Acquiring and Scanning Images Setting up Monitor Troubleshooting
Contents
1. Move lettering by moving cursor outside lettering area Edit lettering by dragging over lettering to highlight first Each new click of the Text tool makes a new layer Changing Orientation of Type Edit gt Transform gt Rotate Drag from a corner Hold down Shift key for increments Arrows Scale Bars Select Line tool on Toolbar double click on itin 4x 5x Click on arrowhead on menu or select A rrowheads in Palette 4x 5x Width is 400 or so Length 600 or so Concavity 0 Weight is set for thickness of line in pixels Scale Bars Draw line sans arrow heads while holding down Shift key amp checking Info Box for desired length in pixels Boxes Circles M ake new Layer in Layers bos Choose M arquee hold down for Ellipse Draw rectangle for box or circle hold down Shift key Edit gt Stroke Set Width to desired thickness Common Shortcuts Zoom Control Control A ppleK ey amp Navigate Spacebar M ove Tool Control Apple K ey when on Marquee tool F 7 To reveal Layers Box F8 To reveal Info Box Copy Control C key Paste Control V key In Image Size dialogue box Check Resample when going to Publisher Photographic Printers Uncheck Resample when going to PowerPoint Ink Jet Laser J et Slide Writer Crop Layout then set desired Width and H eight Saving Files JPEG PowerPoint Word PSD Photoshop for Re Editing Purposes TIFF For Publisher PDF For Distr2. Acquiring and Scanning mages 5 Steps to Follow When A cquiring Digital Images White Balance Color Images Only Brightfield Set K oehler illumination on microscope B rightfield Correct for Uneven Illumination in the Field espe cially at low magnification lt 10x Avoid Over Saturation fluorescence primarily Grayscale with text Binary Image or Frame Average when possible for fluorescent or dim images Setting up M onitor 1 Using controls on your monitor itself adjust brightness so that it is at the halfway point and contrast so that it iS all the way up as contrasty as possible Keep ambient lighting LOW 2 If not done already set up computer screen for color and contrast using A dobe Gamma Windows 1 Click on Start 2 Select Settings C ontrol Panel 3 Double click Adobe Gamma 4 Choose Step by Step Wizard See steps at right for details 5 When setting gamma choose 2 2 Europe 1 8 Troubleshooting Wrong Layer Text Layer Wrong M ode File Size Settings when Scanning Color Scans without text 6 8 megabytes Color Scans with text 10 megabytes Grayscale without text 2 3 megabytes 4 6 megabytes Ignore file size and set t 1200 2400dpi the smaller the text the larger the dpi M ac 1 Click on Apple symbol upper left 2 Highlight Control P anels Adobe Gamma 3 Choose Assistant 4 W hen setting gamma choose 2 2 Europe 1 8 Cannot complete re
3. crop tool Under View uncheck Snap To gt Document B ounds version 7x Dust amp Scratches Filter gt N oise gt D ust amp Scratches K eep Radius at 1 or 2 Use Eyedropper tool for larger areas Healing tool in 7x Choose fuzzy edged Brush in menu for 6x 7x open Windows gt Brushes in 4x 5x Be ethical Consult with your advisor Sharpen Filter gt Sharpen gt U nsharp M ask M oveA mount slider keep Radius between 1 amp 2 Color Correction mage gt A djustments gt L evels Click white eyedropper tool on white background for Brightfield images or use A uto Color Image gt A djustments gt A uto Color in 6x 7x Color Adjustments mage gt A djust ments gt H ue amp Saturation Start by desaturating offensive colors Shift blue to cyan for fluorescent images Color M atching 2 or moreimages Use Color M atching protocol Balance backgrounds first Use location marker Eyedropper Tool Shift key to mark similar points on 2 images in the Info Box Use Curves Image gt A djust ments gt Curves amp pull down or across from top to match RGB values Color Adding fluorescence Image gt A djust ments gt Curves Select color from Channel OTHER than desired color Pull down line from top to bottom quadrant blue should be pulled further to add yellow to green or red be sure gradient at bottom of dialogue box is clicked on to be dark on the left Adding W hite Space to Images for Lettering gels etc Image gt Canvas S
4. ibu tion Grants
5. ize Putin new values Be sure background at bottom of toolbar is white Common Steps for M aking M ulti l mage Plates amp Working With M ore Than One Image M erge I mages Fluorescence Colocalization Open red amp green image SelectAll on red image cut close don t save Paste on green image In Layers box select Screen vs Normal from drop down list M ake New Layout Page File gt New Typically image dimensions are best determined by making a letter sized page 8 2 X 10 7 allowing for margins at 300dpi Copy and Paste or drag images onto this page using M ove tool or M arquee tool control command key Be sure that images are flattened Layers gt Flatten before moving or that L ayers are locked with lock icon Scale mages Edit gt Transform hold down Shift key while re sizing by clicking on a corner If images are not on aL ayer these will have to be selected with the M arquee tool first Images can be scaled similarly by paying attention to scaling percentage in the Info Box Lining up Images M aking borders Drag down across guidelines from Ruler Area M ake borders between by zooming in to create 10 20 pixel borders Preferences gt Units amp Rulers gt U nits Pixels Under View gt Snap or Snap To Guides should be checked Lettering Click on T tool in 6x 7x Double click in 4x 5x Settings for tool are in Character Palette on menu 6x 7x Click check mark or return to M arquee tool to finalize 6x 7x
6. quest because the file layer is locked or Cannot complete because area is empty You are on the wrong layer or on the background layer in 6x 7x Computer crashes often in Photoshop Scratch disks are full In Preferences choose primary amp secondary disks with lots of hard disk space 3 times the size of the file Defragment these disks regularly Crop tool magnetizes to the edge Use M arquee tool instead 4x 5x or under View uncheck Snap To gt Document Bounds Crossed line over circle icon appears M enu selections are grayed out Y ou are probably on a text layer select the layer of interest It can also be a result of the image being 16 bit or Indexed Color change the M ode Image gt M ode gt RGB Color or 8 bit used a function such as L evels but nothing happens You have accidently drawn a tiny selection with the M arquee tool control command key D deselects or you are on the wrong layer Line tool doesn t makea line M ake sure the foreground color in the toolbar is not the same color as what you are drawing over M ake another layer in 4x 5x a bug in these programs In 6x 7x render the layer Layer gt Rasterize No pixels are more than 50 selected The selection edges will not be visible You are on an empty layer choose layer of interest or you have feathered your edges or chosen a Fuzziness value in Color Range too radically Setting Preferences Preferences gt General g
7. t Transparency amp Gamut Set Grid Size to None gt Display amp Cursors Set Painting Cursors to Brush Size gt Plug Ins amp Scratch Disks Set 2nd if you have additional hard drive or partition Preferences gt Color Settings Uncheck Ask When Opening to undo color mismatch prompt View gt Show Rulers or Rulers Setting up Info Box Ko 37r a R 160 231 E 160 231 K Previous black ink amount Current black ink amount Click on arrowhead and select P alette Options Hen First Color Readout Set F irst C olor mode Grayscale Readout to Second Color Readout G raysca amp Mode Actual Color H Set Second Color Mouse Coordinates Readout to Buler Units Actual Color Common Steps for Single Images Typical M ode M ode may have to be changed to Grayscale or RGB Color 16 bit to 8 bit Image gt M ode Levels Image gt A djust ments gt L evels A djust contrast with white and black sliders middle slider if image is M ove Output black slider so that K value in Info box reads at gt 90 usually 85 white Output slider is at not evaluated by light to dark values as in gels blots and images intended for densitometry 250 254 unless the image is a graph or drawing Scanned images Align Drag down guideline View gt Show Rulers drag from Rulers Image gt R otate Canvas gt A rbitrary Rotate or Flip Image gt R otate Canvas gt F lip or 180 90 CW CCW Crop Use
Download Pdf Manuals
Related Search