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Leica Z6 APO A Leica Z16 APO A User Manual

1. Y tube SLA G 79 DII O76 User Manual Leica Z6 APO A Z16 APO A Video Phototube A M38x1 A Interface for Video Photo Objectives 4 25 5 O87 r Interface for Zoom Coaxial Illuminator etc Interface for Microscope Carriers Leica Z6 APO A Z16 APO A User Manual 92 Microscope carrier Carrier for AS tube Leica Z6 APO A Z16 APO A User Manual 93 Focusing arm for probers 43 UOlISOd Buisn904 IseubiH Lowest So8SJU SUIYDEN 104 PIN SAJOY GPO Xp 289 eoejJoju User Manual Leica Z6 APO A Z16 APO A Focusing arms Highest a dia Focusing a Positio ae Highest Focusing Position o 101 121 in o 25 Co A O o Ar a hd i po 915 8 q E To E lj a Lowest E Focusing So Position S a 148 E 5 RICH I nl S BR Interface Interface for 25 Column i Focusing arm for bonders Tiltable focusing arm Leica Z6 APO A Z16 APO A User Manual 95 Adapters 45 mm OAS M42 Fine Focus Interface M42 Zoom Fine Focus Interface 40 mm 7 5 max M25x0 75 DM
2. Increased contrast without relief is attained if both diaphragms are set to 45 A gap like illu minated area is created By tilting the deflection mirror slightly you can move the gap over the entire field of view and quickly toggle between positive and negative relief images The dyna mic effect makes it easy to distinguish phase structures from amplitude structures 72 Leica TL RCI Methods in Transmitted Light Vertical bright field illumination Suitable for stained amplitude specimens with sufficient contrast The light beams are deflected vertically through the specimen This results in an accurate bright field with maximum brightness Leica Z6 APO A Z16 APO A Inclined transmitted light Suitable for semitransparent opaque speci mens such as foraminifera and fish eggs Move the deflection mirror until the desired data are visible User Manual Single sided dark field Suitable for fixed specimens and fine structu res The flatter the angle at which the light beams are deflected into the specimen plane the darker the substrate appears A dark field like transmitted light is created Outlines fine edges and structures are bright in contrast with the dark background through diffraction of the light beams on the dark background Leica TL RCI Relief Images Starting position Positive relief contrast Negative relief contrast Suitable for semitransparent and transparent Suitable for semitrans
3. Leica Z6 APO Leica 216 APO A User Manual MICROSYSTEMS General Instructions Safety Concept Use in clean rooms Servicing Before using your macroscope for the first time The Leica Z series can be used in clean rooms x Repairs may only be carried out by Leica please read the Safety Concept brochure without any problems Microsystems trained service technicians included with your instrument It contains addi Only original Leica Microsystems spare tional information about handling and care Cleaning parts may be used Do not use any unsuitable cleaning agents chemicals or techniques for cleaning E Bezpe nostn koncepce Sikkerhedskoncept Sicherheitskonzept Ohutuseeskiri Safety concept Concepto de seguridad Concept de s curit Concetto di sicurezza Drosibas noteikumi Responsibilities of person in charge of Never use chemicals to clean colored instrument surfaces or accessories with rubberized Ensure that the Leica macroscope is opera Saugos samprata parts This could damage the surfaces and ted maintained and repaired by authorized Biztons gi el ir so L astako ehi specimens could be contaminated by abra and trained personnel only Koncepcja nn ser ded particles Varnostni koncept Turvallisuus S kerhetskoncept Leica In most cases we can provide special solu BEE cases AMT DABA tions on request Some products can be modified and we
4. Constant Color Intensity Control No Internal shutter lamp control No Integrated filter holder Yes Coated optics for increasing the color Yes temperature Matching of high num aperture No Remote control options No AntiShock Pads Yes Dimensions WxHxD 340x430x85 mm Leica Z6 APO A Z16 APO A User Manual 105 Leica TL BFDF Transmitted light Base Light source External via cold light source Illuminated area 40 mm Connections Connection for cold light guide active f 10mm end tube f 13mm Weight Illumination types 5 8 kg Bright field Yes Dark field Yes Oblique light No Relief Contrast System RC No CCIC Constant Color Intensity Control No Internal shutter lamp control Yes Integrated filter holder No Coated optics for increasing the color No temperature Matching of high num aperture Yes Remote control options Yes AntiShock Pads Yes Dimensions WxHxD 340x390x90 mm With cold light source Leica CLS150 LS Leica Z6 APO A Z16 APO A Concave mirror With external light source User Manual 106 Leica TL RC TL RCI Leica TLRC Leica TL RCI Light source External via cold light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area Power supply unit 35 mm 35 mm 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 1
5. Installing the Leica LED3000 NVI Connect the Leica LED3000 NVI to the focusing column via the CTL2 connection if the focusing column is equipped with inte grated electronics 1 User Manual Alternative installation for manual columns 1 Connect the external power supply unit 10 450 266 to the Leica LED3000 NVI Place the Leica LED3000 NVI on the objec tive from below and tighten the locking screw 46 Quick Start Guide Overview of an automated Leica Z Series Macroscope Leica Z16 APO A Digital camera Photo videotube Eyepieces Video Phototube Y Microscope carrier Connection for control cable Optics carrier zoom body Objective LED ring illuminator IsoPro motorized mechanical stage 11 SmartTouch control unit 12 Motorized microscope column Oo DAN ANH da LUNS So Leica Z6 APO A Z16 APO A User Manual 48 Eyepieces Leica Z6 APO A Z16 APO A User Manual 49 Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the macro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The following eyepieces are available for the Z series Magnification Dioptric Correction Order number 10x 5 diopter settings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 026
6. Optical Data Visual with Y Tube 1 25x Objectives 1x planapochromatic 0 5x planapochromatic 0 8x planapochromatic 2x planapochromatic 5x planapochromatic Working distances 97 mm 187 mm 112 mm 39 mm 20 mm Zoom Total Object Total Object Total Object Total Object Total Object Eyepieces position mag field mag field mag field mag field mag field x mm x mm x mm x mm x mm 0 57 7 1 32 3 3 6 64 6 5 7 40 4 14 3 16 1 35 6 6 5 0 8 10 23 0 5 46 0 8 28 8 20 11 5 50 4 6 1 12 5 18 4 6 3 36 8 10 23 0 25 9 2 62 5 3 7 1 25 15 6 14 7 7 8 29 4 12 5 18 4 31 3 7 4 78 1 2 9 1 6 20 11 5 10 23 0 16 14 4 40 5 8 100 2 3 2 25 9 2 12 5 18 4 20 11 5 50 4 6 125 1 8 2 5 31 3 7 4 15 6 14 7 25 9 2 62 5 3 7 156 3 1 5 1020235 3 2 40 5 8 20 11 5 32 7 2 80 2 9 200 1 2 3 6 45 5 1 22 5 10 2 36 6 4 90 2 6 225 1 0 4 50 4 6 25 9 2 40 5 8 100 2 3 250 0 9 5 62 5 3 7 31 3 7 4 50 4 6 125 1 8 312 5 0 7 6 3 78 8 2 9 39 4 5 8 63 3 7 157 5 1 5 393 8 0 6 8 100 2 3 50 4 6 80 2 9 200 1 2 500 0 5 9 2 115 2 0 57 5 4 0 92 2 5 230 1 0 575 0 4 Leica Z6 APO A Z16 APO A User Manual 102 Optical Data Visual with Y Tube 1 25x cont d Objectives 1x planapochromatic 0 5x planapochromatic 0 8x planapochromatic 2x planapochromatic 5x planapochromatic Working distances 97 mm 187 mm 112 mm 39 mm 20 mm Zoom Total Object Total Object Total Object Total Object Total Object Eyepieces position mag field mag field mag field mag field
7. DFC500 0 63x 0 8x User Manual Cameras from third party suppliers In addition to Leica DFC cameras with the stan dardized C mount interfaces you can connect third party cameras such as single lens reflex cameras to the macroscope using a T2 bayonet adapter To do so instead of the C mount adap ter simply use the corresponding SLR adapter with T2 connection However these third party cameras are not integrated into the Leica Appli cation Suite and have to be operated using the corresponding software from the camera manufacturer The Leica digital cameras are detailed in a sepa rate user manual along with instructions for their assembly and use 58 Trinocular Video Phototube 50 Use With its third beam path the trinocular video phototube 50 enables you to simultaneously view and photograph a specimen The available light is divided as follows k 50 is available for the two eyepieces 50 of the light is diverted to the video photo beam path Assembly Fasten the trinocular tube 50 to the optics carrier instead of the ErgoTube refer also to page 27 Leica Z6 APO A Z16 APO A User Manual 59 Trinocular Video Phototube 100 Use Switchover With its third beam path the trinocular video Turn the controller on the right side of the phototube 100 enables you to either view or tube into the horizontal position in order to photograph a specimen This means that 100 guide all available
8. Insert the glass plate back into the standard stage User Manual 32 IsoPro Manual Mechanical Stage Assembly Leica IsoPro Mechanical Stage Before the Leica IsoPro mechanical stage is mounted to the base the axis containing the control buttons is attached either on the left or the right side of the mechanical stage If the controls are to be mounted on the left hand side the gear rod on the bottom side of the mechanical stage must be unscrewed and reattached in reverse 1 Take the glass plate from the mechanical stage 2 Turn the mechanical stage around and place it onto a non slip surface 3 Change the gear rod from the left to the right hand side 4 Skip the next two steps to install the cont rols Leica Z6 APO A Z16 APO A Before the Leica IsoPro mechanical stage is mounted to the base the axis containing the control buttons is attached either on the left or the right side of the mechanical stage User Manual 33 Leica lsoPro Manual Mechanical Stage Assembly cont d Left or right operation If the controls are to be mounted on the left hand side the gear rod on the bottom side of the mechanical stage must be unscrewed and reattached in reverse 1 Take the glass plate from the mechanical stage and turn it around 2 Change the gear rod from the left to the right hand side Leica Z6 APO A Z16 APO A Control assembly 1 Take the glass plate from the mechanical
9. Z16 APO A The Leica SmartTouch allows you to H control and save all automatic functions and reload them if required This gives you maximum assurance for your experiments since you can reproduce every situation quickly and easily For additional information about the Leica SmartTouch and operation refer to the User Manual provided with the instrument User Manual 83 Manual Control Focusing Turning counterclockwise downwards Turning clockwise upwards Toggle between coarse and fine focusing 1 Press the black button briefly A When the system is switched on the coarse focus is always switched on also Leica Z6 APO A Z16 APO A Information about the focus positions x x Focus positions can be stored using the manual control or on the computer A total of up to 5 focus positions can be stored If a sixth focus position is stored the one nearest to it will be deleted Each focus position can be canceled indivi dually The system will travel to each of the indi vidual focus positions in the sequence in which they were stored Switching off the system cancels all of the stored focus positions User Manual Saving the focus positions 1 2 Focus on the first specimen detail Press the red switch for at least 1 5 seconds Short beeps provide confirmation Focus on the next specimen details and save these positions also Moving to stored focus positions 1 Press the re
10. in the X direction as otherwise the mechanical system will be damaged 1 Place the mechanical stage onto the base 2 Pull the upper part of the mechanical stage carefully towards the user and fasten the lower part onto the transmitted light base 3 Attach the mechanical stage evenly to the three threaded holes 4 Now move the mechanical stage as far as it will go in the direction of the column 5 Insert the glass plate into the mechanical stage User Manual 38 Leica IsoPro Motorized Mechanical Stage Assembly cont d Mechanical stage for X Y stage control As soon as all instruments have been plugged module into the control box and it has been plugged 1 Plug the CTL2 plug of the mechanical stage into the power supply the mechanical stage motor into one of the three available CTL2 initializes and automatically drives to the mid interfaces position 2 Plug the CTL2 plug of the Leica PSC control ler into another CTL2 interface The third CTL2 interface is available for connecting other instruments such as the Leica foot switch 10 447 398 or TL RCI transmitted light base 10 446 352 4 Connect the PC where applicable and X Y Stage DCI Module using a suitable USB cable k The 15 pin Sub D interface is intended for use with the Leica SmartMove controller 11 501 197 3 Plug a power cable into the socket provided and into a grounded power socket Leica Z6 APO A Z16 APO A User Manu
11. mag field x mm x mm x mm x mm x mm 0 57 11 4 21 1 5 7 42 1 9 1 26 3 22 8 10 5 57 4 2 0 8 16 15 0 8 30 0 12 8 18 8 32 7 5 80 3 0 1 20 12 0 10 24 0 16 15 0 40 6 0 100 2 4 1 25 25 9 6 12 5 19 2 20 12 0 50 4 8 125 1 9 1 6 32 7 5 16 15 0 25 6 9 4 64 3 8 160 1 5 2 40 6 0 20 12 0 32 7 5 80 3 0 200 1 2 2 5 50 4 8 25 9 6 40 6 0 100 2 4 250 1 0 lex 420 3 2 64 3 8 32 7 5 51 2 4 7 128 1 9 320 0 8 3 6 72 3 3 36 6 7 57 6 4 2 144 17 360 0 7 4 80 3 0 40 6 0 64 3 8 160 1 5 400 0 6 5 100 2 4 50 4 8 80 3 0 200 1 2 500 0 5 6 3 126 1 9 63 3 8 100 8 2 4 252 1 0 630 0 4 8 160 1 5 80 3 0 128 1 9 320 0 8 800 0 3 9 2 184 1 3 92 2 6 147 2 1 6 368 0 7 920 0 3 0 57 17 8 13 3 8 91 26 7 14 3 16 7 35 6 6 7 89 2 7 0 8 25 9 5 12 5 19 0 20 11 9 50 4 8 125 1 9 1 31 3 7 6 15 6 15 2 25 9 5 62 5 3 8 156 1 5 1 25 39 1 6 1 19 5 12 2 31 3 7 6 78 1 3 0 195 1 2 1 6 50 4 8 25 9 5 40 5 9 100 24 250 1 0 2 62 5 3 8 313 7 6 50 4 8 125 1 9 313 0 8 2 5 78 1 3 0 39 1 6 1 62 5 3 8 156 1 5 391 0 6 25058 3 2 100 2 4 50 4 8 80 3 0 200 1 2 500 0 5 3 6 113 2 1 56 3 4 2 90 2 6 225 1 1 562 0 4 4 125 1 9 62 5 3 8 100 2 4 250 1 0 625 0 4 5 156 1 5 78 1 3 4 125 1 9 313 0 8 781 0 3 6 3 197 1 2 98 4 2 4 158 1 5 394 0 6 984 0 2 8 250 1 0 125 1 9 200 1 2 500 0 5 1250 0 2 9 2 288 0 8 144 1 6 230 1 0 575 0 4 1438 0 2 Leica Z6 APO A Z16 APO A User Manual 103 Optical Data Visual with Y Tube 1 25x cont d Objectives 1x planapochromatic 0 5x pl
12. men Only the structure of semitransparent opaque objects disperses the light making the object visible against a dark background Turn the control as far as it will go towards DF dark field Fingertip with bright field illumination User Manual Identical subject with dark field illumination Leica TL RC TL RCI Controls Vertical column transmitted light base adapter plate 1 Power switch l l 2 Power connection socket sd i the AE halogen 3 USB socket type B GR only TL ne 4 USB socket type A Extension plate for focusing drives 5 2CANbus Standard stage 10 447 269 6 Screws for changing the halogen lamp Filter holder Control of top and bottom flaps of the Rottermann Contrast 6 Button for mirror and horizontal movement of the mirror 7 Transmitted light base GU WR WwW KN Leica Z6 APO A Z16 APO A User Manual 69 Leica TL RCI The Deflection Mirror Inverted world Depending on the properties of the specimen refractive index of the environment and the perception of the viewer it is possible that the switches described below for positive and inver ted relief contrast are to be operated in reverse This means that the lower switch rather than the upper switch controls the inverted relief contrast and vice versa Leica Z6 APO A Z16 APO A Tilted mirror The built in mirror features one flat and one concave side and can be rotated and moved The concave side has b
13. nation must be installed on the planapo chromatic objectives Leica Z6 APO A Z16 APO A Installing the coaxial illuminator 1 Guide the coaxial illuminator to the tube from the left or right 2 Adjust the coax illuminator so that the port for the light guide faces the direction that is the most comfortable for your work User Manual 3 Push the coaxial illuminator upwards and tighten the screw on the coaxial illumina tor 4 Connect the light guide of the Leica cold light source to the coaxial illuminator A The User Manual for the Leica cold light source is provided with the cold light source 24 Optics Carrier Tools used 1 Guide the optics carrier over the tube from 2 Pushthe optics carrier upwards and tighten Allen key provided the rear the fastening screw The instructions on this page also apply logically if the optional coaxial illumi nator has been installed see page 24 In this case the optics carrier is attached to the coaxial illuminator instead of the tube Assembling the optics carrier Leica Z6 APO A Z16 APO A User Manual 25 Objective Tools used You can attach the quarter wave plate for coaxial illumination the analyzer for polarization or a ring illuminator to the plana pochromatic objectives HR and DM objectives are high perfor mance objectives and always cause vignetting This vignetting is not a malfunction Leica Z6 APO A Z16 APO A Attaching the ob
14. series macroscopes In addition to capturing images it lets you control the macroscope illu minator stages cameras and more For additio nal information refer to the LAS online help 57 Photo Tubes and C mounts Application All Leica DFC cameras are equipped with a standardized C mount interface In turn the C mount adapter for the respective trinocular tube is connected to this interface This adapter creates a solid mechanical connection between the macroscope and camera and ensures opti mum rendering of the macroscopic image on the image sensor of the camera Usually the ideal is for the digital camera to capture as much of the field of vision as possi ble while excluding as much of the black edge of the field of vision as possible To do so the magnification factor of the C mount adapter must match the image format of the sensor as closely as possible see table If there is unwanted shading at the corners even with a compatible C mount adapter installed it can be corrected using the Shading function of the camera software Leica Z6 APO A Z16 APO A Alternatively you can also use a C mount adap ter with higher magnification This primarily avoids the critical border area of the field of vision and concentrates on the center of the field of vision Camera optimal suitable large smaller image field image field DFC295 0 5x 0 63x DFC420 0 5x 0 63x DFC490 0 63x 0 8x
15. the potentio meter 2 Click again to switch on the illumination The electronics returns the color tempera ture to the previously configured settings User Manual Using a USB mouse only TL RCI The Leica USB mouse controls the CCIC and dimming function of the TL RCI base Connect the mouse to the corresponding USB port of the base The scroll wheel of the mouse is occupied by default with the CCIC control system and is used to control the illumination intensity To switch the illumination on or off briefly click the scroll wheel To enter or exit dimming mode click and hold down the scroll wheel for longer than 2 seconds 71 Leica TL RC TL RCI Operation Setting the relief contrast The two switches on the left side of the trans mitted light base TL RC TL RCI activate two built in flaps The outer switch 1 controls the inverted relief contrast and the inner switch 2 adjusts the positive relief contrast 1 Switch for adjusting the inverted relief cont rast 2 Switch for adjusting the positive relief cont rast 3 Deflection mirror Leica Z6 APOA Z16APOA Depending on the flap position a part of the opening of the built in Fresnel lenses are covered which results in the different contrast effects Phase structures typically act as spatial relief type images in the positive relief cont rast like hills in the inverted relief contrast like valleys User Manual
16. 0 40 C Connections Connection for cold light guide active f 10mm end tube f 13mm 1xUSB type A 1xUSB type B 2xCAN BUS Weight 6 0 kg 7 2 Kg Illumination types Bright field dark field Yes yes Yes yes Oblique light Relief Contrast System RC Yes yes Yes yes CCIC Constant Color Intensity Control No Yes Internal shutter lamp control Yes Yes Integrated filter holder Yes Yes Coated optics for increasing the color temperature Yes Yes Matching of high num aperture Yes Yes Remote control options Yes Yes AntiShock Pads Yes Yes Dimensions of base WxHxD 340x390x95 mm 340x440x95 mm Single sided With cold light source Leica CLS150 LS Concave mirror With external light source Leica Z6 APOA Z16APOA User Manual 107 Leica IsoPro Motorized XY Stage Compatibility Leica incident light and TL bases TL BFDF TL RC TL RCI Stage size LxWxH 335 5 mm x 370mm x 41 5 mm Travel 152mm x 102mm 6 x 4 Speed X Y 30mm s Motor resolution X Y 0 25 um Repeatability X Y 2 um Positioning accuracy X Y 20 um over the entire positioning range Hysteresis X Y 30 um Motor Direct current DC motor with encoder Backlash Slack free Adapters All adapters for TL bases Acceleration S shaped acceleration Computer connection Control options USB Leica PSC Leica Smart Move Leica LAS via PC Powe
17. 1 Unscrew the extension plate from the base installed Make sure the instruments are unpa using the Allen key provided cked on a flat sufficiently dimensioned and non slip surface Leica Z6 APO A Z16 APO A User Manual Attach your focusing drive column from below using the four hexagon head screws Re attach the adapter plate to its origi nal position using the six hexagon head screws 29 Transmitted light Base TL BFDF Before First Use Removing the transport anchors Before you can use the transmitted light base for the first time it is absolutely necessary to remove the two transport anchors Anchor of mirror Anchor of switching slide Leica Z6 APO A Z16 APO A User Manual 30 Transmitted light Base TL BFDF Standard delivery The base is delivered with the adapter plate installed The selected stage Leica IsoPro mechanical stage or Leica standard stage 10 447 269 and the focusing drive will have to be mounted later Stage assembly The Leica TL BFDF transmitted light base can be equipped with three different stages k Leica IsoPro manual mechanical stage Leica IsoPro automatic mechanical stage k Leica standard stage 10 447 269 Leica Z6 APO A Z16 APO A The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disasse
18. ILLIE 43 Cables Diagram 2 3 26 16 APO amp APO A with interface on HighPerformance M Series Column Z6 Z16 APO A Z Series DCI Box Interface on Focus Column e e CTL2 Ports O e Do not use CTL1 Port and USB q g emeena Power Plug when DCI Box is connected to the motor focus column A O Power Supply o Pe i Pira is Serial connection loop possible Serial connection of up to 5 footswitches possible For motorfocus only Not with new motorfocus an gt KKK SmartTouch Footswitch Handwheel UMC 10 450 298 Leica Z6 APO A Z16 APOA User Manual 44 Cables Diagram 3 3 26 Z16 APO amp APO with TL RCI Base and IsoPro Stage PC a Do not use CTL1 Port and Z Series Power Plug when DCI Box DCI Box is connected to the motor focus column Interface on Focus Column e e gi USE DCI Controlbox Z6 Z16APO A A O Power Supply sp OO Alternative installation e Please use the Leica Application Suite to configure the functions of the system LED3000 NVI SmartTouch Smart Move PSC Connector from IsoPro Stage Leica Z6 APO A Z16 APO A User Manual 45 Leica LED3000 NVI Near Vertical Illumination Required tools None The Leica LED 3000 NVI can be used with any objectives that have an outer diameter of 58 mm The supported working distance is between 50 mm and 150 mm Leica Z6 APO A Z16 APO A
19. Leica Z6 APO A Z16 APO A User Manual 50 Using the Eyepieces The eyepieces form the connection between If you do not wear glasses If you wear glasses the tube and the eye of the observer Simply 1 Hold the eyepiece firmly and rotate the 1 Hold the eyepiece firmly and rotate the push them into the tube and they are ready to eyecups forwards counterclockwise eyecups clockwise towards the rear as use otherwise the viewing distance is too great Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens 2 If an eyepiece is equipped with the integ rated dioptric correction turn the value to 2 If an eyepiece is equipped with the integ the 0 mark rated dioptric correction turn the value to the 0 mark Dioptric Correction A built in dioptric correction is available for eyeglass wearers For more information refer to Page 51 By the way one benefit of viewing with eyeglas ses is a drastically lower risk of bacterial trans mission see page 53 The soft material of the eyecup also ensures that your glasses will not be scratched even if they contact the eyepiece Leica Z6 APO A Z16 APO A User Manual 51 The Correct Interpupillary Distance The interpupillary distance is correctly set if you Adjusting the interpupillary dista
20. Objective Interface M60 Achromat Objective Interface O37 66 mm Adapter for achromatic objectives Adapter for DM objectives User Manual 96 Leica Z6 APO A Z16 APO A Coaxial incident light housing Leica Z6 APO A Z16 APO A User Manual 97 Specifications Leica Z6 APO A amp Z16 APO A Leica Z6 APO A Leica Z16 APO A Zoom 6 3 1 motorized 16 1 motorized Zoom factor 0 57x 3 6x 0 57x 9 2x Built in iris diaphragm Y motorized Y motorized Engageable zoom positions Continuously motorized and approachable positions Continuously motorized and approach able positions Zoom speed 1 6 s for zoom range 2 5 s for zoom range Visual data with 1x planapochromati c objective 10x eyepieces 1 25x Y tube Magnification 7 1X 45x 7 1x 115x Resolution 60 351 Lp mm 51 336 Lp mm Visible structural width 8 3 1 4 um 9 8 1 49 um Numerical Aperture 0 02 0 117 nA 0 017 0 112 nA Object field 29 5 mm 4 7 mm 29 5 mm 1 83 mm Depth of field diaphragm open 3 1 mm 0 09 mm 3 8 mm 0 05 mm Depth of field dia closed 18 1 mm 0 4 mm 18 4 mm 0 4 mm Visual data with 2x planapochromati c objective 40x eyepieces 1 25x Y tube Magnification 57x 360x 57x 920x Resolution 120 702 Lp mm 102 672 Lp mm Visible structural
21. The selected stage LeicalsoPro mechanicalstageorstandardstage 10 447 269 and the focusing drive will have to be mounted later The motorized mechanical stage is a sensitive precision instrument During installation avoid subjecting the stage to impact or severe vibra tions 1 Microscope base 3x M4 screws 2 Motorized mechanical stage Leica Z6 APO A Z16 APO A User Manual 36 Leica IsoPro Motorized Mechanical Stage Assembly cont d 3 Remove the two M3 screws and 4 Remove the two M4 screws and the sleeve 5 Remove the four shock absorbing cartons the holder from the mechanical stage from the mechanical stage from the mechanical stage A After removal keep all the transport anchors in the plastic bag provided for future transport Leica Z6 APO A Z16 APO A User Manual 37 Leica lsoPro Motorized Mechanical Stage Assembly cont d The bases The TL BFDF TL RC and RCI transmitted light bases can be equipped with three different stages Standard stage manual and automated IsoPro mechanical stage The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica Z6 APO A Z16 APO A Mechanical stage and base Never move the sledge of the motorized mechanical stage manually
22. Tools used the three threads at the end of the column Allen key provided protrude over the edge H Tip The base can be installed more easily if someone helps you do so Leica Z6 APO A Z16 APO A User Manual 18 Transmitted light Bases and Manual Focusing Column For additional information on the TL Assembling the column adapter Assembling the focusing drive bases refer to page 29 1 Securely install the column adapter on the 2 Securely screw the focusing column to the column using the four included screws base using the six included screws The first step is to connect the focusing column to the corresponding base Tools used Allen key provided Leica Z6 APO A Z16 APO A User Manual 19 Microscope Carrier Tools used Assembling the Microscope Carrier Allen key provided 1 Press the microscope carrier against the 2 Tighten the screw using the Allen key provi focusing column ded Leica Z6 APO A Z16 APO A User Manual 20 Video Phototube A The video phototube A is used if only a 2 Insertthe tube into the microscope carrier Installing the objective camera Leica digital camera with the Leica LAS 1 Screw the desired digital camera to the Leica Application Suite software is used to video objective observe the specimen Assembling the tube 1 Unscrew the locking screw on the optics carrier 3 Tighten the locking screw on the micro scope carrier 2 Insert the video objective into the vid
23. a Application Suite LAS or the Leica SmartTouch The supported working distance is between 50 mm and 150 mm Leica Z6 APO A Z16 APO A Use The light of the Leica LED3000 NVI can be very bright Therefore always switch on the illuminator before you look through the eyepieces 1 Switch on the illuminator by briefly pres sing the ON OFF button The green LED on the upper left corner now lights up User Manual 2 Adjust the brightness by briefly pressing the O or buttons 3 Switch off the illuminator by briefly pres sing the button Accessories Leica PSC Controller Use The Leica PSC controller gives you precision control of the Leica IsoPro motorized mecha nical stage Connection Connect the Leica PSC controller and the Leica IsoPro motorized mechanical stage to the DCI box Leica Z6 APO A Z16 APO A Passing over the specimen quickly Move the joystick in any desired direc tion including diagonally to control the mechanical stage quickly Fine control of the mechanical stage The motorized Leica IsoPro mechanical stage offers an accuracy of up to 0 25um To move to a position with maximum accuracy use the left and right knobs on the joystick User Manual Control by LAS The instructions for controlling the Leica IsoPro using the Leica Application Suite are provided in the software s help file 82 Leica SmartTouch Leica Z6 APO A
24. a danger This symbol indicates especially impor A tant information that must be read and complied with Failure to comply can cause the following k Hazards to personnel Functional disturbances or damaged inst ruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following k Hazards to personnel Functional disturbances or damaged inst ruments Leica Z6 APO A Z16 APO A Danger due to hot surface This symbol warns against touching A accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide clarity Explanatory notes This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Safety Instructions Description The individual modules fulfill the highest requirements for observation and docu mentation with the Leica Z series Intended use Refer to Safety Concept booklet Non intended use Refer to Safety Concept booklet Never use Z series macroscopes or their compo nents for surgical procedures for example on the eye unless they are specifically intended for that purpose The instruments and accessories descri
25. al 39 Cables Connections The A version of the Leica Z series features extensive automation options with which vari ous microscope data and settings can be read out transferred to the PC and reproduced later Leica Z6 APO A Z16 APO A The terminals User Manual The connection to the PC and to other instru ments is made using the terminals on the rear side of the column 1 2x CTL2 terminals for auxiliary equipment such as the distribution box of the Z series the SmartTouch panel the Leica TL RCI base and other accessories from the Leica product range 2 Terminal for the power supply provided 3 USB terminal for the connection to the PC 40 Cables Cable Duct The integrated cable duct in the column enab Feeding the cables 3 Place the cables in the cable duct and screw les a neat cable layout around the macroscope 1 Unscrew the three screws on the cable the cover on tightly For example the USB or FireWire cables of the duct camera can be stowed in the cable duct 2 Remove the cover of the cable duct Tip Estimate the length of the cable ends you will need before screwing on the cover For thick cables it is difficult to change the length retroactively Leica Z6 APO A Z16 APO A User Manual 41 Motorized Focus Adjusting the Travel Path Depending on the work situation it is H necessary to restrict the maximum travel path of the macroscope This prevents the following Injuries w
26. alities you associate with the name Leica Microsystems excellent objectives high quality engineering and reliability Furthermore the modular design ensures that the Z series adapts perfectly to your needs no matter which accessories you require for your tasks Thanks to the parallax error free observation measurements are more accurate and adaptation tasks are easier Together with a Leica digital camera you obtain the maximum amount of high quality data which also makes later evaluations on the computer much easier Leica Z6 APO A Z16 APO A Though the reliability and robustness of Leica macroscopes is legendary like any high tech product the Leica Z series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the information you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your Leica macroscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work after all you are now equipped with the best tool User Manual 13 The Modular Design Everything is Relative The Leica Z series provides maximum flexibility in choosing equipment thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be comb
27. anapochromatic 0 8x planapochromatic 2x planapochromatic 5x planapochromatic Working distances 97 mm 187 mm 112 mm 39 mm 20 mm Zoom Total Object Total Object Total Object Total Object Total Object quee position mag field mag field mag field mag field mag field x mm x mm x mm x mm x mm 0 57 28 5 8 4 14 3 16 8 22 8 10 5 57 4 2 143 1 7 0 8 40 6 0 20 12 0 32 7 5 80 3 0 200 1 2 1 50 4 8 25 9 6 40 6 0 100 2 4 250 1 0 1 25 62 5 3 8 31 3 7 7 50 4 8 125 1 9 313 0 8 1 6 80 3 0 40 6 0 64 3 8 160 1 5 400 0 6 2 100 2 4 50 4 8 80 3 0 200 1 2 500 0 5 2 5 125 1 9 62 5 3 8 100 2 4 250 1 0 625 0 4 40x 6B 3 2 160 1 5 80 3 0 128 1 9 320 0 8 800 0 3 3 6 180 1 3 90 2 7 144 1 7 360 0 7 900 0 3 4 200 1 2 100 2 4 160 1 5 400 0 6 1000 0 2 5 250 1 0 125 1 9 200 1 2 500 0 5 1250 0 2 6 3 315 0 8 158 1 5 252 1 0 630 0 4 1575 0 2 8 400 0 6 200 1 2 320 0 8 800 0 3 2000 0 1 9 2 460 0 5 230 1 0 368 0 7 920 0 3 2300 0 1 Leica Z6 APO A Z16 APO A User Manual 104 Leica TL ST Transmitted light Base Light source Halogen lamp 12V 20W Quick illuminant change Yes Illuminated area 50mm Power supply unit Input voltage 100 240 V frequency 50 60 Hz energy consumption 30 W max ambient temperature 10 40 C Connections Power plug power switch Weight 7 4 kg Illumination types Bright field Yes Dark field Yes single sided Oblique light No Relief Contrast System RC No CCIC
28. bed in this operating manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Leica Z6 APO A Z16 APO A Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims Place of use Refer to Safety Concept booklet Electrical components must be placed at least 10 cm away from the wall and from flammable substances Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort measurements and micro graphic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus User Manual Responsibilities of person in charge of instrument Refer to Safety Concept booklet Ensure that k The Z series macroscopes and accessories are operated maintained and repaired by authorized and trained personnel only All operators have read understood and observe this User Manual and particularly the safety regulations Repairs service work Refer to Safety Concept booklet Only original Leica Microsystems spare parts may be used Before opening the instruments switch off the power and unplug the power cable Touching the live circuit can cause injury Safety Inst
29. can offer other accesso ries for use in clean rooms Leica Z6 APO A Z16 APO A User Manual Important Safety Notes User Manual The individual modules of the Leica Z series include an interactive CD ROM with all relevant user manuals in other languages Keep it in a safe place and readily accessible to the user User manuals and updates are also available for you to download and print from our web site www leica microsystems com This User Manual describes the special func tions of the individual modules of the Leica Z series and contains important instructions for their operational safety maintenance and accessories The Safety Concept booklet contains additi onal safety information regarding the service work requirements and the handling of the macroscope accessories and electrical accesso ries as well as general safety instructions Leica Z6 APO A Z16 APO A You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the user manual and the safety requirements of the supplier Before installing operating or using the instru ments it is mandatory to read the user manuals listed above In particular please observe all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals User Manual Symbols Used Warning of
30. cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Move the slider backwards until the desired effect is achieved Inclined transmitted light Transmitted light that traverses the object obli quely will provide additional resolution and information when observing semitransparent opaque objects Slowly pull the slider towards yourself until the desired effect is achieved 65 Leica TL ST Transmitted light Base Changing Bulbs Changing the halogen lamp Before you change the lamp it is abso lutely necessary to unplug the power plug from the base to prevent the risk of elec tric shock The halogen lamp becomes very hot during operation Therefore to avoid being burned let the base cool off for approx 10 minutes after switching it off Do not touch new halogen lamps with your bare fingers this drastically redu ces the service life of the lamp Leica Z6 APO A Z16 APO A Changing Bulbs 1 Unscrew the two screws on the heat sink and pull the heat sink out along with the lamp 2 Carefully pull out the lamp and mount by pulling them upwards 3 Disconnect the lamp from the mount 4 Insert the new lamp into the mount and reinsert the lamp holder User Manual Precautionary measures A When inserting the lamp ensure that the cables are inside the two metal clamps This prevents the cab
31. chscreen panel The Leica Application Suite LAS controls and photographs fully automatically as desired User Manual To automate your workflows even more also integrate the Leica IsoPro motorized XY stage it positions multiple specimens fully automa tically or turns a series of individual images into one large seamless illustration to give only two examples Talk to your Leica partner about the wide range of automation possibilities he or she will show you how to get the maximum out of your Leica macroscope With LAS auxiliary module LAS MultiStep With LAS auxiliary module LAS PowerMosaic On We Go If your new Leica macroscope has already been assembled and commissioned by your Leica consultant click here to skip through the instal lation instructions and go directly to the Quick Start Guide on page 45 Leica Z6 APO A Z16 APO A If on the other hand you are assembling the Leica macroscope yourself continue with the Assembly chapter which begins on Page 17 User Manual Assembly Leica Z6 APO A Z16 APO A User Manual 17 Incident light Bases and Manual Focusing Column The first step is to connect the focusing column Assembling the focusing column 2 Hold the incident light to the focusing to the corresponding base 1 Lay the focusing column on the edge of a column and secure it to the base using the table or another stable substrate so that three screws provided
32. d switch briefly Deleting stored positions Press the red switch until you hear the audible signal 2 short beeps pause 2 long beeps 84 Footswitch Focusing 1 Upwards 2 Downwards Leica Z6 APO A Z16 APO A Toggling between coarse and fine focus Press the left and right switches simultane ously Focus positions cannot be stored using the footswitch User Manual 85 Dimensional Drawings Leica Z6 APO A Interface for Tubes 15 Interface for Machine Mounting i 3xM4 6mm depth Leica Z6 APO A Z16 APO A User Manual 87 Leica Z16 APO A 032 9 Interface for Tubes 113 User Manual 88 Leica Z6 APO A Z16 APO A Objectives DIS 41 5 LEICA FEDA 0 8 0446360 x WD 112mm Leica Z6 APO A Z16 APO A LEICA PLANAPO 2 D 10447178 Ox WD 39mm OAS 32 LEICA 1 PLANAPO 0 5 0447177 x WD 187mm User Manual Video phototube AS ab oO _ D58 _ 5 o i LO gt 3 Q Ss oD Interface for Zoom Coaxial Illuminator etc Leica Z6 APO A Z16 APO A User Manual 90 75 5 pe GSC 48
33. een specially designed for the optical requirements of objectives with a high numerical aperture The black rotary knob on the left side of the transmitted light base can be used to rotate the built in path folding mirror and move it forwards back The concave cutout on the handle indicates the concave side of the mirror allowing intuitive operation at any time without eye contact The angle of light incidence in the specimen plane changes depending on the tilt and position of the mirror As a result switching between transmitted light bright field oblique illumination and dark field like illumination is possible User Manual Functions of the rotary knob The rotary knob 1 fulfills the following tasks Turning the deflection mirror from the flat to the concave side Slight tilting to guide the light beam through the specimen plane at a steeper or flatter angle Moving the deflection mirror forwards back 70 Leica TL RCI Color Intensity and Temperature TL SHUTTER 1 Potentiometer for controlling the CCIC Constant Color Intensity Control illumination intensity 2 Potentiometer for controlling the color temperature Leica Z6 APO A Z16 APO A The transmitted light base TL RCI has two electronic potentiometers that control the color intensity 1 and color temperature 2 The controller for the color temperature simul taneously serves as an electronic shutter To interrupt your work click
34. eo phototube A and turn the knurled ring until tight Leica Z6 APO A Z16 APO A User Manual 21 Video Phototube AS Slim The video phototube AS is intended for OEM applications and is usually not used on Leica focusing columns A After installation on the OEM equipment the rest of the components are installed as described on the following pages Leica Z6 APO A Z16 APO A User Manual Video Phototube Y You can use the Y tube to attach binocu 3 Tighten the locking screw on the micro Assembling the tube lar tubes ErgoModules video phototu scope carrier 1 Insert the desired tube and turn the knurled bes and the Leica IC A video module of the Leica ring until tight refer also to page 27 M series Assembling the tube 1 Unscrew the locking screw on the optics carrier 2 Insert the tube into the microscope carrier so that the locking screw faces the rear Leica Z6 APO A Z16 APO A User Manual 23 Coaxial llluminator Optional Tools used Allen key provided The coaxial illuminator is fitted between H the optics carrier and the tube It enables you to connect the light guide which in turn is connected to a cold light source of the Leica CLS series At low magnifications using the coaxial H illuminator causes vignetting shading which is more or less pronounced depending on the configuration This vignetting is not a malfunction A The quarter wave plate for coaxial illumi
35. er Leica Z6 APO A Z16 APO A Separate eyecups are an effective way of preventing infections User Manual 55 Photography amp Video Photography amp Video For most macroscope users digital documen tation has become an invaluable part of their work Research results can be presented in an attractive manner measurements on the digi tal image provide clarity and in conjunction with the motorized IsoPro mechanical stage even images of large specimens can be captu red step by step and automatically joined to create a new complete image Adapters If camera control using the Leica Application Suite is not required conventional mirror reflex and rangefinder cameras from third party manufacturers can be used For this purpose Leica Microsystems offers a variety of adapters that can be used together with the 50 and 100 trinocular tubes Leica Z6 APO A Z16 APO A Leica DFC cameras However if you require absolute control over the camera and need the capability for measu rement evaluation and more in addition to photography the digital Leica DFC cameras are exactly right for you Together with the Leica Application Suite they provide virtually limit less freedom of use For additional informa tion about Leica cameras refer to the camera s documentation User Manual Leica Application Suite The Leica Application Suite or LAS for short is as it were the digital extension of the Leica Z
36. he speci men appears sharp Then open the other eye and correct the diopter settings until the image is uniformly sharp Select the highest magnification and refo cus if necessary User Manual Now if you adjust the magnification from the lowest to the highest level the specimen is always brought into sharp focus If not repeat the process The system is now parfocally matched to your eyes 53 Graticules Leica graticules make length measure ments and counting easier particularly for workstations that are not equipped with a digital camera and LAS software Use The Leica graticules for length measurements and numbering are fitted in mounts and are inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica Z6 APO A Z16 APO A 2 Clamp the graticule on the insert applying 4 You can now align the graticule by rotating moderate pressure Ensure that the grati the eyepiece in the tube and then tighte cule fits tightly ning it using the clamping screw Screw the insert and graticule firmly into place and replace the eyepiece in the tube User Manual 54 Health Notes Potential sources of infection Direct contact with eyepieces is a poten A tial transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepi eces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partn
37. hen manipulating the specimen because the fingers or hand get pinched Accidental contact between the objective and the specimen and potential resulting damage Readjusting the motorized focus The motorized focus is factory adjusted and normally does not need to be readjusted even if the maximum travel path is changed Exception If the power fails while the motorized focus is moving the position data are lost In this case the calibration must be repeated using the Leica LAS software or the Leica SmartTouch To do so please consult the respective manual Leica Z6 APO A Z16 APO A Restricting the bottom travel range 3 Push the limit stop to the height of the 1 Move the motorized focus into the lowest motorized focus position you want to reach Itis easiest to move the limit stop by keeping the Unscrew the screw of the limit stop on the screwdriver inserted and moving it upwards side of the focusing column 4 Tighten the screw of the limit stop User Manual 42 Cables Diagram 1 3 Power plug i CTL2 Ports Z6 216 APO A Powersupply SmartTouch Footswitch 10 450 266 Serial connection loop possible Adapter cable 10447401 needed Serial connection of up to 5 footswitches possible Leica Z6 APO A Z16 APO A Z6 416 APO amp APO A Z Series DCI Box Connection between serial and CTL1 Port User Manual with Z Series DCI Box PC SN A AS TELLE T
38. ined in any way you choose allowing you to create the macroscope that best suits your needs Leica Z6 APO A Z16 APO A Despite this you will notice that the controls and individual components do not differ signi ficantly Whichever configuration you choose you will quickly feel right at home User Manual Have a special request Let us know Leica Microsystems enjoys an exceptional repu tation when it comes to devising customer specific solutions If you have a special request that cannot be met with standard parts contact your Leica consultant He or she has a solution for every problem The Automatic Control System Reliability for your experiments For the Leica Z6 APO A and Z16 APO A auto mated macroscopes motorized control of the zoom iris diaphragm and fine focus is possible This guarantees not only convenient work but also added reliability for your experiments as results can be reproduced exactly In conjunc tion with the Leica LED3000 NVI system illumi The Leica SmartTouch controls all motorized functions ofthe Z series and the connected accessories Leica Z6 APO A Z16 APO A nators a wide variety of scenarios can be set up saved and restored at the touch of a button Control a la carte You alone determine the degree of automation The zoom focus and illumination can be opti onally controlled using the Leica Application Suite LAS software solution or via the Leica SmartTouch tou
39. itted measures x Clean the macroscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety instructions Remove dust with a pneumatic rubber bulb or with a soft brush Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol 112
40. jective 1 Screw the planapochromatic objective to the diaphragm ring or the optional fine focus in a counterclockwise direction User Manual 26 Ergo binocular tube Tools used Assembling No tools required 1 Push the tube into the dovetail ring and 2 While holding the tube only slightly care rotate it slightly in both directions until the fully tighten the positioning screw It is Prerequisite for the installation positioning screw meshes with the guide automatically brought to the correct posi groove tion A When using the ergo binocular tube or another tube the Y video phototube must be used Preparations 1 Unscrew the positioning screw and remove the protective cover Assembling the tube Leica Z6 APO A Z16 APO A User Manual 27 Eyepieces Tools used None Magnification range You can extend the overall magnification H range using available 10x 16x 25x and 40x wide field eyepieces for persons wearing glasses Leica Z6 APO A Z16 APO A Preparation 1 If you want to use an optional graticule insert it now Page 52 2 Remove the plastic tube guard Inserting the eyepieces 3 Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately User Manual 4 Securely tighten the clamping screws 28 Transmitted light Base TL ST Unpacking the base Focusing drive and column The base is delivered with the adapter plate
41. les from getting caught during insertion Leica TL BFDF Transmitted light Base Controls Adapter at the focusing drive Extension plate of the transmitted light Base TL BFDFb 1 Adapter plate for easy assembly of focusing drives 2 Standard stage 10 447 269 3 Button to toggle between bright field and dark field Button to toggle between bright field and dark field Connector for cold light sources light conductor active f 10mm end tube f 13mm Leica Z6 APO A Z16 APO A User Manual 67 Leica TL BFDF Transmitted light Base Operation Light intensity control Observe the user manual in parti A cular all safety regulations from the manufacturers of the light guide and cold light source Switch on the cold light source according to the manufacturer s user manual and adjust the brightness Transmitted light control The Leica TL BFDF transmitted light base has a control that switches the light from bright field to dark field Leica Z6 APO A Z16 APO A Bright field Bright field is suitable for examining translu cent objects featuring contrasting structures The object is directly illuminated from below and is seen in its natural colors against a bright background Turn the control as far as it will go towards BF bright field Dark field In dark field illumination a ring illuminator is used in such a way that the direct light does not reach the objective without a speci
42. light into the eyepieces of the light is available to one or the other beam You can now observe the specimen path The other beam path remains opaque or Turn the controller on the right side of the black tube into the vertical position in order to guide all available light into the camera Assembly You can now photograph the specimen Fasten the trinocular tube 100 to the optics carrier instead of the binocular ErgoTube refer also to page 27 Leica Z6 APO A Z16 APO A User Manual 60 Objectives and Optical Accessories The Different Types of Objectives To meet the various requirements regarding Achromatic objectives with a long focal imaging properties there is a choice of high length quality interchangeable planachromatic and For special applications achromatic objectives planapochromatic objectives and also lower with long working distances and focal lengths priced interchangeable achromatic objectives of f 100 mm to 400 mm are available You can use the objectives on the Z6 APO or Z16 APO zoom systems or with the fine focusing k We recommend using the 1x 2x 5x 0 8x 0 5x planapochromatic objectives in order to take advantage of the high performance of the apochromatic zoom system You can attach the quarter wave plate for coaxial illumination the analyzer for polari zation or a ring illuminator to the planapo chromatic objectives Also available are the M series 0 63x 0 5x 0 32
43. mbly is performed in reverse order of the following steps User Manual Standard stage L Take the glass plate from the rectangular gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base with the four supplied hexagon head screws Insertthe glass plate back into the standard stage 31 TL RC TL RCI The base is delivered with the adapter plate installed The selected stage Leica IsoPro mechanical stage or Leica standard stage 10 447 269 and the focusing drive will have to be mounted later Ensure that the instruments are unpacked on a level adequately sized and nonskid under lay Stage assembly The Leica TL RC RCI transmitted light base can be equipped with three different Leica stages The selected stage is mounted on the base before commissioning You can switch between the stages at any time with just a few hand movements The following paragraph assumes use of the base without the stage mounted Disassembly is performed in reverse order of the following steps Leica Z6 APO A Z16 APO A Standard stage 1 Take the glass plate from the rectangular gap in the standard stage Position the stage on the transmitted light base in such way that the four holes align over those in the base Attach the stage to the base with the four supplied hexagon head screws
44. n materials and manufacture It does not however cover damage resulting from careless or improper handling Leica Z6 APO A Z16 APO A Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Switzerland AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com User Manual Care x Protect your macroscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or repla ced unless doing so is specifically permit ted and described in this manual Protect your macroscope from oil and grease Do not grease guide surfaces or mechanical parts 111 Care Maintenance Contact Persons cont d Protection from dirt Dust and dirt will affect the quality of your results x Put a dust cover over the macroscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces Keep accessories in a dust free place when not in use Leica Z6 APO A Z16 APO A Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuitable cleaning agents and techniques can damage polymers User Manual Perm
45. nal 24 Optics Carrier 25 Objective 26 Ergo binocular tube 27 Eyepieces 28 Transmitted light Base TL ST 29 Leica Z6 APO A Z16 APO A User Manual Transmitted light Base TL BFDF Before First Use 30 Transmitted light Base TL BFDF 31 TL RC TL RCI 32 IsoPro Manual Mechanical Stage Assembly 33 Leica IsoPro Motorized Mechanical Stage Assembly 36 Cables Connections 40 Cables Cable Duct 41 Motorized Focus Adjusting the Travel Path 42 Cables Diagram 43 Leica LED3000 NVI Near Vertical Illumination 46 Quick Start Guide Overview of an automated Leica Z Series Macroscope Leica Z16 APO A 48 Magnification Factors of the Eyepieces 50 Using the Eyepieces 51 The Correct Interpupillary Distance 52 Dioptric Correction 53 Graticules 54 Health Notes 55 9 Photography amp Video Photography amp Video 57 Photo Tubes and C mounts 58 Trinocular Video Phototube 50 59 Trinocular Video Phototube 100 60 Optical Accessories The Different Types of Objectives 62 Leica TL ST Transmitted light Base Controls 64 Leica TL ST Transmitted light Base Operation 65 Leica TL ST Transmitted light Base Changing Bulbs 66 Leica TL BFDF Transmitted light Base Controls 67 Leica TL BFDF Transmitted light Base Operation 68 Leica TL RC TL RCI Controls 69 Leica TL RCI The Deflection Mirror 70 Leica TL RCI Colo
46. nce 3 Slowly approach the eyepieces with your see a single circular image field when looking 1 Look into the eyepieces eyes until you can see the complete image at a specimen 2 Hold the eyepieces with both hands Push field without corner cutting the eyepieces together or separate them until you see a circular image If you are still a novice macroscope user you may need a short time to become accustomed to this Not to worry after a little while it will become automatic Reference value The distance between eye and eyepiece measu res approx 22 mm for 10 23B wide field eyepi eces for persons wearing glasses Leica Z6 APO A Z16 APO A User Manual 52 Dioptric Correction Leica macroscopes are parfocally matched A prerequisite for this the correct setting of the diopters The following adjustments only have to be carried out once by each user Therefore all Leica eyepieces are also available with built in dioptric correction allowing the macroscope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica Z6 APO A Z16 APO A Using the Dioptric Correction 1 Set the dioptric correction of both eyepi eces to the mid position 0 diopter settings Look through the eyepieces and focus on a flat specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in the direction until t
47. ng personal eyepieces for each individual or detachable eyecups User Manual Safety Instructions cont d Light sources Safety Instructions Protective measures of the manufacturer x UV protection screen in front of the speci men plane prevents the user from looking directly into the UV rays Dummy filter carriers in the free positions of the rapid filter changer prevent direct UV radiation from reaching the eyes UV filters are installed in the observation beam paths to protect the eyes The stray light protection on the lamp housing prevents irradiation of the hands Leica Z6 APO A Z16 APO A Warning UV radiation could damage the eyes Therefore Never look into the light spot on the speci men plane without a UV protection screen Never look into the eyepieces if no excita tion filter is in the beam path Fill empty filter positions with dummy filter carriers Do not select a white strongly reflective background for the specimen Supply unit Always unplug the supply unit from the power sup ply When installing and disassembling the lamp housing Before opening the lamp housing When replacing the high pressure mercury lamp and other parts such as the heat absorbing filter or the collector During maintenance work on the supply unit User Manual Lamp housing ra Never open the lamp housing while the lamp is switched on Risk of explosion UV exposure blinding Before openi
48. ng the lamp housing allow it to cool off for at least 15 minutes Danger of explosion Never cover the air duct on the lamp housing Danger of fire Safety Instructions cont d Mercury lamp Follow the user manual and safety instruc tions provided by the lamp manufactu rer and in particular the section on how to proceed if a lamp breaks and releases mercury For transport remove the mercury lamp transport it in its original packaging and protect moving parts in the lamp housing using the transport anchors When it has reached the end of its rated life follow the manufacturer s specifications and the minute meter on the supply unit To minimize the risk of explosion replace a discolored mercury lamp promptly Leica Microsystems assumes no liability for damage caused by exploding incor rectly installed or improperly used mercury lamps Leica Z6 APO A Z16 APO A User Manual 8 Table of Contents General Instructions 2 Important Safety Notes 3 Symbols Used 4 5 Safety Instructions The Leica Z Series Congratulations 13 The Modular Design Everything is Relative 14 The Automatic Control System 15 On We Go 16 Incident light Bases and Manual Focusing Column 18 Transmitted light Bases and Manual Focusing Column 19 Microscope Carrier 20 Video Phototube A 21 Video Phototube AS Slim 22 Video Phototube Y 23 Coaxial Illuminator Optio
49. parent and transparent 1 Push the deflection mirror all the way back specimens The phase structures look like hills specimens These settings result in a nega towards the column tive relief contrast Phase structures look like The effect can be strengthened or weakened by valleys 2 Turn the deflection mirror into the notch gently tilting the deflection mirror position at an angle of 45 The effect can be strengthened or weakened by gently tilting the deflection mirror Leica Z6 APO A Z16 APO A User Manual 74 Leica TL RCI Relief Images cont d Dynamic relief contrast Suitable for semitransparent and transparent specimens By tilting the deflection mirror slightly you can move the gaps over the entire field of view and quickly toggle between positive and negative relief images The dynamic effect makes it easy to distinguish phase structures from amplitude structures Leica Z6 APO A Z16 APO A Constraints The relief methods provide good results from mid zoom to high magnifications and with 1x 1 6x and 2x objectives In the lower zoom half and with weaker specimens the object field may not be uniformly illuminated We recommend using the transmitted light base with 1x or higher objectives and not objectives with a long focal length User Manual Using Filters Filters for Leica TL RC and TL RCI The transmitted light bases TL RC and TL RCI can be equipped with up to three filte
50. r Intensity and Temperature 71 Leica TL RC TL RCI Operation 72 Leica TL RCI Methods in Transmitted Light 73 Leica TL RCI Relief Images 74 Using Filters 76 Leica IsoPro Non motorized Controls 77 Leica IsoPro Motorized Controls 78 Leica Z6 APO A Z16 APO A System Illumination Leica LED3000 NVI Near Vertical Illumination 80 Leica PSC Controller 82 Leica SmartTouch 83 Manual Control 84 Footswitch 85 Leica Z6 APO A 87 Leica Z16 APO A 88 Objectives 89 Video phototube AS 90 Y tube 91 Video Phototube A 92 Microscope carrier 93 Focusing arm for probers 94 Focusing arms 95 Adapters 96 Coaxial incident light housing 97 User Manual 10 Leica Z6 APO A amp Z16 APO A 99 Optical Accessories for Leica Z6 APO A amp Z16 APO A 100 Ambient Conditions and Weights 101 Optical Data Visual with Y Tube 1 25x 102 Leica TL ST Transmitted light Base 105 Leica TL BFDF Transmitted light Base 106 Leica TL RC TL RCI 107 Leica IsoPro Motorized XY Stage 108 Appendix Calculating the Total Magnification and Field of View Diameter 110 Care Maintenance Contact Persons 111 Leica Z6 APO A Z16 APO A User Manual 11 Leica Z6 APO A Z16 APO A User Manual Congratulations Congratulations on obtaining your new Leica Z series macroscope We are convinced it will exceed your expectations as the Z series embodies all the qu
51. r supply Input 100 240 VAC 50 60 Hz 1 0 A Output 15 VDC 2 66 A 40W max Leica Z6 APOA Z16APOA User Manual 108 Appendix Leica Z6 APO A Z16 APO A User Manual 109 Calculating the Total Magnification and Field of View Diameter Parameter MO Magnification of the objective ME Magnification of the eyepiece Example MO 1x objective ME 25x 9 5 eyepiece Z Magnification of the changer position q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 ErgoTube r Factor of 1 25x when using video phototube Y Z Zoom position 4 q Coaxial incident light 1 5x tube factor r Factor 1 25x NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Calculation example Magnification in the binocular tube MTOT VIS MOxMExzxqxr or 1x25x4x 1 5 x 1 25 187 5x Leica Z6 APO A Z16 APO A Calculation example Field of view diameter in the specimen NFOV Moxzxqxr User Manual 110 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance macroscope Leica macroscopes are renowned for their robustness and long service life Observing the following care and cleaning tips will ensure that even after years and deca des your Leica macroscope will continue to work as well as it did on the very first day Warranty benefits The warranty covers all faults i
52. rs available as accessories simultaneously By customer request the filters are also available as one off items 1 Switch off the light source or click Leica TL RCI the button for the shutter t SL SHUTTER Leica Z6 APO A Z16 APO A 2 Take the empty filter from an available filter slot in the filter holder 3 Insert the desired filter 4 Switch the light source back on User Manual Daylight filter for Leica TL ST A daylight filter is also available for the Leica TL ST transmitted light base 76 Leica lsoPro Non motorized Controls Operating the Leica IsoPro mechanical stage 1 To move the stage in the X direction rotate the outer knob 2 To move the stage in the Y direction rotate the inner control ring Leica Z6 APO A Z16 APO A User Manual 77 Leica lsoPro Motorized Controls IsoPro X Y Stage DCI module Leica PSC Controller 1 Sub D interface for Leica SmartMove 1 Quick control memory function 2 3CTL2 interfaces 2 Fine control in X direction n i 3 USBinterface type B 3 FinecontrolinYdirection 1 LeicalsoPro motorized mechanical stage 4 Socket for grounded power cable 2 Housing with motorization Leica Z6 APO A Z16 APO A User Manual 78 System Illumination Leica LED3000 NVI Near Vertical Illumination H The intensity of the illuminator can be adjusted in 10 increments A The Leica LED3000 NVI can also be controlled via the Leic
53. ructions cont d Transport Use the original packaging for shipping or transporting the individual modules and the accessory components In order to prevent damage from vibra tions all moving parts that according to the user manual can be assembled and disassembled by the customer should be disassembled and packed separately Integration in third party products x Refer to Safety Concept booklet Disposal Refer to Safety Concept booklet Legal requirements Refer to Safety Concept booklet EC Declaration of Conformity Refer to Safety Concept booklet Leica Z6 APO A Z16 APO A Health risks Workplaces equipped with macroscopes faci litate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arran gement of workplace work assignments and work flow changing tasks frequently Thorough training of the personnel giving consideration to ergonomic and organizati onal aspects The ergonomic design and construction of the Leica Z series are intended to reduce the exer tion of the user to a minimum Direct contact with eyepieces can be a poten tial transmission path for bacterial and viral infections of the eye The risk can be kept to a minimum by usi
54. stage and turn it around Attach the axis with the control buttons to the desired side The fastener snaps into the mechanical stage magnetically Attach the axis with the two supplied hexa gon head screws Attach the cover rail to the mechanical stage User Manual Mechanical stage assembly 1 2 Place the mechanical stage onto the base Pull the upper part of the mechanical stage carefully towards the user fixing the lower part onto the transmitted light base Attach the mechanical stage evenly to the three threaded holes Now move the mechanical stage as far as it will go in the direction of the column Insert the glass plate into the mechanical stage 34 Leica IsoPro Manual Mechanical Stage Assembly cont d Focusing drive and column 1 Unscrew the extension plate from the base using the Allen key provided 2 Attach your focusing drive column from below using the three hexagon head screws 3 Re attach the adapter plate to its original position using the three hexagon head Screws Leica Z6 APO A Z16 APO A User Manual 35 Leica IsoPro Motorized Mechanical Stage Assembly Basics 1 Unpack the mechanical stage from the 2 Secure the motorized mechanical stage to Thetransmitted light bases of the Leica TL series transport packaging and position it on the the base using three M4 screws TL BFDF TL RC TL RCI are supplied with an transmitted light base installed extension plate
55. ubes ergonomics e Inclined and straight binocular tubes e Apochromatic ErgoTube 10 50 with synchronized interpupillary distance adjustment e Various ErgoModules optional Interpupillary distance 55 mm 75 mm Ergonomic wide field eyepieces 10x 21 16x 14 25x 9 5 40x 6 distortion free attachable eyecups for protection from infection for persons wearing glasses Electrical interface RS232 USB via motorized focus ErgoTube and ErgoModule are registered in the United States Patent and Trademark Office Leica Z6 APO A Z16 APO A User Manual 100 Ambient Conditions and Weights Ambient operating conditions Weight Ambient temperature 10 C to 40 C 10447 174 Leica Z6 APO A zoom system 1 030 kg Rel humidity Up to 35 C ambient temperature 75 10 447173 Leica Z16 APO A zoom system 1 330 kg Air pressure 700 1060 hPa 10 447 176 1x planapochromatic objective 0 150 kg Transport and storage 10 447 178 2x planapochromatic objective 0 270 kg Temperature 20 C to 52 C 10 446 360 0 8x planapochromatic objective 0 170 kg Rel humidity 10 95 non condensing 10 447177 0 5x planapochromatic objective 0 170 kg Air pressure 500 1200 hPa 10 447 204 Video phototube AS 0 120 kg 10 447 128 Video phototube A 0 200 kg 10 447 109 Y tube 0 430 kg 10 447 196 Carrier for AS tube 0 340 kg Leica Z6 APO A Z16 APO A User Manual 101
56. width 4 2 0 7um4 9 0 74um Numerical Aperture 0 04 0 234 nA 0 034 0 224 nA Object field 4 2 mm 0 67 mm 4 2 mm 0 26 mm Data with Leica DFC490 digital camera 1x planapochromatic objective AS tube 0 63x video objective Magnification Chip Object 0 36x 2 3x 0 36x 5 8x Digital resolution 33 3 210 Lp mm 33 3 336 Lp mm Object field projected onto chip Depth of field diaphragm open 24 5 mm x 18 4 mm 3 9 mm x 2 9 mm 1 06 mm 0 03 mm 10 7 mm 0 26 mm Depth of field dia closed Leica Z6 APO A Z16 APO A 24 5 mm x 18 4 mm 1 5 mm x 1 14 mm 1 4 mm 0 03 mm 10 9 mm 0 3 mm User Manual 99 Optical Accessories for Leica Z6 APO A amp Z16 APO A Leica Z6 APO A amp Z16 APO A Objectives e Planapochromat 1x 2x 0 8x 0 5x 5x e M series achromatic objectives 0 63x 0 5x 0 32x lead free 0 5x 0 32x lead free Working distances e 187 mm 0 5x planapochromat 97 mm 1x planapochromat e 112 mm 0 8x planapochromat 39 mm 2x planapochromat 19 mm 5 0x planapochromat Objective adapters For M series achromatic objectives e For 10x and 20x HR objectives e For 10x and 20x DM objectives DM objective N Plan L 20x 0 40 corr DM objectives suitable for the upper zoom range only e DM objective N Plan 10x 0 25 A5 8 Fine Focusing 10 mm path motorized and integrated Binocular t
57. x achromatic objectives which can be attached to the zoom or the fine focusing using an adapter You can attach the vertical illuminator or the attachment for incident oblique observation to the achromatic objective Leica Z6 APO A Z16 APO A User Manual 62 Leica Z6 APO A Z16 APO A User Manual 63 Leica TL ST Transmitted light Base Controls Extension plate of the transmitted light base TL ST Rear side of the transmitted light base TL ST 1 Screws for changing the halogen lamp 2 Power connection socket 1 Adapter plate for easy assembly of focusing 3 Power switch drives 2 Removable glass plate Controller for light intensity 4 Adjustment for deflection mirror Uy Leica Z6 APO A Z16 APO A User Manual 64 Leica TL ST Transmitted light Base Operation Light intensity control The left control adjusts the intensity of the 12V 20W halogen illumination 1 Switch on the illumination of the base at the power switch 2 Focus on the specimen 3 Set the illumination to the desired intensity using the left control Leica Z6 APO A Z16 APO A Transmitted light control The transmitted light base TL BFDF has a slider that automatically moves the deflection mirror in the base when moved The mirror is kept in the correct position at all times and permits smooth changeover between bright field and opaque transmitted light User Manual Bright field Bright field is suitable for examining translu

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