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VIAFLO Electronic Pipettes Operating instructions

1. sussssse 41 5 3 1 Create a custom program seseeeneee 41 5 8 2 Program tip adjustments in a custom program 42 5 3 8 Modify existing programs 42 5 3 4 Example of custom mode sese 42 INTEGRA Biosciences AG VIAFLO Electronic Pipettes Operating instructions V04 Chapter 6 Chapter 7 Chapter 8 Maintenance 6 1 Gleaning i esee ai foie eae ieee BER 43 6 1 1 Assembly of single channel pipettes 43 6 1 2 Assembly of multichannel fixed spacing pipettes 45 6 2 Ste riliZation ves cates ideft oe ae eret mee 47 6 2 1 Autoclaving the disassembled components 48 6 3 SEVIN iioc Ote eu see tendere abe Dette lal S 48 6 3 1 Shipping to INTEGRA Biosciences sesesss 48 6 3 2 Changing O rings of tip fittings 48 6 3 3 Lubrication uere 49 6 4 Calibration esne ete hot n rede tbe eee deer cidunt 50 6 44 Materials iie RR 50 6 4 2 Definitions eee te tn ree 50 6 4 3 Test Conditions and Environment esses 51 6 4 4 Pre wetting of tips sseseeeeeennenenn 51 6 4 5 Leak tests uci ni ete eed 52 6 4 6 Obtaining the Actual Volume seseeee 53 6 4 7 Calculation of accuracy and precision 54 6 4 8 Adjusting VIAFLO Electronic Pipe
2. Options Steps Description Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip Air Gap Sets the volume of the air gap to keep both liquids separated Aspirate 2 Sets the volume of the sample in the tip Mix Sets the mixing volume after dispensing Mix Cycles Sets the number of mix cycles Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Mix Speed Sets speed uniquely for mixing 1 low 10 fast Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation With the tip s in liquid press and release the Run key to initiate aspiration 1 With the tip s out of the liquid press and release the Run key for the Air Gap Again in liquid press and release the Run key to initiate aspiration 2 Press and release the Run key to dispense the entire tip contents and begin the mix ing routine Upon completing the desired number of mixes a blowout occurs automat ically Remove tips from liquid and press and release the Run key to complete the blowout www integra biosciences com 39 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 10 Serial dilution mode Application Use this mode to perform serial dilutions The Serial Dilution mode enables aspiration of a specific volume followed by a mix sequence and ending with the original aspiration volume in the tips Options Steps Des
3. 1 Write down the ambient temperature and air pressure 2 Perform a pre wet see 6 4 4 3 In the Pipet mode program the high test volume and set pipettor to dispense at speed 8 for 125 ul and 300 ul and speed 6 for 12 5 ul 1250 ul and 5000 ul 4 The first and second dispense to the balance should not be recorded After each dispense re tare the balance 5 Aspirate the Target volume of water keeping the pipette in a vertical to 30 degree position while immersing the pipet tip 2 3 mm below the surface of the water When withdrawing the tip from the liquid gently wipe the tip against the side wall of the vessel to remove any liquid from the outside of the pipet tip 6 Start the first measurement Always pipet directly into the liquid of the weigh container on the balance During dispense keep the run button pressed until the pipette is removed again from the weigh vessel to perform a 2 step blowout Record the weight from the balance 7 After completing the 5 high volume measurements continue with the mid and low test measurements by repeating steps 2 6 www integra biosciences com 53 VIAFLO Pipettes Operating instructions V04 Maintenance 6 4 7 Calculation of accuracy and precision Accuracy The ability of a pipettor to aspirate or dispense the exact volume desired It indicates the proximity of measurement results to the true value Accuracy is also know as systematic error and as the name indicates can be corrected cali
4. 8 Accessories 8 1 Accessories Accessories Charging options and Bluetooth Part No Universal voltage power supply 4200 Lithium ion battery 3 7 V 4205 Single pipettor charging stand 4210 VIALINK update stand 4211 4 pipettor charging stand 4215 Vision Bluetooth module 4220 Bluetooth PC module with PC software 4225 Universal voltage power supply for 4 pipettor charging stand General O ring removal tool 8 2 Consumables O rings for tip fittings 300 ul Replacement kit 24 pack 436 00002 00 Part No 130 00731 00 Part No 100 00027 50 1250 ul Replacement kit 24 pack 100 00028 50 5000 yl Replacement kit 24 pack Lubricant for VIAFLO pipettes and O rings Lube Parker Super O Lube Silicone based 50 g 2 oz for single channel 1250 ul and 5000 ul pipettes 100 00029 50 Part No 100 00135 50 Grease Grease Nye Fluorocarbon Gel 807 5 g 0 2 oz for single channel 12 5 ul 125 ul and 300 ul pipettes 100 00136 50 Reagent Reservoirs Part No 10 ml Disposable reagent reservoirs sterile individually sealed 4331 30 reservoirs per case Disposable reagent reservoirs sterile four sleeves of 4332 50 reservoirs per case Reservoir Base 10 pack 4306 62 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Reagent Reservoirs 25 ml 100 ml 12 5 ul 125 ul 300 yl Accessories Part No D
5. With the tip s in liquid press and release the Run key to aspirate With the tip s in the destination plate press and hold the Run key to execute the dis pense and perform a two step blowout see 4 4 1 Edit option on page 23 When the tips are removed from the target plate release the Run key 32 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 2 Repeat dispense mode Application This mode can be used for fast reagent addition to microplates from one source container You can dispense a large aspirated volume of liquid in multiple aliquots to multiple targets Options Steps Description Edit Dispense Sets the volume for repetitive dispensing The aspirated volume is calculated automatically First A pre dispense volume can be selected independently to Dispense improve accuracy and precision The dispense is discarded Last Dispense A last dispense volume can be selected independently to improve accuracy and precision The dispense is discarded Count The maximum number of dispenses possible count is calculated automatically This count may be reduced to the desired number Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Speed Sets speed of the current pipetting step Pace Sets the time duration between dispenses if keeping Run key pressed Help Help informat
6. or Air Gap Press OK and a Volume setting dial is displayed Use the Touch wheel to change the volume Press OK to confirm your volume selection and gt to save Define and select favorite volumes You can define save and select up to ten favorite volumes for quick access These volumes can only be within the pipette volume range There are two ways to access and customize the list of favorite volumes When in Pipet mode use the Touch wheel to highlight Favorites and press OK www integra biosciences com 23 VIAFLO Pipettes Operating instructions V04 Operation When in other modes select the Edit option and press OK The steps with volumes to be adjusted are displayed Use the Touch wheel to highlight the desired volume and press lt Favorites to display the list of favorite volumes avorite Volumes uL 200 0 pL 175 0 pl Edit Select gt Favorite Volumes Volume 300 0 yL M Favorites 200 0pL 4 200 04 4 COARSE FINE gt 4 4 83 Speed selection The speed option controls the speed at which liquid is aspirated dispensed or mixed in each mode Speed can be set as a value from 1 slowest to 10 fastest see also 7 3 Pipetting speed on page 57 Use the Touch wheel to highlight the desired volume and press gt Select Alternatively modify a volume by pressing 1 Edit Save your setting D When in Run mode use the Touch wheel to highlight the Spe
7. 4 2 Volume selection eese 23 4 43 Speed sSelection citt tette piter 24 4 4 4 PAC i iue ae p dex e ER RA Ue e epe Nequi 25 4 4 5 Count Mix Cycle and Rows sess 25 4 4 6 Helps iint ose etum eed quts 25 4 5 VOYAGER tip spacihg idtm Per pute 26 4 5 1 Set the number of tip positions and the tip spacing 26 4 5 2 Tip spacing operation sse 27 4 5 3 MOMING tNE tips rere teeth ed 27 4 6 Troubleshooting FAQ ssesseeeeeeeeneee nenne 28 AG General huile bat a ea aoaie S Tineo 28 4 6 2 Electronic 5 nne r a SI 29 Pipetting modes 5 1 5 2 5 3 Overview pipetting modes seeeenens 31 Detailed description of pipetting modes eesssss 32 5 24 Pipetimode tee Oe et nde abate iter ees 32 5 2 2 Repeat dispense mode ceeceeeeseeeeneeeeeeneeeeeneeeeeneeeeneees 33 5 2 8 Sample dilute mode esee 34 5 2 4 Pipet mix mode sese nnenn 34 5 2 5 Manual pipet mode esseeeneeeenne 35 5 2 6 Reverse pipet mode sssssseeee 36 5 2 7 Variable dispense mode sseee 37 5 2 8 Variable aspirate mode esse 38 5 2 9 Sample dilute mix mode esee 39 5 2 10 Serial dilution mode sse 40 Custom step based programming mode
8. 4 5 12 5 6 25 5 00 3 20 6 40 3 20 9 0 12 5 2 50 1 60 3 20 1 60 12 4132 50 0 1 12 5 6 00 3 50 25 60 9 60 4 5 125 0 62 5 3 00 1 00 5 12 1 92 9 0 125 2 00 0 70 2 56 0 96 The multichannel and VOYAGER pipettes specifications represent the performance of all channels of the pipettes Precision Coefficient of Variation 60 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Technical Data 7 5 Z Correction Factors Temp Air Pressure kPa C 80 85 90 95 100 101 3 105 18 0 1 0022 1 0023 1 0023 1 0024 1 0025 1 0025 1 0025 18 5 1 0023 1 0024 1 0024 1 0025 1 0025 1 0026 1 0026 19 0 1 0024 1 0025 1 0025 1 0026 1 0026 1 0027 1 0027 19 5 1 0025 1 0026 1 0026 1 0027 1 0027 1 0028 1 0028 20 0 1 0026 1 0027 1 0027 1 0028 1 0028 1 0029 1 0029 20 5 1 0027 1 0028 1 0028 1 0029 1 0029 1 0030 1 0030 21 0 1 0028 1 0029 1 0029 1 0030 1 0031 1 0031 1 0031 21 5 1 0030 1 0030 1 0031 1 0031 1 0032 1 0032 1 0032 22 0 1 0031 1 0031 1 0032 1 0032 1 0033 1 0033 1 0033 22 5 1 0032 1 0032 1 0033 1 0033 1 0034 1 0034 1 0034 23 0 1 0033 1 0033 1 0034 1 0034 1 0035 1 0035 1 0036 23 5 1 0034 1 0035 1 0035 1 0036 1 0036 1 0036 1 0037 24 0 1 0035 1 0036 1 0036 1 0037 1 0037 1 0038 1 0038 24 5 1 0037 1 0037 1 0038 1 0038 1 0039 1 0039 1 0039 Z values in microliters per milligram www integra biosciences com 61 VIAFLO Pipettes Operating instructions V04
9. a two step blowout www integra biosciences com 37 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 8 Variable aspirate mode Application This mode can be used for a variety of collection applications where the aspiration volume is well known This mode is also suited for supernatant collection in microplates Options Steps Description Edit Count Sets the total number of aspirating steps Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Aspirate 1 5 Sets upto 5 different volumes used for sequentially aspirating in the same tip followed by a single dispense Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation With the tip s in liquid press and release the Run key to initiate the first aspiration volume Again in liquid press and release the Run key to initiate the second aspira tion volume etc Press and hold the Run key to start Dispense and perform a two step blowout 38 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 9 Sample dilute mix mode Application Use this mode to perform sample dilutions where mixing of sample and diluent is required This mode could also be used to introduce and mix diluent and sample to the first column of a serial dilution plate
10. aspiration and dispense steps and can view the display to confirm how much liquid has been aspirated or dispensed Manual control over the dispense steps is perfect for performing Titrations or for controlling the loading of samples in gel lanes Options Steps Description Edit Aspirate Sets the aspiration or dispensing volume Toggle between Aspirate and Dispense using the Direction menu option Speed Sets speed of the current pipetting step 1 low 10 fast Direction Changes the direction of pipetting between aspiration and dispensing Favorites Defines up to 10 favorite volumes Help Help information is available for each mode Operation When aspirating the motor will stop when you release the Run key or when the pro grammed aspirate volume is reached You can change pipetting direction at any time even if aspiration volume is not reached Change the direction of pipetting by pressing OK on the Direction option The notation on the display changes between A Aspirate and V Dispense Titrations can be performed by dispensing in this mode The volume remaining in the tip s is always actively displayed NOTE Use slower pipetting speeds 1 5 for better control and resolution www integra biosciences com 35 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 0 Reverse pipet mode Application Use this mode when performing reagent addition where a blowout is not desired whe
11. gt to select the option indicated with the Reverse Pipet V lt JHELP RUND right arrow RUN in the example For Voyager models these buttons are also used for tip spacing adjustments 2 3 6 Purge button During pipetting you can interrupt the current pipetting protocol and purge all remaining liquid currently in the GripTips To do so press Purge 6 Run Pipet Anais The pipette will display a prompt PressRun U To proceed press and release the Run key 7 Upon completion of toPurge the dispense the first step in the current program will be displayed 2 3 7 Run key Press and release the Run key to initiate aspiration dispense mix purge and special pipetting operations This button is centrally located for left or right handed actuation During dispense you can press and hold the Run key to perform a two step blowout see 4 4 1 Edit option on page 23 12 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Description of the device 2 3 8 Tip ejector The tip ejector easily ejects tips from the tip cones The serial number can be found beneath the tip ejector Press and hold the tip ejector in the down position to see the 7 digit serial number 2 3 9 Reset button The reset button 13 is located on the back of the VIAFLO pipettes It is used to reset the RAM in the pipette The programs stored in memory are maintained Once pressed the VIAFLO startup screen will be
12. moves the tip spacing selection in a circular loop This process can be executed at any step in the program 4 5 3 Homing the tips Homing the tips may be necessary if the tip spacing motor was restricted from achieving its proper position Every time the pipette is turned on the tip spacing motor homes and returns to the position that it was last in Ip Spacing Select Step Positions E First 9 0 mm Middle 11 5 mm Last 14 0 mm Spacing 9 0 mm You also may home the tip spacing motor as needed To do so press 1 on the Tip Spacing menu to select the Home Tips option www integra biosciences com 27 VIAFLO Pipettes Operating instructions V04 4 6 Troubleshooting FAQ 4 6 1 General Problem Pipette leaks or does not fill with Probable cause O ring was not properly installed Operation Remedy Replace the tip fitting O ring see 6 3 2 liquid after During reassembly the O ring reassembly was damaged Leakage Tip incorrectly attached Attach a new tip Foreign particles between tip and tip cone Foreign particles between the piston the O ring and the cylinder Damaged red O ring Clean tip cones Attach new tips Change the red O ring see If leak persists contact service Dispense results are inaccurate Unsuitable calibration Incorrect aspirate and dis pense speed Recalibrate with the liquids in question Adjust aspirati
13. represent the performance of all channels of the pipettes Precision Coefficient of Variation www integra biosciences com 59 VIAFLO Pipettes Operating instructions V04 Technical Data VOYAGER Tip Spacing Pipettes ISO8655 limits Chan Part Volume poni Test Accu Precision ACCU precision T P nel No Range ments Volume racy 5 racy lt Spacing pl ul p 196 mm 4 4143 10 0 0 5 30 5 00 2 00 26 67 10 00 9 0 300 0 150 2 75 1 00 5 33 2 00 32 5 300 1 80 0 50 2 67 1 00 4 4144 50 0 1 125 6 00 1 50 12 80 4 80 9 0 1250 0 625 3 00 0 75 2 56 0 96 32 5 1250 1 50 0 50 1 28 0 48 6 4163 40 0 0 5 30 5 00 2 00 26 67 10 00 9 0 300 0 150 2 75 1 00 5 33 2 00 19 5 300 1 80 0 50 2 67 1 00 6 4164 50 0 1 125 6 00 1 50 12 80 4 80 9 0 1250 0 625 3 00 0 75 2 56 0 96 19 5 1250 1 50 0 50 1 28 0 48 8 4121 0 5 0 01 1 25 15 00 10 00 32 00 16 00 4 5 12 5 6 25 5 00 3 20 6 40 3 20 14 0 12 5 2 50 1 60 3 20 1 60 8 4122 5 0 0 1 12 5 6 00 3 50 25 60 9 60 4 5 125 0 62 5 3 00 1 00 5 12 1 92 14 0 125 2 00 0 70 2 56 0 96 8 4123 10 0 0 5 30 5 00 2 00 26 67 10 00 9 0 300 0 150 2 75 1 00 5 33 2 00 14 0 300 1 80 0 50 2 67 1 00 8 4124 50 0 1 125 6 00 1 50 12 80 4 80 9 0 1250 0 625 3 00 0 75 2 56 0 96 14 0 1250 1 50 0 50 1 28 0 48 12 4131 0 5 0 01 1 25 15 00 10 00 32 00 16 00
14. turn fully on when the line cord is connected The current screen remains displayed If the pipette is idle while charging the display may dim see 4 1 Turn on off the device on page 20 but it will continue to show the battery life indicator When the display shut off time is reached the instrument will shut off 3 3 Toolbox adapt your VIAFLO Electronic Pipettes The Toolbox provides options to adapt the device to appropriate applications setting personal preferences calibration computer connectivity and storing owner information Toolbox mode Description Preferences Customizes the system parameters Calibration amp Service Sets calibration and service history options Communications Enables communication between your VIAFLO Electronic Pipettes and a PC Owner Information View your pipettor s serial number and set a personal ID Help Language Sets language specific help Write Protect Protects programs or menu options from modification Password Key Displays the encrypted password www integra biosciences com 15 VIAFLO Pipettes Operating instructions V04 Installation 3 3 4 Preferences Preferences customizes your system parameters Select a preference and press OK to access Preference Description Range Sound Simple tones indicate completion of operations and errors Select an option and press OK to change the On Off status of the beep tone between On and Off
15. 00 ul or 1250 ul Slide the O Ring removal tool sidewards onto the tip fitting until the O ring a builds a loop Cut the O ring with a fine scissor and remove it For 5000 ul pipettes use forceps to remove the O ring Slide a new O ring over the tip fitting b The internal seals and O rings are subject of very slow wear out An undamaged thin lubricant film is important to keep the seals tight The lubricant recommended depends on the VIAFLO pipette size see 8 2 Consumables on page 62 c b a lo Single channel 12 5 pl 125 pl 300 yl Use Fluorocarbon Gel 100 00136 50 to lightly lubricate the piston a without components slided onto Put a drop of grease onto your fingers lubricate the black O ring b at slide it over the white seal c Single channel 1250 pl and 5000 pl Use the Super O Lupe 100 00135 50 to lightly lubricate the outer ring of the Cup Seals d e For the 5000 ul pipette also lubricate the bottom of the white Cup Seal www integra biosciences com 49 VIAFLO Pipettes Operating instructions V04 Maintenance 6 4 Calibration The VIAFLO Pipettes are factory tested and calibrated under environmentally controlled conditions using a gravimetric procedure in accordance with ISO 8655 standards For the accuracy and precision specifications see 7 4 Pipette Specifications on page 58 Several conditions that would require recalibration of your pipette include a desire t
16. 60 0 15 0 80 0 30 8 4021 0 5 12 5 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 8 4022 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 8 4023 10 0 0 5 30 4 00 1 20 26 67 10 00 300 0 150 2 00 0 60 5 33 2 00 300 1 60 0 35 2 67 1 00 8 4024 50 0 1 125 6 00 1 10 25 60 9 60 1250 0 625 2 40 0 50 5 12 1 92 1250 1 20 0 30 2 56 0 96 58 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Technical Data VIAFLO Electronic Pipettes ISO8655 limits Volume Chan Part Moe Incre ee Accuracy Precision Accuracy Precision nel No ments 496 ul ul ul 12 4031 0 5 12 5 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 12 4032 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 12 4033 10 0 0 5 30 4 00 1 20 26 67 10 00 300 0 150 2 00 0 60 5 33 2 00 300 1 60 0 35 2 67 1 00 12 4034 50 0 1 125 6 00 1 10 25 60 9 60 1250 0 625 2 40 0 50 5 12 1 92 1250 1 20 0 30 2 56 0 96 16 4041 ge 495 0 01 1 25 10 00 6 00 32 00 16 00 6 25 4 00 1 60 6 40 3 20 12 5 2 00 0 80 3 20 1 60 16 4042 5 0 0 1 12 5 3 75 1 50 25 60 9 60 125 0 62 5 2 50 0 70 5 12 1 92 125 1 60 0 35 2 56 0 96 The multichannel and VOYAGER pipettes specifications
17. 9 7 BUD KOya erret asp dete erede niet iig busbee es Pues mu aie de 12 2 3 8 Tip eJector uiui RUE tee leri tees 13 2 3 0 Reset button oo nte uino is 13 Installation 3 1 Operating environment sssssseeeeeenneeeee 14 3 2 Charging the battery eese 14 3 2 1 Charging the battery on a stand 14 3 2 2 Charging the battery with UPS 15 3 3 Toolbox adapt your VIAFLO Electronic Pipettes 15 3 9 14 Preferences iiie ie PO tM 16 3 3 2 Calibration amp Services sssssseee 17 3 3 9 GOMMUNICATIONS ise ii nce e Ete RE Hi pags 18 3 3 4 Owner Information sese 18 3 3 5 Help Language mente 19 3 9 0 Write protect s bee ott eG bee re c eate 19 3 9 7 Password key e tee ee eerie 19 www integra biosciences com 3 VIAFLO Electronic Pipettes Operating instructions V04 Chapter 4 Chapter 5 Operation 4 1 Turn on off the eViCG eee eeeeseeteneeeeeeeeaeeeseeeaeseaeetaeeeaeeeeetaas 20 4 2 Attaching and removing GripTips eeeeeeeeee 20 4 3 Start pipetting 2 caede te duse coe dnce deus 21 4 34 PIpetlilig scuto teas 21 4 3 2 Blowout M deSasi nnn inon aaa ie 21 4 3 8 Recommendations for pipetting sseseeesss 22 4 4 Pipetting options and settings 0 ee eeeseeeeneeeesneeeeeneeeeeneeersaeeee 23 4 4 T Et ODIODE cuneo treten et cepe heirs 23 4
18. GRA Biosciences is responsible for return shipping costs when product is within the warranty period Use original packaging when shipping if possible A WARNING A If working with infectious materials e g human pathogens VIAFLO pipettes need to be decontaminated before sending them to service and the declaration on the absence of health hazards must be signed This is necessary to protect service personnel Please call fax or use INTEGRA Biosciences s online RMA page for a return authorization number and Decontamination Document before returning any product 6 3 2 Changing O rings of tip fittings 300 ul 1250 ul and 5000 ul VIAFLO pipettes feature tip fittings with red O rings This O ring is used to seal against the inside wall of GripTips and serve to reduce the required tip loading and ejecting forces O rings are made of durable silicone If necessary e g in case of a leakage due to damaged O ring you can replace these O rings A set of spare O rings and an O ring removal tool are included with the large volume multi channel pipettes and can be ordered separately see 8 Accessories on page 62 480 SNTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance E e f 6 3 3 Lubrication Lo WARNING Avoid mechanical damage of the tip fittings For 300 ul or 1250 ul pipettes choose the side of the O ring removal tool corresponding to the size of the pipetting head 3
19. INTEGRA VIAFLO Electronic Pipettes Operating instructions 900 00002 00_V04 INTEGRA Declaration of conformity Konformitatserklarung C D claration de conformit Declaraci n de conformidad Dichiarazione di conformit INTEGRA Biosciences 2 Wentworth Drive Hudson New Hampshire 03051 declares on its own responsibility that the product erklart in alleiniger Verantwortung dass das Produkt d clare sous sa responsabilit exclusive que le produit declara bajo su propia responsabilidad que el producto dichiara sotto la propria responsabilita che il prodotto VIAFLO Electronic Models 4011 4012 4013 4014 4015 4021 4022 4023 Pipettes 4024 4031 4032 4033 4034 4041 4042 4121 4122 4123 4124 4131 4132 4143 4144 4163 4164 in accordance with EC directives gemass der EU Richtlinien est conforme au terme de la directives CE de acuerdo con las directivas CE in conformita alle direttive CE 2006 95 EC Low voltage equipment 2004 108 EC Electromagnetic compatibility 2002 95 EC Restriction of Hazardous Substances 2002 96 EC Waste Electrical and Electronic Equipment is in compliance with the following normative documents mit den folgenden normativen Dokumenten bereinstimmt aux documents normatifs ci apr s cumple las documentos normativos soddisfa le normative seguenti EN 61010 1 Safety requirements for electrical equipment for measurement contro
20. Step complete at the end of a program step Program complete at program completion Purge key when Purge is pressed Error message when an error message appears or when illegal data entry is attempted Spinner When spinning the Touch wheel Last dispense before the last dispense in Repeat Dispense and Variable Dispense Display Customizes your display Press OK to select an option use the Touch wheel to display the desired value Start up display Select the startup display at one of VIAFLO Logo the following VIAFLO Logo None or Custom up to None two uploaded with VIALINK Press gt to Save your Custom 1 or 2 selection Display dim time The display will dim after a set num Never ber of minutes A shorter dim time helps preserve bat 1 20 min tery life Press OK to save your selection 5 20 min Turn off time By default the pipette will turn off after 5 1 hour minutes of idle time A shorter turn off time helps pre serve battery life You can change this setting Press OK to save your selection Main Menu Select a function to be hidden from the main menu Off and press OK e g Tip Spacing Pipet Repeat Dispense Dilute Pipet Mix On Off Spinner Adjust your Touch wheel spin sensitivity Low Medium High Pipetting Select an option and press OK Purge key speed Choose the desired purge speed 1 10 and press OK to save your selection Delayed blowin Choose a timed delay between the blowout
21. a Prompt use the Touch wheel to select one of 3 lines and press OK Highlight a character that you want to use and press OK You can press 1 to Delete the last character entered After you enter the desired text press to Save To save and store a Custom program press D www integra biosciences com 41 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 3 2 Program tip adjustments in a custom program From the Main Menu select Custom and press lt to create a new program Edit Custom 1 Edit Custom Set Spacing Scroll down to highlight Tip Spacing and press OK 90m 115 140mm Either select one of the three favorite Tip Spacings by Spacing mm using the touch wheel or press lt and D to set the 9 0 desired Tip spacing Select Operation Close Open gt 5 3 3 Modify existing programs Custom T m At the Custom program display use the Touch wheel to highlight an Lion ci oon existing program Press the OK button to select it Select an option View Edit View Edit Delete Copy Name to modify the program rmm Custom 1 RUNG Press Back to return to the list of Custom programs To run the program press D 5 3 4 Example of custom mode Application The task is to combine 2 different liquids in a 96 well plate for a kinetic assay and then mixing it to achieve a homogenous solution The custom program would be set up as followed Program step Action 1 Aspi
22. and the blowin delay before the piston homes at the end of a dispense if no two step blowout is performed see 4 3 2 Blowout modes on page 21 0 0 5 1 1 5 s After changing desired settings press gt to Save 16 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 3 3 2 Calibration amp Services These options enable you to set calibration features review service history and move the pipettor head into the parking position Calibration amp Services Description Installation Range Calibration Allows for re calibration of the VIAFLO Electronic Pipettes to restore accuracy The calibration factors for Pipet and Repeat type are displayed To edit the calibration volumes press 1 Target Volume This is the volume you are interested in using for the calibration Actual Volume This is the measured volume obtained when dispensing the target volume Factory Reset Resets the correction factor back to the original factory setting Press to apply the factory setting Current Factor Displays the factor currently in use Calibration Reminder Time or Cycles Sets a calibration reminder based on a specified time frame or number of pipetting cycles When the calibration reminder is displayed press any key to confirm However the reminder will reappear every time the pipettor is turned on until you change the reminder time or use the reset option T
23. brated Precision Coefficient of variation CV Precision indicates the repeatability or reproducibility of the measurement It is also called random error and is therefore an unpredictable error which can not be corrected calibrated Target V Actual V The following symbols are used throughout this text Selected test or target Volume Accuracy Measured Liquid Weight g Z factor see 7 5 Actual mean Volume ml or ul V m Z V n Number of measurements Precision Conversion of the mass to volume The values obtained by balance readings are in grams These values need to be converted to micro liters using the Z correction factor It takes into account the water density and air buoyancy during weighing at the corresponding test temperature To determine the correct Z factor find the intersection between temperature and air pressure in Table 7 5 Round up temperature and air pressure values Add together the weights n e g n 5 obtained in 6 4 6 divide the sum by n to calculate the mean weight and then multiply it by the corresponding Z factor Mean volume V in milliliters or micro liters which is the Actual Volume 1 n V es gt Vi xZ i l Calculation of systematic error Accuracy The systematic error e can be calculated using the following equation with V being the selected test volume B4 0 SNTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance or in perce
24. ce 16 Battery 2 3 8 Display The Display shows all pipetting options Main Menu p Battery life indicator gt Repeat Dispense Sample Dilute Pipet Mix Manual Pipet Reverse Pipet V OK xiHELP RUND Current mode Instruction Vc d __ gt Volumes Press RUN to go Aspirate 250 uL Dispense 250 pL Active step SSS Edit Sr Speed 8 Options pe www integra biosciences com 11 VIAFLO Pipettes Operating instructions V04 Description of the device 2 3 4 Touch wheel The Touch wheel is fully operational with only one hand Rotational finger movements translate into up and down cursor movement on the display The Touch wheel is fully functional with the use of latex gloves V a Move finger on the Touch wheel to choose and olume olume e p H H Volume DEI ra acer highlight an option on the display Press OK 4 to Volume3 200 0 pL 200 0yL make the selection Volume4 175 0 uL Volume5 150 0 uL 200 0 When a setting dial is displayed spin the Touch wheel Volume 1250 pV to change the value and press OK Edit Seleci 4 COARSE FINED 2 3 5 Leftand right arrow buttons Manen At times you will see lt and gt on a display screen These buttons Repeat Dispense are used to select options Sample Dilute A mi Pipet Mix i Press lt to select the option indicated with the left arrow HELP in Manual Pipet the example beside Press
25. cription Edit Aspirate Sets the aspiration volume that is identical to the dispense volume Mix Sets the mixing volume after dispensing Mix Cycles Sets the number of mix cycles Columns Sets the number of columns A column indicator will notify the number of dilutions performed Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Mix Speed Sets speed uniquely for mixing 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation With the tip s in liquid press and release the Run key to initiate the aspiration of the reagent Submerge the GripTips in the sample located in the first column Press the Run key to activate the dispense The mix sequence will automatically initiate following the dis pense After the mix steps position the GripTips in the second column and press the Run key to start the second dilution Columns first number and Mix Cycles second number are tracked on the display Mix Cycles are shown in red when mixing A green dot on the column number indi cates the active program step 40 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 3 Custom step based programming mode Application Use the Custom program mode to create personalized pipetting tasks Up to twenty programs can be stored 5 3 1 Create a cust
26. d To eliminate this error simply discard the first volume after the aspiration step It is possible to preprogram a smaller first volume to be discarded Program a last dispense volume that is discarded To properly dispense liquids ensure that the pipette tip is at a 0 30 angle against the wall of the container or well After dispense touch tip against wall in order to rid tip of residual liquid Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting Calibrate based on fluid type All Vision and Voyager Pipettes are tested and cali brated at the factory for use with distilled water at room temperature It may be neces sary to re calibrate your pipette if the liquid to be used has different physical properties specific gravity and vapor pressure than water Calibration mode can be accessed in the Toolbox menu When using factory calibration the liquid to be pipetted should not contain specific gravity and vapor pressure that is significantly different from distilled water For pipetting liquids with high vapor pressures such as alcohol methanol or ethanol use relatively fast pipetting speeds and avoid prolonged pauses after aspiration i WARNING A Avoid pipetting for extended periods To minimize the risk of repetitive strain injury include pauses of several minutes 22 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Operation 4 4 4 4 1 The E
27. d release the Run key to initiate each aspiration remove tips from liquid for air gap aspiration Press and hold the Run key to perform a two step blowout The entire tip contents will be dispensed together 5 2 4 Pipet mix mode Application Use this mode when mixing is required immediately after transfer of liquid This mode saves a programming step by incorporating the mix option after dispensing Options Steps Description Edit Aspirate Sets the aspiration volume that is equal to the dispense volume Mix Sets the mixing volume after dispensing Mix Cycles Sets the number of mix cycles Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Mix Speed Sets speed uniquely for mixing 1 low 10 fast Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation Press and release the Run key to initiate the aspiration Press and release the Run key to dispense Mixing occurs automatically after the dis pense step Upon completing the desired number of mixes a blowout is initiated automatically prompting you to remove the tip s from the liquid and press Run to complete the blowout 934 11 1 SNTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 5 Manual pipet mode Application This mode can be used when the aspiration volume is not defined or unknown You have control over the
28. displayed Press any key to continue and allow the instrument to initialize and home the pipette The routine ends by displaying the Main Menu www integra biosciences com 13 VIAFLO Pipettes Operating instructions V04 Installation 3 Installation 3 1 Operating environment The VIAFLO Electronic Pipettes have been designed for use in a laboratory They shall be operated in a dry and dust free location with a temperature of 5 40 C and a maximal non condensing relative humidity of 80 96 3 2 Charging the battery All VIAFLO hand held pipettes share the same rechargeable long life lithium ion battery that has a capacity of 1050 mAmp hours The battery should be charged for at least one hour before pipetting A full charge takes 2 5 hours and will provide approximately 3500 pipetting cycles A battery indicator is provided on the pipette display 2 3 3 indicating different states Blinking red icon Low battery A recharging message will appear Blinking grey icon Pipette is recharging Blinking green icon Pipette is recharging and battery is 90 charged Solid green icon Battery is fully charged and the pipette is still connected to the mains adapter Three static grey bars Battery is fully charged CAUTION Use only the approved INTEGRA battery power supply or charging stand Use of an incompatible power transformer can damage the pipette Battery charging can take place in two ways using a pipette chargi
29. dit option is available for each mode It enables you to access the variables that you can adjust for a pipetting mode These variables include Speed Volume Pace Count Mix Cycles Rows and Direction Additional steps include First dispense Last dispense Pipetting options and settings Edit option Air gap Aspirate speed Dispense speed etc Run Repeat Disp Press RUN to go Aspirate 1250 pL Dispense 100 pL Speed 8 Pace 8 v Edit Repeat Disp Select Step Dispense FirstDispense OyL LastDispense 5OpL Count 12 Asp Speed 8 V Favorites Save 4 4 2 Volume selection Select a pipetting mode Then select Edit on the list of options and press OK A list of associated steps is displayed For example if selecting Edit on the Repeat Dispense screen the modifiable steps associated with Repeat Dispense are displayed To change a volume select the Edit option and press OK The adjustable volumes are displayed dit Pipet Mix Select Step 500pL XlFavorites dit Pipet Mix NOTE Use the navigation buttons to change the volume in coarse or fine increments Select COARSE with 1 to change the volume in larger increments Select FINE with to change the volume in smaller increments The increment sizes vary based on the pipette volume range as shown under 7 4 Pipette Specifications on page 58 Use the Touch wheel to highlight the volume you wantto change Aspirate Dispense Mix
30. during reset trollable on Sensitivity setting is not set Adjust the spinner sensitivity with correctly Toolbox Preferences Spinner see 3 3 1 Displayed charac Unknown Reset the pipette ters are scrambled Battery charging e Charge stand pins are out of Check that both charge stand indicator is not pul sing while on the stand Unit does not turn on when placed on the charging stand place conductor pins are at the same height Make sure the charger is plugged in Indicates too much friction was encountered during operation Error message is displayed Home Found or Home Not Found ure www integra biosciences com Indicates possible motor fail Reset the pipette If problem persists please con tact service for technical assis tance 29 VIAFLO Pipettes Operating instructions V04 Operation Problem Probable cause Remedy Motor sounds rough Pipette has been set to the e Verify the speed setting The and aspiration is slowest speed slowest speed setting normally very slow causes the motor to run slowly and louder than normal 30 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 Pipetting modes This chapter describes how to program the VIAFLO Electronic Pipettes in two ways Function based pipetting modes You can select from ten predefined pipetting modes that you can quickly and easily edit and e
31. e is higher than the volume to be dispensed Variable Dispense Allows dispensing multiple aliquots of different volumes Variable Aspirate Allows aspirating multiple aliquots of different volumes Sample Dilute Mix Allows aspirating two liquids separated by an air gap followed by a complete dispense and Mix step Serial Dilution Allows aspirating a transfer volume followed by a mix Columns and Mix Cycles are tracked on the display Custom Allows to create and store of up to 20 multi stepped pipetting protocols Press the OK to access the pipetting mode and to start defining parameters www integra biosciences com 31 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 Detailed description of pipetting modes The VIAFLO Electronic Pipettes offers ten predefined pipetting modes Most liquid handling protocols can be easily accommodated using one or more of these modes The options and steps of the different pipetting modes are described in the following subsections 5 2 1 Pipet mode Application Use this mode for quick transfer of liquid to or from microplates Options Steps Description Edit Aspirate Sets the aspiration volume that is equal to the dispense volume Speed Sets speed for the current pipetting step 1 low 10 fast Favorites Defines up to 10 favorite volumes Help Help information is available for each mode Operation
32. eable battery located inside the pipette Li ion 3 7 V 1050 mAh Bag of spare O rings 300 and 1250 ul versions only Accuracy and precision certificate CAUTION Verify the scope of delivery when unpacking the device and check for potential transportation damage Do not operate a device that is damaged instead contact your local dealer 2 2 Pipette configurations VIAFLO single and multichannel pipettes couple an easy to use touch wheel controller with intuitive programming They are microprocessor controlled and stepper motor driven In addition the VIAFLO VOYAGER adjustable tip spacing pipettes provides you with the ability to vary tip spacing www integra biosciences com 9 VIAFLO Pipettes Operating instructions V04 Description of the device 2 3 Overview of the VIAFLO Electronic Pipettes 2 3 1 VIAFLO pipette parts 10 oO Oo ON OO FR WO YP Display Back button to navigate backward Touch wheel spin to scroll and move the cursor OK button to make a selection Left and right arrow buttons for selections Purge button to empty tips Run key to start operations Tip ejector Finger hook facilitates easy operation Volume indicator label color matches GripTip rack insert Ejector sleeve Tri lobe tip interface INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Description of the device 2 3 2 Back view 13 Reset button 13 14 14 Power connector oe 15 Charging stand interfa
33. ed option and press OK Select the speed and press OK to save your setting Run Pipet CA Run Pipet Press RUN to go Press RUN to go Aspirate 2504L Aspirate 250 uL Dispense 250jL Dispense 250 pL Edit Speed Favorites 8 Run Dilute Edit Dilute dit Dilute Press RUN to go Select Step Select Step Aspirate 1 7504L Aspirate 1 Aspirate 1 750pL Air Gap 50L Air Gap SOL Air Gap SOL 5 Aspirate 150pL Asp Speed 8 Speed 8 Disp Speed 8 Help XlFavorites Save Speed may be changed in most Edit menus Scroll to the Speed and press OK Choose the speed press OK and press gt to save your selection The speeds selected in each mode i e Pipet Repeat Dispense etc are stored for that mode only Speeds can be set independently for each operation Aspirate Dispense Mix NOTE Viscous samples should be aspirated and dispensed at the slowest speeds to ensure accurate pipetting To dispense liquids with low viscosity and high vapor pressure such as alcohol use relative fast pipetting speeds and avoid prolonged pauses for aspiration 24 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Operation 4 4 4 Pace The Pace option sets the time gap between dispenses in repeat pipetting Pace is used in the Repeat Dispense and Variable Dispense modes While you press and hold the Run key the pipettor will dispense multiple programmed volumes with the selec
34. elp option from Pipet Mix m the list and press OK Manual Pipet Speed 8 A Reverse Pipet y Favorites HELP RUN Help www integra biosciences com 25 VIAFLO Pipettes Operating instructions V04 Operation 4 5 VOYAGER tip spacing The VOYAGER multichannel pipettes provides you with the ability to vary tip spacing The tip spacing is expressed in millimeters and represents the distance between adjacent tips This spacing can be adjusted in either 0 1 mm or 0 25 mm increments depending on the instrument selected NOTE Tip spacing is only displayed if Tip Spacing is switched on under Toolbox Preferences Main Menu 4 5 1 Set the number of tip positions and the tip spacing It is possible to set two or three tip spacing values based on labware types to be used Main Menu Tip Spacing Use the touch wheel to highlight Tip Spacing on the Select Stee Main Menu Press OK The tip spacing menu Pipet Positions I displays Repeat Dispense First 9 0 mm Sample Dilute Midde 11 5mm The number of positions the VOYAGER will move Pipet Mix Last 14 0 mm Manual Pipet V Spacing 9 0 mm between 2 or 3 4 SHEP RUNS Home Tps Save gt The First Middle and Last positions The current spacing that the tips are in To change the number of positions to move between use the touch wheel to scroll to Positions and highlight either 2 or 3 Press OK to toggle between 2 or 3 positions Press t
35. for lint or dust particles It is recommended to replace the O Ring black and Seal white Assembly e before reassembly The O Ring and Seal slide on off the piston and can be replaced easily 1 Lightly lubricate the piston and seals see 6 3 3 Lubrication on page 49 Position the Piston b at the small magnet to connect the Piston to the upper portion of the pipette 2 Slide the Seal Retainer c onto the Piston and up into the pipette The end of the Seal Retainer that has an extended edge lip should be closest to the upper portion of the pipette over the magnet 3 Slide the Seal Retainer Spring d onto the Piston It will rest against the Seal Retainer 4 Slide the lightly lubricated Seal white and O Ring black Assembly c onto the Piston The white Seal will be closest to the Seal Retainer Spring Be sure the black O Ring is securely in place on the white Seal 5 With the Ejector Sleeve h in hand slide the Ejector Sleeve Spring g onto the top of the Sleeve 6 Slide the Ejector Sleeve into the Cylinder Assembly a 7 Position the black Sealing Ring f over the Ejector Sleeve h at the top of the Cylinder Assembly Turn clockwise until the Sealing Ring snaps into place and secures the Ejector Sleeve in the Cylinder Assembly 8 Slide the Cylinder Assembly over the Piston 44 0000000000000 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance 9 Screw counterclockwise t
36. fter homing the menu last used is displayed Press the Back button 2 as many times as necessary to return to the Main menu Turn off To turn off the pipette press and hold the Back button 2 for 3 seconds NOTE The VIAFLO Electronic Pipettes will dim and turn off automatically after a preset duration of inactivity This duration is 5 minutes by default and configurable with the Toolbox see 3 3 1 Preferences on page 16 42 Attaching and removing GripTips CAUTION To ensure optimal performance of your VIAFLO Electronic Pipettes always use GripTips which have been designated for the use with the VIAFLO Electronic Pipettes see 8 2 Consumables on page 62 The unique tri lobe design of the VIAFLO Electronic Pipettes reduces attachment and ejection forces ensures a perfect fitting to prevent the tips from falling off and to provide a perfect sealing On a multichannel pipette all tips sit on the same height Tri lobes minimize contact surface and temporarily deforms the tip shaft Shoulder provides a positive stop to prevent over tightening and all tips seal at the same height O ring provides forgiving and robust seal surface for the pipet tip 20 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Operation Attach the tips When loading tip s press the pipette into the appropriate GripTip s until you hear and feel a click indicating that a seal has been achieved Once you feel the c
37. he pipette to aspirate full volume Set aspirating speed at 6 Aspirate by pressing the Run key 4 Dispense Keep the Run key pressed until all liquid is expelled remove the tip from the liquid and then release the Run key to perform a two step blowout see 4 3 2 Blowout modes on page 21 www integra biosciences com 51 VIAFLO Pipettes Operating instructions V04 Maintenance 6 4 5 Leak test It is recommended to perform a leak test every 3 months or when errors occur 1 Prewet tips as described above 2 Inthe Pipet Mix mode program the VIAFLO Electronic Pipettes to aspirate full volume at speed 6 and mix at full volume Set to mix to 6 cycles and set mixing speed at 6 3 Aspirate full volume and verify liquid level is not decreasing during 2 3 seconds while GripTips are still in distilled water 4 Remove the GripTips from the liquid and hold the pipette at a 30 angle Wait for 20 seconds a Observe whether liquid droplets are forming at the end of the GripTips b On a multichannel pipette verify liquid level is even across all channels 5 Immerse GripTips approx 2 mm into distilled water and press Run key to start mix cycle a Observe whether air bubbles are forming when dispensing b On a multichannel pipette check whether the levels stay approximately on the same level across all channels c At the end of a last dispense it is normal to have air bubbles because a blow out is performed For a VOYAGER pipette car
38. imer Press OK to turn the reminder timer On or Off Remind in Use the Touch wheel to set a reminder interval for calibration time in months or in thousands of cycles Reset Resets the timer to the defined calibration inter val With this option highlighted press OK and gt Save to enable On Off 1 12 months or 10k 240k cycles Service His tory Displays notes of any service that took place on the VIAFLO Electronic Pipettes listed newest entry first Calibrate Tip Spacing Starts calibration of tip spacing of VOYAGER pipettes After changing desired settings press gt to Save www integra biosciences com 17 VIAFLO Pipettes Operating instructions V04 3 3 3 Communications The VIAFLO Electronic Pipettes can be programmed from a PC via a Single Pipette Programming Stand 4211 or via wireless Bluetooth connection For download of VIALINK software and further information refer to VIALINK operating instructions on www integra biosciences com Press OK to toggle between On and Off Installation Communi ne cations Description Range Serial Turn serial communication mode On to begin bi On Off directional communication To exit the communications mode reset the VIAFLO pipette or follow screen prompt Serial communication further allows in addition to the functions of Blue tooth Firmware updates Bi directional communication from PC to pipet
39. internals of VIAFLO Electronic Pipettes please contact your service technician Follow the instructions below for disassembly necessary for trouble shooting and autoclaving 6 1 1 Assembly of single channel pipettes Disassembly Disassemble the lower portion of the single channel Vision pipette as follows 1 Ensure the pipette is in the home or down position at the end of a pipetting cycle 2 Turn off power and unplug the power supply 3 Unscrew clockwise to remove the Cylinder Assembly a from the pipette Slide the Cylinder Assembly down from the pipette body This will expose the Piston Assembly b www integra biosciences com 43 VIAFLO Pipettes Operating instructions V04 Maintenance 4 Slide the following components from the Piston O Ring black and Seal white Assembly e Seal Retainer Spring d Seal Retainer c Set these components aside or place them in an autoclave pouch 5 Separate the Piston Assembly b from the upper portion of the pipette The Piston is held in place by a small magnet 6 With the Cylinder Assembly in hand turn counterclockwise to remove the black Sealing Ring f that holds the Ejector Sleeve h in place 7 Remove the Ejector Sleeve from the Cylinder Assembly 8 Remove the Ejector Sleeve Spring inside g 9 Set all components aside or place them in an autoclave pouch Reassembly Before reassembling the pipette check each component
40. ion is available for each mode Operation With the tip s in liquid press and release the Run key to initiate the aspirate step Press and release the Run key for every dispense Alternatively press and hold Run to execute paced dispenses The dispense number is shown on the display The pipettor will stop paced dispenses when it reaches the Last Dispense This aliquot contains the accumulated error from all prior dispenses You can choose to use this Last Dispense or discard it During the Last dispense press and hold the Run key to perform a two step blowout www integra biosciences com 33 VIAFLO Pipettes Operating instructions V04 Pipetting modes 5 2 3 Sample dilute mode Application Accomplish accurate sample dilutions by using diluent to chase small sample volumes from the pipet tips An air gap keeps liquid separated in the tips and helps to minimize carryover of diluent when aspirating the sample Options Steps Description Edit Aspirate 1 Sets the volume of the diluent aspirated first in the tip Air Gap Sets the volume of the air gap to keep both liquids separated Aspirate 2 Sets the volume of the sample in the tip Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation Press an
41. ions V04 Pipetting modes 5 2 7 Variable dispense mode Application Use this mode when differing dispense volumes are required This mode could be used to quickly set up a dilution series in plates or for feeding similar samples to different assay plates where different sample volumes are needed Options Steps Description Edit Count Sets the total number of dispensing steps Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Dispense Sets up to 5 different volumes for repeated dispensing The 1 5 total volume is automatically calculated Speed Sets speed of the current pipetting step Pace Sets the time interval between dispenses in repeat pipetting 1 long 9 short Help Help information is available for each mode Operation Press and release the Run key to initiate the aspiration of total volume Press and release the Run key to initiate each subsequent dispense The pipettor stops and beeps when ready for the Last Dispense step i e to purge the calculated waste volume amount Alternatively press and hold the Run key to execute paced dispenses The pipettor stops paced dispensing when it reaches the Last Dispense This aliquot contains the accumulated error from all prior dispenses You can choose to use this Last Dispense or discard it During the Last dispense press and hold the Run key to perform
42. isposable reagent reservoirs sterile individually sealed 4311 30 reservoirs per case Disposable reagent reservoirs sterile four sleeves of 4312 50 reservoirs per case Reservoir Base 10 pack 4304 Disposable reagent reservoirs sterile individually sealed 4321 30 reservoirs per case Disposable reagent reservoirs sterile four sleeves of 4322 50 reservoirs per case Reservoir Base 10 pack 4305 GripTips for VIAFLO Electronic Pipettes Part No Bulk 1000 tips per bag non sterile 4411 5 inserts of 384 tips non sterile GREEN CHOICE 4412 5 racks of 384 tips non sterile 4413 5 racks of 384 tips sterile 4414 5 racks of 384 tips sterile with filter 4415 5 inserts of 384 tips pre sterilized GREEN CHOICE 4416 Bulk 1000 tips per bag non sterile 4421 5 inserts of 384 tips non sterile GREEN CHOICE 4422 5 racks of 384 tips non sterile 4423 5 racks of 384 tips sterile 4424 5 racks of 384 tips sterile with filter 4425 5 inserts of 384 tips pre sterilized GREEN CHOICE 4426 Bulk 1000 tips per bag non sterile 4431 5 inserts of 96 tips non sterile GREEN CHOICE 4432 5 racks of 96 tips non sterile 4433 5 racks of 96 tips sterile 4434 5 racks of 96 tips sterile with filter 4435 5 inserts of 96 tips pre sterilized GREEN CHOICE 4436 63 www integra biosciences com VIAFLO Pipettes Operating instructions V04 1250 yl 5 ml 64 Accessories Bulk 1000 tips per bag non sterile 4441 5 inserts of 96 tips non ste
43. l and laboratory use General requirements EN 61326 1 Electrical equipment for measurement control and laboratory use EMC requirements Standards for Canada and USA CAN CSA C22 2 Safety requirements for electrical equipment for measurement No 61010 1 control and laboratory use General requirements UL Std No 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements FCC Part 15 Class A Emission Hudson New Hampshire USA April 29 2012 Sige HRO Gary Nelson George Kalmakis President VP Operations VIAFLO Electronic Pipettes Operating instructions V04 Table of Contents Chapter 1 Chapter 2 Chapter 3 Introduction TEM SSYMBOIS USCC is 5 iiber Re Seo E ats 7 1 2 Interided use aute inet e eter 7 1 8 Warranty Agreement enne 7 1 4 Safety n0168 21 an alten afe te eee Ed ns 8 Description of the device 2 1 SCOpe Of delivery i See RE EAT 9 2 2 Pipette configurations eeeseeeeeeeeeceneeeneeeenneeen nnne 9 2 3 Overview of the VIAFLO Electronic Pipettes 10 2 3 1 VIAFLO pipette parts sseemme 10 2 3 2 Back VIGw s id ede ER Here 11 23 3 Display i iia e bee eene 11 2 3 4 Touch wheelz rete te etes 12 2 3 5 Left and right arrow buttons seeeees 12 2 3 6 P rge bultori oec dde eris 12 2
44. lick stop applying pressure Discard your used tips If liquid is in the tips empty them by pressing the Purge button 6 Tips are easily removed by pressing the Tip ejector 8 43 Start pipetting 4 3 1 Pipetting Use the Touch wheel 3 to scroll to your desired pipetting mode and press OK 4 Selected parameters for the action you are about to perform will be displayed on the Run Screen ECIED E Insert the tips into the liquid to be transferred Press Repeat Dispense Aspirate 2504 and release the Run key 7 to aspirate the volume Sample Dilute Dispense 250yL selected in the first step of your protocol shown on EUN Pipet the Run screen EE tee E erui To execute subsequent steps press the Run Key For a detailed description see 5 2 Detailed description of pipetting modes on page 32 You can change the parameters of your pipetting mode at any time see the following sections 4 3 2 Blowout modes During the last dispense of a program a blowout is performed Liquid may be aspirated back into the tips when the pistons move back to the home position a process called blowin There are two ways to start the blowin Automatic blowout Pressing and releasing the Run key starts the dispense with blowout and automatic blowin You can choose a timed delay between the blowout and the blowin see Pipetting Delayed blowin under 3 3 1 Preferences on page 16 Two step bl
45. n amp Service and then the Calibration option Press OK Calibration Calibration Highlight the Pipet Current Factor Current CalbrationRemnder Iype Fede Press lt to edit the volume Service History Pipet 1 0000 Repeat 1 0000 HELP Edit Save www integra biosciences com 55 VIAFLO Pipettes Operating instructions V04 Maintenance Highlight Target Volume Vj on the Pipet Calib menu Press OK Actual Volume 1000 pU Factory Reset Calb Volume 1000yL Use the touch wheel to enter the Target Volume Always use the nominal volume 100 to adjust a VIAFLO pipette 125 ul in this example Press OK to save your selection Move the cursor to the Actual Volume V Factory Factor 1 0000 Current Factor 1 0000 Calibrate gt 4 COARSE FINED Use the touch wheel to enter the Actual Volume The actual volume is the mean weight corrected by the Z factor resulting in V the mean volume in micro liters see section 6 4 6 127 ul in this example Press OK to save your selection Pipet Caib Calibration Press gt to Calibrate and apply the factor universally Ce ee 3 Curent to all future Pipet mode motor movements This will Act 27 Type _ Factor zi or um Pipet y correct for any measured inaccuracies observed in MUR Repeat 1 0000 the validation process The Old and New Factors are t Sanii displayed You are returned to the Calibration menu urren
46. n dispensing e g for solutions with high viscosity or tendency to foam The dispense method used in this mode prevents introduction of any air into the sample Options Steps Description Edit Aspirate Sets the aspiration and dispense volume Last Dispense Sets the volume to leave in the tip until final blowout Asp Speed Sets speed uniquely for aspirating 1 low 10 fast Disp Speed Sets speed uniquely for dispensing 1 low 10 fast Speed Sets speed of the current pipetting step Help Help information is available for each mode Operation Press and release the Run key to initiate the aspiration The total volume aspirated is the sum of desired dispense volume and last dispense volume Depending on whether you press and release or hold the Run key at the start of a dis pense step you can repeat the aspirate and dispense process Press and hold the Run key Dispense the programmed volume without a blowout keeping the Last Dispense volume in the tip Upon release of the Run key you can aspirate the programmed volume again and repeat the dispense process as many times as needed Press and release the Run key Finish the aspirate and dispense process The programmed volume will be dispensed Last Dispense is the next active step To purge the Last Dispense volume with a two step blowout press and hold the Run key 36 INTEGRA Biosciences AG VIAFLO Pipettes Operating instruct
47. ng stand or the Universal Voltage Power Supply UPS that allows for pipetting while charging the battery see 8 1 Accessories on page 62 3 2 14 Charging the battery on a stand Use one of the different charging stands single position with two 4210 or four prong connector 4211 or four position with two prong connector 4215 to charge the battery Place the pipette on the charging stand by fitting the power receptacle 15 on the top back of the pipette over the prong connector on the top of the stand 2 socket at the base of the stand see 8 1 Accessories Plug the cable of the adequate power supply to the 1 on page 62 CAUTION e Always use the correct power supply for the charging stand 44 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Installation The pipette will turn fully on when placed on the stand When the dimming time is achieved the startup screen is displayed on the pipette When the turnoff time is reached the pipette turns off For disconnecting simply lift the pipette up from the stand 3 2 2 Charging the battery with UPS With the optional Universal Voltage Power Supply UPS 4200 you can use the pipette while charging through the line cord q Insert the UPS connector into the receptacle on the top back of the f pipette 14 Plug the UPS into a wall outlet It will take approximately 30 seconds of charging to begin running the motor The pipette will
48. nt V p zs 100 x V x V so V Calculation of random error Precision 96 To calculate the random error as the repeatability standard deviation s use the following equation The random error can also be expressed as a percentage by the coefficient of variation CV using equation 5 CV 100x V If the pipette is properly calibrated the Target volume V should equal the Actual volume V within the accuracy specifications of the pipette 6 4 8 Adjusting VIAFLO Electronic Pipettes Compare the calculated accuracy and precision values with the corresponding pipette specifications given in section 7 4 In case a VIAFLO Electronic pipette has not met the calibration specifications it needs to be adjusted This can easily be corrected by the pipettor s software There are two modes for calibration of VIAFLO Pipettes Pipette Calibration Mode for neat transfers and Repeat Calibration Mode for pipetting aliquots The industry standard is to test and present specifications using neat transfers This is aspirating and dispensing the same volume The pipette factor of the pipette s software determines the performance of neat transfers and is therefore adjusted in case a pipette performs out of specifications Adjusting a pipette in Repeat Calibration Mode is normally not required and is not described in this document The following example is for a 125 ul pipette Select Toolbox on the Main Menu Select the Calibratio
49. o Optimize your pipette s accuracy at a specific volume using aqueous liquid Enable accurate displacement of non aqueous based liquids with different specific gravities than water Enhance accuracy when pipetting heated or cooled liquids 6 4 4 Materials Precision balance with 0 01 mg readability Ohaus AP250D ASTM Class 2 or OIML E2 test weights Evaporation trap for balance Weighing vessel optimally the height to diameter ratio is at least 3 1 Measurement equipment for temperature humidity and atmospheric pressure Testo 435 2 Professional Distilled water Grade 3 according to ISO 3696 New non filtered GripTips matching the pipette volume see section 6 3 Single channel pipettor charging stand 2 beakers with distilled water 1 for pre wetting 1 for calibration procedure 6 4 2 Definitions Blow out Discharges any residual liquid from the tip Blow in After the blow out the piston moves back into home position This causes a slight intake of air or liquid if tips remain in the liquid Two step blow out To avoid an early blow in keep the run button pressed while dispensing remove the tips from liquid and then release the run button Pre wet The action of pre coating the inside of the liquid contacting parts with a thin film of the same liquid Additionally it equilibrates humidity of the air space inside tip and the pipette Touch off Touching the pipette tip against the surface liquid o
50. o Save this selection If 2 positions are chosen the Middle position is grayed out Tip Spacing Tip Spacing To change the tip spacing use the touch wheel to Select Step Set Spacing scroll to the position to be changed Press OK and Positions 3 9 0mm 115 140mm z First 9 0 mm the Tip Spacing menu appears Middl 11 5 Spacing mm p Midde 14 0 Press lt Close to reduce or gt Open to increase Spacing 9 0 mm the tip spacing incrementally The tips will physically lt lHome Tips Save Close Open gt move allowing you to visually match the tip spacing to the target vessels When the desired tip spacing is achieved press OK Make the desired changes to all positions Upon completion press gt to Save all the selections 26 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Operation 4 5 2 Tip spacing operation Select the pipetting function of interest Pipet Repeat Dispense Sample Dilute etc Then the bottom of the screen displays the Tip Spacing that the instrument is set at Press RUN to go Aspirate 250 0 pl Dispense 250 0 pl 90 115 1400 The First Middle and Last positions are displayed from left to right In the adjacent figure 9 0 mm is the First position 11 5 mm is the Middle position and 14 0 mm is the Last position The current position is highlighted in yellow Press lt or to move the tip position to the next value Pressing or
51. o attach the Cylinder Assembly to the body of the pipette Check the pipette performance after reassembly 6 1 2 Assembly of multichannel fixed spacing pipettes Before reassembling the pipette check each component for lint or dust particles It is recommended to replace the O Rings see 6 3 2 Changing O rings of tip fittings on page 48 s N a www integra biosciences com 45 Step 1 Disassembly Rotate counter clockwise to remove the lower housing Reassembly Rotate clockwise until a stop is felt then back off to align Step 2 Disassembly Gently pull to separate the upper and lower housing to expose the ball and socket Reassembly Gently push together the upper and lower assembly Step 3 Disassembly Disengage the ball and socket to separate the parts Reassembly Reengage the ball and socket to reconnect Step 4 Disassembly Remove the aluminum ring from the upper assembly Reassembly Slide the aluminum ring back onto the upper assembly The flanged surface goes into the upper assembly VIAFLO Pipettes Operating instructions V04 Maintenance Step 5 Disassembly Remove the chrome ring e Reassembly Slide the chrome ring back into place with the stepped surface up fa Step 6 Disassembly 4 Carefully remove the volume label to expose the screw Save the i label for reassembly Reassembly Reattach the volume label where previously removed Step 7 Di
52. om program Select Custom to create a personalized protocol Programs can contain up to 98 individual steps based upon five basic operations Aspirate Mix Dispense Purge Prompt and Tip spacing A prompt is any message that is displayed during the program it Custom Press gt to create a new program The program is n assigned a name automatically e g Custom 1 The custom programs can later be renamed with alphanumeric characters A Custom program must begin with an Aspirate Mix NewL Select Operation Prompt or Tip spacing No Programs Use the Touch wheel to select the first step and press OK e g Aspirate Edit Custom T Edit Custom T ul Speed n Set the volume value and press OK Aspirate 50uL 1004 Speed Y COARSE FNED 8 When starting with an Aspirate step followed by a Mix step the tips contain the aspirate volume after completing the last mix cycle When starting with a Mix step the tips are emptied upon completion of the last mixing cycle A Purge step is automatically integrated at the end of a program if the last programming step leaves liquid in the tips Set the speed for that step and press OK Next you have the options of adding a new step editing a step or deleting a step Select New step and press OK Use the Touch wheel to select the position where the new step should be inserted press OK and select an operation To define
53. on and dispense speed depending on liquid High viscosity liquids may require calibration High vapor pressure liquids may require pre wetting No dispensing aspirating Piston stuck or not con nected Motor not running O ring not installed Contact service Droplets on the tips 28 Temperature of liquid differs from that of air inside the tips Pre wet tips up to 3 times Liquid of low viscosity and high vapor pressure Increase dispensing speed INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 4 6 2 Electronic Problem Probable cause When pressing Run Low battery a Battery Low message appears on the Run screen Operation Remedy Re charge the battery in order to resume pipetting operation Plug the power cord into the pipette Note It takes 15 30 sec onds to store enough voltage to operate the pipette Display turns off completely Dead battery Charge the battery with a power cord or charge stand Sometimes a dead battery can be saved by unplugging the bat tery for 5 seconds Then plug in battery and try to turn on pipette If it powers up immediately con nect to power supply If pipette does not turn on after this proce dure replace with new battery Spinner response is A finger was on the spinner erratic and uncon when the pipette was turned Reset the pipette without touch ing the spinner
54. or 16 Pipetting speed 10 steps Pipetting technology Air displacement User interface Touch wheel color display 7 3 Pipetting speed Pipette Size Pipetting speed micro liters per second Speed 12 5 ul 125 pl 300 ul 1250 ul 5000 pl 1 0 44 4 48 11 26 45 16 174 40 2 0 88 8 86 22 29 89 36 345 07 3 2 12 21 38 53 79 215 69 832 93 4 2 56 25 83 65 00 260 63 1006 46 5 3 00 30 24 76 10 305 12 1178 29 6 3 97 40 00 100 65 403 55 1558 39 7 5 35 53 91 135 65 543 91 2100 43 8 6 83 68 89 173 33 695 00 2683 89 9 7 69 77 50 195 00 781 88 3019 38 10 8 79 88 57 222 86 893 57 3450 71 www integra biosciences com 57 VIAFLO Pipettes Operating instructions V04 Technical Data 7 4 Pipette Specifications VIAFLO Electronic Pipettes ISO8655 limits Volume Chan Part fane Incre maus Accuracy Precision Accuracy Precision nel No ments 496 S96 496 S96 ul ul ul 1 4011 0 5 12 5 0 01 1 25 5 00 4 00 16 00 8 00 6 25 1 50 0 80 3 20 1 60 12 5 1 00 0 40 1 60 0 80 1 4012 5 0 0 1 12 5 3 00 1 00 12 80 4 80 125 0 62 5 1 20 0 40 2 56 0 96 125 0 60 0 20 1 28 0 48 1 4013 10 0 0 5 30 2 00 0 60 13 33 5 00 300 0 150 1 00 0 30 2 67 1 00 300 0 60 0 15 1 33 0 50 1 4014 50 0 1 125 3 00 0 60 12 80 4 80 1250 0 625 1 00 0 30 2 56 0 96 1250 0 60 0 17 1 28 0 48 1 4015 100 0 5 500 3 00 0 75 8 00 3 00 5000 0 2500 1 20 0 30 1 60 0 60 5000 0
55. owout Perform a two step blowout to manually delay the blowin Press and hold the Run key to start dispense with blowout Remove the tips from the target vessel Release the Run key to start blowin www integra biosciences com 21 VIAFLO Pipettes Operating instructions V04 Operation 4 3 3 Recommendations for pipetting INTEGRA Biosciences recommends the following techniques for enhancing pipetting results These techniques are consistent with ISO standard 8655 2 Before liquid aspiration make sure the pipette tip is immersed approximately 2 3 mm into liquid in order to prevent any aspiration of air Pre wet GripTips After loading tips onto your pipette simply aspirate and dispense the desired liquid 2 3 times to coat the inside of the intended pipet tips Pre wetting ensures that the liquid and air inside the GripTips are at equal temperature further enhancing accuracy During incremental dispensing touch the GripTip s against the side of the well plates to prevent any liquid from clinging to the pipette tip This process is referred to as touching off For optimal precision when incrementally dispensing a series of volumes consider the following techniques Aspirate approximately 10 more liquid than is required and discard this extra volume to waste or back to the source Pre dispense the first volume As the piston motor changes direction from Aspirate to Dispense volumetric error can be observe
56. pette tips 1 3 Warranty Agreement The INTEGRA Biosciences Corp warranty applies to the original retail purchaser INTEGRA Biosciences will repair or replace at INTEGRA Biosciences s discretion any INTEGRA liquid handling device which is found to be defective in materials or programming from one year from purchase date If an instrument fails to function properly at anytime during the first year INTEGRA Biosciences should be contacted immediately INTEGRA Biosciences s warranty does not cover any damage or malfunction resulting from normal wear misuse or use in any way contrary to the instructions in the operating instructions Any evidence of misuse or abuse will void this warranty www integra biosciences com 7 VIAFLO Pipettes Operating instructions V04 Introduction 1 4 Safety notes VIAFLO Electronic Pipettes comply to the recognized safety regulations and is safe to operate VIAFLO Electronic Pipettes can only be operated when in perfect condition and while observing these operating instructions The device may be associated with residual risks if it is used or operated improperly by untrained personnel Any person operating the VIAFLO Electronic Pipettes must have read and understood these operating instructions and particularly the safety notes or must have been instructed by supervisors so that safe operation of the device is guaranteed Use only an INTEGRA battery Regardless of the listed safety notes additional ap
57. plicable regulations and guidelines of trade associations health authorities trade supervisory offices etc must be observed Do not open or modify the VIAFLO Electronic Pipettes in any way Repairs may only be performed by INTEGRA Biosciences AG or by an authorised after sales service member Parts may be replaced with original INTEGRA Biosciences parts only WARNING Do not use the VIAFLO Electronic Pipettes near flammable material or in explosive areas Also do not pipette highly flammable liquids such as acetone or ether When handling dangerous substances comply with the material safety data sheet MSDS and with all safety guidelines such as the use of protective clothing and safety goggles CAUTION Do not immerse the VIAFLO Electronic Pipettes in liquid The fluid can damage internal parts Avoid pipetting of liquids whose vapors could attack the materials PA polyamide POM polyoxymethylene FPM fluor rubber NBR nitrile rubber CR chloroprene silicone Corrosive vapors could also damage metallic parts inside the device Sor NOTE Prolonged exposure of the VIAFLO Electronic Pipettes to UV light can cause discolouration and or yellowing of the control unit However this will not affect the performance of the device in any way 8 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Description of the device 2 Description of the device 2 1 Scope of delivery VIAFLO pipette Recharg
58. r side of well plate to release any sample liquids that might be on the end of the pipettor tip 50 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance 6 4 3 Test Conditions and Environment Tests and calibrations should be performed in conditions and environment according to ISO 8655 6 standard Temperature needs to be between 18 25 C and remain constant 0 5 C through out the calibration Optimal relative humidity of the environment is gt 50 and around the dispensing posi tion 80 GripTips pipettes and distilled water need to be in the calibration laboratory for at least 2 hours prior to calibration to reach temperature equilibrium with the environ ment The balance must be validated using reference weights before and after a measuring series A low and high volume weight should be used E g 100 g Mettler Toledo 11119250 10 g Mettler Toledo 11119220 1 g Mettler Toledo 11119190 10 mg Mettler Toledo 11119130 CAUTION STOP Always use new pre wetted GripTips for leak test and calibration 6 4 4 Pre wetting of tips Pre wet new GripTips three times prior to starting tests and calibrations This is required every time a tip is changed 1 Attach a new unused GripTip which correspond to the volume range of the pipette directly from the rack without touching the tip by hand 2 Use a separate waste container for pre wet dispenses 3 In the Pipet mode program t
59. rate liquid 1 160 ul e g diluent With tips in liquid 1 press Run key 2 Aspirate air 20 ul Move tips out of liquid and press Run key 3 Aspirate liquid 2 50 ul e g reagent With the tips in liquid 2 press Run key 4 Dispense 230 ul Press and hold Run key until liquid is dispensed and tips are removed from the liquid two step blowout 5 Mix 3x 200 ul Press Run key Purge does not need to be programmed The residual liquid is dispensed into the waste container Press and hold Run key until liquid is purged and tips are removed from the liquid two step blowout For a detailed description see 4 4 1 Edit option on page 23 42 0700 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance 6 Maintenance WARNING Always turn off power and disconnect the VIAFLO Electronic Pipettes from the mains when carrying out maintenance work 6 1 Cleaning The materials used on the exterior of the VIAFLO Electronic Pipettes support regular cleaning intervals Clean the external components with a lint free cloth lightly soaked with mild soap solution in distilled water or with a 70 dilution of Isopropyl or Ethanol Never use acetone or other solvents WARNING Do not immerse the entire pipette into a cleaning solution or spray cleaning solution directly onto the exterior body of the pipette as this can potentially damage internal electronics If liquid ever enters the
60. rile GREEN CHOICE 4442 5 racks of 96 Tips non sterile 4443 5 racks of 96 Tips sterile 4444 5 racks of 96 Tips sterile with filter 4445 5 inserts of 96 tips pre sterilized GREEN CHOICE 4446 Bulk 250 tips per bag non sterile 4451 Individually wrapped 100 tips per case sterile 4456 INTEGRA Biosciences AG
61. ry out steps 1 5 for closed and open tip positions min and max tip spacing Signs indicating a leak 1 During the mix cycle in the leak test the liquid level of 1 channel is decreasing NOTE A decreasing liquid level at aspiration could be an indication of a slow leak Performing a retest at 10 mixes may help identify a slow leak 2 Liquid is left in a tip after the last dispense during the leak test 3 One or more channels show air bubbles during the mix cycle in the leak test 4 Droplets are forming when holding the pipette in the air for 20 seconds even if a pre wet was performed 5 The liquid levels are not equal on all channels after aspiration If the pipette is leaking change O rings see 6 3 2 and lubricate pistons see 6 3 3 or contact your service technician 52 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance 6 4 6 Obtaining the Actual Volume INTEGRA certifies the pipettes at 10 50 and 100 of the nominal value For each volume 5 measurements are taken from all channels General a Always use new unused GripTips also when changing the test volume e g from 125 ul to 12 5 ul b Always pre wet section 6 4 4 when using a new GripTip For a low volume mea surement first select the maximum volume for the pre wet and then change to the volume to be measured c After dispensing perform a touch off to make sure no liquid remains on the tip Gravimetric testing
62. sassembly Remove the two small screws Reassembly Replace the two small screws on the front housing where the volume label had been removed if not completed in the previous reassembly step Step 8 4 Disassembly Remove the two screws from the rear housing Reassembly Replace the small screws from the rear housing Step 9 Disassembly Separate the housing from the cylinder piston assembly Reassembly Place the rear housing b back onto the front housing piston assembly a 460 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Maintenance Step 10 Disassembly Remove the foam tape from the front and rear carefully note the position of the tape and save for reassembly in exactly the same position c Reassembly Reattach the foam tape to the front and rear housing where it was removed previously Place the piston assembly in the front housing registering on the two bosses Turn over and replace the two small screws do not over tighten Step 11 Disassembly Unscrew the manifold screw one full turn 2 to 6 screws depending on volume size and pull the piston plate d to the up position Reassembly Be sure the piston plate is in the up position and tighten the manifold screws exactly one full turn you should feel light pressure on the Screws 6 2 Sterilization If the surface of the VIAFLO Electronic Pipettes have been in contact with biohazardous material the
63. sciences AG representative To find out name and address of your local representative go to www integra biosciences com Further information and operating instructions in other languages are available from info integra biosciences com 6 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Introduction 1 Introduction These operating instructions contain all the information required for installation operation and maintenance of the VIAFLO Electronic Pipettes This chapter informs about the symbols used in these operating instructions the intended use of the VIAFLO Electronic Pipettes and the general safety instructions 1 4 Symbols used The operating instructions specifically advise of residual risks with the following symbols WARNING This safety symbol warns against hazards that could result in injury It also indicates hazards for machinery materials and the environment It is essential that you follow the corresponding precautions CAUTION This symbol cautions against potential material damage or the loss of data in a microprocessor controller Follow the instructions NOTE This symbol identifies important notes regarding the correct operation of the device and labour saving features 1 2 Intended use VIAFLO Electronic Pipettes are microprocessor controlled and stepper motor driven pipettes They are used for aspirating and dispensing liquids in the volume range of 0 5 5000 ul using GripTip pi
64. t Factor 1 0000 f Calbrate gt Edit Save Press to Save your settings A message will indicate that the pipette recalibration is complete To check and revalidate repeat Steps 1 7 in Section 6 4 6 In case a calibration reminder was set it can be reset in the Calibration Reminder menu for either time and or cycles NOTE In case an error message appears when setting the new correction factor step 8 follow these steps a Select the Repeat factor in the calibration menu and enter the target and actual measured volume b Press calibrate c Then select Pipet factor and enter target and actual volume d Press calibrate e Both factors should be the same now f Press Save 6 5 Equipment disposal The VIAFLO Electronic Pipettes must not be disposed of with unsorted municipal waste Dispose of the VIAFLO Electronic Pipettes in accordance with the laws and ED regulations in your area governing disposal of devices 56 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Technical Data 7 Technical Data 7 1 Environmental conditions Operation Temperature range 5 40 C Humidity range lt 85 RH non condensing Altitude range lt 2000 m 7 2 Specification of the device Power Requirements 100 240 VAC 50 60 Hz Universal Power supply Input 100 240V 50 60Hz Output 6V 0 1 75A DC set at 0 5 A Pipetting channels single 4 6 8 12
65. tandard programs Custom programs Calibration Toolbox VOYAGER Password protect Protect the access to the write pro tect menu by selecting On Edit password if password protect is On To enter a password use the Touch wheel to highlight a charac ter and press OK Press gt to save the password The password must be entered before you can access the write protect menu Keep the password in a safe place 3 3 7 Password key This menu displays the encrypted password if set Should you lose your password contact INTEGRA Biosciences Corp to retrieve your password using these numbers www integra biosciences com 19 VIAFLO Pipettes Operating instructions V04 Operation 4 Operation 4 1 Turn on off the device Turn on Press and release the Run key 7 to turn on the pipette CAUTION Remove hands from the Tri lobe tip interface at switch on and during homing Also do not touch the touch wheel because it is calibrated during the start up process The pipette flashes the startup screen and performs a full motor homing routine ensuring the motor is in the run position Home is the base point for the pipette Homing is the process whereby the pipette motor moves the piston s to a sensor position This position ensures that no liquid remains in the tips For the Voyager homing also includes the tip spacing motor After homing the tips move to the last position they occupied A
66. te Bluetooth Each pipette needs its own Blue Tooth chip Blue Tooth On Off communication has an open field connectivity of about 300 meters It can be used for File handling of pipetting protocols Image downloads Service history tracking 3 3 4 Owner Information Owner E information Description Range VIAFLO The first line is highlighted Press gt to enter personal information for your VIAFLO Electronic Pipettes Use the Touch wheel to highlight a character and press OK You can press lt to Delete the last charac ter entered After entering the desired text press gt to Save In addition information about your VIAFLO Electronic Pipettes such as serial number SN pipette size indication of single or multi channel and version number are displayed automatically 18 INTEGRA Biosciences AG VIAFLO Pipettes Operating instructions V04 Installation 3 3 5 Help Language Help m Language Description Range Help You can choose the language in which all help screens Language are displayed English French German Spanish simplified Chinese Japanese Scroll to the desired language and press to Save 3 3 6 Write protect Select this option to protect programs and menu options from inadvertent modification The pipetting programs can still be used Write protect Description Range Select an option and press OK to switch protection On or Off On Off S
67. ted pace Release the Run key to stop the paced dispense Press Run to continue dispensing Run Repeat Disp HEB un Repeat Disp mz Use the Touch wheel to select the desired Pace Press RUN to go Press RUN to go option and press OK Asprate 12504L Aspirate 1250 pL Dimes 1004 Dispense 100g Select the pace from None 1 slowest to 9 fastest 5d Press OK to save your setting I Speed 8 4 4 5 Count Mix Cycle and Rows The Count Mix Cycle and Rows steps are used in various modes Each is accessed with the Edit option Use the Touch wheel to highlight the step and press OK Count sets the number of dispensing steps Mix Cycle sets the number of mixes In serial dilution mode Columns sets the number of columns A column indicator will notify the number of dilutions performed Columns first number and Mix Cycles second number are tracked on the display Mix Cycles are shown in red when mixing A green dot on the column number indicates the active program step Select a desired value Press OK and then press gt to save your setting s 4 4 6 Help The Help information available for each mode describes the mode operation You can select Help in two ways Main Menu Run Pipet While in the Main menu highlight a pipet mode Press RUN to go then press lt to select the Help option Repeat Dispense Aspirate 250 uL UP fi he Hel A f Sample Dilute pea WE While in a pipet mode find the H
68. ttes 55 6 5 Equipment disposal sese 56 Technical Data 7 1 Environmental conditions cccccccececeecceeseessseseeceeeeeeeeeeeanenens 57 7 2 Specification of the device 57 7 9 Pipetting speed Re pe Un 57 7 4 Pipette Specifications 58 1 5 Z Correction FactolS rin etu e et ie e eese teo 61 Accessories 8 4 ACCGSSOLIOS a oic e re er ee Pet dog via e e TE DdRd 62 8 2 Con uM A Ee a ra aaa a aa a aara aeae atoia n eren 62 www integra biosciences com 5 VIAFLO Pipettes Operating instructions V04 Imprint Copyright 2012 by INTEGRA Biosciences AG All rights reserved No part of this work may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopying and recording or by any information storage or retrieval system except as may be expressly permitted in writing by INTEGRA Biosciences AG This operating instruction manual applies to firmware version 2 44 or higher until a newer revision is released VIAFLO Vision Voyager VIALINK and GripTip are trademarks of INTEGRA Hudson NH Manufacturer INTEGRA Biosciences AG INTEGRA Biosciences Corp CH 7205 Zizers Switzerland Hudson NH 03051 USA T 41 81 286 95 30 T 1 603 578 5800 F 41 81 286 95 33 F 1 603 577 5529 info integra biosciences com www integra biosciences com Customer service Please contact your local INTEGRA Bio
69. xecute They are described in the fol lowing sections Custom step based programming mode You can create and store up to twenty multi stepped pipetting protocols on the pipettor using the five basic functions of Aspi rate Mix Dispense Purge and Prompt presented in 5 2 Detailed description of pipetting modes on page 32 The custom programming mode is described in 5 3 Custom step based programming mode on page 41 5 1 Overview pipetting modes The table below provides an overview of the selectable pipetting modes All modes are accessed from the Main Menu Use the Touch wheel to scroll to your desired pipetting mode Pipetting mode Description Pipet Allows liquid transfers when aspirate and dispense volumes are equal Repeat Dispense Allows dispensing multiple aliquots of the same volume without refilling the tips after each dispense for fast microplate filling and processing Sample Dilute Allows aspirating of sample and diluent divided by a defined air gap into one tip followed by a complete dispense Pipet Mix Allows multiple mixing by aspiration and dispensing of defined volume for automatic re suspension of pellets Manual Pipet Allows the operator to control the aspiration and dispensing up to the set volume Reverse Pipet Allows liquid transfers of viscous or high vapor pressure liquids by preventing introduction of any air into the sample The aspiration volum
70. y must be decontaminated in accordance to good laboratory practice Whisk and wipe the surface with a lint free cloth lightly soaked e g with the following disinfectants Ethanol 70 Microcide SQ 1 64 Glutaraldehyde solution 496 Virkon solution 1 396 Follow the instructions provided with the reagents It is not recommended to autoclave the VIAFLO pipettes except for decontamination before sending them for service and repair Only the lower assembly of the VIAFLO single and multi channel pipettes can be autoclaved WARNING A service is required after autoclaving the VIAFLO pipettes Do not autoclave the entire unit The extreme heat can damage the display and other electrical components VOYAGER pipettes can NOT be autoclaved they might be damaged As found calibration measurement report indicating before data is not possible after autoclaving www integra biosciences com 47 VIAFLO Pipettes Operating instructions V04 Maintenance 6 2 1 Autoclaving the disassembled components Place the disassembled components see 6 1 to steam autoclave in an autoclave pouch Single channel Multichannel fixed spacing z gt tf 4 t You may autoclave the components at 121 C 1 bar overpressure for 20 minutes After autoclaving send the pipette for service 6 3 Servicing 6 3 4 Shipping to INTEGRA Biosciences For any service or repairs purchaser is responsible for initial shipping cost of product INTE

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