User's Guide
Contents
1. a g Q Survey Scan a i ele we dawnt mm cdowwbwwnk coche ce Dac a Bec Ue ew bi hee alee ee a Pe eee sey ese ge ee ee es nk na nima aia a es e o e es ee ers an iiad bokalak Salai ical aNd as ly lady aaa a ale we dem eda wcdewwb on nb nnn ne Jeena ce hc ao le ele el ee ee a de cede eed a web oe ok oa ale oe hee eB a Ee ee a St i a or ee ge eg ee ee Ga ad Ala lita Naat Akaia Nada peli attain Tdi aa a ae al oy a ay yy G Measure ll tne NAME SUR NewMethod STARTWAVE 200 ENDWAVE 400 i STEP 5 a i SPEED NORMAL Wave Title 3 Title 4 2 149 2 481 1 an DAAF Title 1 Title 2 0 796 1 561 near 200 0 one N 1331 3 Measure 1 Select cell number A 2 Input Name Start wave End wave Step Scan speed and so on B 3 First blank must be scanned with selected condition as order 2 Click Baseline C for setting up for blank After setting up with blank all cleared to measure 4 Click Measure D 4 Graph Setting E Arrows Set Peak Valley Cursor Peak Valley Set Range Absorbance Transmittance e Set Range 1 i Property Window Set min max wavelength y axis and absorbance or Min of 0 5 Max of 4 Min of x 500 Max of x 600 transmittance x axis v Apply 1x Cancel e Set Peak Valley 2 i Property Window This is to set the condition o2. File Config Window Help ATC Open Ctrl O Survey Scan Save Ctrl S Kinetic Save As Multi Wave Export to Excel STC Manager Print exit Select measuring mode at File F New N or select New icon in tool box e Open Save File Save as Open File Files of type OptizenView Data File 0DF Cancel 2 y Interface of File saving and opening mostly like designed operated same way as Windows e Open File F Open O or select Open icon in tool box e Save File F Save S or select Save Save as icon in tool box X Data files are saved as ODF file e Export to Excel E Using Export to Excel E you can save as MS Excel csv form 11 U e5 s r 04 U09 21Sseg puy nuaw z EAA AEE E AI EI AET U fa ml 04 U09 21Seg puy nuaw z EA EAEE E AI bola AENT 12 Print P File F Print P or select print icon in tool box You can choose the report form among Graph Table Graph or Table ti OptizenView 4 2 File Config Window Help 3220UY New gt STC Manager Open Ctrl 0 Ctrl 5 Save Save As Export to Excel 3 Chart Table ga Chart ga Table Mode selection and File management Move cursor to left side of display of OptizenView New Open And you can see modes display as below i OptizenView 4 2 File Config Window Help DO lg amp gA 7 STC Manager 3
3. Basic Method C gt ATC Survey Scan T gt Kinetic Scan While execution Measuring appears on Message Box F and after execution Ready appears on a message box and measured value will be displayed as a table and a graph undp Map D Multiple Wave Scan Double click Survey Scan Poly yybuayaaen dIJl9ads uj 29ue WSUeI 2ueqosqy bulunseay e o KILARSA lee mere Poly y u l neM JIJldads uj 99ue NWWSUeI 2ueqJosqy bulsnseay e o KIRUSI ou ele 44 49 5 4 Measuring Absorbance Transmittance Timely 2 Input Name of Test Wavelength Total Time and Time interval C OptizenView 4 2 Measure Al File Config Window Help ipa NAME KIN New Method IE ethod p aE PQ E E E TT yy A Yy E 600 Se Geek cibar vE 0 Hi T TOTAL TIME 00 01 00 oi Kinetic S j A INTERVAL 00 00 10 E FACTOR 3 Click Auto Zero D to correspond the light intensity between Sample amp Blank a Measure amp MERE nny za WAVE 60 2 gt MEASURE H Auto Zero TOTAL TIME 00 01 00 U g U X INTERVAL 00 00 10 a 5 2 i FACTOR 1 While Auto Zero is executing Executing Auto Zero appears on Message Box F after i agg eee 3 Z g neon eae SBOE NOD eee appear S S 7 4 Select Cell Number CELL 1 No 1 B D G D B 4 J m v1 on A Time A r 6 fs al 2 z D D N i i n
4. melee 34 39 4 Attentions while Multiple Wave Scan 5 Quick Guideline 1 The variables used in equations are not case sensitive 1 Introduction 2 You cannot input at Table E directly It will just show settings This chapter shows you measurement steps of each mode step by 3 The variables not defined or out of range are determined as 0 step to use OptizenView more easily 4 In case of errors in equations the value will be determined as 0 2 Confirmation before measurement ti OptizenView 4 2 File Config Window Help wal Cell Type 5 Calculate C1 first and C2 C3 in order 6 You can use C1 at C2 and C1 and C2 at C3 Click Config C Options to check device name type of cell 7 If the wavelengths of STC and Wave list are different it will obey the wavelength of Wave list iD device information and other settings before measuring Q Options N 8 These are examples that can be used at C1 C2 C3 A N E Advanced Setting 5 AM S S A1 A2 F S 3 lt A1 A2 Options Set device name select Cell type lt z A1 1 234 A2 3 141592 TE And set the display option and minimum A A1 A2 A3 yT Decimal 2 maximum of measured value ABS T rs K A1 F1 ABS Decimal 3 o D2 Lamp Saye NO D a Al F2 Lamp Change 370 Ex set device name a T S1 S2 Initial Wave 600 Stand Alone Ver localhost D gt A3 A2 S1 F3 nan oF Area PC Ver PC name or IP address a Max of 1 999 9 i a
5. diareniene 49 Part Ill Technical Supports 1 Technical Supports Sib ULF Skene NG HAAR HERERO E Eee Paes MEAP RRRR VERE Eee RGAE RLOTE TES belae E 50 U w s MAlAUSZIIdC dnas EAR AEE E AEI AET Part Introduction amp General Information 1 Setup OptizenView 1 Introduction Personnel computer can be used with installing OptizenView into your personnel computer 2 Before Starting OptizenView is the exclusive S W for Optizen POP 3220UV 2120UV 2120V 1412V and helps you measure check and control experimental results on the real time in Windows 95 98 NT 2000 XP Vista system It also helps you manage the instrument easier 3 PC System Required OptizenView can be installed with the required system as below e IBM PC Pentium or equivalent e more than HDD 50MB e over 256MB system memory e Mouse and keyboard e VGA Display more than resolution of 1024 X 768 e Higher version of IE 6 0 e MS Windows 98 NT 2000 XP VISTA e Printer Optional 4 Installation e Start Setup exe Find and Execute setup exe at drive E Name amp Size Type C Common File Folder program files File Folder i System32 File Folder Setup 13 507KB Application Caution Above shows drive E however you must check your own CD ROM drive 5 Installation with Install Wizard Process1 OptizenView install wizard prepares to install OptizenView Welcome to the OptizenView 4 2 Setup Wizard This wizard will guid
6. fab 01 u09 DISEG puy nuaw z EAEAN Melo bbe a ATT a 4 Chart Control 3 Configuration y mcoomioue Before measuring for accumulating data check conditions as shown below Bla x Xi hae Survey Scan 1 Cell Type Select Config C Cell Type in menu cell holder types will show as below Select cell holder type then status of changes in cell holder type will appear as below i i i i i i i n ll ee Ae E ole i j oo d co rT O You cannot select some cell types according to instruments ti OptizenView 4 2 File Config Window Help amp 3 Cell Type gt Multi Cell a I i 1 i i Options Single Cell A Change Display Mode Sipper Cell 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 5 Advanced Setting Micro Yolumn Cell a S Nano liter Cell UOICINGIJUOD e NAAA ENE AEAT Use drag and drop will lead display bigger and smaller window E peee Thermal Cell Drag and drop like A right and down in picture above zoom out 101 u09 21Seg puy nuaw z EA EEA ENE E AIEA AET Drag and drop like B left and up in picture above zooms in e Multi Cell Holder Mode Standard cell holder e Move mie 8 rotating cell holder shows as left i vil Keep pressing the right button of mouse and move the graph as your will sgt Me ase Config C Cell Type Multi Cell E ra e Select i i a me Se
7. o 3 UNIT PPM 3 2 n Report z L 3 OptizenView 4 2 gt MEASURE Auto Zero v 5 File Config Window Help oO J J G Dy Alg amp Go STC Manager 3220U a a a A z A w g E vv Basic Method w rom m z z Ja u 2 4 25 2010 10 14 ae 9 ATC _______ ao 3 om g oa O O Cc Cc s3 E zZ lt D D Kinetic Scan gt Multiple Wave Scan Double click ATC 40 41 4 Make STC 6 Select Cell Number CELL 1 No 1 B Standard Curye not defined B Standard Curve E Iv 1 Gos ay 2 5 r4 3 7 After Cell selection Click Measure F to start measurement P gt MEASURE Auto Zero Cone ABS ABS 1 ABS 2 ABS 3 ABS 4 ABS5 STC Memo While execution Measuring appears on Message Box G and after execution Ready U w om U w mi appears on a message box and measured value will be displayed as a table and a graph Name New STC 1 Wavelength 600 0 No of Measurement 3 v i gt Auto Zero gt Measure Type Linear 0 Recalc v Apply x Cancel a Input sample s concentration orderly from number 1 Cell number 1 Number 1 b Click Auto Zero c Click Measure to start measurement d When tests are done absorbance value and data result appeared as graph e Select Recalc and click Apply to use framed graph into ATC 5 Click Auto Zero E to correspond the light intensity between Sample amp Blank ma gt MEASURE gt A
8. then click Type H and Recalc l c If you use multi cell mode the number of cell number and row must be the same For example if you put the sample in cell no 1 3 and 5 you must input the concentration at row no 1 3 and 5 d You can make STC without measuring standard samples Input ABS at ABS column and then click Type H and Recalc I e We recommend that you make the backup file of STC data which is in the folder C Program Files OptizenView 4 1 since STCs are saved on it 8 Calculating value R in a regression line Compensate maximum value of aberration and compute accurate value an equation known as Pearson R equation used when a linear line going through Zero point And other linear line takes method of ordinary least square e Pearson R nl ZX 2X EP AEX Ex a2r rY When X Concentration Y Absorbance e Method of Ordinary Least Square Pa PK Y p O DY When Y Extended coefficient in slope of Selection Curve Y Average 29 SAIND pJepue s pue DNOID 1 SAV PLUS NUE eo ESL U w om uess AdAInS Z IZAERA 0 26 2 Survey Scan 1 Introduction Survey scan allows you to have data with selected cell measured in selected wavelength range After tested and measured absorbance and transmittance test results will be shown as below table and graph 2 Display ti OptizenView 4 2 File Config Window Help DO amp amp E stcManager azz0uv ver 4
9. zi D4 3 im a 28 1145 AR o og 12 02 O O o o D 5 After Cell selection Click Measure E to start measurement D a 1 Locate Mouse point New Open A left side and mode selections will appear as shown E a p P MEASURE gt Autoz R W OptizenView 4 2 z y phate a j 5 Fie Config Window Help r oy a gt 5 i While execution Measuring appears on Message Box F and after execution Ready mi Dy O amp g7 STC Manager 3220u A appears on a message box and measured value will be displayed on a table and a graph z ie g9 A Basic Method z 2 2 ATC 2 T gt Survey Scan D Multiple Wave Scan Double click Kinetic Scan 46 47 5 5 Save and Print Out 6 Trobleshooting e Save e When a message box Instrument is not found appears Select File F gt Save S or Save as A to save 1 Embedded PC Internal PC happens not to be operated in booting process of OPTIZEN Eile Config Window Help measured data 3220UV Please re boot 10 seconds later After beep sound rebooting has been N gt po Sew STC Manager 3220 X File format is ODF succeeded Open Ctrl 0 Select File F Export to Excel E ae 2 Check Device name at Options to save measured data as Excel form Export to Excel Stand Alone Version localhost Print PC Version Optizen3220UV exit 3 Check the OptizenDevice icon shape of mecasys log
10. 220U vv Basic Method ATC updo Hen gt Survey Scan pecs W Mode select Select mode for measuring gt Kinetic Scan T gt Multiple Wave Scan Basic ATC ABS TRA Conc c Optizen3220 T Eca H Program Files E OptizenView 4 2 Folder select Select folder for data 350 2 ODF Potassuim 100421 0DF File select Confirm the saved data then double click will open file 3 Common Function Keys And Input Windows On the right side of display of OptizenView you can see Cell selecting box message box buttons and box of inputting measuring conditions B i m Iv 1 r6 fF all 2 fy Select cell Select the number of cells for measuring 5 3 ra Measure NANE ATC New Method WAVE 600 STC E Set measuring condition Input experiment name and FACTOR 1 g set measuring condition UNIT PPM Report J J MEASURE Auto Zero Measure Autozero Measure amp Auto Zero buttons m m By Message box Windows for status of measuring or error message e Display File Config Window Help D2 BB stcMenager senso vers _ LL ATC New Method F a 1a Eile Config Window Help gt Q amp E STC Manager 3220uv Bio ver 4 7 D _ ATC New Method R ala Standard Curve Click ABS Conc PPM lt ne 2 4 51 2010 10 13 7 13 U
11. Help D 7 g amp Ge sic Manager 3220uv ver 4 0 8 D ENET NAT ke xe VIE Ilj cipa V el ceeeeeecopcecoeeeeceee COCO OCTET ET EEECEXTTEERETE COCO CE CCRC CCPC CECECE ECCS EREEPCAe Seed TB g j E M 1 g Kinetic 4 6 gsi mle wee ee ee ede ee cee cece cele neo o eee LE rs 3 35 Ma E Doe 2 a E a 25 a a EEEE pe SEE ee n wo q 1 5 m eee E d 4 tn nnn ste nn ammm 054 4 4 0220m 0 Measure 2 05 NAME KIN New Method 00 00 00 00 00 10 00 00 20 00 00 30 00 00 40 00 00 50 00 01 01 E Time WAVE 600 al TOTALTIME 00 01 00 Time Title 1 Title 2 s 00 00 00 0 000 0 000 INTERVAL 00 00 10 00 00 10 0 000 0 000 FACTOR 1 00 00 20 0 000 0 000 SU TET TTT aE aeaTT ae A 00 00 30 0 000 0 000 Report v 00 00 40 0000 0 000 D gp pee 00 00 50 0 000 0 000 f b MEASURE I Auto Zero 00 01 00 0 000 0 000 Pecccseseseeeeesee oie PPIE I EP E Measuring Ready i Measuring 3 Measure 1 Select cell number A 2 Input Name Wavelength Total time Interval and so on B 3 Click Autozero C 4 Click Measure D And you will get the results at graph and table in real time 31 U w mi anauiy adus e EIZE E EYA o onauiy aduis e RASTNE o Teel 4 Graph Setting E a la Erie cls amp Arrows Table Set Activity Range Cursor SEI Range Absorbance Transmittanc
12. Min of ABS 0 5 is Max of ABS 4 Min of Wave 190 Max of Wave 1100 4 Initial Value v Apply Cancel 36 37 5 1 Simple Absorbance or Transmittance Measurement 2 Input name of test wavelength Distillation factor and unit C i OptizenView 4 2 PEIES Measure A File Config Window Help me o l NANE ATC New Method _ Q amp gv sic Manager 3220uUv Bio ver 4 10 Hr D q n C ATC New Method ee EET balla ca 1 STe re STC H Standard Curve E ai E _ ses ss FACTOR 1 ff UNIT PPM 3 Click Auto Zero D to correspond the light intensity between Sample amp Blank lt Time Sample Name Wave ABS Conc PPM Remarks gt MEASURE iy Auto Zero Measure Re ae eee c ec cc ccc es ccccccesceceeeseeeceeseccceceseey While Auto Zero is executing Executing Auto Zero appears on Message Box F after execution Ready appears SREP Ene ORR ere A SejectCell Number CELL 4 No 1 B r7 B pi 3 l 6 a i an mer ee sm 1 Locate Mouse point New Open A left side and Mode selections will appear as shown 5 After Cell selection Click Measure E to start measurement ti OptizenView 4 2 BITE b MEASURE U Auto Zero OD E pa amp g5 7 STC Manager 3220U While execution Measuring appears on Message Box and after execution Ready vv Basic Method appears A
13. OPTIZEN VIEW User s Guide For PC Interface software OptizenView 4 2 Feb 2011 2st edition Mecasys Co Ltd Better solutions in Spectroscopic analysis Pi MmeECASYS Notice Spectrophotometer Optizen and Software OptizenView are trademarks of Mecasys Co Ltd No part of the Optizen Series including this publication may be reproduced or modified in any form or by any means without the prior written permission of Mecasys Co Ltd For more detail information on Optizen and OptizenView you may refer to Technical Support in the last Chapter of this guide or visit our websites below http english mecasys co kr Contents Part Introduction amp General Information 1 Setup OptizenView E E E E E aac ne aie tetas heen wae eden eee eaemnen nen neces 1 INtOGUCKION correc EEE 2 Before Starting T E reap E a Raped s ayia eps eye E atin eae E te sen E soe anad aaa 3 PC System Required E E E E E A E A A EAA E E E E 4 Installation ccc EEE 5 Installation with Install Wizard ccc 6 Troubleshooting on process of installation OSER ISEE IEE SEEE E E E ESE 7 Confirmation after installation ccc NO Menu And Basic Control lt r S 1 TOOLD OX cr EEE EEE EEE EEE EEE 2 File Management And Mode Selection ic 3 Common Function Keys And Input Windows oeeeeeeeseeeeeeeeeeeeereeeeettteeeertteeerereereeeen 4 Chart Control ccc EEE EEE EEE oO Configuration E E A a iam aaeeM ens iuenadadecanearedbaqares s
14. TC T gt Survey Scan T gt Kinetic Scan JUdWaINSeay a2uUe IWSUeI JO a UeqIOSgY aduis G EILEAN TEAT oi mele JUdWAaINSeay aduUe IWSUeI JOJad UeqIOSgY aduis G EIZEAN ou meee T gt Multiple Wave Scan Double click ATC 38 39 5 2 Measuring Concentration using Standard Curve 2 Input name of test wavelength Distillation factor and unit D Survey Scan i OptizenView 4 2 F Ed Measure A File Config Window Help NANE ATC New Method Date new Method a aaa WAVE 600 7 Eline cOn A oll aes a i WI 5 Standard Curve E T BE yi ws FACTOR 1 E ie UNIT PPM 3 Pop up Standard Curve window by click Make STC B upper right side of graph Time Sample Name ABS Conc PPH Remarks _ OSEAN TN File Config Window Help F Measure 5 Div lQ amp E STC Manager 3220uv Bio ver 4 amp D y a Me ee T T a pez C ATC New Method a ae 5 la STC F B S Standard Curve E gi E Ea E e T rs r3 L lt M4 D D 7 T To To D D T Time Sample Name Wave Conc PPM Remarks a D gt Measure S lt D D T tenet NANE ATC New Method T I 4 25 2010 10 14 l g UNS I WAVE 600 S a STC d 2 O i FACTOR 1 O 1 Locate Mouse point New Open A left side and mode selections will appear as shown
15. _C 4125 9 32925 9 329255 IPA 9 329255 ipa 9 329255 9 336273 9 336273 LPB 9 336273 PBe 9 336273 lege 9 331175 ese 9 331175 LEG 9 331175 Ec 9 331175 CP 2085 cP 2085 CP 2088 CP r Calibration Type Calibration Type e 2Section C 3 Section C 2Section gt WP LP gt CP v Apply Cancel gt WP LP gt CP v Apply x Cancel Calibration type 2 Section Calibration type 3 Section e WP LP Wavelength and Lamp e CP Cell holder positioning X Attention False calibration by user may cause breakdown 17 U fab UONCINGIJUOD e MAAA ENE AETAT Select item and will appear as shown below 4 Print Out U w J Coie U e5 s r Callers You can print he measured results and you can select three forms Graph Table and This Action will Takes 15 30 Min and Can t be Stop until Completed Do you Want to Continue p p Graph Table oe e Print form Select Print P and you can see three options as below Select one and you can see Pop up process bar and start calibration by click Yes Y preview for it Process Bar OptizenView 4 2 Running Calibration wp Ip File Config Window Help ix Cancel New gt STC Manager s2z0uv Y Open Ctrl 0 Save Ctrl 5 MNOPUIPE OL UOICWIOJU e19Ua5 99 UO INpoOI U After execution Calibration wp lp Is Completed appeas on a message b
16. e Activity e Set Range 1 Property Window Y axis shows transmittance or absorbance Set Min of 0 5 Max of Y 4 Minimum and maximum v Apply 1x Cancel e Set Activity Range 2 i Property Window Set interval in time for activity Start Activity 00 00 00 End Activity 00 01 00 v Apply 1x Cancel e Absorbance Transmittance 3 e cla dom ABS H Transmittance Select absorbance or transmittance e Activity Cursor Arrows Table 4 5 6 Click Activity will show interval in time and value will be shown on the right upper part as a table Click Cursor 5 leads displaying value of absorbance transmittance testing time and testing wavelength Arrows Table 6 leads before or next value in a table 32 5 Managing data value from Measurement F Measured data F click right side of mouse will lead to be shown as left Manipulate Show Only This Chart Change Title Delete e Manipulate Make Chart You can Manipulate the measured data Title 1 Graph Graph Plus Graph Constant Title 1 Name for New Chart Differentiate of graph v Apply x Cancel e Show only this chart You can see the only selected graph e Change title Change the name of selected data Changing name of selected data will lead some changes in title of table e Delete Delete measured data 33 onauiy aduis e ELEMENT o p
17. e Cursor at the wavelength to draw STC SUR NEW a la STC Save K amp T 4 gt Survey Scan 450 460 470 480 490 500 510 520 530 540 550 560 570 580 590 600 610 620 630 640 Wave 450 0 0 013 0 029 0 058 0 115 451 0 0 014 0 031 0 062 0 124 U d n U n l o A oo A ANANN o U id ed M IAN 4 Click to proceed to STC mode 5 At the concentration column meaningless values are written Please input the correct concentration values 29 U w uess AdAInS Z KAISTA EEINATEY A o U w om uess AdAInNS Z PEE Weeysi 0 30 ti Standard Curve Abs 0 016 C Asq 1 00000 w 15 20 2S 40 45 50 55 60 Seo oe Conc PPM Conc ABS ABS 1 ABS 2 ABS 3 ABS 4 ABS 5 10 0 159 20 0 319 50 0 795 100 1 587 QOOOd 8 amp RANAN Wavelength 526 No of Measurement 3 Type Linear 0 l Recalc v Apply x Cancel 6 After input the concentration click Make STC to draw STC Refer to Attentions while making amp modifying STC for other settings 7 Click Apply to save at STC manager 8 Import the STC at the ATC mode to measure the concentration of unknown samples 3 Simple Kinetic 1 Introduction Simple kinetic allows mutation or variation of samples in time interval at selected wavelength Test results will be shown as below table and graph 2 Display i OptizenView 4 2 mex File Config Window
18. e you through the installation of OptizenView 4 2 It is recommended that you close all other applications before starting Setup This will make it possible to update relevant system files without having to reboot your computer Click Next to continue To continue install program press Next When you like to stop installing press Cancle Process2 Select folder for software OptizenView r i OptizenView 4 2 Setup __ eau Choose Install Location Sun Choose the folder in which to install OptizenView 4 2 5 Setup will install OptizenView 4 2 in the following folder To install in a different folder dick Browse and select another folder Click Install to start the installation __ Browse Space required 32 6MB Space available 235 0GB Choose folder for software OptizenView For updating software for future reference and information suggest saving files that has been served by install wizard Suggested folder C WProgram Files OptizenView4 2 X Attention 4 2 means the version of the S W It may change without notice U feb M21 AUSZIIdQ dnyas EAE AEE E AEI AET Process3 6 Troubleshooting on process of installation U w s U fab Start to install On process of installation you might encounter to reboot computer OptizenView 4 2 Setup This incident of rebooting computer might happen after process 2 or on process 5 Proceed a
19. eels 4 Multiple Wave Scan 2 Display i OptizenView 4 2 1 Introduction File Config Window Help D C1e BH Z stc Manager 3220WV ver 4 tal This mode is to measure absorbances and calculate with preset equations at multi wavelength FF MUL New Method undo MeN e Terminology Name i escription O Wavelengths to be measured Factor List Factors to be used in equation TTE r3 STC List STCs to be used in equation C1 C2 C3 Calculated results o SO M ULNewMethod WAVE List 500 600 f An n 1 8 The ABS of No n wavelength at Wave List i iFactorList 1 2 F If Wave List is 500 600 5000nm 6000nm ci l i A1 ABS of 500 nm A2 AB S of 600 nm Sense J FT 1 Fn n 1 8 The Factor of No n kinds of a constant Ea If Factor List is 3 5 1 9 T onl z F1 3 Variables F2 5 SS J a 5 19 AM 10 14 2010 F3 1 9 3 Measure Sn n 1 8 The calculated result of An by No n STC i H List is 500 600 and f EmPeenene If STC List is STC_A STC_B 2 Input Name Wave List Factor List STC List C1 C2 C3 and do on B S1 the result that input ABS of 500 nm to STC_A S2 the result that input ABS of 600 nm to STC_B 3 Click and perform Base Line C 4 Click Measure D and you will get the results at table Measure UBDS aAeM NdINNW 7 saw CEA 0i me ere URIS BAM Ndw 7 FELayewCWey4i oi
20. en process alert to stop click Next to precede setup X Attention Window restart might repeat 2 3 times according to PC pecifications 7 Confirmation after installation Check installation of OptizenView After installation check existence of a directory as below has composed All process of setup has been successfully completed and new icon of OptizenView shall be on e In case of installing at the suggested folder your Display Completa all he process of instalation click Finish es ee ee Folder C Program Files OptizenView4 2 X Attention 4 2 means the version of the S W It may change without notice U e3 J Cae 04 U09 21Seg puy nuaw z EAA EAEE E AI belay AET 2 Menu And Basic Control 1 Toolbox OptizenView 4 2 File Config Window Help De amp amp G STC Manager i 3220uvver4 amp New Save Print STC Instrument Cell manager holder Open Saveas Basic Settings e New Select measuring mode li OptizenView 4 2 File Config Window Help Diele amp E srne sean ATC Survey Scan Kinetic Multi Wave STC Manager e Cell Holder Select cell type li OptizenView 4 2 10 File Config Window Help De Ow Er stem Semwves B 2 Multi Cell Single Cell Sipper Cell wad mak Micro Yolumn Cell Nana liter Cell Thermal Cell 2 File Management And Mode Selection e New File ti OptizenView 4 2
21. f auto detecting of peak ABS Slope 2 0000000949949E 03 ABS Distance 5 0 nm T Slope 0 100000001490116 T Distance 5 0 nm valley v Apply x Cancel e Absorbance Transmittance 3 Select absorbance or transmittance H Transmittance e Peak Valley Cursor Arrows 4 5 6 Click Peak Valley 4 to find Peak Valley In Peak Valley box click Cursor 5 will show absorbance transmittance and wavelength table arrows 6 can search before or next peak valley Managing 27 U w mi ueds AdAInS Z KAISTA EEINATEY A o U w om uess nans Z EIZA 0 28 5 Managing data value from Measurement F Measured data F click right side of mouse will lead to be Title 1 shown as left Manipulate Show Only This Chart Change Title Delete e Manipulate W Make Chart You can Manipulate the measured data Title 1 Graph Graph Plus Graph Constant Differentiate of graph Title 1 Name for New Chart v Apply x Cancel e Show only this chart You can see the only selected graph e Change title Change the name of selected data Changing name of selected data will lead some changes in title of table e Delete Delete measured data 6 Draw STC at SUR mode 1 Make the scanning spectrum of standard samples orderly 2 After measuring click Cursor 5 to check the ABS values of each wavelengths 3 Place th
22. lect absorbance or transmittance to show in Y axis of graph By selecting transform into either way for showing absorbance or H Transmittance e Single Cell Holder Mode transmittance Standard setting applied cell holders like Round cell holder Film cell holder and Long path cell holder Single cell holder shows as left Config C Cell Type Single Cell e Chart Color Setting Choose color of line figment in graph from color box 14 ibs Hed r ae oO jos c 2 fe Qo i i s feb fe 3 feb m fe uoljeunBijuog e 2 Options To set the basic conditions of the instrument select Config C Options in menu ti OptizenView 4 2 File Config Window Help 16 Select as above table box appears as below ti Options localhost AT Decimal 2 ABS Decimal 3 D2 Lamp Save NO Lamp Change 370 Initial Wave 600 of sT 0 01 of T 999 9 of ABS 0 5 of ABS 4 of Wave 190 of Wave 1100 g Initial Value v Apply wv Cancel e Device Name In a case of Stand Alone Version Write Localhost In a case of PC Version when user has personal computer connected for measuring with Optizen 3220UV write Default Optizen3220UV When using 2120 or 1412 input the name of PC connected to the instrument e 1T ABS Decimal Set decimal point in absorbance and transmittance e D2 Lamp Save If you
23. m Measurement eeeeeeesessssssessssssessessesesersesesseresesesessererrerereesenes 28 6 Draw STC at SUR mod ovrcerreeceeeeceeeeneeeeeeneeeceeneeeceseneueneeesneusereeensseeusneereueneeneneenseunensneunenenes 29 3 Simple Kinetic cc isnssssssssseseceseeeseeceeeeeeeeceeeeeeeeeeeeeeeeeeeteen 31 Wlnttcer fete mice ce cr ene eer et 31 2 Display PESEE EPEE Oto TAO A E TEREE A ES EESE E AA TEET E E EESE A EE Tae baa 31 3 IV ol at ccc 31 4 Graph Setting Ieeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeee eee REESE SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSIESE 32 5 Managing data value from Measurement or es 33 4 Multiple Wave Scan eeeeeeeeeeeesseeenieistiistittstitiniiststitinasesettinin 34 hitter ete ora ee eee Cee or eee Te ee 34 2 Display E E T 35 3 IV a ecco 35 4 Attentions while Multiple Wave Scam vvccccccccecessseseseeseseseseeeeeseeseeseeneceneneseseneeeestte af SRO Sete et ee ee re E E E ree 37 1 Faida olo 0 lend 0 a ALAA 37 2 Confirmation before MeaSUreMENt e remtomenncnacimeiatenrtanvmatrasnayn 37 5 1 Simple Absorbancelor Transmittance Measurement ore 39 5 2 Measuring Concentration using Standard Curve veers tenet 40 5 3 Measuring Absorbance Transmittance In Specific Wavelength Area vr 44 5 4 Measuring Absorbance Transmittance Timely scenes i Be ea carer gi te LMI se Gc aa dct elects aE detected tenet 48 6 Trobleshooting etettissrsiesseeissiisrerisstrseiisndisssnrttisintsratsinttsstesisasdieenisitiavenesensne iisa
24. o at system tray window e Print Select File F Print P and choose print form File Config Window Help to print out s2z0uv ver 4 New gt Open Ctrl 0 Save Ctrl 5 Save 4s Export to Excel Buljooysea qos 9 Pleyemeyywery4i ele men melee Chart Table Chart INQ UlIg pue ares o o EVEawitwecy4i ele mee meee Table 48 49 U w R r D a r gt Q w Ww ao O re s3 amga N Sjsoddns jes uysa 50 Part Ill Technical Supports 1 Technical Supports When you have any difficulties of installing Optizen 3220UV and OptizenView Please go through and review with this user s guide If troubleshooting does not help to solve troubles please contact your nearest distributor or producer Caution When contact for after service must be reported symptom of trouble Mecasys Co Ltd Postal code 305 150 10F 640 3 Bansuk dong Yuseong gu Daejeon Rep of KOREA Fax 82 42 485 7118 For technical service E mail maintenance mecasys co kr http english mecasys co kr
25. ox Save As Export to Excel Chart Table xi Chart Table Graph form uoieanyuon e KAEA ENEE AI be AET E TeeChart Print Preview SEE Orientation Portrait Landscape Reset Margins Iv View Margins 20 210 20 290 2 0 20 0 210 2 0 310 3 Ts i Was H Ussevevevsnsesenenansnsnssnsvanansnsenansnansnscasnansnanssensnansnanseassansnanssensnansnanasasuanansasensnanananaaanananansanenanenanen are 18 1 U feb INO Uld OL MIERO e19Ua5 E UO INpoOI U e Table Graph Table form Print SEE o KW g5 Print Cancel ATC Hew Method P hed Dat 2010 10 15 11 46 42 ATC New Method Preted Dae DIHS 11 46 42 a Perbmerh matba a Testlitomation v Ieas mg MODE i v Weve kigth i6oim iy Company v Standar Crue v Factor v Uiit pe Measure NANE ATC New Method WAVE 600 STC FACTOR _ _ e UNIT l iz aS TIME E Sample Wame Report Reporter Company Dept Comment Report Title Report Title ATC New Method Company Title Company Dept Title Comment Title Additional gt MEASURE gt Auto Zero Print g5 Print Cancel Previous page next page Starting print EY Zoom in out 20 Part OptizenView Usage 1 ABS T CONC and Standard Curve 1 Introduction This mode is to set Standard Curve at the specific Wavelength to find out Absorbance Tran
26. s Installing Please wait while OptizenView 4 2 is being installed below Skipped HTML Data Skipped SubModule X03 a Skipped SubModule X02 Skipped SubModule X01 Skipped SubModule X00 Skipped STC Data Skipped OptizenView exe Create folder C WUsers WEUNJEONG W AppData Roaming HMicrosoft t Windows t Create shortcut C Users WEUNJEONG TAppData WRoaming Microsoft Window Create shortcut C Users EUNJEONG Desktop WOptizenView Ink Extract Language txt 100 e Case 1 After process 2 On process 2 Restart message appears Press Yes to restart After rebooting Windows Process 3 starts automatically According to the process 3 m proceeds setup e Case 2 On process 5 if OptizenView Installer Information OptizenView is now on process of installation The installer must restart your system before configuration of Optizenview can continue Click Yes to restart now or Mo if you plan to restart later MAI AUSZIIdC ANAS EAR AEE E AEI AET M21 A USZIdQ dnyas EAO AEE E AEI AET Process4 Installation has been successfully completed _ d j On process 5 message box appears to restart as above F OptizenView 4 2 Setup e Click Yes to proceed restart Completing the OptizenView 4 2 pcan iain After rebooting Windows Process 5 will start continuously OptizenView 4 2 has been installed on your computer eee Wh
27. smittance and Concentration of samples 2 Display i OptizenView 4 2 File Config Window Help De lg amp Gr STC Manager 3220uv Bio ver 4 Q D C ATC New Method a la Standard Curve Sample Name Conc PPM Remarks Measure aoe e cece cece sccccccccssceesesscscceccesseeesescetey 4TC New Method i WAVE STC FACTOR He gt us 2 4 25 2010 10 14 3 Measure Multi cell holder Mode 1 Select cell number A 2 Input wavelength factor unit and so on to measuring condition C When taking concentration by Standard Curve Click and select standard curve frame B 3 Select Auto Zero D 4 Select Measure E and you can see the measured data on table 21 I Hed abesp mal uaziido BAIND pJepue s pue QDNOOD 1L SAV l SAIND pJepue s pue DNOID 1 SAV FAAEA eo E ESS 4 Graph Setting Set Min Max of Y axis 1 A ATC New Method You can set min max value of Property Window Mlin of 0 5 Max of Y 4 v Apply x Cancel 5 STC open or Select Open STC Manager and select one STC Standard Curve XxX Usage of STC Manager Refer 23page 22 Y axis ABS of Standard Curve 6 Usage of STC Manager This mode is to make modify delete find and open Standard Curve STC STCs saved in STC Manager can be shared by several ATC modes To transfer STC to other PCs click Expor
28. t and save STC as a separate file And on the contrary you can open STC file by selecting Import i STC Manager BASIC AY import Export New STC 1 CO New STC 1 0 I I I I 0 2 4 6 8 10 12 14 16 18 20 Conc PPM 600 nm RSQ 1 y 0 02 C W New STC gt Modify x Delete Select e Import A Open STC file that is exported from other instruments e Export B Save STC as a separate file for transfer or backup purposes e New STC C Modify D e Delete E Delete selected STC e Select F 23 SAIND pJepue s pue DNOID 1 SAV PLUS NUE eo ESL SAIND pJepue s pue DNOID 1 SAV PLUS NEY eo i ESS As selecting New STC C or Modify D you can see the window as below Standard Curye not defined Standard Curve ABS ABS 1 ABS 2 ABS 4 ABS 5 v Apply x Cancel 1 Input name of this STC B 2 Input wavelength C 3 Input measuring times D 4 Set unit E 5 Input the concentration of the standard sample A 6 Press AutoZero F 7 Press Measure to get the ABS value G 8 After measuring click Type H and Recalc l to get the proper STC 9 Click Apply to save at STC manager 24 7 Attentions while making amp modifying STC a No of Measurement means the number of measuring times of each standard samples b After measuring if you discard the specific result click square left side of A and
29. tteaatuasddisaemedeiuede baer hisereeendeeenies 1 Cell Type es ic eh E E eS ab es pc hilo er tc cs ete os a E E E aetna 2 Options A E na E E E E E E E E E E E E E ues 3 Calibration Only applied in OPTIZEN 3220UV pid E T E A E E ds vengeance Print 10 ieeeeeeee eee RRS OEE CECOO OS CCCOOOOCOOOOOSOOOOOOSOCOOOOOOOCOOOOOOOOCOOOOOOOOOOOSOOCOOOOOOOOOOOOOOOOOOSS D Part OptizenView Usage ABSIT CONC and Standard Curve lt ctieremneraten camnnriimwienuinnmnnennninamet il introduction eene ee ee ee 2 Display ores ieee eens 3j WGA Ci eneses R T Ea 4 Graph Setting eeeeeeeeeerteetttterrttterttttttstttttstssttttetttssntttttttesnntettttestt tent JEI RRtaaS x caaS CESS SESS TOSS SESSS TESS SOSSSSESSSSOSS SESS SOSSSSESSSSOSSSSOSSSCOSSSSOSSSSOSSSSOSSSSOSSSSESSSCOSSSSOSSSSOSESSOSE 6 Usage of STC Manager vrei eisai 7 Attentions while making amp Modifying STC eeeeeeeeeeeeeserteeeetrete 8 Calculating value R in a regression line eee ee meee ewe ewe ee eee eee ee eee eee eee eee eee eee eee eee eee eee eee eee eeeees 2 Survey LOY r a eeeeeesteertstserrereseresreseserrsseseserreseseresseserenresesesrsresereseeserereestserisraeeserrareserereareseresresesene 26 Weintects cule et eee ee ee eee ee ee J 2 Display TE E E E E E A E 26 3 Measure eeeessssssssssssstssesesesessererresererresererteserestartnereasreesessrsesesrreeresrasrseseaseseseaeeserenrasereseeserereasene 26 4 Graph Setting E E E E T E E E ake E E 27 5 Managing data value fro
30. use visible light source area mostly you can save power and lengthen lifespan of D2 lamp by turning off D2 lamp However frequent switching power of D2 lamp might cause shortening lifespan of D2 lamp Attention D2 lamp is one of expensive part in your instrument e Lamp Change You can set wavelength change point in D2 lamp and W lamp wavelength range 340nm 410nm e T ABS Max Min Set minimum maximum of measured value in absorbance and transmittance e Wavelength Max Min Select minimum maximum wavelength range wavelength range 190nm 1100nm For 1412 range is from 340nm to 1100 nm 3 Calibration Only applied in OPTIZEN 3220UV To calibrate OPTIZEN 3220UV select Config C Advanced Setting Calibration OptizenView 4 2 This is to calibrate wavelength and cell File Config Window Help holder positioning 2 Cell Type ger Sez0lBio vert After manufacturing on process of quality Q Options Q Change Display Mode j 5 Advanced Setting gt Calibratoion S Select Other Device SUR New Method suene control we Mecasys have done all calibration for correction in wavelength and cell positioning You don t need to calibrate Comport without special reasons Please be aware that calibration is usually done under manufacturer s strict guideline Calibration Ed Calibration Before After WP_A 4125 WP_A 4125 WP_B 4091 wee 4091 WP_C 4125 WP
31. uto Zero dAINY psepueys Buisn uoljesjuadu0g Hbulunseay z o EAZA EAEAIEYA ele JAIN psepueys Buisn uoljesjuadu0g bulunseay z o Eyam wecy4bele While Auto Zero is executing Executing Auto Zero appears on Message Box G after execution Ready appears 42 43 5 3 Measuring Absorbance Transmittance In Specific Wavelength Area 2 Input select Name of Test Start wavelength finish point wavelength Step and Scan Speed C OptizenView 4 2 File Config Window Help G Measure a oe wal NAME SUR New Method 2 5 la v i ae vo B Me START WAVE 500 i Bi o mi Vi Hi ar EEE TE ENDWAVE 600 N Ta STEP 5 e a 3 G P E E D NORMAL 3 First and before measuring samples click Base Line D for setting blank g earns Se AN 7 gt MEASURE gt I Auto Zero 6 AME SUR New Method f START WAVE 500 ij a f naen EEFIN While execution Executing Base Line Collection appears on Message Box F and after poe e execution Ready appears E 4 Select Cell Number CELL 1 No 1 B Wave i Welcome a IK gt 4 gt a vi a z Eas am 10 142010 B a 5 3 E ra E 1 Locate Mouse point New Open A left side and mode selections will appear as shown 5 After Cell selection Click Measure E to start measurement Fie Config Window Help o f m De l amp amp Be STC Manager 322008 b MEASURE I Auto Zero
Download Pdf Manuals
Related Search