306 User's Guide
Contents
1. GSIOC Control Appendix B Buffered R Set Refill Time Syntax Rn Parameter n is in the range 125 to 1000 The unit is the millisecond Any intermediary value is valid Immediate R Request Refill Time Response format a 4 digit decimal number in the range 0125 to 1000 Returns the current refill time in milliseconds Buffered d Set Dispense Speed Syntax dn Parameter n is in the range 0 to 12272 The unit is the ten thousandth of the maximum flowrate of the pump head Command example d1000 With a 5 ml min pump head the selected dispense flowrate is thus 5 1000 10000 0 5 ml min Comment this command does not initiate liquid flow cf B command Immediate d Request Dispense Speed Response format a 5 digit decimal number in the range 00000 to 12172 Returns the current dispense speed as define above Buffered s Set Flow Speed Syntax sn Parameter n is in the range 0 to 12272 The unit is the hundredth of percent of the maximum flowrate of the pump head Command example s2000 With a 25 ml min pump head the selected flowrate is thus 25 2000 10000 5 ml min Comment this command initiates liquid flow Immediate s Request Flow Speed Response format a 5 digit decimal number in the range 00000 to 12172 Returns the current flow speed as defined above Buffered v Set Dispense Volume Syntax vn Parameter n is in the range 0 to 1000000 The unit2. Set Dispense Mode Syntax D Comment this command sets the pump in Dispense mode it does not initiate liquid flow Appendix B 3 GSIOC Control Appendix B GSIOC Commands Buffered F Set Flow Mode Syntax F Comment this command sets the pump in Flow mode it initiates liquid flow according to valid parameters Buffered L Lock Pump Syntax L Comment this command sets the pump in the remote control mode When the pump is locked the PRIME switch is disabled and the REMOTE led is turned on Buffered S Stop Flow Syntax S Comment this command stops the liquid flow if the pump is in Flow mode It has no effect when the pump is in the Dispense mode Buffered U Unlock Pump Syntax U Comment this command restores the local control mode The PRIME switch is enabled again and the REMOTE led is turned off Immediate Z Master Reset or Response format Z or is echoed Appendix B 4 Emulation commands This set of commands is available only when the 306 pump has been set in emulation mode by the L buffered command It allows the programmer to control the 306 pump with a larger range of parameters Command Type Function Set Refill Time Request Refill Time Set Dispense Speed Request Dispense Speed Set Flow Speed Request Flow Speed Set Dispense Volume Request Dispense Volume Set Compressibility Stroke N lt lt novnvaa DJD U UU UU U U
3. For safety reasons do not connect the power cord until you have finished assembling the instrument OO JOMOd uONEIjEISUJ 891119913 3 3 Installation Mechanical Installation 3 4 Mechanical Installation This section explains how to install the pump head the mast clamp and mast The pump head and mast clamp for each pump should be installed before positioning the modules Pump Head Installation The pump head is shipped in a hard case to protect it during transit Unpack the pump head from its case and check that all of the parts are included Follow the procedure below to install the pump head e Insert the pump head into the front aperture of the pump Seethe opposite figure The notch at the bottom of the pump head body must be fitted onto the matching pin on the pump just below the aperture This notch ensures that the inlet port is on the bottom and the outlet port is on the top Holding the pump head in place with one hand set the clamp diagonally over the head Turn the clamp clockwise into position in the slots on both sides of the pump head e Tighten the thumb screw until the clamp holds the pump head securely Make sure that the clamp ends are secured in their slots on both sides See the pump head User s Guide for more information on the pump head Mast Installation The mast is used to stabilise a system when several modules are stacked on top of each other It can also used to
4. e Turn the valve to the SYRINGE INJECT position Press the PRIME key on the front panel of the 306 The pump will start running at its maximum speed and the prime indicator on the front panel will light Depress the syringe until the pump inlet is clear of bubbles and some liquid has passed through the pump outlet e Turn the valve to the RUN position Remove the syringe from the prime valve When no bubbles can be seen at the outlet tubing press the PRIME key to end the priming procedure Check that there are no leaks in the system For the 25SC 50SC 100SC 25WTi and 200WTi pump heads eprime the pump directly without syringe or valve Syringe Load position i Pump Inlet Operation Checking that the 306 is properly connected GSIOC to Controller The 306 piston pump is a slave pump It must be connected to a system controller using the GSIOC cable provided Make sure that the 306 is connected to the system controller and that the system controller is powered in which case the remote indicator on the 306 should light up The Gilson 306 pump is now ready to run Return to your manual for the system controller either a computer with Gilson HPLC system controller software or a Gilson 305 Master pump acting as a system controller Proceed with the setting up of your complete HPLC system D 19 011409 0 JOISD paj9auuos Ajsedoud si 90 y 1ey1 Bunmoayy Operation 4 4 Maintenance and Troubleshooting 5
5. The 306 pump has been designed to require a minimum level of care and maintenance In practice maintenance is limited to cleaning and replacing parts of the pump head 5 1 Maintenance and Troubleshooting Pump Head Maintenance 5 2 Pump Head Maintenance The check valves and filters can be cleaned Piston seals check valves piston assemblies anti extrusion gaskets and return springs should be replaced on a regular basis A maintenance kit is available for each model of pump head For details about maintenance kits and procedures see the User s Guide for your pump head The use of equipment for continuous unattended operation is becoming more and more important For this reason the following table gives an indication of replacement periods of maintenance parts according to the type of use intensive regular or occasional The data in the table below assumes that the pump is working at half of its maximum flow rate and pressure The nature of the liquid and the pump head model have only a small influence on these figures Time table for checking replacement parts according to the type of use Parts Use Intensive Regular Occasional 168 h week 40 h week 10h week Piston seal 2 3 months 6 9 months 1 year Set of check valves 3 6 months 1 year 2 years Piston assembly 6 12 months 2 3 years 5 years Anti extrusion gasket 6 12 months 2 3 years 5 years Return spring 1 year 2 3 years 5 years
6. Maintenance and Troubleshooting Troubleshooting Electrical problems Problem Possible cause Solution Pump does not operate and power indicator does not light Power cord unplugged Fuse blown Incorrect voltage setting Check for power See Electrical installation in chapter 3 Slave pump does not operate GSIOC cable not connected or incorrectly connected Incorrect GSIOC identity number Check GSIOC cable is connected correctly to the socket on the 306 Check GSIOC identity number is set correctly Message Pump X missing on the system controller Incorrect GSIOC identity number set in pump X Set the GSIOC identity correctly Refer to chapter 3 Remote indicator does not light up GSIOC cable not connected Incorrect GSIOC identity number Connect the 306 pump to the system controller using the GSIOC cable provided and switch on controller Check GSIOC identity number is set correctly Hydraulic problems Problem Possible cause Solution Leaks from the hole at the bottom of the pump head Defective piston seal Replace piston seal Refer to User s Guide for the pump head Low flow rate Leaks Plugged inlet filter Defective check valve Pump head not mounted properly Check for leaks Clean or replace the inlet filter Refer to User s Guide for the pump head Clean or replace the check valve R
7. hold the prime purge valve and a manual injection valve The mast clamp should be installed before positioning the 306 in a system The mast is added after all of the modules have been put in place Follow the procedure below to install the mast clamp e Remove the side screw holding the module cover See the opposite figure e Screw on the mast clamp Installation Fix one clamp onto each pump in the system After all the modules have been positioned the stainless steel mast can be secured within the clamps The lower end of the mast should be level with the bottom of the lowest pump oO uonejjelsuj e9 Uuey99aN 3 5 Installation 4 Hydraulic Connections 2 Connect the 306 pump head input with the inlet 6 tubing assembly provided with the pump head The connections to the 306 pump head output should be O made using stainless steel or titanium tubing 3 y Thefollowing figures show the hydraulic connections for different HPLC system configurations TI 3 6 Mains on 306 Pump Outlet port Remote Inlet port Installation Positioning the Modules Before putting each modulein position make sure that each module is ready i e that the fuses have been installed and that any mechanical installation is finished The physical positioning of each of the modules in your system will depend on your type of system and the system controller Som
8. is necessary to use a Gilson manometric adapter which converts the pressure signal from the 802 803 804 module into a signal that can be used by the system controller for the 306 pump The system will have the specified flow rate accuracy of the 306 as the system controller receives a pressure signal The pressure is displayed on both the system controller and the manometric module The pressure limit s are set in the system controller software The pressure controls on the manometric module have no effect 1st Generation Pumping Systems Appendix D Using a 306 with a 302 303 When using a combination of 302 303 and 306 pumps the message Invalid settings may appear when using the Pressure bar Maximum Flow rate ml min pump near its maximum flow rate This is due to the controller asking a 302 303 motor to deliver more than it is capable of giving because the 302 303 pump has a lower maximum motor speed 126 rpm than the 306 pump 150 rpm A 306 pump can accurately produce the maximum flow rate for a given headsize up to the maximum pressure For example with a 5SC head the 306 can produce 5 ml min up to 600 bar The 302 303 pumps can NOT accurately produce the maximum flow rate up to the maximum pressure An example using n Hexane and a 303 pump is given in the above table This liquid is one of the more compressible liquids and therefore one of the extreme cases If you program a flow rate for a 302
9. is the ten thousandth of the pump head nominal number for a 10 ml min head the dispense volume range is thus from 0 001 to 1000 ml Immediate v Request Dispense Volume Response format a7 digit decimal number in the range 0000000 to 1000000 Returns the current dispense volume as defined above Buffered z Set Compressibility Stroke Syntax zn Parameter n is in the range 0 to 10000 The unit is in ten thousandth of the stroke volume spuewWwog DOISD Appendix B 5 GSIOC Control Appendix B GSIOC Commands Specific commands This set of GSIOC commands is specific to the Model 306 pump It is used by the controlling device in a multi pump solvent delivery system Below is the list of available commands in alphabetical order All commands are detailed below with their type mode and function Immediate commands have their response format described Buffered command parameters are documented Comments are added where necessary Command Type Function Request Pump Identification L Request Manometric Module Identification Q B Enter Pressure Unit Q Read Pressure Value q Autozero Pressure Reading Immediate Request Module Identification Response format 306Va bc where Va bc is the software version Immediate L Request Manometric Module Identification Response format 4 character alphanumeric string Response example M806 Comment if no
10. manometric module is present None is returned If the manometric module is not recognized Unkw is returned Buffered Q Enter Pressure Unit Syntax Qu Parameter u is B for bars P for MPa or K for kpsi Command example Qk to select the kpsi unit Immediate Q Read Pressure Value Response format Bxxx in bars Px xx in MPa or Kxx x in kpsi The unit in which the pressure is expressed is the one used by the last buffered Q command At the power up the default unit is the bar Response example K32 1 for 32 1 kpsi Comment if no manometric module present N is returned Immediate q Pressure Reading Autozero Response format q is echoed If autozeroing is not possible converter out of range n is returned Appendix B 6 GSIOC Control Appendix B GSIOC command example In this example we will command the 306 pump to inject 2 ml with a flow rate of 5 ml min A 5SC pump head is used on the injection pump There are 5 steps to follow 1 Lock the pump Buffered L 2 Set the pump in dispense mode Buffered D 3 Send the volume to be dispensed Buffered v 4 Send the flow rate for the dispense Buffered d 5 Start the dispense operation Buffered B Two calculations have to be done the number for the dispense volume and the number for the dispense flow rate For the dispense volume you send the command VXXXXXXX Calculate xxxxxxx dispense v
11. of the 306 with a pump head mounted There are 3 indicators on the front panel The function of each indicator is as follows e Mains on off This indicator is on when the 306 pump is on e Remote indicator This indicator is on when the 306 is under the control of a system controller either a computer or a Gilson 305 Master pump e Prime This indicator is on when the 306 piston pump is being primed Description Rear View The figure below shows a rear view of the 306 with the electrical connectors The function of each connector is as follows GSIOC FROM CONTROLLER Connection to the controller either a computer or a Gilson 305 Master pump MANOMETRIC MODULE Connection to the manometric module REMOTE Use these switches to set the GSIOC identity number Power switch On off power switch Power receptacle Voltage selector and fuse holder MANOMETRIC GSIOC MODULE REMOTE FROM zz CONTROLLER N O MOLA 19H Description 2 4 Installation This chapter describes how to install the 306 It is recommended that you follow the installation instructions in the order that they are presented in the manual 3 1 Installation Electrical Installation Power 3 2 Electrical Installation Power For safety reasons the 306 is shipped without the fuses installed and with the voltage selector in the 220 240 Volt position You must e Insert the correct fuses e Set
12. the output from the manometric module can be connected to the socket marked MANOMETRIC MODULE on any pump in the system Installation Connecting the 306 to other Gilson modules The system controller and the slave pumps in the system are connected together using the Gilson Serial Input Output Channel GSIOC Each module in the system has at least one GSIOC connector on its rear panel When connecting more than two pieces of equipment by GSIOC cable the devices should be connected linearly i e in series using shielded Y type Gilson cables reference 36078143 This type of cable has a pair of female and male 9 pin D connectors at one end and a second female connector at the other You will need one less cable than the number of devices being connected The first pair of devices in the chain are connected using the female sockets at either end of the cable Then for the third and subsequent devices you connect another cable between the male socket on the GSIOC cable and the appropriate socket on the next device Thus you can daisy chain the pumps and other Gilson equipment such as a 15X detector To connect a 306 pump to the system controller you must connect the socket marked GSIOC FROM CONTROLLER on the rear panel of the 306 to the system controller using the GSIOC cable provided The system controller may be a 305 321 331 or 333 Pump or a computer with Gilson HPLC system controller software via a Gilson GSI
13. the voltage selector to your local voltage Inserting the Fuses Ensure that the power cord is not connected before starting to install the fuses Follow the procedure below to install the two fuses e The voltage selector and fuse holder is located under neath the power socket See opposite figure Pull the voltage selector out of the power receptacle This is done by gently levering the selector out using a small screwdriver Pull out the drawer as shown in the figure opposite Insert the first fuse into the clips Push the drawer back into position Pull out the drawer for the second fuse which is on the other side of the voltage selector Insert the second fuse into the clips The instrument requires two fuses to be installed The type of fuses required are 2 0 Amp type T slow blow for 100 120V 1 0 Amp type T slow blow for 220 240 V For safety reasons piston pumps are delivered without fuses installed Fuses must be installed by the user upon delivery Selecting the Voltage The 306 can be set to operate at 100 120 volts or 220 240 volts The different voltages are selected depending on the orientation of the fuse holder To set the voltage to 100 120 volts Insert the fuse holder with the numbers 110 120 on the bottom facing the small white arrow Installation To set the voltage to 220 240 volts Insert the fuse holder with the numbers 220 240 on the bottom facing the small white arrow
14. volatile solvents Maximum accuracy error 1 with water over the full flow rate and pressure ranges Pump Flow rate Pressure Manometric head range range module model ml min MPa model 5SC 0 010 5 0 1 60 805 10SC 0 050 10 0 1 60 805 10WSC 0 050 10 0 1 60 805 SC Standard self centering piston en oe bes ie i z y Ti Titanium liquid contact parts 25SC 0 125 25 04 28 806 W Washing compartment for 50SC 0 250 E 50 0 5 E 14 806 salt concentrated solutions 100SC 0 500 100 05 7 807 a 200WTi 1 000 200 0 5 3 5 807 Pressure measurement and pulse dampening lt 1 ejeq SOUBWOJIag pue sbuey Bumuom Appendix E 3 Technical Data Appendix E Control and Interfaces Appendix E 4 Control and Interfaces External control A Gilson 305 321 331 or 333 Master pump or a PC with Gilson HPLC system controller software Externally controlled operating modes Constant flow rate Flow constant volume Dis pense and time based sequence Program with parameters identical to those of the 305 Master pump User interface Prime button Three lights indicating power on remote control and prime Rear panel microswitches for GSIOC identity number and baud rate selection Digital interface Gilson Serial Input Output Channel GSIOC slave connector on rear panel Electrical interface Manometric module connector on rear panel Technical Data Appendix E Environmental Conditions 3 N El Operati
15. 0000000000000 Fr CUI OO OOODOO8BOOOOO Eae CUI OOS OOOCOOS8BOOOOOO Emos OOO ODO BOO OOOO OOO METHYLETHYLKETONE TOO OOOOOOOOOOOOOO00 OCTANE CI OOO BOOOODOSBBOOOOOOBOO PENAE CJ DOO OOOOO8BOOOOOoO8ooo ISOPROPANOL TOO OOOOODODOODOOUOOOOOOOO D PROPYLE THER OOGO0 OD OO 00 B BIO 00000000 00 TETRAHYDROFURAN IO OO DO O0 Oo Oo O0 UoUJO UUJO OO OOOO OO DJ POWRNE QO OOO OOOOOOOOOOOOoOoOoOoOooo wer JODIO AAA O O O O O eee O OA mE TOO OOOOODOSBOOOOOOOOOOoOoOoOoO8o w a 3 all y BE w ea y 6 AER CEIGIGE Salle aula lia Salas ERASE ess O sas 922 Sella tarea Elsa 184 88 El Ml ivvscieie Appendix C 1 Solvent Miscibility Appendix C Appendix C 2 1st Generation i Pumping Systems Appendix D The following describes using a 306 with Gilson s first generation pumping systems Appendix D 1 1st Generation Pumping Systems Appendix D 802 3 4 Pressure signal Manometric Adapter Pressure signal 306 Pump Using a 306 with an 802 Manometric Module Appendix D 2 Using a 306 with an 802 Manomet ric Module To have the specified flow rate accuracy associated with the 306 pump the system controller for the 306 pump must receive a pressure signal When using an 805 806 or 807 type manometric module the output from the manometric module can be connected directly to the 306 pump When using an 802 803 or 804 manometric module the output can NOT be connected directly to the 306 pump It
16. 06 is designed for multi pump applications The 306 is a slave pump in Gilson chromatography systems and is controlled either by a computer with Gilson software or by a Gilson 305 Master pump acting as a system controller Introduction Using this Manual Using this Manual The 306 piston pump is a precision instrument which is simple and easy to use To gain the maximum from the instrument you should e Read the description of the instrument in chapter 2 e Install the instrument as shown in chapter 3 e Follow the operating instructions given in chapter 4 Introduction Unpacking The 306 piston pump is packed in a single carton Upon receipt of your instrument carefully unpack the unit and inspect it for possible damage This should be done immediately Check the contents of the carton against the parts list to verify that all parts are included and undamaged The parts list is given in Appendix A Do this now even if the unit will not be used immediately Report any damage to the responsible carrier immediately Read the description in chapter 2 to become familiar with the instrument its different parts and their names h Bunedun Introduction 1 4 Description 2 This chapter describes the physical layout of the 306 piston pump It describes the main body of the 306 and the position of the electrical connectors on the rear panel 2 1 Front View Description Front view The figure below shows a front view
17. 306 Pump User s Guide 306 Pump User s Guide LT801175G 2002 Gilson SAS All rights reserved September 2002 Table of Contents Satety 1 Introduction Usngtais mada nO 1 2 O na 1 3 2 Description E LOVE eee 2 2 A REF ene 2 3 3 Installation Elec calllNstal AOL PPP AA 3 2 MEU R EES 3 2 Seng he Voltage een A E 3 2 Mecha lla o A 3 4 Bumpihleschlinstallauo A 3 4 Mela aora 3 4 KNO eE CINE NS ee eee a e A 3 6 OEO NENE ES cceccecceccecerceco ccecnececoneenececocomnineceoceccnereccontecce 3 7 Manta LES y AA PA 37 AUtO ANaly caliSyStenmereekeneteeeeneeeeraseereereeserergeneesenetenseeesesee 37 Auto Preparative System a 3 9 Electrical CONE a 3 10 Connecting the Manometric Module 3 10 Connecting the 306 to Other Gilson Modules 3 11 Setting the GSIOC Identity Number of the 306 3 11 4 Operation Ariane eun EA espro nro 4 2 Checking that the 306 is Properly Connected 4 3 5 Maintenance and Troubleshooting BumpikleaeliN alntenanee se 52 IREI S EE One are E A 5 3 Eae NE S oa o tenes itis 5 3 Hydraulic Prop em ee 5 3 Appendices Appendix A Accessory Parts A SS E AA EE E AD A E OOC C OO ee Appendix C Sove Y Appendix D Ist Generation Pumping Systems nnn NISEN AMEN CA sere terres Table of Contents 1 Safety Read this section carefully before installing and operating the pump For safe and correct use of the pump it is essen
18. OC Interface Setting the GSIOC identity number of the 306 Every module connected to the GSIOC channel is identified by a GSIOC identity number Each module must have a different identity number If the system controller isa computer using a Gilson HPLC software package the identity number for each 306 slave pump can be set between 0 and 63 If the system controller is a Gilson 305 Master pump the GSIOC identity numbers for each slave pump must be as given in the table If it is a 321 or 33X Pump refer to the relevant pump user s guide Slave pump I D Number B 2 C 3 4 oO 90IS SUONIIVUOI 29111993 Electrical Connections GSIOC Installation 12345 OFF A o TES MA i ON GSIOC identity 0 6 Ol 12345 OFF E E jo ON GSIOC identity 1 12345 6 Ol GSIOC identity 2 OFF E jo ON 1234556 A E jo oF a eee MMM GSIOC identity 3 12345678 OFF 0000o IL GSIOC identity 4 The 306 GSIOC identity number is set using the switches on the rear panel Theidentity number is set according to the position of the switches Switches 1to 6 represent a binary number with position 1 being the least significant bit The 306 is shipped with the GSIOC identity number preset to 1 The figures opposite show the switch settings for GSIOC identity numbers 0 1 2 3 and 4 Switches 7 and 8 represent the GSIOC baud rat
19. and one 305 M aster pump see opposite or three 306 slave pumps controlled by a computer see below Collector Als 306 pump A 306 pump B 306 amp Inj That Computer OO sa npoyy ey Hbuluonisod 3 9 Installation Electrical Connections GSIOC 3 10 Electrical Connections GSIOC After positioning all of the modules in the system it is necessary to connect each slave pump to the system controller Two types of electrical connection must be made e The manometric module must be connected to one of the pumps in the system e All of the slave pumps must be connected to a system controller using the GSIOC cables provided Connecting the Manometric Module The manometric module has two functions to dampen the pulsations of the pump and to supply the current pressure value to the system controller The system controller needs this information to implement the flow rate accuracy corrections and to ensurethat the system pressure is not above or below the control limits entered in the program There are two possible ways of connecting the manometric module depending on the type of controller A Gilson 305 Master piston pump is the system controller In this case the output from the manometric module must be connected to the MANOMETRIC MODULE socket on the nearest pump whatever model it is 305 or 306 A computer with a Gilson software package is the system controller In this case
20. butor You must only use the type of fuse described and specified in this document 2 0 Amp type T slow blow for use where the power supply is between 100 V and 120V 1 0 Amp type T slow blow fuse for use where the power supply is between 220 V and 240V Safety 1 Safety However adequate protection including clothing and ventilation must be provided if dangerous liquids are used In case of inddental spillage carefully wipe with a dry doth taking into account the nature of the spilled liquid and the necessary safety precautions Cleaning installation dismantling maintenance adjustment and repair should only be performed by personne trained in such work and who are aware of the possible dangers involved This instrument must not be sterilized using an autoclave or any other mechanical device When you need to clean Symbol Explanation this instrument use one of the three following methods 1 a dean dry cloth PROTECTIVE CONDUCTOR 2 a loth dampened with water TERMINAL 3 a cloth dampened with soapy water Alternating current On Supply switch f If acloth dampened with soapy water is used to Off Supply switch clean the pump only domestic soap may be used Caton riskar Slecihesheck No other form of detergent or chemical may be used Caution refer to User s Guide These electronic and hazard symbols appear on the pump gt gt 0 Safety 2 Introduction The Model 3
21. e Switches 7 and 8 are shipped in the off position and should not normally be altered The baud rate can be changed to 9600 or 19200 9600 is represented by switch 7 off and switch 8 on 19200 is represented by switch 7 on and switch 8 off Operation 4 Connect the power cord to the 306 The power cord must be connected to a power socket that is grounded A grounded connection is necessary for safety and for the correct operation of the 306 pump To ensure correct operation all modules connected to the 306 should be connected to the same power line Switch on the 306 using the On Off switch on the rear panel The power indicator on the front panel should light If it does not light check that all of the electrical installation instructions have been correctly followed 4 1 Operation Priming the Pump Head Syringe Inject position de Run position Priming the Pump Head Do not run the pump when the pump head is dry This can result in severe pump head damage Check that the solvent bottle is filled with HPLC grade degassed solvent or buffer Immerse the inlet tubing filter into the solvent reservoir For 5SC 10SC 10WSC and 10WTi pump heads use the syringe supplied with the pump head to prime the pump as follows e Attach the syringe to the luer fitting of the low pressure prime valve refer to the figure below e Draw liquid into the syringe with the low pressure prime valve in the SYRINGE LOAD position
22. e suggested layouts are given below These layouts have been desigend to make the hydraulic and electrical connections as simple as possible Manual Injection System This is a binary gradient system with two pumps one manometric module one mixer and one detector The system controller is a Gilson 305 Master pump The different modules should be located as shown in the figure opposite The detector is located at the bottom of the stack and the pumps and other modules are positioned over it The 305 Master pump should be the top pump This makes it easy to read the display and to usethe keypad Auto Analytical System This is a binary gradient system with two pumps one manometric module one mixer one detector and one auto sampler Computer oO s jnpo ey Huluoljisod 3 7 Installation Positioning the Modules 3 8 Depending on the type of automatic sample injector used the modules are positioned in one of two ways The system can be either two 306 slave pumps and a computer see above or one 306 slave pump and one Gilson 305 M aster pump see below Installation Auto Preparative System This is a binary gradient system with two elution pumps one injection pump one manometric module one mixer one detector and one fraction collector In this configuration automatic injection is performed by the injection pump located below the elution pumps The system can be either two 306 slave pumps
23. efer to User s Guide for the pump head Check that the pump head is properly mounted Air bubbles appear in both inlet and outlet tubing Loose connection of inlet tubing Worn flange of inlet tubing Inlet filter partly clogged Refill time is too long for the solvent Tighten the connection but do not overtighten Replace the inlet tubing Clean or replace the inlet filter Decrease the refill time Air bubbles appear only in outlet tubing Loose connection of outlet tubing Tighten the connection but do not overtighten al BunNooysajqnoAL 5 3 Maintenance and Troubleshooting 5 4 Accessory Parts List Appendix A Parts lists for the 306 Pump consisting of Standard Accessories and Additional Accessories Appendix A 1 Accessory Parts List Appendix A Reference Standard Accessory Parts List Description 3645388 36610101 6730204007 7080316105 6730104006 7080316106 LT801175 Standard Accessory Parts List Appendix A 2 SC type pump head clamp Double ended wrench 1 4 5 16 Fuses 2 0 Amp type T slow blow 5 x 20 mm for 100 120 V Power cord for 100 120 V Fuses 1 0 Amp type T slow blow 5 x 20 mm for 220 240 V Power cord for 220 240 V Model 306 User s Guide Accessory Parts List Appendix A Additional Accessory Parts List Reference Description 36078143 GSIOC cable 03434939 Mast clamp 2105703 Hex Mast 3 4 x 16
24. he Gilson HPLC system controller software RS232C cable GSIOC cable GSIOC slave GSIOC slave Computer Gilson RS232C GSIOC interface Slave Devices Appendix B 2 GSIOC Control Appendix B GSIOC commands Non specific commands The 306 software includes a set of commands that offers direct compatibility with first generation Gilson pumps Command Type Function Cornos 0 U0UU UU N Q A Request Status Begin Dispense Cycle s Set Dispense Mode Set Flow Mode Lock Pump Stop Flow Unlock Pump Master Reset All commands are detailed below with their type mode and function Immediate commands have their response format described Buffered command parameters are documented Comments are added where necessary spuewuwo DOISD Immediate Request Status Response format abcdefgh where a is the error status L for low pressure limit H for high pressure limit T for Invalid settings gt blank for no error bp is the control status L for locked and U for unlocked cdefg being the current parameters and h the mode status F for Flow mode M for Microflow mode D for Dispense mode S for Stop not in dispense mode Buffered B Begin Dispense Cycle s Syntax Bn Parameter n is the number of dispense cycles If n is omitted it is considered as 1 by default Buffered D
25. mm 1517 SUed 1OSSIDIY jeuonippy Appendix A 3 Accessory Parts List Appendix A Appendix A 4 GSIOC Control Appendix B This chapter explains how to control the 306 from a computer using Gilson HPLC system controller software Appendix B 1 GSIOC Features GSIOC Control Appendix B GSIOC Features GSIOC stands for Gilson Serial Input Output Channel This communications channel links all of the Gilson modules in a system together The system controller controls all of the modules in a system by sending GSIOC commands to the slave modules for example pumps or detectors Each device connected to the GSIOC channel is distinguished by a GSIOC identity number between 0 and 63 The GSIOC identity number is set by switches inside each module or by the module s software The controller communicates with one slave device at a time The hardware and software requirements to control a module from a computer using the GSIOC are as follows e A PC running under Windows 5 98 or NT e A Gilson interface module 506C e A Gilson HPLC system controller software package For more details consult the To get started you must documentation that accompanies the Gilson e Install the Gilson HPLC system controller software software on the computer e Connect the computer to the Gilson interface using the cable provided with the interface e Connect the output from the interface to the Gilson module using a GSIOC cable e Start t
26. ng Conditions gt Indoor in altitudes up to 2000 m 4 to 40 C and in D humidity up to 80 o O Size Weight W x Hx D 33 x 15 x 33 cm 13 x 6 x 13 inches 10 kg 22 pounds with pump head Voltage Power Frequency 100 120 V or 220 240 V 120 VA 50 60 Hz Appendix E 5 Technical Data Appendix E Appendix E 6 World Wide Web wwwgilson com E mail sales gilson com service gilson com training gilson com World Headquarters Gilson Inc 3000 W Beltline Hwy PO Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Be Gilson SAS 19 avenue des Entrepreneurs B P 145 95400 Villiers le Bel France Telephone 33 1 34 29 50 00 Fax 33 1 34 29 50 20
27. olume x 10000 pump head size In our example xxxxxxx 2ml x 10000 4000 5 The GSIOC command is v4000 For the dispense flow rate you send the command Axxxxx Calculate xxxxx Flow rate x 10000 pump head size In our example xxxxx 5ml min x 10000 100000 5 The GSIOC command is d10000 spuewWwo DOISD Appendix B 7 GSIOC Control Appendix B GSIOC Commands Appendix B 8 The complete sequence is 1 Buffered L 2 Buffered D 3 Buffered v4000 4 Buffered d10000 5 Buffered B1 These steps can be sent as individual GSIOC commands to the 306 pump or they can be sent as one instruction To send all of these commands as one instruction send LDv4000d10000 The command to start the injection pump is B1 This command is sent when you want to start the pump Solvent Miscibility Appendix C Miscibility means that solvents should mix with each other in all proportions That solvents should be miscible is important both during elution and when switching from one solvent to another You are advised to refer to the table below when selecting solvents For some solvents lower toxicity alternatives are indicated 1 2 3 as follows encan ACETONE E i 1 Dichloromethane O00000 Bice aia Cao OOOO DCHLORCETHANE O DOOOOOO DICHLOROMETHANE G DMETHYLFORMAMDE J OO OO OO OO DEMSUFXE TOO OOBOOOO Dome CJ OOO OOBOOOOO Emane OOOO OOOOOOOO EmMG O000000000000 DFETHYLETHER OQO
28. or 303 which it is not able to deliver the slave sends a warning to the controller and the message Invalid settings is displayed The motor runs at its maximum speed but the flow rate will not be accurate The maximum flow rate that you can program depends on the pressure in the system and the compressibility of the liquid that you are using 600 600 280 140 70 35 0 z0 e yum gos e Buisn Appendix D 3 1st Generation Pumping Systems Appendix D Appendix D 4 Technical Data Appendix E The following information presents construction and operational characteristics for Gilson 306 Pumps Appendix E 1 Technical Data Appendix E Type of Pump Appendix E 2 Type of Pump Externally controlled reciprocating pump with single piston interchangeable head constant stroke and fast refill motion Installation category II and pollution degree 2 Technical Data Appendix E Working Range amp Performance Data Working Range Pump Heads and associated Manometric Modules A complete Gilson liquid delivery system includes up to four pumps a manometric module and a mixer Liquid contact materials 316L stainless steel titanium sapphire ceramic ruby PCTFE PTFE HDPE Flow rate precision and accuracy at 20 C over full working range Coefficient of variation 0 1 0 6 with aqueous solutions or hydro organic polar solvent mixtures and 0 3 1 with hydrocar bons or chlorinated
29. tial that both operating and service personnel follow generally accepted safety procedures as well as the safety instructions given in this document the 306 Pump User s Guide The instrument described in this document is a slave piston pump for use in multi pump applications It can be controlled either by a computer with Gilson software or by a Gilson 305 Master pump acting as a system controller It should only be used in the laboratory or similar indoor environment by qualified personnel If the instrument is used in a manner not specified by Gilson the protection provided by the instrument may be impaired Ensure that the ventilation fan on the Piston Pump operates and is not obstructed when the instrument is installed Voltages present inside the instrument are potentially dangerous If thereis a problem with theinstrument the power cable should be removed until qualified service personnel have repaired it This is to prevent anyone from inadvertently using the instrument thus causing possible harm to themselves or damage to the instrument itself The leakage current of this instrument is within the limits allowed by safety standards for laboratory equipment An efficient ground connection is imperative for the physical protection of the user Power supply cord reference 7080316106 is for use in France and Germany Power supply cord reference 7080316105 is for use in USA and Canada For other countries contact your local Gilson distri
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