ÄKTA avant Operating Instructions
Contents
1. Superloop Superloop Syr Superloop System CIP CIP ColumnCIP CIP2. GE AKTA avant AKTA avant 25 2 10 AKTA avant 150 40 30 cmis
3. 2 1 14 2 2 22 2 3 25 2 4 28 2 5 DoHS 29 AKTA avant 29 1015 56 AA 13 2 2 1 2 1 14 15 16 16 18 20 ht AKTAavant
4. 20 C System Control System Settings Method Editor Text Instructions AKTA avant 29 1015 56 AA 117 118 6 1 119 2 121 6 3 123 6 4 129 6 5 132 AKTA avant 29 1015 56 AA 6 6 1 6 1 J J1 PPE
5. PPE AKTAavant AKTAavant AKTA avant 29 1015 56 AA 2 2 1 AKTA avant 116
6. P9 S O 148 AKTA avant 29 1015 56 AA 7 7 3 Pump P9 S O T20 20 Ref 1 Pp9 S Sealkit 65 ml Pump P9 S 0 AKTA avant 29 1015 56 AA 149 7 7 3 Pump P9 S O 1 2
7. This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Products The Number in the symbol is the Environment friendly Use Period EFUP which indicates the period during which the toxic or hazardous substances or elements contained in electronic information products will not leak or mutate under normal operating conditions so that the use of such electronic information products will not resultin any severe environmental pollution any bodily imjury or damage to any assets The unit of the period is Year In order to moaintain the declared EFUP the product shall be operated normally according to the instructions and environmental conditions as defined in the product manual and periodic maintenance schedules specified in Product Maintenance Procedures shall be followed strictly Consumables or certain parts may have their own label with an EFUP value less than the product Periodic replacement of those consumables or parts to moaintain the declar
8. T20 Pump P9 P9H 7 2 Pump P9 Pump P9H 0 139 7 2 Pump P9 Pump P9H 0 139 AKTA avant 29 1015 56 AA 155 7 7 4 Pump P9 S Pump P9 S 7 3 Pump P9 S 0 148 156 AKTA avant 29 1015 56 AA 7 7 5 7 5 Dm DY
9. 9 10 90 11 AKTA avant 29 1015 56 AA 159 AKTA avant AKTA avant 25 Product Documentation AKTA avant 150 Product Documentation 8 1 161 8 2 163 8 3 ID 167 AKTA avant 29 1015 56 AA 38 1 8 8 1 UNICORN 6 0 PC x x 860 x 710 x 660 116 100 C 240 C VAC 50 C 60 C Hz 800
10. PPE System CIP CIP Column CIP CIP System CIP CIP Column CIP CIP NaOH AKTA avant 29 1015 56 AA 119 6 6 1
11. Superloop Superloop Manuoal instructions AKTA avant AKTA avant AKTA avant AKTA avant UV UV 2 MPa 20 bar si 5 MPa 50 bar AKTAavant
12. 100 AKTA avant 29 1015 56 AA 163 8 8 2 MSDS MSDS CAS no EC no CIP 2 CAS no EC no 75 05 8 200 835 2 Decon 90 75 08 1 200 837 3 67 56 1 200 659 6 7647 01 0 231 595 7 67 63 0 200 661 7 1310 73 2 215 185 5 1 M 40 4M 7647 14 5 231 598 3 10 7681 52 9 231 668 3 1 HCI HC 0 1 M AKTA avant V9 C
13. 2 M 709 1 M 1 M Column Valve V9 C Column Valve V9 C 1 M BufferPro Quaternary Valve Q9 0 2 M HCl 2 M 10 100 0 2 TFA 5 100 PEEK
14. SJT11363 2006 AKTA avant 29 1015 56 AA 23 2 2 2 24 ISTED Intertek AKTA avant INRTU NRTL 29CFR 1910 7 29 AKTA avant 29 1015 56 AA 2 2 3 2 3 AKTA avant Pause ZKo UNICOR
15. 0 29 0113 26 HIC CAS no EC no 2 M 12125 02 9 235 186 4 3 M 7783 20 2 231 984 1 AKTA avant 29 1015 56 AA 165 8 8 2 CAS no EC no 107 21 1 203 473 3 56 81 5 200 289 5 CAS no EC no 74 79 3 200 811 1 100 51 6 202 859 9 DTE 3483 12 3 222 468 7 DTT 3483 12 3 222 468 7 EDTA 60 00 4 200 449 4 37482 11 4 253 523 3 7447 40 7 231 211 8 CAS no EC no 67 64 1 200 662 2 7664 41 7 231 635 3 DMSO 67 68 5 200 664 3 75 08 1 200 837 3 7664 38 2 231 633 2 166 AKTA avant 29 1015 56 AA 8 8 3 ID 8 3 D 1D
16. Df Fh AKTA avant 29 1015 56 AA 1 1 2 se NE AKTA avant 29 1015 56 AA AKTA avant DoC
17. 4 5 1 4 5 2 Sample Pump 84 4 5 3 Q 89 AKTA avant 29 1015 56 AA SS CS i 6 7 4 45 45 1 System Pump B System Pump B System Pump A System Pump A p Process Picture Manual instructions A B 1 2 3
18. End Run Log System Control Hold Pause End Continue Hold Pause siti Continue Rg End i 120 AKTA avant 29 1015 56 AA 6 6 2 6 2 2
19. Quaternary Valve 1 Quaternary Valve 1 1M NacCl 20 mm mm 3 10 5 11 5 50 56 3 NUNC AKTA avant 29 1015 56 AA 57 4 4 1 4 1 6 EV ER NUNC SARSTEDT Thermo Scientific BD Blosclences VWRTM BD Blosclences BD Blosclences 24 48 96 10 5 2 58 AKTA avant 29 1015 56 AA 4 4 1 4 1 6
20. Pump P9 T20 Pump P9H T10 T20 20 AKTA avant 29 1015 56 AA 139 7 7 2 Pump P9 Pump P9H O Pump P9 6 8 MPa 60 80 ban Pump P9H 2 3 MPa 20 30 bar Pump P9 P9 Seal kit 25 ml Pump P9H P9H Seal kit 150 ml P9 P9H Pump P9 Pump P9H 140 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H O O O 1 2 3
21. AKTA avant User Manual PPE 7 1 136 7 2 Pump P9 Pump P9H O 139 7 3 Pump P9 S O 148 7 4 155 7 5 157 AKTA avant 29 1015 56 AA 135 7 7 1 7 1 AKTA avant AKTA avant User Manual GE AKTA avant
22. Evaluation Cljassic Administration Method Editor System Control Evaluation Cjassic F1 42 AKTA avant 29 1015 56 AA 3 3 2 UNICORN 3 2 2 System Control 3 2 2 System Control J System Control System Control System Control Run Data 1 Chromatogram 2 Process Picture 3 System Control 9 Ime File Edit View Manual System Tools Help _ ID12 047 Run Data XI me Acc volume System flow Sample flow PreC pressure DeltaC pressure UV1 280 UV 2nd pH ual Run 0 862 ml 1 000 mlimin 0 000 mimin 0 22 MPa 0 01MPa 13 746 mAU 43 825 mAU Off 1 In
23. 8 System Pump B 6 8 mm mu 9 System Pump A 8 Somple Pump 1b 82 System Pump A System Pump 8 78 3 4 System Pump A 3 Process Picture Inlet valve A ri Inlet wahre A s2s 43 sags AKTA avant 29 1015 56 AA 81 4 4 5 4 5 1 4 Process Picture System pumps Conc B 0 B Set B System flow 1 000 ml min Setflow rate 1 25 Conc B 00 B Set B 100 i A B Al v Start pump A wash B1 v Starpump B wash Q1 v Start Q inlet wash System wash System out w Injection
24. ne i System flow 1 000 ml min 0 0 5 B sampleflow 0 000 Manual load PreC 0 23 MPa Cond 0 05 ms cm pH Off _ De de Temp 20 5 C Igh alarm UV 1 2 mAU EE Al BH A pg ON sd Pp Vm M mm SyP A UY mm Cond Re Restrictor mm Frac f Bl 8 p gt DeltaC 0 01 Mpa High alarm 0 30 tuffed s S met Pp Available components without a fixed place 43 839 mAU UV 2nd O box 130 AKTA avant 29 1015 56 AA Process Picture Process Picture Imjection valve Injection valve x Manual load System pump waste Inject sample pump load Direct inject Sample pump waste Detailed picture AKTA avant 29 1015 56 AA 131 6 6 5
25. AKTA avant User Manual pH AKTA avant User Manual System CIP 20 AKTA avant 29 1015 56 AA 6 6 5 Column CIP Column CjP 20 pH pH pH pH pH pH By pass pH
26. 100 100 2 3 AKTA avant 29 1015 56 AA 157 7 7 5 4 a Rd gt 2 NS SEE A TIE yw lt AKTA avant 29 1015 56 AA 7 7 5 8 4
27. 4 1 1 a7 4 1 2 49 4 1 3 52 4 1 4 53 4 1 5 55 4 1 6 57 46 AKTA avant 29 1015 56 AA 4 4 1 4 1 1 4 1 1 AKTA avant 116 GE AKTA avant AKTA avant 1000 x 900 x 800 155 x x 25 C 60 C
28. 10 40 kg AKTA avant 29 1015 56 AA 97 5 5 2 x J Pressure Monitor Sample Pump Sample Inlet Valve Quaternary Valve Inlet Valve A Inlet Valve B 7 System Pump A 98 AKTA avant 29 1015 56 AA System Pump B 5 5 2 Pressure Monitor Mixer Imjection Valve Superloop Column Valve UV Monitor Conductivity Monitor pH pH valve Flow Restrictor Outlet Valve AKTA avant 29 1015 56 AA 99 5 5 2 Inject
29. 1 M HCl 163 CAS no EC no 6M 50 01 1 200 002 3 164 AKTA avant 29 1015 56 AA 8 8 2 CAS no EC no SDS 151 21 3 205 788 1 TRITON xX 100 9002 93 1 Tween 20 9005 64 5 500 018 3 57 13 6 200 315 5 RPC CAS no EC no 100 75 05 8 200 835 2 85 15 109 99 9 203 726 8 TFA 0 2 TFA 100 75 08 1 200 837 3 100 67 63 0 200 661 7 100 74 93 1 200 659 6 5 1 PEEK AKTA avant User Manual lt
30. 12 50 20 AKTA avant 29 1015 56 AA 71 4 4 2 4 2 6 4 2 6 1 Power Not connected 2 72 AKTA avant 29 1015 56 AA 4 3 UNICORN UNICORN Administration and Technical Manual NO UNICORN UNICORN UNICORN UNICORN UNICORN
31. RPC F9 R RPC 100 5 MPa 50 bar AKTA avant 25 100 RPC PEEK 0 5 PEEK 10 MPa 100 bar 100 AKTA qvant 150 RPC 100 RPC PEEK 0 75 PEEK AKTA avant 29 1015 56 AA 15 2 2 1 AKTAavant PPE AKTA avant
32. 69 1 2 50 20 3 4 5 70 AKTA avant 29 1015 56 AA 4 4 2 4 2 5 6 25 30 8 9 25 30 10 11
33. M9 AKTA avant 25 0 6 1 4 5 AKTA avant 150 1 4 5 15 AKTA avant25 15 15 5MPq 50 bar Inlet Valve A V9 IA V9H IA Inlet Valve B V9 IB V9H IB System Pump A System Pump B Sample Inlet Valve V9 IS V9H IS Injection Valve V9 Inj v9H Inj Column Valve V9 C V9H C
34. 2015 General Electric Company 2015 3 GE Healthcare GE Healthcare GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue PO Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 29 1015 56 AA 03 2015 a24
35. AKTA avant User Manual 1 Injection Valve LoopF LoopE 2 AKTA avant 29 1015 56 AA 121 6 2 3 4 pe mt at W1 122 Injection Valve Syr Empty oop with Sample Application Phase Properties _ phase Properties Text Instructions iT Sample Application Use the same flow rate as in Method Settings Flow rate 10 000 mmin 0 000 25 000
36. Chromatogram Run Log Flow Scheme System Control 3 2 2 System Control 43 System Control Hold Pause End Continue 1 Pre UNICORN System Control Manual UNICORN ee AKTA avant 29 1015 56 AA 129 Process Picture Process Picture
37. AKTA avant 29 1015 56 AA 153 7 7 3 Pump P9 S O 3 UP 4 5 6 7 Pump P9 S 20 S1 4 5 2 Sample Pump 84 3 Ref1 5 MPa 20 30 bar
38. RPC F9 R RPC 100 5 MPa 50 bar AKTA avant 25 100 RPC PEEK 0 5 PEEK 10 MPa 100 bar 100 AKTA avant 150 RPC 100 RPC PEEK 0 75 PEEK 96 AKTA avant 29 1015 56 AA 5 5 2 5 2
39. 7 3 5 8 System Pump A 6 Somple Pump 82 System Control End 92 AKTA avant 29 1015 56 AA 4 4 6 4 6 DD DY AKTA avant AKTA avant User Manual AKTA avant 29 1015 56 AA 93 5 94 5 1 95 5 2 97 5 3 102 5 4 103 5 5 107 5 6 pH 109 5 7 111 5 8 117 AKTA avant 29 1015 56 AA 5 5 1 5 1 2
40. 54 AKTA avant 29 1015 56 AA 4 1 5 J 4 4 1 4 1 5 AKTA avant PC UNICORN PC PC AKTA UNICORN Service Pack 1 32 64 Windows 7 Professiona VNIlele NE eld 6 GB 6 GB 12 GB 500 MB 3 GB 3 GB 3 GB 2 GB NTFS NTFS NTFS NTFS Windows 7 Professional SP1 32 64 Windows 7 Professional SP1 32 64 Windows Server 2008 R2 64 Windows7 Professional SP1 32 64 Windows7 Professional SP1 32 64 Windows Server 2008 R2 64 1033 1033 1033 1033
41. 2 GE UNICORN Administration and Technical Manual 64 AKTA avant 29 1015 56 AA 4 4 2 4 2 3 4 2 3 A 7 EE DA AA AAT AY v BoernBee EB GD 69 6 ES 6 6 6 6 6 69 pH W W1 W2 W3 AKTA avant 29 1015 56 AA 65 4 4 2 4 2 3 1 pH W W1 W2 W3 3 30 cm
42. 45 76 102 AKTA avant 29 1015 56 AA 5 5 4 5 4 7 AKTA avant 4 tt 5 5 107
43. HD we 6 3 4 8 2 5 9 System Pump A 8 Somple Pump 82 Sample Pump 85 Sample Pump 5 1 2 W2 3 System Control
44. Inlet Valve A System Pump A Mixer Sample Pump Sample Inlet Valve Outlet Valve 34 AKTA avant 29 1015 56 AA 3 3 1 AKTA avant AKTA avant AKTA avant 25 AKTA avant 150 AKTA avant25 AKTA avant150 50 AKTA avant 25 AKTA avant 150 AKTA avant 25 AKTA qvant 25 AKTA avant 150 System Pump A System Pump B Sample Pump Pressure Monitor MIxer Imjection Valve Quarternary Valve Inlet Valve A Inlet Valve B Sample Inlet Valve Column Valve pH Valve Outlet Valve UV Monitor Conduct
45. GE 96 7701 5200 BD Blosclences 353966 Grelner Bio One 780270 Porvalr Sclences 219009 Sedhorse Bioscience S30009 Eppendorf 951033405 0030 501 306 48 GE 7701 5500 Sedhorse Bioscience S30004 24 GE 7701 5102 Sedhorse Bioscience S30024 AKTA avant 29 1015 56 AA 59 60 AKTA avant VY ps a AKTA avant AKTA avant Unpacking nstructions GE i 4 2 1 4 2 2 4 2 3 4 2 4 Barcode Scanner 2 D pH
46. Valve X2 V9 IX V9H IX 2 Sample Inlet Valve Sample Pump 2 V9 S2 V9H 52 14 Versatile Valve V9 V A4 4 V9H V Loop Valve V9 L V9H L 38 AKTA avant 29 1015 56 AA 2 Column Valve V9 C2 V9H C2 2 Outlet Valve V9 02 V9H 02 3 3 1 AKTA avant 12 21 3 Outlet Valve V9 O3 V9H 03 12 32 External Air Sensor L9 1 5 L9 1 2 I O box E9 2 UV Monitor U9 L 280 UV
47. B System Control Process Picture nletA Inlet B Inlet Valve B B7 B6 B1 B1 Inlet vabve B Ba 3 Bd BS Be BY AKTA avant 29 1015 56 AA 77 4 4 5 4 5 1 4 25 30 System Pump B gm 5 3 4 6 7 3
48. pH pH AKTA avant User Manual UNICORN UNICORN UNICORN UNICORN File Log off 4 Log ofFf Eric Exit UMNICORNY UNICORN Log On AKTA avant 29 1015 56 AA 133 6 6 5 UNICORN File Exit UNICORN Log off Eric Exit UNICORN UNICORN UNICORN Yes No Cancel Power 0 134 AKTA avant 29 1015 56 AA 7 7 AKTA avant
49. GE 20 AKTA avant 29 1015 56 AA 2 2 1 AKTA avant AKTA avant AKTA avant 29 1015 56 AA 21 AKTA avant AKTA avant AKTA avant
50. 3 4 5 150 AKTA avant 29 1015 56 AA 7 7 3 Pump P9 S O 6 8 1 T AKTA avant 29 1015 56 AA 151 7 7 3 Pump P9 S O 9 10 7 5
51. 2 Inlet Valve A V9 A2 V9H A2 2 Inlet Valve B V9 B2 V9H B2 13 Inlet Valve X1 V9 IX V9H IX 15 Inlet Valve X2 V9 IX V9H IX 16 Sample Inlet Valve V9 S2 V9H S2 14 Versatile Valve V9 V V9H V 20 Versatile Valve V9 V V9H V 21 Versatile Valve V9 V V9H V 23 3 Versatile Valve V9 V V9H V 24 Loop Valve V9 L V9H L 17 Column Valve V9 C2 V9H C2 6 2 Column Valve 4 2 Column Valve 5 AKTA avant 29 1015 56 AA 2 Outlet Valve 8 8 3 ID V9 02 V9H 02 Outlet Valve V9 03 V9H 03 External Air Sensor L9 1 2 L9 1 5 O box E9 0 box E9 UV Monitor 2 Conductivity Monitor Fraction Collector AKTA avant 29 1015 56 AA 169 8 8 3 ID ID ID ID 1 AKTA ovant User Manual
52. UV 2 MPa 20bar 5 MPa 50 bar UV Monitor U9 L 2 Conductivity Monitor C9 UV Monitor U9 L 2 0 MPa 20 bar 2 Conductivity Monitor C9 5 0 MPa 50 bar System Settings UNICORN Method Manual AKTA avant 29 1015 56 AA 107 5 5 5 108 System Control i ee 1 System Control Manual
53. 2 A1 Al 112 AKTA avant 29 1015 56 AA 5 5 7 3 QuickRelease 4 GE monogram 9 AKTA avant 29 1015 56 AA 113 5 5 7 6 GE monogram 7 50 250 1 50 ml 250 ml 2 114 AKTA avant
54. AKTA avant UNICORN AKTA avant GE AKIAdavant 14 AKTA avant 29 1015 56 AA 2 2 1 nh AKTA avant AKTAavant
55. UNICORN Process Picture Windows UNICORN UNICORN AKTA avant 29 1015 56 AA 73 4 4 4 UNICORN 4 4 UNICORN UNICORN UNICORN UNICORN UNICORN UNICORN Administration and Technical Manual UNICORN Log On UNICORN Log On System Control Start System Control Log On 2 Log On User Name Password Use Windows Authentication Windows User Name 1D 0 Log On UNICORN Use Windows Au
56. 1 AKTA avant 29 1015 56 AA 103 5 5 4 2 3 1 1A Process Picture Column valve Position 1 Down flow en LM Lon Column valve Down flow OO Up flow 1 104 AKTA avant 29 1015 56 AA 5 5 4 5 Process Picture System pumps System flow 0 2 ml min Set flow rate 1 System flow C9 0200 mlimin Conc B Im 00 B Set 5 B 0 100 Dv mn uc 9 0 2 ml min 6 1A 1A AKTA avant 29 1015 56 AA 5
57. 102 5 4 103 5 5 107 5 6 pH 109 5 7 pe ee clea i ER 111 5 8 nn 117 6 0 118 6 1 119 SR 121 6 3 123 6 4 129 6 5 132 OE OO OR 135 7 1 136 7 2 Pump P9 Pump P9H O 139 7 3 Pump P9 S O 148 7 4 155 7 5 157 8 nn 160 CT 161 8 2 163 8 3 1D 167 OTOH 171 AKTA avant 29 1015 56 AA 3 AKTA avant 1 1 5 1 2 6 1 3 8 1 4 1 AKTA avant 29 1015 56 AA 1 1 1 1 1 bolditalic
58. A1 8B1 Ee Manual instructions Pumps and pressures Quaternary start concentrations Startconcentration O1 100 0 Manual instructions System3 Selected column type EB _ Select Instructions PETER Parameters for Quaternary start concentrations S Pumps and pressures Start concentration Q1 0 0 100 0 System flow Sample flow 100 0 Gradient Start concentration Q2 0 0 100 0 Pump wash Tr System wash 00 ee start concentrations Start concentration Q3 0 0 100 0 Quaternary gradient 0 BufferPro pH L 00 Column packing flow Start concentration 4 0 0 100 0 Flow path Monitors 0 01 Save result as _ Browse uto update of parameters during run Pumps and pressures System flow Flow rate 0 01 ml mino Execute AKTA avant 29 1015 56 AA 89 4 4 5 4 5 3 0 3 25 30 4
59. 75 AKTA avant 29 1015 56 AA 49 4 4 1 4 1 2 AKTA avant em 30 0cm EE ee 80 20 AKTA avant 20 I ed 50 AKTA avant 29 1015 56 AA 4
60. System CIP Column CIP 1M NaOH AKTA avant 29 1015 56 AA 2 2 1 AKTA avant UV NaoOH pH pH 1 10 40 kg AKTA avant 29 1015 56 AA 19 2 2 1 gt AKTAavant UV
61. Method Editor A AKIAavant PPE GE pH RPC AKTAavant AKTA avant 29 1015 56 AA 95 5 5 1
62. XXXXXX XX XXXXXTY Sample Code no XXXXXXXX Voltage IXI XXX XXX XXX VAC Serial no Frequency XX XX Hz Mfg Year 2013 Max Power XXX VA Fuse XX X XXAL 250V Protection Class XXXX AE CO kh Merk N3732 MONO GE Healthcare Bio Sciences AB Made in Sweden 751 84 Uppsala Sweden td 61010 1 Cert to CAN CSA Std C22 2 No 61010 1 22 AKTA avant 29 1015 56 AA 2 2 2 28930842 AKTA avant 25 Code no 28924630 Voltage 100 240V Serial no Frequency 50 60 Hz Mfg Year Max Power 800 VA Protection Class A IP21 Wet Side IP22 E jj C Intertek 4001767 GE Healthcare Bio Sciences AB Made in Sweden 751 84 Uppsala Sweden 3 Conforms to ANSI UL Std 61010 1 Cert to CAN CSA Std C22 2 No 61010 1 4 RPC GE
63. 2 ID A B ID 13 A 1 B 3 3 ID ID 4 ID 5 170 AKTA avant 29 1015 56 AA 5 A AKTA avant 111 AKTA avant 161 C CE 8 CE 8 F FCC 9 P pH pH 133 U UNICORN 42 74 74 43 75 14 16 32 35 T 117 AKTA avant 111 UNICORN 74 AKTA avant 29 1015 56 AA 8 107 161 163 28 28 7 1
64. AKTA avant 29 1015 56 AA 37 3 3 1 AKTA avant pH Valve V9 pH V9H pH pH pH pH Valve Outlet Valve V9 0 V9H O UV U9 M 190 700 UV Vis C9 Built in fraction collector 2 Inlet Valve A Inlet System Pump A System PumpB 2 Valve B V9 A2 V9 B2 14 V9H A2 V9H B2 Inlet Valve X1 Inlet
65. phH pH pH pH 1 System Control System Calibration Calibration Calibration System3 Monior to calbrate ETE Curent value Calibration procedure Prepare for calibration Calibration for pH electrode buffer 1 pH for buffer 1 pH for buffer 2 Last calibrated on 2009 01 13 10 52 07 01 00 Calibrated electrode slope Bssymetry potential at pH 7 mw pH calibrates the pH electrode with two point Calibration 1 e using two pH calibration solutions t can also be used to fill the pH cell with storage solution since the pH valve is in calibration position For calibration preferably use pH standard buffers pH 4 or 7 as the first calibration point and a pH standard buffer close to the lowest or highest pH you need to measure as your second point pH for buffer 1 pH for buffer 2 v 1 Generate Report 2 Monitor to calibrate pH pH AKTA avant 29 1015 56 AA 109 5
66. 1A 18 4 5 Manual instructions Flow path Column position Position Insert Flow path Sample inlet Position Insert Flow path Injection valve Position Direct inject nsert Pumps Sample flow Flow rate 25 0 ml min Insert Execute 25 0 ml min 6 2 154 AKTA avant 29 1015 56 AA 7 7 4 7 4 J P9 P9H P9 S 0
67. AKTA avant 4 GE 100 240 VAC 50 60 800 1 3 2 AKTA avant 29 1015 56 AA 53 4 4 1 4 1 4 AKTA avant
68. 1D ID ID ID ID System Pump A P9 A P9H A System Pump B P9B P9HB Sample Pump P9 S P9HS Pressure Monitor R9 Pressure Monitor R9 MIxer M9 Imjection Valve V9 Inj V9H Inj Quaternary Valve Q9 Inlet Valve A Inlet Valve B V9 IA V9H IA V9 IB V9H IB Sample Inlet Valve V9 IS V9H IS Column Valve V9 C V9H C Column Valve Column Valve pH Valve V9 pH V9H pH AKTA avant 29 1015 56 AA 167 8 8 3 ID pH Monitor pH pH Outlet Valve V9 0 V9H O UV Monitor U9 M UV U9 D Conductivity Monitor C9 0 ID ID
69. Flowpath Injection valve Flowpath Injection valve bold Power AKTA avant 29 1015 56 AA 5 1 1 2 1 2 AKTA avant AKTA avant Microsoft Windows UNICORN Administration and Technical Manual
70. X SJ T11363 2006 Data listed in the table represents best information available at the time of publication 30 AKTA avant 29 1015 56 AA 3 3 AKTA avant 3 1 AKTA avant 32 3 2 UNICORN 41 UNICORN AKTA avant AKTA avant 29 1015 56 AA 31 3 3 1 AKTA avant 3 1 AKTA avant AKTA avant AKTA avant User Manual AKTA avant
71. AKTA avant 29 1015 56 AA 85 4 4 5 4 5 2 Sample Pump 4 Process Picture miection valve Sample pump waste pe J WI AAA Co AN en SyP BE Ne m4 Injecbon valve Manual load System pump waste Inject Sample pump load Direct inject Yo 5 Process Picture Sample inlet Buffer Sqmple pump Sample fow 1 0 ml min AKTA avant 25 5 0 ml min AKTA avant 150 Sample pump waste Al gS A SW gt Column SS co P JE M Syp F 7 By pass Bl B Sampleflow Xe Set flow rate Buffer Pump wash Buffer Start pump wash Set flow rate 86 AKTA avant 29 1015 56 AA 4 4 5 4 5 2 Sample Pump 6 25 30
72. Connectto Systems Connect to Systems AKTA avant 29 1015 56 AA AKTA avant AKTA avant AKTA avant Unpacking Instructions 4 1 46 4 2 60 4 3 4 4 UNICORN 74 4 5 76 4 6 93 AKTA avant 29 1015 56 AA 45 4 4 1 4 1 AKTA avant AKTA avant
73. AKTA avant 29 1015 56 AA 137 7 7 1 138 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H O 7 2 Pump P9 Pump P9H O P9 P9H A P9H B P9H S O 0 P9 P9H A P9H B P9H S 0O
74. New Column New Column Column ID lias optionall Technigue Desalting Column type HiPrep 26 10 Desalting Use medium batch ID 126 AKTA avant 29 1015 56 AA 6 6 3 New Column Alias Technique Column type Set medium expiration date OK AKTA avant 29 1015 56 AA 127 6 6 3 5 Select Columns Enter ID Barcode Scanner 2 D ID 3 Select Columns System3 Enter or select a column individual Enter ID OO Select ID Code Iat exp ID Es s288 13 12345678 o000 00 ooos 3 9288 13 12345678 0000 00 0005 HiPrep 261 _ New OO Disable column logging for this run Apply to all methods with the same column type Methods Column Handling Column Handling Column Handling
75. 6 5 132 pH 7 135 System CIP Column CIP System CIP NaOH
76. 157 O 1 3x1 O oO CU Be 3x1 0 56686700 152 AKTA avant 29 1015 56 AA 7 7 3 Pump P9 S O 2 20 3 20 140 2 20
77. 4 AKTA avant 29 1015 56 AA 141 7 7 2 Pump P9 Pump P9H O 5 T20 LT BB 6 Pump P9 T20 PumpP9H T10 0 1 2 142 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H O T20 WLW th 8 9
78. IP 21 IP 22 FEP i d 1 6 5 16 1 8 PEEK 0 75 1 16 PEEK 0 50 1 16 ETFE 10 mm 1 16 25 C 60 C AKTA avant 29 1015 56 AA 8 2 163 161 8 8 1 4 C 35 C 20 95 AKTA avant lt 70 dBA 162 AKTA avant 29 1015 56 AA 8 8 2 38 2 J if ES CS AKTA avant AKTA avant PEEK
79. AKTA avant 25 AKTA avant 150 AKTA avant 25 AKTA avant 150 0 001 25 ml min 0 01 150 ml min Column packing Column packing flow flow 50 300 20 MPa 200 bar 5 MPa 50 bar UV 190 700 nm 190 700 nm 32 AKTA avant 29 1015 56 AA 3 3 1 AKTA avant AKTA avant AKTA avant 25 AKTA avant 150 AKTA avant 29 1015 56 AA 33 3 3 1 AKTA avant 13 12 Imjection Valve 11 10 Flow Restrictor pH Valve Column Valve UV Monitor Conductivity Monitor Inlet Valve B Quaternary Valve System Pump B
80. GE Healthcare Bio Sciences AB Bjorkgatan 30 SE 751 84 Uppsoala Sweden 2006 42 EC MD 2004 108 EC EMC 2006 95 EC LVD 1999 5 EC R amp TTE CE EC CE GE 8 AKTA avant 29 1015 56 AA EN ISO 12100 EN ISO 2006 42 EC EN IEC61010 1 UL EN 61010 1 CAN CSA 2006 95 EC C22 2 No 61010 1 EN IEC 61326 1 EN CISPR11 EMC 2004 108 EC 1 A ETSI EN 30
81. 20 95 AKTA avant 29 1015 56 AA 47 4 4 1 4 1 1 80 x 100 AKTA avant AKTA avant AKTA avant Unpacking Instructions 48 AKTA avant 29 1015 56 AA 4 4 1 4 1 2 4 1 2 AKTA avant GE
82. Iniect sample from loop Fill the loop using Manual load Y Loop type Capillary loop v ml OO Iniect sample directly onto column RE Sample inlet Fill loop vy Emptyloopwith 1 00 ml Sample volume _ml Use the same inlets as in Method Settings Inlet v InletB B1 ool Fill the system with the selected buffer AKTA avant 29 1015 56 AA 6 6 3 6 3 Column Logbook UNICORN Method Manual 1 System Control Open Method Navigator Method Navigator Er Method Navigator HX a Methods Method gt gt Folder name System Last modified Created by DoeMethod 2014 10 06 14 3 Default 3 svis 2012 11 27 14 5 Default AutoTests 2013 05 31 2013 05 31 12 0 Default Elvis1 201
83. nlet valve 8 AKTA avant 29 1015 56 AA 79 4 4 5 4 5 1 4 Process Picture System pumps Conc 8B 100 B Set9 B System flow 1 000 mlmin Setflow rate Yooo xs 0 100 wash A B Ai Start pump A wash B1 T Starpump B wash Q1 v Start Q inlet wash System wash System out w Injection valve W1 15 ml Startsystem wash 10 999 Systemflow 1 0ml min AKTAavant25 5 0ml min AKTA avant 150 Set flow rate System Pump B 5 25 30 System Pump B my 6 3 4 1 5 10 80 AKTA avant 29 1015 56 AA 4 45 45 1
84. 10 7 5 157 AKTA avant 29 1015 56 AA 143 7 7 2 Pump P9 Pump P9H O O 144 1 O oO jy Wy x1 I 0 56686700 2 20 Pump P9 P9H AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H O 3 P9H 4 20 21 4 x 1 6 0 0 Pump P9 P9H A
85. Manual instructions Pumps and pressures Pump wash BufferPro Q inlets All Manual instructions System3 Selected column type _ Select Instructions Paramelers for Pump wash Pumps and pressures Inlet A System flow Sample flow Of ey Gradient Inlet B Pump wash System wash Of _ Quaternary start concentrations BufferPro Q inlets Quaternary gradient BufferPro pH ET Column packing flow Sample inlet Flow path Monitors Rr Of Save result as Browse amp uto update of parameters during run Execute Q Quaternary Valve 2 3 Pumps and pressures System flow Flow rate 0 01 ml min 4 25 30 0 3 4 1 ml 10 ml AKTA avant 29 1015 56 AA 91 4 4 5 4 5 3 0 6
86. 28 AKTA avant 29 1015 56 AA 2 2 5 DoH9 2 5 DoH9 SJ T11364 2006 The following product pollution control information is provided according to SJ T11364 2006 Marking for Control of Pollution caused by Electronic Information Products Explanation of Pollution Control Label SJ T11363 2006
87. 5 6 pH Prepare for calibration pH 4 pH for buffer 1 1 pH pH pH 5 10 pH pH Cal Luer 6 Current value Calibrate 7 pH Cal pH 8 pH for buffer2 2 pH pH pH 9 pH 5 6 Calibrated electrode slope Asymmetry potential qt pH 7 10 Calibrated electrode slope 80 Asymmetry potential qt pH 7 60 mv Close pH Calibration pH pH AKTA avant User Manual Chapter Maintenances 110 AKTA avant 29 1015 56 AA 5
88. UPS 161 1 26 AKTA avant 29 1015 56 AA 2 2 3 3 Continue System Control Continue AKTA avant 29 1015 56 AA 27 2 2 4 2 4 AKTA avant AKTA avant
89. 5 4 7 8 2B 9 System Control End dl 106 AKTA avant 29 1015 56 AA 5 5 5 5 5 Column Valve V9 C V9H C UV Monitor U9 L 2 Conductivity Monitor C9 UV Monitor U9 L 2 MPa 20 bar 2 Conductivity Monitor C9 5 MPa 50 bar UV UV
90. 2 Conductivity Monitor C9 2 Fraction Collector F9 R 175 Ready AKTAavant mm Ready QC Pause I Continue Ready AKTA avant 29 1015 56 AA 39 3 3 1 AKTA avant UNICORN System Control 40 Wash e Pause e Hold AKTA avant 29 1015 56 AA 3 3 2 UNICORN 3 2 UNICORN UNICORN System Control System Control Administration Method Editor Evaluation UNICORN 3 2 1 UNICORN 42 3 2 2 System Control
91. 5 7 5 7 AKTA avant User Manual oh F9 R Ms gt SY UNICORN System Settings System Settings UNICORN System Control Manual System Settings AKTA avant ee Manual 111 AKTA avant 29 1015 56 AA 5 5 7 1 QuickRelease
92. 1280x1024 UNICORN Windows Windows 1 UNICORN AKTA avant 29 1015 56 AA UNICORN UNICORN 55 4 4 1 4 1 5 56 Windows 7 Aero Windows UNICORN Windows 7 High DPIl Awareness 100 UNICORN 100 AKTA avant 29 1015 56 AA 4 4 1 4 1 6 4 1 6 J J1 AKTA avant
93. 6 3 1 No 2 Yes 5 Select Columns System3 Enter or select a column individual Lode Enter ID OO Select ID O Disable column logging for this run Apply to all methods with the same column type Methods Remark Column Barcode Col Column Handling Scouting run 1 HIP umn type rep 2B6 10 Desalting Column Handling Scouting run 2 HiPrep 26210 Desaltng Column Handling Scouting run 3 HIPrep 26 10 Desalting 2 Select Columns New New Column New Column Lode EMP IC LThe Column has a uniT ag has a fixed Code and exp AKTA avant 29 1015 56 AA 125 6 6 3 3 Barcode Scanner 2 D Code Barcode Scanner 2 D ID ID Continue
94. USB pH pH pH pH pH pH 1 pH 2 3 pH 4 pH 5 6 pH pH 68 AKTA avant 29 1015 56 AA 4 2 5 4 4 2 4 2 5 AKTA avant 29 1015 56 AA 69 4 4 2 4 2 5
95. pH UV pH 136 AKTA avant 29 1015 56 AA 7 7 1 AKTA avant AKTA avant User Manual System CIP Column CIP pH pH UV O UV 7 5 157 O 7 2 Pump P9 PumpP9H 0 139 7 3 Pump P9 5S 0 8 7 4 155
96. 4 1 4 12 AKTA avant AKTA avant 66 cm AKTA avant 116 9 3 AKTA avant 29 1015 56 AA 130 51 4 4 1 4 1 3 4 1 3 AKTA avant WC E35 C 25 C 60 C 20 95 2000 2 AKTA avant 800 300 1100 52 AKTA avant 29 1015 56 AA 4 4 1 4 1 4 4 1 4
97. 2 3 4 5 1 2 W2 3 System Control 4 Process Picture Sample inlet valve 1 Buf sampleinletvalve S10 S20 33 4 So 36 3 84 AKTA avant 29 1015 56 AA 4 4 5 4 5 2 Sample Pump 5 25 30
98. 4 2 5 4 2 6 61 62 65 68 69 72 AKTA avant 29 1015 56 AA 4 4 2 4 2 1 4 2 1 AKTA avant 1IEC 60950 AKTA avant 29 1015 56 AA 61 4 4 2 4 2 2 4 2 2 J J1 62 AKTA avant AKTA avant GE Power Network UniNet 9
99. AKTA avant 29 1015 56 AA 4 4 2 4 2 2 UniNet 9 UniNet 9 UniNet 9 UniNet 9 AKTA avant AKTA avant 1 2 2 2 Power 100 240 VAC 50 60 Hz 3 AKTA avant 29 1015 56 AA 63 4 4 2 4 2 2 AKTA
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101. AKTA avant UniNet 9 UniNet 9 AKTA avant UniNet 9 AKTA ovant User Manual UniNet 9 AKTA avant 29 1015 56 AA 17 2 2 1 AKTAavant AKTA avant 20
102. 3 4 10 ml Q1 5 6 Quaternary start concentration 100 Q2 Q3 Q4 2 5 BufferPro Q1 Q2 System Pump A B Somple Pump 82 Quaternary Volve 90 Quaternary Valve Quaternary Valve Quaternary Valve System Pump A System Pump B 90 AKTA avant 29 1015 56 AA 4 4 5 4 5 3 0
103. 4 5 1 77 3 Pump P9 Ref2 6 8MPa 60 80 bar 1A 1B Pump P9H Ref 1 2 3 MPa 20 30 bar 1A 18 4 146 AKTA avant 29 1015 56 AA 7 7 2 Pump P9 Pump P9H O gt Manual instructions Flow path Column valve nsert Flow path Inlet A System Pump A Flow path Inlet B System Pump B Position Insert Pumps and Pressures Gradient Target 0 B System Pump A 100 B B PumpP9 Pumps and Pressures System flow Flow rate 5 0 ml min Insert Pump P9H Pumps and Pressures System flow Flow r
104. Execute Manual Instructions Manual instructions Instructions Alarms Alarm pre column pressure EDT Instructions Pumps Flow path Monitors Fraction collection Alarms arm system pressure p Alarm Uy A amp larm conductivity Wash settings Watch parameters dvance v Selected column type OO Disabled High alarm Low alarm Parameters for Blarm pre column pressure Mode Enabled 0 02 20 00 050 MPa 0 00 20 00 030 MPa Save result as Suto update of parameters during run Execute Mode Enabled High alarm Case Instruction execution list Browse AKTA avant 29 1015 56 AA 0 J pH 5 5 6 pH pH pH pH pH pH4 7 pH pH pH
105. 43 AKTA avant 29 1015 56 AA 41 3 3 2 UNICORN 3 2 1 UNICORN 3 2 1 UNICORN UNICORN UNICORN UNICORN 6 4 UNICORN UNICORN Administration Method Editor System Control Evaluation Administration Method Editor System Control Process Picture Evaluation Evaluation
106. 29 1015 56 AA 5 5 7 GE 3 monogram 50 250 4 Pause AKTA avant 29 1015 56 AA 115 5 Se 116 System control View Fraction Collector Content Fraction Collector Content System3 A emmm mm
107. 29 1015 56 AA 9 2011 65 EU DoHS 2012 19 EU WEEE ACPEIP RoHS 1907 2006 EC REACH AKTA avant EN IEC 61010 1 CE AKTA avant 29 1015 56 AA 1 1 4 1 4 AKTA avant AKTA avant AKTA avant AKTA avant Unpacking Instruction AKTA avant AKTA avant User Manual AKTA avant25 Product Documentation AKTA avant 150 Produ
108. EE 3 66 AKTA avant 29 1015 56 AA 4 4 2 4 2 3 AKTA avant 25 2 10 AKTA avant 150 40 AKTA avant 29 1015 56 AA 67 4 4 2 4 2 4 Barcode Scanner 2 D pH 4 2 4 Barcode Scanner 2 D pH gt gt Barcode Scanner 2 D pH Barcode Scanner 2 D
109. Remark Scouting run 2 Scouting run 3 Scouting run 1 Column Barcode 28 9288 13 12345678 0000 00 0005 Column type HiPrep 26 10 Desalting HiPrep 26 10 Desalting HiPrep 26210 Desalting SelectID Select Columns System3 Enter or select a column individual OO Enter ID Select ID Code 28 9288 13 28928813 0000 00 1234 HiPrep 261 28 9288 13 12345678 0000 00 0005 HiPrep 2610 Desalting E OO Disable column logging 28 3288 13 12345678 0000 00 0006 HiPrep Desaltng 2610 E 28 9288 13 28328813 0000 00 1234 HiPrep 2610 Desalting E Apply to all methods with the same column type Methods Column Handling Column Handling Column Handling OK Remark Scouting run 1 Scouting run 2 Scouting run 3 Column Barcode 28 9288 13 28928813 0000 00 1234 Column type HIPrep 26 10 Desalting HIPrep 26 10 Desalting HiPrep 26 10 Desalting 128 AKTA avant 29 1015 56 AA 6 4 System Control System state Run Data Run Wash Hold
110. mr 7 3 4 1 5 10 8 9 6 8 ml 10 System Pump A 8 Somple Pump 1b 82 AKTA avant 29 1015 56 AA 87 4 4 5 4 5 2 Sample Pump System Control End i 88 AKTA avant 29 1015 56 AA 4 4 5 4 5 3 0 4 5 3 9 1 Q 2 Q 3 Quaternary Valve 4 Quarternary Valve 5 Q 0 1 A1 B1 Q1 Q4
111. valve w1 15 ml Startsystem wash 10 999 System Pump A System Pump A B Sample Pump 1 Process Picture Injection valve Manual load Manual load LoopE Manual load System pump waste Inject Sample pump load Direct inject Sample pump waste 82 AKTA avant 29 1015 56 AA 4 45 4 5 1 2 3 Chromatogram PreC pressure PreCpressure AKTA avant User Manual System Control End AKTA avant 29 1015 56 AA 83 4 4 5 4 5 2 Sample Pump 4 5 2 Sample Pump SF 1
112. 1 489 3 EN ERM 1999 5 EC EMC ETSI EN 300 330 2 EN ERM SRD 9kHz 1999 5 EC 25 MHz 9kHz 30 MHz FCC FCC 15 1 2 GE FCC 15 A AKTA avant
113. 170 AKTA avant 29 1015 56 AA 9 42 42 AKTA avant 29 1015 56 AA B 77 62 58 57 sn f 173 www gelifesciences com contact GE Healthcare Bio Sciences AB Bjorkgatan 30 751 84 Uppsala Sweden www gelifesciences com avant GE GE monogram General Electric Company AKTA General Electric Company BD Becton Dickinson and Company Decon Decon Laboratories Limited Eppendorf Eppendorf AG Microsoft Windows Microsoft Corporation NUNC Thermo Scientific Thermo Fisher Scientific SARSTEDT SARSTEDT AG amp CO Seahorse Bioscience Seahorse Bioscience Inc Triton Union Carbide Chemicals and Plastic Company Inc Tween Uniqema Americas LLC VWR VWR International LLC UNICORN GE Healthcare
114. 2 11 27 14 5 Default Pi 2 Run Start Protocol 3 StartProtocol Next AKTA avant 29 1015 56 AA 123 6 6 3 4 Start Protocol Start UNICORN Select Columns Select Columns System3 Enter or select a column individual Code Enter ID OO Select ID O Disable column logging for this run DD Apply to all methods with the same column type Methods Remark Column Barcode Column type Column Handling Scouting run 2 HiPrep 26210 Desalting Column Handling Scouting run 3 HIPrep 26 10 Desalting UNICORN 124 AKTA avant 29 1015 56 AA 6
115. 33 132 pH 133 pe A 6 14 14 17 47 22 50 25 cm 73 16 26 25 171 172 66 101 49 161 101 5 11 15 5 107 133 133 23 23 10 8 Zk 77 P9 S 148 P9 P9H 139 155 157 130 15 6 UNICORN 74 95 132 44 43 130 55 23 136 20 ID 167 D 170 ID
116. 6 8 System Pump A 8 Somple Pump 82 System ee B 78 System Pump B System Pump B System PumpB 1 W1 78 AKTA avant 29 1015 56 AA 4 4 5 4 5 1 2 Process Picture njiection valve System pump waste System pump waste LoopE Loopvalve prec System pump By pass Waste rn sop Ve inj venallond Gr ppv Inject Sample pumpload Direct inject Sample pump waste SY P 3 Process Picture
117. AKTA avant 1 OI 1 1 1 2 i 13 ee 1 4 wwawaaawawaawaaaswen 2 1 2 2 23 2 4 ne 2 5 DoHS ce ne 3 1 AKTA avant 0 3 2 UNICORN 0 321 UNICORN ee 3 2 2 System Control ssessssssssssssssssssssssssssssssssssssssssssss 4 1 i 12 4 1 3 4 1 4 4 1 5 4 1 6 4 2 1 ee 42 2 4 2 3 4 2 4 Barcode Scanner 2 D pH 4 2 5 4 2 6 4 3 4 4 UNICORN 4 5 cc 45 1 4 5 2 Sample Pump 4 5 3 0 4 6 ne ee 5 1 5 2 Oo On Cn 28 69 AKTA avant 29 1015 56 AA 5 3
118. KTA avant 29 1015 56 AA 145 7 7 2 Pump P9 Pump P9H O 140 1 2 20 3 4 T20 5 An 20 6 7 8 Pump P9 P9H 20 System PumpA AL1 System PumpB B1 Sample Pump S1 2
119. N System Control Pause System Control Fie Edit Wew ee Svstem Tools Help eol oo 2 Power oO AKTA avant 29 1015 56 AA 25 2 2 3 AKTA avant UNICORN UNICORN Not connected 10 UNICORN UNICORN UPS UPS AKTA avant
120. ate 25 0 ml min Insert Execute Manual instructions Flow path Column valve nsert Flow path Sample inlet Position Insert Flow path Injection valve Position Direct inject Insert Pumps and Pressures Sample flow Flow rate 25 0 ml min Insert Execute 6 2 f AKTA avant 29 1015 56 AA 147 7 7 3 Pump P9 S O 7 3 Pump P9 S O Pump P9 S 0 0
121. ct Documentation 1 1 UNICORN UNICORN Help UNICORN Fl UNICORN Online Help and Documentation UNICORN Help UNICORN Help Getting started with Evaluation Evaluation UNICORN 7 0 Evdluation UNICORN Method Manual UNICORN AKTA avant 29 1015 56 AA 11 1 1 4 UNICORN Administration and Technical Manuall UNICORN UNICORN UNICORN Evaluation Manual UNICORN Evaluation Classic UNICORN UNICORN System Control UNICORN Manualt 1 UNICORN 12 AKTA avant 29 1015 56 AA 2
122. ed EFUP shall be done in accordance with the Product Maintenance Procedures This product must not be disposed of as unsorted municipal waste and must be collected separately and handled properly after decommissioning AKTA avant 29 1015 56 AA 29 2 2 5 DoH9 Name and Concentration of Hazardous Substances Table of Hazardous Substances Name and Concentration 28 9308 42 O O O O 28 9763 37 O O O O 29 0113 60 O O O O 29 0113 61 O O O O 29 0113 62 O O O O 29 0906 89 O O O O 29 0906 91 O O O O 29 0113 53 O O O O 29 0113 58 O O O O 0 SJ T11363 2006 K SJ T11363 2006 0 SJ T11363 2006
123. ion Valve V9 Inj V9H Inj W1 We pH Valve V9 pH V9H pH e W3 Outlet Valve V9 O V9H O es W AKTA avant 29 1015 56 AA 5 5 2 4 2 3 65 Frac AKTA avant User Manual AKTA avant 29 1015 56 AA 101 5 5 3 5 3
124. ivity Monitor Built in fraction collector AKTA avant 29 1015 56 AA 35 3 3 1 AKTA avant AKTA avant 25 AKTA avant 150 2 Inlet Valve A 2 Inlet Valve B Inlet Valve X1 Inlet Valve X2 Sample Inlet Valve Versatile Valve Loop Valve Column Valve 2 Outlet Valve Outlet Valve External Air Sensor L9 1 5 External Air Sensor L9 1 2 O box UV Monitor 2 Conductivity Monitor Fraction Collector Quaternary Valve Q9 System Pump A P9A P9H A System PumpB P9B P9H B 36 AKTA avant 29 1015 56 AA Sample Pump P9 S P9H Pressure Monitor R9 3 3 1 AKTA avant System Pump A System Pump B
125. jection UV2nd Run Log PreC pressure DeltaC pressure Manual load gt 0 22 MPs cons 0 00 ms cem ph Off je 2 00 V1 mAU mm Al Hm A EWR ee TAT Waste eC P mm M ESyP F oo Ee UV nd mmm Restrictor mm Frac 3 0 01 MPs 0 High m 20 00 a Buffer S py A Available components without a fbred place 43 825 maAU UV 2nd IO box Manual Run Block No watch Connection Conn in control Controlled by Default HCE C8ZH05J View Run Log Run Log AKTA avant 29 1015 56 AA 43 3 3 2 UNICORN 3 2 2 System Control Open Method Navigator Method Navigator Run Hold Pause Continue End 44 Customize Customize
126. thentication User Name Defautt Domain Access ToEverything Group Start VY Administration System Control Method Editor Evaluation ox Gance Optons lt OK UNICORN 74 AKTA avant 29 1015 56 AA 4 4 4 UNICORN UNICORN 1 System Control Connect to Systems System name Control View LL Systeml System2 System4 System5 System6 System 2 Connect to Systems Control OK UNICORN AKTA avant User Manual AKTA avant 29 1015 56 AA 75 4 4 5 Je 4 2 76
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