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VIAFLO II Electronic Pipettes Operating instructions

1. n iw Pret Insert the tips into the liquid to be transferred Press Repeat Dispense laesae soul and release the Run Key 7 to aspirate the volume PR selected in the first step of your protocol shown on Manual Pipet he Run screen Reverse Pipet the Run screen HELP pace To execute subsequent steps press the Run Key For a detailed description see 5 2 Detailed description of pipetting modes on page 29 You can change the parameters of your pipetting mode at any time see the following sections 4 3 2 Blowout modes During the last dispense of a program a blowout is performed automatically Liquid may be aspirated back into the tips when the pistons move back to the home position a process called blowin There are two ways to perform the blowin Automatic blowout Pressing and releasing the Run Key starts the dispense with blowout and automatic blowin You can choose a timed delay between the blowout and the blowin see Pipetting Delayed blowin under 3 4 1 Preferences on page 16 Two step blowout Perform a two step blowout to manually delay the blowin Press and hold the Run Key to start dispense with blowout Remove the tips from the target vessel Release the Run Key to start blowin www integra biosciences com 21 VIAFLO II Pipettes Operating instructions VOO Operation 4 3 3 Recommendations for pipetting INTEGRA Biosciences recommends the following techniques for enhan
2. Repeat Dispense Allows dispensing multiple aliquots of the same volume without refilling the tips after each dispense for fast microplate filling and processing Sample Dilute Allows aspirating of sample and diluent divided by a defined air gap into one tip followed by a complete dispense Pipet Mix Allows multiple mixing by aspiration and dispensing of defined volume for automatic re suspension of pellets Manual Pipet Allows the operator to control the aspiration and dispensing up to the set volume Reverse Pipet Allows liquid transfers of viscous or high vapor pressure liquids by preventing introduction of any air into the sample The aspiration volume is higher than the volume to be dispensed Variable Dispense Allows dispensing multiple aliquots of different volumes Variable Aspirate Allows aspirating multiple aliquots of different volumes Sample Dilute Mix Allows aspirating two liquids separated by an air gap followed by a complete dispense and Mix step Serial Dilution Allows aspirating a transfer volume followed by a mix Rows and Mix Cycles are tracked on the display Custom Allows to create and store of up to 20 multi stepped pipetting protocols Press the OK to access the pipetting mode and to start defining parameters 28 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 Detailed description of pipe
3. NOTE Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting To dispense liquids with low viscosity and high vapor pressure such as ethanol use relative fast pipetting speeds and avoid prolonged pauses for aspiration 24 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Operation 4 4 4 Pace The Pace option sets the time gap between dispenses in repeat pipetting Pace is used in the Repeat Dispense and Variable Dispense modes While you press and hold the Run Key the pipette will dispense multiple programmed volumes with the selected pace Release the Run Key to stop the paced dispense Press Run to continue dispensing Repeat Dispense mam RepeatDisnense mJ Use the Touch Wheel to select the desired Pace Press RUN to go option and press OK bAspirate 250 0pl Select the pace from None 1 slowest to 9 fastest zl re Press OK to save your setting 4 4 5 Count Mix Cycle and Rows The Count Mix Cycle and Rows steps are used in various modes see 5 2 Detailed description of pipetting modes on page 29 Each is accessed with the Edit option Use the Touch Wheel to highlight the step and press OK Count sets the number of dispensing steps Mix Cycle sets the number of mixes In serial dilution mode rows sets the number of columns A column indicator will notify the number of dilutions performed Columns first number and M
4. 6 4 6 Obtaining the Actual Volume INTEGRA certifies the pipettes at 10 50 and 100 of the nominal value For each volume 5 measurements are taken from all channels General 1 2 3 Always use new unused GripTips also when changing the test volume e g from 125 ul to 12 5 pl Always pre wet section 6 4 4 when using a new GripTip For a low volume measurement first select the maximum volume for the pre wet and then change to the volume to be measured After dispensing perform a touch off to make sure no liquid remains on the tip Gravimetric testing 1 2 3 Write down the ambient temperature and air pressure Perform a pre wet see 6 4 4 In the Pipet mode program the high test volume and set pipette to dispense at speed 8 for 125 ul and 300 ul and speed 6 for 12 5 ul 1250 ul and 5000 ul The first and second dispense to the balance should not be recorded After each dispense re tare the balance Aspirate the Target volume of water keeping the pipette in a vertical to 30 degree position while immersing the pipet tip 2 3 mm below the surface of the water When withdrawing the tip from the liquid gently wipe the tip against the side wall of the vessel to remove any liquid from the outside of the pipet tip Start the first measurement Always pipet directly into the liquid of the weigh container on the balance During dispense keep the run button pressed until the pipette is removed again from the weigh v
5. Operating instructions VOO Maintenance 9 Screw counterclockwise to attach the Cylinder Assembly to the body of the pipette Check the pipette performance after reassembly 6 1 2 Assembly of multichannel pipettes If necessary disassembly the lower portion of multichannel pipettes before sterilization Do not open the housing of the lower portion a Step 1 Disassembly Rotate counter clockwise to remove the lower housing Reassembly Rotate clockwise until a stop is felt then back off to align both volume labels Step 2 Disassembly Gently pull to separate the upper and lower housing to expose the ball and socket Disassembly Disengage the ball and socket to separate the parts l Step 3 Reassembly Reengage the ball and socket to reconnect Le Reassembly e Gently push together the upper and lower assembly 6 2 Sterilization If the surface of the VIAFLO II Electronic Pipettes have been in contact with biohazardous material they must be decontaminated in accordance to good laboratory practice Wipe the clean surface with a lint free cloth lightly soaked e g with the following disinfectants Ethanol 70 Microcide SQ 1 64 Glutaraldehyde solution 496 Virkon solution 1 396 Follow the instructions provided with the reagents It is not recommended to autoclave the VIAFLO II pipettes except for decontamination before sending them for service and repair Only the lower assembly of the V
6. Charging the battery 14 8 2 1 Charging the battery on a stand 14 3 2 2 Charging the battery with Universal Power Supply 15 3 3 Exchanging the battery 15 3 4 Toolbox adapt your VIAFLO II Electronic Pipettes 15 9 4 1 Preferences iu ced etta hee oes et 16 3 4 2 Calibration amp Services 17 3 4 3 Communications 00 2 eee eect saaara aa iuan 18 3 4 4 Device Information 19 8 45 Language reins run tenes es wan cet disent 19 8 4 6 Write protect front tn un enden rat 19 www integra biosciences com 3 VIAFLO II Pipettes Operating instructions VOO Chapter 4 Chapter 5 Operation 4 1 Turmron ofrthie device e reote ra tes 20 4 2 Attaching and removing GripTip pipette tips 20 4 3 Startpipettingi cui rtr eo ie et teh ecd ete tes 21 4 33 PIpettingusie o eter ed lente 21 4 3 2 BIOWOULTIOGOS iii nahe tei eee 21 4 3 3 Recommendations for pipetting 22 4 4 Pipetting options and settings sse 23 4 4 Editoptlon nean x toe te etse 23 4 4 2 Volume selection 23 4 4 3 Speed selection 24 AAA Pa6Q ico oce eri EE T E E 25 4 4 5 Count Mix Cycle and RoWwS 25 AAG Help eere eee vite dere 25 4 5 Troubleshooting FAQ ss 26 4 51 Generali ee eee cede 26 4 5 2 ElectrOnlousei mitt e td to et betae ders 27 Pipetting modes 5 1 O
7. for measurement control and laboratory use EMC requirements Standards for Canada and USA CAN CSA C22 2 Safety requirements for electrical equipment for measurement No 61010 1 control and laboratory use General requirements UL Std No 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements FCC Part 15 Class A Emission Zizers September 9 2013 BS luhe TF Me Elmar Morscher Thomas Neher CEO Quality Manager VIAFLO II Pipettes Operating instructions VOO Table of Contents Chapter 1 Chapter 2 Chapter 3 Introduction Tat Symbols used etu entente te mer 7 1 2 Int ndediuS x is siemens e UR ee need aen ee 7 1 93 Safety NOES 35 ah Itb c eee OE kes 8 Description of the device 2 1 Scope of delivery ete ne e deo 9 2 2 Pipette configurations 9 2 3 Overview of the VIAFLO II Electronic Pipettes 10 2 3 1 VIAFLO II pipette parts 20 0 cece eeeeeeseeteeeeseeteaeeeaeeeneeees 10 2 912 Back VIEW sr ie eg e AA 11 2 9 9 Display eom ake alae reir 11 2 94 Touch whaeel mee ede 12 2 3 5 Left and right arrow buttons eese 12 2 9 6 Purge button unes nan 12 2 94 Run keys st endi em ineeueiiieonat 12 2 3 8 TIp eJector ska tio pe eU rec leto ect 13 2 9 9 Resetbutton o d pev oteietee ridens 13 Installation 3 1 Operating environment 14 3 2
8. ments 96 S96 496 S96 ul ul pl 1 4011 0 5 12 5 0 01 1 25 5 00 4 00 16 00 8 00 6 25 1 50 0 80 3 20 1 60 12 5 1 00 0 40 1 60 0 80 1 4012 5 0 0 1 12 5 3 00 1 00 12 80 4 80 125 0 62 5 1 20 0 40 2 56 0 96 125 0 60 0 20 1 28 0 48 1 4013 10 0 0 5 30 2 00 0 60 13 33 5 00 300 0 150 1 00 0 30 2 67 1 00 300 0 60 0 15 1 33 0 50 1 4014 50 0 1 125 3 00 0 60 12 80 4 80 1250 0 625 1 00 0 30 2 56 0 96 1250 0 60 0 17 1 28 0 48 1 4015 100 0 5 500 3 00 0 75 8 00 3 00 5000 0 2500 1 20 0 30 1 60 0 60 5000 0 60 0 15 0 80 0 30 8 4021 0 5 12 5 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 8 4022 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 8 4023 10 0 0 5 30 4 00 1 20 26 67 10 00 300 0 150 2 00 0 60 5 33 2 00 300 1 60 0 35 2 67 1 00 8 4024 50 0 1 125 6 00 1 10 25 60 9 60 1250 0 625 2 40 0 50 5 12 1 92 1250 1 20 0 30 2 56 0 96 The multichannel pipettes specifications represent the performance of all channels of the pipettes Precision Coefficient of Variation For the extended range different specifications apply 56 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Technical Data VIAFLO II Electronic Pipettes Manufacturer ISO8655 limits Volume Chan Part Bande Incre ed Accuracy Precision Accuracy Precision nel No
9. of with unsorted municipal waste Dispose of the VIAFLO II Electronic Pipettes in accordance with the laws and regulations in your area governing disposal of devices www integra biosciences com 53 VIAFLO II Pipettes Operating instructions VOO Technical Data 7 Technical Data 7 1 Environmental conditions Operation Temperature range 5 40 C Humidity range Max rel humidity 80 for temperatures up to 31 C decreasing linearly to 50 rel humidity at 40 C Altitude range lt 2000 m 7 2 Specification of the device Battery rechargeable Lithium Polymer 3 7 V 1050 mAh Power supply Input 100 240V 50 60Hz Output 6V 0 1 75A DC set at 0 5 A Pipetting channels single 4 6 8 12 or 16 Pipetting speed 10 steps Pipetting technology Air displacement User interface Touch Wheel color display 7 3 Pipetting speed Pipette Size Pipetting speed micro litres per second Speed 12 5 ul 125 ul 300 pl 1250 pl 5000 ul 1 0 44 4 48 11 26 45 16 174 40 2 0 88 8 86 22 29 89 36 345 07 3 2 12 21 38 53 79 215 69 832 93 4 2 56 25 83 65 00 260 63 1006 46 5 3 00 30 24 76 10 305 12 1178 29 6 3 97 40 00 100 65 403 55 1558 39 7 5 35 53 91 135 65 543 91 2100 43 8 6 83 68 89 173 33 695 00 2683 89 9 7 69 77 50 195 00 781 88 3019 38 10 8 79 88 57 222 86 893 57 3450 71 54 INTEGRA Biosc
10. oz for single 100 00135 50 channel 1250 ul and 5000 ul pipettes Grease Grease Nye Fluorocarbon Gel 807 5 g 0 2 oz for single 100 00136 50 channel 12 5 ul 125 ul and 300 ul pipettes Reagent Reservoirs 10 ml Part No Disposable reagent reservoirs individually sealed 4331 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4332 per case sterile Reservoir Base 10 pack 4306 59 www integra biosciences com VIAFLO II Pipettes Operating instructions VOO Reagent Reservoirs 25 ml 100 ml 12 5 ul LONG 12 5 ul 125 ul Accessories Part No Disposable reagent reservoirs individually sealed 4311 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4312 per case sterile Reservoir Base 10 pack 4304 Disposable reagent reservoirs individually sealed 4321 30 reservoirs per case sterile Disposable reagent reservoirs four sleeves of 50 reservoirs 4322 per case sterile Reservoir Base 10 pack 4305 GripTips for all VIAFLO Electronic Pipettes Part No Bulk pack 1 bag of 1000 tips non sterile LONG 4401 5 inserts of 384 tips non sterile LONG GREEN CHOICE 4402 5 boxes of 384 tips non sterile LONG 4403 5 boxes of 384 tips sterile LONG 4404 5 boxes of 384 tips sterile filter LONG 4405 5 inserts of 384 tips pre sterilized LONG GREEN CHOICE 4406 Bulk pack 1 bag o
11. the written consent of INTEGRA Biosciences AG This operating instruction manual has part number 161950 the version is VOO It applies as of see Toolbox Device information Serial number 600000 or higher SW version 3 00 or higher of VIAFLO II Electronic Pipettes until a newer revision is released VIAFLO VIALINK and GripTip are trademarks of INTEGRA Holding Switzerland Manufacturer INTEGRA Biosciences AG INTEGRA Biosciences Corp CH 7205 Zizers Switzerland Hudson NH 03051 USA T 41 81 286 95 30 T 1 603 578 5800 F 41 81 286 95 33 F 1 603 577 5529 info integra biosciences com www integra biosciences com Customer service Please contact your local INTEGRA Biosciences representative To find out name and address of your local representative go to www integra biosciences com Further information and operating instructions in other languages are available on www integra biosciences com or on request info integra biosciences com 6 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Introduction 1 Introduction These operating instructions contain all the information required for installation operation and maintenance of the VIAFLO II Electronic Pipettes This chapter informs about the symbols used in these operating instructions the intended use of the VIAFLO II Electronic Pipettes and the general safety instructions 1 4 Symbols used The operating instructions spe
12. 029 1 0030 1 0031 1 0031 1 0031 21 5 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 1 0032 22 0 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0034 23 0 1 0033 1 0033 1 0034 1 0034 1 0035 1 0035 1 0036 23 5 1 0034 1 0035 1 0035 1 0036 1 0036 1 0036 1 0037 24 0 1 0035 1 0036 1 0036 1 0037 1 0037 1 0038 1 0038 24 5 1 0037 1 0037 1 0038 1 0038 1 0039 1 0039 1 0039 Z values in microliters per milligram 58 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO 8 Accessories Accessories 8 1 Accessories Charging options and Bluetooth Part No Universal voltage power supply 4200 Lithium ion battery 3 7 V 4205 Single pipette charging stand 4210 VIALINK programming stand also for charging 4211 4 pipette charging stand 4215 VIAFLO II Pipette Bluetooth module 4221 Bluetooth PC module with PC software 4225 Universal voltage power supply for 4 pipette charging stand General VIAFLO ASSIST to perform pipetting operations automatically 436 00002 00 Part No 4500 O ring removal tool 8 2 Consumables O rings for tip fittings 130 00731 00 Part No 300 ul Replacement kit 24 pack 100 00027 50 1250 ul Replacement kit 24 pack 100 00028 50 5000 ul Replacement kit 24 pack 100 00029 50 Lubricant for VIAFLO pipettes and O rings Part No Lube Parker Super O Lube Silicone based 50 g 2
13. 10 fast Speed Sets speed of the current pipetting step Operation Press and release the Run Key to initiate the aspiration The total volume aspirated is the sum of desired dispense volume and last dispense volume Depending on whether you press and release or hold the Run Key at the start of a dispense step you can repeat the aspirate and dispense process Press and hold the Run Key Dispense the programmed volume without a blowout keeping the Last Dispense volume in the tip Upon release of the Run Key you can aspirate the programmed volume again and repeat the dispense process as many times as needed Press and release the Run Key Finish the aspirate and dispense process The programmed volume will be dispensed Last Dispense is the next active step To purge the Last Dispense volume with a two step blowout press and hold the Run Key Alternatively if reuse of last dispense is selected you can start the next reverse pipet run with aspirating liquid to the last dispense in the tip www integra biosciences com 33 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 7 Variable dispense mode Application Use this mode when differing dispense volumes are required This mode could be used to quickly set up a dilution series in plates or for feeding similar samples to different assay plates where different sample volumes are needed Options Steps Description Edi
14. Description of the device 2 Description of the device 2 1 Scope of delivery VIAFLO II pipette Rechargeable battery located inside the pipette Li ion 3 7 V 1050 mAh Bag of spare O rings 300 and 1250 ul volume ranges only Accuracy and precision certificate CAUTION Verify the scope of delivery when unpacking the device and check for potential transportation damage Do not operate a device that is damaged instead contact your local dealer 2 2 Pipette configurations VIAFLO Il pipettes couple an easy to use touch wheel controller with intuitive programming They are microprocessor controlled and stepper motor driven www integra biosciences com VIAFLO II Pipettes Operating instructions VOO Description of the device 2 3 Overview of the VIAFLO II Electronic Pipettes 2 3 1 VIAFLO II pipette parts 10 ON DOO fF WD Display Back Button to navigate backward Touch Wheel spin to scroll and move the cursor OK Buiton to make a selection Left and Right Arrow Buttons for selections Purge Button to empty tips Run Key to start operations Tip Ejector Finger Hook facilitates easy operation Volume Indicator Label color matches GripTip box insert Ejector Sleeve Tip Interface INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO 2 3 2 Back view 2 3 3 Display 13 Reset B utton 14 Power Connector 15 Charging Stand Interface 16 Battery The Display show
15. I Pipettes Operating instructions VOO Favorites na Favorites ms Volume 1 300 0 Volume 1 300 0pl Volume 2 250 0pl alan Enn Volume 3 200 0pl Volume 3 Volume 4 1750pl o Volume 5 150 0 Volume 6 1250 Volume 10004 200 0pI HEdit Select 4 4 3 Speed selection The speed option controls the speed at which liquid is aspirated dispensed or mixed in each mode Speed can be set as a value from 1 slowest to 10 fastest see also 7 3 Pipetting speed on page 54 Pipet us Pipet en Press RUN to go Press RUN to go bAspirate 2000pl gt A Operation Use the Touch Wheel to highlight the desired volume and press gt Select Alternatively modify a volume by pressing lt Edit Save your setting D When in any pipetting mode use the Touch Wheel to highlight the Speed option and press OK Select the speed and press OK to save your setting Speed 8 40 0pl Saver Sample Dilute na Sample Dilute my Sample Dilute uaa Press RUN to go Select Step n Select Step PAspirate 1 2000ul 1000 Speed may be changed in most Edit menus Scroll to the Speed and press OK Choose the speed press OK and press to save your selection The speeds selected in each mode i e Pipet Repeat Dispense etc are stored for that mode only Speeds can be set independently for each operation Aspirate Dispense Mix
16. IAFLO II single pipettes can be autoclaved www integra biosciences com 43 VIAFLO II Pipettes Operating instructions VOO Maintenance WARNING A service is required after autoclaving the VIAFLO II pipettes Do not autoclave the entire unit The extreme heat can damage the display and other electrical components Multichannel pipettes can NOT be autoclaved they might be damaged As found calibration measurement report indicating before data is not possible after autoclaving 6 2 1 Autoclaving the disassembled components Place the disassembled components see 6 1 1 to steam autoclave in an autoclave pouch Single channel 3 You may autoclave the components at 121 C 1 bar overpressure for 20 minutes After autoclaving send the pipette for service 6 3 Servicing 6 3 1 Shipping to INTEGRA Biosciences For any service or repairs please contact your local service technician WARNING If working with infectious materials e g human pathogens VIAFLO II pipettes need to be decontaminated before sending them to service and the declaration on the absence of health hazards must be signed This is necessary to protect service personnel 6 3 2 Changing O rings of tip fittings 300 ul 1250 ul and 5000 ul VIAFLO II pipettes feature tip fittings with red O rings This O ring is used to seal against the inside wall of GripTips and serve to reduce the required tip loading and ejecting forces 44 INTEGRA Bioscience
17. INTEGRA VIAFLO II Electronic Pipettes Operating instructions 161950_V00 INTEGRA Declaration of conformity Konformitatserklarung C D claration de conformit Declaraci n de conformidad Dichiarazione di conformit INTEGRA Biosciences 7205 Zizers Switzerland declares on its own responsibility that the product erkl rt in alleiniger Verantwortung dass das Produkt d clare sous sa responsabilit exclusive que le produit declara bajo su propia responsabilidad que el producto dichiara sotto la propria responsabilit che il prodotto VIAFLO Il Electronic Models 4011 4012 4013 4014 4015 4621 4622 4623 Pipettes 4624 4631 4632 4633 4634 4641 4642 in accordance with EC directives gem ss der EU Richtlinien est conforme au terme directives CE de acuerdo con las directivas CE in conformit alle direttive CE 2006 95 EC Low voltage equipment 2004 108 EC Electromagnetic compatibility 2011 65 EC Restriction of Hazardous Substances 2002 96 EC Waste Electrical and Electronic Equipment is in compliance with the following normative documents mit den folgenden normativen Dokumenten bereinstimmt aux documents normatifs ci apr s cumple las documentos normativos soddisfa le normative seguenti EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61326 1 Electrical equipment
18. LINK will detect the pipette automatically 18 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Installation 3 4 4 Device Information Device ee information Description Range Owner Press to enter the user name for your pipette Use the Touch Wheel to highlight a character and press OK You can press lt to Delete the last char acter entered After entering the desired text press gt to Save In addition information about your VIAFLO II Electronic Pipettes such as pipette size indication of single or multi channel serial number software SW and hardware HW version are displayed 3 4 5 Language Language Description Range Language You can choose the language in which all screens are displayed English French German Scroll to the desired language press OK and gt to Save 3 4 6 Write protect Select this option to protect programs and menu options from inadvertent modification The pipetting programs can still be used Write Protect Description Range Select an option and press OK to switch protection On or Off Standard Programs Custom Programs Calibration Toolbox Password Protection Protect the access to the write protect menu by selecting v Edit Password if password protect is switched on To enter a password use the Touch Wheel to highlight a character and press OK Press gt to save t
19. Tips are still in distilled water 4 Remove the GripTips from the liquid and hold the pipette at a 30 angle Wait for 20 seconds a Observe whether liquid droplets are forming at the end of the GripTips b On a multichannel pipette verify liquid level is even across all channels 5 Immerse GripTips approx 2 mm into distilled water and press Run Key to start mix cycle a Observe whether air bubbles are forming when dispensing b On a multichannel pipette check whether the levels stay approximately on the same level across all channels c At the end of a last dispense it is normal to have air bubbles because a blow out is performed Signs indicating a leak 1 During the mix cycle in the leak test the liquid level of 1 channel is decreasing NOTE A decreasing liquid level at aspiration could be an indication of a slow leak Performing a retest at 10 mixes may help identify a slow leak 2 Liquid is left in a tip after the last dispense during the leak test 3 One or more channels show air bubbles during the mix cycle in the leak test 4 Droplets are forming when holding the pipette in the air for 20 seconds even if a pre wet was performed 5 The liquid levels are not equal on all channels after aspiration If the pipette is leaking change O rings see 6 3 2 and lubricate pistons see 6 3 3 or contact your service technician 48 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Maintenance
20. bricatioht iie CS 45 6 4 Calibration seiten arc e tie eed 46 0 4 1 Materials iiie T 46 6 42 Definitions itte eet gest 46 6 4 3 Test Conditions and Environment 47 6 4 4 Pre wetting Of tips maman 47 6 4 5 Leak test iau eee eed 48 6 4 6 Obtaining the Actual Volume eeeeeene 49 6 4 7 Calculation of accuracy and precision 50 6 4 8 Adjusting VIAFLO II Electronic Pipettes 51 6 5 Equipment disposal eene 53 Technical Data 7 1 Environmental conditions cc cccccceeeeeceeceeeeeessseceeeeeeeeeteeeeaes 54 7 2 Specification of the device 54 1 9 PIPCUING Speed iie niet ce belit eee 54 7 4 Intellectual Property 55 7 5 Pipette Specifications sessssseeeeeeeneeennes 56 7 6 Z Cottection Factots mec tee na tanins 58 Accessories BAL 4 ACCOeSSOLIGS s soie etus ec p never eei eeepc pl ce de d dv buses 59 8 2 Consumables ntes Eun IDE 59 www integra biosciences com 5 VIAFLO II Pipettes Operating instructions VOO Imprint 2013 INTEGRA Biosciences AG All rights to this documentation are reserved In particular the rights of reproduction processing translation and the form of presentation lie with INTEGRA Biosciences AG Neither the complete documentation nor parts thereof may be reproduced in any way or stored and processed using electronic media or distributed in any other way without
21. calibration procedure 6 4 2 Definitions Blow out Discharges any residual liquid from the tip Blow in After the blow out the piston moves back into home position This causes a slight intake of air or liquid if tips remain in the liquid Two step blow out To avoid an early blow in keep the run button pressed while dispensing remove the tips from liquid and then release the run button Pre wet The action of pre coating the inside of the liquid contacting parts with a thin film of the same liquid Additionally it equilibrates humidity of the air space inside tip and the pipette Touch off Touching the pipette tip against the surface liquid or side of well plate to release any sample liquids that might be on the end of the pipette tip 400 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Maintenance 6 4 3 Test Conditions and Environment Tests and calibrations should be performed in conditions and environment according to ISO 8655 6 standard Temperature needs to be between 18 25 C and remain constant 0 5 C through out the calibration Optimal relative humidity of the environment is gt 50 and around the dispensing posi tion 8096 GripTips pipettes and distilled water need to be in the calibration laboratory for at least 2 hours prior to calibration to reach temperature equilibrium with the environ ment The balance must be validated using reference weights before and after a measu
22. can potentially damage internal electronics If liquid ever enters the internals of VIAFLO II Electronic Pipettes please contact your service technician Follow the instructions below for disassembly necessary for trouble shooting and autoclaving 6 1 1 Assembly of single channel pipettes Disassembly Disassemble the lower portion of the single channel pipette as follows 1 Ensure the pipette is in the home or down position at the end of a pipetting cycle 2 Turn off power and unplug the power supply 3 Unscrew clockwise to remove the Cylinder Assembly a from the pipette Slide the Cylinder Assembly down from the pipette body This will R expose the Piston Assembly b www integra biosciences com 41 VIAFLO II Pipettes Operating instructions VOO Maintenance 4 Slide the following components from the Piston O Ring black and Seal white Assembly e Seal Retainer Spring d Seal Retainer c Set these components aside or place them in an autoclave pouch 5 Separate the Piston Assembly b from the upper portion of the pipette The Piston is held in place by a small magnet 6 With the Cylinder Assembly a in hand turn counterclockwise to remove the black Sealing Ring f that holds the Ejector Sleeve h in place 7 Remove the Ejector Sleeve h from the Cylinder Assembly 8 Remove the Ejector Sleeve Spring inside g 9 Set all components aside or place them in an auto
23. cate the outer ring of the Cup Seals d e For the 5000 ul pipette also lubricate the bottom of the white Cup Seal www integra biosciences com 45 VIAFLO II Pipettes Operating instructions VOO Maintenance 6 4 Calibration The VIAFLO II pipettes are factory tested and calibrated under environmentally controlled conditions using a gravimetric procedure in accordance with ISO 8655 standards For the accuracy and precision specifications see 7 5 Pipette Specifications on page 56 Several conditions that would require recalibration of your pipette include a desire to Optimize your pipette s accuracy at a specific volume using aqueous liquid Enable accurate displacement of non aqueous based liquids with different specific gravities than water Enhance accuracy when pipetting heated or cooled liquids For information regarding calibration service please contact your local dealer 6 4 1 Materials Precision balance with 0 01 mg readability Ohaus AP250D ASTM Class 2 or OIML E2 test weights Evaporation trap for balance Weighing vessel optimally the height to diameter ratio is at least 3 1 Measurement equipment for temperature humidity and atmospheric pressure Testo 435 2 Professional Distilled water Grade 3 according to ISO 3696 New non filtered GripTips matching the pipette volume see section 8 2 Single pipette charging stand 2 beakers with distilled water 1 for pre wetting 1 for
24. cifically advise of residual risks with the following symbols WARNING This safety symbol warns against hazards that could result in injury It also indicates hazards for machinery materials and the environment It is essential that you follow the corresponding precautions CAUTION This symbol cautions against potential material damage or the loss of data in a microprocessor controller Follow the instructions NOTE This symbol identifies important notes regarding the correct operation of the device and labour saving features 1 2 Intended use VIAFLO Il Electronic Pipettes are microprocessor controlled and stepper motor driven pipettes They are used for aspirating and dispensing liquids in the volume range of 0 5 5000 ul using GripTip pipette tips www integra biosciences com 7 VIAFLO II Pipettes Operating instructions VOO Introduction 1 3 Safety notes VIAFLO II Electronic Pipettes comply to the recognized safety regulations and is safe to operate VIAFLO II Electronic Pipettes can only be operated when in perfect condition and while observing these operating instructions The device may be associated with residual risks if it is used or operated improperly by untrained personnel Any person operating the VIAFLO II Electronic Pipettes must have read and understood these operating instructions and particularly the safety notes or must have been instructed by supervisors so that safe operation of the device is guarante
25. cing pipetting results These techniques are consistent with ISO standard 8655 2 e It is best to immerse the GripTips just enough in liquid 2 3 mm to allow the desired volume to be aspirated Always prewet GripTip pipette tips After loading tips onto your pipette aspirate and dispense the full volume 2 3 times to coat the inside of pipet tips Pre wetting ensures that the liquid and air inside the tips are at equal temperature and the dead air space is humidified VIAFLO II Electronic Pipettes is an air displacement pipette To properly dispense liquids ensure that the pipette tip is at a 0 30 angle against the wall of the container or well After dispense you must touch GripTip pipette tips against wall or dip them into the liquid after a dispense This process is referred to as touching off or tip touch and prevents liquid from clinging to the pipette tips n programs such as Repeat Dispense a first and last dispense can be programmed These two dispenses are not used and are dispensed into the waste as they contain the accumulated pipetting errors Using a first and last dispense is recommended if accuracy and precision are of high importance Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting In addition the pipetting mode Reverse pipet can be used to optimize pipetting results with viscous samples For pipetting liquids with high vapor pressures such as methan
26. clave pouch Reassembly Before reassembling the pipette check each component for lint or dust particles It is recommended to replace the O Ring black and Seal white Assembly e before reassembly The O Ring and Seal slide on off the piston and can be replaced easily 1 Lightly lubricate the piston and seals see 6 3 3 Lubrication on page 45 Position the Piston b at the small magnet to connect the Piston to the upper portion of the pipette 2 Slide the Seal Retainer c onto the Piston and up into the pipette The end of the Seal Retainer that has an extended edge lip should be closest to the upper portion of the pipette over the magnet 3 Slide the Seal Retainer Spring d onto the Piston It will rest against the Seal Retainer 4 Slide the lightly lubricated Seal white and O Ring black Assembly c onto the Piston The white Seal will be closest to the Seal Retainer Spring Be sure the black O Ring is securely in place on the white Seal 5 With the Ejector Sleeve h in hand slide the Ejector Sleeve Spring g onto the top of the Sleeve 6 Slide the Ejector Sleeve into the Cylinder Assembly a 7 Position the black Sealing Ring f over the Ejector Sleeve h at the top of the Cylinder Assembly Turn clockwise until the Sealing Ring snaps into place and secures the Ejector Sleeve in the Cylinder Assembly 8 Slide the Cylinder Assembly over the Piston 82 INTEGRA Biosciences AG VIAFLO II Pipettes
27. com 15 VIAFLO II Pipettes Operating instructions VOO 3 4 1 Preferences Installation Preferences customizes your system parameters Select a preference and press OK to access Preference Description Range Sound Simple tones indicate completion of operations and errors Select an option and press OK to change the status of the beep tone between On and Off Step Complete At the end of a program step Program Complete At program completion Purge Key When Purge is pressed Messages When a message appears Error Message When an error message appears or when illegal data entry is attempted Touch Wheel When spinning the Touch Wheel Last Dispense Before the last dispense in Repeat Dispense and Variable Dispense vx On Off Display Customizes your display Press OK to select an option use the Touch Wheel to display the desired value Start up Screen Select the startup display at one of the following None or Custom up to two uploaded with VIALINK Press gt to save your selection Brightness Use the Touch Wheel to change the brightness 1 dim to 10 bright Press OK Dim Time The display will dim after a set number of minutes A shorter dim time helps preserve battery life Press OK to save your selection Standby Time The display will turn off after a set num ber of minutes and allows you to continue working where you have left A shorter standby ti
28. cted independently to Dispense improve accuracy and precision The dispense is discarded Last Dispense A last dispense volume can be selected independently to improve accuracy and precision The dispense is discarded Reuse Last By default the mode ends with dispensing of the last Disp dispense red X If you want to reuse the last dispense press OK green tick the last dispense will remain in the tip Count The maximum number of dispenses possible count is calculated automatically This count may be reduced to the desired number Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Disp Speed Sets speed uniquely for dispensing 1 slow 10 fast Speed Sets speed of the current pipetting step Pace Sets the time duration between dispenses if keeping Run Key pressed Operation With the tip s in liquid press and release the Run Key to initiate the aspirate step Press and release the Run Key for every dispense Alternatively press and hold Run to execute paced dispenses The dispense number is shown on the display The pipette will stop paced dispenses when it reaches the Last Dispense This aliquot contains the accumulated error from all prior dispenses You can choose to use this Last Dispense or discard it During the Last dispense press and hold the Run Key to perform a two step blowout Alternatively if reuse of last dispense is selected you can start th
29. ction between temperature and air pressure in Table 7 6 Round up temperature and air pressure values Multiply each weight m obtained in 6 4 6 with the corresponding Z factor V m xZ l Add together the volumes V delivered divide the sum by n e g n 5 to calculate the mean volume V in milliliters or micro liters delivered at the test temperature which is the Actual Volume V 3 Iie n x Y V i 1 50 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Maintenance Calculation of systematic error Accuracy The systematic error e can be calculated using the following equation with V being the selected test volume or in percent 100 x Vx V Ss e Calculation of random error Precision To calculate the random error as the repeatability standard deviation s use the following equation The random error can also be expressed as a percentage by the coefficient of variation CV using equation M CV 100 x V If the pipette is properly calibrated the Target volume V should equal the Actual volume V within the accuracy specifications of the pipette 6 4 8 Adjusting VIAFLO Il Electronic Pipettes Compare the calculated accuracy and precision values with the corresponding pipette specifications given in section 7 5 In case a VIAFLO II Electronic pipette has not met the calibration specifications it needs to be adjusted This can easily be corrected by the pipette s
30. d Unit does not Make sure the charger is turn on when placed plugged in on the charging stand Error message Indicates too much friction Reset the pipette Homing error is was encountered during f problem persists please con displayed operation tact service for technical assis Indicates possible motor fail tance ure www integra biosciences com 27 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 Pipetting modes This chapter describes how to program the VIAFLO II Electronic Pipettes in two ways Function based pipetting modes You can select from ten predefined pipetting modes that you can quickly and easily edit and execute They are described in the fol lowing sections Custom step based programming mode You can create and store up to twenty multi stepped pipetting protocols on the pipette using the five basic functions of Aspi rate Mix Dispense Purge and Prompt presented in 5 2 Detailed description of pipetting modes on page 29 The custom programming mode is described in 5 3 Custom step based programming mode on page 38 5 1 Overview pipetting modes The table below provides an overview of the selectable pipetting modes All modes are accessed from the Main Menu Use the Touch Wheel to scroll to your desired pipetting mode Pipetting mode Description Pipet Allows liquid transfers when aspirate and dispense volumes are equal
31. e next repeat dis pense run with aspirating liquid to the last dispense in the tip 30 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 3 Sample dilute mode Application Accomplish accurate sample dilutions by using diluent to chase small sample volumes from the pipet tips An air gap keeps liquid separated in the tips and helps to minimize carryover of diluent when aspirating the sample Options Steps Description Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip Air Gap Sets the volume of the air gap to keep both liquids separated Aspirate 2 Sets the volume of the sample in the tip Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Disp Speed Sets speed uniquely for dispensing 1 slow 10 fast Speed Sets speed of the current pipetting step Operation Press and release the Run Key to initiate each aspiration remove tips from liquid for air gap aspiration Press and hold the Run Key to perform a two step blowout The entire tip contents will be dispensed together 5 2 4 Pipet mix mode Application Use this mode when mixing is required immediately after transfer of liquid This mode saves a programming step by incorporating the mix option after dispensing Options Steps Description Edit Aspirate Sets the aspiration volume that is equal to the dispense volume Mix Sets the mix
32. e set up as followed Program step Action 1 Aspirate liquid 1 160 ul e g diluent With tips in liquid 1 press Run Key 2 Aspirate air 20 ul Move tips out of liquid and press Run Key 3 Aspirate liquid 2 50 ul e g reagent With the tips in liquid 2 press Run Key 4 Dispense 230 ul Press and hold Run Key until liquid is dispensed and tips are removed from the liquid two step blowout 5 Mix 3x 200 ul Press Run Key Purge does not need to be programmed The residual liquid is dispensed into the waste container Press and hold Run Key until liquid is purged and tips are removed from the liquid two step blowout For a detailed description see 4 3 2 Blowout modes on page 21 40 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Maintenance 6 Maintenance WARNING Always turn off power and disconnect the VIAFLO II Electronic Pipettes from the mains when carrying out maintenance work 6 1 Cleaning The materials used on the exterior of the VIAFLO II Electronic Pipettes support regular cleaning intervals Clean the external components with a lint free cloth lightly soaked with mild soap solution in distilled water or with a 70 dilution of Isopropyl or Ethanol Never use acetone or other solvents A WARNING A Do not immerse the entire pipette into a cleaning solution or spray cleaning d solution directly onto the exterior body of the pipette as this
33. ed Use only an INTEGRA battery 4205 Regardless of the listed safety notes additional applicable regulations and guidelines of trade associations health authorities trade supervisory offices etc must be observed Do not open or modify the VIAFLO II Electronic Pipettes in any way Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after sales service member Parts may be replaced with original INTEGRA Biosciences parts only WARNING Do not use the VIAFLO II Electronic Pipettes near flammable material or in explosive areas Also do not pipette highly flammable liquids such as acetone or ether When handling dangerous substances comply with the material safety data sheet MSDS and with all safety guidelines such as the use of protective clothing and safety goggles CAUTION Do not immerse the VIAFLO II Electronic Pipettes in liquid The fluid can damage internal parts Avoid pipetting of liquids whose vapors could attack the materials PA polyamide POM polyoxymethylene FPM fluor rubber NBR nitrile rubber CR chloroprene silicone Corrosive vapors could also damage metallic parts inside the device NOTE Prolonged exposure of the VIAFLO Il Electronic Pipettes to UV light can cause discolouration and or yellowing of the control unit However this will not affect the performance of the device in any way 8 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO
34. egrated at the end of a program if the last programming step leaves liquid in the tips Prompt A prompt is any message that is displayed during the program Use the Touch Wheel to select one of 3 lines and press OK Highlight a character that you want to use and press OK After you enter the desired text press gt to Save To save and store a Custom program press Save gt To run the program press OK 5 3 2 Modify existing programs Custom Co View Edit Delete Newb At the Custom program display use the Touch Wheel to highlight an existing program and press lt Options Select an option View Edit Delete Copy Rename to modify the program With the View Edit option you can always add a new step edit a step or delete a step CUSTOMT eus 1 Aspirate 300pl 8 100pl 8 2 Dispense 3 10 01 8 Delete Step To insert a new step press lt Edit select New Step and press OK Use the Touch Wheel to select the position where a new step should be inserted and press OK Select an operation and press OK Press Save gt to return to the list of Custom programs www integra biosciences com 39 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 3 3 Example of custom mode Application The task is to combine 2 different liquids in a 96 well plate for a kinetic assay and then mixing it to achieve a homogenous solution The custom program would b
35. essel to perform a 2 step blowout Record the weight from the balance After completing the 5 high volume measurements continue with the mid and low test measurements by repeating steps 2 6 www integra biosciences com 49 VIAFLO II Pipettes Operating instructions VOO Maintenance 6 4 7 Calculation of accuracy and precision Accuracy The ability of a pipette to aspirate or dispense the exact volume desired It indicates the proximity of measurement results to the true value Accuracy is also know as systematic error and as the name indicates can be corrected calibrated Precision Coefficient of variation CV Precision indicates the repeatability or reproducibility of the measurement It is also called random error and is therefore an unpredictable error which can not be corrected calibrated EN The following symbols are used throughout this text Target V Actual V qu Selected test or target Volume Measured Liquid Weight g Accuracy Z factor see 7 6 Converted Volume ml or ul Actual mean Volume ml or ul S lt lt NE Il Number of measurements Precision Conversion of the mass to volume The values obtained by balance readings are in grams These values need to be converted to micro liters using the Z correction factor It takes into account the water density and air buoyancy during weighing at the corresponding test temperature To determine the correct Z factor find the interse
36. essories on page 59 3 2 1 Charging the battery on a stand Use one of the different charging stands single position with two 4210 or four prong connector 4211 or four position with two prong connector 4215 to charge the battery i r Place the pipette on the charging stand by fitting the 4 f power receptacle 15 on the top back of the pipette over the prong connector on the top of the stand Plug the cable of the adequate power supply to the J I socket at the base of the stand see 8 1 Accessories i t on page 59 ew CAUTION STOP Always use the correct power supply for the charging stand 14 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Installation The pipette will turn fully on when placed on the stand When the dimming time is achieved the startup screen is displayed on the pipette When the turnoff time is reached the pipette turns off For disconnecting simply lift the pipette up from the stand 3 2 2 Charging the battery with Universal Power Supply With the optional Universal Voltage Power Supply UPS 4200 you can use the pipette while charging through the line cord Insert the Universal Power Supply connector into the receptacle on f the top back of the pipette 14 Plug the Universal Power Supply into a wall outlet The pipette will turn fully on when the line cord is connected The j current screen remains displayed If the pipette is idle wh
37. f 1000 tips non sterile 4411 5 inserts of 384 tips non sterile GREEN CHOICE 4412 5 boxes of 384 tips non sterile 4413 5 boxes of 384 tips sterile 4414 5 boxes of 384 tips sterile filter 4415 5 inserts of 384 tips pre sterilized GREEN CHOICE 4416 Bulk pack 1 bag of 1000 tips non sterile 4421 5 inserts of 384 tips non sterile GREEN CHOICE 4422 5 boxes of 384 tips non sterile 4423 5 boxes of 384 tips sterile 4424 5 boxes of 384 tips sterile filter 4425 5 inserts of 384 tips pre sterilized GREEN CHOICE 4426 60 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Accessories 300 pl Bulk pack 1 bag of 1000 tips non sterile 4431 5 inserts of 96 tips non sterile GREEN CHOICE 4432 5 boxes of 96 tips non sterile 4433 5 boxes of 96 tips sterile 4434 5 boxes of 96 tips sterile filter 4435 5 inserts of 96 tips pre sterilized GREEN CHOICE 4436 1250 ul Bulk pack 1 bag of 500 tips non sterile 4441 5 inserts of 96 tips non sterile GREEN CHOICE 4442 5 boxes of 96 Tips non sterile 4443 5 boxes of 96 Tips sterile 4444 5 boxes of 96 Tips sterile filter 4445 5 inserts of 96 tips pre sterilized GREEN CHOICE 4446 5 ml Bulk pack 1 bag of 250 tips non sterile 4451 Individually wrapped 100 tips per case sterile 4456 61 www integra biosciences com
38. fast Disp Speed Sets speed uniquely for dispensing 1 slow 10 fast Speed Sets speed of the current pipetting step Operation With the tip s in liquid press and release the Run Key to initiate the aspiration of the reagent Submerge the GripTip pipette tips in the liquid located in the first row of the plate Press and release the Run Key to start the dispense and mix sequence Proceed with the rest of the rows Rows first number and Mix Cycles second number are tracked on the display Mix Cycles are shown in red when mixing A green dot on the row number indicates the active program step www integra biosciences com 37 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 3 Custom step based programming mode Application Use the Custom program mode to create personalized pipetting tasks Up to twenty programs can be stored 5 3 1 Create a custom program From the Main Menu select Custom to create a personalized protocol Programs can contain up to 98 individual steps based upon the following basic operations Aspirate Dispense Mix Purge and Prompt NOTE We recommend creating custom programs on a PC with the VIALINK software see also 3 4 3 Communications on page 18 Custom Input Name i CUSTOM Press New P to create a new program Your are lo Programs ABCDEFGHI J prompted to enter a name KLMNOP QRST UVWXYZ u Use the Touch Wheel to select charac
39. he pass word The password must be entered before you can access the write protect menu v x On Off Keep the password in a safe place Should you lose your password contact INTEGRA Biosciences to retrieve your password www integra biosciences com 19 VIAFLO II Pipettes Operating instructions VOO Operation 4 Operation 4 1 Turn on off the device Turn on Press and release the Run Key 7 to turn on the pipette CAUTION Remove hands from the tip interface 12 at switch on and during homing Also do not touch the touch wheel because it is calibrated during the start up process The pipette flashes the startup screen and performs a full motor homing routine ensuring the motor is in the run position Home is the base point for the pipette Homing is the process whereby the pipette motor moves the piston s to a sensor position This position ensures that no liquid remains in the tips After homing the Main menu is displayed Turn off To turn off the pipette press and hold the Back Button 2 for 3 seconds NOTE The VIAFLO II Electronic Pipettes will dim and turn off automatically after a preset duration of inactivity This duration is 5 minutes by default and configurable with the Toolbox see 3 4 1 Preferences on page 16 4 2 Attaching and removing GripTip pipette tips CAUTION To ensure optimal performance of your VIAFLO II Electronic Pipettes always use GripTip pipette ti
40. ibration amp Services These options enable you to set calibration features and review service history Pipettes to restore accuracy The calibration factors for Pipette and Repeat type are displayed To edit the calibration volumes press 1 Target Volume This is the volume you are interested in using for the calibration Actual Volume This is the measured volume obtained when dispensing the target volume Current Factor Displays the factor currently in use Factory Reset Resets the correction factor back to the original factory setting Press 1 to apply the factory setting Calibration NC g Services Description Range Calibration Allows for re calibration of the VIAFLO II Electronic www integra biosciences com 17 VIAFLO II Pipettes Operating instructions VOO Installation Calibration Sets a calibration reminder based on a specified time Reminder frame or number of pipetting cycles When the calibration reminder is displayed press any key to confirm Time or However the reminder will reappear every time the Cycles pipette is turned on until you change the reminder time or use the reset option Reminder Press OK to turn the reminder timer On or v x Off On Off Days Cycles Use the Touch Wheel to set a reminder 1 365 days interval for calibration time in days or in thousands of jor 1k 240k cycles Press lt to set the timer to the defined calibra cycles t
41. id High viscosity liquids may require calibration High vapor pressure liquids may require pre wetting No dispensing aspirating Piston stuck or not con nected Motor not running O ring not installed Contact service Droplets on the tips 26 Temperature of liquid differs from that of air inside the tips Liquid of low viscosity and high vapor pressure Pre wet tips up to 3 times Increase dispensing speed INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Operation 4 5 2 Electronic Problem Probable cause Remedy When pressing Run Low battery Re charge the battery in order to a Low Battery resume pipetting operation message appears Plug the power cord into the on the Run screen pipette Display turns off Dead battery Charge the battery with a power completely cord or charge stand Spinner response is A finger was on the spinner Reset the pipette without touch erratic and uncon when the pipette was turned ing the spinner during reset trollable on Sensitivity setting is not set Adjust the spinner sensitivity with correctly Toolbox Preferences Spinner see 3 4 1 Displayed charac Unknown Reset the pipette ters are scrambled Battery charging Charge stand pins are out of Check that both charge stand indicator is not pul place conductor pins are at the same sing while on the height stan
42. iences AG VIAFLO II Pipettes Operating instructions VOO Technical Data 7 4 Intellectual Property The VIAFLO II Electronic Pipettes are covered under the following patents Patent Number Country Title Apply to 7 662 343 USA Locking Pipette Tip And Mounting All pipettes Shaft 7 662 344 USA Locking Pipette Tip And Mounting GripTip Tip fitting Shaft 5261392 JAPA Locking Pipette Tip And Mounting GripTip Tip fitting Shaft 8 033 188 USA Pipettor Software Interface All pipettes 2192985 EPC Pipettor Software Interface All pipettes FRAN GBRI SWIT 602008010945 GERM Pipettor Software Interface All pipettes D596 754 USA Pipette All pipettes 7 540 205 USA Electronic Pipette Assembly All pipettes 8 122 779 USA Electronic Pipettor With Improved All pipettes Accuracy D599 030 USA Multi Channel Pipette Multichannel pipettes 7 811 522 USA Sample Reservoir Kits With Dispos Reservoirs able Liners D599 031 USA A Liquid Sample Or Liquid Reagent Reservoirs Reservoir Kit 8 277 757 USA Pipette Tip Mounting Shaft GripTips 8 501 118 USA Disposable Pipette Tip GripTips www integra biosciences com 55 VIAFLO II Pipettes Operating instructions VOO Technical Data 7 5 Pipette Specifications VIAFLO II Electronic Pipettes Manufacturer ISO8655 limits Volume Chan Part Rade Incre ved Accuracy Precision Accuracy Precision nel No
43. ile charging the display may dim see 4 1 Turn on off the device on page 20 but it will continue to show the battery life indicator When the display shut off time is reached the instrument will shut off 3 3 Exchanging the battery To exchange the battery for any reason loosen the screw to detach pipette backing Disconnect the power plug of battery 16 Connect the power plug with the new battery and reassembly the pipette backing After exchanging the battery a protective switch is active The pipette can only be started when connect to the mains power supply 3 4 Toolbox adapt your VIAFLO II Electronic Pipettes The Toolbox provides options to adapt the device to appropriate applications setting personal preferences calibration computer connectivity and storing device information Toolbox mode Description ASSIST Additional pipetting modes available in combination with VIAFLO ASSIST hidden by default see Preferences Main Menu Preferences Customizes the system parameters Calibration amp Service Sets calibration and service history options Communications Enables communication between your VIAFLO II Electronic Pipettes and a PC Device Information View your pipette s serial number and set a personal ID Language Sets language Write Protect Protects programs or menu options from modification Help information is available for each mode www integra biosciences
44. ines up to 10 favorite volumes Operation When aspirating the motor will stop when you release the Run Key or when the pro grammed aspirate volume is reached You can change pipetting direction at any time even if aspiration volume is not reached Change the direction of pipetting by pressing OK on the Direction option The notation on the display changes between A Aspirate and V Dispense Titrations can be performed by dispensing in this mode The volume remaining in the tip s is always actively displayed NOTE Use slower pipetting speeds 1 5 for better control and resolution 32 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 6 Reverse pipet mode Application Use this mode when performing reagent addition where a blowout is not desired when dispensing e g for solutions with high viscosity or tendency to foam The dispense method used in this mode prevents introduction of any air into the sample Options Steps Description Edit Dispense Sets the aspiration and dispense volume Last Dispense Sets the volume to leave in the tip until final blowout Reuse Last By default the mode ends with dispensing of the last Disp dispense red X If you want to reuse the last dispense press OK green tick the last dispense will remain in the tip Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Disp Speed Sets speed uniquely for dispensing 1 slow
45. ing volume after dispensing Mix Cycles Sets the number of mix cycles Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Mix Speed Sets speed uniquely for mixing 1 slow 10 fast Speed Sets speed of the current pipetting step Operation Press and release the Run Key to initiate the aspiration Press and release the Run Key to dispense Mixing occurs automatically after the dis pense step Upon completing the desired number of mixes a blowout is initiated automatically prompting you to remove the tip s from the liquid and press Run to complete the blowout www integra biosciences com 31 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 5 Manual pipet mode Application This mode can be used when the aspiration volume is not defined or unknown You have control over the aspiration and dispense steps and can view the display to confirm how much liquid has been aspirated or dispensed Manual control over the dispense steps is perfect for performing Titrations or for controlling the loading of samples in gel lanes Options Steps Description Edit Aspirate Sets the aspiration or dispensing volume Toggle between Aspirate and Dispense using the Direction menu option Speed Sets speed of the current pipetting step 1 slow 10 fast Direction Changes the direction of pipetting between aspiration and dispensing Favorites Def
46. ion interval Remind in Total Cycles Displays the residual time or amount of cycles respectively before calibration is required Reset Resets the timer to the defined calibration inter val Press lt to enable Press gt to save Service Displays notes of any service that took place on the a History VIAFLO II Electronic Pipettes listed newest entry first After changing desired settings press gt to save 3 4 3 Communications The VIAFLO II Electronic Pipettes can be programmed from a PC via a programming stand 4211 or via wireless Bluetooth connection VIALINK is a pipette management software for the PC and is used to manage VIAFLO II Electronic Pipettes It can be downloaded from the INTEGRA website in the product section and is free of charge for all VIAFLO II Electronic Pipettes customers A detailed description of the software along with the operating instructions can be found on the website as well Communi cations Description Range VIALINK Place the pipette on a VIALINK programming stand USB Stand 4211 and connect it to the USB port of your PC To exit the communications mode follow screen prompt or reset the VIAFLO II pipette 13 VIALINK Each pipette needs its own Bluetooth module 4225 Bluetooth Bluetooth communication has an open field connectivity of about 10 meters Select one connection path and press OK to allow communication with the PC VIA
47. ix Cycles second number are tracked on the display Mix Cycles are shown in red when mixing A black triangle on the column number indicates the active program step Select a desired value Press OK and then press gt to save your setting s 4 4 6 Help The Help information available for each mode describes the mode operation Main Menu a Repeat Dispense While in the Main menu highlight a pipet mode then Sample Diluti Pinet MI press lt to select the Help option Manual Pipet Reverse Pipet Variable Dispense ElHelp Runt www integra biosciences com 25 VIAFLO II Pipettes Operating instructions VOO 4 5 Troubleshooting FAQ 4 5 1 General Problem Pipette leaks or does not fill with Probable cause O ring was not properly installed Operation Remedy Replace the tip fitting O ring see 6 3 2 liquid after During reassembly the O ring reassembly was damaged Leakage Tip incorrectly attached Attach a new tip Foreign particles between tip and tip cone Foreign particles between the piston the O ring and the cylinder Damaged red O ring Clean tip cones Attach new tips Change the red O ring see If leak persists contact service Dispense results are inaccurate Unsuitable calibration Incorrect aspirate and dis pense speed Recalibrate with the liquids in question Adjust aspiration and dispense speed depending on liqu
48. me helps pre serve battery life Turn Off Time By default the pipette will turn off after 5 minutes of idle time You can change this setting Press OK to save your selection None Custom 1 or 2 1 10 Never 1 20 min 5 60 min 1 24 hours Main Menu Select a function to be hidden from the main menu Off and press OK e g ASSIST Pipet Repeat Dispense Sample Dilute Pipet Mix vx On Off Touch Wheel Adjust your Touch Wheel spin sensitivity Press gt to save Low Medium High 16 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Installation Pipetting Select an option and press OK Purge Key Speed Choose the desired purge speed 1 10 and press OK to save your selection Blowin Delay Choose a timed delay between the None blowout and the blowin delay before the piston 0 5 4 1 5 s homes at the end of a dispense if no two step blowout is performed see 4 3 2 Blowout modes on page 21 Extended Volume For pipetting below or above the v x volume range specified On Off 125 ul pipette 2 0 5 125 ul 300 ul pipette 5 0 10 300 310 ul 1250 ul pipette 25 50 1250 ul The volumes in brackets refer to extended volumes e g extend the minimal pipetting volume on a 125 ul pipette from 5 ul to 2 ul Not available for 12 5 ul and 5000 ul pipettes After changing desired settings press gt to save 3 4 2 Cal
49. ments 496 S96 496 S96 ul ul pl 12 4081 0 5 12 5 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 12 4032 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 12 4033 10 0 0 5 30 4 00 1 20 26 67 10 00 300 0 150 2 00 0 60 5 33 2 00 300 1 60 0 35 2 67 1 00 12 4034 50 0 1 125 6 00 1 10 25 60 9 60 1250 0 625 2 40 0 50 5 12 1 92 1250 1 20 0 30 2 56 0 96 16 4041 65 425 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 16 4042 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 The multichannel pipettes specifications represent the performance of all channels of the pipettes Precision Coefficient of Variation For the extended range different specifications apply www integra biosciences com 57 VIAFLO II Pipettes Operating instructions VOO Technical Data 7 6 Z Correction Factors Temp Air Pressure kPa C 80 85 90 95 100 101 3 105 18 0 1 0022 1 0023 1 0023 1 0024 1 0025 1 0025 1 0025 18 5 1 0023 1 0024 1 0024 1 0025 1 0025 1 0026 1 0026 19 0 1 0024 1 0025 1 0025 1 0026 1 0026 1 0027 1 0027 19 5 1 0025 1 0026 1 0026 1 0027 1 0027 1 0028 1 0028 20 0 1 0026 1 0027 1 0027 1 0028 1 0028 1 0029 1 0029 20 5 1 0027 1 0028 1 0028 1 0029 1 0029 1 0030 1 0030 21 0 1 0028 1 0029 1 0
50. ol or ethanol use relatively fast pipetting speeds and avoid prolonged pauses after aspiration Calibrate based on fluid type VIAFLO II Electronic Pipettes is tested and calibrated at the factory for use with distilled water at room temperature It may be necessary to re calibrate your VIAFLO II Electronic Pipettes if the liquid to be used has different physi cal properties specific gravity and vapor pressure than water Calibration mode can be accessed in the Toolbox menu WARNING Avoid pipetting for extended periods To minimize the risk of repetitive strain injury include pauses of several minutes 22 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Operation 4 4 Pipetting options and settings 4 4 1 Edit option The Edit option is available for each mode It enables you to access the variables that you can adjust for a pipetting mode These variables include Speed Volume Pace Count Mix Cycles Rows and Direction Additional steps include First dispense Last dispense Air gap Aspirate speed Dispense speed etc Repeat Dispense Rm RES Select a pipetting mode Then select Edit on the list Mr Eux of options and press OK A list of associated steps is Lesbkpeme tou displayed For example if selecting Edit on the Le n Repeat Dispense screen the modifiable steps mon Disp Speed e associated with Repeat Dispense are displayed ElFavorites Savei 4 4 2 Volume
51. pette The routine ends by displaying the Main Menu www integra biosciences com 13 VIAFLO II Pipettes Operating instructions VOO Installation 3 Installation 3 1 Operating environment The VIAFLO II Electronic Pipettes have been designed for use in a laboratory They shall be operated in a dry and dust free location with a temperature of 5 40 C and a maximal non condensing relative humidity of 80 3 2 Charging the battery All VIAFLO II hand held pipettes share the same rechargeable long life lithium ion battery that has a capacity of 1050 mAh The battery should be charged for at least one hour before pipetting A full charge takes 2 5 hours and will provide approximately 3500 pipetting cycles A battery indicator is provided on the pipette display 2 3 3 indicating different states Blinking red icon Low battery A recharging message will appear Green icon Pipette is connected to the power supply Blinking bars Pipette is recharging Two static and one blinking bar on the right Pipette is recharging and battery is 8096 charged Three static bars Battery is fully charged CAUTION Use only the approved INTEGRA battery power supply or charging stand Use of an incompatible power transformer can damage the pipette Battery charging can take place in two ways using a pipette charging stand or the Universal Voltage Power Supply UPS that allows for pipetting while charging the battery see 8 1 Acc
52. ps which have been designated for the use with the VIAFLO II Electronic Pipettes see 8 2 Consumables on page 59 The unique lobe design of the VIAFLO II Electronic Pipettes reduces attachment and ejection forces ensures a perfect fitting to prevent the tips from falling off and to provide a perfect sealing On a multichannel pipette all tips sit on the same height Lobes minimize contact surface and temporarily deforms the tip shaft Shoulder provides a positive stop to prevent over tightening and all tips seal at the same height O ring provides forgiving and robust seal surface for the pipet tip 20 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Operation Attach the tips When loading tip s press the pipette into the appropriate GripTip s until you hear and feel a click indicating that a seal has been achieved Once you feel the click stop applying pressure When loading GripTip pipette tips using a multichannel pipette slowly rock the pipette from one side to the other side to ensure the proper seal is achieved Discard your used tips If liquid is in the tips empty them by pressing the Purge Button 6 Tips are easily removed by pressing the Tip Ejector 8 43 Start pipetting 4 3 1 Pipetting Use the Touch Wheel 3 to scroll to your desired pipetting mode and press OK 4 Selected parameters for the action you are about to perform will be displayed on the Run Screen
53. ring series A low and high volume weight should be used E g 100 g Mettler Toledo 11119250 10 g Mettler Toledo 11119220 1 g Mettler Toledo 11119190 10 mg Mettler Toledo 11119130 CAUTION STOP Always use new pre wetted GripTips for leak test and calibration 6 4 4 Pre wetting of tips Pre wet new GripTips three times prior to starting tests and calibrations This is required every time a tip is changed 1 Attach a new unused GripTip which correspond to the volume range of the pipette directly from the rack without touching the tip by hand 2 Use a separate waste container for pre wet dispenses 3 Inthe Pipet mode program the pipette to aspirate full volume Set aspirating speed at 6 Aspirate by pressing the Run Key 4 Dispense Keep the Run Key pressed until all liquid is expelled remove the tip from the liquid and then release the Run Key to perform a two step blowout see 4 3 2 Blowout modes on page 21 www integra biosciences com 47 VIAFLO II Pipettes Operating instructions VOO Maintenance 6 4 5 Leak test It is recommended to perform a leak test every 3 months or when errors occur 1 Prewet tips as described above 2 In the Pipet Mix mode program the VIAFLO II Electronic Pipettes to aspirate full volume at speed 6 and mix at full volume Set to mix to 6 cycles and set mixing speed at 6 3 Aspirate full volume and verify liquid level is not decreasing during 2 3 seconds while Grip
54. s AG VIAFLO II Pipettes Operating instructions VOO Maintenance O rings are made of durable silicone If necessary e g in case of a leakage due to damaged O ring you can replace these O rings A set of spare O rings and an O ring removal tool are included with the large volume multi channel pipettes and can be ordered separately see 8 Accessories on page 59 WARNING Avoid mechanical damage of the tip fittings For 300 ul or 1250 ul pipettes choose the side of the O ring removal tool corresponding to the size of the pipette cylinder 300 ul or 1250 yl Slide the O Ring removal tool sidewards onto the tip fitting until the O ring a builds a loop Cut the O ring with a fine scissor and remove it For 5000 ul pipettes use forceps to remove the O ring Slide a new O ring over the tip fitting b 6 3 3 Lubrication The internal seals and O rings are subject of very slow wear out An undamaged thin lubricant film is important to keep the seals tight The lubricant recommended depends on the VIAFLO II pipette size see 8 2 Consumables on page 59 Single channel 12 5 pl 125 pl 300 pl C Use Fluorocarbon Gel 100 00136 50 to lightly lubricate the b piston a without components slided onto Put a drop of grease onto your fingers lubricate the black O ring b at slide it over the white seal c Single channel 1250 pl and 5000 ul Use the Super O Lube 100 00135 50 to lightly lubri
55. s all pipetting options INTEGRA Description of the device Instruction ee __ gt Volumes gt Options Main Menu ma Battery Life Indicator Repeat Dispense Sample Dilute Pipet Mix Manual Pipet Reverse Pipet Variable Dispense OK Hel Runt Current mode Piet a Press RUN to go Active step asnirate 250 01 Speed 8 Favorites www integra biosciences com TT 11 VIAFLO II Pipettes Operating instructions VOO Description of the device 2 3 4 Touch wheel The Touch Wheel is fully operational with only one hand Rotational finger movements translate into up and down cursor movement on the display The Touch Wheel is fully functional with the use of latex gloves Favorites m Favorites Move finger on the Touch Wheel to choose and Volume 1 00 0 Volume2 2500p jaune nou highlight an option on the display Press OK 4 to Mos ME e MES make the selection Volume 5 1500 r r 7 Volume 6 1250 200 0yl When a setting dial is displayed spin the Touch ee ER Wheel to change the value and press OK 2 3 5 Left and right arrow buttons Main Menu a At times you will see lt and gt on a display screen These buttons Sed ig are used to select options ample Dilute ch ot Press lt to select the option indicated with the left arrow HELP in Reverse Pipet the example beside Press gt to select
56. s speed uniquely for aspirating 1 slow 10 fast Mix Speed Sets speed uniquely for mixing 1 slow 10 fast Speed Sets speed of the current pipetting step Operation With the tip s in liquid press and release the Run Key to initiate aspiration 1 With the tip s out of the liquid press and release the Run Key for the Air Gap Again in liq uid press and release the Run Key to initiate aspiration 2 Press and release the Run Key to dispense the entire tip contents and begin the mix ing routine Upon completing the desired number of mixes a blowout occurs automat ically Remove tips from liquid and press and release the Run Key to complete the blowout 36 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 10 Serial dilution mode Application Use this mode to perform serial dilutions The Serial Dilution mode enables aspiration of a specific volume followed by a mix sequence and ending with the original aspiration volume in the tips Options Steps Description Edit Aspirate Sets the aspiration volume that is identical to the dispense volume Mix Sets the mixing volume after dispensing Mix Cycles Sets the number of mix cycles Rows Sets the number of rows A row indicator will notify the number of dilutions performed Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Mix Speed Sets speed uniquely for mixing 1 slow 10
57. selection To change a volume select the Edit option and press OK The adjustable volumes are displayed Pipet Mix ma Pipet Mix a 1 TEE ETE Use the Touch Wheel to highlight the volume you Aspirate 250 0pl Pacer tc want to change Aspirate Dispense Mix or Air Gap ees B Press OK and a Volume setting dial is displayed Mor peed s 200 0pl Use the Touch Wheel to change the volume Press Favorites Saveb ASS FmePE OK to confirm your volume selection and gt to save NOTE Use the Arrow Buttons to change the volume in coarse or fine increments Select COARSE with 1 to change the volume in larger increments Select FINE with gt to change the volume in smaller increments The increment sizes vary based on the pipette volume range as shown under 7 5 Pipette Specifications on page 56 Define and select favorite volumes You can define save and select up to ten favorite volumes for quick access These volumes can only be within the pipette volume range There are two ways to access and customize the list of favorite volumes When in Pipet mode use the Touch Wheel to highlight Favorites and press OK When in other modes select the Edit option and press OK The steps with volumes to be adjusted are displayed Use the Touch Wheel to highlight the desired volume and press lt Favorites to display the list of favorite volumes www integra biosciences com 23 VIAFLO I
58. software There are two modes for calibration of VIAFLO II Pipettes Pipette calibration mode for neat transfers and Repeat calibration mode for pipetting aliquots The industry standard is to test and present specifications using neat transfers This is aspirating and dispensing the same volume The Pipette factor of the pipette s software determines the performance of neat transfers and is therefore adjusted in case a pipette performs out of specifications Adjusting a pipette in Repeat calibration mode is normally not required and is not described in this document www integra biosciences com 51 VIAFLO II Pipettes Operating instructions VOO Maintenance The following example is for a 300 ul pipette Select Toolbox on the Main Menu Select the Calibration amp Service and then the Calibration option Press OK Calib amp Service Calibration CCE Calibration com Highlight the Pipette Factor Calibration Reminder Type Factor Service Hist FD Pipette TI Press lt to edit the volume Repeat 1 0000 Help HEdit Saver Calibration D i i i Type Pipette Highlight Target Volume V on the Calibration menu Press OK Use the touch wheel to enter the Target Volume Always use the nominal volume 100 to adjust a VIAFLO II pipette 300 ul in this example Press OK to save your selection Calibration un Type Pipette Move the cursor to the Actual Volume V Use
59. t Count Sets the total number of dispensing steps Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Disp Speed Sets speed uniquely for dispensing 1 slow 10 fast Dispense Sets different volumes for repeated dispensing The maximal 1 Count Count depends on pipette size The total volume is automatically calculated Speed Sets speed of the current pipetting step Pace Sets the time interval between dispenses in repeat pipetting 1 long 9 short Operation Press and release the Run Key to initiate the aspiration of total volume Press and release the Run Key to initiate each subsequent dispense The pipette stops and beeps when ready for the Last Dispense step i e to purge the calculated waste volume amount Alternatively press and hold the Run Key to execute paced dispenses The pipette stops paced dispensing when it reaches the Last Dispense This aliquot contains the accumulated error from all prior dispenses You can choose to use this Last Dispense or discard it During the Last dispense press and hold the Run Key to perform a two step blowout 84 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 8 Variable aspirate mode Application This mode can be used for a variety of collection applications where the aspiration volume is well known This mode is also suited for supernatant collection in microplates Op
60. ters and press 0023456789 OK Once finished press gt to save the name NewD Delete Saver CUSTOM TN The first step is highlighted press OK A Custom program must begin with an Aspirate Mix or Prompt Use the Touch Wheel to select the first step e g Purge Prompt Aspirate and press OK Edit CUSTOM un CUSTOM sun Set the volume value and press OK Aspirate Speed Set the speed for that step and press OK o 100 0yl Coarse Fine 8 After adding the first step the selection should now be on the second line Press OK again to define the second step Continue adding steps until your entire pipetting protocol is defined IMPORTANT NOTE When starting with an Aspirate step followed by a Mix step the tips contain the aspirate volume after completing the last mix cycle When starting with a Mix step the tips are emptied upon completion of the last mixing cycle 38 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Pipetting modes The individual steps based upon the following basic operations Step Description Aspirate Sets the aspiration volume and speed Dispense Sets the dispense volume and speed Mix Sets the mixing volume after dispensing Purge Purges all remaining liquid currently in the GripTip pipette tips A Purge step is automatically int
61. the option indicated with the ee Et E right arrow RUN in the example 2 3 6 Purge button During pipetting you can interrupt the current pipetting protocol and purge all remaining liquid currently in the GripTip pipette tips To do so press Purge 6 Pipet aus The pipette will display a prompt To proceed press and release the Run Key 7 Upon completion Press RUN to of the dispense the first step in the current program will be xs displayed 2 3 7 Run key Press and release the Run Key to initiate aspiration dispense mix purge and special pipetting operations This button is centrally located for left or right handed actuation During dispense you can press and hold the Run Key to perform a two step blowout see 4 3 2 Blowout modes on page 21 12 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Description of the device 2 3 8 Tip ejector The Tip Ejector easily ejects tips from the tip cones The serial number can be found beneath the tip ejector Press and hold the tip ejector in the down position to see the 7 digit serial number 2 3 9 Reset button The Reset Button 13 is located on the back of the VIAFLO II pipettes It is used to reset the RAM in the pipette The programs stored in memory are maintained Once pressed the VIAFLO startup screen will be displayed Press any key to continue and allow the instrument to initialize and home the pi
62. the touch wheel to enter the Actual Volume The actual volume is the mean ofthe weights corrected by the Z factor resulting in V the mean volume in micro liters see section 6 4 7 301 ul in this example Press OK to save your selection Calibration gas Press gt to apply the factor universally to all future Pipet mode motor Piette movements This will correct for any measured inaccuracies observed Repeat 1 0000 in the validation process You are returned to the Calibration menu Press gt to Save your settings Type Factor Edit Save To display the Current and Factory Factors highlight the Pipette Factor on the Calibration menu and press OK A message will indicate that the pipette recalibration is complete To check and revalidate repeat Steps 1 7 in Section 6 4 6 In case a calibration reminder was set it can be reset in the Calibration Reminder menu for either time and or cycles 52 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Maintenance NOTE In case an error message appears when setting the new correction factor follow these steps a Select the Repeat factor in the calibration menu and enter the target and actual measured volume b Press D c Then select Pipet factor and enter target and actual volume d Press gt e Both factors should be the same now f Press Save 6 5 Equipment disposal The VIAFLO II Electronic Pipettes must not be disposed
63. tions Steps Description Edit Count Sets the total number of aspirating steps Asp Speed Sets speed uniquely for aspirating 1 slow 10 fast Disp Speed Sets speed uniquely for dispensing 1 slow 10 fast Aspirate Sets different volumes used for sequentially aspirating in the 1 Count same tip followed by a single dispense The maximal Count depends on pipette size Speed Sets speed of the current pipetting step Operation With the tip s in liquid press and release the Run Key to initiate the first aspiration volume Again in liquid press and release the Run Key to initiate the second aspira tion volume etc Press and hold the Run Key to start Dispense and perform a two step blowout www integra biosciences com 35 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 9 Sample dilute mix mode Application Use this mode to perform sample dilutions where mixing of sample and diluent is required This mode could also be used to introduce and mix diluent and sample to the first column of a serial dilution plate Options Steps Description Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip Air Gap Sets the volume of the air gap to keep both liquids separated Aspirate 2 Sets the volume of the sample in the tip Mix Sets the mixing volume after dispensing Mix Cycles Sets the number of mix cycles Asp Speed Set
64. tting modes The VIAFLO II Electronic Pipettes offers ten predefined pipetting modes Most liquid handling protocols can be easily accommodated using one or more of these modes The options and steps of the different pipetting modes are described in the following subsections 5 2 1 Pipet mode Application Use this mode for quick transfer of liquid to or from microplates Options Steps Description Edit Aspirate Sets the aspiration volume that is equal to the dispense volume Speed Sets speed for the current pipetting step 1 slow 10 fast Favorites Defines up to 10 favorite volumes Operation With the tip s in liquid press and release the Run Key to aspirate With the tip s in the destination plate press and hold the Run Key to execute the dis pense and perform a two step blowout see 4 3 2 Blowout modes on page 21 When the tips are removed from the target plate release the Run Key www integra biosciences com 29 VIAFLO II Pipettes Operating instructions VOO Pipetting modes 5 2 2 Repeat dispense mode Application This mode can be used for fast reagent addition to microplates from one source container You can dispense a large aspirated volume of liquid in multiple aliquots to multiple targets Options Steps Description Edit Dispense Sets the volume for repetitive dispensing The aspirated volume is calculated automatically First A pre dispense volume can be sele
65. verview pipetting modes esseeenene 28 5 2 Detailed description of pipetting modes n se 29 5 2 1 Pipetimiode etn ere Te oes 29 5 2 2 Repeat dispense mode 30 5 2 8 Sample dilute mode sese 31 5 2 4 Pipet MIX miode o ie eterne dis 31 5 2 5 Manual pipet mode sesse e 32 5 2 6 Reverse pipet mode 33 5 2 7 Variable dispense mode sse 34 5 2 8 Variable aspirate mode 35 5 2 9 Sample dilute mix mode 36 5 2 10 Serial dilution mode sss 37 5 3 Custom step based programming mode sesesss 38 5 3 1 Create a custom program 38 5 3 2 Modify existing programs 39 5 3 3 Example of custom mode sssee 40 INTEGRA Biosciences AG VIAFLO II Pipettes Operating instructions VOO Chapter 6 Chapter 7 Chapter 8 Maintenance Gils zGlearihgi siii ette c Daten co Rue Mein iie pea n ege bea 41 6 1 1 Assembly of single channel pipettes 41 6 1 2 Assembly of multichannel pipettes ssss 43 6 2 Sterilization ue te Latet ER e 2A ASS ns 43 6 2 1 Autoclaving the disassembled components 44 6 3 Servicing iion ee bn nah I E RE 44 6 3 1 Shipping to INTEGRA Biosciences esessss 44 6 3 2 Changing O rings of tip fittings 44 6 3 3 bu

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