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Peggy User Guide

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1. Open Source Software Certain items of independent third party code may be included in the Licensed Software that are subject to open source licenses Open Source Software Such Open Source Software is licensed under the terms of the license that accompanies such Open Source Software Nothing in this Agreement limits Customer s rights under or grants Customer rights that supersede the terms and conditions of any applicable end user license for such Open Source Software In particular nothing in this Agreement restricts Customer s right to copy modify and distribute such Open Source Software that is subject to the terms of such open source licenses Ownership Company reserves all rights not expressly granted to Customer in this Agreement Without limiting the generality of the foregoing Customer acknowledges and agrees that except as expressly set forth in this Agreement Company and its suppliers retain all Intellectual Property Rights title and interest in and to the Licensed Software and Documentation Services Subject to Customer s payment of the Services fees as set forth in the System Quotation and to the terms and conditions herein Company will use commercially reasonable efforts to provide to Customer the following support and maintenance services the Services for the Licensed Software a Company will answer technical questions concerning functions and features of the Licensed Software b Company will provide
2. Preparation for Storage or Shipment Storage ProteinSimple recommends storing the instrument if it will not be used for two weeks or longer To prepare the system for storage Peggy User Guide page 78 Chapter 5 Maintenance and Troubleshooting 1 Open the resource tray and remove any remaining capillaries along with the Wash Running and Matrix Removal Buffer cups Discard any unused wash running and matrix removal buffers Rinse the bottles with 0 22 micron filtered deionized water molecular biology grade or better and let air dry When dry cap and store at room temperature NOTE Failure to remove and cap reagents may lead to evaporation and unnecessary concentration of reagents which will affect assay performance 2 Open the sample tray and remove the sample plate NOTE Regardless of reagent levels replace all assay reagents with fresh solutions every 24 hours 3 If hazardous samples have been analyzed on Peggy perform the Post Run Decontamination on page 76 procedure 4 Empty the waste bottle and reinstall Shipment Preparation To prepare Peggy for shipment 1 Perform Instrument Cleaning on page 75 2 Perform Post Run Decontamination on page 76 3 Empty the waste bottle and reinstall Spare Parts For assistance with replacement parts please contact ProteinSimple Technical Support at 888 607 9692 Description Part Number Auxiliary Module Waste Bottle 002 6
3. IWARNING CHEMICAL HAZARD Some chemicals used can be potentially hazardous and can cause injury or illness Read and understand the Material Safety Data Sheets MSDSs provided by the chemical manufac turer before you store handle or work with any chemicals or hazardous materials Minimize contact with and inhalation of chemicals Wear appropriate personal protective equipment when handling chemicals e g safety glasses gloves or clothing For additional safety guidelines consult the MSDS Do not leave chemical containers open Check regularly for chemical leaks or spills If a leak or spill occurs follow the manufacturer s cleanup procedures as recommended on the MSDS Comply with all local state provincial or national laws and regulations related to chemical storage handling and disposal Peggy User Guide Safety page 5 Chemical Waste Hazards IWARNING BIOHAZARD A Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen ta
4. Primary Ab gt Secondary Ab Quantitate Signal Peggy User Guide Native protein lysate samples and assay reagents are prepared loaded into an assay plate and placed in Peggy Peggy loads samples into the capillary automatically Proteins and ampholytes migrate through a separation matrix are separated by charge and resolve according to their expected pl values The separated proteins are then immobilized to the capillary wall via a proprietary UV capture method Target proteins are immuno probed using a primary antibody and detected using an HRP conjugated secondary antibody and chemilu minescent substrate The resulting chemiluminescent signal is detected and quantitated Analysis is auto matically performed and quantitative results including pl value and signal intensity are presented in Compass software page 4 Chapter 1 General Information Safety User Attention Notifications Several user attention phrases are used throughout this manual Each phrase should draw the following level of attention from the user NOTE Points out useful information IMPORTANT Indicates information necessary for proper instrument operation CAUTION Cautions users regarding potentially hazardous situations in regard to user injury or dam age to the instrument if the information is not heeded IWARNING Warns users that serious physical injury can result if warning precautions are not heeded Chemical Hazards
5. 0 200 mL Primary Antibodies in Antibody Diluent 0 180 mL Streptavidin HRP 0 010 mL Secondary HRP Conjugate 0 180 mL Luminol Peroxide Mix 0 180 mL Prepared Sample contains Sample Buffer DTT Fluorescent Standards and Water 0 055 mL Assumes 1 row of sample and 1 row of antibody Peggy User Guide Chapter 1 General Information Safety Waste Origin Charge based Assays Volume Trough Reagents for 1 cycle Anolyte 10 mM 1 0 mL Catholyte 100 mM 1 0 mL Wash Buffer 3 0 mL Water for trough and manifold cleaning 80 0 mL Total waste per cycle 85 0 mL Assay Plate Reagents Luminol Peroxide Solution 0 025 mL Antibodies in Antibody Diluent 0 075 mL Example Cell Lysate Sample Cell lysate Sample Diluent Premix Protease inhibitors Phosphatase inhibitors Labeled peptides 0 036 mL Assumes row of sample and 1 row of antibody Peggy User Guide page 7 page 8 Chapter 1 General Information Material Safety Data Sheets Some chemicals used with Peggy may be listed as hazardous Warnings are displayed on the labels of all chemicals when hazards exist MSDSs provide users with safety information needed to store handle transport and dispose of the chemi cals safely ProteinSimple recommends updating laboratory MSDS records periodically Material Safety Data Sheets for ProteinSimple reagents are available online at www proteinsimple com liter ature or by calling 888 607
6. 9692 Otherwise call the chemical manufacturer directly or visit their web site Instrument Safety Labels The following safety labels are located on Peggy Each label will display a safety alert symbol indicating a potential safety hazard Symbol Description Risk of Electric Shock Refer to Peggy User Guide before proceeding Danger of hazardous waste Use caution in these areas This warning only applies if using hazardous material Peggy reagents are not considered hazardous waste If you are using hazardous materials please contact your field service representative to place labels in the appropriate locations gt gt gt WARNING If the unit is not used as specified by ProteinSimple the overall safety will be impaired WARNING If the unit is damaged and does not function properly stop the unit safely and contact ProteinSim ple Technical Support immediately WARNING No user replaceable serviceable parts except for the ionizer fan and separation block Peggy User Guide Safety page 9 Sharps Hazard IWARNING SHARPS HAZARD Capillaries may present a potential sharps hazard Dispose of used capillaries in biomedical waste sharps containers Pinch Hazard WARNING PINCH HAZARD Do not remove the transparent front window on Peggy unless instructed to do so by ProteinSimple Technical Support personnel The window protects users from a potential pinch hazard that could be caused
7. BY THIS AGREEMENT AND 2 YOU REPRESENT THAT YOU HAVE THE AUTHORITY TO ENTER INTO THIS AGREEMENT PERSONALLY OR IF YOU HAVE NAMED A COMPANY AS CUSTOMER ON BEHALF OF THAT COMPANY YOU OR ANY SUCH COMPANY THE CUSTOMER AND TO BIND THE CUSTOMER TO THE TERMS OF THIS AGREEMENT IF YOU DO NOT AGREE TO ALL TERMS AND CONDITIONS OF THIS AGREEMENT OR IF YOU DO NOT HAVE SUCH AUTHORITY YOU SHOULD CLICK ON THE CANCEL BUTTON TO DISCONTINUE THE DOWNLOAD OF THE LICENSED SOFTWARE 1 Definitions 1 1 Authorized Use Parameters means the following usage restrictions which restrict the operation of the Licensed Software to a particular set of conditions Customer shall a limit simultaneous use of the Licensed Software to a maximum of ten 10 Authorized Users and b use the Licensed Software only in connection with the accompanying System purchased by Customer pursuant to the System Quotation and located at the Site 1 2 Authorized User means one 1 User who initiates the execution of the Licensed Software and or interacts with or directs the Licensed Software in the performance of its functions Multiple Authorized Users may work simultaneously with one installation of the Licensed Software as on a server or they may each have their own installation on single user machines or a mix of these provided that in all cases the total number of simultaneous Users does not exceed the applicable Authorized Use Parameters 3 Company means
8. Help in the main menu and click Check for Updates Maintenance Daily Empty the Capillary Discard Tray Empty the capillary discard tray on a daily basis after each run The discard tray is located under Peggy s sys tem tray Monthly lonizer Cleaning Items needed Isopropyl alcohol cleaning swab lint and fiber free VWR P N 89133 806 and the screwdriver P N 040 994 provided in the Peggy accessory kit 1 Open Peggy s right door to gain access to the ionizing fans 2 Remove the thumbscrew holding the protective cover over the fans Peggy User Guide Maintenance page 73 Thumbscrew 3 Using the cleaning swab rub isopropyl alcohol over each of the 4 ionizing pins on the fan to remove any build up Pins 4 Replace the panel and captive screw Peggy User Guide page 74 Chapter 5 Maintenance and Troubleshooting Yearly ProteinSimple recommends that an annual preventive maintenance visit be performed by an authorized ProteinSimple representative Please contact Customer Support to schedule a visit Leveling the Instrument This option allows the user to see if Peggy is properly leveled Select Instrument and click Leveling to access this option The following screen displays the level status of the instrument Leveling xs After leveling adjust the sensor reading to 0 Instrument level sensor should read between 0 05 to 0 05 NOTE If the reading is not between 0 05 a
9. Peggy includes a computer to run the instrument and analyze resulting data using Compass software The software is preinstalled A CD containing Compass software is also provided with Peggy For post run data analysis Compass software can also be installed on a separate workstation such as a user s desktop com puter This computer must meet the minimum requirements listed in the table below in order to run Com pass software and process data Component Minimum Recommended Operating System Windows XP Windows 7 or Vista Processor Core 2 Duo Memory 2 GB Free Disk Space 10GB Table 2 5 Computer requirements General Guidelines and Information System Operation Notification of Intended Use NOTE Peggy is for research use only Not for use in diagnostic procedures Lifting and Moving the System Use Proper Lifting Precautions IMPORTANT Use caution when lifting or moving Peggy The system weight is 82 kg 180 Ibs Two or more peo ple are required to lift the system onto the laboratory bench Proper lifting safety precautions must be taken Peggy User Guide page 20 Chapter 2 Lab Requirements Peggy User Guide page 21 Chapter 3 Peggy Chapter Overview Instrument Overview Auxiliary Module Compliance Safety Guidelines Physical Specifications Peggy User Guide page 22 Chapter 3 Peggy Instrument Overview Peggy s individual hardware components are described in this section A
10. block contains two troughs which will be automatically filled with buffer s by the instrument Peggy will also remove buffer s and wash the troughs after completion of the separation step Chemiluminescent and fluo rescent CCD images are taken of the separation in the capillaries through the detection window The tray holds 12 capillaries Size based assays both the front and rear troughs are used for Running Buffer Charge based assays the front trough is used for Anolyte and the rear trough for Catholyte Rear Trough Separation Block Detection Window Front Trough Peggy User Guide Instrument Overview page 33 Capillary Discard Tray The capillary discard tray is used to dispose of capillaries as the execution of each cycle completes Protein Simple recommends placing an inverted plate lid in the tray area to contain capillaries as well as emptying the lid on a daily basis Discarded Capillaries Plate Lid WARNING SHARPS HAZARD Capillaries may present a potential sharps hazard Dispose of used capillaries in biomedical waste sharps containers NOTE An overfilled capillary discard tray may cause jamming of the sample tray If this occurs call techni cal support at 888 607 9692 or 408 510 5500 option 3 Peggy User Guide page 34 Chapter 3 Peggy Manifold Wash Station The manifold wash station is used to wash and clean the instrument manifold after each separation and to prevent build
11. by the moving robot and gripper assembly Door Interlock A door interlock is engaged when a run is started to prevent users from opening the doors during a run This eliminates any dangerous user interactions with the robot as well as exposure to high voltage and UV light A yellow indicator light indicates when the instrument is running and the interlock is engaged When the system status is READY and the doors are open the robot s XZ stage motors high voltage power supply and UV power supply are disabled WARNING Do not override the door interlock The interlock protects users from exposure to UV light and a potential pinch hazard that could be caused by the moving system tray System Tray CAUTION Do not attempt to open or close the tray manually Instead use the Open Tray command from the Instrument menu in Compass software Peggy User Guide page 10 Chapter 1 General Information Prior to System Operation Ensure that all users of Peggy have Received instruction in general safety practices for laboratories Received instruction in specific safety practices for the instrument Received instruction on handling of biohazards if biohazardous materials are to be used on the sys tem Read and understood all related MSDSs CAUTION Avoid using Peggy in a manner not specified by ProteinSimple While the system has been designed to protect the user this protection may be impaired if the instrument is used imp
12. illary box in the designated resource tray position IMPORTANT Capillaries are light sensitive Keep the cover on the box until you are ready to transfer the capillary box to the resource tray Place assay plate into the sample tray of the instrument and press Start Step 2 Start the Run 1 You can start a run in one of two ways depending on what button is displayed in the status bar NEW RUN BUTTON click New Run Peggy User Guide Starting a Run Size based Assays page 47 a Select File in the main menu and click Open Assay pP Ready PI File Edit Instrument Window Help New Assay gt Open Assay gt Protein Sizing Protein Test Save Save s Besa ote Simple Western Demo Plate Import Protocol Simple Western Import Template Browse Export Protocol Export Template apes S Separat Print gt 5 Matrix Blockin Exit i Primary b A list of the last five assays opened will display Select one of these assays or click Browse to open the Assay folder and select a different assay c Alternatively choose New Assay and select Peggy Size to get the default Peggy assay conditions d The Start button will display START BUTTON this indicates an assay is already loaded a a Go to the Assay screen and verify this is the assay you want to use If not select File in the main menu click Open Assay and select another assay 2 Click Start This will launch the Start Run
13. of such courts Injunctive Relief Customer acknowledges that the Licensed Software contains valuable trade secrets and proprietary information of Company that any actual or threatened breach of this Agreement will cause harm to Company for which monetary damages would be an inadequate remedy and that injunctive relief is an appropriate remedy for such breach Modifications Company reserves the right to change the terms and conditions of this Agreement or its policies relating to the Licensed Software at any time Company will notify Customer of any material changes to this Agreement by sending Customer an e mail to the last e mail address Customer provided to Company or by prominently posting notice of the changes on Company s website Any material changes to this Agreement will be effective upon the earlier of thirty 30 calendar days following Company s dispatch of an e mail notice to Customer or thirty 30 calendar days following Company s posting of notice of the changes on Company s website These changes will be effective immediately for new users of our Licensed Software Please note that at all times Customer is responsible for providing Company with its most current e mail address In the event that the last e mail address that Customer has provided Company is not valid or for any reason Company is not capable of delivering to Customer the notice described above Company s dispatch of the e mail containing such notice will nonetheless con
14. perform a cleaning protocol to remove used capillaries from system trays This is required to prepare the system for the next run Click Cleanup Cleaning Cleaning ea Wed 3 48 PM Wed 3 56 PM us Allow Peggy to complete the cleaning protocol which takes about ten minutes When complete instru ment status will change to Ready and a new run can be started Waste and Water Bottles The waste bottle on the Auxiliary Module should be emptied before the initiation of every new run and the water bottle refilled To remove or install bottles IMPORTANT Do not attempt to remove a bottle while Peggy is executing a run This may abort the run in prog ress ee Po Remove the bottle by unscrewing it clockwise until it disengages from the manifold Pull the bottle down slowly until the sensor at the bottom of the bottle is clear Fill the water bottle up to the base of the neck or empty the waste bottle as needed To reinstall the bottle lift it up to the manifold making sure to enclose the sensor that sits at the bottom inside the bottle Screw the bottle counter clockwise until the arrow on the bottle s alignment label lines up with the arrow on the manifold s alignment label Peggy User Guide Controlling Peggy page 63 IWARNING BIOHAZARD A Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual
15. perform to support the Licensed Software Company may subcontract the Services to a third party contractor provided that Company will be responsible for the third party contractor s compliance with this Agreement Service Exclusions Company will not be obligated to provide the Services if a Company determines that an Error is caused by malfunction of any hardware other than malfunction of the System or third party software used with the Licensed Software or b Customer has failed to incorporate the latest Update previously released to Customer 4 Warranty 4 1 4 2 4 3 Licensed Software Warranty Company warrants that the Licensed Software as properly installed and under normal use will perform substantially in accordance with its Documentation during the Warranty Period The Warranty Period for the Licensed Software begins on date Customer downloads the Licensed Software and ends twelve 12 months thereafter Remedy If Customer notifies Company in writing during the Warranty Period of an Error Company will at its expense and as its sole obligation for any breach of the foregoing warranty use commercially reasonable efforts to correct the Error or replace the Licensed Software Any Error correction or replacement of the Licensed Software will not extend the original Warranty Period The warranty and the remedies provided above will not apply to the Licensed Software if a Company determines that an Error is caused by acci
16. the capillary automatically Proteins are separated by size as they migrate through both a stacking and separation matrix The separated Low MW proteins are then immobilized to the capillary wall via Load Sample 7 a proprietary UV capture method Target proteins are Separate l immunoprobed using a primary antibody and gt detected using an HRP conjugated secondary anti body and chemiluminescent substrate The resulting imr biliz chemiluminescent signal is detected and quantitated Analysis is automatically performed and quantitative results including molecular weight and signal inten I gt e A sity are presented in Compass software Immunoprobe KDD Dea 0 Y Target Protein HRP labeled Primary Ab Secondary Ab Quantitate NN er Signal Chemiluminescence 0 66 90 n 180 MW kDa Peggy User Guide Simple Western Assays on Peggy Charge Based Assays page 3 Simple Western charge assays let you do in depth characterization of proteins Because proteins are sepa rated by their charge or pl extremely small isoelectric point differences are detected letting you see dis creet changes in protein isoforms 06 00 0000 0 0 0 oe ea eT Load Sample e Bram e o o o o P TARN Low pl High pl ji Separate Immobilize g A l Immunoprobe 3 3 Y Target Protein Y HRP labeled
17. 11 Wrench Combination 1 2 5 8 Open End 040 992 Wrench Driver Allen M2 5 040 993 Peggy Instrument Cup Set 3 041 595 Waste Water bottle replacement kit 040 710 Peggy User Guide Troubleshooting page 79 Troubleshooting For instrument and assay troubleshooting information please contact ProteinSimple Technical Support at 888 607 9692 option 3 support proteinsimple com or visit http www proteinsimple com technical_ support html Peggy User Guide page 80 Chapter 5 Maintenance and Troubleshooting Peggy User Guide
18. Assembly Fluidic components in the head assembly execute all bulk reagent dispensing and sample tray reagent load ing steps Pheumatic components provide control for the gripper which is used to pick up and transfer cap illaries Gripper The gripper is located at the base of the head assembly and is used to transfer capillaries between system trays The gripper will pick up the 12 capillaries from the capillary clip and move them to and from each area in the tray during a cycle within a run The gripper rotates 90 degrees to allow vertical transfer and horizontal placement of capillaries X Z Stage Robot The X Z stage robot moves the head assembly to and from the sample resource incubator separation and capillary discard trays during run execution The robot also moves the pipettor which is used to transfer flu ids and perform washes within the instrument Peggy User Guide page 26 Chapter 3 Peggy Syringe Pump The syringe pump is used to pipette Dl water reagents in the resource tray cups and also assists in washing the manifold head as dictated by the assay protocol lonizer Fans Anti static ionizer fans are mounted on the inside of Peggy s right door These fans are used to circulate ion ized air inside the system This provides a uniform static charge that aids in proper capillary transfer during run execution Peggy User Guide Instrument Overview page 27 System Trays Peggy utilizes five compartmental tr
19. Depth 0 30 1 00 Height 0 51 1 67 Tubing between Auxiliary 3 00 9 84 Module and Instrument Computer Workstation Width 0 66 2 17 Depth 0 76 2 49 Height 0 41 1 35 Table 2 1 Peggy dimensions Dimension Meters Feet Width 2 13 6 98 Depth 0 91 2 99 Height 1 00 3 28 Table 2 2 Recommended space requirements for Peggy Peggy User Guide page 17 page 18 Chapter 2 Lab Requirements Electrical Requirements Peggy requires a dedicated grounded circuit capable of delivering the appropriate current and voltage for your country The power requirements for select countries are listed below Region Volts AC Frequency Hz Amps US and Canada 120 60 5 5 Europe 240 50 27 Japan 100 50 60 6 5 Table 2 3 Power requirements In addition to the requirements listed above ProteinSimple requires the grounded circuits terminate at the receptacles and receptacles must be located within 10 ft 3 m of the instrument Environmental Requirements Please provide appropriate heating and cooling to maintain a constant laboratory temperature For optimal performance the lab environment must meet the following criteria Requirement Specification Operating temperature range 18 24 C 64 75 F Operating humidity range 20 60 relative non condensing Table 2 4 Environmental requirements Peggy User Guide Software and Computer Requirements page 19 Software and Computer Requirements
20. ENI TTAVS EREA snakk 43 Auxiliary Module Reservoirs 43 Physical Specifications eei arn Terai a E 44 Chapter 4 Operating Peggy ss 45 P wer UDe riro e e AA ks ESSN AER aa 46 Starting a Run Size based Assays 46 EE E E E AT 46 Step 2 Start the RUN 0 0 cece O TaY 46 Step 3 Post RUN Procedures 53 page ii Starting a Run Charge based Assays 54 Step 1 Get RAG aei e IA AET 54 sep Stand a 54 Step 3 Post Run Procedures 61 StODDING A RUMA rE EEEE Swe SRG oes Clic 62 Waste and Water Bottles 00 eee e eee e ee 62 Controlling REgGY stranga ns 63 Starting a New Ra x 6 s 63 CHCGIING OAT IE E TETE ancient 64 Cleaning After a Run Error n 66 S fn E A 67 Viewing and Changing System Properties 68 Viewing Error and Test LOgS a se 68 Peggy s Status MOdES 0 ccc cece cece cece ees 68 SHULIOWN ix citer hes eee cist iin aneae eee 69 Chapter 5 Maintenance and Troubleshooting 71 Software UpdatEssi isis ceded AEE 72 MAINE NA 72 Annan 72 MONTAN sasssa Aas ees eee 72 YOGI EEIE EEEE 74 Instrument CEO er i ra EE Ik 75 Manifold Flash ss 75 System Decontamination 26 00 76 Preparation for Storage or Shipment 77 SOI JE ORG En SRR e a ei tein ne ae ee 77 Shipment Preparation 0 cece eens 78 Spare PONS sce nid thnks canta Peek EA He eee ees 78 POUDICS
21. Error verification analysis and corrective efforts for the Licensed Software and c Company will provide without charge Updates of the software released during the term of this Agreement Customer will be responsible for providing in a manner consistent with good industry practice all Services to Users Customer acknowledges that Company may not be able to correct all reported Errors Any Update of the Licensed Software will be deemed part of the Licensed Software and Customer will use such Updates in accordance with the requirements and obligations in this Agreement Service Conditions Company s obligation to provide the Services is conditioned on Customer a notifying Company of any Error within a reasonable period of time b providing Company all information relating to the Error c providing access to the Licensed Software and Customer s facility where the Licensed Software is located and informing Company of any potential hazards which may be encountered while servicing the Licensed Software Customer may contact Company via telephone at 1 888 607 9692 or e mail at Peggy User Guide Legal Notices page 13 3 3 support proteinsimple com during the hours of 8 a m Pacific Time and 5 p m Pacific Time Monday through Friday excluding holidays to report any Error A list of standard holidays will be provided to Customer upon request Company shall have the right to determine in its sole discretion what corrective action Company will
22. MOOUING 2g EEEE E E i 79 Peggy User Guide page 1 Chapter 1 General Information Chapter Overview Welcome Simple Western Assays on Peggy Safety Customer Service and Technical Support Legal Notices Peggy User Guide page 2 Chapter 1 General Information Welcome Congratulations on bringing Peggy into your lab ProteinSimple welcomes you as a new user This user guide will cover important safety and instrument information installation requirements maintenance and operating instructions Simple Western Assays on Peggy Peggy performs Simple Western assays two ways letting you separate proteins either by size or by charge Whatever you decide all you have to do is load your samples in Peggy and press start Simple Westerns take place in a capillary Peggy automates all steps of the process for you including sample loading protein separation immunoprobing washing detection and data analysis Up to 12 samples can be analyzed simultaneously in a single cycle and up to 8 cycles 96 samples can be processed in a single run Size Based Assays A Simple Western size assay is an automated Western no gels no transfer devices no blots no film and no manual analysis Samples are first treated with SDS and DTT and then Load Matrix f heat denatured Assay reagents and prepared samples are loaded into an assay plate and placed in Peggy Stacking Matrix O Separation Matrix Peggy loads samples into
23. ProteinSimple 4 Documentation means Company s then current manuals guides and on line help pages if any applicable to the Licensed Software and made generally available by Company to its customers 5 Enterprise means those organizations that have Internet addresses located at top level and second level domain names set forth in the System Quotation 6 Error means a reproducible error in the Licensed Software that prevents such Licensed Software from operating substantially in accordance with its Documentation 7 Executable Code means the fully compiled binary version of Licensed Software that can be executed by a computer and used by an end user without further compilation 8 Intellectual Property Rights means all copyrights trade secrets patents patent applications moral rights contract rights and other proprietary rights but specifically excluding any trademarks or service marks 9 Licensed Software means the Compass software program in Executable Code form and any Updates that Company makes available to Customer in accordance with this Agreement Peggy User Guide page 12 Chapter 1 General Information 1 10 Site means the facility or campus set forth in the System Quotation 1 11 System means the proprietary NP1000 NP100 Simon Sally or Peggy protein analysis system or any future model or successor thereto that is provided to Customer by Company pursuant to a separ
24. Running Buffer Matrix Removal Buffer Starting a Run Size based Assays page 51 6 Page 4 Load Sample Plate The sample tray will automatically open Place the lidded 384 well plate on the cooling block in the tray ensuring that the AT well position is aligned with the upper left corner of the cold block When this is complete click Next The sample tray will close A1 Position Peggy User Guide Start Run Load Sample Plate Remove the old sample plate and load the new plate 12345678 90NLHUHETBHMADDM A B c D E F G H 1 J K L M N o P vVOZLErXC IONMmDOU gt page 52 Chapter 4 Operating Peggy NOTES Peggy requires that plate lids be used on sample plates Ifa lid is not detected a message will be displayed in the Start Run Wizard Compass software will reopen the sample tray to allow the user to insert a lid When inserting the sample plate ensure that the plate is firmly seated and level on the cold block Plates that are not level can interfere with the movement of the sample tray 7 Page 5 Data File The data file name will automatically default to the assay name appended with the current date and time To change the file name begin typing in the text box To change the directory where the data file will be stored click Browse Start Run z J Begin the Automated Run The following protocol will be run You can change the results location and prefix As
25. Wizard Ready NOTE If the manifold was not cleaned prior to starting the run a message indicating this will display If this occurs click Yes to cancel the run and perform the manifold cleaning Peggy User Guide page 48 3 Chapter 4 Operating Peggy Page 1 Check Water and Waste The fluid levels in the accessory module bottles will be checked by the software If the levels in both bottles will allow Peggy to complete the run the wizard screen will dis play Water Level OK and Waste Level OK messages Click Next to proceed Start Run a Check water and waste Make sure there is enough water and room in the waste bottle LL Waste Water The run will be terminated if the water bottle empties or the waste bottle fills during the run Water level OK Waste level OK lt Back Next gt Start Cancel NOTE If the waste level is too high or the water level is too low to complete the run messages to indicate one or both will be presented in this screen If this occurs fill or empty each bottle as indicated using the procedures outlined in the Removing and Installing Bottles on page 39 When this is complete the error status will be automatically updated and allow the Start Run Wizard to proceed Peggy User Guide Starting a Run Size based Assays page 49 4 Page 2 Replace Sponge A new sponge should be used each time a new experimental run is started Disc
26. ard the old sponge and put a new sponge in the manifold wash station Start Run Replace Sponge Put a new sponge in the manifold wash station 5 Page 3 Start The resource tray will automatically open Fill the Wash Buffer Running Buffer and Matrix Removal Buffer cups and insert the capillary box in the primary capillary box position Add a secondary capillary box if more capillaries are required Click Next when finished The resource tray will close NOTE Make sure enough capillaries are loaded to complete the run A capillary box should be inserted in the primary position first and if additional capillaries are needed use the secondary position When the primary box is depleted Peggy will automatically move to the secondary box Discard leftover Running Buffer Wash Buffer and Matrix Removal Buffer prior to refilling bottles for new run Peggy User Guide page 50 Hinar E Wn Start Set up the instrument to start a new run Load the Wash Running and Matrix Removal buffers Wash Running Matrix Removal Load a primary cap box Add a secondary cap box if more capillaries required Primary Cap Box Secondary Cap Box lt Back Net gt Sta Cancel Chapter 4 Operating Peggy NOTE You an also refer to the labels on the resource tray for proper insertion of reagents Primary Capillary Box Secondary Capillary Box Peggy User Guide Wash Buffer
27. ate agreement between the parties the System Quotation 1 12 Update means those releases of the Licensed Software that Company provides to customers to correct Errors fix bugs or create minor improvements incremental features or enhancements of existing features which Company designates by a change in the number to the right of the first or second decimal point Updates do not include those releases of the Licensed Software that provide substantial new features or additional functionality which Company designates by a change in the number to the left of the first decimal point 1 13 User means any individual that has an e mail address within the Enterprise 2 License and Restrictions 2 1 2 2 23 24 3 Support and Maintenance Services 3 1 32 License Grant Subject to the terms and conditions of this Agreement and the payment of the required fees set forth in the System Quotation Company grants to Customer a nontransferable nonexclusive royalty free revocable worldwide license without the right to sublicense to a install the Licensed Software on any computer located at any Site b use execute and display the Licensed Software in Executable Code form only and c copy the Licensed Software and Documentation solely as necessary to support Authorized Users in each of the foregoing solely in accordance with the Documentation and the Authorized Use Parameters Customer agrees that it will comply wi
28. ays for capillary and reagent storage and to carry out specific phases of the assay All trays can be opened and closed by selecting the Open Trays option from the Instrument menu in Compass software For more details please refer to the Opening Trays on page 64 ST Separation Tray Incubator Tray Upper Incubator Tray Lower Resource Tray Sample Tray Capillary Discard Tray Peggy User Guide page 28 Chapter 3 Peggy Resource Tray Size based Assays For size assays the resource tray houses the Running Buffer Wash Buffer Matrix Removal Buffer and up to two capillary boxes Wash Buffer Running Buffer Matrix Removal Buffer Reagents should be inserted into resource tray reagent holders as follows Wash Buffer left Running Buffer middle Matrix Removal Buffer right When using only one box of capillaries it should be placed in the pri mary box location closest to the reagents NOTE You an also refer to the labels on the resource tray for proper insertion of reagents Charge based Assays For charge assays the resource tray houses the Wash Buffer Anolyte Catholyte and up to two capillary boxes Peggy User Guide Instrument Overview page 29 Wash Buffer Anolyte Catholyte Capillary Boxes Reagents should be inserted into resource tray reagent holders as follows Wash Buffer left Anolyte mid dle Catholyte right When using only one box of capillaries it should be pla
29. ced in the primary box location closest to the reagents NOTE You an also refer to the labels on the resource tray for proper insertion of reagents Peggy User Guide page 30 Chapter 3 Peggy Sample Tray The sample tray houses the 384 well plate which contains samples and assay reagents cold block controls the temperature to 10 C over a portion of the sample plate while the remaining portion of the sample plate remains at ambient instrument temperature Cold Block Sample plates should be lidded and inserted into the sample tray so that the A1 well position is aligned with the upper left corner of the cold block A1 Position Sample Plate Peggy User Guide Instrument Overview page 31 NOTES Peggy requires that plate lids be used on sample plates Ifa lid is not detected a message will be displayed in the Start Run Wizard Compass software will reopen the sample tray to allow the user to insert a lid When inserting the sample plate ensure that the plate is firmly seated and level on the cold block Plates that are not level can interfere with the movement of the sample tray Incubation Trays Two incubator trays are used when Peggy is executing the incubation steps of an assay protocol Each tray can house up to 48 capillaries Peggy User Guide page 32 Chapter 3 Peggy Separation Tray The separation tray is used for the separation and detection steps of the assay protocol The separation
30. d if the water bottle empties or the waste bottle fills during the run The water bottle is not full Check the water bottle has enough water A The waste bottle is not empty Check the waste bottle has enough room If this occurs fill or empty each bottle as indicated using the procedures outlined in the next section Removing and Installing Bottles Bottles may be removed from the auxiliary module whenever Peggy is idle To remove or install the waste or water bottle please use the following procedure IMPORTANT Do not attempt to remove a bottle while Peggy is executing a run This may abort the run in prog ress 1 Remove the bottle by unscrewing it clockwise until it disengages from the manifold 2 Pull the bottle down slowly until the sensor at the bottom of the bottle is clear 3 Fill the water bottle up to the base of the neck or empty the waste bottle as needed Peggy User Guide page 40 Chapter 3 Peggy 4 To reinstall the bottle lift it up to the manifold making sure to enclose the sensor that sits at the bottom inside the bottle Screw the bottle counter clockwise until the arrow on the bottle s alignment label lines up with the arrow on the manifold s alignment label A WARNING BIOHAZARD Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available fr
31. dent abuse misuse negligence fire earthquake flood other force majeure event failure of electrical power the use of unauthorized products or unauthorized repairs or modifications b Company determines that an Error is caused during or as a result of delivery c a problem arises from or is based on Company s compliance with Customer s specifications or d Company determines that an Error is caused by malfunction of any hardware other than malfunction of the System or third party software used with the Licensed Software Disclaimer THE WARRANTIES ABOVE ARE EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER EXPRESS IMPLIED OR STATUTORY INCLUDING WITHOUT LIMITATION THE IMPLIED WARRANTIES OF MERCHANTABILITY FITNESS FOR A PARTICULAR PURPOSE TITLE AND NONINFRINGEMENT 5 Limitation of Liability NEITHER COMPANY NOR ITS SUPPLIERS SHALL BE RESPONSIBLE OR LIABLE WITH RESPECT TO ANY SUBJECT MATTER OF THIS AGREEMENT OR TERMS OR CONDITIONS RELATED THERETO UNDER ANY CONTRACT NEGLIGENCE STRICT LIABILITY OR OTHER THEORY A FOR LOSS OR INACCURACY OF DATA LOSS OF PROFITS OR COST OF PROCUREMENT OF SUBSTITUTE GOODS SERVICES OR TECHNOLOGY OR B FOR ANY INDIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES INCLUDING BUT NOT LIMITED TO LOSS OF REVENUES AND LOSS OF PROFITS COMPANY S AGGREGATE CUMULATIVE LIABILITY HEREUNDER SHALL NOT EXCEED THE GREATER OF FIVE HUNDRED DOLLARS 500 00 6 Term and Termi
32. eee e eee ne ees 3 SOLE s rri jaa EEA SA 4 User Attention Notifications 4 Chemical Hazard Sernak ecco tiees sanna 4 Chemical Waste Hazards 0 eens 5 Waste Production and Disposal 6 Material Safety Data Sheets 8 Instrument Safety Labels 0 c cece ees 8 Customer Service and Technical Support 10 LEGGINOHECS 222225023 Jahren tT caagiet 1 Peggy Disclaimer of Warranty 245 11 Compass Software and Authorization Server License Agreement oe isre oeo eee ees 11 Chapter 2 Lab Requirements 15 IAETOGUCUON sa Aar ena uit OEA Beas CAAA 16 Space REQUIFEMENTS 6 ccc cece cece cece eens 16 Electrical Requirements cece ee ee es 18 Environmental Requirements 0 cece eee 18 Software and Computer Requirements 19 Peggy User Guide pagei General Guidelines and Information 19 System Operation Notification of Intended Use 19 Lifting and Moving the System Use Proper Lifting Preca OS iced coe a abbot meres 19 Chapter 3 PEGGY im sn Boek SE set Ra a ke eat 21 instrument OVErvieW na ki N 22 External COMponents n 22 Internal COMPONENTS ser EE EE EERE 25 Auxiliary M A sara 38 Removing and Installing Bottles 39 Connection PANE ag 4 COMPIOR GE a A 42 Safety Guidelines ga teh Soe A 42 Doorintenock recer nenn yieee an a a 43 SVS
33. en Assay P Ready PI File Edit Instrument Window Help New Assay gt Open Assay gt MEK assay Protein Test Save Seve aes Peggy Charge Peggy Charge 1017 Import Protocol Erk Assay Import Template Export Protocol Export Template Print Exit xOnmoomD c Alist of the last five assays opened will display Select one of these assays or click Browse to open the Assay folder and select a different assay d Alternatively choose New Assay and select Peggy Charge to get the default Peggy assay condi tions e The Start button will display START BUTTON this indicates an assay is already loaded 4 a Go to the Assay screen and verify this is the assay you want to use If not select File in the main menu click Open Assay and select another assay 2 Click Start This will launch the Start Run Wizard Ready Stat NOTE If the manifold was not cleaned prior to starting the run a message indicating this will display If this occurs click Yes to cancel the run and perform the manifold cleaning Peggy User Guide page 56 3 Chapter 4 Operating Peggy Page 1 Check Water and Waste The fluid levels in the accessory module bottles will be checked by the software If the levels in both bottles will allow Peggy to complete the run the wizard screen will dis play Water Level OK and Waste Level OK messages Click Next to proceed Start Run a Chec
34. ession or control or return such copies to Company and d upon request by Company Customer shall certify in writing to Company that it has returned or destroyed such Licensed Software and Documentation The parties rights and obligations under Sections 1 Definitions 2 4 Ownership 4 3 Disclaimer 5 Limitation of Liability 6 Term and Termination and 7 General shall survive termination of this Agreement 7 General Peggy User Guide page 14 Chapter 1 General Information TA 7 2 73 74 LS 76 7 7 78 79 Assignment This Agreement and Customer s rights hereunder may not be assigned to any third party by Customer except with the prior written approval of Company Any attempted assignment of this Agreement or any rights or obligations hereunder will be null and void Governing Law This Agreement is made in governed by and shall be construed in accordance with the laws of the State of California without regard to any conflicts of law principles that would result in application of laws of any other jurisdiction The United Nations Convention on Contracts for the International Sale of Goods does not apply to this contract Any legal action or other legal proceeding relating to this contract or the enforcement of any provision of this contract must be brought in any state or federal court located in Santa Clara County California Customer and Company expressly and irrevocably consents and submits to the jurisdiction
35. f Test Assay Self Test Instrument Peggy Peggy SW1001 SW1001 Started Sat 4 53 PM Jun 9 2012 PDT NOTE ProteinSimple recommends performing the self test prior to starting a run To view the test log at completion of the test select Instrument click Properties and click View Test Log Peggy User Guide page 68 Chapter 4 Operating Peggy Viewing and Changing System Properties Select Instrument and click Properties to display system properties which include Name Location Type Serial number Instrument software version firmware Network name and address Date and time of the instrument clock r ia l Peggy SW1002 Properties Name Peggy SW1002 Location Type Peggy Network Name sw1002 local Serial Number SW1002 Network Address 10 1 2 158 Instrument Software 2 0 16919 Instrument Date and Time 2012 10 18 12 45 29 07 00 mia Gaia To change system name or location click in the name or location boxes and enter the new infor mation To sync the instrument clock with the computer click Set to PC time Viewing Error and Test Logs Select Instrument and click Properties to display system properties To view a log click either View Error Log or View Test Log Peggy s Status Modes The instrument status bar displays status buttons and progress bars depending on what Peggy is doing Ready New Run button Peggy is ready but an assay is not loaded Click New Run
36. gy User Guide page 76 Chapter 5 Maintenance and Troubleshooting NOTE Please contact Protein Simple Technical Support with any questions regarding the manifold clean ing procedure System Decontamination The following decontamination procedures should be performed after analysis of hazardous samples on Peggy Prior to Hazardous Sample Analysis The following procedure should be performed to prepare Peggy for hazardous sample analysis Items needed 10 bleach solution 1 Remove and empty the waste bottle 2 Add 10 bleach solution to the bottle until the liquid level is 1 cm from the bottom of the bottle 3 Reinstall the waste bottle Post Run Decontamination When running hazardous samples perform the following procedures monthly at minimum or as often as needed based on the type of samples being run This procedure should also be followed prior to system shipment Items needed 10 bleach solution 5 Contrad solution 0 22 micron filtered deionized water molecular biology grade or better 50 mL disposable syringe 1 mm syringe tip rubber gloves and paper towels 1 Perform the Wipe Down procedure see below 2 Perform the Internal Instrument Cleaning procedure see below 3 Perform the Waste Stream Cleaning procedure see below Wipe Down Procedure Items needed 10 bleach solution and towels NOTE Wear rubber gloves for the wipe down procedure 1 Completely saturate a towel with 10 bleach s
37. imary position first and if additional capillaries are needed use the secondary position When the primary box is depleted Peggy will automatically move to the secondary box Discard leftover Running Buffer Wash Buffer and Matrix Removal Buffer prior to refilling bottles for new run Peggy User Guide page 58 Br MH Un S Start Set up the instrument to start a new run Load the Wash buffer Anolyte and Catholyte 0664 Catholyte Wash Anolyte Load a primary cap box Add a secondary cap box if more capillaries required Primary Cap Box Secondary Cap Box lt Back Next gt tart Cancel Chapter 4 Operating Peggy NOTE You an also refer to the labels on the resource tray for proper insertion of reagents Primary Capillary Box Secondary Capillary Box Peggy User Guide Wash Buffer Anolyte Catholyte Starting a Run Charge based Assays page 59 6 Page 4 Load Sample Plate The sample tray will automatically open Place the lidded 384 well plate on the cooling block in the tray ensuring that the AT well position is aligned with the upper left corner of the cold block When this is complete click Next The sample tray will close A1 Position Peggy User Guide Start Run Load Sample Plate Remove the old sample plate and load the new plate 12345678 90NLHUHETBHMADDM A B c D E F G H 1 J K L M N o P vVOZLErXC IONMmDOU gt
38. is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Controlling Peggy The instrument menu allows users to control Peggy Window Help New Run Open Trays Manual Clean Cleanup Self Test Leveling Runs Properties Disconnect NOTE Instrument menu options are active only when a computer with Compass software is connected directly to Peggy Starting a New Run To start a new run select Instrument in the main menu and click New Run Then follow the steps described in Step 2 Start the Run on page 46 for size assays or Step 2 Start the Run on page 54 for charge assays Peggy User Guide page 64 Opening Trays Chapter 4 Operating Peggy To open any of the five trays select Instrument and click Open Trays The tray control window will appear Resource Tray Sample Tray amp Open Trays Main Trays k A Open a tray by clicking on its button The button will become highlighted indicating the tray is open NOTE Only one tray can be open at a time Open Resource Tray amp O
39. k water and waste Make sure there is enough water and room in the waste bottle LL Waste Water The run will be terminated if the water bottle empties or the waste bottle fills during the run Water level OK Waste level OK lt Back Next gt Start Cancel NOTE If the waste level is too high or the water level is too low to complete the run messages to indicate one or both will be presented in this screen If this occurs fill or empty each bottle as indicated using the procedures outlined in the Removing and Installing Bottles on page 39 When this is complete the error status will be automatically updated and allow the Start Run Wizard to proceed Peggy User Guide Starting a Run Charge based Assays page 57 4 Page 2 Replace Sponge A new sponge should be used each time a new experimental run is started Discard the old sponge and put a new sponge in the manifold wash station Start Run Replace Sponge Put a new sponge in the manifold wash station 5 Page 3 Start The resource tray will automatically open Fill the Wash Buffer Anolyte and Catholyte cups and insert the capillary box in the primary capillary box position Add a secondary capillary box if more capillaries are required Click Next when finished The resource tray will close NOTE Make sure enough capillaries are loaded to complete the run A capillary box should be inserted in the pr
40. l and health regulations Read and understand the Material Safety Data Sheets MSDSs provided by the manufacturers of the chemicals in the waste container before you store handle or dispose of chemical waste Minimize contact with chemical waste Wear appropriate personal protective equipment when han dling chemicals e g safety glasses gloves or clothing Use precaution when emptying the waste bottle Dispose of waste bottle contents in accordance with good laboratory practices and local state pro vincial or national environmental and health regulations Peggy User Guide page 6 Waste Production and Disposal It is the responsibility of the user to properly dispose of waste The aqueous bulk waste stream is approxi mately pH 8 For reference ProteinSimple disposes of waste under the non RCRA Hazardous Waste Liquid code 141 buffer containing sodium chloride surfactants A typical waste profile for one cycle 12 capillaries is as follows NOTE Volumes should be adjusted accordingly to account for running of additional cycles Waste Origin Size based Assays Volume Trough Reagents for 1 cycle Matrix Removal Buffer 2 0 mL Wash Buffer 2 0 mL Running Buffer 1 6 mL Water for trough and manifold cleaning 80 0 mL Total waste per cycle 85 6 mL Assay Plate Reagents Separation Matrix 0 240 mL Stacking Matrix 0 240 mL Biotinylated Ladder 0 005 mL Antibody Diluent
41. l regulation inability or delay in obtaining materials acts of God war earthquake fire or flood In the event of such force majeure the time for delivery or other performance will be extended for a period equal to the duration of the delay caused thereby provided that Company notifies Customer of the nature and duration of such force majeure event Entire Agreement Notice This Agreement constitutes the complete agreement between the parties and supersedes all prior or contemporaneous agreements or representations written or oral concerning the subject matter of this Agreement Except as otherwise expressly provided in this Agreement any modifications of this Agreement must be in writing and agreed to by both parties Company may provide any notice to Customer by e mail Customer may provide notice to Company by sending an e mail to info proteinsimple com or a letter by United States mail to ProteinSimple 3040 Oakmead Village Drive Santa Clara CA 95051 or to such other address as Company may specify in writing by posting the new address on the Company website 7 10 Relationship of the Parties The parties are acting hereunder as independent contractors and not as partners agents fiduciaries or joint venturers Neither party has the power or authority represent act for bind or otherwise create or assume any obligation on behalf of the other party Peggy User Guide page 15 Chapter 2 Lab Requirements Chapter Overvie
42. labo ratory use Part 1 General requirements US EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and labo ratory use Part 1 General requirements EU CAN CSA 22 2 No 61010 1 04 Safety requirements for electrical equipment for measurement con trol and laboratory use Part 1 General requirements CA EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC Require ments General requirements EU Safety Guidelines WARNING If the unit is not used as specified by the manufacturer the overall safety will be impaired WARNING If the unit is damaged and does not function properly stop the unit safely and contact ProteinSimple Technical Support immediately WARNING No user replaceable serviceable parts except for the ionizer fan and separation block CAUTION Avoid using Peggy in a manner not specified by ProteinSimple While the system has been designed to protect the user this protection may be impaired if the instrument is used improperly Peggy User Guide Safety Guidelines page 43 Door Interlock A door interlock is engaged when a run is started to prevent users from opening the doors during a run This eliminates any dangerous user interactions with the robot as well as exposure to high voltage and UV light A yellow indicator light indicates when the instrument is running and the interlock is engaged When the system status is Read
43. ll steps of the assay are performed in a single capillary for each sample and the system is capable of running up to 96 samples per experiment External Components System Door Right Status Lights System Door Left IWARNING There are no user replaceable serviceable parts except for the ionizer fan Peggy User Guide Instrument Overview page 23 System Doors Peggy s doors provide access to the inside of the instrument for the loading of samples reagents and capil laries To open the doors first confirm that the green System Status Ready Indicator is illuminated Using the handle located to the right of the System Status Indicators pull the right door open first Open the left door by pulling forward on the top and bottom latches To close the doors close the left door first and engage both latches then close the right door The right door secures with a magnet NOTE Peggy s doors must be closed before starting a run or cleaning protocol Door Interlock A door interlock is engaged when a run is started to prevent users from opening the doors during a run This eliminates any dangerous user interactions with the robot as well as exposure to high voltage and UV light A yellow indicator light indicates when the instrument is running and the interlock is engaged When system status is ready and the cover is open the robot s system tray Z stage motors and UV power supply are disabled IWARNING D
44. nation 6 1 6 2 6 3 Term of Agreement The Agreement is effective on the date Customer downloads the Licensed Software and shall remain in effect until terminated by either party as provided in this section Termination For Material Breach Either party may terminate this Agreement upon written notice if the other party materially breaches this Agreement and fails to cure such breach within thirty 30 calendar days following receipt of written notice from the other party specifying the breach in detail Notwithstanding the foregoing Company may immediately terminate this Agreement and all licenses granted hereunder if Customer breaches Section 2 License and Restrictions hereof or upon termination of the System Quotation The foregoing rights of termination are in addition to any other rights and remedies provided in this Agreement or by law Effect of Termination Upon termination of this Agreement or termination or expiration of any license granted hereunder all rights of Customer to use the Licensed Software and Documentation will cease and a all license rights granted under this Agreement will immediately terminate and Customer shall promptly stop all use of the Licensed Software and Documentation b all Services will terminate immediately c Customer shall promptly erase all copies of the Licensed Software from Customer s computers and destroy all copies of the Licensed Software and Documentation on tangible media in Customer s poss
45. nd 0 05 the instrument will need to be leveled Follow the instructions below or consult ProteinSimple Technical Support or your Customer Service Engineer to assist you in properly leveling the instrument Jam Nut Foot Nut Foot 1 Loosen the jam nut 11 16 on both front feet Peggy User Guide Maintenance page 75 2 Adjust the foot nut 1 2 on both front feet with a wrench until the level indicates 0 0 05 NOTE Turning the wrench clockwise will raise the front and make the sensor read more negative 3 Once level lock the position by holding the foot nut in place with the wrench and tightening the jam nut by hand Instrument Cleaning Instrument cleaning can be performed automatically See Cleaning on page 64 Manifold Flush Items needed 5 Contrad solution 0 22 micron filtered deionized water molecular biology grade or bet ter 50 mL disposable syringe 1 mm syringe tip and paper towels 1 Select Manual Clean from the Instrument menu in Compass software This will position the manifold for easy access and turn on the vacuum 2 Fill the 50 mL disposable syringe with 5 Contrad solution Place the 1 mm tip on the syringe 3 Flush each hole located on the bottom of the manifold Position the syringe tip into the first hole and inject 15 mL of the 5 Contrad solution Repeat for each manifold hole 4 Repeat step 3 with water 5 When all flush steps are complete click OK Peg
46. o not override the cover interlock The interlock protects users from exposure to UV light and a potential pinch hazard that could be caused by the moving system trays Peggy User Guide page 24 Chapter 3 Peggy Status Lights Three LEDs are located on Peggy s front panel to indicate system status Ready Green Indicates Peggy is powered on and ready for use Running Yellow Indicates a run or a cleaning protocol is in progress Error Red Indicates an error has been detected Error information will be presented in the Status window of the Run Summary Screen in Compass software Leveling Feet Four leveling feet are located at the base of the instrument The instrument must be level for proper opera tion During installation adjustments to obtain proper instrument level will be made by the ProteinSimple representative and this will be checked during preventive maintenance visits The level status of the instru ment is monitored internally and reported to the user through a Compass software interface Leveling Foot NOTE If Peggy must be moved from the original install location or requires leveling for any reason please see Leveling the Instrument on page 74 for leveling procedures or contact ProteinSimple Technical Sup port Peggy User Guide Instrument Overview page 25 Internal Components Head Assembly Robot X Stage Gripper Robot Z Stage Manifold Syringe Pump Door Latch Head
47. olution 2 Wipe down the exterior of the instrument Peggy User Guide Preparation for Storage or Shipment page 77 3 In Compass software select Instrument in the main menu and click Open Trays selecting the Separa tion Tray to extend the system tray Wipe down all accessible areas paying particular attention to a The upper and lower levels of the capillary box racks in the resource tray b The underside of the wash station in the resource tray c Close all trays Internal Instrument Cleaning Procedure 1 Empty and rinse the Running Wash and Matrix Removal Buffer cups 2 Fill each cup with 10 bleach solution and insert into the resource tray Waste Stream Cleaning Procedure 1 Select Manual Clean from the Instrument menu in Compass software This will position the manifold for easy access and turn on the vacuum 2 Fill the 50 mL disposable syringe with 10 bleach solution Place the 1 mm tip on the syringe 3 Flush each hole located on the bottom of the manifold Refer to the picture in Manifold Flush on page 75 for proper insertion of the syringe then position the syringe tip into the first hole and inject 15 mL of the 10 bleach solution Repeat for each manifold hole 4 Repeat step 3 with 5 Contrad solution 5 Repeat step 3 with water 6 When all flush steps are complete click OK NOTE Please contact Protein Simple Technical Support with any questions regarding the manifold clean ing procedure
48. om the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Read and understand the Material Safety Data Sheets MSDSs provided by the manufacturers of the chemicals in the waste vial before you store han dle or dispose of chemical waste Peggy User Guide Auxiliary Module page 41 Connection Panel The auxiliary module s connector panel is located on the lower left panel Auxiliary Module Vacuum Control VACUUM BLUE AIR BLACK A WASTE la D I WATER gt f en X_E a te RN en Water Waste Compressed Air Compressed Air Compressed air is provided to Peggy to power pneumatic actuations The com pressed air line is black Waste Waste produced by Peggy is emptied into the waste reservoir This line is under vacuum when waste aspirations are being performed Vacuum Vacuum is provided to Peggy for use in pick and place operations within the instrument Water This connection provides water to Peggy Peggy User Guide page 42 Chapter 3 Peggy Compliance Peggy complies with UL61010 1 2001 Safety requirements for electrical equipment for measurement control and
49. page 60 Chapter 4 Operating Peggy NOTES Peggy requires that plate lids be used on sample plates Ifa lid is not detected a message will be displayed in the Start Run Wizard Compass software will reopen the sample tray to allow the user to insert a lid When inserting the sample plate ensure that the plate is firmly seated and level on the cold block Plates that are not level can interfere with the movement of the sample tray 7 Page 5 Data File The data file name will automatically default to the assay name appended with the current date and time To change the file name begin typing in the text box To change the directory where the data file will be stored click Browse Start Run 2 Begin the Automated Run The following protocol will be run You can change the results location and prefix Assay Protein Test Cycles 8 Schedule Overlapping with hold Run name 2012 10 16 09 29 06 Protein Test Location C Users pfung Documents Compass Runs 1 Se EG Click Start to begin the run Instrument status will change to running and the stop button and progress bar will display Peggy User Guide Starting a Run Charge based Assays page 61 2012 05 30 15 52 06 DEXTOK 2mil Running SSS Wed 3 58 PM Thu 11 28 AM u The run will continue until complete 14 19 hours depending on the assay Step 3 Post Run Procedures 1 Empty the capillary discard tray 2 Remove the as
50. pen Trays Main Trays Other Trays gt jpper Incubator Lower Incubator if i To close a tray click the corresponding tray button again NOTE If the tray control window is closed when a tray is open the tray will close automatically Cleaning Peggy s cleaning can be performed manually by the user or automatically by Compass software Peggy User Guide Controlling Peggy page 65 Re Open Trays u Manual Clean e ESES Cleanup i Self Test Sample Prin Leveling Runs Properties Disconnect Manual Clean This option is used for general manual cleaning and cleaning the manifold head To do a manual cleaning select Instrument and click Manual Clean The manifold head will move to a safe position for easy access and the vacuum will turn on See Manifold Flush on page 75 for the manual manifold cleaning procedure NOTE Please contact Protein Simple Technical Support with any questions regarding the manifold clean ing procedure Cleanup This option is a fully automated cleaning step The manifold head is flushed the separation tray troughs are aspirated and washed and any capillaries left in the trays or gripper are picked up and discarded This option should be selected when the instrument has not been used for more than a week or if a run error occurs Cleaning takes about eight minutes to complete To start the protocol selec
51. proteinsimple Peggy User Guide Copyright 2013 ProteinSimple All rights reserved ProteinSimple 3040 Oakmead Village Drive Santa Clara CA 95051 Toll free 888 607 9692 Tel 408 510 5500 Fax 408 510 5599 email support proteinsimple com web www proteinsimple com Peggy User Guide P N 041 816 Revision 3 June 2013 For research use only Not for use in diagnostic procedures Patents and Trademarks Automatic Image Capture AIC and digital ProteomeChip technology are covered by U S Patent Nos 6 995 901 6 909 459 6 853 454 6 271 042 7 166 202 and other issued and pending patents in the U S and other countries ProteinSimple the ProteinSimple logo the Alpha Innotech logo Protein Forest the Protein Forest logo the AIC logo AlphaCal Alphalmager AlphaPart11View AlphaQuant AlphaSnap AlphaSpec AlphaUV AlphaView ChemiGlow Chromalight dPC digital ProteomeChip FluorChem MSRAT Multilmage NanoPro the intertwined helix design iWB Peggy ProteomeChip red Sally Simon Simple Western Spectra Plex Xpedition XplorBright and XplorUV are trademarks or registered trademarks of ProteinSimple Other marks appearing in these materials are marks of their respective owners Table of Contents Chapter 1 General Information 1 Welcome n e E 2 Simple Western Assays ON Peggy 0 0 c cece ceca ee 2 Size Based Assays c cence eee ees 2 Charge Based ASSAYS 0 2 cece
52. roperly Customer Service and Technical Support Telephone 408 510 5500 888 607 9692 toll free Fax 408 510 5599 E mail support proteinsimple com Web www proteinsimple com Address ProteinSimple 3040 Oakmead Village Drive Santa Clara CA 95051 USA Peggy User Guide Legal Notices page 11 Legal Notices NOTE Read the Legal Notices carefully before using Peggy Peggy Disclaimer of Warranty EXCEPT AS EXPRESSLY PROVIDED IN ANY ProteinSimple SOFTWARE LICENSE AGREEMENT OR QUOTATION THE PRODUCTS SOLD AND SER VICES PROVIDED BY ProteinSimple ARE PROVIDED ON AN AS IS AND AS AVAILABLE BASIS WITHOUT WARRANTY OF ANY KIND ProteinSimple AND ITS SUPPLIERS DO NOT WARRANT THE SECURITY PRIVACY OR ACCURACY OF ANY DATA PROVIDED VIA THE PRODUCTS OR SERVICES AND YOU AGREE THAT THE USE OF ANY SUCH DATA BY YOU IS AT YOUR SOLE RISK TO THE MAXIMUM EXTENT ALLOWED UNDER APPLICABLE LAW ProteinSimple AND ITS SUPPLIERS DISCLAIM ANY AND ALL WARRANTIES WHETHER EXPRESS IMPLIED OR STATUTORY INCLUDING WITHOUT LIMITATION ANY WARRANTY OF MERCHANTABILITY TITLE NON INFRINGEMENT OR FITNESS FOR A PARTICULAR PURPOSE Compass Software and Authorization Server License Agreement IMPORTANT PLEASE READ CAREFULLY THE TERMS OF THIS COMPASS SOFTWARE AND AUTHORIZATION SERVER LICENSE AGREEMENT AGREEMENT BY CLICKING ON THE AGREE BUTTON 1 YOU ACKNOWLEDGE THAT YOU HAVE READ UNDERSTAND AND AGREE TO BE BOUND
53. rs Physical Specifications Description Specification Dimensions 0 84M H x 0 94M W x 0 61M D 2 76 H x 3 08 W x 2 00 D Weight 82 kg 180 Ibs Power Requirements 100 240 VAC 50 60 Hz self adjusting Power Consumption 650 W Operating Humidity Range 20 60 relative non condensing Operating Temperature Range 18 24 C 64 75 F Table 3 1 Physical Specifications For indoor use only Use up to altitudes of 1524 meters 5000 feet Peggy User Guide page 45 Chapter 4 Operating Peggy Chapter Overview Power Up Starting a Run Size based Assays Starting a Run Charge based Assays Stopping a Run Waste and Water Bottles Controlling Peggy Peggy Status Modes Shutdown Peggy User Guide page 46 Chapter 4 Operating Peggy Power Up 1 A WN Turn on the computer connected directly to Peggy Turn on Peggy s main power switch Wait for Peggy to initialize Open Compass software Starting a Run Size based Assays Step 1 Get Ready 1 w Open Compass software Prepare instrument empty waste refill water and add a new manifold sponge Create or open desired assay file Prepare assay plate following the procedure described in the product insert IMPORTANT To prevent well evaporation and ensure best results keep a lid on the assay plate until ready to use While plate is spinning add Wash Running and Matrix Removal Buffers to resource tray cups Place cap
54. s have already been cleaned click OK to continue If the troughs in the separation tray are empty click on OK and proceed with Cleanup on page 65 If Running Buffer is present in the separation tray click on Cancel and manually remove the buffer Evapora tion of the Running Buffer will result in a highly viscous residue which the automatic cleaning feature cannot remove To remove the Running Buffer 1 Select Instrument and click Open Trays Peggy User Guide Controlling Peggy page 67 Open Trays Main Trays Other Trays Separation pt Resources Samples i 7 i i y f X 2 Click Separation to open the separation tray 3 Add 800 uL of deionized water to the troughs in the separation tray and soak for 20 minutes 4 Remove the water by either aspirating with a pipette or with the vacuum wand located on the inside of Peggy s left door 5 Repeat the steps above until the Running Buffer or residues are completely removed 6 Tocomplete the cleaning process select Instrument and click Cleanup Self Test Peggy can perform a series of self tests to check for proper instrument performance To start the test select Instrument and click Self Test The test takes approximately two minutes Compass eis File Edit Instrument Window Help Self Test E Assay EE Analysis Sat 4 53 PM Sat 4 55 PM E Separation lt N Plot SI Run Sel
55. say Multi Western_Hela HUT78 Cycles 8 Schedule Overlapping with hold Run name 2012 05 31 09 29 06 Multi Western Hela HUT78 Location C Users pfung Documents Compass Runs t Click Start to begin the run Instrument status will change to running and the stop button and progress bar will display Peggy User Guide Starting a Run Size based Assays page 53 2012 05 30 15 52 06 DEXTOK 2mil Running SSS Wed 3 58 PM Thu 11 28 AM u The run will continue until complete 14 19 hours depending on the assay Step 3 Post Run Procedures 1 Empty the capillary discard tray 2 Remove the assay plate 3 Dispose of the assay plate and capillaries Disposal will depend on the samples that have been assayed If sample origins are unknown ProteinSimple recommends that used capillaries and plates be disposed of in biohazard waste WARNING SHARPS HAZARD The capillaries may present a potential sharps hazard Dispose of used capillaries in biomedical waste sharps containers IWARNING BIOHAZARD A Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the
56. say plate 3 Dispose of the assay plate and capillaries Disposal will depend on the samples that have been assayed If sample origins are unknown ProteinSimple recommends that used capillaries and plates be disposed of in biohazard waste WARNING SHARPS HAZARD The capillaries may present a potential sharps hazard Dispose of used capillaries in biomedical waste sharps containers IWARNING BIOHAZARD A Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbl5 Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Read and understand the Material Safety Data Sheets MSDSs provided by the manufacturers of the chemicals in the waste vial before you store han dle or dispose of chemical waste Peggy User Guide page 62 Chapter 4 Operating Peggy Stopping a Run 1 To stop a run click Stop When the run stops instrument status will go to Not Ready and a Cleanup but ton displays a Not Ready NOTE Ifa run is stopped prior to completion Peggy must
57. stitute effective notice of the changes described in the notice If Customer does not agree with the changes to this Agreement Customer must notify Company prior to the effective date of the changes that Customer wishes to terminate its license to the Licensed Software Continued use of the Licensed Software following notice of such changes shall indicate Customer s acknowledgement of such changes and agreement to be bound by the terms and conditions of such changes Severability In the event any provision of this Agreement is held to be invalid or unenforceable the remaining provisions of this Agreement will remain in full force Waiver The waiver by either party of any default or breach of this Agreement shall not constitute a waiver of any other or subsequent default or breach Export Customer agrees not to export reexport or transfer directly or indirectly any U S technical data acquired from Company or any products utilizing such data in violation of the United States export laws or regulations Force Majeure Company shall not be liable directly or indirectly for any delay or failure in performance of any obligation under this Agreement including any delivery obligation where such delay or failure arises or results from a cause beyond Company s reasonable control or beyond the reasonable control of Company s suppliers or contractors including but not limited to strike boycott or other labor disputes embargo governmenta
58. t Instrument and click Cleanup A window will appear with instructions F Cleanup Make sure the separation tray troughs are clean before clicking OK To clean the troughs click Cancel and use the Open Trays menu to access the separation tray troughs Cleaning the troughs may require soaking them with distilled water If the troughs have already been cleaned click OK to continue Peggy User Guide page 66 Chapter 4 Operating Peggy Peggy s status will change to cleaning and the stop button and the cleaning progress bar display The Assay screen provides cleaning status details a When cleaning is complete instrument status will change to Ready Cleaning ZA Assay C Run Summary Analysis Cleaning NN Sat 4 45 PM Sat 4 53 PM File Edit Instrument Window Help us Ready Additional cleaning steps are required if an error occurs that stops the run When this happens the red Error status light on Peggy s front panel will come on see Status Lights on page 24 Cleaning After a Run Error Click on the Reset button displayed in Compass software The following instructions will appear r hl Cleanup Make sure the separation tray troughs are clean before clicking OK To clean the troughs click Cancel and use the Open Trays menu to access the separation tray troughs Cleaning the troughs may require soaking them with distilled water If the trough
59. th the Authorized Use Parameters License Restrictions Customer acknowledges that the Licensed Software and its structure and organization constitute valuable rade secrets of Company Accordingly the license granted in this Agreement is subject to the following restrictions Customer and its Authorized Users a may not reverse engineer disassemble decompile or otherwise attempt to derive the source code of Licensed Software b may not modify adapt alter translate or create derivative works from the Licensed Software c may not merge the Licensed Software with other software d may not use the Licensed Software in any service bureau or time sharing arrangement icense sell rent lease transfer assign distribute host outsource disclose or otherwise commercially exploit or make the Licensed Software or Documentation available to any third party e shall only make that number of exact copies of the Licensed Software and Documentation as delivered by Company that are necessary to support Customer s use of the Licensed Software in accordance with his Agreement f shall include any titles trademarks and copyright and restricted rights notices that are included on or in the Licensed Software as delivered by Company on and in any copies of the Licensed Software that it makes and g shall ensure that Customer s use of the Licensed Software does not exceed the scope of the license that Customer has purchased pursuant to this Agreement
60. thernet RJ 45 connector is used to connect Peggy to a computer or local network NOTE Serial numbers are used to identify individual instruments on the network Peggy User Guide Instrument Overview page 37 Auxiliary Module Connections Module Control This connection allows communication between Peggy and the auxiliary module Compressed Air Compressed air from the auxiliary module is used to power pneumatic actuations in Peggy The compressed air line is black Waste Waste produced by Peggy is emptied into the waste reservoir in the auxiliary module This line is under vacuum when waste aspirations are being performed Vacuum Vacuum is provided to Peggy by the auxiliary module and is used in pick and place opera tions within the instrument Water This connection provides water to Peggy from the auxiliary module Peggy Instrument Rear Panel System Label Asystem label is located on the rear panel of Peggy It includes the following information the ProteinSimple location system model power requirements serial number and certification markings The serial number of the instrument can also be found on the lower left side panel above the power entry Peggy User Guide page 38 Chapter 3 Peggy Auxiliary Module The auxiliary module houses the water and waste bottles used by Peggy For identification purposes the waste bottle is labeled in yellow and the water bottle is labeled in blue The volume in each reser
61. to open an assay Peggy User Guide Shutdown page 69 Ready Start button Peggy is ready and an assay is loaded Click Start to begin a run Not Ready Clean button Peggy is not ready and must perform system cleaning Click Clean to start the cleaning protocol Not Ready Reset button Peggy is not ready and must reinitialize Click Reset to start the initializa tion protocol Running Stop button Peggy is running an assay The run name time the run started and when it will complete display in the run progress bar Click Stop to stop the run Cleaning button not active Peggy is running a cleaning protocol The time the cleaning protocol started and when it will complete display in the run progress bar Error Reset button An error has occurred Go to the Status window in the Run Summary screen to view details When the source of the error is corrected click Reset Shutdown 1 Close Compass software and shut down the system computer 2 The instrument can remain on unless it will not be used for an extended period Peggy User Guide page 70 Chapter 4 Operating Peggy Peggy User Guide Chapter 5 Maintenance and Troubleshooting Chapter Overview Software Updates Maintenance Preparation for Storage or Shipment Spare Parts Troubleshooting Peggy User Guide page 71 page 72 Chapter 5 Maintenance and Troubleshooting Software Updates To check for software updates go to Compass software select
62. up of viscous material in the head NOTE A fresh manifold sponge should be replaced after each run or when setting up a new assay plate and experiment Peggy User Guide Instrument Overview page 35 User Vacuum A user vacuum line is located on the inside left panel of the instrument This vacuum is used for instrument cleaning procedures and can also be used to clean liquid spills when needed The vacuum turns on when ever Peggy s doors and a system tray are open at the same time To use the vacuum turn the valve to the on position Turn the valve to the off position when not in use Peggy User Guide page 36 Chapter 3 Peggy Peggy Instrument Left Panel Located on the lower left side of Peggy is her serial number power entry power switch and connector panel Serial Circuit Network Compressed Number Breaker Connection Air Vacuum Auxiliary Module Switch Control Power Power Input Waste Water System Serial Number This label contains the serial number for Peggy System Power The main system power components consist of the power switch and power input WARNING Only use the power supply cord provided with Peggy If the cord is damaged please contact Pro teinSimple Technical Support WARNING No user replaceable parts WARNING SHOCK HAZARD Disconnect the power cord from the instrument power input to disconnect power to the instru ment Network Connection A 10 100 BASE T E
63. voir is 1 0 L Deionized water is used by Peggy to clean the troughs in the separation tray and the fluidics in the head assembly The waste bottle collects waste generated by Peggy during run execution For more information on Peggy waste production please refer to the Waste Production and Disposal on page 6 4 Manifold mn Waste Reservoir at gt Water Reservoir Alignment Labels Alignment Labels Waste Reservoir Water Reservoir Drip Tray There are two liquid level sensors in each bottle The sensor located in the bottom of the bottle indicates when the bottle is empty The sensor located in the top of the bottle indicates that sufficient volume is avail able to complete an eight cycle assay with the instrument NOTE ProteinSimple recommends that users check bottle levels prior to starting a run to ensure the assay will run to completion Fill the water bottle up to the base of the bottle s neck before starting a run If the waste bottle becomes full or the water bottle becomes empty when a run is executing the run will be aborted Peggy User Guide Auxiliary Module page 39 The Start Run Wizard in Compass software will also perform a level check When a full or empty status is detected the software will present this error condition in Page 1 of the Start Run Wizard Start Run LR Check water and waste Make sure there is enough water and room in the waste bottle Waste Water A A The run will be terminate
64. w Introduction Space Requirements Electrical Requirements Environmental Requirements Software and Computer Requirements General Guidelines and Information Peggy User Guide page 16 Chapter 2 Lab Requirements Introduction This chapter will help you prepare the lab for Peggy Please complete the space electrical vacuum and envi ronmental requirements prior to scheduling your installation NOTE Please wait for an authorized ProteinSimple representative to unpack and install Peggy Do not attempt to do this as improper handling of the instrument and computer can result in personal injury or damage to the system Space Requirements Please provide adequate space to accommodate the system and computer with sufficient clearance for heat ventilation and service accessibility Peggy is a bench top unit that requires a sturdy lab bench or table that can support 200 Ibs 91 kg IMPORTANT Peggy must be on a stable surface and level at all times for proper operation Ensure the lab bench or table does not shift or wobble under heavy weight Additionally anti vibration tables may not be used as they can alter the instrument level during operation Peggy User Guide Space Requirements System dimensions are as follows Dimension Meters Feet Peggy Width 0 94 3 08 Width with doors open 1 27 4 17 Depth 0 61 2 00 Height 0 84 2 76 Auxiliary Module Width 0 33 1 08
65. waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations Read and understand the Material Safety Data Sheets MSDSs provided by the manufacturers of the chemicals in the waste vial before you store han dle or dispose of chemical waste Peggy User Guide page 54 Chapter 4 Operating Peggy Starting a Run Charge based Assays Step 1 Get Ready 1 ek WN Open Compass software Prepare instrument empty waste refill water and add a new manifold sponge Create or open desired assay file Prepare assay plate following the procedure described in the product insert IMPORTANT To prevent well evaporation and ensure best results keep a lid on the assay plate until ready to use While plate is spinning add Wash Buffer Anolyte and Catholyte to resource tray cups Place capillary box in the designated resource tray position IMPORTANT Capillaries are light sensitive Keep the cover on the box until you are ready to transfer the capillary box to the resource tray Place assay plate into the sample tray of the instrument and press Start Step 2 Start the Run 1 You can start a run in one of two ways depending on what button is displayed in the status bar NEW RUN BUTTON click New Run Peggy User Guide Starting a Run Charge based Assays page 55 b Select File in the main menu and click Op
66. y and the doors are open the robot s XZ stage motors high voltage power supply and UV power supply are disabled IWARNING Do not override the cover interlock The interlock protects users from exposure to UV light and a potential pinch hazard that could be caused by the moving system tray System Trays CAUTION Do not attempt to open or close any of the system trays manually Instead use the Open Trays command in the Instrument menu in Compass software Auxiliary Module Reservoirs IMPORTANT Do not attempt to remove water or waste reservoirs while Peggy is executing a run This may abort the run in progress Peggy User Guide page 44 Chapter 3 Peggy Waste Disposal Hazard IWARNING BIOHAZARD A Samples and waste bottle contents should be handled by procedures recommended in the CDC NIH manual Biosafety in Microbiological and Biomedical Laboratories BMBL The manual is available from the U S Government Printing Office or online at http www cdc gov biosafety publications bmbls Depending on the samples used waste bottle contents may constitute a biohazard Use precaution when emptying the waste bottle Dispose of waste bottle contents in accor dance with good laboratory practices and local state provincial or national environmen tal and health regulations IWARNING SHARPS HAZARD Capillaries may present a potential sharps hazard Dispose of used capillaries in biomedical waste sharps containe

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