XFA6000 Auto Hematology Analyzer USER'S MANUAL
Contents
1. 0 30 EE EE 39 Etienne 30 4 11 HOKE VS ageet ea Geelen ad aia arang kg aka aan Chapter 5 Setup 32 5 1 Printing Format 30 5 2 Version a aa ba a NN aaa ja a NN NNN NNN a D a NNN NNN NNN aa ana a aaa a Waa ana 33 5 3 Time ET 34 EAO ei Ge ese le AA 35 GEES E TT 37 eee EE 37 fe Ale EE 39 Chapter 6 Sample 6 6 lt 8 88 8 88 8 88 8 8 8 88 8 8 88 88 8 8 8 8 888 8 88 8 888 41 6 1 Sample REVIOW 41 6 1 1 Histogram Review 9 42 6 2 Sample Inquiry 44 6 2 1 ID Inc E 44 Chapter F ll HE 44 GETRAUT A5 7 2 System E 45 7 2 1 Replace Diluent a 46 7 2 2 Replace Hemolysin sree 46 SE AG 7 2 4 LTT Program a a a 29 A7 7 2 5 5 2 POO INE 48 TOBAGA a a a E2. ai E Gy Figure 1 8 Figure 1 9 1 Power board 3 A D board 2 Main control board 4 Amplification board Control panel 20 keys and Start key avail your operation Key function START Start counting operation in the interface of Counting Auto demarcation and Q C counting Add diluent in status of Add diluent MENU From windows operation to menu operation or from menu operation to windows operation PRINT Default setting is auto print Press lt key to switch to manual print SILENCE Turn off alarm and clear alarm information O 9 Input numbers lt In windows move the cursor gt Set the upper limit of PLT ID Inout sample ID ENTER To confirm CLEAN Clean the liquid pass Display Caption Status Indication Windows ON Line Help Figure 1 10 Status Indication Area Display the current system status Operation status Sampling mode Windows Area Display results and operations Remind Area Remind you the operation procedures Caption Area Current windows caption Clock Area Display system time Operation Windows and Menu operation Windows operation Edit browse and operation in windows area press MENU to operation Menu operation Press 1 lt to select press ENTER to what you select and get into windo
3. Note Do not leave the diluted sample too long time so as not to ensure the accuracy of counting Keep dust from prepared diluent 3 5 Blood Sample Counting Whole blood counting Operate in Whole blood counting interface 26 Automatic Hematology Analyzer Thole Blood Idle Common V2 4 HO 00000000 ID 90000 WEC il Hii di 3 0 107A bhas 0 1 rand AB 10 JA LT H 00 Mig 00 0 50 100 150 200 250 fl Grani 00 HOB 0 oi 2 REC 1 00 4 RI 00 L L MEY fl MOH 0 0 50 100 150 200 250 f MCHC 0 yL ROW y 0 0 3 ROW sd 0 0 fl d PLT 1 kt L Maa 1 Pll 5 D L L 0 10 15 20 25 A DAN 0 0 T GC HEHU Measure Counting ENTER Demarcate 11 11 11 Figure 3 8 1 Setup print format Get into Setup interface to select print format Chinese parameter name with histogram Chinese parameter name without histogram English parameter name with histogram English parameter name without histogram If you want to connect external printer first ensure the right connection of the printer Turn on the power supply of printer and the instrument will be identified with the outer printer in work and printed after finishing blood sample analysis to the outer printer 2 Counting operation Put vein anti coagulation blood under the sampling pin press Start to count Remove the sampling tube after the sampling pin lifting and the instrument beeping At this time the i
4. Mid X WBC Mid 100 Gran X WBC Gran 100 HGB HGB ConstantxLog 10 Blank Photocurrent Sample Photocurrent RBC Structure front electrode back electrode onfice Figure 1 4 RBC RBC nx10 L MCV The instrument counts the pulse of each RBC when it passes sampler classifies it according to volume and then calibrates it to count the MCV HCT MCH MCHC RBCXM ere 10 HGB L MCH pg II RBC L HGB g L MCHC g L EE x 499 HCT RDW RDW is calculated directly from histogram PLT PLT nx10 L MPV MPV is calculated directly from histogram PDW PDW is calculated directly from histogram Block monitoring Animal blood contains protein and other grains which may block in pipe line and orifice and affect the results To solve this problem the instrument will have a high voltage burn after each sampling and avoid by daily and weekly maintenance On the other hand the instrument has a function of block monitoring once the counting time exceeds setting range the instrument will alarm to remind the customers 1 3 Performance Principle for counting and volume calculation Resistance method Parameters Parameter Abbreviation Unit Basic parameters WBC 10 L RBC 10 L HGB g L PLT 10 L From histogram Ly MO GR MCV fL RDW sd fL RDW cv MPV fL PDW From calculation Ly 10 L MO 10 L GR 10 L HCT L L MCH pg MCHC g L
5. PERLONG XFA6000 Auto Hematology Analyzer USER S MANUAL CONTENTS Chapter 1 Introduction PPE CIEE eg CEC CECE aa aa aa aa a anaa aa eee ee er aran aa aa 8 9 aa aa aa aa eee a 8 8 ree ere a a aaa a aan aaa a re oe 1 EE 1 ECKE EE 3 EE 8 1 4 Structure and Operation eaaa eee anaa eaaa 10 Chapter 2 Installation 2 2 58 2 5222 225 22 52 22 55 2 2 5 22 25 2 7 55 22 5 2 5 2 5 2222522 2282553 255 255 14 SE E E 14 2 2 Installation Requirement 14 SCHT 1 11 15 Chapter 3 Sample Analysis SE 17 fe EE 17 SE EE 18 SEI EK 19 3 4 Blood Sample Colection ec nes daa a da dada dada daa ba d NG ANAN St 3 5 Blood ET EE St BEEN 3 Chapter 4 Maintenance eege 29 A CLT ECE aaa na Cr rr err ren terry aa naga aaa aa mre rn nn errr rrr nr tn 29 Aa e E 30 Ee TE TT 30 4 4 Drain Counting Chamber kana sana eba naka sak anaha sakanan nanak ekan aka nahen basaha ENING KNA 30 4 5 Dran Pipeline i asana A Nagan pa 31 d Valve Pump Detection sss eerste ea eaae 31 5952711991 212101 E 31 4 8 Orifice Voltage Detection ss eesese
6. In the interface of MENU move the cursor to MAINTENANCE Drain Chamber press ENTER to start draining the counting chamber 4 5 Drain Pipeline In the interface of MENU move the cursor to MAINTENANCE Drain Pipeline press ENTER to start draining the pipelines 4 6 Valve Pump Detection In the interface of MENU move the cursor to MAINTENANCE Valve Pump Detection press ENTER to get the interface as showed in figure 4 3 and start detecting the working status of every valves and pumps V Pump Checking Vi Pump Check Press Meru to Exit 11 11 11 Figure 4 3 Press ENTER when the cursor is in a certain position the status of related valve pump will change from Off to On or from On to Off You can hear the noise of the change Please detect the malfunction of valve oump according to these 4 7 Motor Detection 34 In the interface of menu move the cursor to MAINTENANCE Motor Detection press ENTER to get the interface as showed in Figure 4 4 Ml Moto M2 Moto M3 Moto Figure 4 4 In the menu of motor detection choose 1 Lift Motor 2 Position Motor 3 Injector Motor by choosing 1 3 After choosing a motor press N to choose the moving position of the motor and then press ENTER to confirm 4 8 Orifice Voltage Detection In the interface of MENU move the cursor to MAINTENANCE Orifice Voltage Detectio
7. 51V power supply Malfunction of 15V power supply malfunction WBC A D malfunction RBC A D malfunction PLT A D malfunction HGB malfunction Low vacuum no paper Reagents PL 6A Diluent PL 6A Hemolysin HB Washing Solution High concentration Enzyme HB E Z Washing Solution Power supply Voltage AC 220V 10 50Hz Frequency 150 VA Fuse AC 250 V 2A Operating condition Temperature 10 C 35 C Relative humidity 85 Atmospheric pressure 70 0 106 0kpa Transport and storage condition Temperature 10 C 40 C Humidity lt 93 Atmospheric pressure 50 0 106 0kpa Dimension 40cmX39cmX46cm L A W XH Weight 25Kg 1 4 Structure and Operation Main frame ID 11 E a Ch BIG Tig 51 lt H Kl Jahal kr pr ht ta Figure 1 5 Front Panel 1 LCD screen 2 Panel keyboard 3 Recorder 4 Sampling pin 5 Start Key MEASURE key Figure1 6 Rear panel 1 USB joint 2 Printer connector 3 Keyboard joint 7 Waste liquid connector 8 Power Switch 9 Ground pole 4 RS 232 output 10 AD board writing soft connector 5 Diluent connector 11 Main control board writing soft connector 6 Hemolysin connector Figure 1 7 1 Sampling pin 5 Injector 2 Swab 6 Motor 3 Counting chamber 7 Vacuum chamber 4 Valve 8 Pump NA H 3 4 9 D 6 7 8
8. for modification Press lt and move the cursor among the items Press Enter and save the amended content Press MENU to exit All the 19 parameters in the reference value range have reference value recommended by the factory and adjust default value according to ID BLY KR RDVey RD ed 1 4 Select MENUJExit ENTER SAVE 11 15 11 Figure 5 15 General Reference Value 42 11 0 15 0 17 0 0 106 0 282 Figure 5 18 Children Reference Value 43 Select MENU Exit ENTER SAVE ll 11 11 Figure 5 19 Newborn Reference Value Chapter 6 Search Totally 20000 samples and histograms can be stored in this machine The system will automatically save the data after testing each sample The operator can review all the parameters and histograms print the saved data by inner printer or an outer printer delete data inquire samples the inquiry results can be showed in a list or the machine can separately single sample with histogram Sample searching includes Sample Review and Sample Inquiry 6 1Sample Review Press MENU and choose SEARCH as in Figure 6 1 the system will show Sample Review and Sample Inquiry There are 2 types of sample review one is review with histogram and the other is list review without histogram Mode Maint ain setup E Help Power OFF Figure 6 1 In the MENU interfa
9. not shut down within 24 hours please use HB enzyme concentration cleaning liquid to dip in 4 Equipment re starts If the equipment turns off as to power cut or other reasons please wait 30 seconds after Start up
10. quantitatively in laboratory The testing items are 1 WBC 2 Li 3 MO 4 GR 5 LY 6 MO 7 GR 8 RBC 9 HGB 10 HCT 11 MCV 12 MCH 13 MCHC 14 RDW CV 15 RDW SD 16 PLT 17 MPV 18 PCT 19 PDW It also provides the histogram parameters of WBC PLT and RBC Classification Class Type B Continuous working instrument Maintenance the instrument can not work normally please read Chapter 9 for your reference problem can not be solved please feel free to contact us Reagents calibrator and standard These 3 items compose a system and make sure that all these are proved by the manufactures We recommend our own hematology reagent kits All our reagents are calibrated and proved by our instrument Do not use other reagents or calibrators or it may affect the results Every reagent should be checked before use Container damage may cause the quality of reagents If the packing is damaged please check that the products is of leakage of damped Do not use the reagent if the above situation occurs Make sure to realize the other entire Notes about the reagents The reagent should be stored and used at room temperature Notice To use the reagents correctly and safely please do read the 3 label and data table Diluent Diluent dilutes the blood sample and at the same time provides the blood cell an environment that is similar to plasma Hemolysin It can destroy RBC rapidly and shrink i
11. repaired or when there 15 a warp found in quality control please calibrate it 7 5 Parameter Adjustment Choose Parameter Adjustment in SERVICE to enter parameter adjustment See Figure 17 and 7 18 Mode Maintain status Setup Assistant Service Info Query Adjust Help Debug Figure 7 17 2 4 88 215 Adsl Whe Alz HL CY 5 D Adj EP edir A We gtt E Zgrtt Adjel 1 Vor idit TN Adjust Fb gitt Presa WERT frit dea Figure 7 18 Move the cursor to the parameter required to be adjusted press TI to change the parameter value or status Press ENTER to confirm the changed value press MENU to exit 7 6 Waveform Display The list contents are used to provide apparatus maintenance and must input password of superuser to use it 56 Mode Maintain status Setup Assistant gt Seryice Info Query Adjust Help Debug Power OFF Display Figure 7 19 Chapter 8 Help Besides online help during operation there is also a help interface in the system to help the users to solve the problems during operation and judge malfunctions quickly Mode Maintain setup Service Huer Help Power OFF Figure 8 1 Move the cursor to HELP in MENU then press ENTER to enter the help interface Interface Thanks for using 8000 Full automatic Hematology Analyzer software Version 2 4 Us
12. select object press 1 3 to select discriminator and lt to move discriminators Note that if the WBC result is less than 0 5 or exceeds the range the WBC histogram is ineffective Four discriminators of the WBC histogram are adjustable and two discriminators of the RBC histogram are adjustable and two discriminators of the PLT histogram are adjustable For example move the second discriminator of the following WBC histogram to 10fL the adjustability is only for taking examples and actually the histogram isn t required to adjust 1 Press ENTER into adjustment interface 29 D 0 100 150 200 250 D 50 100 150 200 250 4 D 5 10 15 320 25 Figure 3 9 2 Press 2 to select the second discriminator 0 50 100 150 200 250 Figure 3 10 3 Press gt to move to 100fL 8 o 0 100 150 200 250 Figure 3 11 4 Press MENU to quit 50 100 150 200 250 8 Figure 3 12 Clean Clean the sampling pin WBC counting chamber RBC counting chamber and pipelines Press Clean to operate Clean swab Advise to clean the swab if the instrument works for 30 days or test 2000 samples Advise to replace the swab if the instrument works for 300 days or test 30000 samples Pack Pack the instrument before long distance transportation 1 Drain the liquid pass 2 Clean the pipelines with distilled water 3 Drain the pipelines 4 Fix the sampling assembly 3 7 Shutdown Perform the Shut
13. 0 1 Lo reng On 1 LT 00 Wha 3 00 U 50 100 150 200 250 Grant op PGB gi gt REC LON soli HT op L C MEY fL MCH 0 0 Pe D 50 100 150 200 250 A MCHC gL ROW v 00 ROW 24 0 0 Fl PLT 107A 1 Ad d PCT 0 00 L L E 0 15 20 25 Wi D D CH FEN Measure Counting ENTER Demarcate 11 11 11 Figure 3 4 Malfunction information erase malfunction information will be erased automatically only when the malfunction disappears Status Indication Area From left to right on upper right side of the screen it is Running Mode Running Status Reference Value and Version as displayed in Figure 3 4 Running Mode Whole blood Pre dilute and Paracentesis Press Mode to select Running Status Get ready and Test Test In Ready status press Start to counting Reference Value Adult Male Female Children Newborn Press Reference Value to select Sample mode whole blood and per dilute 23 Whole blood The instrument is working under whole blood mode Press Start the sampling pin will suck in 13ul whole blood sample Pre dilute The instrument is working under pre dilute mode Press Start the sampling pin will suck in 6 1 55 diluted sample System Time current time Number Sample Number 0 99999 Upper left side of the counting area Display current patient s No Press ID and then press ENTER to amend current pa
14. E 48 7 2 7 Packing 48 Eelere Dee eet 49 7 4 Parameter Calibrating tettette eee 50 99 82 1 8 1 1 9 1 50 7 4 2 User System Calibrating Eeee KEN trenean tr eener reenen ernennt 54 7 4 3 User Hematology Control sss aee eaaa eaaa anaa eaaa anae anna aee aana nean aee e anae treener teneat 5 7 5 Parameter Adjustmeni etetett ren nannte Ennan E Ene 53 7 6 Waveform Display 53 Chapter 8 Help 66 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 54 Appendix Packing List EE 57 CHAPTER 1 INTRODUCTION 1 1 Summarize This user s manual includes operation and maintenance in order to keep this instrument in good performance please do follow this manual and usage This XFA 6000 Auto Hematology Analyzer is an in vitro diagnostic instrument used to clinic and to analyze the blood cell
15. Principle for HGB measurement Hemoglobin absorbance medium wave length 540nm Sampling Reagent consumption for each measurement Whole blood vein blood 13yL Pre diluted sample twig blood 20uL Calculation volume 400uL with Hemolysin and diluent every WBC measurement 10 Hemolysin volume Diluent ratios 300uL second time diluent every RBC and PLT measurement 0 5mL whole blood 0 5mL twig blood Whole blood twig blood WBC HGB 1 270 1 263 RBC PLT 1 45000 1 45000 Orifice dimension WBC orifice RBC orifice Measuring time Performance Displayed Range Para WBC 10 L RBC 10 L Hb g L MCV fL PLT 10 L Linearity range Para WBC 10 L RBC 10 L HGB g L MCV fL PLT 10 L Repeatability Para 100um diameter 60um L 70um diameter G um CL less than 1 minute Range 0 0 99 9 0 0 9 99 0 300 0 250 0 999 Linearity range 0 0 99 9 0 0 9 99 0 300 40 150 10 999 Repeatability CV WBC 7 0 15 0 10 L RBC 3 5 6 0 10 HGB 110 180g L MCV 80 0 110 0fL PLT 150 500 10 Display Colorful LCD 640x480 lt 2 lt 1 5 lt 1 5 lt 0 5 lt 4 11 Input and output RS232 output Printer connector Keyboard connector USB connector Read in connector for main board software Read in connector for A D board software In build Printer optional EPSON LQ 300K Alarm Malfunctions of injection motor Motor M1 Motor M2
16. R to get the interface EE Clock 2413 1 bey Mee L Teston leak adrera Pirca MEW to Ze Operation Press f to choose Press lt to adjust Figure 5 6 Reference p SETUP Time Setup and press If you dont adjust any parameter after entering this interface the system will continue timing you only amend year month or day and the system will continue timing with hour minute and second 5 4 Gain Print Set Mode Version Maint ain Sequerce Setup Time Service Gain Query Syat en Help Password Fower OFF Reference 38 Figure 5 7 Adjust the gain of WBC RBC PLT and HGB In the interface of SETUP Password only when you set the password as you can adjust the parameters in this interface In the interface of MENU move the cursor to SETUP Gain press ENTER to get the interface as showed in Figure 5 8 Plus Adjust Press MENU to Exit 11 11 11 Figure 5 8 WBC Gain Adjustment The purpose of WBC gain adjustment is to adjust the figure of WBC histogram U amp 100 180 209 250 1 Figure 5 9 LI i E Vd E GE ees E E D 80 100 190 200 20 tL Figure 5 9 1 If the histograms of most samples are as showed in Figure 5 9 the gain of WBC may be too small please enlarge the gain If the histograms of most samples are as showed in Figure 5 9 1 the gain of WBC may
17. WBC lt 0 2 RBC lt 0 02 HGB lt 1 1 10 IE Hiem E MAN mm Jm BA Am H EDTA D I St lu hr Sum br A EDTA AHE 1 5 2 2me ml ffi Hr m br A DURE Km SA FRA LM E ERREA PTL M KRR HR 0 88 18 BE HKEE PAM 11m ARR ERJ E KEAN E DV DI 20 1 ANG RASA A 1 1m BRAE GEA EIER 8 1 Uu Fr EE RRR IEE TN FABRA op A A Zil AUWA EADE 5 0 FERED NGA RE 147977 TANEM Tri S r KAN TAK a se HR 08 Hp RSET ANU KET SA 1 gha rtez AUKA E RENEI SS Im HSG MAVE EARMA HIE SSH 0 5V zZ AJE 10 35 C METI Gut 1 38381 6 3 tata eet 908 VET HELA WRAT dr A E ONES ATL CHRD bann 8 PR T URERVE te SAH ARS ERR 8 BE 2 SAAR RIGA wu Se AH EH PERRIER FE ASTI va BEA HB 8 8 58 8 HB PRS PEAS Se JNF 100 IY tH 3 AH HB 86 8 ur HB RAF PERSIE o WR 24 181 WAE 1 KAY UR HB RAR AOE 6 IARTA 7 RARE ALS HSER ISR Das ta 30 P aAA 61 Annex VI XFA6000 brief operations manual and notes 1 Preparation before startup Check whether reagents are sufficient or not 2 Chec
18. alibrating or Whole Blood User Coefficient Calibrating Before calibrating please choose work mode in MODE then after calibrating the coefficient will be automatically stored in Whole Blood or Pre dilute mode Mode Selection Password Input enter User Calibrating according to the above route and press ENTER to enter the interface as showed in Figure 7 10 Based the test error measure calibrating coefficient of input parameters Press MENU return the interface of counting Farameter Adjust WRC Adjust Li Adjust MO Adjust RAC Adjust WY Adjust FDW Adjust PLT Adjust DV Adjust POW Adjust HB Adjust Press MENU to Exit ii 11 11 Figure 7 15 7 4 3 User Hematology Control Calibrating There is the function of User Hematology Control Auto Calibrating and the new calibrating coefficient will be calculated after testing calibrating sample The operating procedures are as follows 1 Enter SETUP Password input as password 2 Set the mode to Whole Blood or Pre dilute 3 Choose User Hematology Control Calibrating according to the above steps and then press ENTER and the LCD will show as Figure 7 16 54 8000 Auto Adjust System 1 03 05 OF O8 11 13 15 17 193 21 23 25 27 29 31 02 04 08 08 10 12 14 18 18 20 22 24 28 28 30 WAC GE peur iso 88511 0 111111 delist Figure 7 16 4 Input the Item No Lot No Pe
19. ally and please read carefully and join into the manual to refer Maintenance Service lf the machine cannot work normally please refer to the related statement in Chapter 8 of the manual 1 2 Test principle Cell counting The blood sample adopted by this instrument should be diluted in certain ration firstly and then measure WBC RBC PLT and HGB Diluted sample in counting chamber of WBC and RBC pass through the counting orifice under the effect of negative pressure the quantity pass through the orifice is the sample volume to analyze This instrument uses impedance method to measure distribution and quantity Two electrodes are placed on each side of the orifice which makes an electric field When the cell passed through the orifice electrode resistance increases proportionately with the cell volume Refer to Figure 1 1 fi electrode electrode Wood sample counting chamber orifice Figure 1 1 pulse time Figure 1 2 Control of measuring sample volume Volume calculation This instrument calculate volume by time and in the condition of certain vacuum when diluted sample passes through RBC70um and WBC100ul orifice it proportionately with the time Sample dilution In blood the cells joint together causes the difficulty to count correctly and sizing the cell volume so we need to dilute the blood to get the blood cell to pass the orifice individually And at the same time it also provides electric
20. be too big please reduce the gain RBC Gain Adjustment the MCV value of quality control is 5 larger or smaller than the preset value please adjust the RBC gain to adjust MCV teat value The adjust scale is as follows 39 MCV QC reference value is 90 0fL MCV QC test value is 98 0fL MCV QC reference value 60 mm MCV QC test value 98 0 918 Set the adjustment rate of RBC to a value most near 91 8 HGB Gain Adjustment To change the background voltage by adjusting the HGB gain Adjust the HGB background to 4 4 4 5V 5 5 System Setup In the interface of MENU move the cursor to SETUP System Setup press ENTER to get the interface as showed in Figure 5 10 Print Set Mode Version Maintain Sequence setup SeEVLICE Gain Query Syet em Help Password Power OFF Reference Figure 5 10 The LCD willshow System Setup as figure 5 11 1 1 2 IS E P izing 3 11 11 Jzrp Ot SR 11111 Sestra tat artiy Figure 5 11 Operation Press ENTER to choose the content modified 40 Press t 4 to change the item value Press MENU to exit 5 6 Password Print Set Mode Version Maint ain Seguente setup Time Service 7 3 11 Query Syet em Help Password Figure 5 12 In the interface of menu move the cursor to SETUP Password press ENTER to get the inte
21. boratory operating rules Warning Wastes must be disposed of according to the local national regulations Pipelines and power cables check Check the diluent hemolysin and whether waste liquid pipelines bend and connection is reliable and the plug of power supply is safe to insert the socket of power or not Recorder and printer check Check whether the paper used in recorder and printer is enough or not and the installation is at the right place and the printer power supply insert socket of power and the cable of printer connected with the mainframe is ready or not Note When turning on the power of outer printer the machine will be identified with the data record to outer printer The inner thermal sensitive is ineffective If needed to print on the inner thermal sensitive printer please turn off the power supply of outer printer 3 2 Startup lf there is an outer printer press printer switch to get it ready Mainframe Startup 1 Turn on the switch 2 The instrument will make an initialization automatically and the screen will display System detection this process may last about 80 seconds 3 Initialization interface displays as in Figure 3 1 The system will check hardware automatically and clean the pipelines and make the background determination 21 Welcome for using AUTO HEMATOLOGY ANALYZER Caries CE akung Figure 3 1 The instrument will get into counting interface after self check Aut
22. ce move the cursor to SEARCH Sample Review press gt the system will show the 2 choice items of Sample Review as showed in Figure 6 2 44 Single Browse Start 000 Total 0 111 Press MENU to Exit 11 11 11 Figure6 2 6 1 1 Review with histogram Press ENTER to edit the sample and press lt gt to review the sample one by one gradually and press MENU to exit as showed in Figure 6 3 Single Browse Ho 00000000 ID 00000 da UU h re Wa 30 100 150 2 25 Start 00000 DOLI Press MENU to Exit 11 11 11 Figure 6 3 When reviewing to a certain record press ID key and it will delete the record and the system will warn as seen in Figure 6 4 If confirmed to delete the cursor moves to Y to finish the deletion 45 Single Browse HO 00000000 ID LT bhag Grant La JE Gran a IO 150 200 25 Start 00000 Total DO111 Dress MENU to Exit 11 11 11 Figure 6 4 Press PRINT to print the data you reviewed Press 7 while reviewing certain ID record the case history message will be out as seen figure 6 5 and the cursor is at the position of Name shown light red At this time you can edit sampling message for the ID number press TAB to move the cursor When required to modify certain message you can move the cursor to the column and modify by the keyboard The cursor moves
23. down procedure to shut down the analyzer daily Press MENU and lt to select Shutdown press ENTER to get into shutdown interface a dialogue box pops up as Figure 3 13 Service Query Help Power OFF Figure 3 13 Please Put Washing then Press Measure Key Please Input E_Z Washing then Press MEASURE Key 11 11 11 Figure 3 14 Put the HB E Z Washing Solution in the sampling pin and press MEASURE key and pop up a dialogue box as Figure 3 15 25 1 Power OFF Program Please Put E_Z Washing then Press Measure Key Please Input E Washing then Fress MEASURE Key 11 11 11 Figure 3 15 After shutdown procedure pop up a dialogue box as Figure 3 16 and the screen will indicate Please Turn Off Power OFF Program Please Turn Off Please Turn Off aka a Figure 3 16 Dispose of waste liquid Bio pollution Samples reagents hematology controls standards and waste liquid may have danger of contagion So please wear protection clothes and glove and please obey the laboratory operating rules Warning Wastes must be disposed of according to the local national regulations 32 Chapter 4 Maintenance This machine is a precise machine only correct operation and termly maintenance can guarantee long time and reliable performance Pollution caused by overflowed liquid and leak not only influence the test result but also influence the performance of the
24. e diluent away from dirt 8 After absorbing the diluent a drop liquid hangs on the sampling pin and adhibit the drop to sample cup with the wall of sample cup and mix up 7 2 4 LTT Program Choose LTT Program and press Enter key and pop up the figure 7 7 awaiting customers to put the liquid bottle of probe head to down the sampling pin and press Test and the apparatus is soaked into 1 6m probe head liquid and clean for counting chamber and twenty minutes later finished automatically The customers can press random key to intermit after 5 minutes and the liquid path is cleaned ahead of schedule and return the interface of counting LTT Program Put into sampling pin press Test to 5688 Press Test to Continue 11 11 11 Figure 7 7 7 2 5 E Z Program 50 Choose E Z Program and press Enter and the system pops up as figure 7 8 awaiting customers put E Z liquid to down the sampling pin and press Test and the apparatus is soaked into 1 6ml E Z liquid and clean for counting chamber and twenty minutes later finished automatically The customers can press random key to intermit after 5 minutes and the liquid path is cleaned ahead of schedule and return the interface of counting Put into sampling pin press Test to soak Press Test to Continue 11 11 11 Figure 7 8 7 2 6 Aging Aging is a process of apparatus manufacture and must input password to work normally Press Aging P
25. environment for counting and finishes the measurement of cell numbers and volume size In whole blood mode mix 13ul whole blood and 3 5m diluent to form a 1 270 mixture Divide the 1 270 mixed samples to two parts Mix 15 6ul mixed sample and 3 9ml diluent to form a 1 45000 sample which is used to counter RBC and PLIT Mix the rest sample Om Hemolysin and 1ml diluent to form 1 385 samples which is used to counter WBC In Pre dilute mode mix 20ul twig blood and 1 1ml diluent to form 1 55sample Add 0 7ml 1 55 sample and 1 1ml diluent into WBC counting chamber to form 1 263 sample Divide the 1 263 samples to 2 Mix 15 2 ul 1 263 sample and 3 9ml diluent to form 1 45000 sample which used to counter RBC and PLT Mix the rest 1 263 sample and 0 5m hemolysin to form 1 378 samples to counter WBC Before diluent add some Hemolysin into WBC diluted sample to dissolve WBC Cell volume Volume distribution after effect of Hemolysin and diluent as bellows WBC 30 400fL RBC 25 250fL PLT 2 30 For the difference of cell volume the instrument can counter the RBC and PLT individually In Figure 1 2 a for PLT pulse b and d for RBC pulse compared with PLT pulse c is so tiny that is considered as a circuit noise or cell scraps In threshold collecting process 8 15 countered as PLT and is deleted Doas WBC Structure Hemolysin Input Hemolysin Input Front Electrode HGB Light input Orifice HGR Light Back Electr
26. er a Interface Operation Panel Keys Mode Off Maintenance Set Service search Title Status Indication e Menu On line Help User Interface Press MENU to back Press TJ 1 to choose 11 11 11 Figure 8 2 57 Press MENU to enter 1111 To make the interface scroll Panel Key START Start counting spit diluent while adding diluent 0 9 Inout number Cleaning Clean pipelines reused as key 2 SILENCE Turn off the alarming noise reused as key 1 ID Enter the inquiry interface input sample No to inquire MENU Enter and exit from MENU PRINT Print ENTER Confirm the selected operation 1111 1 Move the cursor in window area and menu area Power Off Please make the following steps before turn off the power 1 Press MENU to start menu operation 2 Move the cursor to Power Off with N J 3 Press ENTER to start to execute the process of power off 4 Press ENTER to enter the Power Off interface the system will clue to add Washing solution 5 Put the Washing Solution under the sampling pin press START the pin will suck 1 1ml and then execute power off 6 After finishing power off the LCD will show Please Turn Off 7 Turn off the power switch 8 Dispose of the waste liquid Setup 1 Press MENU to start menu operation 2 Move the cursor to SETUP with J 3 Press
27. ery Help Power OFF Figure 3 5 Press MENU in Mode interface move cursor to Pre dilute and press ENTER to Pre dilute setup interface In this interface the background color changes obviously Press MENU to get into Counting press ENTER to counting interface Press 0 Diluent a dialogue box pops up as in Figure 3 6 25 Automatic Hematology Analyzer Thole Blood Idle Common 2 4 HO 00000000 ID 00000 yy 3 Op 47 oe 00 bhaa 00 e Grant 1 0 10 LT 0 00 1 255 J 00 Grant 00 HGE U 61 REC 2 RI 1 101 L L hit 0 MH 00 Pe 1 ID 150 MCHC V gL ROW v 0 0 5 PD 84 0 0 fl d PLT i 10 e HME 0 fl AC 0 0 LAL 0 5 15 POV 0 D T 16 WEN Measure Counting EHTER Demarcate Figure 3 6 Present a clean sample cup to the sampling cup is tilted towards the pin as Figure 3 7 shows Aspirate 1 1ml diluent each time Figure 3 7 U 50 100 150 Z200 250 200 250 D 11 11 11 pin and make sure the to avoid spills and bubbles Every time it can add the diluent of several cups continuously After that press MENU to exit 3 Configuration a Collect blood sample with 20ul sampling tube b Only use lint free tissue to wipe external wall of the tube c Add the sample into sample cup containing 1 1ml diluent d Mix to well distribution e Test after 2 minutes
28. he cursor to MODE by pressing fT 3 Press to start choosing 4 Move the cursor to the mode you need by pressing f 5 The items include Whole Blood Pre dilute Paracentesis 6 Press ENTER to start the selected mode Maintenance 1 Press MENU to start menu operation 2 Move the cursor to MAINTENANCE by pressing f J 3 Press to start maintaining 4 Move the cursor to the item needs to be maintained by pressing 7 J 5 The items include Deblock Cleaning Drain Chamber Drain Pipeline Aging Valve Pump Detection Motor and Orifice Voltage 6 Press ENTER to start Maintaining Service 1 Press MENU to start menu operation 2 Move the cursor to SERVICE by pressing J 3 Press to start service 59 4 Move the cursor to the service item you need by pressing 5 The items include System Status Packing Malfunction Information Parameter Calibrating and Parameter Adjustment 6 Press ENTER to start service Appendix Packing List HB Washing Solution High concentration 50ml bottle Bottle Bottle Bottle Bottle Enzyme HB E Z Washing Solution 20ml bottle Silicon Tube 60 MEX XFA6000 Iren Ja TE CG d 1 2 4 FP AL BY BI HE NATAH ae ALEEM WTA ACHAT BERR FHL 2 81168 1 801898 21 1 Ce Mar ATA Ra al 2 E FoR BE MAS ANAN IR A BEOK
29. information parameter calibrating parameter adjustment and system assistant Please press MENU and then press to choose SERVICE Press it will show as Figure 47 7 1 System Status Mode Maintain Setup Ase ist ant SeEVLCe Info Query Adjust Help Debug Fower OFF Display Figure 7 1 Service MENU Choose System Status in SERVICE the machine will enter system status showed in Figure 7 2 System Status Measure Mode Whole Blood Ref Rang Baby Print Mode 4 Program Space 1 501 Remain Space Sample S5Space 5 621 Remain Space 2211 HGE Voltage 5 00 Y Press Voltage 5 00 127 Voltage D 00 12V voltage 5 00 system Status Display Press MENU to exit 7 2 System Assistant Mode Maint ain setup Service Query Help Power OFF Choose System Assistant Figure 7 2 SYSTEM STATUS Status Hemolyein Assistant Info TT Wash Adjust CH EzZ Vash Debug Meine Display Pack Figure7 3 in SERVICE as showed in Figure 7 3 Press t to choose related task press ENTER to make system carry out the task Finally press MENU to quit 48 7 2 1 Replace Diluent Choose Replace Diluent and then press ENTER the system will show as Figure 7 4 and start to replace diluent after finishing replacement the system will automatically return the interface a
30. itive head the paper is out of the mouth and close the recorder Press the green light button and green light goes out Press the red light button and walk out of a length of paper The paper is printed normally and the fixture of record paper is over Press the green light button it is on and recorder is under the working condition Printer there is a printer equipped with the system please connect the printer cable to the printer joint on the machine Turn on the power of printer and operate Print and the result will be shown on the outer printer automatically As the detailed installation steps and operation method of the printer please refer to the user s manual of printer Chapter3 Sample analysis This chapter contains Operation preparation Startup Counting interface Blood sample collection Blood sample counting Shutdown 3 1 Operation preparation Before turning on the instrument operators should do the following process to ensure to be ready for the system and helpful to work normally for the machine Diluent and Hemolysin check Reagents consumption Type Diluent Hemolysin Washing Solution nm 1 1 mm 20 Waste liquid barrel check Prepare the container by operators themselves Empty the container Bio pollution Samples reagents hematology controls standards and waste liquid may have danger of contagion So please wear protection clothes and glove and please obey the la
31. k whether the reagents are cloudy or not deteriorated and make the 4 Empty the waste bottles 2 Start up Press the power switch to 1 location 2 Auto checking will run and it will come to test interface after auto checking has been completed 3 Background check Press the start button the machine counts the background The results of this request at the end WBC lt 0 2 RBC lt 0 02 HGB lt 1 PLI lt 10 4 Measurement of whole blood Press Menu key select Mode key and set the model to whole blood Taking EDTA2K as anticoagulant to distill anticoagulant specimens EDTA2K consumption 1 5 2 2mg ml blood Make anticoagulant mixing so that the sampling needle inserting anticoagulant Press the start key to measure samples 5 Measurement of peripheral blood Press Menu button to select the Mode item the mode is set to pre dilution Press 0 dilution key firstly then press the start button taking 1 1ml diluents under the sample needle according to Menu key back Adopts 20ul the end blood specimen from the capillarity sucker to pour rapidly into the 1 1ml diluents cup makes the dilution blood sample Note the acupuncture must be deep and have to use paper towels that can not hair to scrape the first drop of blood diluted blood samples should be shaken well with the thumb and index finger and hold the upper cup and with a wrist rotation about a minute or so pay attention not to severe concussio
32. machine Please wipe the machine immediately don t just air dry it If the leak is inside the machine operator or maintainer should repair it at once In this chapter you can learn the maintenance of the pipelines and the detection method of the other parts Maintenance includes 10 items Deblock Cleaning Drain Chamber Perfusion Chamber Drain Pipeline Valve Pump Detection Motor Detection Orifice Voltage Detection Burn and Motor Reset 4 1 Maintenance Deblock Clean D Chamber D Chamber Maint ain D Pipeline Y Pump Query Orifice Service MotorCheck Help Burn Power OFF Mot or E Figure 4 1 In the interface of MENU move the cursor to MAINTENANCE press ENTER to start maintaining The following 10 operations are maintenance of the pipelines to guarantee the reliable running of the machine Deblock Cleaning Drain the counting chamber Perfusion the counting chamber Drain the pipelines Valve oump detection Motor detection Orifice voltage detection Burn Motor reset 4 2 Deblock In the interface of MENU move the cursor to MAINTENANCE Deblock press ENTER to start deblock the pipelines and the orifice 4 3 Cleaning In the interface of MENU move the cursor to MAINTENANCE Cleaning press ENTER to start cleaning the pipelines with the HB probe head as seen in Figure 4 2 33 Cleaning Program Cleaning Please Waiting Figure 4 2 4 4 Drain Chamber
33. n press ENTER to start detecting and display the test value as showed in Figure 4 5 Orifice Voltage Display Orifice Voltage Figure 4 5 4 9 Burn the Orifice In the interface of MENU move the cursor to MAINTENANCE Burn press ENTER to start burning and cleaning orifice with high voltage 4 10 Motor Reset In the interface of MENU move the cursor to MAINTENANCE Motor Reset press ENTER to start resetting This operation is always done after operation on motor At 35 that time the motor is not in the normal counting position After motor reset the sampling pin will be in the counting and sucking position Chapter 5 Setup Many parameters in the system can be reset to meet needs of different users There are 7 options Print Format Version Time Adjustment Gain System Setup Password and Reference Value Move cursor to SETUP in MENU and then press ENTER to get the interface as showed in Figure 5 1 5 1 Print Format Print Set Mode Version Maint ain Se QUerc setup Service Gain Query Sraten Help Password Power OFF Reference j Figure 5 1 Move the cursor to SETUP Print Format press ENTER to get the interface as showed in Figure 5 2 Print Settings Sg uPrinter Parameter Lutter Printer Englise Ze utter Picture Cleaning Please Waiting Figure 5 2 Print Select Inner Printer O
34. n otherwise there is probably not allowed to count placed 5 minutes shake again Diluted blood samples will be disposed of at the sample needle press the start key count 6 shutdown Everyday the implementation of shutdown procedures must be executed through HB thick enzyme cleaning liquid after testing About one minute later the screen prompts to turn off the host then turn off the power supply Notes 1 environmental requirements Environment dustproof Power cord should have a good grounding and voltage not more than 0 5V Interior temperature of 10 35 C air conditioning should be allocated 2 Troubleshooting In case of plugging the holes holes voltage increase please click on the small keyboard Cleaning key to clear up if still plugging holes press the menu of safeguarding to execute the hole burning stones row blocking hole gem recoil and then count the end of time If a serious hole plugging execute many times and can not be resolved please contact after sales service department of our company 3 routine maintenance Per two months use hemolytic with distilled water to clean bottles and cap components attention to prevent pollution Sample size greater than 100 please use the probe HB every day to do implementation of a solution HB probe immersion cleaning fluid sample size of less than 100 please HB probe to implement a cleaning fluid HB probe immersion cleaning fluid in three days you do
35. nstrument is analyzing the sample during counting the indication shows the progress and it will finish about 1 minute later The instrument beeps again and sampling pin back to original position results will show on screen ID will plus one automatically 3 Pre dilute counting Put the diluted sample under sampling pin press Start to count When the pin lifts remove the blood sample and begin to test About 1 minute later the result will show on screen and ID plus one automatically Notice In sampling keep a distance between sampling pin and cup bottom or it will affect accuracy of test 27 After analysis result will show on screen and if Auto print is ON the result will be printed out automatically In counting if malfunction occurs alarm will show on Malfunction Information Area the temperature is higher than 35 C or lower than 10 C then the results may be not accurate Display results After analyzing the 19 parameters and 3 histograms WBC RBC and PLT will show on screen The linearity range is as bellows Parameter alarm the analysis result is followed by an H or L it means the analysis result exceeds the upper or lower limit of the reference range If you see as opposed to the result it means the result is either unreliable or out of the range If the WBC result is less than 0 5 x 10 L the instrument will not perform the differential analysis and all the related parame
36. ode Figure 1 3 WBC WBC nx10 L WBC Classification and calculation According to the size of WBC volume there is manifold configuration The volume size of various configuration isn t unchangeable Due to the difference of diluents hemolytic agents and hemolytic time the volume size of various parts will be changed Under the stated process control the chemical reaction can display three classifications of WBC lymphocyte middle cells granulocytes Lymphocyte is the smallest white cell Through the hemolytic agents The volume sizes of them are mainly distributed at the range of 30fL 85fL The middle cell between lymphocyte and granulocytes mainly has acidophilia basophilla monocyte After mixed with hemolytic agents their volume sizes mainly are distributed at the range of 85fL 125fL Granulocyte is the biggest white cell Under the effect with the hemolytic agents the epicyte is damaged and set out plasma and rudimental cytoplasm granule is compressed between nucleolus and damaged epicyte Generally speaking the granulocyte volume through the effect of hemolytic agents is distributed in the range of upper 125fL With directly measured on the distribution of pulse height through the orifice there is a histogram distributed by the volume of WBC According to the followed formula it can get the percentage of classifications PL w X100 PL PM PG PM Mid PL PM PG x100 P Gran IN X100 PL PM PG Lymph XWBC
37. om the accessory bag Take out the diluent barrel there should be enough diluent in the barrel Put one end of the pipe into the diluent barrel please refer to figure 2 1 and then tighten the barrelhead clockwise Figure 2 1 Diluent Connection Hemolysin connection Take out the hemolysin hose assembly from the accessory bag Connect one end of the pipe with the hemolysin bottle and the other end connecis the liquid path joint of the hemolysin brown on the back of the machine Tighten the bottle cover clockwise Figure 2 2 Hemolysin connection Waste liquid pipe connection Take the waste liquid pipe with red connector from the accessory bag Connect the red connector to the waste joint on the back of the machine And then tighten it clockwise The waste liquid barrel should be placed at the same height of the machine or lower than it Insert the other end of the waste liquid pipe into the waste liquid barrel Disposal of the Waste Liquid Bio pollution Samples reagents hematology controls standards and waste liquid may have danger of contagion So please wear protection clothes and glove and please obey the laboratory operating rules Notice Wastes must be disposed of according to the local national regulations Installation of recorder paper Press upper right button of recorder and open the recorder Put in the coil paper right towards formfeed mouth and printed side is up and towards thermal sens
38. omatic Hematology Analyzer Thole Blood Idle Common V2 4 HO 00000000 ID 90000 WEC Oo ov TK Li S HU d rY IN 0 0 ei rang 1 LT 0 OO Rea bhais I wll 150 SUE Zi D 165 Op Grant D op PGB gi 5 REC 0 00 HI 00 L L Mi VU 0 i MCH Pe U 50 100 150 200 240 A MH d gL ROW v 6 0 5 e DW sd 0 0 fl l PLT 0 du e Maa fi PCT Ab J L L E 1 1 5 E A POWY 0 OO A E GC HEHU Measure Counting EHTER Demarcate 11 11 11 Figure 3 2 As in Figure 3 2 show the result of the background determination The instrument will alarm if there is any problem in auto check Malfunctions display on the upper right side of the screen 3 3 Counting interface 1 instrument will get into counting interface automatically after startup When required to do maintenance the instrument will back counting interface after finishing operation Press MENU in counting interface to enter Menu operation interface Select your operation Maint ain setup Service Query Help Power OFF Figure 3 3 Malfunction Information Area in upper right area of counting interface displays malfunctions during operation refer to Figure 3 4 Automatic Hematology Analyzer Thole Blood Idle Common Wa 4 HO 00000000 00000 ll WEC 00 10 mee 3 AU 1 A bhas
39. r parameter calibrating Mode Maint ain status Setup Assistant je Service Info Query Adjust Help Debug Power OFF Display Figure 7 14 There are two ways that are factory calibrating and user calibrating provided by this apparatus and must input password in advance and can carry out the operation 7 4 1 Preparation of calibrating Use our calibrating samples and control solution to calibrate the machine the calibrating will be completed automatically by the machine There are 2 modes whole blood and pre dilute After calibrating the machine will automatically provide two calibrating coefficient Please check the machine before calibrating make sure that the machine is in normal status If there is any abnormality please stop calibrating Please check if the reagent and accessories are enough Please check the background to make sure that the background value can meet the requirements Repeat test the middle blood sample in the counting interface make sure the machine is in the precise range 53 Parameter Repeatability CV WBC 7 0 15 0x10 L lt 2 RBC 3 5 6 0 10 L lt 1 5 Hb 110 180g L lt 1 5 MCV 80 0 110 0fL lt 0 5 PLT 100 500 10 lt 4 Please record the following factors of every calibrating Date Lot No standard value background test value of WBC RBC PLT HGB and MCV 7 4 2 User Parameter Calibrating You can choose either Pre dilute User Coefficient C
40. rface as showed in Figure 5 12 and Figure 5 13 Password Config Password 44x Please Input Password Press Meru to Exit 11 11 11 Figure 5 13 Where it is required to input the password must return and input the correct password and carry out your operation 5 7 Reference Value Labs always set reference value based on normal person as figure 5 15 figure 5 19 H the test value is not in the normal range we always look this as clinical abnormity Operator can reset the upper and lower limit of the 19 parameters If the test value is out of the range there willbe an H or L after the test value on the screen or the print paper this is to show the test value exceeds the limit H the test value is higher than the upper limit 4 L the test value is lower that the lower limit Reference value can be modified but you must input correct password first Please write down the original data before modification Print Set Mode Version Maint ain sequence Setup Time Service Fair Male Query Sraten Female Child Help Password Power OFF Reference Newborn Figure 5 14 Move the cursor to Setup Reference the reference value you want The chosen will be regarded as the new reference value Press MENU to exit Press ID to modify reference value and the corresponding reference value table is listed on the table Press to choose the parameter waiting
41. riod of Validity and Reference Value of the hematology control 5 Test the hematology control after 3 tests the CV and calibrating coefficient will be automatically showed in the chart 6 The machine will ask if you accept the result after every test you may select either ACCEPT or QUIT 7 You can do 5 tests at most 8 Press ENTER after testing you can decide whether the result is effective Press ENTER to confirm and save the new coefficient Press QUIT to give up the new coefficient Before select Factory Calibrating or User Calibrating you should first enter related password in SETUP Factory Calibrating is done by the factory to guarantee the quality of the machine After leaving the factory during transportation or use there may be excursion so please hematology control the machine termly Please calibrate the machine if system warp is found to guarantee the veracity of the result Notice The machine must be calibrated otherwise the test result cannot be used as effective data The calibrating uses specimen and reagents in order to guarantee the quality please use specimen and reagents recommended by our company And please store the samples correctly don t use samples which are not in period of validity Before calibrating please make the temperature of sample back to the environmental temperature and mix the sample 55 Frequency of calibrating After the machine is
42. rogram and then press ENTER the system will show as Figure 7 9 and starts aging The default aging time is 48 hours You can adjust the aging time through Parameter Adjustment Aging Program washing 11 11 11 Figure 7 9 7 2 7 Packing Packing is a process of apparatus manufacture and is also used to place for a long time or made before line haul Press Packing and then press ENTER the system will show as Figure 7 10 and starts packing 51 Packing Program Please put duct into distilled water Packing please waiting 11 11 11 Figure 7 10 Packing Program Device washing please waiting Device washing please waiting 11 11 11 Figure 11 7 3 Malfunction Information Choose Malfunction Information in SERVICE it will show the malfunction saved in the system Correct password should be entered to carry out this command Mode Maint ain Status Setup Asa ist art Service Info Query djust Help Debug Power OFF Display Figure 7 12 Press Enter and show as figure 7 13 Malfunction Information List Press MENU to return the interface of counting 32 Malfunction Information Bottom Voltage Malfunction Information Display 11 11 11 Figure 7 13 Malfunction Information List 7 4 Parameter Calibrating The purpose of calibrating is to ensure the veracity of the test result Choose Parameter Calibrating in SERVICE to ente
43. s showed in Figure 7 4 Replace Diluent Replace Diluent Please Waiting Figure 7 4 7 2 2 Replace Hemolysin Choose Replace Hemolysin and then press ENTER the system will show as Figure 7 5 and start to replace hemolysin after finishing replacement the system will automatically return the interface as showed in Figure 7 5 4 3 4 3 1 45 2 Nal Ca GG Figure 5 7 2 3 Add Pre diluent Choose Add diluent and press ENTER as showed in Figure7 6 Then you put a sample cup under the sampling pin and press Start 1 6mL diluent will come out of the machine You can repeat the above steps to get several pre diluents Finally you can press MENU to return the interface as showed in Figure 7 6 49 Automatic Hematology Analyzer Fre dilute Idle v2 4 HO 00000000 ID 00000 WBC i 00 sp du LTH 0 vn khaa 0 ED Grant 3 0 1 LT 3 OO A bhig 0 be 5 100 150 2 0 ell H uran D I Hep gi REL D O0 e dl Hr 00 1 FL lt MEH 3 0 Py 50 100 150 200 250 H A8 ary A ROW 0 CH RD 4 H A PLT 0 10 e Maa J A H L L 0 5 10 15 2 25 F Ly 1 Ji E HEHU Measure Counting EHTER Demarcate 11 11 11 Figure 6 Note 1 Along time storage for the diluted sample may influence the counting veracity 2 Please keep the pr
44. t side Switch Light coupling Direct line slippery track Sampling pin sample needle module Installation Environment The environment should be clean without any dirt mechanic shake noise or any electronic disturbance Please keep the machine away from engine shining fluorescent lamp and other electronic equipments which are often turned on and off Don t put it in the sunshine or near the heat or wind Space Requirement The machine should be put on the experiment table which is big enough to place the reagents Notice Please place the reagents and machine on the same height Power Requirement The machine require power supply of AC 220VA the fluctuation of the voltage should not exceed 10 and the frequency should be 50 Hz Notice Make sure the input voltage is right and the right fuses are well installed The machine need a good grounded socket to provide power the biggest power is 150VA 2 3 Installation of the machine Bio pollution Samples reagents hematology controls standards and waste liquid may have danger of contagion So please wear protection clothes and glove and please obey the laboratory operating rules Warning The sampling pin is very acuate there may be sample hematology control and standard remained so there may be danger of contagion please donot touch the sampling pin Connect liquid path Diluent connection Take out the diluent pipe fr
45. t to the situation that won t disturb the analyses of WBC In this case the WBC can be classified into 3 part differential The Hemolysin can combine with hemoglobin to form a combination whose absorbance has nothing with the concentration of hemoglobin To get a best result PL 6A Hemolysin must be used with PL 6A Diluent Washing Solution This washing solution is an enzyme based isotonic and wetting agent It is used to clean pipeline and counting chamber This washing solution is the special solution for our product and will not harm the fluid lines Cleaner Used to maintain the instrument Calibrator lt is used to calibrate the instrument and quality control It is a commercial produced whole blood products used to monitor the performance of instrument The calibrator is divided into 3 kinds low normal high Use these 3 calibrator everyday to monitor the performance to ensure the accuracy of results Important Note XFAG6000ANI Animal Hematology Analyzer is only used for analysis of animal blood in vitro and no other specified uses Only allowed to use the special reagents that mentions in User s Manual or will not ensure the reliability of measuring data Read this User s Manual carefully before operating the apparatus and proper to preserve for future use the apparatus smells or smokes cut off power supply immediately and contact with the after sale engineer of our company if continue to use it it
46. ters will be non numeric Histogram alarm WBC histogram alarm Abnormal WBC histograms will be flagged by one of the markings R1 R2 R3 R4 and Rm R1 indicates abnormality on the left side of the lymphocyte hump and possible presence of platelet clumps giant platelets nucleated red cell insolvable red cell protein and lipoid debris in sample or electrical noise R2 indicates abnormality between the lymphocyte hump and the mid sized 28 cell area and possible presence of abnormal lymphocyte plasma cell atypical lymphocyte original granulocytes in the sample and eosinophilia or basophilia R3 indicates abnormality between the mid sized cell area and the granulocytes and possible presence of immature granulocytes abnormal sub population in the sample or eosinophilia R4 indicates abnormality on the right side of the granulocytes hump and netrophilia Rm indicates at least two R flags PLT Histogram Alarm Abnormal PLT histograms will be flagged by one of the markings P PS and PL Pm indicates blur demarcation between the platelet and red blood cell area and possible presence of large platelet platelet coagulation small red blood cell cell debris or fibrin Ps indicates excessive small PLIS PL indicates excessive large PLIS Adjust histogram manually If you are not satisfied with the obtained histograms you can adjust them manually In counting interface press ENTER to adjust histogram use t to
47. tient s No After testing the system will plus one to current No as the next No Notice lf the input sample No and the storage No are the same in history review it will not cover the content of the same Number In next counting sample number will plus one automatically if do not input number Background determination The instrument will make a background determination in startup clean and if current number is 0 In background determination the number is 0 and background range 15 as below the background determination result exceeds the range then the instrument will treat it as malfunction 3 4 Blood Sample Collection Bio pollution Samples reagents hematology controls standards and waste liquid may have danger of contagion So please wear protection clothes and glove and please obey the laboratory operating rules 24 Warning Avoid direct contact with blood samples of patients Operator should handle the blood sample carefully to avoid infection The operator must also wear Surgical gloves Anti coagulation of vein blood We recommend you to collect vein blood with a K2EDTA 1 5 to 2 2mg mL anticoagulant which can keep the WBC and RBC with the conformation stability in classifying measurement and counting Compounding of twig blood sample 1 Get into Pre dilute mode seen in Figure 3 5 lan WholeBlood Pre dilute Maint ain Paracerntesis Setup SeEVLCEe Qu
48. to SEX and press Enter and the default is male SEX can be shifted between male and female When the cursor moves to Enter press Enter to save the modification for the case and exit from Information Edit When the cursor moves to Cancel press Enter not to save the modification for the case and exit from Information Edit Chinese characters use Microsoft spelling to edit input Information Edit Sex Old NO Bedrum Section Sender Ckecker Accessor Enter Cancel Figure 6 5 List Review Press Menu to exit from the review 46 6 2 Sample Inquiry In Menu interface move the cursor to Search Sample Inquiry press to show the 3 choices ID Inquiry Name Inquiry and Date Inquiry please see Figure 6 6 Mode Maint ain Setup Service Review Query Query Ch Help Cc Power OFF Figure 6 6 Inquiry 6 2 1 ID Inquiry In MENU interface move the cursor to Search Sample Inquiry ID Inquiry to start ID inquiry the LCD will show as Figure 6 7 Sample Browse Average Ly PDY Start 0000 COUNT 00 Total Figure 6 7 Input the ID you want to inquiry in the blank then press ENTER the machine will start inquiry and display the result Chapter 7 Service There are some necessary service functions in the system to make it easier to operate The Service MENU includes system status malfunction
49. to start setup 4 Move the cursor to the item required to be set with 7 J 5 The items include Print Format Version Running Sequence Time Adjustment Gain Auto Cleaning Time Password and Reference Value 6 Press ENTER to start the selected item Search 1 Press MENU to start menu operation Move the cursor to Search with 1 J 58 3 Press to start searching 4 Move the cursor to the item required to be searched with 5 The items include Deblock Clean Drain chamber Drain pipeline Aging Valve Pump Detection Motor and Orifice Voltage 6 Press ENTER to start the selected item Operation 1 Preparation Check if the diluent washing solution and hemolysin is enough Check if the waste liquid barrel needs to be cleaned Check if the pipelines are flexed or if the electrical cables are well connected 2 Power on the machine it will automatically clean the pipelines and do self checking And then enter the counting interface after finishing the above jobs 3 Put the blood sample under the sampling pin and press START to start 4 After the pin is lifted take away the sample Each time 13ul sample will be sucked 5 About 1 minute later the counting result will be showed on the LCD Note While sucking the sample please keep the pin away from the sample cup otherwise the veracity will be influenced Mode 1 Press MENU to start menu operation Move t
50. uter Printer 36 Choose Inner Printer press PRINT the inner thermal recorder will print the result Choose Outer Printer Press PRINT and the outer printer will print the result Model of outer printer EPSON LQ 300K or LQ 1600K series Auto print On OT On In the counting interface the machine automatically prints the result after counting Off In the counting interface the machine does not automatically prints the result after counting 5 2 Version Print set Mode Version Maintain Sequence setup Time Service Gain Query Sraten Help Password Power OFF Reference Figure 5 3 The screen center will be listed as the version number when the version setup is chosen as Figure 5 4 If there are several versions in the system the user can use this function Press ENTER a dialog box will come out Please input password after the right password is input all the versions saved in this machine will be showed in the center of the screen Then just move the cursor to choose a version and press ENTER the system will start running the version you have chosen cal 1 1 seluk SEU 541 sulk WE 2 1 caloy ke ee Figure 5 4 5 3 Time Setup 37 In the MENU interface move the cursor to Print set Mode Verzion Maintain sequence Setup Time Service Gan query Syatem Help Password Power OFF Figure 5 5 ENTE
51. will cause fire get an electric shock or personnel injured if the liquid flows into apparatus or the apparatus leaks cut off the power supply immediately and contact with the after sale engineer of our company If continue to use it it will cause fire get an electric shock or personnel injured Not block the intake back of the machine shell which is used to ventilate to ensure the reliable run of the apparatus In order for your safety and the reliable run of the apparatus the power supply used in this machine must have good grounding line Please follow the health and safe standard of the nation and it includes but not limited that equipped with protective eye patch gloves and fit experiment clothes when operating or maintaining this apparatus Safe Symbol The manual will hint these symbols in the essential place in order to warn for the potential danger APD sictoay danger show that the biology danger exists If ignore the signal or operate the machine by bg Warning mistake it will cause the death of operators injured seriously If ignore the signal or operate the machine by bg Note mistake it will cause the injury for operators or the damage of apparatus performance The information that you need to know to keep Important the performance of the machine Complementary material Our company will send the complementary material including operation and maintenance about this machine aperiodic
52. ws operation Press MENU to operate Screen saver This instrument has the function of screen saver if no operation within 10 minutes the screen will be turned off automatically press any key to quit previous display When in screen saver status the sampling pin moves to RBC counting chamber when quit this status the sampling pin will back to ready status MENU Mode WholelBlood Deblock Clean D Chamber Diluent Chamber e system TT Wash MotorCheck Password Ei Wash Aging Burn Male Motor K Child Figure 1 11 Chapter 2 Installation 2 1 Package Please check the package carefully and also check the appearance of the machine 16 Please let us know immediately if there is breakage during transportation Please check the machine against the packing list to make sure that you have received all the accessories Please contact us immediately if you find any missed accessory 2 2 Installation Requirement The motor M2 and the swivel plate is fixed by nylon strip to prevent the sampling assembly from moving during the transportation You must it when you receive the machine Detail as below 1 Push the switch according to the arrow direction in below figure to open the right side of the machine 2 Find the nylon strip between the sampling assembly and motor M2 3 Nip the nylon strip with scissor 4 Connect the machine to diluent hemolysis and the waste bottle through tube Then power on and test righ
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