ÄKTA avant Operating Instructions
Contents
1. 54 AKTA qvant AKTA qvant 29 1015 56 AA 4 4 1 4 1 2 AKTA avant 66 cm AKTA avant 9 kg 3 kg 130 kg AKTA qvont 29 1015 56 AA 55 4 4 12. AKTA avant 25 AKTA avant 150 AKTA avant 25 AKTA avant 150 0 001 25 ml 0 01 150 ml Column packing 7ow Columnpacking 7ow 50 m min 300 7m 20 MPa 200 5 MPa 50 190 700 nm 190 700 nm AKTA avant 29 1015 56 AA 33 3 3 1 AKTA avant 2 3 4
3. 2011 65 EU RoHS 2012 19 EU WEEE ACPEIP RoHS EC REACH 1907 2006 10 AKTA qvant 29 1015 56 AA 1 1 3 AKTAavant EN IEC 61010 1 CE AKTA qvont 29 1015 56 AA 11
4. gt AKTA qvont 29 1015 56 AA 1 1 3 1 3 AKTA qvant
5. 2 AKTA qavant AKTA qvant 25 2 10 AKTA aqvant 150 40 30 c m
6. Superloop Superloop Syr Superloop Sysrem CJP Column Cp 20 AKTA avant 29 1015 56 AA
7. P9 P9H A P9H B P9HS O 2 150 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H 0 PumpP9 T20 Pump P9H T10 T20 20 PumpP9 6 8 MPq 60 80
8. 1 W2 Sysrem Contro AKTA qvont 29 1015 56 AA 91 4 4 5 4 5 2 Sqample Pump 4 Process Pjcfure Sampleinlet vg ve
9. 178 2M 70 1M 1M Column Valve V9 C Column Valve V9 C 1M BufferPro Quaternary Valve Q9 0 2 M HCI
10. 50 ml 20 76 AKTA qvant 29 1015 56 AA 4 42 4 2 6 4 2 6 1 Power 1 Notconnected 2 AKTA qvont
11. WT AKTA qvant 29 1015 56 AA 6 6 2 Injection Valve Syr Empy oopwi Sample Application Pose Properties Phase Properties Text Instructions iT Sample Applicaton Use the same Flow rate as in Method Settings Flow rate 10 000 mlzmin OD OOD 25 000
12. 4 1 1 50 4 1 2 52 4 1 3 56 4 1 4 57 4 1 5 59 4 1 6 61 AKTA qvont 29 1015 56 AA 49 4 4 1 4 1 1 4 1 1 N AKTA avqnt 116 kg 4 GE
13. P9 S 0O 2 AKTA qvont 29 1015 56 AA 161 7 7 3 Pump P9 5 0 162 T20 20 Ref 1 P9 S Seql kit 65 ml Pump P9 S
14. 2 4K7A avant User Manual 1 Injection Valve LoopF LoopE TO 77 AKTA avant 29 1015 56 AA 131 132 6 G62
15. System flow 1 000 mi min 0 0 ssB Manual load PreC 0 23 MPa Cond 0 05 ms cm pH Off 0 50 Uvi 593 845 maUu Temp 20 5 C aia E P mm VM EE SyP F 1 UV EN Cond mm Restrictor mm Frac mE A High rm Available components without a fixed place 43 839 mal I O box AKTA qvont 29 1015 56 AA 141 6 6 4 Process Picture Process Pjcture njecron valve
16. AKTA avant AKTAavant25 AKTA avant 150 34 AKTA avant 29 1015 56 AA 15 13 12 Injection Valve 3 3 1 AKTA avqnt 11 10 Flow Restrictor pH Valve Column Valve UV Monitor Conductivity Monitor Inlet Valve B Quaternary Valve system Pump B Inlet Valve A system Pump A Mixer Sqmple Pump Sample Inlet Valve Outlet Valve AKTA avant 29 1015 56 AA
17. AKTA qvant 116 kg 4 3 GE 18 AKTA avant 29 1015 56 AA 2 2 1
18. AK7A avant User Mgnug AKTA qvant 29 1015 56 AA 109 5 5 3 5 3 4 5 82 110 AKTA qvant 29 1015 56 AA 5 5 4 54
19. s T20 AKTA avant 29 1015 56 AA 7 7 4 PumpP9 P9H Z2 Pump P9 Pump P9H 0
20. AKTA avant 29 1015 56 AA 123 5 5 7 124 50 ml 250 ml 1 2 50 ml 250 ml GE 2 50m 250 ml lt AKTA qvant 29
21. RPC F9 R AKTA qvant 29 1015 56 AA 2 2 1 RPC 100 5 MPa 50 AKTA qvqnt 25 PEEK 100 RPC 0 5 mm PEEK 10 MPa 100 RPC AKTA avant 150 100 100 RPC
22. a T 140 AKTA qvant 29 1015 56 AA 6 6 4 UNICORN UMCORASysfem Control Mgnuog Process Picture process Pcfure
23. AKTA ovont Microsoft Windows e UNICORN Administration and Technical Mognuo AKTA qvant 29 1015 56 AA 1 1 2 A
24. 3 UP 1 2 AKTA avant 29 1015 56 AA 167 7 7 3 Pump P9 5 0O
25. 2 AKTA avant 29 1015 56 AA 29 2 2 3 3 Continue Sysre Control Continue 30 AKTA qvant 29 1015 56 AA 2 2 4 24 AKTA avant AKTA avant
26. RPC F9 R GE AKTA qvont 29 1015 56 AA 25 2 Ke 5 SJ 771363 2006
27. GE UMCORMAgm njstro0o7 and Technical Mgnuo AKTA qvont 29 1015 56 AA 69 4 4 2 4 2 3 4 2 3 JJ MD 0 GB OM Gh ss ss 2 2 GBD mb GD GBD Gb ED ED GD GBD pH W W1 W2 W3 70 AKTA qvant 29 1015 56 AA 4 42 4 2 3
28. PPE AKTA avant 29 1015 56 AA 17 2 2 1 N AKTA qvant AKTAqvant
29. RPC F9 R RPC 100 5 MPa 50 AKTAavant 25 PEEK 100 RPC 0 5 mm PEEK 10 MPa 100 RPC AKTA avant 150 100 100 RPC PEEK
30. AKTA avant 29 1015 56 AA 61 4 4 1 4 1 6 mm NUNC NUNC SARSTEDT Thermo Scientific BDTM Biosciences VWRTM BD BioscienceS BD BioscienceS 24 48 96 10 5 2ml 62 AKTA qvant 29 1015 56 AA
31. t_i 7 3 4 5 10 ml 1ml 94 AKTA qvant 29 1015 56 AA 4 4 5 4 5 2 Sqample Pump 8 _ 9 6 8 10 System Pump A BB Sample Pump 90 System Control End AKTA qvont 29 1015 56 AA 95 4 4
32. 26 AKTA qvant NTRL NRTL 29 29 CFR Part 1910 7 OSHAJ AKTA qvant 29 1015 56 AA 2 2 3 23 AKTA qvant pguse
33. ID ID D ID System Pump A ID P9A P9HA ID system Pump B P9B P9HB Sqmple Pumbp P9 S P9HS Pressure Monitor R9 Pressure Monitor R9 Mixer M9 Injection Valve v9 Inj V9H Inj Quaternary Valve Q9 Inlet Valve A V9 IA V9H IA Inlet Valve B V9 IB V9H 1 IB Sample Inlet Valve V9 IS V9H 2 IS Column Valve V9 C 5 V9H C AKTA avant 29 1015 56 AA
34. 4 5 1 83 4 5 2 Sqmple Pump 91 4 5 3 O 96 82 AKTA qvant 29 1015 56 AA 4 4 5 4 5 1 4 5 1 1 2 3 System Pump B System Pump B System Pump A System Pump A
35. 2 IEC60950 AKTA qvont 29 1015 56 AA 65 4 4 2 4 2 2 4 2 2 AKTA avant AKTA avant GE 1 Power 2 N
36. Outlet Valve V9 O V9H O 10 UV U9 M 190 700 nm 3 UV Vis C9 oO Built in fraction collector AKTA qvont 29 1015 56 AA 39 3 3 1 AKTA qvant 40 2 In
37. Pump P9 S i 168 20 S1 4 5 2 Sample Pump 917 Ref 1 2 3 MPq 20 30 1A 18 AKTA qvant 29 1015 56 AA 7 7 3 Pump P9 5 0O 5 5 Manualiinstructions Flow path Column position
38. AKTA avant I N 5 5 176 AKTA qvont
39. 1 pH 4 W W1 W2 W3 2 30 c m 3 2
40. AK7A avant 25 Product Documentation AKTA avant 150 Proguct Documentation 8 1 176 8 2 178 8 3 D 183 AKTA qvont 29 1015 56 AA 175 8 8 1 8 1 UNICORNT 6 0 PC x x 860x710x660 mm 116 kg 100 C 240 C VAC 50 C 60 C Hz 800 VA IP 21 IP 22 FEP id 1 6mm 5 16 1 8 PEEK 0 75 m
41. 100 MSDS MSDS CAS no EC no N A pH 2 12 CIP 2 CAS no EC no 70 75 05 8 200 835 2 AKTA avant 29 1015 56 AA 179 8 8 2 CAS no EC no Decon 90 N A 100 75 08 1 200 837 3 100 67 56 1 200 659 6 0 1 M 7647 01 0 231 595 7 100 67 63 0 200 661 7 2 M 1310 73 2
42. AKTA avont 20 cm
43. AKTA qvont 29 1015 56 AA 105 5 5 2 Pressure Monitor Sqmple Pumbp Sqmple Inlet Valve Quaternary Valve Inlet Valve A Inlet Valve B SVSterm Pump A 106 AKTA qvant 29 1015 56 AA 5 5 2 system Pump B Pressure Monitor Mixer Injection Valve Superloop Column Valve UV Monitor Conductivity Monitor pH pH valve Flow Restrictor Outlet Valve AKTA qvont 29 1015 56 AA 107 5 5 2
44. 20 C Sysre Control System Settings Method Editor Text Instructions 126 AKTA qvant 29 1015 56 AA 6 6 6 1 128 6 2 131 6 3 134 6 4 140 6 5 143 AKTA qvont 29 1015 56 AA 127 128 6 6 1 6 1
45. Hold i Pause Il Continue AKTA qvont 29 1015 56 AA 129 6 6 1 Eng 130 AKTA qvant 29 1015 56 AA 6 6 2 6 2
46. AKTA avant 29 1015 56 AA 59 4 4 1 4 1 5 60 1280x1024 UMICORVM Windows UMCORVM Windows Windows 7 Aero Windows UNICORN Windows 7 gn DPI 100 UNICORN
47. GE 100 240 VAC 50 60 Hz I 800 VA 1 3
48. AKTA avant mm AKTA qvont 1000 x 900 x 800 155kg x x 25 C 60 C 20 95 50 AKTA qvant 29 1015 56 AA 4 4 1 4 1 1 80 x 100 cm AKTA avant AKTA qvont 4K7A avant Unpacking Instructions AKTA
49. Process Picture Y Mgnuo jnstruchons A B 1 Sysrem Contro AKTA qvont 29 1015 56 AA 83 4 4 5 4 5 1 3 84 process Pcrure
50. AKTA avant UniNet 9 UniNet 9 Firewire AKTA avant UniNet 9 AK7A avant User Mgnuo UniNet 9 AKTA avant 29 1015 56 AA 19 2 2 1 A AKTAqvant
51. AKTA qvont 29 1015 56 AA 2m 57 4 4 1 4 1 4 AKTA avant 58 AKTA qvant 29 1015 56 AA 4 4 1 41 5 4 1 5 AKTA avqgnt PC UNICORN PC PC AKTA UNICORN
52. 5o g ro jc Flowpath Injection valve Flowpath Injection valve bold Power AKTA qvont 29 1015 56 AA 1 1 2 1 2 IR AKTA qvant
53. UNICORN Yes No Cancel Power OO AKTA avant 29 1015 56 AA 145 AKTA qvant AK7A avant User Monug N PPE
54. EU DoC GE Healthcare Bio Sciences AB BIOrkgatan 30 SE 751 84 Uppsala Sweden EU EU 2006 42 EC MD 2004 108 EC EMC 2006 95 EC LVD 1999 5 EC R amp TTE 8 AKTA qvant 29 1015 56 AA CE CE 1 1 3 CE EU GE
55. 4 es 1 4 5 T20 2 UN MT QQ AKTA qvont 29 1015 56 AA 153 7 7 2 Pump P9 Pump P9H 0 154 PumpP9 1T20 2 Pump P9H T10 4 0O 1 2 P9 P9H 2 0 T20
56. AKTA avant 29 1015 56 AA 4 4 5 4 5 1 8 System Pump A 8 Sompje Pump 90 System Pump B 85 System Pump B System Pump B System Pump B 1 W1
57. EN ISO 12100 EN ISO EU 2006 42 EC EN IEC 61010 1 UL 61010 1 CAN CSA C22 2 No 61010 1 EN EU 2006 95 EC EN IEC 61326 1 CISPR 11 1 A EMC EN EU 2004 108 EC ETSI EN 301 489 3 ERM EMC EN EU 1999 5 EC AKTA avant 29 1015 56 AA 1 1 3
58. PEEK PEEK 0 75 mm AKTA qvant PPE AKTA qavant
59. 1 5 20 6 8 NE 158 Pump P9 P9H 1 20 A1 System Pump A B1 System Pump B S1 Sample Pump 2 4 5 1 83 AKTA qvant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H 0
60. Ns Injechon valve Manual load SYSterm pump waste Inject Sample pump load Direct inject Sampls pump waste Detailed picture 142 AKTA avant 29 1015 56 AA 6 5 6 6 5 2 3 pH 7
61. AKTA qvont 29 1015 56 AA 23 2 AG 2 2 AKTA qvqnt AKTA avant AKTA avant 24 AKTA qvant 29 1015 56 AA 2 22 28930842 AKTA qvant 25 Code no 28924630 Voltage 100 240 V Serial no Freduency 50 60 Hz Mfg Year Max Power 800 VA Protection Class IP21 Wet Side IP22 a N CE HDR LN GE Healthcare Bio Sciences AB Made in Sweden 751 84 Uppsala Sweden 86 Conforms to ANSI UL Std 61010 1 Cert to CAN CSA Std C22 2 No 61010 1
62. AKTA qvant AKTA qvont AKTA avqnt Ethernet UV UV UV 2 MPa 20 5 MPa 50 l AKTAqvant 22 AKTA qvant 29 1015 56 AA 2
63. UNICORN Sysrem Seftings Sysre Sejngs UMCORASyse Control Manual Sysre Settings AKTA avant User Monug 120 AKTA qvant 29 1015 56 AA 5 5 7 1 QuickReleose 2 A1 A1 AKTA avant
64. 5 1 103 5 2 105 5 3 110 5 4 111 5 5 116 5 6 pH 118 5 7 120 5 8 126 102 AKTA avant 29 1015 56 AA 5 5 1 5 1 Method Edjror A AKTAaqvant
65. RS ed 4 1 AKTA qvont 29 1015 56 AA 31 3 AKTA qavant lt 3 1 AKTA qvant 33 3 2 UNICORN 43 UNICORN AKTA avant I 32 AKTA avant 29 1015 56 AA 3 3 1 AKTA qvqnt 3 1 AKTA gvant AKTAavant AK7A ogvgn User Monug AKTA qvant
66. 10 40 kg AKTA qvont 29 1015 56 AA 21 2 2 1 2 AKTA avant UV Superloop Manualinstructions Superloop AKTA avant
67. PPE GE AKTA avant 29 1015 56 AA pH RPQI 103 5 5 1 104 AN AKTAaqvant
68. 2 AKTA avant 29 1015 56 AA 173 7 7 5 10 11 90 174 AKTA qvant 29 1015 56 AA 8 AKTA avant
69. AKTA qvant 29 1015 56 AA 139 6 6 4 6 4 System Conro System stgfe Aun Doto Run Wash Hold Chromatogram Run Log Flow Scheme System Conro 3 2 2 46 Sysre Control Hold Pguse Eng Conjnue
70. UNICORN Sysre Control pguse SS System Cnntrnl File Edt Wiew Manual Swstem Tools Help y EL AKTA qvont 29 1015 56 AA 27 2 2 3 0 power O UNICORN 28 AKTA qvant 29 1015 56 AA 2 2 3 UNICORN
71. 100 1 UMICORM AKTA qvant 29 1015 56 AA 4 1 6 4 4 1 4 1 6 AKTA avant 1 Quaternary Volve 1 0 5 Ouoternary Valve 1 1M 0 5 20
72. Position sert Flow path Sample inlet Position nserr Flow path Injection valve Position Directinject serr Pumps Sample flow Flow rate 25 0 m Insert Click Execute 25 0 ml 6 2 7 AKTA qvont 29 1015 56 AA 169 7 74 7 4 170 P9 P9H P9 S 0 2
73. GE 2 96 GE 4 4 1 4 1 6 7701 5200 BD Biosciences 353966 Greiner Bio One 780270 Porvalir Sciences 219009 Seahorse Bioscience Eppendorf S30009 951033405 0030 501 306 48 GE 7701 5500 Seahorse Bioscience S30004 24 AKTA qvont 29 1015 56 AA GE 701 5102Z Seahorse Bioscience S300Z4 63 4 4 2 4 2 AKTA qvant AKA avant AKTA avant Unpacking Instructions I A
74. AKTA qvant 29 1015 56 AA 7 7 3 Pump P9 5 O 3 20 157 1 2 2 20
75. GE 2015 General Electric Company All rights reserved 2015 3 GEHeqlthcare GE Healthcqre GE Heqlthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germqny GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Heqlthcare Bio Sciences COrD 800 Centennial Avenue PO Box 1327 Piscatawoy NJ 08855 1327 USA GE Healthcqre Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 1015 56 AA 03 2015 q24
76. I AKTA qvont pH UV pH AKTA avant 29 1015 56 AA 147 148 AKTA avqnt AK7A gvgn User Manual System CIP Column CIP pH pH
77. 7 1 147 7 2 Pump P9 Pump P9H O 150 7 3 Pump P9 S 0 161 7 4 170 7 5 172 146 AKTA avant 29 1015 56 AA 7 A 7 1 AKTAovont AK7A avant User Manual N GE
78. 9 System Pump A 8B Sompje Pump 90 Sgmpje Pump 93 Sample Pump 1 2 W2 3 Syste Conro 4 process Picture
79. P9 P9H 4 0O 21 4 x 1 6 mm 20 O P9 P9H AKTA avant 29 1015 56 AA 157 7 7 2 Pump P9 Pump P9H 0 151 1 2 20 3 4 T20 2 1
80. 3 PumpP9 Ref2 6 8MPa 60 80 1A 18 PumpP9H Ref 1 2 3 MPa 20 30 1A 1B 4 AKTA qvant 29 1015 56 AA 159 7 7 2 Pump P9 Pump P9H 0 Manualiinstructions Flow path Column vo ve sert Flow path Inlet A System Pump A
81. 150 7 2Pump P9 PumpP9 0 150 Pump P9 S Pump P9 S 7 3 Pump P9 5 0 1617 AKTA qvont 29 1015 56 AA 171 7 7 5 7 5
82. Open Method Navigator Method Navigator Run Hold pguse Continue Eng AKTA qvont 29 1015 56 AA Customize Customize Connect to Systems Connect to Systems 47 4 48
83. 4 5 3 O 4 3 4 10 ml Q1 5 6 Quaternary start concentration 100 2 5 Q2 Q3 Q4 BufferPro 07 02 7 System Pump A B Sample Pump 90 Quaternary valve 98
84. AN PPE PPE
85. Windows 7 Professional 32 64 Service Pack 1 NTIete mn 5 E License N 3 GB 3 GB 3 GB 2 GB RAM NTFSINT NTFSINT NTFSINT NTFSINT OS Windows 7 Windows 7 Windows 7 Windows 7 Professional SP1 Professional SP1 Professional SP1 Professional SP1 32 64 32 64 32 64 32 64 Windows Server Windows Server 2008 R2 64 2008 R2 64 1033 1033 1033 1033 Intel Intel Intel Intel UNICORN
86. pH gy pgss pH pH pH AK7A avant User Manual 144 AKTA avant 29 1015 56 AA 6 6 5 UNICORN UNICORN UNICORN UNICORN UNICORN Fe L og o Log off Eric Ei LMILGRTM UNICORN Log 0n UNICORN Fe Exit UNICORN Lnn oFF Eric Exit UNICORN UNICORN
87. 1 J le inlet valve Buffer s1 sz sa s4 op op sz 5 25 30 ml 6 3 4 7 92 AKTA qvant 29 1015 56 AA 4 4 5 4 5 2 Sqample Pump 8 2 5
88. System Pump A Inlet Valve B V9 IB V9H IB 7 System Pump B Sample Inlet Valve V9 IS V9H IS 8 7 1 AKTA qvant 29 1015 56 AA Injection Valve V9 Inj V9H Inj Column Valve V9 C V9H C 3 3 1 AKTA avant 5 1 2 pH Valve V9 pH V9H pH pH pH pH Valve
89. 2M 10 AKTA qvant 29 1015 56 AA 8 8 2 100 0 2 TFA 5 100 PEEK
90. sSysrem Settings UMCORA Method Manual Sysrem Conrro 1 Manual System Conro Execure Manual Instructions Mgnug instructions Instructions Alarms Alarm pre column pressure Selected column type Parameters for amp larm pre column pressure Mode O Disabled Enabled High alam IQ02 200m 0 50 MPa Low alam 0 00 20 00 0 30 MPa 3 Mode Enabled
91. AKTA avant AK7A avant AKTA avant Unpacking Instructions 4 1 4 2 4 3 4 4 UNICORN 4 5 4 6 49 64 78 79 82 101 AKTA avant 29 1015 56 AA 4 4 1 41 AKTAqvont AKTA ovunt
92. 100 1 100 2 3 172 AKTA avant 29 1015 56 AA NL dl 7 7 5 gt DS KO I a lt 4 4 eS So gt 0
93. Injection Valve pH Volve Outlet Valve InjectionVaqlve V9 Inj v9H Inj e WL1 W2 pH Valve V9 pH V9H pH e W3 Outlet Valve V9 O V9H O e W 108 AKTA avant 29 1015 56 AA 5 5 2 4 2 3 70 Frac
94. 2 N 2 1 15 2 2 24 2 3 27 2 4 31 14 AKTA qvant 29 1015 56 AA 2 2 1 21 15 9 16 17 78 20 4 2
95. kk 74 4 2 6 77 LIAZ PIPFOA DL aedddbbbkGQGG GG 78 44 UNICORN LL 2g 79 45 ee 82 4 5 7 44 83 4 5 2 Sogmp e Pump 2 97 45 3 0 96 4 6 101 5 cucina 102 5 1 103 5 2 ee 105 5 3 44 110 2 AKTA qvant 29 1015 56 AA 5 4 ec 111 5 5 ee 116 56 pH HH 118 5 7 120 5 8 126 6 ii 127 6 1 2 2 ee 128 6 2 _ eee 131 6 3 kk 134 6 4 ee 140 6 5 143 ee 146 71
96. Fraciion Collector Conient System3 4 5 8 9 10 LEI A A A A IL PIE a ee a P ld lds 7 A Ee cw ee Eh Eh kh 4 4 50 ml tubes 6 deep_wellplate_ m w 15 mltubes 4 5 D 0 IET TLNOd O1 11OO NOILO V AKTA avant 29 1015 56 AA 125 5 5 8 5 8 AKTA avant AK7A avant User Monuo 2 AKTA avant AKTA ava
97. N A AKTA avant AKTAgvant AKTA ovgnt UNICORN
98. Select Columns System3 Enter or select a calumn Individual Code lat exp ID Enter ID ks 9288 13 12345678 nnnn nn ooos SelectID 1234 000 00 O005 HiPren 261 Disable calumn logging for this un Apply to all methods with the same column type hiethods Hemark Calumn Earcode Calumn tpe Coumn Handing 28 9288 13 12345678 D000 00 O005 HiPren 26 10Desaling Column Handling Scouting ruh 2 HiPrep 26 10 Desalting Calumn Handling Scouting run 3 HiPrep 265410 Desalting SelectlD Select Columns System3 Enter or select a column Individual Code Enter ID Select ID 28 d288 13 12345B78 ODDOD OOD O005 HiPrep 2610 Desalting E Disable column logging 28 3288 13 12345B78 OOOOD O0 HUB HiPrep Desalting 2610 E 28 9288 13 28928813 ODDOD OD 1234 HiPrep 2610 Desalting E Applp to all methods with the same column type klethods Riemark Column Barcode Column type Column Handing 28 3288 13 28928813 ONO0 00 1234 HiPrep 26 10Desaling Calumn Handling Scouting run 2 HiPrep 26 10 Desalting Column Handling Scouting run 3 HiPrep 26 10 Desalting OK
99. lt st a Fo DD 10 7 5 172 AKTA avant 29 1015 56 AA 165 7 7 3 Pump P9 5 0O 0 1 166 0O 0 3x1 mm WI WAM 0 3x1 mm 56686700 20
100. 2 1 2 NID I 8 AKTA qvant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H 0 8 9 10 Z 5 T 7
101. Inlet valve A Al A2 3 ng A a ng process Picture Sysrem pumps s Conc B 0 B Set B System flow lt 1 000 mlmin Setflowrate Conc B 0 0 B Set B Pump wash A B A1 rv Start pump A wash B1 Siart pump B wash Q1 Start Q inlet wash System wash System out WwW Injection valve W1 15 ml Start system wash System Pump A AKTA avant 29 1015 56 AA 89 4 4 5 4 5 1 System PumpA B Sample Pump 1 Process Picture Injection valve Manual load
102. 1 ii AN Sysrem CJP Column C P AKTA qvant 29 1015 56 AA 6 6 1 v Co umn CIP AN End Run Log Sysre Control Hold Pause Eng Connue
103. UV 0 UV 7Z 5 172 0o 7 2 Pump P9 Pump pgJ 0 150 7 3 Pump P9 5 0 167 AKTA avant 29 1015 56 AA 7 AL 7 4
104. ee 147 7 2 Pump P9 Pump P9H 0 2 150 7 3 Pump P9 5 O oe 161 74 ka ka 170 7 5 172 8 175 S81 OhhGhhAAGGGGhNNNb AA 176 8 2 ee 178 8 3 D kk 183 RI Al A 187 AKTA qvont 29 1015 56 AA 3 1 1 AKTAavant 1 1 5 1 2 6 1 3 8 1 4 12 4 AKTA qvant 29 1015 56 AA 1 1 1 1 1
105. 15 15 27 54 24 15 16 8 9 176 27 28 176 178 134 140 143 B 83 B 83 5 17 18 8 18 144 33 36 126 AKTA avant 29 1015 56 AA 71 109 109 16 7 AKTA qvont 29 1015 56 AA
106. O AKTA avant 29 1015 56 AA 7 7 3 Pump P9 5 0O 1 2 AKTA qvont 29 1015 56 AA 2 M 2 1 2 163 7
107. 2 1 A GE 2 AKTA avant AKTA qvant
108. Open Method Navigator Method Navigator Method Navigator mx Methods Method 5 DoeMethod vs 2012 11 27 14 5 Default AutoTests 2013 05 31 2013 05 31 12 0 Default Evis1 2012 11 27 14 5 Default 2 Aun Start Protocol 3 Start Protocol Aex 134 AKTA qvant 29 1015 56 AA 6 6 3 4 Start Proroco Start UNICORN Se ectCo umns Select Columns System3 Enter or select a column Individual Code Enter ID SelectID
109. 770 AKTA avqnt 29 1015 56 AA 149 7 7 2 Pump P9 Pump P9H 0 4 7 2 Pump P9 Pump P9H O0 O0 P9 P9H A P9H B P9H S 0
110. Log On AKTA qvont 29 1015 56 AA 79 4 44 UNICORN 2 Lo9 0n User Name se Password Use Windows Authentication User Name Log On UNICORN FF Use Windows Authentication User Name Default Domain Access AccessToEventhina vr Group Start Administration System Contml F Method Editor IF Evaluation a es Cana OK UNICORN 80 AKTA qvant 29 1015 56 AA 4 44 UNICORN UNICORN 7 1 Sysre Control Connectto Sysfems Connect to Systems Connect to Systems Connected sustems 1
111. pH pH N pH pH pH s pH pH pH pH pH AKTA qvont 29 1015 56 AA 73 4 4 2 42 5 4 Z 5
112. pH AK7A avant User Manual Chapter Mojntengnce AKTA qvont 29 1015 56 AA 119 5 5 7 5 7 AK7A avantUser Monuo NJ RPC F9 R A
113. 2 Outlet Valve V9 02 V9H 0O2 12 21 3 Outlet Valve V9 03 V9H 0O3 12 32 External Air Sensor L9 1 5 L9 1 2 O box E9 2 UV Monitor U9 L 280nm UV AKTA qvant 29 1015 56 AA 3 3 1 AKTA avant 2 Conductivity Monitor C9 2 Fraction Collector 175 CF9 R Reggy
114. 35 3 3 1 AKTA qvant 36 AKTA avont AKTA avaqnt 25 AKTA qavant 150 AKA avant 25 AKA avant 150 AKTA avant 25 50ml AKTA avant 150 AK7A avant 25 AKTA avant 25 AKTA avant 150 System Pump A system Pump B Sample Pum
115. Highalarm e Click Execurfe AKTA qvont 29 1015 56 AA 117 pH 56 pH pH pH 1pH 2 pH ek pH pH 4 pH7 2 pH i pH pH 118 pH 1 Sysre Conro Sysrem Calibration
116. 75cm 52 AKTA qvant 29 1015 56 AA 4 4 1 4 1 2 AKTA qvant 2x30cm 80 cm AKTA qvont 29 1015 56 AA 53 4 4 1 4 1 2 20c m AN AKTA avqnt 20 cm
117. PEEK 0 75 mm AKTA qvant 29 1015 56 AA 5 2 5 5 2 N 10 40 kg
118. System Control File Edit View Manual System Toos Help Ip12 047 Run Data gt x a System state Acc volume System flow Sample flow PreC pressure UV1_280 UV 2nd pH a Manual Run 0 862 ml 1 000 mmin 0 000 mlmin 0 22 MPa 0 01 MPa 13 746 mAU 43 825 mAU Of system flow 1 000 mi min 0 0 sg sample fow 0 000 Manual load PreC 0 22 Mpa cond 0 00 ns cm pH Off Temp 20 2 c High alarm 2 00 V1 13 mAU ms Al mm A a Ew1 Co Waste SN a urent OTTO Pm MVM mSyP F _ By pass em UV mm COnd mm RestrictOr mm Frac 5 lt 32S i 4 gt B8389 B1 B 1 DeltaC 0 01 Mpa gh alarrr 20 00 Buffer S Wi mponents without a fixed place 3 43 825 mau 2nc O box Manual Run Bloclc No watch onnection onn ntrol Controlled by Default HCE C8ZH05J Mew RunLog Run Log 46 AKTA qvant 29 1015 56 AA 3 3 2 UNICORN 3 2 2
119. erA Inlet B InletValveB 7 B7 B6 B1 B1 Inlet valve B B2 B3 B4 B5 B6 B7 25 30 ml System Pump B CS I ly M 3 4 3 6
120. 9 AKTA avant 29 1015 56 AA 15 2 2 1 AN GE AKTAavant AKTA avant A AKTAqvqnt
121. UNICORN UNICORN 78 AKTA avant 29 1015 56 AA 4 44 UNICORN 44 UNICORN UNICORN UNICORN UNICORN UNICORN e license e license UNICORN Administration and Technical Manual 1 UNICORN og 0n UMCORN Log On sysre Control Start System Control
122. Calibration Galibration System3 Monitor to calibrate Currert value Calibration procedure Prepare far calibration Calibration for pH electrode buifer 1 Description pH calibrates the pH electrode with two point pH for buffer 1 i calibration i e using twn pH calibration solutions It can also be used to fill the pH cell with storage solution since the pH yalye is in calibration position pH for buffer 2 For calibration preferably use pH standard Pe or 7 as the first calibration point and a buifer close to Ihe lowest or hghest HH Last calibrated nn 2009 01 13 10 52 07 01 00 you need to measure as your second noin Calibrated electrode slope 391 Farameters pH for buffer 1 Assymetry potential at pH 7 my PH for buffer 2 AKTA qvant 29 1015 56 AA 5 5 6 pH 2 Monitor to calibrate pH pH 3 Prepare for calibration pH 4 pH pH pH for buffer 1
123. AKTA qvont 29 1015 56 AA 85 4 4 5 4 5 1 2 Process Picture Injection valve System pump waste SysteTi ump Wnste LoopE Loop valve prec system pump By pass wraste rn syp Inrechnn yahye Direct inject Sample pump waste aa 3 process Picture se etvg veB s 1 Inlet walve B 3 86 AKTA avant 29 1015 56 AA 4 4 4 5 4 5 1 process P
124. 1m 7 8 System PumpB 6 8 Uy Py 9 System Pump A B Sample Pump 90 88 AKTA qvant 29 1015 56 AA 4 4 5 4 5 1 System Pump A System Pump B 85 3 4 System PumpA 3 process Picture etvg eA s 1
125. 52 12 pH 144 144 144 11 5 189 www gelifesciences com contact GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden WwWww gelifesciences com avant GE GE General ElectricCompany AKTA General Electric Company BD Becton Dickinson qnd Company Decon Decon Laboratories Limited Eppendorf Eppendorf AG Microsoft Windows Microsoft Corporation NUNC Thermo Scientific Thermo Fisher Scientific SARSTEDT SARSTEDT AG amp CO Seqhorse Bioscience Seahorse Bioscience Inc Triton Union Carbide Chemicals qnd Plastic Company Inc Tween Uniqema Americqs LLC VWR VWR International LLC UNICORN
126. Evaluation C gssjc Administration Method Editor System Control Evaluation Classic F1 AKTA avant 29 1015 56 AA 45 3 3 2 UNICORN 3 2 2 3 2 2 System Conro System Contro 3 sysre Control Run Data 1 Chromatogram 2 Process Pycture 3
127. s A 10 s 2 B D13 A 1 B 3 3 D D 4 D 5 186 AKTA qvant 29 1015 56 AA A AKTA qvant 120 AKTA qvant 176 C CE 8 CE 9 F FCC 10 P pH pH 144 U UNICORN 44 81 46 79 79 78 116 144 144 1 59 AKTA avant
128. 146 SystemC P Co umn C P I AN System CIP 1 NaOH AKTAavant User Ma
129. Flow rate 25 0 ml nserr Click Execute 6 2 7 160 AKTA avant 29 1015 56 AA 7 7 3 Pump P9 5 0O oO a O O AM AM 7 3 Pump P9 S O Pump P9 S O 0 2
130. MAorconnecreg 10 UMCORN UNICORN 4 UPS UPS AKTA avant UPS 176
131. System pump waste Inject Sample pump load Direct inject Sample pump waste 2 3 Chromatogram PreCpressure PreCpressure AK7A gvont User Monug System Control End 90 AKTA avant 29 1015 56 AA 4 4 5 4 5 2 Sqample Pump 4 5 2 Sample Pump 1 2 3 4 5
132. TAKTAavant state Ready pase IL Continue UNICORN System Conro AKTA avant 29 1015 56 AA 41 3 3 1 AKTA qvant e Wash e Pause e Hold 42 AKTA qvant 29 1015 56 AA 3 32 INICORN 3 2 UNICORN UNICORN Sysre Conrro System Control Administration Method Editor Evaluation 3
133. GE 4 2 1 65 4 2 2 66 4 2 3 70 4 2 4 Bqrcode Scanner 2 D pH 73 4 2 5 74 4 2 6 6 64 AKTA avant 29 1015 56 AA 4 42 4 2 1 4 2 1 AKTA avant
134. Iniect sample from loop Fill the loop using Manual load Y Loop tupe Capillary loap wv ml Inject sample directly onto column 7 Sample inlet Fill laop wi ml Empty loop with 1 001 ml Sample wolume ml Use the same inlets as in Method Settings Inlgt A1 IletB 1B1 oo Fil the swstem with the selected bulfer AKTA qvant 29 1015 56 AA 133 6 6 3 6 3 Co umnLog5ook UNICORN Method Mgnuo 1 System Conrro
135. UNICORN 3 2 1 UNICORN 44 3 2 2 46 AKTA qvont 29 1015 56 AA 43 3 3 2 UNICORN 3 2 1 UNICORN 3 2 1 UNICORN UNICORN UNICORN UNICORN 6 4 44 AKTA qvant 29 1015 56 AA 3 3 2 UNICORN 3 2 1 UNICORN UNICORN UNICORN 4 Administration Method Editor System Control Evaluation Adm
136. 5 2 Mgnuo instructions e Pumpsand pressures Quaternary start concentrations StartconcentrationQ1 100 0 Manual instructions System3 Selected column type select Instruclians F Parameters for Quaternary start concentrations Pumps and pressures 2 Start concentration Q1 oO 100 0 System flow 3 Sample Flow 100 0 Gradient Start concentration 2 0 100 0 Pump wash 3 gt Sustem wash D0 EE start concentrations Start concentration 3 0 100 0 uaternary gradient EE BuifferPro pH 00 Column packing flow Start concentration 4 Hi 1 Flow path a 1 HH Monitors D0 Save result as uto update of parameters during run Pumps and pressures System flow Flow rate 0 01 ml e Click Execute 3 25 30ml Ee AKTA avant 29 1015 56 AA 97 4 4 5
137. 29 1015 56 AA 111 5 5 4 2 1 112 AKTA avant 29 1015 56 AA 5 5 4 3 1 1A 4 process Picture se Columnvalve Position 1 Down flow OO Down flow CO Up flow Set position Position 10 1 AKTA qvont 29 1015 56 AA 113 5
138. US 2m EU 2m Power 100 240 VAC 50 60 Hz 68 AKTA avant 29 1015 56 AA 4 42 4 2 2 1 LAN AKTA 2
139. Disable calumn logging for this run amp pply to all methods with the same calumn type Melhads Femark Calumn Barcode Calumn type Column Handling Scouting run 2 HiPrep 26410 Desalting Column Handling Scouting run 3 HiPrep 26 10 Desalting s UNICORN AKTA qvont 29 1015 56 AA 6 6 3 oO 1 2 5 Select Columns System3 Enter or select a cnlumn Individual Enler ID Select ID OO Disable column logging for this run Applp to all methods with the same column type klethods Hemark Column Barcade Calumn type Lalumn Handling Scouting run 1 I HiFrep 25 10 Desalting Lalumn Handling Scouting run 2 HiPrep 265410 Desalting Column Handlin
140. 50 ml 20 1 5 4 4 2 4 2 5 10 11 12 25 30ml 25 30ml
141. 29 1015 56 AA 121 5 5 7 QuickRelease 4 122 GE ESSs AKTA qvant 29 1015 56 AA 5 5 7 GE 2 gt 2 lt lt 424 lt a 3 lt lt lt MS PX gt v 4
142. 6 7 3 33 5 8 System Pump4 8B Sompje Pump 90 System Control Eng 100 AKTA qvant 29 1015 56 AA 4 6 AKTA avant AKA avant User Manual AKTA qvont 29 1015 56 AA 101 5 5
143. AKTA avant 29 1015 56 AA Injection valve Sample pump waste W1 Col A SyP F Ws rs Injection valve rd Manual load System pump waste Inject Sample pump load Direct inject Sample pump waste 93 4 4 5 4 5 2 Sqample Pump 5 Process Picture Sample inlet 8u7er Sample pump Sample flow 1 0 AKTA avant 25 ml 5 0 AKTA avant 150 ml Inlets Pumps Sample pumpwaste mk A1 le A E Column 1 je Col 3 3 P M syp F N Bl B Sempleflow 9 EE Buffer mam S ah Buffer Start pump wash Setflow rate 6 25 30 ml
144. gt AKTA avant 29 1015 56 AA 71 4 42 4 2 3 N AKTA qvant 25 2 10 AKTA qvant 150 40 72 AKTA qvant 29 1015 56 AA 4 4 2 4 2 4 Barcode Scanner 2 D pH 4 2 4 Bdarcode Scanner 2 D pH Bqrcode Scqnner 2 D pH Barcode Scanner 2 D USB pH
145. 12125 02 9 235 186 4 7783 20 2 231 984 1 107 21 1 203 473 3 56 81 5 200 289 5 AKTA avant 29 1015 56 AA 181 8 8 2 182 CAS no EC no 74 79 3 200 811 1 100 51 6 202 859 9 DTE 3483 12 3 222 468 7 DTT 3483 12 3 222 468 7 EDTA 60 00 4 200 449 4 37482 11 4 253 523 3 7447 40 7 231 211 8 CAS no EC no 67 64 1 200 662 2 7664 41 7 231 635 3 DMSO 67 68 5 200 664 3 75 08 1 200 837 3 7664 38 2 231 633 2 AKTA qvant 29 1015 56 AA 8 83 D ID
146. 5 10 ml pH pH Cal Luer 6 Current vg ue Calibrate pH Cal pH 8 2 pH pH pHfor bufer2 9 2 pH 5 6 Calibrated electrode slope Asymmetry potentialat pH 7 10 Calibrated electrode slope gt 80 Asymmetry potential at pH 7 60 mV Close pH Calibration pH pH
147. Flow path Inlet 8 System Pump B Position Insert Pumps and Pressures Gradient Target 0 B System Pump A 100 B B Pump P9 Pumps and Pressures System flow Flow rate 5 0 ml sert Pump P9H Pumps and Pressures Syste flow Flow rate 25 0 ml nsert Click Execure Manualiinstructions Flow path Column vo ve nsert Flowpath Sampleinlet Position nsert Flow path Injection valve Position Direct mect nserr Pumps gnd Pressures Sample flow
148. Quaternary Valve Ouqternary Valve Quaternary Valve System Pump A System Pump B 98 AKTA qvant 29 1015 56 AA 4 4 5 4 5 3 O 1 Manualiinstructions s Pumps and pressures Pump wash Bu7 7erPro 0 inlets 4 Manual instructions System3 Selected column type select Instruclinns a Parameters for Pump wash Pumps and pressures Inlet amp System f
149. 2 2 1 Sysrem CIP Co umn CIP 1M NaOH AKTA qavant UV NaOH pH pH 1
150. 29 1015 56 AA 77 4 4 3 4 3 UNICORN UMCORM Administration gn Technical Mgnug UNICORN UNICORN UNICORN UNICORN UNICORN E licenses UNICORN Process Picture Windows
151. 29 1015 56 AA AKTA avant 120 103 144 66 25 UNICORN 79 47 141 46 44 45 D ID 186 ID 186 183 62 62 Oo 141 172 83 pP9 S 161 P9 P9H 150 170 187 188 147 22 XX WC x JI Nl 6 MI NII YN 25 25 31 31 116 18 50
152. 4 1 3 4 1 3 AKTA qvant 4 C 35 C 25 C 60 C 20 95 2 000 m 2 AKTA qvant 800 W 300W 1100 W 56 AKTA qvant 29 1015 56 AA 4 1 4 4 4 1 4 1 4 AKTA avant A 4 I
153. ETSI EN 300 330 2 EN EU ERM 1999 5 EC SRD 9 kHz 25MHz 9kHz 30 MHz FCC FCC 15 2 1 2 GE FCC 15 A
154. P9 B P9H B Sample Pump P9 S P9H Pressure Monitor R9 System Pump A System Pump B Inlet Valve A V9 IA V9H IA AKTA qvant 25 3 06 ml 1 4 ml 5 ml AKTA avant 150 3 1 4 ml 5 ml 15 ml 15 ml AKTA qvqnt 25 15 ml 5 MPq 50 7
155. PumpP9H 2 3 MPq 20 30 e Pump P9 P9 Seal kit 25 ml Pump P9H P9H Seal kit 150 ml p9 P9H Pump P9 AKTA qvont 29 1015 56 AA 151 7 7 2 Pump P9 Pump P9H O Pump P9H O 1 2 152 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H 0 3 2
156. 1 1 4 1 4 AKTA avqnt AKTA qvant AKTA qvqnt AKTA avant Unpacking Instruction AK7A avant AKTA avant User Manual AKTA avant 25 Product Documentation AKTA avant 150 Product Documentation 1 UNICORN UNICORN Help UNICORN F1 UNICORN Online Help and Documentation J UNICORN Help UNICORN Help O 12 AKTA q
157. 193 8 8 3 DD ID Column Valve 2 Column Valve N A pH Valve V9 pH V9H pH pH Monitor H9 pH p Outlet Valve V9 O V9H O UV Monitor U9 M 0 UV U9 D 0 Conductivity Monitor C9 0 ID ID ID 2 Inlet Valve A V9 A2 12 V9H A2 2 Inlet Valve B V9 B2 13 V9H B2 Inlet Valve 1 V9 IX V9H 15 IX Inlet Valve 2 V9 IX V9H 16 IX 184 AKTA qvant 29 1015 56 AA 8 8 3 D 2 Sample Inlet Valve Versatile Valve V9 S2 V9H S2 V9 V V9H V ID 14 2 Versatile Valve V9
158. 215 185 5 1M 40 N A 7647 14 5 231 598 3 7681 52 9 231 668 3 1 HCI HCI 0 1M AKTAqvontn V9 C 1MHCI 178 CAS no EC no 50 01 1 200 002 3 151 21 3 205 788 1 SDS TRITON 100 9002 93 1 Tween 20 9005 64 5 500 018 3 57 13 6 200 315 5 180 AKTA avant 29 1015 56 AA 8 i ds RPC CAS no EC no 100 75 05 8 200 835 2 859 15 109 99 9 203
159. 5 4 5 Process Picture s System pumps Sysrem flow 0 2 ml Setflow rate Syslem flnw DU BB et 35 ls re aware ei 0 2 ml 6 1A 1A 114 AKTA qvant 29 1015 56 AA 5 5 4 7 1 8 2B 9 system Control End AKTA qvont 29
160. Desahing alumn type HiPrep 26 10 Desaling Use medium batch ID AKTA avant 29 1015 56 AA 137 6 6 3 4 New Co umn Alias Technigue Coumnype Set medium expiration ggfe OK 138 AKTA qvant 29 1015 56 AA 6 6 3 5 Se ecr Co umns EnterID ID Borcode Scanner 2 D 3
161. 1015 56 AA 115 5 5 5 5 5 2 Column Valve V9 C V9H C VM MonitorU9 2 Conductivity Monitor C9 UV Monitor U9 L 2 MPa
162. V V9H V 3 Versatile Valve V9 V V9H V 3 Versatile Valve V9 V V9H V Loop Valve V9 L V9H L 2 Column Valve V9 C2 V9H C2 2 Column Valve N A 2 Column Valve N A 2 Outlet Valve V9 02 V9H 02 3 Outlet Valve V9 03 V9H 03 External Air Sensor O box L9 1 2 L9 1 5 2 O box 2 UV Monitor 2 Conductivity Monitor 2 Fraction Collector AKTA avant 29 1015 56 AA 8 8 3 D ID ID 2 ID Ee 1 A4K7A avant User onug 2 ID 2
163. selected max 3 System name Control Wiew Systemd Systems SystemE Systemr 2 Connect to Systems Control OK UNICORN AK7 avant User Manual AKTA qvont 29 1015 56 AA 81 4 4 5 45 0
164. 1015 56 AA 5 5 7 4 N Pguse sSysreconro Wew Fraction Collector Confent
165. 20 2 Conductivity Monitor C9 5 MPg 50 2 UV UV UV 2 MPa 20 5 MPa 50 2 UVMonitorU9 L 2 Conductivity Monitor C9 UV MonitorU9 L 2 0MPaq 20 2 Conductivity Monitor C9 5 0 MPa 50 116 AKTA avant 29 1015 56 AA 5 5 5
166. 5 4 5 3 O 4 5 3 O 1 0 2 Q 3 Quqternaryvqlve 4 Quarternary Volve 5 O O 1 A1 B1 Q1 Q4 A1 B1 96 AKTA qvant 29 1015 56 AA 4 4 5 4 5 3 O
167. 7 3 Pump P9 5 0O 6 A CN WV ie D 4 IN 4 M 8 2 164 AKTA qvant 29 1015 56 AA 7 7 3 Pump P9 5 0O 9
168. 72 AKTA qvant 29 1015 56 AA 155 7 7 2 Pump P9 Pump P9H 0 0 1 O 0 0 3x1 mm 56686700 2 20 P9 P9H AKTA qvant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H 0 3 2 4
169. 726 8 N A TFA lt 0 2 TFA 100 75 08 1 200 837 3 100 67 63 0 200 661 7 100 74 93 1 200 659 6 5 N A 1 PEEK 4 AK7A gvon User Monuo 2 0O 29 0113 26 HIC CAS no EC no
170. 74 AKTA qvant 29 1015 56 AA 4 42 42 5 74 1 AKTA avant 29 1015 56 AA
171. AKTA avant RL OE OS 4 5 1 2 LL 6 1 3 8 11 5 1 12 2 kk i A f A ac 14 21 8mW gt KTmXmx2 IKIE OO ONOI SI u 15 2 ec GGGGc iC 24 23 kk 27 24 ee 31 3 KR KR RRRK KAKQQ QOL 32 3 1 AKTA qavant ec 33 20 UNICORN ec illA As 43 3 2 1 UNICORN 44 3 2 2 Ek 46 48 41 ee 49 4 7 7 5 50 4 1 2 52 56 4 7 4 5 4 7 5 59 476 kk 61 42 64 4 2 1 4 65 4 2 2 66 42 3 kk 70 42 4 Borcoge Scanner 2 D pH Lz L 73 4 2 5
172. etwork LAN 66 AKTA qvant 29 1015 56 AA 4 4 2 4 2 2 UniNet 9 UniNet 9 UniNet 9 UniNet 9 UniNet 9 AKTA qvont 29 1015 56 AA 67 4 42 4 2 2 AKTA aqvaqnt AKTA avant 1 2
173. g Scouting run 3 HiPrep 265410 Desalting ww ce 2 Se ecr Co umns AMew New Co umn New Canlurmn Code Int BRD ID mmp The Calumn has a uriTag Ihas a lined Codes and exp nnhinug Cahnce AKTA avant 29 1015 56 AA 6 6 3 3 Borcode Scqnner 2 D Coge s Borcode Scanner 2 D ID ID s Continue New Co umn New Column Eaulumn ID mlias optional Technique
174. icture Conc 8B 100 B Set B e System pumps System flow 1 000 mMmn Setfowrae ooo ze 0 100 Pump wash A B A1 Start pump A wash B1 Start pump B wash Qi v Start Q inlet wash System wash System out W Injection valve W1 15 m Start system wash 10 999 Sysre flow AKTA qvant 25 1 0 ml AKTA avant 150 5 0 ml se Setflow rate System Pump B 25 30 ml System Pump B FT TI tt oe 8 8 Ne AKTA qvont 29 1015 56 AA 87 4 4 5 4 5 1 6 3 4 5 10 ml
175. inistration Method Editor 1 System Control Process Picture Evaluation Evo ugion Design of Experiments
176. letVvalveA Inlet Valve B V9 A2 V9 B2 V9H A2 V9H B2 14 System Pump A System Pump B 2 Inlet Valve X1 Inlet Valve X2 V9 IX V9H IX 8 2 Saqample Inlet Valve V9 S52 V9H S2 14 Saqmple Pump 2 Versatile Valve V9 V V9H V 4 4 Loop Valve V9 L V9H L 5 2 2 Column Valve V9 C2 V9H C2 5
177. low Sample flow Of Gradient Inlet B Fump wash System wash ff 0 Quaternary start concentrations BufferPro 0 inlets Quaternary gradient BufferPro pH MM amp 2 Calumn packing flow Sample inlet Flow path HH Monitors wy Hf Sawe result as Bewse uto undate of parameters during run e Click Execute 0 Quaternary Valve 2 3 Pumps and pressures System flow Flow rate 0 01 ml 4 25 30 ml AKTA avant 29 1015 56 AA 99 4 4 5 4 5 3 O 5 3 4 10 ml 1 ml
178. m 1 16 PEEK 0 50 mm 1 16 ETFE 10mm 1 16 259C 60 C 82 178 176 AKTA qvant 29 1015 56 AA 8 8 1 A 20 95 AKTA avant lt 70 dBA AKTA avqnt 29 1015 56 AA 177 8 i 8 2 AKTA avart AKTAavont PEEK
179. nt AKTA qavant AKTA avqnt Ethernet
180. nual pH AK7A avant User Mgnuog AKTA qvont 29 1015 56 AA 143 6 6 5 2 3 System CIP 20 Column CIP 1 2 Column CIP 20 pH pH 1 pH PH pH
181. p Pressure Monitor Mixer Injection Valve Quarternary Valve Inlet Valve A Inlet Valve B Sample Inlet Valve Column Valve pH Valve Outlet Valve UV Monitor Conductivity Monitor Built in fraction collector AKTA avant 29 1015 56 AA 3 3 1 AKTA qvqnt AKTA avant 25 AKTA avant 150 2 Inlet Valve A 2 Inlet Valve B Inlet Valve X1 Inlet Valve x2 2 Sqmple Inlet Valve Versatile Valve Loop Valve 2 Column Valve 2 Outlet Valve 3 Outlet Valve External Air Sensor L9 1 5 External Air Sensor L9 1 2 O box 2 UV Monitor 2 Conductivity Monitor 2 Fraction Collector Quaternary Valve Q9 4 System Pump A PA P9H A AKTA qvont 29 1015 56 AA 37 3 3 1 AKTA qvant 38 System Pump B
182. qvont 29 1015 56 AA 1 4 4 1 4 1 2 4 1 2 AKTA avant I AN GE s
183. vant 29 1015 56 AA Gettind started with Evaluation UNICORN 7 0 1 1 4 Evaluation Evaluation UNICORN Method Manual UNICORN UNICORN Administration and Technical Manualt UNICORN UNICORN UNICORN Evaluation Monuo UNICORN Evaluation Classic UNICORN UNICORN Sysre Control Manualt UNICORN 1 UNICORN AKTA qvont 29 1015 56 AA 13 2
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