Home

ArcturusXT™ LCM System Troubleshooting Guide (4458770A)

image

Contents

1. apertures to make sure they are both fully open Check the filter sliders found in illumination tower that they are not partially pushed in Field aperture not centered Center field aperture Fluorescent cube turret Check to ensure position is fully clicked in Magnification Tube Check to ensure position is fully clicked in Remove DIC sliders located below each objective Note Replace empty slot with cover to prevent dust from entering Background of live image not clear white Re establish white balance in camera properties dialog located under View in the pull down menu l A 2 Fluorescence cube in place Check fluorescence turret If using brightfield illumination it should be in an position not containing a filter cube Slide overview image too dark bright Settings not optimized Change magnification to 2x and adjust the brightness setting Right click in slide overview area and select Remember settings in pop up window select Yes to save for all future overviews and finally select Require Overview Arcturus LCM System Troubleshooting Guide 1 PN 4458770 Rev A 07 2010 Fluorescence Fluorescence Image fully or partially blocked Fiber Optic Cable Fluorescence Lamp Phase Contrast DIC Fluorescent Field Aperture Fluorescence ND Filters Fluorescence signal suboptimal long exposure Fluorescent ND Filters time required DIC or Phase Optics Ensure that the fluorescence field ape
2. Please contact technical support to arrange for service Fire IR laser using the test fire button located in the Microdissect Tool Pane Move around under the cap to locate wetted spot Right click in the center of spot and select IR spot located from pop up window Tissue is thick or dark Move to a thinner tissue area or a clear area on the slide IR Capture laser fires off target IR laser location set incorrectl Relocate IR laser See Section 7 6 Locating the IR Capture Laser UV Laser Cutting UV Laser does not cut Neutral density filters may be in place Verify that the ND filters are in the out position levers tilted to the right The ND filters are located behind the front cover of the UV laser housing on the left side of the instrument Tissue is thick or fibrous Reduce UV cutting speed located in the Microdissect Tool pane UV Cutting is inefficient Cutting speed Reduce UV cutting speed using the slide bar located in the Microdissect Tool Pane Long lag time when updating the live image Light intensity and or camera gain settings not optimize The Brightness setting located in Microscope Tool Panel should or gt 0 200s Open the Select dialog box and re adjust intensity and or camera gain to achieve an appropriate brightness setting Brightfield image fully or partially blocked 1 Apertures Field and or Condenser If you have the Nikon phase contrast illumination tower check the field and condenser Image Qualit
3. not aligned to markings of drawing item s Magnification of image does not match software operation UV and or IR lasers not located properly Brightfield light is flashing either slow or fast Stage bump Limit for cap robot arm exceeded Check position of Intermediate magnification dial and compare against magnification tab located in microscope dialog box Both of these items should match at 1X or 1 5X See Section 5 4 Using 1 5X Magnification Perform IR and or UV locate found in the microdissection dialog box See Sections 7 5 Locating the UV Cutting Laser and 7 6 Locating the IR Capture Laser Re align stage so that all front edges of the stage are parallel Close down software turn off the Arcturus instrument Restart the Arcturus instrument and re initiate the operating software Lower the cap fork by turning the lead screw counter clockwise 3mm slotted screwdriver until the motor takes hold Close down software turn off the Arcturus instrument Restart the Arcturus instrument and re initiate the operating software Contact Technical Support at 1 800 831 6844 option 5 for more details Microdissection process does not initiate within Live image was moved away from where static image _ Move the cap to the area containing the items for microdissection and click the Harvest AutoScanXT by using the Harvest button was taken button again For Research Use Only Not intended for any animal or human the
4. applied Arcturus Arcturus LCM System Troubleshooting Guide wW biosystems vy Life technotogies Laser Capture Microdissection System Nikon Eclipse T E Microscope Base CATEGORY SYMPTOM POSSIBLE CAUSE S REMEDY Cells do not adhere to the CapSure Cap Inadequate IR laser power and or duration settings Open the IR Settings tab within the Microdissect Dialog box and the adjust power and duration settings until proper wetting is achieved See Section 6 6 Setting the IR Capture Spot Size for complete instructions Tissue preparation IR Laser Capture LCM Debris or loose tissue may be present Use a PrepStrip to remove the debris or loose tissue from the slide prior to microdissection Folds may be present Position the Cap such that it is not placed on the fold Alternatively increase IR laser power and duration settings to achieve proper wetting Tissue not dehydrated Ensure that the section is properly dehydrated Proper dehydration 100 ethanol for 1minute followed by Xylene for 5 minutes and then air dry for 5 minutes Cannot locate IR Laser Bias and or brightness settings too low Open the IR locate tab within the Microdissect Dialog Box The Bias setting should be at 60 You may also need to adjust the Brightness setting found in the Inspect Tool Pane to darken the field of view and visualize the IR laser guide light IR laser is out of the field of view IR laser location needs adjustment service is required
5. rapeutic or diagnostic use The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners Nikon and Eclipse are trademarks of Nikon Corporation EXFO is a trademark of EXFO Inc 2010 Life Technologies Corporation All rights reserved Headquarters 5791 Van Allen Way Carlsbad CA 92008 USA Phone 760 603 7200 ABES FAD biosystems www lifetechnologies com Arcturus LCM System Troubleshooting Guide 2 Technical Resources and Support For the latest technical resources and support information for all locations please refer to our Web site at www appliedbiosystems com PN 4458770 Rev A 07 2010
6. rture is centered Fluorescence ND filters should be in the out positions he shutter on the fluorescence turret should be open O position ND fluorescent filters should be in their out positions The DIC analyzer amp polarizer should both be in their out positions The Condenser position should be set at A Check the fluorescence adapter cone to ensure it has been attached properly A Ensure that the fiber optic cable is fully inserted into the cone and the EXFO control box Check the position of the fluorescence lamp to ensure it is properly seated See the EXFO user guide Check the lamp hours Lamp life 2000 hours The phase contrast image does not appear The diffuser optimal Phase annulus Annular diaphragm The diffuser The DIC image does not appear optimal Condenser turret position Polarizer and analyzer Condenser alignment General Instrument Ensure the diffuser is in the Out position in the Select Options dialog box Ensure the proper phase annulus is chosen for the objective in use PhL 4x Ph1 10x and 20x Ph2 40x and 60x Ensure the annular diaphragm is centered Ensure the diffuser is in the Out position in the Select Options dialog box Ensure the condenser is turned to the DIC N1 position Ensure that the polarizer and analyzer are both fully pushed into their IN positions Ensure that the condenser is properly centered and focused Microdissection UV and or IR

Download Pdf Manuals

image

Related Search

Related Contents

Sony VGN-NW220F/B Quick Start Manual  120-102_Digifold service Manual.cdr  MD 9272 Leakage Clamp TRMS Meter with Power  All in One - Active System Integration  L`APPROCHE PROCESSUS,  Electrolux ERF 2830 User's Manual  Samsung Xpress C460FW Цветен Мултифункционален Принтер (18 / 4 стр.за мин.) Наръчник за потребителя  Husqvarna 125/2002 Motorcycle User Manual  Rester Jeune - Maryline Hourlier  View the manual  

Copyright © All rights reserved.
DMCA: DMCA_mwitty#outlook.com.