4 Dehyol 95 20 A V 0 5 Dehyol 100 20 A V 0 6 Dehyol 100 20 A V 0 7 Dehyol 100 20 A V 0 8 Xylene or Bio Clear 20 30 V 0 9 Xylene or Bio Clear 20 40 V 0 10 Xylene or Bio Clear 20 45 V 0 11 Wax 58 20 60 A 0 12 Wax 58 20 60 V 0 13 Wax 58 20 60 V 0 14 Wax 58 20 60 V 0 Total Time hours minutes 4 20 CSQ Rev 120416 of 16 04 12 Page 37 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Step Reagent Time TMP PV MX minutes 1 Formalin 60 35 A 3 2 Dehyol 95 90 35 V 3 3 Dehyol 95 60 35 VIP 3 4 Dehyol 95 60 35 VIP 3 5 Dehyol 100 90 35 V 3 6 Dehyol 100 60 35 VIP 3 7 Dehyol 100 60 35 VIP 3 8 Xylene or Bio Clear 90 35 V 3 9 Xylene or Bio Clear 60 40 VIP 3 10 Xylene or Bio Clear 60 45 VIP 3 11 Wax 58 90 60 A 3 12 Wax 58 60 60 V 3 13 Wax 58 60 60 VIP 3 14 Wax 58 60 60 VIP 3 Total Time hours minutes 16 00 csQ Rev 120416 of 16 04 12 Page 38 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano 1 O Lar fi ca Tel 39 02 21
77. nstrument the switch is located near the main power cord eso cso Rev 120416 of 16 04 12 hs Page 42 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano 1 O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Y Press and Hold the reset button v Switch on the instrument and maintain the reset button pressed until the Main Menu screen appears After this operation the VTP300 may be ready for operations again but before to start any kind of process it is necessary to verify That the SPC is empty That the SPC is clean above all in reference to the presence of paraffin residue That the lid gasket of the SPC is clean and in its correct position That the instrument works correctly by performing tests from the service menu That an alarm present before the reset is not again on the display in this case will be necessary to switch off the instrument and call our service department with the exception of the alert 1 Before to start the purge cycle execute the instrument check list Run a purge cycle to verify that the instrument is fully operative VVVVV VV In the next paragraphs each alert alarm and the appropriate solution is described In case of an alarm it can be useful to consult the graphs of the last 30 processes executed to verify if there is
78. ocedure in case of blocking alarms the procedure will be stopped Caution do not use this function in case of not melted wax would be present in the SPC In this case it is necessary to first melt the wax with the service function SPC HEATING When the wax is completely melted the drain procedure can be started but being certain to choose a rotary valve position from 11 to 14 For safety reasons if case of presence of reagent the SPC lid must remain closed all time The instrument will create vacuum in the SPC this test must be done with the SPC empty and clean The SPC must reach a pressure of 600 HPa see Pressure field within 35 seconds see Timer field If the time exceeds 35 seconds the SPC lid gasket must be checked and cleaned If this action does not correct the problem contact our service department for technical service The instrument will create pressure in the SPC this test must be done with the SPC empty and clean The SPC must reach a pressure of 1200 HPa see Pressure field in a maximum time of 15 seconds see Timer field If the time exceed 15 seconds the SPC lid gasket must be checked and cleaned If this action does not correct the problem contact our service department for technical service This function allows the execution of the instrument full testing it is better to execute it with a paper printer connected in this way a full report is printed Without the printer the test is still useful because in case o
79. ocess would be surely influenced in the real world by variations in the quantity of cassettes processed and by the usage of fast short processes with only one tank per group In this case without using the RMS it would be quite complex to keep trace of the reagents unbalanced aging At the opposite thanks to the RMS the tracing of the reagents aging is automatic precise and reliable The RMS is not as rigid as it may appear if for any reason malfunction of a tissue processor component accidental loss of reagent from a tank etc it is necessary to substitute a reagent independently from the RMS requests that can be done by the RFD that will automatically reset the tank counters or manually in the second case it will be necessary to reset the tank counters by the RMS setup menu Of course during the next process that tank will be the last used in its group The VTP300 RMS is a system to punctually and exactly manage all reagents to guarantee a constant process and sample quality The use of the Reagent Management System eliminates the need to make annoying written annotations of reagent tank status This results in substantial time savings and eliminates the possibility of errors caused by multiple operators using the instrument csQ MED ISO 13485 Rev 120416 of 16 04 12 Page 27 of 60 ISO 9001 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export
80. of the manual There are no electrical signals on the remote alarm contacts They are isolated from the rest of the instrument and can be used to activate an external device such as an Auto Dialler to call a pre selected phone number and relay the message that an alarm occurred Rating of the remote alarm connector and its associated electronics Maximum voltage 48V ac dc Maximum current 1A Before the start of a main function process or purge the instrument executes a series of test to verify if there are the correct conditions to safely and successfully operate If the test fails the requested action is aborted and an alarm is issued The tests are referred to a The temperatures of all the heated components those temperatures must be compatible with their correct operational ranges a The SPC lid closing if the lid is open an alarm is issued Q The correctness of the signals from the pressure transducer a The correctness of the signals from the VR position sensor a The content of the starting process program if the program is incomplete or part of the data is lost or inconsistent the process cannot be started Q Various memory variables to make sure that a process is not started with the SPC not empty or dirty o Active alarms no active alarm can be present at the starting of a process or purge a The UPS if a UPS is present and active and there is a power failure even if the instrument is working thanks to
81. on against over heatingsS ii 53 eso e Rev 120416 of 16 04 12 Page 4 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano 1 O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Protection against OVer pressUres cr petet bi b da eed ed ed dE Pe edid 53 Fuse replacementi ELEM 53 NN 54 UII TU 54 SPC lid AM TR 54 Instrument MAINTEMANGCE oi A A ce NEL eee AA a 55 Dailyimaintenance ii iaia iaia ia 55 Periodicalimaintenance i unitarie 55 Charcoaltfilters replacement riattare 55 SPC lid gasket replacement iaia 55 ACCESSO ES inicia e A a 56 A AN 56 UPS Uninterruptible Power Source ssssssssssssssesesseee eene entree nr 56 General description dier eee ce A EL dd Ere ee e ta 56 Switching the UPS ori and off 1 ciet aaa LER Codd eee Lade nae 56 UPS installation and maintenance i 57 UPS behaviour in case of power failure 57 Important Notesniki eann a aa a a ea 57 Techhical s pecifications EE 59 Units of measure and abbreviations i 60 Pelei 60 iSo Ie cem EE 60 itle M 60 Cso Rev 120416 of 16 04 12 Page 5 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1
82. oup of dehydrant has demonstrated to work fine provided that at the first start of the instrument a certain incremental gradation has been manually created Other important concepts of the VTP300 RMS are The reagents are maintained at the end of every process to keep constant the specimens quality The VTP300 RMS most important concept is based on the assumption that the constancy of the quality is more important than the quality itself The reagents used first in each group are those that are more polluted by the previous reagent and by the substances removed from the samples Accordingly those are the reagents that require the most frequent replacement During the reagent maintenance the RMS will require the substitution of only one reagent per group also when more than one tank in that group has exceeded the pre defined limit In a fast short process with only one reagent per group activated in the process program the RMS will select and use the tank with the best youngest reagent independently from its position inside a group meaning for position both the real position and the programmed step position of that tank Rev 120416 of 16 04 12 CSQ cse hs Page 25 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio op
83. pen pins 1 3 closed This socket is a low voltage connection maximum 48V 1A insulated from the rest of the instrument An Auto Dialler or other external alarm notification devices can be connected here Reset button keep it pressed during instrument boot to reset the instrument transitory memory Power Supply Switch it must be always at ON PEZZI E Rev 120416 of 16 04 12 Q Page 11 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano 1 O pma fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i After the unpacking and the electrical connection to the main line the instrument is almost ready for the use there isn t any particular transport internal device that needs to be removed The next steps will be A Installation of the two charcoal filter on their slots C1 and C2 Remove the cap from the charcoal filter bottle and make a hole with a screwdriver or a pencil in the tape that seals the air intake on the top rear of the bottle B Connect the LCD screen The LCD screen is transported unconnected in a separate cardboard box The screen must be placed on the top left of the instrument housing The electrical connection is made by connecting the 2 connectors on the instrument rear panel One must be connected to the VGA the other in one of the USB C Connect the instrumen
84. procedures before operating sss eren n nene terres nnn 32 Setting the program End tlme itid aaa iaia Fave eee a 32 Delay SeN ascii llei decida 33 Process End time memory 0 22 drea lei eee eee 33 Start from step different from the first enne enn nn nennen enses 33 Starting a process program iaia viali alia ea 33 Interrupting Suspending the process 34 Opening the SPC lid during a process sssssssssseeeee een narrar 34 Instrument auto teSts 34 Process completion ee 35 Purging Me SPO ariera arenae ea ERR 35 Advices and suggestion for processing i 36 A y ane ee eee cra eee rte peter eee cere eee A ener mene mete reer eer E ey 36 Vacuum and Pressure iii 36 ae a E alianti 36 MINO mem 36 Example of processing programS cnn ttrt ttrt ttt RR RR RR RR RR RR enne rr EESE EEEE nnn nns 37 Short time process small samples oooooooinnncnnnnnicinnnnacoccnnnonannnn nono cnn n nro rca rn 39 gt RN 39 Alcohols dilution so A ADA Ar 40 Graphs of the last 30 processes executed ii 40 Alerts add Alarms Di indu RA TO TR 42 Managing Alda Zi A lia 42 Procedure for the instrument reset nn nnnn nn nn enamore nn cnn ran nnn nn rrer erst nnn n nens 42 Graph of the last 30 DrOCeSSOS rrt ttn ie Qi ua adenda asada danna 43 ALERTS NO T BEOCKING amp iactant
85. proceed to the next step If the problem persists the process will be stopped CAUSE Very low quantity of reagent in a bottle reagent bottle quick connector not fully inserted into the female receptacle SOLUTION Check reagent bottle position and fluid levels If the problem persists contact our service department for technical assistance EXPLANATION The rotary valve is out of position CAUSE The Rotary Valve motor failed or the Rotary Valve position sensor failed SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION An essential computer file is damaged or cannot be found and the error recovery system couldn t correct the problem CAUSE Computer malfunction CSQ Rev 120416 of 16 04 12 Page 48 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i SOLUTION No intervention by the user is possible Please contact our service department for assistance EXPLANATION The SPC chamber temperature was too low during the drain of a wax step the drain cannot be completed The process will be stopped to avoid other problems The samples will be left in wax till the user intervention CAUSE Incorrect working of SPC temperature sensor
86. r technical assistance EXPLANATION The process has been suspended for the opening of the SPC lid or by user request CAUSE When the SPC lid is opened during a process or the user suspend it by pressing ESC and ENTER the computer temporarily halt the process execution When the SPC lid is closed back the computer automatically resume the process execution within 3 minutes from the opening The user can also manually resume the process if the lid is closed by pressing the key ENTER SOLUTION Close the SPC lid as soon as possible If the process is not resumed wait for the system to automatically check the SPC lid micro switch functionality that it will happen within 3 minutes from the lid closing If the micro switch will be found broken the system will automatically disable it and it will resume the process execution Of course meanwhile the SPC lid must be left closed If the SPC lid is closed but the system shows that it is open the micro switch that controls the SPC lid status is surely broken or out of position Waiting for service it is possible to manually disable it by the instrument SETUP menu EXPLANATION The process has been suspended indefinitely cause the opening of the SPC lid CAUSE in case of process suspension the process MUST be resumed within 3 minutes after that time the suspension is considered accidental and this ALERT is issued to not confuse it with alert 20 SOLUTION see ALERT 20 EXPLANATION
87. r selectable from 5 to 30 frequency RFD Remote Fill Drain For reagents and purge agents Data backup On USB memory User interface LCD 15 colour monitor with Touch Screen Process Programs 12 including one for reverse processing Delay Up to 14 days 23 hours 59 minutes Process End Time management For every process program with auto memorization Purge 1 factory optimized Charcoal filters 2 easy substitution and safe handling Password User selectable multilevel Power failure device In the event of a power failure the processor restarts the process from the interrupted point csQ MED ISO 13485 Rev 120416 of 16 04 12 Page 59 of 60 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano 1 O a fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 4l www bio optica WWC Wax Warm Chamber SPC Retort Sample Processing Chamber RMS Reagents Management System WCC Wax Cleaning Cycle RFD Remote fill and drain system HPa Pressure unit of measure W Power unit of measure Watt A Current unit of measure Ampere V Voltage unit of measure Volt The product features reported in this manual are subject to change without notice VTP300 Service manual Touch Screen Monitor User Manual
88. rapidly removed and easily reinstalled in their housing slots The control devices hardware and software are based on the most up to date processing control technologies Up to 12 different programs can be permanently stored and be easily modified The 12 program is a special REVERSED program that is used to de process samples that have not had good infiltration During a run every step and action is displayed on the screen such as current step and function emptying filling etc processing chamber and wax heating chamber temperatures processing chamber pressure completion time and date and any other parameter necessary to simplify the use of the instrument In the event of power failures the computer saves all the data necessary to restart the process exactly where it was interrupted If the interruption happens during the wax stages particular precautions are taken to guarantee melting before any wax filling or emptying begins The user interface is based on Touch Screen technology Therefore there isn t any traditional keyboard all the instrument functions are activated by a finger touch At the bottom of the screen there is a window with a green background colour used for messages to the operator Those messages inform and guide the operator about actions that can or must be taken to safely operate the instrument In the upper left side of the screen there is an icon with the letter i inside by pressing on it a window
89. s purposely longer than the slot in the lid The length excess must be distributed on all slot sides The insertion is normally possible with a little bit of patience after one or two attempts eso CSQ Rev 120416 of 16 04 12 hs Page 55 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano E O P fi CQ Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The VTP300 with 4 USB ports in the back panel To one of those ports any kind of USB printer can be connected the system should promptly recognize it through plug and play functions If that does not happen please call our technical service it may be necessary to manually install a software driver General description The UPS is an optional The VTP300 can be powered by an external UPS connected or not connected to the VTP300 by the RS 232 the difference consists in the following Connected UPS functions and advantages It can communicate with the VTP300 to manage the situations of power failure It reduces the power consumption in case of power failure lt will avoid operations of fill and drain when the batteries charge is low During a power failure it allows the continuation of the processing for a time between 30 and 60 minutes Thanks to the communication it is also possible to always know the situat
90. ssible to fill the content of a bottle into the SPC The bottle will be jumped and the following bottle in the process list will be used the time of the aborted step will be added to the current one If the attempt succeeds the process can continue without problems In case also the following bottle cannot be filled the process is interrupted and the instrument indicates alarm 53 LINE CLOGGED CAUSE full clogging somewhere in the part of the hydraulic circuit of the jumped bottle SOLUTION Verify where possible the bottle pipeline EXPLANATION file not found or corrupted in the long term computer memory the systems recovery replaces the lost or incomplete file from a backup copy CAUSE transitory malfunction of the CPU memory SOLUTION No intervention by the user is possible If the problem persists contact our technical service EXPLANATION the time required to drain the SPC was excessive CAUSE Insufficient pressure in the SPC due to not fully efficient air pump an air leak in the pneumatic circuit or an air leak from the SPC lid gasket SOLUTION Check the SPC lid gasket contact our service department for other causes EXPLANATION during the process before the first wax step the temperature of the wax chamber dropped CS Rev 120416 of 16 04 12 Q Page 44 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax
91. start automatically it can be started manually by selecting Start Wax Purif Cycle on the RMS Setup menu If a process has been performed it is important to run a purge cycle before the WCC start From the RMS Menu it is possible to print the following information RMS status RMS settings It is always advisable to print the RMS settings and status before and after any meaningful change made on it A printout of the RMS status can be useful if there is a doubt that something didn t work properly during the reagent substitution and a check of the reagent situation and relative limits becomes necessary Using the RMS Using the RMS is certainly simpler than setting it It will be sufficient to follow its instructions when after the execution of a process and the purge and if any of the predefined limit has been exceeded the RMS screen will automatically appear The RMS can also be accessed manually by pressing RMS from the START Menu io plica agent Man eagel Mai agen iagement System nder Execution DAF UM 0 200 300 0 0 20 200 ae 00 s E 6 090 200 00 i T 0 20 300 U P 0 200 300 20 200 300 i N 50 300 200 300 DO E L AENA 80 200 300 001 NC Y 3 WAX 60 120 200 300 001 NO i b x ali si oe E l j 5 1 ila wale A 4 ID 0 0 i 0 N a LI ID c lt p emma is palo SI MED IDA anne ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via S
92. t can be on some extent modified by the user It can be started anytime from the Main Menu however at the end of a complete process the purge start is automatically requested by the system it can be jumped by pressing the ESC key but if a wax was loaded during the last process the following process start will be denied until a purge is performed Press ENTER to start the purge As well as in any other program the purge cycle can be aborted by pressing ESC and then ENTER within 3 second The bottles containing the purge reagents are labelled with P1 and P2 and must contain P1 xylene or substitutes the second P2 95 or 10096 ethanol The reagent must be substituted every 3 to 9 purge process see sub chapter Purge reagents limits setting The removal of wax residues before the start of the purge program is useful to extend the life of the purge reagents Avoid the use of sharp tools that can damage the processing chamber surface Even if the processing chamber is maintained at the last wax temperature till the execution of the purge cycle it is preferable to run the purge cycle immediately after the process The purge total time duration is 64 minutes The last purge step is the drying of the processing chamber from any alcohol residue This step is not critical and can be stopped at any time without damaging the instrument Jumping the drying step the purge total time is reduced to 34 minutes se Rev 120416 of 16 04 12
93. t interruption between 0 1 and 0 5 seconds can be much more harmful than a prolonged interruption We highly recommend the installation of a UPS when the main electrical power supply quality is known to be poor and the interruptions are frequent Switching the UPS on and off To switch OFF instruments equipped with a UPS it is not sufficient to turn OFF the main wall switch or disconnect the UPS power cord The UPS will keep the unit powered also with the power cord removed To switch OFF the VTP300 it is necessary to switch OFF the UPS and disconnect the VTP300 from the UPS supply power cord To switch ON the VTP300 it is necessary to reconnect the main power cord to the UPS and switch ON the UPS In case it would be necessary to open the rear panels of the VTP300 to operate on the internal parts we CSO cso Rev 120416 of 16 04 12 hs Page 56 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O a i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i recommend taking particular attentions to ensure the real power disconnection from the UPS The simple switching OFF of the main power wall switch and or the disconnection of the power cord may not be sufficient to ensure that the VTP300 is not electrically powered To ensure that the VTP300 is not electrically power
94. t to the main power The power connection must provide a voltage compliant to the voltage indicated on the label in the back of the instrument and the ground It is recommended that the instrument is plugged into a wall socket equipped with Ground Fault Circuit Interruption GFCI protection as an additional electrical safeguard In addition to the VTP300 anti blackout feature an uninterruptible power supply can be utilized to provide power in the event of power outages and some protection against power fluctuations line noise and power spikes It is highly recommended that the instrument be operated away from heat radiators stoves direct sunlight etc and moisture sinks drains etc Check list for using the instrument mU litial stage set up Check system time and date Set reagent and protocol names Check and set SETUP parameters Setup the RMS parameters Set protocols Install charcoal filters Fill wax and reagent bottles In a a a a a a a fore starting a process Check wax and reagent bottle levels Check the processing chamber for cleanliness Insert samples into the processing chamber Firmly close the processing chamber lid Select the desired program Enter date and time of program completion include a delay function if desired Enter the number of cassettes processed DOCODODOO Follow the instructions to empty the last reagent Wait for complete emptying before opening th
95. t useful and efficient remedy is the first wax step time increasing Another sign of this problem can be the systematic decrease of the level of the first wax bottle together with the increase of the level in the second wax bottle The overall samples fixation is normally done outside the tissue processors That is due also to the need to perform different fixation type and timing in relationship to the kind and size of sample The first step in formalin is not necessary if the fixation has been completely executed outside the tissue processor It can be useful to remember that the formalin can leave solid crystals that may be detrimental for some of the internal parts of the tissue processor and again this is worth for every kind and brand of tissue processor Thus we recommend what follow a Perform a complete fixation outside the tissue processor Wash the samples in fresh tap water before to introduce them in the tissue processor Set for the first step a low gradation alcohol or water In case the fixation is completed in the tissue processor set a bottle of water in the following station that is worth especially for week end processes O N Ooo cso Rev 120416 of 16 04 12 Page 39 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano E O p i ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendi
96. te 39 02 21 53 000 Ww ww bio optica i More or less in every kind and brand of tissue processor the alcohols are subject to a forced dilution due to the water released by the samples that is the work done by the alcohols water removals from the samples or dehydration This unavoidable dilution is exploited in the RMS by Group to avoid the need to dilute the alcohol before their loading in the bottles In the processing program examples previously shown only 95 and 100 alcohols are used implying that a The tissue processor would be used with the RMS activated a Only at the very first alcohols loading the first three would be manually diluted in order to create a gradual increase of the alcohol concentration for example 50 80 95 theoretically only once in the life of the tissue processor practically every time for any reason it would be necessary to substitute all the reagents and reset the RMS a Please note that what suggested in point 3 is advisable but not strictly necessary because in several laboratories where the VTP300 is installed are used 95 and 100 alcohols without ever performing any dilution and with very good processing results At the end of the process it is advisable to look at the graph of the last executed process in order to check if it was performed flawlessly We recall that to access the graph is it simply necessary to press the key GPH in the Main Menu screen the graph firstly shown is always t
97. the SPC at the end of a process The lid must be opened with care to avoid the possibility of eye injury by reagent vapours and splashing Always wear safety glasses when handling reagents Fuse replacement should be done only by qualified personnel Please note always disconnect the instrument from the main power line Never use fuses of a different rating and never try to repair damaged fuses The VTP300 is equipped with two line voltage fuses Fuses are placed into fuse holders located on the rear panel Rating 100V 12 5A 110V 10A 125V 10A 225V 5A 240V 5A 6 3x32mm T After the instrument is powered if a fuse blown again do not try to substitute it another time A possible main electrical failure is undergoing in the instrument Call our service department for technical assistance eso CSQ Rev 120416 of 16 04 12 hs Page 53 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O na fi CQ Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The socket of the Remote Alarm connector has 3 contacts with the following disposition Noalarm contacts 1 2 closed contacts 1 3 open Alarm active contacts 1 2 open contacts 1 3 closed For the location of the Remote Alarm connector please refer to the paragraph The Rear Panel at the beginning
98. the UPS no process can be started because the UPS autonomy is limited to 30 or 60 minutes depending on the charge status of the batteries The SPC lid is heated independently from the SPC and WWC heating to avoid the building up of condensation underneath the lid when in the SPC is present a warm reagent the condensation will then fall down when the lid is opened creating an uncomfortable situation for the user The efficiency of the heating is sufficient to avoid most of the condensation only a few drops of reagent may still be present in the peripheral zones of the lid especially when the reagent inside the SPC is warm and the outside ambient temperature is particularly low under 20 C The temperature of the inside lower face of the SPC lid may reach 54 60 C so it can be uncomfortable at the touch but it is not anyhow dangerous The limited power of the heating element 22W compared to the mass and size of the lid makes it impossible the creation of dangerous temperatures Any case a safety thermostat 80 C max has been added to the heater to prevent any kind of over temperature ese CSQ Rev 120416 of 16 04 12 v hs Page 54 of 60 MED ISO 9001 ISO 13485 e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano T O fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica it The daily maintenance of th
99. tica i fe k Once the RMS is activated it is not anymore possible to consider the physical position of the tanks or their position in the program STEP It will be necessary to fully trust the instrument and follow its instructions regarding the substitution of the reagents When there is a doubt that something is not working properly for example in case of poor tissue quality it will be necessary to replace all the reagents and use the counters reset functions to reset the counters In this case it is recommended to reconsider the RMS settings and decrease the limits until a substantial tissue quality is obtained It is not possible to foresee a RMS standard setting The RMS ideal setting is strictly related to the kind of usage done with the VTP300 type and quantity of fast short and slow long processes performed type of reagents type and quantity of samples processed that ideal setting may be experimentally found after a few weeks of continuous and regular use of the instrument In order to obtain a good reagent management it is necessary to set the RMS not only in terms of type of reagents used but also in terms of predefined limits that will trigger the reagent substitution set for each reagent tank In the VTP300 the limits are based only on the number of cassettes processed Those limits will be defined for each tank of reagent see next chapters it is here important to describe how the limits will be handled by th
100. time will increase Delay setting By pressing on the fields their value can be changed The computer will automatically update the total processing time the date and time of completion and the fields that indicate the amount of the delay expressed in days hours and minutes The maximum delay is 14 days 23 hours 59 minutes It is not possible to set an End time lower than what will result by adding the total process time to the current time Any delay programmed will extend the length of time the specimens will remain in the first reagent Process End time memory A convenient feature of the VTP300 is the automatic recall of the End time gt Every time a delay is requested or updated thus every time an End time different from the natural one is set the computer stores the resulted End time it in the long term memory gt When a program is started the computer will propose recalculating the delay the same End time set during the previous start of that program If no delay was set the computer will propose for the End time the natural time current time total process time gt The delay necessary to end the process at the predetermined hour and or day is automatically calculated and displayed in the appropriate display field gt To reset the delay press CLR The delay will be set to zero and the delay memory will be erased The next time this program is started the End time will be the natural one current time total proc
101. tino 58 e 20134 Milano 1 O Lar fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i Modern LCD screens life is quite long but the lamps that provide screen s lighting are characterized by a much shorter life For this reason a screen saver device cuts off the screen power 30 after the last time somebody touched the screen surface A simple touch in any place of the screen surface will re start the power and the screen functions in approximately 1 second In case the screen would not wake up it will be necessary to switch OFF and then ON after 10 seconds the entire instrument from the main switch located on the rear panel If also this attempt will fail there is still the possibility quickly after the switching ON of the instrument to switch ON the screen by its small push button switch which is located on the right bottom of the screen frame It is recommendable to not touch the screen more than once to wake it up the first touch it is not recognized as a command but only as a request to wake up the screen the following touches are recognized as commands and with the screen not yet visible may give unpredictable results O o ETHERNET USB FUSES POWER SUPPLY FAN VGA RS 232 REMOTE ALARM MAIN POWER SWITCH and SOCKET Notes Remote Alarm socket No alarm pins 1 2 closed pins 1 3 open Alarm pins 1 2 o
102. um step after the lid handle opening it will be necessary to wait that the system suspends the process and perform the VACUUM RELEASE before the opening of the SPC lid will be possible The message line will then display Normalizing pressure in the SPC and after 10 seconds the SPC lid can be opened 5 If the processing chamber is not closed within 3 minutes an ALERT will occur 20 PROC SUSPENDED and a beeper will start to sound When the SPC lid is closed back the process is automatically resumed after a while or the process can be resumed by pressing the key ENTER 6 If the opening of the chamber is difficult DO NOT force it wait for the ambient pressure to be resumed Before and during the process the system verifies if there are the right conditions to start continue the work At the start of a process a series of parameters are checked if one of them is not conform to what is required to safely start the process the start is aborted and a message is shown so that the user can take adequate measures to remove the fault condition See also the chapter Safety devices Runtime test During the process another series of checks is performed continuously to ensure the safest sample processing For example before to fill a bottle the filling pipeline is tested to ensure that there isn t any clog or reagent of the previous step left in the SPC If the pipeline is not completely open Alert 6 and Alarm 53 are issued that specific bottle
103. uspension A beeping starts to signal the potentially faulty situation to the users This alert is also issued on instruments equipped with UPS when there is a power failure and the batteries charge is not sufficient to keep the instrument alive the process has been suspended and the instrument prepare itself to the complete power failure SOLUTION see alerts 20 21 and 22 EXPLANATION The Reagent Management System is an automatic feature that periodically requests the user the substitution of certain reagents in relationship to pre determined plans The user may decide to csQ Rev 120416 of 16 04 12 Page 46 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 20134 Milano 1 O pma fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i postpone the execution of an RMS request but in this case an alert is issued and stored in the memory for future consultation If the RMS is postponed only once nothing bad can happen to the processing quality but in case of more than once postponing events the quality of the reagents may go under an acceptable level CAUSE The RMS execution has been postponed by the user SOLUTION Execute the RMS every time is requested by the system EXPLANATION The RMS has been postponed due to lack of knowledge of the password See also Alert 30 CAUSE
104. while it is possible to successfully and safely use the VTP300 EXPLANATION during the process the temperature of the sample processing chamber did not reach the setpoint the process continues till the last reagent before the waxes If the problem persists before the draining of the last reagent before the waxes the process is stopped see alarm 60 CAUSE The thermo regulation system of the SPC is out of order or step time too short to allow the reagent to be heated at the desired setpoint when the EHE is not used SOLUTION If the problem is due to a mistaken programming change the step time or temperature If the problem is due to a fault on the SPC thermoregulation system no intervention by the user is possible If the problem persists contact our service department for technical assistance EXPLANATION during the reagent fill the filling time was too short the reagent level may be insufficient to Cso Rev 120416 of 16 04 12 Page 45 of 60 MED ISO 9001 ISO 13485 e e Bio Optica Milano S p A e via San Faustino 58 e 1 20134 Milano O p fi ca Tel 39 02 21 27 13 1 e Fax Acquisti Export 39 02 21 54 155 i p Fax Assistenza Contabilit 39 02 26 41 74 48 e Fax Vendite 39 02 21 53 000 www bio optica i cover the samples CAUSE Malfunctioning of the pneumatic system reagent level in the tank too low SOLUTION Verify the reagent level in the tank s If the problem persists contact our service department fo
105. y select the most contaminated reagent first and the least contaminated reagent last From the SETUP menu it is possible to modify the most important instrument parameters except processing protocols in this menu are located all the parameters that do not require frequent access Here the main parameters can be modified After the modifications by pressing SAVE the new values are stored in the memory by pressing ESC modifications are discarded Any case it is necessary to press ESC to get out from the function In the first row on the top right of the PARAMS SETUP screen the current software version is shown For example SW version 5 5 VTP300 The only relevant parameter for the instrument user is the software version the others are related only to manufacturing internal use data Seven languages are available English French German Italian Spanish Czech and Turkish Date Format In the USA the date format is month day year In Europe the format is day month year Also the time is displayed in a different way Here it is possible to choose the desired format The ambient pressure decreases together with the elevation from the sea level approx 100 HPa less every 1 000 meter for the first 2 000 meters It is important to tell the instrument what is the level at which it is installed to avoid it to require the air pump to reach differentials of pressure levels of vacuum impossible to make especially when the elev

bio-optica - vtp300 histology vacuum tissue processor user manual

Contents

Download Pdf Manuals

Related Search

Related Contents