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223 Sample Changer User's Guide

1. e Check for obstructions Cycle power to the sample changer e Check for obstructions Cycle power to the sample changer e Check for obstructions Check if the sensor disk is bent Then contact the Gilson Customer Service Department Check if the sensor disk is bent Then contact the Gilson Customer Service Department Check if the sensor disk is bent Then contact the Gilson Customer Service Department Correct the error in the program controlling the sample changer Correct the error in the program controlling the sample changer Send the immediate Q command using the GSIOC Utility Program to read the travel range Correct the error in the program controlling the sample changer Run SET_223 EXE or the 223 Setup Utility Adjust XY tab program to correct the problem If the problem persists contact the Gilson Customer Service Department Troubleshooting 30 31 Y offset out of range Z offset out of range 5 Run SET_223 EXE or the 223 Setup Utility Adjust XY tab program to correct the problem If the problem persists contact the Gilson Customer Service Department Run SET_223 EXE or the 223 Setup Utility Adjust XY tab program to correct the problem If the problem persists contact the Gilson Customer Service Department 5 3 Troubleshooting Mechanical Problems 5 4 Mechanical Problems Probe not finding tube center Probe may be bent Straighten or replace the probe The samp
2. Error Handling Arm Travel XYZ Coordinates Contacts Homing Adjust XY Liquid Detector Z Arm Following is a description of each of the tabs Operation About Fe 223 Setup Utility The About tab allows you to indicate the unit ID of the instrument being configured so the 223 Setup Utility can communicate with that instrument This tab also lists software version and copyright information for the 223 Setup Utility oo suondo uonjeinbyuoy Buryoeyy 3 7 Operation Checking Configuration Options 3 8 Adjust XY Fe 223 Setup Utility Jof x Contacts __8YZ Coordinates Error Handling About Liquid Detector ZA m AmTravel Homing Current XY Offsets gt Manual Nudge Default Xx Offset 1 8 in millimeters Default Y Offset 2 2 in millimeters eS H ag es aes Y 0 0 mm Move to Test Target 320 3 y s am Home OK Cancel Help The Adjust XY tab allows you to test whether the instrument is properly adjusted and to make minor adjustments to the X and Y axis offsets if needed You may need to use the options under this tab if the probe is not accessing the transfer port filler port or the vessels in the installed racks The X offset and Y offset text boxes display the current offsets stored in the instrument s memory To determine if the probe s need s to be adjusted in the X or Y direction 1 Select a test point 2 Click on Move When the
3. s surface To install the liquid level detector cable 1 Remove the probe retaining screw from the probe holder 2 Slide the ring contact of the liquid level detector cable into the slot provided on the probe holder 3 Secure the probe retaining screw 4 Plug the end of the cable into the coaxial socket probe coaxial socket To disconnect the cable from the front face of the sample changer you need to pull the collar on the connector towards you before you can detach the connector Installation Diverting Valve To install the diverting valve 1 Turn off power to the sample changer and if installed its dilutor If installed disconnect the dilutor s transfer tubing from the probe Locate the larger tubing cable clip supplied with the diverting valve Attach the clip to the left of the vertical arm in the center of the horizontal arm Locate the smaller tubing cable clip Attach the clip to the back of the vertical arm the side that faces the rear panel of the sample changer position the clip approximately 1 3 of the distance from the top of the vertical arm to its bottom Remove the protective covers from the bolts located on the left side of the vertical arm Attach the valve to the left side of the vertical arm by inserting the mounting bolts through the two holes on the valve s mounting bracket Secure the valve using the two self locking nuts When placing the nuts onto
4. Outside the United States contact your Gilson representative for assistance Specific contact information can be found on the Gilson website at www gilson com To help us serve you quickly and efficiently please refer to Before calling us on page 5 8 sdIAIOGI9WIO SND 1 3 Introduction Technical Specifications 1 4 Technical Specifications The following information is subject to change without notice Warning Changes or modifications to the sample changer not expressly approved by Gilson could void the factory authorized warranty The sample changer has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC commercial environment The sample changer generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications Operation of the sample changer in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at the user s own expense Shielded cables must be used with the sample changer to ensure compliance with the Class A FCC limits Introduction Technical Specification Definition Arm speed gt 250 mm sec gt 9 85 in sec in X Y dimension Z dimension has five programmable speeds for upward and downward movement Index Speed mm sec 1 19 9
5. Plastic locking collars to connect drain tubing to rinse station drain package of 6 Tubing for flow through rinsing well 3 16 ID x 5 16 OD per foot Tygon tubing for rinse station drain 5 32 ID x 7 32 OD per foot Tygon tubing for polypropylene tray drain 5 16 ID x 7 16 OD per foot Isoversinic drain tubing for rinse station drain 7 mm ID per foot Rinse station tubing Isoversinic 4 5 mm ID 6 mm OD per foot For rinse stations with locking collar Appendix Control Options 210677352 2106171 270230 36083121 36083122 36083123 Safety Shield 1907175 Accessories 2749767 49944107 49942107 2954709 2954714 2954833 190711 1907111 030722 gt 735 Sampler Software for NEBULA Series products and XL samplers The Windows based relational database designed software controls the 223 Sample Changer 215 Nebula Series liquid handlers and Autoinjectors 234 and 235 Autoinjectors 231 XL 232 XL and 233 XL Sampling Injectors ASPEC XL XLi and XL4 Solid Phase Extraction Systems the 221 XL and 222 XL Liquid Handlers via a graphical user interface the Quad Z 215 and the SPE 215 System Requires Microsoft Windows 98 or NT and serial cable Computer requirements are Pentium 600 MHz 64 Mb RAM 50Mb hard disk space available and CD ROM minimum configuration 706 GSIOC Programmer s Tool Kit for MS DOS based systems XL Keypad Serial Cable 25 pin 25 pin Serial Cable 9 pin 25 pin most comm
6. Holder To install the probe holder slide it into place on the probe holder bracket and secure with the supplied screw Installing Probe To install the probe 1 2 Slide the probe into the tubing fitting Connect the transfer tubing from the dilutor if installed to the tubing fitting Slide the probe assembly into the probe holder on the vertical arm making sure that the probe is centered over the probe guide Secure the probe by tightening the probe retaining screw uo ejjesu 3qold 2 19 Installation Rinse Station and Support Bar Installation 2 20 rinse station optional wee i rinse station T t of Si port bar rinsing well level sensing rinsing well locking collar tubing Rinse Station and Support Bar Installation The support bar attaches to the front face of the sample changer Its rear contains mounting holes for rinse stations and its top contains mounting holes for transfer ports and filler ports Note If you ordered rack holder reference 1907142 or rack holder reference 1907143 the support bar is part of the rack holder assembly Attach any rinse stations transfer ports or filler ports to the rack holder s support bar 1 Attach the rinse station s to the support bar If using only one rinse station the default position for the rinse station is the left side of the support bar when the bar is attached to the sample changer From left to right th
7. To use Code 30P series racks you must install rack holder reference 1907142 Installing rack holder reference 1907143 or reference 1907142 is described on the next page To use microplates you must install microplate holder reference 1907151 See page 2 25 uoleyye suy yory 2 23 Installation Accessory Installation 2 24 Accessory Installation Rack Holder Installing a rack holder enables you to position a single rack sideways within the sample changer s work area A Code 0 7 8 or 9 rack requires rack holder reference 1907143 A Code 30P series rack requires rack holder reference 1907142 Note You can also install one Code 30 series rack on rack holder reference 1907142 Before installing the rack holder you may need to remove the support bar from the sample changer Then remove any rinse stations filler port and transfer ports from the support bar and place each one in the corresponding location on the rack holder s support bar Connect the rack holder s support bar to the upper position of the front standoffs of the sample changer in the same position that the previous support bar was located Place the rack onto the rack holder s arms A Code 20 or 30 series rack can be placed on the tray in the far right position Note If the 85 mm rinsing well is installed on the rinse station install the tray in the lower position rack holder s support bar front standoff tray additional rac
8. contains two 2 0A fuses for a 220 240 voltage selection Fuse installation for Fuse installation for 100 120 voltage 220 240 voltage 5 Remove the old fuse s and insert the new fuse s 6 Insert the fuse drawer into its receptacle in the sample changer 4 5 Maintenance Checking Position Alignment 4 6 Checking Position Alignment Fe 223 Setup Utility of x Contacts XYZ Coordinate Error Handling About Liquid Detector AimTravel Homing Current XY Offsets M Manual Nudge Default x Offset 1 8 in millimeters 4 Default Y Offset 2 2 in millimeters Ze Ea gt z 4 Y 0 0 mm gt Move to Test Target aai Y pis aa Home OK Cancel Help The 223 Setup Utility Adjust XY tab described in Section 3 allows you to test whether the instrument is properly adjusted and to make minor adjustments to the X and Y axis offsets if needed You may need to use the options under this tab if the probe is not accessing the transfer port filler port or the vessels in the installed racks The X offset and Y offset text boxes display the current offsets stored in the instrument s memory To determine if the probe s need s to be adjusted in the X or Y direction 1 Select a test point 2 Click on Move When the probe reaches the target site the software relaxes the Z Arm so you can manually raise and lower the probe for visual alignment co
9. is set to 10 There is no need to change this number unless it is the same as that assigned to another GSIOC device that is also connected along the GSIOC To change the unit ID 1 Gently insert a small flat blade screwdriver into the SW1 selector on the rear panel and turn it 2 Align the white dot with one of the indicated numbers The unit ID is 10 plus the selected number Installation Baud Rate As a default the baud rate selector is set to 0 for identifying a baud rate of 19200 for keypad or computer control or for identifying an external clock for GSIOC control The baud rate for the Keypad Controller is 19200 so do not modify the SW2 selection if a keypad is connected to the sample changer If your computer s baud rate is 9600 change the setting for the SW2 selector to 1 or 3 1 Gently insert a small flat blade screwdriver into the SW2 selector on the rear panel and turn it 2 Align the white dot with 1 or 3 If the GSIOC is the master device and the GSIOC source does not have a clock for example a Macintosh computer change the setting to 2 or 3 using the procedure above 2 33 Operation 3 The 223 Sample Changer is controlled as follows e Via a method running ona personal computer You create the method using 735 Sampler Software or 709 Sampler Manager Software All are ordered separately See Appendix A Replacement Parts and Accessories for reference numbers e Via an executable fil
10. panel away from the sample changer 6 Turn over the control panel so you can see its components 7 Locate the termination switch The switch is below the GSIOC connector Refer to the following diagram Input Output SW1 and SW2 GSIOC Keypad RS 232 connector switches connector connector connector Pin 1 marker i a Termination switch CPU board 8 Move the termination switch to the break active Termination position Refer to the following diagram eae Pin 1 __ i marker 9 Reinstall the control panel and tighten its screws M Break 10 Reinstall the rear panel and tighten its screws paSaNE ann 11 Connect the power cord and turn on power to the sample changer Break active 2 11 JOISS Installation Input Output Contacts 2 12 Input Output Contacts You can use the input and output contacts found on the rear panel of the sample changer to control peripheral devices Refer to the diagram on page 2 3 for the location of the input output ports Inputs The sample changer has connections for four inputs All of the inputs are paired and each of the pairs includes a GROUND reference 4 The contact input pairs are labeled A B C and D A contact is connected if it has a short across the input or is held low by a TTL output or other device Never connect voltages higher
11. port enables you to link a GSIOC compatible module to the sample changer and control both devices via a program executed on the computer or Keypad Controller 1 Locate the accessory package with the GSIOC cable as 30 gt 9 pin female 4 E acai pin female ahha ff connector connect GSIOC port of K gt LJ to sample changer v GSIOC module W U 9 pin male connector connect to GSIOC cable for linking additional GSIOC modules 2 Connect the female connector located individually at one end of the cable into the GSIOC port of the sample changer Tighten the retaining screws 3 Connect the other female connector located on the same end as the male connector to the GSIOC module Tighten the retaining screws 4 Ifyou are connecting another GSIOC module use the male connector to join another GSIOC cable and make the necessary connection to the next GSIOC module The following diagram shows the cabling connections between a computer sample changer and GSIOC module RS 232 sample changer cable Computer JL GSIOC cable GSIOC module JOISS Installation GSIOC 2 8 computer RS 232 cable If the GSIOC is the master device the GSIOC port also enables you to link the sample changer and other GSIOC compatible modules I
12. the probe to several locations Warning Remove the probe before running this test oo suondo uoneinbyuoy Bulyoeyy 3 13 Operation Checking Configuration Options 3 14 XYZ Coordinates Fe 223 Setup Utility of x Liquid Detector Adiustxy Zam AimTravel Homing Contacts V2 Coordinates Eror Handing About Move the probe to the desired position after disabling motors Current position in tenths of millimeters oo00 yy 0000 Home Disable Motors Help Use this tab to identify the X Y Z coordinates of locations on the sample changer s work area and Z coordinate of the probe s tip First click Disable Motors Then manually move the horizontal and vertical arms to a location on the sample changer s work area Finally manually lower or raise the probe to the appropriate height The 223 Setup Utility displays the X Y and Z coordinates vertical arm probe Z axis horizontal arm Operation Homing Fe 223 Setup Utility This tab allows you to choose whether the instrument should home when powered up click Unit homes in XYZ on power up or whether the motors in the arm should be disabled when the instrument powers up click Unit stays relaxed on power up oo suondo uoneinbyuoy Bulyoeyy 3 15 Maintenance 4 To obtain optimum performance and maximum life from the sample changer keep it well maintained This sect
13. 160 5 x 1 5 x 1 1 mm ID Requires probe holder guide kit reference 19061040 Tapered tip probe not septum piercing 160 5 x 1 5 x 1 1 mm ID Requires probe holder guide kit reference 19061040 Side entry tip probe septum piercing 160 5 x 1 5 x 1 1 mm ID Requires probe holder guide kit reference 19061040 Beveled edge probe for use with transfer ports not septum piercing 160 5 x 1 5 x 1 1 mm Requires probe holder guide kit reference 19061040 Beveled edge probe septum piercing 162 x 0 7 x 0 42 mm Requires probe sleeve reference 27067236 and probe holder guide kit reference 19061040 Probe sleeve for septum piercing probe 133 x 2 x 0 8 mm reference 27067235 Requires probe holder guide kit reference 19061040 Beveled edge probe not septum piercing 162 x 0 7 x 0 42 mm Requires sleeve reference 27067232 and probe holder guide kit reference 19061040 Probe sleeve for non septum piercing probe reference 27067231 Requires probe holder guide kit reference 19061040 Flat tipped probe for drop transfer 165 x 0 7 x 0 4 mm Requires probe sleeve reference 27067277 Requires probe holder guide kit reference 19061040 Probe sleeve for flat tipped probe 155 mm without V cut at top Requires probe holder guide kit reference 19061040 Grooved septum piercing probe sleeve not required 162 x 0 7 x 0 4 mm ID Requires 1 6 mm probe guide insert reference 27067239 Titanium bevele
14. 4 1 to connect 0 to disconnect X for no change p Optional Auxiliary power 1 to connect 0 to disconnect X for no change Appendix jc ttt Lx NAF Smm Pulses an output contact c Number of the output contact 1 4 ttt Duration of the pulse in tenths of seconds default is 1 Sets liquid level sensing threshold frequency based on current frequency and data at NV RAM frequency threshold field spuewwop J0IS9 x H for high frequency setting or L for low frequency Reads X Y Z motor status Returns xyz For each motor status you see U for unpowered P for powered R for running or E for error Reads the actual frequency of liquid level detector oscillator Returns ffff which is frequency in Hz Reads the liquid level detector output Returns Iffff where I A for air or L for liquid ffff Current sensitivity threshold frequency in Hz Sets the liquid level sensing threshold frequency ffff in Hz Reads the XYZ position in tenths of millimeters based on the internal encoder wheel Reads the Z travel range Returns min max where min Lowest position in tenths of millimeters max Highest position in tenths of millimeters Reads the command in the synchronization buffer Returns if buffer is empty Sends a synchronized buffered command mm that is executed when the sample changer is quiescent Send ing a command can
15. Default for me level seeking 3 61 8 4 126 9 Default for normal travel 5 247 3 Contact control Four inputs accepts contact closure TTL or open collector signals four relay outputs and one switched 12V DC output 500 mA max Environmental conditions Indoor use Altitude Up to 2000 m Temperature range 5 40 C Air pressure 75 105 kPa Pollution degree 1 or 2 in accordance to IEC 66 Humidity Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Front panel One yellow LED Horizontal motion strength X 2 kg 5 lbs Y 1 5 kg 3 Ibs suoiesijioads jesruy2 L 1 5 Introduction Technical Specifications 1 6 Liquid level sensing Capacitive Manufacturing standards Safety certification UL 1262 CSA C22 2 No 151 IEC 1010 1 EMC EMI certification EN 61326 1997 EN 61000 3 2 1995 EN 61000 3 3 1995 FCC Part 15 Class A Physical space requirement w x d x h 53 5 x 43 7 x 60 0 cm 21 1 x 17 2 x 24 0 in Maximum height Z arm height is adjustable to accommodate vessel heights between 1 and 150 mm dependent on installed Z arm Power requirements Frequency 50 to 60 Hz Voltage 90 120 V or 220 240V mains voltage fluctuations not to exceed 10 of the nominal voltage Current rating One 2 0A fuse for 90 120V or two 2 0A fuses for 220 240V Probe positioning A
16. Gilson 223 Sample Changer User s Guide LT1931 2001 Gilson Inc All rights reserved October 2001 Table of Contents Declaration of Conformity 1 Introduction Unpacking Standard Equipment Accessories Customer Service Technical Specifications 2 Installation Changing Termination Switch Input Output Contacts Items You Need to Make Connections Making Connections Power Cord Arm Locking Screw Removal Vertical Arm Installation Probe Installation Installing Probe Guide Installing Probe Holder Installing Probe Rinse Station and Support Bar Installation Tray Installation Lower Position Upper Position Tray Removal Rack Installation Accessory Installation Rack Holder Microplate Holder Tubing Cable Support Rod Transfer Port Filler Port Liquid Level Detector Diverting Valve Unit ID and Baud Rate Selection 3 Operation Start Up Running Programs Program for Windows NT users Programs for Gilson Keypad Users Running Programs from the Keypad Checking Configuration Options Adjust XY Arm Travel Liquid Detector Contacts Error Handling XYZ Coordinates 4 Maintenance Cleaning the Sample Changer Replacing Parts Replacing the Probe Replacing a Fuse Checking Position Alignment Transporting the Sample Changer 5 Troubleshooting Error Messages Mechanical Problems Electrical Problems Liquid Level Detector Problems Repair and Return Policies AppendixA Replacement Parts and Accessories Vertical Arms Doub
17. ID Cut to required length according to probe length and sample vial gt sallossedoy puke Sed zuU wW JLjd y A 3 Appendix Replacement Parts and Accessories A 4 Probe Holder Guide Kits 19061040 19061041 Transfer Tubing 2954583 2954671 29425066 2954672 49943392 49942392 49948392 F1410153 27072001 23074002 Probe holder guide kit includes probe holder guide and probe connecting nut reference 27072001 for 1 5mm outer diameter probes Probe holder guide kit includes probe holder guide and probe connecting nut reference 23074002 for Teflon probes 1 1 ml coiled FEP tubing 2200 x 1 6 x 0 8 mm ID 3 ml coiled FEP tubing 1 5 mm ID 5 ml coiled FEP tubing 1 5 mm ID 10 ml coiled FEP tubing 1 5 mm ID 200 ul FEP transfer tubing 1000 x 1 6 x 0 5 mm 440 ul FEP transfer tubing 1000 x 1 6 x 0 8 mm 2800 ul FEP transfer tubing 1000 x 3 x 2 mm Coupling for 1 4 28 fitting PVDF Probe connecting nut for 1 5 mm probe connects probe and transfer tubing Probe connecting nut for Teflon probe connects probe and transfer tubing Rinse Stations and Accessories 2707251L 2707261L 2707263 2707253 2707252 29501017 470331206 470321803 470343706 13420034 4701177592 Rinse station with 85 mm rinsing well and locking collar Rinse station with 45 mm rinsing well and locking collar Polypropylene rinsing well 45 mm Polypropylene rinsing well 85 mm Polypropylene rinsing well flow through
18. ate of all four contacts and the time since the last buffered 9 command was issued are stored in the event FIFO Clears the contact event FIFO and resets the contact input event timer spuewiwod J0IS9 C 3 GSIOC Commands Appendix a I b I c I e I e B Exyz B H B I I J I Jeccc p B C4 Returns X travel length in Kilometers Returns Y travel length in Kilometers Returns Z travel length in Kilometers Reads the current error number Returns n which identifies the error number see page 5 2 for listing of errors If no error has occurred returns 0 Clears error number Sets X Y and Z motor status x 0 for disable or 1 for enable X motor y 0 for disable or 1 for enable Y motor z O for disable or 1 for enable Z motor For example the following command disables the motors E000 Moves probe to home position Reads status of input contacts Returns cccc where ccce Status of input contacts A B C D 1 if the contact is closed shorted 0 if open Reads status of output contacts and 12 V external auxiliary power Returns ccccp where ccce Status of output contacts 1 2 3 and 4 1 if the output is connected 0 if disconnected p Status of 12 V external auxiliary power 1 if connected 0 if disconnected Auxiliary power is off when sampler changer is turned on Sets status of output contacts and 12 V external auxiliary power cccc Output contacts 1 2 3 and
19. ccuracy performance 1 0 mm in the XYZ dimensions Repeatability 0 25 mm in the XYZ dimensions Probe rinse Probe rinsing occurs through a dedicated rinse station for rinsing the inside and outside of the probe Number of racks Up to four Code 20 or Code 30 series racks Sample changer type XYZ with stationary rack design Introduction Software control Computer control via RS 232 or GSIOC and Gilson control software Local control via Gilson Keypad Controller Vertical punch strength 1 kg 2 Ibs Weight 18 6 kg 41 lbs suoiesiioeds jesiuy2 L 1 7 Installation This section describes how to set up the 223 Sample Changer The diagram below shows a possible configuration of the sample changer vertical arm Z axis horizontal arm rinse station mI status light probe support bar 2 1 Installation Rear Panel 2 2 Rear Panel The following pages describe how to make rear panel connections Before operating the sample changer you must connect it to one of the following e toacomputer s RS 232 port the computer is the master device e toa Keypad Controller the Keypad Controller is the master device e to the Gilson Serial Input Output Channel GSIOC the GSIOC is the master device In this configuration a Gilson 506C System Interface is connected to a computer via an RS 232 cable The sampl
20. d edge probe septum piercing 162 x 0 7 x 0 42 mm Requires probe sleeve reference 27067236 Requires probe holder guide kit reference 19061040 Probe sleeve for septum piercing probe reference 27067237 Appendix Teflon 27067218 Double Probe Kit 27167215 Teflon coated probe for non metal applications 157 5 x 0 9 mm ID 22 GA Requires probe holder guide kit 19061041 Double probe kit allows different simultaneous or successive liquid handling operations in same test tube Requires 3 way valve Includes flat tipped probe 181 5 x 1 5 x 1 1 mm ID triangular flat tipped probe 169 5 x 2 5 x 1 1 mm ID Teflon connector probe holder amp probe guide Probes for 56 mm Vertical Arm Stainless Steel 27067131 27067132 27067113 Titanium 27067133 27067132 Beveled edge probe septum piercing 95 x 0 7 x 0 42 mm Requires probe sleeve reference 27067132 and probe holder guide kit reference 19061040 Probe sleeve for septum piercing probe reference 27067131 Constricting tip probe 93 5 x 1 5 x 1 1 mm Requires probe holder guide kit reference 19061040 Beveled edge probe septum piercing 95 x 0 7 x 0 42 mm Requires probe sleeve reference 27067132 and probe holder guide kit reference 19061040 Probe sleeve for septum piercing probe reference 27067133 Adjustable Probe Sleeve 27067267 Adjustable probe sleeve for septum piercing probes stainless steel 160 x 1 2 x 0 8 mm
21. d except for auxiliary parts such as the vertical arm probe tray racks etc Keep the original container and packing assembly in case the sample changer must be returned Standard Equipment Once the sample changer and accessories are unpacked you should have the following e 223 Sample Changer and User s Guide e Vertical arm e Accessory package with terminal block connectors fuse drawers fuses power cords serial cable and 3 feet of 7 mm ID Tygon tubing e 223 Utility Programs CD ROM Accessories Based on your requirements you also received additional accessories such as probe holder guide kit tray tray spacers support bar rinse station rack holder racks liquid level detection cable diverting valve filler port transfer port kit tubing cable support rod GSIOC cable RS 232 cable Keypad Contoller syringe pump or peristaltic pump If necessary refer to Appendix A for reference numbers Introduction Customer Service Gilson Inc and its worldwide network of authorized representatives provide you with the following types of assistance sales technical applications and instrument repair If you need assistance and are in the United States please contact your regional Gilson representative or call the Gilson Customer Service Department at 800 445 7661 or 608 836 1551 You can also contact the Gilson Customer Service Department via its electronic mail e mail address service gilson com
22. d to enter in place of the italicized parameter Also note that if a parameter is optional it appears within brackets I Immediate B Buffered Description Returns the character string 223Vx yz where x y and z identify the software version for the sample changer Returns a and resets the sample changer to its power up state Reads non volatile memory NV RAM at current address Returns AA xxxx where AA Value is the address 0 39 xxxx Data at the address Current address is incremented after response is returned Appendix AA xxxx n B Sets the value at NV RAM address where AA Value is the address 0 39 see list below xxxx Optional Data at the address Sets test mode Indicate one of the following for n 1 Performs XYZ test Must be run without probe from the home position 9 Resets NV RAM and initializes to defaults Reads contact input event FIFO If the queue is empty 000000 is returned If the queue is not empty returns Xtttttt where X State of the four contact inputs 1 for closed 0 for open See table below tttttt Time in 10 ms units since the last buffered 9 command if X then A B C D 0 0 0 0 A 1 0 0 0 B 0 1 0 0 C 1 1 0 0 D 0 0 1 0 E 1 0 1 0 F 0 1 1 0 G 1 1 1 0 H 0 0 0 1 I 1 0 0 1 J 0 1 0 1 K 1 1 0 1 L 0 0 1 1 M 1 0 1 1 O 0 1 1 1 P 1 1 1 1 Whenever the status of a contact input changes the st
23. e changer and other GSIOC compatible modules are connected to the system interface via GSIOC cable s You also must install the fuse s and connect the sample changer s power cord to an outlet Contact connections are optional Installation INPUT Lo 4 a aaa swi sw2 TT SoNcDcDaARANe Input Output I O contact ports Unit ID selector Baud rate selector Gilson Serial Input Output Channel GSIOC port Keypad port RS 232 port Power receptacle Power switch Fuse drawer joueg 1e9y Fuses Installation 2 4 Fuse installation for 100 120 voltage Fuse installation for 220 240 voltage Fuses You receive the sample changer without any fuses installed 1 Locate the accessory package containing the fuse drawer appropriate for your line voltage Discard the other fuse drawer Locate the accessory package containing the 2 0 amp fuses Install the fuse s into the fuse drawer The fuse drawer for 100 120V accepts one fuse The fuse drawer for 220 240V accepts two fuses Insert the fuse drawer into its receptacle in the sample changer See rear panel diagram on page 2 3 Installation 2 RS 232 c e Su The RS 232 port is used to transfer information between the sample changer and a compu
24. e list of operating procedures and conditions you were using when the problem arose e list of other devices connected to the workstation and a description of those connections e list of other electrical connections in the room Warranty repair Units covered under warranty will be repaired and returned to you at no charge If you have any questions about applicability please contact Gilson or your authorized representative Non warranty repair For out of warranty repairs contact your local Gilson representative or the Gilson Customer Service Department A Customer Service representative will discuss service options with you and can assist in making arrangements to return the equipment if necessary Troubleshooting Rebuilt exchange For some units rebuilt exchange components are available Contact Gilson for details Return procedure In the United States contact the Gilson Customer Service Department to obtain authorization before returning any Gilson equipment To return a piece of equipment Carefully pack the unit to prevent damage in transit Check with Gilson regarding proper method of shipment No responsibility is assumed by Gilson for damage caused by improperly packaged instruments Indicate the authorization on the carton and on the packing slip Always insure for the replacement value of the unit Include a description of symptoms your name address phone number and purchase order to cover repair cos
25. e ports in a rinse station are the rinsing well level sensing rinsing well and drain To attach tubing to the drain locate the Tygon drain tubing reference 470321803 or Isoversinic drain tubing reference 13420034 Remove the locking collar from the drain fitting Slide the tubing through the locking collar Attach the tubing to the drain fitting Then screw the collar onto the drain fitting Place the other end of the tubing in a drain receptacle located lower than the tray If you ordered a flow through rinsing well remove the rinsing well and attach the flow through rinsing well in its place Then attach tubing reference 470331206 to the flow through rinsing well and connect the other end to a liquid source 2 Attach the support bar to the front of the sample changer using the knurled screws Installation Tray Installation The tray positions the racks and any accessories that fit onto the bed of the sample changer It also contains liquid spills such as those caused by overflowing vessels The tray can be installed in an upper or lower position on the sample changer s bed depending on the size of tubes being used Lower Position If you are using tubes that are more than 100 mm in height install the tray in the lower position on the tray mounting brackets Make sure that it fits securely and that the drain outlet is located at the left rear of the tray Attach one end of the drain tubing reference 470343706
26. e running on a Gilson Keypad Controller You create the executable file using the Gilson 709 Sampler Manager Software ordered separately reference 2106243 If you have not already done so install 735 Sampler Software or 709 Sampler Manager Software Refer to the User s Guide included with the software 3 1 Start Up Operation Start Up To start the sample changer status 1 Make sure the sample changer is connected to a light power source 2 Turn on the sample changer using the power switch located on the rear panel The status light on the front panel becomes lit If the status light begins blinking there is a problem with the sample changer See page 5 6 3 2 2 gt Operation Running Programs The sample changer is controlled by programs executed from a personal computer or Gilson Keypad Controller Program for Windows NT users sweibojg Huluuny The computer is connected to the sample changer via an RS 232 cable Refer to Section 2 Installation for correct installation of the RS 232 cable between the sample changer and the computer The following utility program is supplied on the 223 Utility Program CD ROM supplied with your sample changer 223 Setup Utility Specifies configuration parameters for the sample changer Programs for Gilson Keypad Users The following programs are copied to your computer s disk as part of the 709 Sampler Manager Software installation procedure and ca
27. e the plate from the front of the horizontal arm by removing its three screws 4 Locate the hexagonal shaped control rod by looking down into the horizontal arm Lightly press on the rod with your finger and push the rod towards you When the end of the rod is no longer flush with the end of the horizontal arm grasp the rod and completely remove it 5 While holding the vertical arm loosen the two screws on the underside of the horizontal arm that secure the vertical arm Remove the vertical arm 6 Re insert the control rod as far as it will go While inserting the rod you may need to rotate the rod back and forth slightly to get it to pass through the vertical arm s gearing and motor drive socket 7 Re attach the plate to the front of the horizontal arm Troubleshooting Troubleshooting Error Description 15 NV RAM checksum is 20 21 22 23 24 25 26 27 28 29 5 2 invalid X motor position error Y motor position error Z motor position error X sensor inactive Y sensor inactive Z sensor inactive X target position out of range Y target position out of range Z target position out of range X offset out of range Error Messages Solution Send the buffered 9 GSIOC command to reset the NV RAM and initialize to the default value Run SET_223 EXE or the 223 Setup Utility program to check configuration options Replace the NVM Cycle power to the sample changer
28. eed to obtain a different probe holder and guide for the probe See Appendix A 1 Remove the transfer tubing s 1 4 28 fitting from the tubing fitting 2 Loosen the probe retaining screw and remove the current probe and tubing fitting 3 Unscrew the current probe guide from probe foot Then screw the new probe guide into place 4 Remove the screw securing the probe holder to the bracket and then pull the probe holder from the bracket Insert the new probe holder into the bracket and secure using the screw 5 Install the new probe into the tubing fitting Slide the probe assembly into the probe holder making sure that the probe is centered over the probe guide Secure the probe by tightening the probe retaining screw 6 Re attach and tighten the transfer tubing s 1 4 28 fitting A Maintenance Replacing a Fuse A blown fuse may indicate the existence of another problem in the instrument If the replacement fuses blow do not try others S led Duejd y To change a fuse follow these steps 1 Disconnect the power cord from the power outlet and from the rear panel receptacle 2 Locate the fuse drawer on the rear panel See page 2 3 if necessary 3 Insert a small screwdriver into the notch located at the left of the fuse drawer 4 Twist the screwdriver to open and remove the fuse fuse drawer The fuse drawer contains one 2 0A drawer T Slo Blo fuse 5 x 20 mm size for a 100 120 voltage selection It
29. ertical Arm Installation Vertical Arm Installation Before installing or changing the vertical arm check that the power is turned off and the power cord is disconnected from the power socket Also ensure that the arm locking screw has been removed and the horizontal arm can move To install the vertical arm 1 Remove the cover plate from the front of the horizontal arm by removing its three screws 2 Locate the hexagonal shaped control rod and horizontal slider by looking down into the horizontal arm See first diagram below 3 Using your finger press on the control rod where it passes through the horizontal slider At the same time pull the probe foot towards the front of the horizontal arm This causes the white plastic plug and control rod to move forward slightly When the white plastic plug is no longer flush with the front of the horizontal arm remove it and the control rod from the horizontal arm 4 Pull the probe foot towards the front of the horizontal arm as far as it will go cover plate control rod B a a oo plastic plug 2 16 Installation 10 11 Position the vertical arm onto the horizontal slider When viewed from the front of the sample changer the vertical arm s probe holder bracket is on the right Use the supplied screws to lt A secure the vertical arm to the d fl probe horizontal slider The screws holder insert into the bottom of the bracket mounting holes in the
30. ger It incorporates an EIA RS 485 interface and allows up to 32 slave devices to be controlled from a single master device Each slave device is identified by a unique number that must be known to the device and to the master device The default ID code of the sample changer is 10 To control the sample changer via the GSIOC interface you need the following e a Personal Computer with Microsoft Windows 98 or NT and any Gilson control software or 706 Device Driver Software installed e an unused RS 232 communication port From the computer you e specify the sample changer as the instrument to control by indicating its unit ID e issue commands that set operating parameters control operation or request information from the sample changer C 1 GSIOC Commands Appendix C2 Command Type I GSIOC Commands There are two kinds of commands that you can send over the GSIOC Buffered commands send instructions to the sample changer These commands are executed one at a time e Immediate commands request status information from the sample changer These commands are executed immediately temporarily interrupting other commands in progress GSIOC Command List In the command list below the GSIOC command must be entered in the proper upper or lower case format If a buffered command requires additional information you see italicized text next to the command The description of the command identifies what you nee
31. go It is designed to fit snugly into its receptacle Connect the opposite ends of the wires to the other device s Be sure to match GROUND connections Label each cable to identify the purpose of the connection N terminal block OOOO LIL connector 2 conductor cable 9e U0D nd nopndu to peripheral equipment 2 13 Installation Power Cord 2 14 Power Cord Locate the appropriate power cord for your line voltage Discard the other power cord Use the power cord to connect the sample changer to an AC power source Installation Arm Locking Screw Removal During shipment a screw locks the horizontal arm into place 1 Locate and remove the black plastic plug located on the right side panel of the sample changer With your left hand hold the horizontal arm into place Using a Phillips screwdriver remove the arm locking screw located inside the sample changer Insert the arm locking screw into its storage location on the rear panel Replace the plastic plug on the side panel Ensure that the horizontal arm can move by pushing it to the left as far as it will go Before packing the sample changer for shipment always secure the horizontal arm using the arm locking screw arm locking screw horizontal arm m ae plastic plug j i plastic plug 4 Jeaoway M219S Huy 07 wy arm locking screw 2 15 Installation 2 V
32. horizontal slider You may need to move the vertical arm back and forth slightly to align its mounting holes with those in the horizontal slider Re insert the control rod as far as it will go While inserting the control rod you may need to rotate it back and forth slightly to get it to pass through the gearing and ontal motor drive socket in the slider horizontal slider Applying pressure at the vertical arm s base push the vertical arm to the back of the horizontal arm While slightly moving the probe holder bracket up and down push the control rod until it clicks into position Re insert the white plastic plug Re attach the cover plate to the front of the horizontal arm uo ejjesuj wy eoIWO A 2 17 Installation 2 Probe Installation Probe Installation Locate the accessory packages containing the probe and the probe holder guide kit When installing the probe refer to the following diagram that shows where the probe probe holder and probe guide are installed on the sample changer probe holder bracket and screw robe Folder tubing fitting probe retaining screw probe L o O o probe guide Q Installing Probe Guide To install the probe guide wasner 1 1 a the metal lock washer onto the probe probe ide sase N o 2 Screw the probe guide with lock washer into place on the bottom side of the probe foot 2 18 Installation Installing Probe
33. ion contains some general guidelines for maintaining your sample changer 4 1 Maintenance Cleaning the Sample Changer 4 2 Cleaning the Sample Changer The sample changer should be cleaned occasionally using a dry clean cloth Or if necessary use a cloth dipped in soapy water If liquid is accidentally spilled on the sample changer wipe the instrument using a dry clean cloth Maintenance Replacing Parts Replacing the Probe Refer to the appropriate instructions below depending on whether your replacing the probe with one of the same type or one of a different type Installing same type of probe To install a replacement probe of the same type that is currently installed 1 Remove the transfer tubing s 1 4 28 fitting from the tubing fitting Loosen the probe retaining screw and remove the probe and tubing fitting from the probe holder Remove the old probe from the tubing fitting and insert the replacement probe Slide the probe assembly into the probe holder making sure that the probe is centered over the probe guide Secure the probe by tightening the probe retaining screw Re attach and tighten the transfer tubing s 1 4 28 fitting A syed Duejd y transfer tubing probe holder 1 4 28 bracket and Screw fitting probe tubing fitting 4 3 Maintenance Replacing Parts 4 4 Installing different type of probe To install a different type of probe you may n
34. k Installation Microplate Holder To use microplates with the sample changer you must install the microplate holder You can use standard or deep well microplates A bracket must be attached to the microplate holder before the microplate is installed Assembly and installation of the microplate holder depends on the type of microplates to be used Refer to the appropriate instructions below Instructions for standard microplates For standard microplates 1 Align the lower set of holes labeled A in the microplate holder with the holes in the metal bracket Insert the supplied Phillips head screws and tighten 2 If installed remove the polypropylene tray 3 Install the microplate holder in the upper position on the front standoffs using the two knurled screws Note If a support bar is installed install the rack holder in front of the support bar microplate holder bracket N uo1 ejjg amp suj AIoss 29Wy support bar front standoff E microplate holder 2 25 Installation Accessory Installation 2 26 microplate holder bracket Instructions for deep well microplates For deep well microplates 1 Align the upper set of holes labelled B in the microplate holder with the holes in the metal bracket Insert the supplied Phillips head screws and tighten If installed remove the polypropylene tray Install the microplate holder in the l
35. le Probe Kit Probes for 56 mm Vertical Arm Stainless Steel Titanium Adjustable Probe Sleeve Probe Holder Guide Kits Transfer Tubing Rinse Stations and Accessories Control Options Safety Shield Accessories Replacement Parts Appendix B Racks Appendix C GSIOC Commands GSIOC Commands GSIOC Command List Declaration of Conformity Application of Council Directives 89 336 EEC 73 23 EEC Standards to which Conformity is Declared EN61326 EN61000 3 3 EN61000 3 2 EN61010 1 Manufacturer s Name Gilson Inc Manufacturer s Address 3000 W Beltline Highway Middleton WI 53562 EC Office Address Gilson S A S 19 Avenue des Entrepreneurs B P 45 95400 Villiers le Bel France Type of Equipment Laboratory Equipment Model r a e Retiree tees eee or etre CeO A 223 Sample Changer Beginning with Serial Number 199F5001 Month and Year of Manufacture June 1995 I the undersigned hereby declare that the equipment specified above conforms to the above Directives and Standards Place Middleton WI USA Michael Jacquart Issue Date January 1996 Senior Vice President Corporate Technology Development Introduction The Gilson 223 Sample Changer is an XYZ robot that can automate sample handling procedures It is controlled via a program running on a computer or Keypad Controller 1 1 Introduction Unpacking 1 2 Unpacking The 223 Sample Changer arrives with all major components already assemble
36. le changer may be misaligned Perform the position alignment procedure described on page 4 6 Troubleshooting Electrical Problems Input functions not operating Make sure connections into the terminal block connector are secure Make sure the terminal block connector is secure in input output port Check connections for proper pin assignments Be sure pins from external devices are assigned correctly Check polarity of input Inputs should be a contact closure If not it must be TTL level logic activates Confirm that the device supplying the input signal to the sample changer is working Check logic of controlling program Output functions not operating Make sure connections into the barrier strip are secure Make sure the terminal block connector is secure in the input output port Check connections for proper pin assignments Output from the sample changer should be compatible with the device to which it is interfaced Outputs are contact closures Check logic of controlling program SW9 qO g e914 99 9 5 5 Troubleshooting Electrical Problems 5 6 Status light not illuminated Ensure power is turned on Check AC power cord connections Try different AC outlet Check fuse s replace if necessary Check all sample changer connections Status light blinking Check for obstructions to horizontal or vertical arms or probe Restart controlling program Check for error code fro
37. m sample changer Issue buffered 9 command to reset NV RAM and initialize default parameters See Appendix C for more information on GSIOC commands Contact the Gilson Customer Service Department Sample changer blows fuses Contact the Gilson Customer Service Department Troubleshooting Liquid Level Detector Problems Not detecting liquid level Ensure that the liquid level detector cable is plugged in Check if liquid is detectable Liquid level detection works only if there is electrical conductivity in your liquid Liquid level detecting will not work with most non polar liquids For intermediate polarity liquids and polar liquids check the sensitivity setting in the 223 Setup Utility or SET_223 EXE Check that the liquid level sensing switch is in the correct position Follow the liquid level detector cable to where it is plugged in The liquid level sensing switch is to the right of the connector Make sure the switch is in the right position Call the Gilson Customer Service Department if this is caused by faulty circuitry Q1 sw j q01d 10 29 q 1 497 pinbiq 5 7 Troubleshooting Repair and Return Policies Repair and Return Policies Before calling us Gilson Customer Service personnel will be able to serve you more efficiently if you have the following information e the serial number and model number of the equipment involved e the installation procedure you used e list of concise symptoms
38. n be used if you are controlling the sample changer via the Gilson keypad Note 709 Sampler Manager Software will not run under Windows NT The Pascal versions of these programs are also copied to the computer as part of the softwares installation You can modify them as required using the software SET_223 EXE CONTACT EXE XYZ_TEST EXE POSN_223 EXE 3 3 Operation Running Programs 3 4 Running Programs from the Keypad A Gilson Keypad Controller can be used as an alternate interface between the user and the sample changer The keypad is connected to the sample changer via a coiled cable Refer to Section 2 for correct installation of the cable to the Keypad port located on the sample changer s rear panel The keypad consists of e an 8 line 40 character display white characters on a blue background numeric keypad with an ENTER key four cursor direction keys four programmable soft keys three function keys HELP ESC CLEAR 31 2 disk drive with protective cover Supplied with the 709 Sampler Manager Software installation disks is the 223 Keypad disk that contains the programs listed on page 3 3 and the files listed below In order to run an executable file from the keypad the disk on which the program is stored must contain the following files MGSIOC SYS AUTOEXEC BAT CONFIG SYS MENU EXE An optional program to set the time and date is also provided on the disk but is not required to run programs c
39. n this configuration a 506C System Interface is connected to a computer via an RS 232 cable The sample changer and other GSIOC compatible modules are connected to the system interface by linking GSIOC cables If necessary use the GSIOC cable ordered separately reference 36078143 to connect the sample changer to the GSIOC Use the diagram below as a reference nan Z d GSIOC module z CT mm GSIOC module sample changer E GSIOC 0 system cable interface Installation 2 Changing Termination Switch If you are controlling the sample changer via a GSIOC connection you must change the position of the termination switch The termination switch is inside of the sample changer and has three positions break passive no termination and break active The default position is break passive which is appropriate if you are controlling the sample changer via an RS 232 connection However for GSIOC control you must place the switch in the no termination position To change the position of the termination switch 1 Turn off the power to the sample changer 2 Locate the access slot in the rear panel Refer to the following diagram 223 rear is 33 eeo E Aero Access slot GSIOC for changing connector termination switch 3 Inserta small screwdriver into
40. nfirmation on the target site 3 If the probe is not aligned over the target location use the Manual Nudge arrow buttons to move the probe in the appropriate X or Y direction 4 To store changes to the X and Y offsets click Ok Or to display the X and Y offsets currently set in the instrument s memory click Cancel 5 Click Home to home the instrument Maintenance Transporting the Sample Changer When moving the sample changer 1 Re install the arm locking screw using the following procedure Refer to the diagrams on page 2 7 if necessary f Turn off the sample changer Move the horizontal arm to the right as far as it will go Locate and remove the arm locking screw from its storage location on the rear panel Remove the plastic plug located on the right side panel While holding the horizontal arm in its far right position install the arm locking screw into place inside the sample changer Replace the plastic plug 2 Lift the sample changer by grasping the base Do not use the horizontal arm as a handle A JaBueyy jdwes y BurodsuelL 4 7 Maintenance Transporting the Sample Changer 4 8 If packaging the sample changer for shipment remove the vertical arm To remove the vertical arm refer to the diagrams on pages 2 16 and 2 17 and follow the instructions below 1 Disconnect the transfer tubing from the probe 2 Loosen the probe retaining screw and remove the probe 3 Remov
41. nning or E for error Appendix Yyyyy zpppLs Zzzzz s Reads the Y axis location of the probe based on the microstep position Returns yyyy which is the loca tion in tenths of millimeters Sets new Y axis position for the probe where yyyy Y axis position in tenths of millimeters Reads Z motor status Returns one of the following spuewiwod OISD U for unpowered P for powered R for running or E for error For use with liquid level sensing sets new Z axis position for the probe pppp Z axis position in tenths of millimeters If liquid level detection is on movement of the probe is stopped if liquid is detected before this position is reached s Speed index of 1 to 5 default is 4 See table below s 1 2 3 4 5 Speed mm sec 19 9 30 2 default for liquid level sensing 61 8 126 9 default for normal travel 247 3 Reads the Z axis location of the probe based on the microstep position Returns zzzz which is the loca tion in tenths of millimeters Sets new Z axis position for the probe where zzzz Z axis position in tenths of millimeters Even if liquid level sensing is on the probe is moved to this position s Speed index of 1 to 5 default is 4 See table above CF
42. on Serial Cable Adapter 9 pin 25 pin sallossedoy puke Sed jUOWAeDR dey 223 Safety Shield kit Encloses sides and front of 223 working area Filler port to transfer samples from the 223 Sample Changer to another instrument 0 3 mm ID Teflon Tubing 10 feet plus 2 end fittings To connect filler port to other instrument 0 8 mm ID Teflon Tubing 10 feet plus 2 end fittings To connect filler port to other instrument Transfer port accessory set for 1 port Includes one polyamide molded transfer port PTFE inlet tubing 1000 x 3 x 2 mm ID with filter PE disposable sealing caps amp instruction leaflet Replacement transfer port does not include inlet tubing extra sealing caps or instruction leaflet Sealing caps for transfer ports package of 1000 Diverting valve assembly for 223 Sample Changer Includes mounting bracket tubing and two tubing cable clips Diverting valve for 223 Sample Changer Tubing to connect diverting valve and 223 probe Appendix Replacement Parts and Accessories A 6 Replacement Parts 1907141 27072001 23074002 190712 190713 2507023 638308513 638310513 709910206 36078143 6730204007 36083122 Support bar for mounting rinse station s transfer port s and filler port Probe connecting nut for 1 5 mm probes Probe connecting nut for Teflon probe Level sensing cable Tubing cable support rod Tubing retaining clip for support rod Terminal block connector 8 pin Terminal block c
43. on Z Arm Fe 223 Setup Utility Use the Z Arm tab to identify the size of the installed Z Arm Setting the size of the Z Arm controls the amount of travel in the Z axis To store the selected Z height settings to the sample changer s memory click Ok To display the Z height settings currently set in the sample changer s memory click Cancel oo suondo uoneinbyuoy Buryoeyy Operation Checking Configuration Options 3 12 Contacts Fe 223 Setup Utility Jv Open j Open Jv Open Jv Open The Contacts tab allows you to toggle output contacts to determine if the correct contact connections have been made to peripheral devices to be controlled by the 223 Sample Changer Before using this tab you need to connect the peripheral device s inputs to the appropriate output pair on the sample changer If necessary refer to Section 2 for information on making contact connections To exit the software click on Done Operation Error Handling Fe 223 Setup Utility BIEar Error This tab displays the current error when the instrument is in an error state For error solutions refer to Section 5 Troubleshooting in the 223 Sample Changer User s Guide To clear the error click Clear error Click Reset NV RAM to reset the configuration options to the factory defaults Click Run XYZ to demonstrate the capabilities of the sample changer by performing an XYZ test The XYZ test moves
44. onnector 10 pin 2 conductor interconnect wire 6 Shielded GSIOC cable 30 Fuse 2 amp 250V T type 5 mm x 20 mm Serial cable IBM AT type 9 pin female to 25 pin male Racks B You can configure the sample changer with a variety of rack types and sizes The sample changer can hold a maximum of four Code 20 Series racks or thermostated Code 30 Series racks B 1 Appendix N Code 0 rack For 80 vessels Material polypropylene Vessels and maximum capacity 12 x 32 mm tubes 9 ml Reference number 270430 Code 7 rack holder For one 96 well microtiter plate Vessel capacity 0 3 ml well Reference number 2707401 Note Requires installation of rack holder reference 1907143 See Section 2 Code 8 rack For 120 vessels Material polypropylene Vessels and maximum capacity 6 x 32 mm tubes 0 3 ml Reference number 270438 Code 9 rack For 120 vessels Material polypropylene Vessels and maximum capacity 7 x 40 mm tubes 0 7 ml Reference number 270439 Note Requires installation of rack holder reference 1907143 See Section 2 Code 20 rack For 108 vessels Material polypropylene Vessels and maximum capacity 10 x 100 mm tubes 4 5 ml Reference number 150425 Appendix B Code 21 rack For 60 vessels Material polypropylene Vessels and maximum capacity 13 x 100 mm tubes 9 ml Reference number 150422 Code 22 rack For 44 vessels Material polypropylene Vessel
45. ontrolling the sample changer Files for this optional program are DAYTIME EXE and DAYTIME TXT To copy your sample changer program s onto the keypad disk use the Windows Explorer in Microsoft Windows Operation The MENU EXE program allows you to view and select one of up to 100 executable files stored on the disk If you have more than 100 files and you want to maintain your keypad disk move the utility programs and example programs to another disk or to a computer for storage To run an executable file enter the number of the program corresponding to the menu selection and press ENTER To stop a program follow the program specific instructions on stopping the program If a program prompts you for a yes or no Y N response press 1 on the keypad to indicate yes or 0 to indicate no sweiGoig Huluuny 3 5 Operation Checking Configuration Options 3 6 3 Checking Configuration Options The sample changer comes from the factory with its configuration already set Configuration information is stored in the non volatile memory NV RAM of the sample changer Prior to using the sample changer for the first time review and adjust the default configuration to ensure it is correct for your setup The 223 Setup Utility program allows you to review and if necessary change the configuration options set for the sample changer When you execute the 223 Setup Utility from the computer the following tabs appear About
46. overwrite unexecuted existing commands If you send this command without indicat ing a parameter mm the buffer is cleared C5 GSIOC Commands Appendix C4 VZZZZ SSS Xxxxx yyyy w C For tracking liquid height raises or lowers the Z height of the probe at the designated speed zzzz Z height in tenths of millimeters sss speed in tenths of millimeters per second at which to move the probe Reads diverting valve status Returns one of the follow ing 0 Valve status off the port connected to the probe is the one facing the rear of the sample changer 1 Valve status on the port connected to the probe is the one facing you When you turn off the sample changer the valve status is set to off Sets status of diverting valve For x indicate 1 for on or 0 for off See V command above for a description of aid bad and 0 Reads X motor status Returns one of the following U for unpowered P for powered R for running or E for error Reads the X and Y axis locations of the probe based on the microstep position Returns xxxx yyyy which is X and Y axis locations in tenths of millimeters Sets new X and Y axis positions for the probe where xxxx X axis position in tenths of millimeters yyyy Y axis position in tenths of millimeters Reads Y motor status Returns one of the following U for unpowered P for powered R for ru
47. ower position of the front standoffs below the support bar if installed Use the two knurled screws supplied with the microplate holder support bar front standoff microplate holder Installation Tubing Cable Support Rod The tubing cable support rod restrains excess transfer tubing Place the tubing cable support rod on top of the sample changer A magnet at the bottom of the rod holds it into place on top of the sample changer Snap the transfer tubing between the probe and dilutor into the small grooves on the clips installed on the tubing cable support rod You may need to pry the grooves apart using a flatblade screwdriver before snapping the tubing into place Also remember to leave enough tubing to account for the movement of the probe Gather any excess transfer tubing into a coil to minimize the length of tubing and secure the coil using a twist tie Position the excess tubing in a convenient location near the rear of the sample changer transfer tubing tubing retaining clips tubing cable support rod uo1 ejj amp suj Aossa00y 2 27 Installation Accessory Installation 2 28 support bar connector lt LT tubing 4 Transfer Port To install a transfer port 1 5 Place the transfer port in any mounting hole on the support bar Screw the male tubing connector into the bottom of the transfer port Fit a yellow cap to the top of the tran
48. probe reaches the target site the software relaxes the Z Arm so you can manually raise and lower the probe for visual alignment confirmation on the target site 3 If the probe is not aligned over the target location use the Manual Nudge arrow buttons to move the probe in the appropriate X or Y direction 4 To store changes to the X and Y offsets click Ok Or to display the X and Y offsets currently set in the instrument s memory click Cancel 5 Click Home to home the instrument Operation Arm Travel F 223 Setup Utility The Arm Travel tab displays the XYZ travel in kilometers suondo uoneinbyuoy Bulryoeyy 3 9 Operation Checking Configuration Options 3 10 Liquid Detector Fe 223 Setup Utility The Liquid Detector tab allows you to adjust the sensitivity of liquid detection The factory default setting is 3 Raising the percentage suppresses false liquid detection while lowering the percentage increases sensitivity for situations where liquid is harder to detect By lowering the probe so it touches the liquid for one of your samples you can manually test the current sensitivity of the liquid detection In this manner you can observe the amount of change you might expect with each sample To store the sensitivity setting to the sample changer s memory click Ok To display the sensitivity setting currently set in the sample changer s memory click Cancel Operati
49. rack for 36 Waters WISP vials Material aluminum Vessels and maximum capacity 15 x 45 mm 4 ml Reference number 2704434 Code 34P rack For 36 Waters WISP vials Material polypropylene Vessels and maximum capacity 15 x 45 mm 4 ml Reference number 2704534P Note Requires installation of rack holder reference 1907142 See Section 2 Code 35P rack For 71 vessels and one scintillation vial Material polypropylene Vessels and maximum capacity 12 x 32 mm 2 ml 28 x 60 mm 20 ml Reference number 2704535P Note Requires installation of rack holder reference 1907142 See Section 2 syoey B 7 Appendix B Code 36P rack For 120 microvials and one scintillation vial Material polypropylene Vessels and maximum capacity 7 x 40 mm 0 7 ml 28 x 60 mm 20 ml Reference number 2704536P Note Requires installation of rack holder reference 1907142 See Section 2 oo Code 37 rack i LERE EEn Deseas Thermostated rack for 56 vessels and one scintillation vial Material aluminum Vessels and maximum capacity 12 x 32 mm 2 ml 28 x 60 mm 20 ml Reference number 2704437 Code 38 rack Thermostated rack for 96 microvials and one scintillation vial Material aluminum Vessels and maximum capacity 7 x 40 mm 0 7 ml 28 x 60 mm 20 ml Reference number 2704438 B 8 GSIOC Commands C The GSIOC is a communications interface that enhances the power of the sample chan
50. s and maximum capacity 18 x 150 mm tubes 25 ml Reference number 150424 Code 22U rack For 44 vessels Material polypropylene Vessels and maximum capacity from 10 x 75 mm tubes 3 5 ml to 18 x 180 mm tubes 32 ml Reference number 150498 Note Each of the reception cavities contains four positioning and retaining clips Code 23 rack For 44 vessels Material polypropylene Vessels and maximum capacity 17 x 55 mm vials 6 8 ml 17 x 65 mm vials 8 ml Reference number 150426 Appendix B 4 Code 23W rack For 44 Waters WISP vials Material polypropylene and stainless steel Vessels and maximum capacity 15 x 45 mm 4 ml Reference number 150426 Code 24 rack For 14 scintillation vials Material polypropylene Vessels and maximum capacity 28 x 60 mm 20 ml Reference number 150427 Code 28 rack For 108 vessels Material polypropylene Vessels and maximum capacity 10 x 65 mm tubes 3 ml 10 x 75 mm tubes 3 5 ml Reference number 150420 Code 29 rack For 60 vessels Material polypropylene Vessels and maximum capacity 12 x 75 mm tubes 5 ml 13 x 75 mm tubes 6 ml Reference number 150429 Appendix B Code 29 LE rack syoey For 60 large 1 5 ml Eppendorf vials Material polypropylene and stainless steel Vessels and maximum capacity 11 x 40 mm 1 5 ml Reference number 2704342 Code 29 SE rack For 60 small 0 5 ml Eppendorf vials Material polypropylene and s
51. sfer port Connect the other end of the tubing to a filter and place it in a solvent or sample bottle Make sure the solvent or sample bottle is at the same level as or higher than the sample changer s tray Fix the plastic clip onto the transfer port Using transfer ports Be aware of the following when using transfer ports Place the solvent or sample bottle at the same level as or higher than the tray Use a beveled edge constricting probe Prime each transfer port before use Installation Filler Port When a sample is prepared it can be injected into a peripheral instrument via a filler port which can be installed on the support bar Refer to the diagram when installing the filler port 1 Separate the probe guide from the filler port assembly by unscrewing it The probe guide has a hexagonal head 2 Insert the probe guide into a mounting hole on the support bar and tighten 3 Attach the bottom portion of the filler port assembly to the probe guide G a N uo1 ejjg amp suj AIoss 29Wy 2 29 Installation Accessory Installation liquid level detector cable probe retaining screw 2 30 Liquid Level Detector The liquid level detector detects when the probe has reached the liquid surface This permits the program controlling the sample changer to calculate the change in the liquid level so the tip of the probe remains a predetermined distance below the liquid
52. tainless steel Vessels and maximum capacity 7 x 30 mm 0 5 ml Reference number 2704341 Code 30 rack For 60 vessels Material aluminum Vessels and maximum capacity 12 x 32 mm vials 2 ml Reference number 2704430 Code 30P rack For 60 vessels Material polypropylene Vessels and maximum capacity 12 x 32 mm vials 2 ml Reference number 2704530P Note Requires installation of rack holder 1907142 See Section 2 B 5 Racks Appendix B 6 Code 31 rack Thermostated rack for 108 vessels Material aluminum Vessels and maximum capacity 7 x 40 mm vials 0 7 ml Reference number 2704431 Code 31P rack For 108 vessels Material polypropylene Vessels and maximum capacity 7 x 40 mm tubes 0 7 ml Reference number 2704531P Note Requires installation of rack holder reference 1907142 See Section 2 Code 32 rack Thermostated rack for 60 vessels Material aluminum Vessels and maximum capacity 13 x 65 mm tubes 6 ml 13 x 100 mm tubes 9 ml Reference number 2704432 Code 33 rack Thermostated rack for 14 scintillation vials Material aluminum Vessels and maximum capacity 28 x 60 mm 20 ml Reference number 2704433 Appendix Code 33P rack For 14 scintillation vials Material polypropylene Vessels and maximum capacity 28 x 60 mm 20 ml Reference number 2704533P Note Requires installation of rack holder reference 1907142 See Section 2 Code 34 rack Thermostated
53. ter For the location of the RS 232 port refer to the diagram on page 2 3 Using the computer you can run programs that control the sample changer See Section 3 for more information Be sure your computer is turned off before making any connections To connect your computer to the sample changer you need an RS 232 cable Obtain a cable with D connectors that are appropriate for the sample changer and your computer The sample changer requires a 25 pin male D connector Refer to the rear panel of your computer or its documentation to determine which type of D connector it requires Connecting the RS 232 Cable Attach the male end of the RS 232 cable to the RS 232 port located on the rear panel of the sample changer Tighten the retaining screws Attach the other end of the RS 232 cable to the computer s RS 232 port Do not mistake it for the female 25 pin parallel printer port Again tighten the retaining screws female D connector connect to computer male D connector connect to sample changer 2 5 Installation Keypad 2 6 Keypad The Keypad port is used to connect a Keypad Controller to the sample changer Connect the keypad s coiled cable to the sample changer s Keypad port For the location of the Keypad port refer to the diagram on page 2 3 Using the keypad you can run programs that control the sample changer See Section 3 for more information Installation GSIOC The GSIOC
54. than 5V DC to an input When using TTL signals be sure to match GROUND connections Outputs The sample changer has connections for five outputs All of the outputs are paired Pins 1 and 2 supply ground and a 12V DC output Do not use this output unless the receiving device can accept 12V power Do not allow more than 500 mA load Pins 3 through 10 are paired isolated relay contact closures and are labeled 1 2 3 and 4 Items You Need to Make Connections To make connections you need the following e 2 conductor cable 22 30 gauge for each wire e wire insulation stripper e small blade screwdriver Installation Making Connections To prepare and make connections with the 2 conductor cable 1 2 Cut the cable into pieces of appropriate length Strip about 1 1 cm of insulation from each end of the cable Locate the appropriate green terminal block connector in the accessory package The connector for inputs has eight slots while the one for outputs has ten Insert each wire into the appropriate slot on the terminal block connector Push the wire all the way in then tighten its corresponding pin screw Note When making connections be sure to maintain the correct orientation of the terminal block connector relative to the port This is especially important if making connections to the 12V DC output Connect the terminal block connector to the sample changer Push the connector in as far as it will
55. the access slot 4 Use the screwdriver to move the switch from its default left position break passive position to the center position no termination position The right position is the break active position Refer to the following diagram Termination switch Break ii i i Break No passive No active termination JOISS Installation 2 Alternate method for changing the termination switch GSIOC If your sample changer does not have an access slot on its rear panel follow these steps to change the position of the termination switch 1 Turn off power and disconnect the power cord to the sample changer 2 Remove the nine screws that secure the rear panel and then remove the rear panel Refer to the following diagram Rear panel Hees 6 eee 3 Locate the control panel It includes the sample changer s connectors and switches Refer to the following diagram ie Nie ee ee ee ieee _ _ oe am A ee b pe eee a o pera a a p Loosen these E a 3 nuts Control panel 2 10 Installation 4 Loosen the three internal nuts that secure the control panel to the front of the sample changer And remove the two screws that secure the control panel to the back side of the sample changer 5 Remove the control panel by tilting it downward to free it from the three screws that secure it to the front of the sample changer And then pull the control
56. the bolts the plastic side faces outward Use a 5 16 wrench to fully tighten the nuts Snap the diverting valve s cable into the tubing cable clip attached to the horizontal arm Then connect the cable to its connector on the front face of the sample changer Since the top port of the diverting valve is the common port attach one end of the supplied tubing to the top port and the other end to the probe Snap the tubing into the tubing cable clip attached to the back of the vertical arm If necessary lower the probe so you can route the tubing through the clip Attach the transfer tubing from each dilutor to the side ports smaller tubing cable clip horizontal arm cad 00O larger tubing cable clip tubing diverting valve nuts A N gt O O iy n 3 5 5 D fe l vertical arm o l lt probe ie mounting bolts tubing cable clips diverting valve connector Unit ID and Baud Rate Selection Installation 2 32 Unit ID and Baud Rate Selection Use the SW1 selector to choose a different unit ID and the SW2 to choose a different baud rate These selectors are located on the sample changer s rear panel see page 2 3 if necessary Unit ID The unit ID identifies the sample changer to software packages that can issue GSIOC commands to the sample changer At the factory the unit ID
57. to the drain outlet and place the other in a drain receptacle located lower than the tray Upper Position If using tubes that are less than 100 mm in height 1 Screw the tray spacers into place See diagram below Note When not being used screw the tray spacers into the holes located in the left rear of the sample changer s bed 2 Place the tray on top of the tray spacers making sure it fits securely in the tray mounting brackets and that the drain outlet is located at the left rear of the tray Attach one end of the drain tubing reference 470343706 to the drain outlet and place the other in a drain receptacle located lower than the tray uolzeyyeysuy Aes 2 21 Installation o 2 Tray Removal To remove the tray first lift it straight up and then bring it towards you Tray Installation tray spacers storage upper position lower position tray spacers for use with tray installed in tray mounting upper position brackets 2 22 Installation Rack Installation The sample changer is equipped to hold up to four Code 20 series or thermostated Code 30 series racks Place each rack into the tray so the rack is perpendicular to the front panel of the sample changer The rack code assigned to the rack should face you See Appendix B for a list of racks available for the sample changer To use a Code 0 7 8 or 9 rack you must install rack holder reference 1907143
58. ts return and shipping charges if your institution requires it Ship to Gilson Inc Attention Customer Service indicate the authorization here 3000 W Beltline Highway Middleton WI 53562 Outside the United States contact your Gilson representative for return procedures SdlD1 Og UIN JeY pue seday 5 9 Replacement Parts and Accessories A For reference numbers for available racks refer to Appendix B VerticalArms 190610 183 mm vertical arm 190611 123 mm vertical arm 190613 56 mm vertical arm Probes for 183 mm Vertical Arm Stainless Steel 27067361 Beveled edge probe not septum piercing 220 5 x 1 5 x 1 1 mm 27067373 Non septum piercing probe constricted tip capacitive level sensing stainless steel Dimensions 221 x 1 5 x 1 1 mm ID tip dimensions 2 x 1 1 x 0 4 mm ID 27067374 Non septum piercing probe constricted bevel tip capacitive level sensing stainless steel Dimensions 221 x 1 5 x 1 1 mm ID tip dimensions 2 x 1 1 x 0 4 mm ID 27067362 Side entry probe septum piercing 220 5 x 1 5 x 1 1 mm Teflon 27067369 Teflon coated probe for non metal applications 220 x 0 9 mm ID Appendix Replacement Parts and Accessories A 2 Probes for 123 mm Vertical Arm Stainless Steel 27067211 27067213 27067212 27067272 27067235 27067236 27067231 27067232 27067276 27067277 27067238 Titanium 27067237 27067236 Beveled tip probe not septum piercing

223 Sample Changer User's Guide

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