EN ENGLISH User's Guide
Contents
1. 7 1 ENGLISH User s Guide t l chargeable sur www gilson com auf www gilson com herunterladen 4 GILSON PIPETMAN 72eo Contents Page 1 INTRODUCTION 2 2 PARTS CHECK LIST 3 3 DESCRIPTION 3 4 THE VOLUME 4 5 5 6 GENERAL GUIDELINES FOR GOOD PIPETTING 10 7 ACCESSORIES 11 8 GLP FEATURES 12 9 TROUBLESHOOTING 12 10 LEAKTEST 14 11 MAINTENANCE 15 12 CLEANING AND DECONTAMINATION 19 13 SPECIFICATIONS 23 14 SPARE PARTS 25 EC DECLARATION OF CONFORMITY 29 1 INTRODUCTION PIPETMAN Neo is a fully adjustable air displacement pipette and is used with disposable tips PIPETMAN Neo is an evolution of the classic PIPETMAN P to address the growing susceptibility of pipette users to repetitive strain injuries The implementation of new features toimprove pipetting comfort has no impact on the legendary robustness accuracy and precision of PIPETMAN P Six single channel models cover a volume range from 0 2 uL to 1000 uL gt Two 8 and two 12 multichannel models cover a volume range from 2 uL to 200 uL 2 PARTS CHECK LIST Justtake a momentto verify thatthefollowing items are present PIPETMAN Neo User s Guide Safety bag Certificate of conformity including bar code sticker 3 DESCRIPTION IS Please refer to the following chapters for a full description of the different parts and functions of the pipette Color coded push b2. Unscrew the con necting nut turning counter clockwise Press push button to extract the head To autoclave Clean the parts to be autoclaved especially the tip holders and the ejector spacer Put the lower part in an autoclaving sack Autoclave for 20 minutes at 121 C 0 1 MPa o Check that the autoclaved part is dry before re assembling the pipette Set the pipette aside to stabilize at room temperature 21 PIPETMAN 72eo To fit the lower part on the body Press on the push button Insertthelower part into the ejection rod Release the push button Screw the connect ing nut turning clockwise o Press on the tip ejector button Insert the ejector clip and release the tip ejector button Chemical Decontamination You may choose to decontaminate your pipette chemically in accordance with your own procedures Whatever decontaminant you use check with the supplier of the decontaminant that it is compatible with stainless steel and the plastics used in the construction of the pipette PA Polyamide PBT Polybutylene Terephtalate PC Polycarbonate PC PBT Polycarbonate PolybutyleneTerephtalate POM Polyoxymethylene or PVDF Polyvinylidene Fluoride Upper Part handle Wipe the upper part handle of the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant e Wipe the upper part of the pipette with a soft c
3. tip ejector with a soft cloth or lint free tissue impregnated with soap solution Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solution to remove all dirty marks If the pipette is very dirty a brush with soft plastic bristles may be used o Wipe the entire pipette and the tip ejector with a soft cloth or lint free tissue soaked with distilled water Refit the tip ejector and allow the pipette to dry Internal The following components only can be immersed in a cleaning solution connecting nut tip ejector tip holder piston assembly seal and O ring 1 Disassemble the pipette as described in the Chapter 11 Maintenance amp Set aside the upper part in a clean dry place PIPETMAN 72eo Clean the individual components of the lower part of the pipette using an ultrasonic bath 20 minutes at 50 C or with a soft cloth and brushes Small round brushes with soft plasticbristles may be used to clean the interior of the tip holder 4 Rinse the individual components with distilled water 9 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue Q Reassemble the pipette as described in the Chapter 11 Maintenance Multichannel models The following components only can be immersed in a cleaning solution tip ejector ejector locks and ejector spacer CD Remove the tip ejector and the ejector spacer e Immersethetip
4. 200 F 14403 in 0 50 0 25 SE lt 12 and t10 lt 0 40 E 5 12 12 200 14404 2 00 lt 0 60 07 lt 12 L The data given in the tables conform to the ISO 8655 2 Standard 24 14 SPARE PARTS Single channel models Service Kit 1st level includes 3piston seals 3 O rings 1tip holder 3 Service Kit 2nd level includes 1 push button ON 1 connecting nut 1tip ejector or only for the P2N and P10N models 1tip ejector and 1 adapter 25 PIPETMAN 77eo P2N F144561 and P10N F144562 Description P2N P10N C D E Service Kit 1st level F144501 F144502 A B F1 F2 Service Kit 2nd level F161970 F161971 Seal 0 ring 5 sets F144861 F144862 F2 Tip ejector Adapter F144879 F144879 P20N F 144563 and 100 F144564 Description P20N P100N Service Kit 1st level F144495 F144496 Service Kit 2nd level F161972 F161973 Seal 0 ring 5 sets F144863 F144864 P200N F144565 and P1000N F 144566 Description P200N P1000N Service Kit 1st level F144497 F144498 Service Kit 2nd level F161974 F161978 Seal 0 ring 5 sets F144865 F144866 8x20 F 14401 and 12x20 F14402 Description 8x20 12x20 Tip ejector F507005 F507006 Ejector lock F507008 F507008 Ejector spacer F507001 F507003 Description 8x20 12x20 Tip ejector F507005 F507006 Ejector lock F507008 F507008 Ejector spacer F507001 F507003 26 Multichannel models Ejector spacer 8 channels Eject
5. ble COLORIS clips mixed colors set of 10 F161301 COLORIS clips red set of 10 F161302 COLORIS clips yellow set of 10 F161303 COLORIS clips blue set of 10 F161305 COLORIS dips green set of 10 F161304 COLORIS clips white set of 10 F161306 PIPETMAN 72eo WithTHEJIMMY handsfree microtube opener you can open both snap cap and screw cap microtubes THE JIMMY set of 3 F144983 To protect the piston when pipetting corrosive liquids you can use a specific tip holder and filter for the model P1000N Corrosion protection kit tip holder a bag of 10 filters F144570 8 GLP FEATURES The Serial Number is engraved onthe body of the pipette It provides unique identification of your pipette and the date of manufacture Ex CG 12326 The Bar Code on the box and the certificate of conformity provide traceability of your pipette 9 TROUBLESHOOTING A quick inspection of the pipette may help you to detect a problem IS You may download from the Gilson website www gilson com the 2 minute inspection which shows how to perform a quick diagnosis of your pipette Before returning any pipette ensure that it is completely free of chemical biological or radioactive contamina tion Referto Chapter 12 Cleaningand Decontamination After decontamina tion use the safety bag provided with the pipette to return it to your local Gilson authorized Service C
6. de of the tip using a medical wipe however if you do sotake care to avoid touching the tip s orifice Second stop Dispense Place the end of the tip against the inside wall of the recipient vessel at an angle of 10 to 40 PIPETMAN 72eo e Press the push button slowly and smoothly to the first stop amp Wait for at least a second then press the push button to the second stop to expel any residual liquidfromthetip Keepthe push button pressed fully down and whileremovingthe pipette draw the tip along the inside surface of the vessel o Release the push button smoothly Eject the tip by pressing firmly on the tip ejector button 6 GENERAL GUIDELINES FOR GOOD PIPETTING CD Makesurethatyou Table Immersion Depth operate the push and Wait Time button slowly and Immersion Wait Time Depth mm seconds smoothly When aspirating keep the tip ata 70 constant depth N P200N below the surface P1000N of the liquid refer 8x20 12x20 to the table 8x200 12200 e Changethetip before aspirating a differentliquid sample or reagent o Change thetip if a droplet remains at the end of the tip from the previous pipetting operation Each newtip should bepre rinsed with the liquid to be pipetted Q Liquid should never enter the tip holder to prevent this press and release the push button slowly and smoothly never turn the pipette upside down neve
7. e use of PIPETMAN DIAMOND Tips with your PIPETMAN Neo is strongly recommended TheseTips madefrom pure polypropylene have the Gilson logo engraved on their collar ensuring that you have a genuine Gilson product Plastic tips are fora single application they must not be cleaned for reuse PIPETMAN Neo can also be used with the main tip brands PIPETMAN 72eo Fitting the tips Single channel models To fit a new PIPETMAN DIAMOND Tip push the tip holder into the tip using a slight twisting motion to ensure a firm airtight seal IS For the P2N and PION models a dual position adapter plastic is required to fit DL10 tips long tips or D10 tips short tips The metallic rod of the tip ejector is shaped so that the adapter may be clipped to it in either of two positions P2Nand P10N modelsare delivered with the adapter in place positioned in the longer slot ready to use DL10 tips When D10 tips which are shorter are used the adapter must be repositioned in the shorter slot as follows CD Pull the adapter down from the metallic rod amp Turn the adapter through 180 C Refit the adapter so that the end of the metallic rod engages the shorter slot of the adapter Finally check that the dimple on the metallic rod is engaged in the corresponding hole on the adapter Dual position adapter for P2N and P10N with DL10 Multichannel models Ejector spacer PIPETMAN DIAMOND Tip compatib
8. eal 0 ring gt lt gt Tip holder gt Changing the Tip holder no tools required CD Remove the tip ejector see above Unscrewtheconnectingnutbyturningitcounter clockwise Carefully separate the lower and upper parts 16 4 Remove the piston assembly O ring and seal Clean autoclave or replace the tip holder Qo Reassemblethe pipette refer to the figure p 16 TA Tighten the connecting nut turn clockwise Refit the tip ejector see above Servicing the Piston Assembly You may remove the piston assembly for cleaning purposes only Ifthe piston assembly is changed the pipette must be adjusted and calibrated in a Gilson authorized Service Center The piston assembly must not be autoclaved Remove the tip ejector see above Unscrewtheconnecting nut by turningit counter clockwise Carefully separate the lower and upper parts 2 Remove the piston assembly O ring and seal Clean and decontaminate the piston assembly Q Reassemblethe pipette refer to the figure p 16 GO Tighten the connecting nut turn clockwise Refit the tip ejector see above Changing the O ring The O ring and seal are on the piston they must not be autoclaved if worn or damaged in any way chemical or mechanical they must be replaced The dimensions of the O ring vary depending on the model of pipette D Remove the tip ejector s
9. ee above amp Unscrew the connecting nutbyturningitcounter clockwise Carefully separate the lower and upper parts o Remove the piston assembly O ring and seal 17 PIPETMAN 72eo Clean or replace the seal then the O ring O Reassemble the pipette refertothefigure p 16 D Tighten the connecting nut turn clockwise Refit the tip ejector see above Multichannel models Changing the Tip ejector CD To remove the tip ejector keep both ejector locks depressed 2 Pull the tip ejector down e To refit the tip ejector gently re insert the tip ejector vertically into the rails of the ejector support Pull lightly on the tip ejector to check the position Ejector lock 12 CLEANING AND DECONTAMINATION PIPETMAN Neojis designed so thatthe parts normally contact with liquid contaminants can easily be cleaned and decontaminated However because the models P2N and P10N contain miniaturized parts itis best not to disassemble these pipettes yourself please contact your local Gilson authorized Service Center I Youmayrefertothedecontamination procedure available on the Gilson website www gilson com Liquid must never enter the upper part handle of any pipette Cleaning The pipette must be cleaned as described below before itis decontaminated Soap solution is recommended for cleaning PIPETMAN Neo Single channel models External CD Remove the tip ejector e Wipe the
10. ejector ejectorlocks and ejector spacerinthecleaning solution or wipethem with a soft cloth or lint free tissue impregnated with the cleaning solution Rinse the components with distilled water Wipe the entire pipette with a soft cloth or lint free tissue impregnated with the cleaning solution 5 Wipe it with distilled water Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue Refit the tip ejector as described in page 18 Changing the tip ejector Autoclaving Single channel models The upper part body and the piston assembly of the pipette are not autoclavable Only the following parts may be autoclaved tip ejector tip holder and connecting nut The O ring and seal are not autoclavable they may be cleaned or replaced with the one specified in Chapter 14 Spare Parts 20 Clean the parts to be autoclaved especially the tip holder Put the parts in an autoclaving sack Autoclave for 20 minutes at 121 C 0 1 MPa a Checkthatthe parts are dry before re assembling the pipette Set the pipette aside to stabilize at room temperature Multichannel models The complete lower part can be autoclaved 20 minutes at 121 C 0 1 MPa The lower part has to be removed from the body Toremove thelowerpart from the body Keep the tip ejector button depressed Turn the ejector clip counter clockwise and separate it from the handle
11. enter The following table may help you to identify and correct the problem you might encounter Symptom Pipette is leaking sample Pipette won t aspirate Pipette is inaccurate Pipette is not precise Possible Cause Damaged tip holder Worn O ring or seal Worn O ring Damaged tip holder Connecting nut is loose Damaged or corroded piston Improper repair or assembly Improper repair or assembly Unscrewed tip holder Connecting nut is loose Tip holder is loose Connecting nut is loose Incorrect operator technique Damaged or corroded piston s Damaged tip holder s Worn O ring or seal Low quality tips Damaged tip holder s Damaged tip ejector Ejector spacer is missing The ejector spacer is damaged The tip ejector is loose The ejector lock is misaligned Not applicable for multichannel models Action Replace the tip holder Replace both parts Replace both parts Replace the tip holder Tighten connecting nut Return pipette to supplier See Chapter 11 Maintenance See Chapter 11 Maintenance Tighten connecting nut Tighten connecting nut Tighten connecting nut Tighten connecting nut Operator training Return pipette to supplier Replace the tip holder Replace both parts Use PIPETMAN DIAMOND tips Replace the tip holder Replace tip ejector Mount the spacer on the tip ejector Replace the ejector spacer Assemble the tip ejector properly Align the ejector lock How
12. ever if you can t solve the problem contact your Gilson representative PIPETMAN 72eo 10 LEAK TEST Thistest may be performed at any timeto checkthat the pipette does not leak especially after perform ing a maintenance or decontamination procedure If a pipette fails this test replace the O ring and seal After making sure that the pipette is correctly reassembled repeat this test not applicable to multichannel pipettes For the P2N to P200N 8x20 12x20 8x200 and 12x200 models CD Fit a PIPETMAN DIAMOND Tip Set the pipette to the maximum volume given in the specifications and pre rinse Aspirate the set volume from a beaker of distilled water o Maintain the pipette in the vertical position and wait for 20 seconds If water droplet appears at the end of the tip there is a leak If you see no droplet re immerse the tip below the surface of water 7 The water level inside the tip should remain constant if the level goes down there is a leak For the P1000N model CD Fit a PIPETMAN DIAMOND tip Set the pipette to the maximum volume given in the specifications Aspirate the setvolumefrom a beakerofdistilled water Maintain the pipette in the vertical position and wait for 20 seconds O If a water droplet appears at the end of the tip there is a leak 11 MAINTENANCE Routine maintenance will help keep your pipette in good condition ensuring a continued h
13. igh level of performance Maintenance is limited to cleaning or autoclaving the parts specified under Chapter 12 Cleaning and Decontamination or to replacing the push button connecting nut tip ejector tip holder seal and O ring PIPETMAN Neo P2N and P10N should not be disassembled so you may only replace the push button tip ejector dual position tip ejector and its adapter With these pipettes if the tip holder is damaged the piston may also be damaged For multichannel models the lower part should not be disassembled only the push button the connecting nut and the tip ejector can be replaced After replacing any parts you should verify the performance of your pipette following the verification procedure available on the Gilson website www gilson com If the pipette needs to be readjusted please contact your local Gilson authorized Service Center Single channel models Changing the Tip ejector D To remove the tip ejector keep the tip ejector button depressed and pull down on the flanged upper part of the tip ejector with the other hand Torefitthetip ejector keepthetip ejector button depressed slide the end of the tip ejector over the end of the tip holder and push the plastic end of the tip ejector back into the body of the pipette until it is gripped firmly by the metal tip ejector rod PIPETMAN 72eo Color coded push button lt Connecting nut Piston assembly S
14. ility 8x20 12x20 DL10 DFL10 DF30 D200 8x200 12x200 D200 D300 DF100 DF200 DF300 PIPETMAN 72eo PIPETMAN DIAMOND Tip Tipack and Towerpack Rocky Rack PIPETMAN DIAMOND Tips are best fitted from the patented Rocky Rack available only in our Tipacks and Tower packs Rocky Rack is the dome shaped part of the pack that contains the tips Rocky Rack makes it easy to securely fit the tips to a multichannel pipette ensuring an airtight seal on all channels without the need to use undue pressure or to touch the tips Pre rinse the tips Some liquids e g protein containing solutions and organic solvents can leave a film of liquid on the inside wall of the tip pre rinse the tip to minimize any errors that may be related to this phenomenon Pre rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel or to waste Subsequent volumes that you pipette will have levels of accuracy and precision within specifications Aspirate amp D Press the push button to the first stop this corresponds to the set volume of liquid amp Holdthepipetteverticallyandimmersethetipin the liquid see immersion depth table page 10 Releasethe push button slowly and smoothly to top position to aspirate the set volume of liquid Wait one second time depends on model see table then withdraw the pipette tip from the liquid You may wipe any droplets away from the outsi
15. loth orlint free tissue soaked with distilled water or sterile water 22 Lower Part Volumetric module Single channel The following components only can be immersed in a decontaminant solution connecting nut tip ejector tip holder piston assembly seal and O ring Multichannel The following components only can be immersed in a cleaning solution tip ejector ejector locks and ejector spacer Remove the tip ejector and the ejector spacer Immersethetip ejector ejector locks and ejector spacerinthecleaning solution or wipe them with a soft cloth or lint free tissue impregnated with the cleaning solution e Rinse the components with distilled water 4 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with the cleaning solution Wipe it with distilled water Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 0 Refit the tip ejector as described page 18 Changing the tip ejector 13 SPECIFICATIONS PIPETMAN Neo is a high quality pipette that offers excellent accuracy and precision Thefigures given in the Gilson Maximum Permissible Errors table page 24 were obtained using PIPETMAN DIAMOND Tips These figures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used Each pipette is inspected and validated by qualified technicians in accordance with the Gilson Quality System Gilson declares that its manufactured pi
16. or spacer 12 channels 27 idi IPETMAN 77eo EC DECLARATION OF CONFORMITY The company GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 www gilson com Hereby certifies on its sole responsibility that the products listed below Pipetman Neo Single channel P2N P10N P20N P100N P200N P1000N Pipetman Neo Multichannel 8x20 12x20 8x200 12x200 comply with the requirements of the following European Directives 98 79 EC on In Vitro Diagnostic Medical Devices Annex Ill self declared Villiers le Bel May 3 2010 A El Sayed H Ledorze General Manager Quality Manager seusov 29 PIPETMAN 77e NOTES NOTES LT801521 B 2010 Gilson SAS All rights reserved May 2010 English Printed in France Specifications subject to change without notifications errors omitted www gilson com sales gilson com service gilson com training gilson com FSC MIX Paper FSC 012152 Gilson Inc World Headquarters 3000 Parmenter Street PO Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France Tel 33 1 34 29 50 00 Fax 33 1 34 29 50 20 GILSON
17. pettes comply with the requirements of the ISO 8655 standard by type testing 23 PIPETMAN 77eo The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 Gilson Maximum Permissible Errors Single channel Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference uL Systematic Random Systematic Random or uL error uL error uL error uL x 0 012 0 08 lt 0 04 P2N p a x 0 012 0 08 lt 0 04 144561 lt 0 014 0 08 lt 0 04 x 0 012 E02 lt 0 08 P10N lt 0 030 0 12 0 08 F144562 lt 0 040 0112 0 08 lt 0 03 0 20 lt 0 10 P20N 0 lt 0 05 0 20 lt 0 10 F144563 0 lt 0 06 0 20 lt 0 10 lt 0 10 lt 0 30 P100N i lt lt lt 030 144564 x 0 15 au lt 030 lt 0 20 l lt 0 60 P200N lt 0 25 lt 0 60 F144565 lt 030 l lt 0 60 lt 0 6 P1000N lt 10 F144566 lt 15 lt 30 5 30 lt 30 It IRR co co co NS Witha precise pipetting technique see Chapter 6 General guide lines for good pipetting the P2N model may be used to aspirate volumes as low as 0 1 uL and the PION model as low as 0 5 uL Multichannel Maximum Permissible Errors Model Volume Gilson 150 8655 Reference uL Systematic Random Systematic Random error uL error uL error uL error uL 8x20 F14401 in 0 10 lt 0 08 0 40 lt 0 20 and 0 20 xz 0 10 0 40 lt 0 20 12x20 F14402 0 40 lt 0 20 0 40 lt 0 20 8x
18. r lay the pipette on its side when there is liquid in the tip Ir you use the same tip with a higher volume pre rinse the tip For volatile solvents you should saturate the air cushion of your pipette by aspirating and dispensing the solvent repeatedly before aspirating the sample 9 When pipetting liquids with temperatures different to the ambient temperature pre rinse the tip several times before use 0 You may remove the tip ejector see Chapter 11 Maintenance to aspirate from very narrow tubes ap After pipetting acids or other corrosive liquids that emit vapors remove the tip holder rinse the piston O ring and seal with distilled water For the model P1000N by using a specific tip holder equipped witha filter you can increase the lifetime ofthe piston see Chapter 7 Accessories qo Do not pipette liquids having temperatures above70 Corbelow 4 C The pipette can be used between 4 Cand 40 C but the specifications may vary according to the temperature refer to the ISO 8655 2 standard for conditions of use 7 ACCESSORIES To make pipetting more comfortable and more secure Gilson has developed several accessories Ch To avoid the possibility of liquid running backinto the pipette store the pipette vertically CARROUSEL Pipette stand 7 pipettes F161401 TRIO stand 3 pipettes F161405 SINGLE pipette holder F161406 Toidentify or personalize your pipette COLORIS clips are availa
19. utton Thumbwheel Tip ejector button Connecting nut Tip ejector CE PIPETMAN DIAMOND tip PIPETMAN 72eo Color coded gt push button Thumbwheel Tip ejector button Handle Connecting nut Ejector clip Ejector lock Tip holders EL ev support x Cover Tip ejector Ejector spacer E PIPETMAN DIAMOND tips 4 SETTING THE VOLUME The volume of liquid to be aspirated is set using the volumeter The dials are colored either black orred to indicate the posi 7 5 pL tion of the decimal point depending on the model see examples P200N 125 uL Color of volumeter numbers Black Red Increment P2N 0 01 uL 0 002 uL PION to P20N 0 1 uL 0 02 ul P100N P200N 0 2 uL P1000N ml 0 002 mL The volume is set by Push button Thumbwheeel turning the thumbwheel or the push button The push button makes it easier and quicker to set volumes especially when wearing gloves The thumbwheel may be turned using only one hand to slowly reach the required setting To obtain maximum accuracy when setting the volume proceed as follows gt when decreasing the volume setting slowly reach the required setting making sure not to overshoot the mark gt when increasing the volume setting pass the required value by 1 3 of a turn and then slowly decrease to reach the volume making sure not to overshoot the mark 5 PIPETTING For optimum performanc
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