VIAFILL Operating instructions
Contents
1. www integra biosciences com 17 VIAFILL Operating instructions V02 Installation 3 3 4 Sounds Turn beep tones ON or OFF by selecting the buttons YES or NO Sounds can be managed for PROGRAM END and ERRORS Press SAVE to continue 3 3 5 Communication TOOLBOX SycoMMUNICATION Turn the communication capability ON or OFF This COMMUNICATION XS enables users to communicate to the VIAFILL using API commands via a PC Pa Pe 3 3 6 Stage alignment TOOLBOX menaa J Adjust the plate stage for differing well plate and tubing 96 well 8Ch 6 well set combinations This option ensures the dispensing 384 well 8Ch 12 well 384 well 16Ch 24 well M LIIL IL ILIZ 1536well 16Ch 48 well nozzles are centered over the microplate wells The Stage Alignment screen will appear once the button is pressed STAGE ALIGNMENT 96 wall First press the MOVE button to position the plate in the He teach position Press FAST or SLOW and CONTINUOUS or STEP to adjust the stage movement speed Use the ARROW buttons of Z AXIS to position the dispensing tips above the plate Adjust the X and Y stage position to the desired location by the ARROW buttons Press SAVE to update and save current settings 3 3 7 Owner Information Press the NAME button to change the owner and press SAVE The SERIAL NUMBER SOFTWARE version and FIRMWARE version is also displayed here Pres2. seeeen 46 6 4 Decontamination of the dispensing cassettes 47 6 5 Cleaning the tpsa innin aea a e a aaa e A 47 0 6 SOTVICINO iiie S N een e re ee ee 47 7 Calibrations een tide eee dane eee ends ee 48 6 8 Equipment disposal ce eeeseeeeeseeeeeeeeeeeneeeeesneesesaeeeeeeeeeeetereaes 48 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Chapter 7 Technical Data 7 1 Environmental conditions 49 7 2 Specification of the device 49 7 3 Dispense speed 7 4 Dead volumes for dispensing cassetes 50 7 5 Specification of the stacker optional ssssssss 50 7 6 Accuracy and Precision Specifications ssessssss 51 Chapter 8 Accessories and consumables Bi 4 ACCOSSOLIGS uobis iR ie ik tee OEE ot 52 8 2 Consumables 4 n ne ttt ee es 52 www integra biosciences com 5 VIAFILL Operating instructions V02 Imprint Copyright 2012 by INTEGRA Biosciences AG All rights reserved No part of this work may be reproduced or transmitted in any form or by any means electronic or mechanical including photocopying and recording or by any information storage or retrieval system except as may be expressly permitted in writing by INTEGRA Biosciences AG Manufacturer INTEGRA Biosciences AG INTEGRA Biosciences Corp CH 7205 Zizers Switzerland Hudson NH 03051 USA T 41 81 286 95 30 T 1 6
3. Washing Caumn Washing Column Washing The wash module will dispense wash fluid to one column and then aspirate the fluid from the same column before washing the next column Press SAVE to continue 2 Select the plate that is located on the deck Press the Plate option in the WASH menu PLATE SEECTON ea Match the plate on the screen to the plate that is seated EINEN cs 8 gum on the VIAFILL plate deck After selecting the correct sem s uw Eee plate press the SAVE button Te oe 3 Select the source container Click on a SOURCE option in the WASH menu SOURCE e 7 Only the Bottle can be utilized with the Wash tubing set j t p Press SAVE to continue S aa Lu Facas Peseroe Pole EN c 4 Select the tubing set Press the button below TUBE SET in the WASH menu TUBING SET WASH Ce Select the 8 or 16 Channel Washing tubing set The 8 channel option is used with a 96 well plate Change the plate selection to a 384 or a 1536 to allow the selection of tt a oc the 16 Channel Wash tubing set Press SAVE to continue www integra biosciences com 37 VIAFILL Operating instructions V02 Operation 5 Select which wells to fill Press the Plate Map option in the WASH menu ej Press the YES and NO buttons to select and deselect columns Press the ALL WELLS button to select or deselect the entire plate PLATE MAP 96 Well Plate ee t e T
4. 83 Tubing selection for 6 through 48 well dispensing 15 3 3 Toolbox adapt your VIAFILL s 16 3 931 Prime Dpurge xe ce iet Hb ee eite RES 16 3 3 2 Mix pump optional eeeee 17 3 9 3 Pipet direction eee RT 17 334 SONAS ecc ere d e Es Pate Pec pese 18 93 5 GOMMUNICATION 5 menie Sei nic ReDR 18 3 3 6 Stage alignment 18 3 3 7 Owner Information esee 18 3 9 8 RECOVER ciae Wie eh edu Mtr de Port Sota 18 3 3 9 Pump calibrations irian a 19 3 9 10 Washi Opona lsin isna aisne itcm tnt 19 3 3 11 Stacker optional sessseeeeeeee 20 3 4 Hlelpsscreers eon nde neq aede SNS 20 Chapter 4 Installation of optional accessories 4 1 Wash module 4 o eid npe aite pe aana ANELEE Eaa AnS 21 4 1 1 Materials included ceecccecseeeeeeceeeeeceseeeeeeeseeeeeeeeenesees 21 4 1 2 Description of the wash module eesssss 21 4 1 3 Wash module set up eese 22 www integra biosciences com 3 VIAFILL Operating instructions V02 Chapter 5 Chapter 6 4 2 Plate Stacker dese e gne dde e e ee a 23 4 2 1 Materials included ssee 23 4 2 2 Description of the stacker seseeeee 24 4 2 3 Stacker setup iio trt bveniedeiteieameten 24 4 3 External mixing pump sseseeenm emere 27 4 38 1 Materials included ccc
5. Kit 100 ul 52 096 1 596 400 plates 25 ul 4 0 2 0 16 channel 125 ul 2 0 1 5 GripTip Kit 40 ul 42 0 lt 1 5 200 plates 10 ul 5 0 3 096 www integra biosciences com 51 VIAFILL Operating instructions V02 8 Accessories and consumables 8 1 Accessories Accessories and consumables Accessories Part No External Mixing Pump to keep cells in suspension by gentle circulation 5905 Plate Stacker base unit 5910 Plate Stacker Chimney 25 plate capacity 1 each 5915 50 plate capacity 1 each 5916 Wash module for plate washing in combination with 5950 wash set 5960 or 5965 8 channel wash set for use with wash module 5960 16 channel wash set for use with wash module 5965 8 2 Consumables Dispensing cassettes Part No 8 channel small bore plastic dispensing cassette sterile 0 5 999 ul 5722 5 per case 8 channel standard bore plastic dispensing cassette sterile 5 999 9 ul 5724 5 per case 16 channel small bore plastic dispensing cassette sterile 0 5 999 ul 5742 52 5 per case INTEGRA Biosciences AG VIAFILL Operating instructions V02 Accessories and consumables GripTip kits Part No 8 channel GripTip kit enables use of 300 ul GripTips 5820 includes 5821 5822 5823 8 channel tubing set to connect 8 channel pipetting head 5821 8 channel pipetting head for the use of 300 ul GripTips 5822 GripTip adaptor for the installation of the pipetting he
6. V02 Installation 4 Stretch the right black cartridge 26 upwards and set into the groove on top of the pump head 23 5 Ensure all 16 or 32 adjustment screws d are on the inside of the cartridges and the tubings on the outside d O Q 8g 7 Pull the sliver lever back up into position as shown by the arrow Both black cartridges should now be in the upright position Lift the black pump housing back into position Place the dispensing cartridge 27 into standard cassette holder 3 2 2 on the pipetting arm Put the source tubing into the source container www integra biosciences com 13 VIAFILL Operating instructions V02 Installation Alternatively GripTip Kit 8 Open the lever e on the back of the left side of the GripTip adaptor and place the GripTip adaptor on the A pipetting arm Secure the GripTip adaptor with the two screws beginning with the frontmost 9 Place the dispensing cartridge into the GripTip adaptor 10 Load GripTip pipette tips onto the pipetting head and slide the head into the GripTip adaptor 11 Tighten the lever of the GripTip adaptor by moving it to the front right side as shown by the arrow Put the source tubing into the source container 14 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation 3 2 2 Changing dispensing cassette holder or GripTip adapter t 1 If the dispensing cassette ho
7. a manner that protects human health and the environment Thank you 48 SNTEGRA Biosciences AG VIAFILL Operating instructions V02 7 Technical Data Technical Data 7 1 Environmental conditions Operation Temperature range 5 35 C Humidity range lt 80 RH non condensing Altitude range lt 2000 m 7 2 Specification of the device Dimensions L x W x H 37 5 cm x 30 5 cm x 24 0 cm 14 76 x 12 00 x 9 45 VIAFILL weight 10 66 kg 23 5 lbs Power requirements 100 240 VAC 50 60 Hz Dispense speeds 18 seconds 100 ul to 96 well Compatible plate formats 6 12 24 48 96 384 1536 Shallow amp Deep Well Plates Volume range 0 5 ul to 9 999 pl Bore size of plastic tubing sets Small 0 3 mm 0 012 large 0 5 mm 0 018 User interface Touch Screen Amperage rating 0 8 Amp Max Voltage tolerance 10 Outlet type VIAFILL must use grounded outlets only Fuse rating AC250V T3 15AL www integra biosciences com 49 VIAFILL Operating instructions V02 Technical Data 7 3 Dispense speed Dispensing option Well plate 1 ul 10 pl 50ul 100 ul 8 Channel small bore cassette 96 well 5 66s 76s 15 18 2338s 8 Channel small bore cassette 384 well 11 95 179s 477s 8455s 8 Channel standard bore cassette 96 well NA 5 85 7 38 9 8s 8 Channel standard bore cassette 384 well NA 11 6s 3183s 2625s 16 Channel small bore cassette 384 well 76s
8. alkaline or saline solutions to prevent damage 6 3 Decontamination of the VIAFILL Decontamination is not required for the proper functioning of the VIAFILL Only if any surfaces have been in direct contact with biohazardous material they must be decontaminated in accordance to good laboratory practice e g with the following disinfectants Ethanol 70 Microcide SQ 1 64 Glutaraldehyde solution 496 Virkon solution 1 396 Follow the instructions provided with the reagents 4600 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Maintenance 6 4 Decontamination of the dispensing cassettes The pipetting heads are autoclavable along with the dispensing cassette holder Dispensing cassettes tubing and wash sets can be autoclaved a maximum of ten times each at 1 bar pressure at 121 C for 20 min Replace dispensing cassette after ten autoclave cycles CAUTION After autoclaving the dispensing cassettes must cool down to room temperature before use Do not autoclave any other parts of the VIAFILL than specified Alternatively the dispensing cassettes can be decontaminated in Virkon 1 396 Ethanol 70 96 or glutaraldehyde 4 solution for 10 minutes Afterwards rinse them with deionized water 6 5 Cleaning the tips NOTE Place the source tubing in a separate bottle filled with deionized water to prevent particles re entering the reagent If any tips of the standard dispensing cassettes are clogged try one o
9. aspiration tube aspirates the reagent from the well plate The two options are linear and circular A different wash pattern can be selected for both a 96 and a 384 well plate Selecting a plate will take you to the wash direction screen Wash Direction 96 well 7 First press the PO MOVE button to position the plate in iti aM the teach position Use the ARROW buttons of Z AXIS to position the nozzles above the plate Adapt the teach position so that the nozzles target the center of the wells press SAVE and select PO Press TP Move and select one of the 4 positions to be P4 defined where P1 and P2 are the left and rightmost P3 and P4 the lower and topmost positions within the wells P1 PO P2 The plate and the pipetting arm move to the selected position Adjust the X and Y position by the ARROW P3 buttons Press SAVE and select the previously adjusted position P1 P4 Press BACK to continue Press the Rinse button to set the Rinse frequency and duration The purpose of the rinse feature is to clean the wash tubing set in between plate washes The rinse and wash fluids are contained in different bottles www integra biosciences com 19 Installation VIAFILL Operating instructions V02 TOOLBOX WASH RINSE PUMP SLOW MEDIUM FAST INTERVAL TIME TO ON 5 RINSE AUTOMATICALLY Xs LZ minute 123 sec 2 e RINSE RINSE TIME 3 3 11 Stacker optio
10. button below TUBE SET in the SERIAL DILUTE menu Select the GripTip tubing set that is currently installed in the VIAFILL see Dispensing cassette on page 11 and press SAVE TUBING SET 97 GripTip kit m Lco Select the 300 ul 8 Channel Pipet Tip tubing set Currently the 300 ul 8 Channel Pipet Tip tubing set is the eh i a only available in the SERIAL DILUTE menu Longe 2 Choenel 16 Ohennel 4 Select which wells to fill Press the Plate Map option in the selected pipetting menu e Press the YES and NO buttons to select and deselect 4 columns Press the ALL WELLS button to select or deselect the entire plate Press the SAVE button to continue PLATE MAP 96 Well Plate mmm mm ye ME NEG NU 7 8 9 n uo 289090090099 Al Wells i SAVE 840002 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 Select dispense volume s Press the Volume button in the SERIAL DILUTE menu VOLUME SERIAL DILUTE 9 Select YES or NO to activate or deactivate the desired SAMPLE eee xi Options Press the value buttons to activate the keypad eus pid asnar slo e g volume ul cycles wait sec Enter the desired pow outs tio 75 5 25 19 parameters and press ENTER Purge Lost Yes swe Asbirore LES a JL Sample This is the sample volume that is aspirated dispensed into each well Mix Sample Mixes the sample in the
11. cable to the marked port on the back panel of the VIAFILL 9c Attach the power cords Turn on the power for both the VIAFILL and the Stacker at the same time REPEAT DISPENSE l E Jte j The STACKER button f will appear red if the stacker is egeo Dupense M Yu ums installed correctly and activated Bottle Partial Plate 123 CLR ube Sar sd Z Plastic LG 2224 i0 Ca Eea 26 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation of optional accessories 4 3 External mixing pump The external mixing pump allows users to keep cells in suspension while simultaneously dispensing to microwell plates With the VIAFILL cells are not damaged while kept in suspension at all times 4 3 4 Materials included Confirm the contents of the external mixing pump set These include Mixing pump Tubing set RS232 Connection cable 4 3 2 agus of the external mixing pump 51 Mixing pump with mix speed adjustment knob T a 52 Source container 53 Mix pump tubing 51 4 3 3 Mixing pump set up 1 Remove the plastic cover for the mixing pump Press the tubing set into the mixing pump track Replace the plastic housing 2 Plug the RS232 cable into the back of the mixing pump and into the back of the VIAFILL The external mixing pump does not require its own power cable 3 Turn on the VIAFILL and press the red TOOLBOX icon in the upper right corner Select the Mix pump option from the TOOLBOX main men
12. first chimney position This ensures that the microwell plates return to the same order that they were loaded in www integra biosciences com 43 VIAFILL Operating instructions V02 5 7 Troubleshooting FAQ 5 7 1 VIAFILL base unit Problem Probable cause Peristaltic pump Tubings are not correctly does not rotate positioned on the roller wheel Operation Remedy Turn off VIAFILL See section 3 2 1 to confirm the tubing set is installed correctly Plate mover does Software malfunction not move Switch the device off and on Contact service technician Position error of Tip heights defined too low pipetting arm Define appropriate clearance heights See section 5 4 for how to adjust tip heights The dispensing The stage alignment has not nozzles GripTips been set correctly do not line up with the center of the Select the Stage Alignment option in the toolbox See section 3 3 6 for how to adjust the stage alignment wells The desired height The height was not entered Once the desired height is was not saved correctly selected press ENTER and then press SAVE This will ensure the height is saved properly The 384 stage align Belt movement malfunction ment does not allow the dispensing nozzle to reach the center of both wells on a 384 well plate 44 Contact service technician INTEGRA Biosciences AG VIAFILL Operating instructions V02 5 7 2 Stacker Problem P
13. measurement control and laboratory use EMC requirements Standards for Canada and USA CAN CSA C22 2 Safety requirements for electrical equipment for measurement No 61010 1 control and laboratory use General requirements UL Std No 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements FCC Part 15 Class A Emission Hudson New Hampshire USA March 23 2012 Gary Nelson George Kalmakis President VP Operations VIAFILL Operating instructions V02 Table of contents Chapter 1 Introduction 1 1 1 2 1 3 SVMDOIS USEC et eto etie ditto tet tetto arte tb s 7 Intended Use cete ge tie ee oed iei dete 7 Safety NOS ede ck cef fede ettet ec eh dt 8 Chapter 2 Description of the device 2 1 2 2 Scope of delivery ti oae UE Eds 9 Overview of the VIAFILL eseseeeeeeeenennneen 9 2 24 FTODEVIOW siet taatateti eee eet 9 2 2 2 Back VIGW eee tdeo aer e ero pee e e ode 9 2 2 9 Display ien eg Ae 10 2 2 4 P iriplhead theta neces tes cr etd 11 2 2 5 Dispensing cassette sseee 11 Chapter 3 Installation 3 1 Operating environment sssssseeeeeeeneneene 12 3 2 Assembling the instrument see 12 3 2 1 Installing the dispensing cassettes 12 3 2 2 Changing dispensing cassette holder or GripTip adapter 15 3 2
14. position to overwrite TuS 4 The keypad will appear to enter a custom name L 1 7 5 Once the Custom name is entered press SAVE sve Te foley ATI TeETSSPTS THE DIDI 5 5 2 Copy a program From the the MAIN MENU select one of the CUSTOM programs Change all desired parameters and save the program with a new custom name 5 5 3 Custom programs In addition to saving a program as a custom program the VIAFILL software allows the user to start with a blank custom program This allows a user to select the function Repeat Dispense Variable Dispense Serial Dilution or WASH All other settings should be chosen the same as any other program mode Open a new custom program 100 Custom 1 BI A lusus 4X4 Laam Select any of the 95 CUSTOM buttons from the MAIN Sont 1 MENU This will take you to the Custom programming main screen JAspirate Bottle Partial Plote e loras aa i Siow RN od wep ZZ Select the desired function Unlike the other modes the Function key can be changed in custom programming Press the function key and select the desired function This will determine which options are available Tubing sets plates and source containers To complete the rest of the function programming see the individual programming instructions for each option 5 3 Finally name the custom program Press the Custom text box to display the keypad enter the desired Custom nam
15. power cable from AC Inlet Outlet Requirement Only use grounded outlets when plugging in the VIAFILL DO NOT USE UNGROUNDED OUTLETS Do not use the VIAFILL near flammable material or in explosive areas Also do not pipette highly flammable liquids such as acetone or ether When handling dangerous substances comply with the material safety data sheet MSDS and with all safety guidelines such as the use of protective clothing and safety goggles 10 Prolonged exposure of the VIAFILL to UV light can cause discolouration and or yellowing of the control unit However this will not affect the performance of the device in any way INTEGRA Biosciences AG VIAFILL Operating instructions V02 Description of the device 2 Description of the device 2 1 Scope of delivery VIAFILL base unit Reservoir with waste nozzle Waste tubing 25 ml reservoir sample pack 8channel plastic dispensing cassette sterile 5 9999 ul Power cord Operating instructions potential transportation damage Do not operate a device that is damaged CAUTION STOP Verify the scope of delivery when unpacking the device and check for instead contact your local dealer 2 2 Overview of the VIAFILL 2 2 4 Front view 2 3 4 1 Source bottle with source tubings and weight 2 Pump head with rotor and inserted dispensing cassette i 7 Pipetting arm with dispensing cartridge Touch screen display Plate deck with plate mover Waste tubing Wa
16. source con tainer Set the mixing volume and the number of cycles and press enter Mix Wells Mixes the sample that is dispensed to each column Set the number of cycles to mix each well and press enter Blow Out The blow out is performed before the diluted sample is aspirated Select the blowout option to increase the accuracy of the dilution The recom mended Blow Out volume for the 300 ul GripTip Kit is 10 ul and for the 125 ul GripTip Kit is 1 0 ul Purge Last Aspirate Dispenses the aspiration after the last dilution to the reservoir position If turned off the last aspiration is dispensed back into the last col umn that was diluted Sample wait Wait time between each sample dis pense Aspirate wait Wait time after the sample dispense Dispense wait Wait time after each dispense Press SAVE to continue 6 Select the speeds Press the Speed button in the SERIAL DILUTE menu PUMP SPEED SERIAL DILUTE 97 Select the desired dispense speed s and press SAVE DISPENSE SLOW MEDIUM FASI ASPIRATE SLOW MEDIUM FAST MIX SOURCE SLOW MEDIUM FASI MIX WELLAS SLOW MEDIUM FAST www integra biosciences com 35 VIAFILL Operating instructions V02 Operation 7 Adjust the tip height Press the Tip Height button in the SERIAL DILUTE menu TIP HEIGHT SERIAL DILUTE PLATE CLEARANCE 64 8 ASPIRATE MIX SAMPLE 17 6 DISPENSE 41 0 ASPIRATE MIX IN WELL _41 0 BELOW OUT 50
17. standard dispensing cassettes GripTip kit The Reagent Reservoir should be selected using the GripTip pipette tip option Press SAVE to continue 3 Select the tubing set Press the button below TUBE SET in the selected pipetting menu Select the tubing set that is currently installed in the VIAFILL see Dispensing cassette on page 11 and press SAVE TUBING SET 97 Dispensing cassettes use Lai The 16 Channel tubing sets are greyed out if the current dans la lt destination plate is a 96 well plate Select a 384 well plate a t D to allow the selection of 16 Channel tubing sets GripTip kit Select the 300 ul 8 Channel Pipet Tip tubing set or the 125 ul 16 Channel Pipet Tip tubing set Longe Choemel 16 Qhennel 4 Select which wells to fill Press the Plate Map option in the selected pipetting menu PLATE MAP 96 Well Plate J Press the YES and NO buttons to select and deselect em m m mm columns Press the ALL WELLS button to select or WU UN WEN X deselect the entire plate 906000000000 Aves NU 5 swe Press the SAVE button to continue 30 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 Select dispense volume s Press the Volume button in the selected pipetting menu REPEAT DISPENSE J Press the DISPENSE Volume input field to display the s keypad Enter the desired volume and press ENTER DISPENSE The volume being del
18. the dispensing cassettes not get damaged When operating with small bore dispensing cassettes CAUTION STOP Handle the dispensing cassettes with great care so that the tubes and tips ensure that the liquid does not contain any particles gt 50 um For a break of one day or longer empty the tubings by pressing the Recover button Wash the cassette by priming it with deionized water or if necessary with washing detergent followed with deionized water Ensure that all tubings are washed properly Empty the tubings by pressing Recover The cassette can be dried at room temperature Press the green PUSH button 23 on the front of the pump head 2 to lower the housing Release the tubing tension by pressing the silver lever 23 into the down position CAUTION STOP The tubing tension should be released whenever the VIAFILL is not in use 6 2 Cleaning the VIAFILL WARNING Always turn off power and unplug the power supply before cleaning the exterior Spilt fluids can damage the outer surface and internal components For reliable daily operation keep the VIAFILL free of dust and liquid spills Clean the external components periodically with a moistened lint free cloth or Kim wipe lightly soaked with mild soap solution in distilled water Also a 70 dilution of Isopropyl or Ethanol alcohol mix can be used The materials used on the exterior of the VIAFILL support regular cleaning intervals Immediately wipe away spilt acids solvents
19. 0 LO Plate Clearance The height the pipet tips move over the plate when moving from well to well Mix Sample This is the height that the pipet tips mix the sample in the reservoir or in the microwell plate before serial dilute is initiated Set the Mix Source height in conjunction with the Mix Source volume to ensure no air is aspirated into the pipet tips see point 5 Dispense The height of each column dispense This height can be set within the microwell plate Aspirate Mix In Well This is the sample aspiration and mix height within each well Bow Out The height of blow out before the diluted sample is aspirated For Teaching procedure see Teaching procedure for ALL FUNCTIONS on page 33 36 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 3 4 Wash Select one of the WASH options Direction and Rinse within the Toolbox see Wash optional on page 19 Adjust the direction of the aspiration nozzles move or set the Rinse frequency and duration Select the WASH function from the MAIN MENU and set the desired parameters 1 Select the wash mode Press the Wash Function button in the WASH menu and select which wash function to perform FUNCTION 9 Aspirate Only aspirates from wells No well wash is performed in the Aspirate mode Washing Wash module will dispense wash fluid to the entire plate and then aspirate the fluid from the entire plate Wash Aspirote
20. 03 578 5800 F 41 81 286 95 33 F 1 603 577 5529 info integra biosciences com www integra biosciences com Customer service Please contact your local INTEGRA Biosciences AG representative To find out name and address of your local representative go to www integra biosciences com Further information and operating instructions in other languages are available from info integra biosciences com 6 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Introduction 1 Introduction These operating instructions contain all the information required for installation operation and maintenance of the VIAFILL 1 1 Symbols used The operating instructions specifically advise of residual risks with the following symbols WARNING This safety symbol warns against hazards that could result in injury It also indicates hazards for machinery materials and the environment It is essential that you follow the corresponding precautions CAUTION This symbol cautions against potential material damage or the loss of data in a microprocessor controller Follow the instructions gt NOTE This symbol identifies important notes regarding the correct operation of the device and labour saving features 1 2 Intended use The VIAFILL has been designed for use in a laboratory The VIAFILL is a rapid reagent dispenser plate washer pipettor and serial dilutor in the volume range of 0 5 9 999 ul using a variety of tubing se
21. 104s 255s 437s 16 Channel small bore cassette 1536 well 176s 30 5s NA NA 8 Channel 300 ul GripTip Kit 96 well NA 202s 274s 448s 8 Channel 300 ul GripTip Kit 384 well NA 455s 99 9s 1717s 16 Channel 300 ul GripTip Kit 384 well 5 6S 76s 1518S 2385s Dispense speed fast Dispense direction straight Mode Repeat dispense 7 4 Dead volumes for dispensing cassetes E Dead volume Dead volume Dispensing cassette Part No per tube per cassette 8 Channel small bore 5722 200 ul 1 60 ml 8 Channel standard bore 5724 860 ul 6 88 ml 16 Channel small bore 5742 200 ul 3 2 ml 7 5 Specification of the stacker optional Dimensions footprint W x D 78 9 cm x 32 1 cm 31 06 x 12 64 Base unit weight 27 kg Power requirements 100 240 VAC 50 60 Hz 25 Plate chimney height 7 2 cm 50 Plate chimney height 10 2 cm Interface cable RS232C Detection method Optical sensor 50 INTEGRA Biosciences AG VIAFILL Operating instructions V02 7 6 Accuracy and Precision Specifications Dispensing cassettes Technical Data Volume Accuracy Precision Recommended 8 channel 100 ul 1 5 lt 1 0 1000 small bore 2 0 ul 3 0 lt 4 0 plates 8 channel 200 ul 1 5 lt 1 0 2000 standard bore 5 0 ul 2 0 lt 3 0 plates 16 channel 100 ul 1 5 lt 1 0 1000 small bore 2 0 ul 3 0 lt 4 0 plates GripTip tubing cassettes Volume Accuracy Precision Recommended 8 channel 300 ul 2 0 lt 1 5 GripTip
22. INTEGRA INTEGRA j cms INTEGRA VIAFILL VIAFILL Operating instructions 900 00006 00 V02 INTEGRA Declaration of conformity Konformitatserklarung C D claration de conformit Declaraci n de conformidad Dichiarazione di conformit INTEGRA Biosciences 2 Wentworth Drive Hudson New Hampshire 03051 declares on its own responsibility that the product erkl rt in alleiniger Verantwortung dass das Produkt d clare sous sa responsabilit exclusive que le produit declara bajo su propia responsabilidad que el producto dichiara sotto la propria responsabilit che il prodotto VIAFILL Dispenser Model 5600 VIAFILL Plate Stacker Model 5910 in accordance with EC directives gem ss der EU Richtlinien est conforme au terme de la directives CE de acuerdo con las directivas CE in conformit alle direttive CE 2006 95 EC Low voltage equipment 2004 108 EC Electromagnetic compatibility 2002 95 EC Restriction of Hazardous Substances 2002 96 EC Waste Electrical and Electronic Equipment is in compliance with the following normative documents mit den folgenden normativen Dokumenten bereinstimmt aux documents normatifs ci apr s cumple las documentos normativos soddisfa le normative seguenti EN 61010 1 Safety requirements for electrical equipment for measurement control and laboratory use General requirements EN 61326 1 Electrical equipment for
23. Wash module The VIAFILL can be converted from a reagent dispenser to a plate washer by attaching the wash tubing set and external wash module This allows users to wash plates and aspirate the waste to an external waste container 4 1 1 Materials included Unpack the wash module and confirm the contents These include 1 x main wash module tower 1 x large waste container 1 x waste container lid 2 x small rinse and wash fluid containers 5 x pieces of silicone tubing 3 short 1 long 1 wide bore tubing 1 x power cable 1x RS232 communication cable NOTE The wash tubing set 8 channel is part no 5960 16 channel is part no 5965 needs to be ordered separately 4 1 2 Description of the wash module 31 32 39 d 35 36 37 38 www integra biosciences com Wash tubing W connected to wash container Rinse tubing R connected to rinse container Wide bore air tubing A connected to waste container lid Long waste tubing A fitted end connected to waste container lid other end to wash tubing cassette head clamped on the top of the wash module Wash and Rinse containers Wash module Nozzle tubing N connected with wash tubing cassette Waste bottle 21 VIAFILL Operating instructions V02 Installation of optional accessories 4 1 3 Wash module set up 9 C3 E 2 3 D 22 Place the wash module tower next to the VIAFILL Plug the power cable into the bac
24. ad on the 5823 base unit 16 channel GripTip kit enables use of 125 ul GripTips 5840 includes 5841 5842 5823 16 channel tubing set to connect 16 channel pipetting head 5841 16 channel pipetting head for the use of 125 yl GripTips 5842 GripTip adaptor for the installation of the pipetting head on the base 5823 unit GripTips Part No 125 ul GripTips 5 inserts of 384 tips non sterile GREEN CHOICE 4422 5 boxes of 384 tips non sterile 4423 5 boxes of 384 tips sterile 4424 5 boxes of 384 tips sterile filter 4425 5 boxes of 384 tips pre sterilized GREEN CHOICE 4426 300 ul GripTips 5 inserts of 96 tips non sterile GREEN CHOICE 4432 5 boxes of 96 tips non sterile 4433 5 boxes of 96 tips sterile 4434 5 boxes of 96 tips sterile filter 4435 5 boxes of 96 tips pre sterilized GREEN CHOICE 4436 www integra biosciences com 53
25. ar paths within each well Dispensing cassette The standard dispensing cassettes or the wash tubing set work as a flow through system Only a Bottle can be utilized as a source container because they do not have the ability to aspirate from a reservoir or a plate GripTip kit The GripTip pipette dispensing option is not a flow through system The liquid must be aspirated separately The GripTip kit only functions with a Reagent Reservoir or a Plate source options www integra biosciences com 29 VIAFILL Operating instructions V02 Operation 5 3 2 Repeat and variable dispense Select the REPEAT DISPENSE or VARIABLE DISPENSE function from the MAIN MENU and set the desired parameters 1 Select the plate that is located on the deck Press the Plate option in the selected pipetting menu PLATE SEECTON Match the plate on the screen to the plate that is seated CO mmm on the VIAFILL plate deck After selecting the correct 5 plate press the SAVE button See section 3 2 2 to see what tubes or which pipet tips to disconnect to dispense to a 6 12 24 or 48 well plates 2 Select the source container Click on a SOURCE option in the selected pipetting menu SOURCE 9 Dispensing cassettes 3 j p Only the Bottle can be utilized with the standard 8 dispensing cassettes Selecting the Plate or Reagent TI amp mstfewsrfse Reservoir option will disallow the selection of the A
26. c e mem p oTe 7 8 9 n uo 2000 Ca sxe Press the SAVE button to continue 6 Select dispense volume s Press the Volume button in the WASH menu VOLUME WASH Dispense This is the volume of wash fluid that is dis osese Liang 7 wie pensed to each well The aspiration volume is auto ASPIRATE L1 50 matically calculated and performed after each WASH CYCLES EI dispense ESES wash cycles Number of wash cycles for column or plate wash Wait sec Wait time before each dispense or aspira tion Press SAVE to continue 7 Select the speeds Press the Speed button in the WASH menu PUMP SPEED SERIALDILUTE Select the desired dispense speed s and press SAVE DISPENSE SLOW MEDIUM FASI ASPRATE SLOW MEDIUM EASL MIX SOURCE SLOW MEDIUM FASI MX WELLAS SLOW MEDIUM EASI a c3 38 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 8 Adjust the tip height Press the Tip Height button in the selected pipetting menu TIP HEIGHT WASH e PLATE CLEARANCE 23 7 ASPIRATESTART 15 4 DISPENSE L16 S ASPRATE 11 6 PRE DISPENSE ullo Plate Clearance The height the Wash aspiration nozzles move over the plate when moving from col umn to column Dispense The height that the dispensing cassette parks while the wash fluid is dispensed Pre Dispense The height that the Wash tubing cas sette dispenses over the waste
27. ccccseseeeseeeeeeneeseeeeeeeseeeeeneeeesscees 27 4 3 2 Description of the external mixing pump 27 4 3 3 Mixing pump set up eee m 27 Operation 5 1 Turm on the device eeseeeeeneeeeeeenenennennnme nnne 28 5 2 Quick start Quide E A A AEAT elites 28 5 3 Pipetting functions 29 5 3 1 Overview e deer U Ue E 29 5 3 2 Repeat and variable dispense sseeeess 30 5 3 3 Serial dilute n earn 34 cw MM Ec ia 37 5 4 Special functions dispensing cassette only 40 5 4 PETI ar tdt ce PD certe ote eid 40 5 4 2 RECOVER a e D ed go 40 5 5 PrOUfatms uitae iei ERE RPM HIRED 41 5 5 Save ai program ee ete Deas er 41 5 5 2 GODy a PFOQKAIN s 2er tera ERR EK Re RRS in 41 5 5 3 Custom programs 0 n Lane etti REN 41 5 5 4 Run a program x EE aiiin 42 5 6 Stacker function optional eene 43 5 6 1 Stacker program modes sseseeee 43 5 7 Troubleshooting FAQ seen 44 5 7 1 VIAFILL base unit sesessseeeneenenenen 44 5 7 2 SLACK Chats nomeo dte eec Eh D eerie i dtt 45 5 7 9 Wash6erz eid tee ee ENN 45 Maintenance 6 1 Washing the dispensing cassettes eeeeeees 46 6 2 Cleaning the VIAFILL eeseeseeeeeeeeeenneennneennennnnnns 46 6 3 Decontamination of the VIAFILL
28. d Two plate chimneys are available with the capacity of 25 plates or 50 plates 4 2 1 Materials included Unpack the plate stacker and confirm the contents These include Stacker base unit Power cable Serial communication cable Waste tubing Chimney hardware 2 support brackets 4 hexagon head screws and 4 washers 2 clips rubber pad NOTE Stacker Chimneys sold separately from base unit Two Chimneys must be ordered to use the stacker properly Two individuals are required to lift the stacker base unit onto a level bench www integra biosciences com 23 VIAFILL Operating instructions V02 Installation of optional accessories 4 2 2 Description of the stacker Front view 39 J40 li 39 40 41 42 43 44 j 42 43 44 Back view e s 45 ae 4 ji T 47 C Front panel 48 48 49 50 50 4 2 8 Stacker set up VIAFILL base unit not included Plate chimney position 1 not included Plate chimney position 2 not included Waste tubing Stacker base unit Stacker ON OFF switch Fuse RS232 Port for VIAFILL Power supply port Arrow keys to adjust the plate size Re stack to restack the plates back to the first chimney position Lids to remove the lids before dispensing 1 Put the VIAFILL into STACKER mode Turn the VIAFILL ON and press the red TOOLBOX icon in the upper right corner Press Stacker in the TOOLBOX main menu to turn the stacker ON see Stacker optional on page 20 This must b
29. d dispensing cassette installed press PRIME until liquid is dispensed from tips 7 Press the green RUN button to start 8 Before prolonged standby wash the cassette and store it in the rest position see 6 1 9 Turn off the VIAFILL 28 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 3 Pipetting functions 5 3 1 Overview The VIAFILL provides the following pipetting functions depending on the dispensing cassette installed Within the table the source container is listed Function oe GripTip kit Hse s t Repeat dispense Bottle Reagent reservoir NA Variable dispense Bottle Reagent reservoir NA Serial dilute NA Reagent reservoir or plate NA Wash NA NA Bottle REPEAT DISPENSE The function is designed to rapidly fill a micro plate from a source container VARIABLE DISPENSE The function is designed to rapidly fill differing volumes to a micro plate from a source container The volumes can be programmed for each individual column SERIAL DILUTE The function for the GripTip adaptor is designed to rapidly dilute a microwell plate from the reagent reservoir or from a selected plate column WASH The module allows users to wash plates and aspirate the waste to an external waste container Also a timed rinse can be programmed to rinse remaining waste from the tubing set To enhance the efficiency of the aspiration a choice of aspiration patterns can be set in the Toolbox for linear or circul
30. e and press Save www integra biosciences com 41 VIAFILL Operating instructions V02 5 5 4 Run a program REPEAT DISPENSE Plate vnction Repeat Dispense ote Map Partial Plote ume peec vbe Set 7 LB Platsic Lg 224 Fast fave Plate shaker MAIN LREPEATDISPENSE 4 WASH 2 VARIABLE DISPENSE 5 TOM Tele lt lt 3 SERIAL DILUTE 42 Operation After the parameters are set press the green RUN to start the repeat dispense Press the STOP button at any time to pause the program Once the program is paused press CONTINUE to resume or END to cancel the program The ability to SHAKE microwell plates in available on all VIAFILL base units Shaking plates helps to homogenize the reagent while simultaneously reducing air bubbles within the reagent Simply hold down the SHAKE button to shake plates The SHAKE button is located on the VIAFILL main menu and in all function main menus Repeat Dispense Variable Dispense Serial Dilute and Wash INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 6 Stacker function optional The VIAFILL plate stacker allows for microwell plates to be stored dispensed to and then restacked Press the HOME button to sync the stacker and the VIAFILL The Stacker is designed to work with the REPEAT DISPENSE and VARIABLE DISPENSE functions Select either of these or a Custom program to begin Use the GripTip kit with the stac
31. e completed before connecting the stacker Turn the VIAFILL OFF before continuing INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation of optional accessories 2 Remove the VIAFILL reservoir and stage Simply slide the reservoir holder out of the VIAFILL base Three cross slot screws a need to be removed on the underside of the plate deck 5 This will allow the entire plate deck to be removed a 3 Slide the VIAFILL onto the stacker Slide the VIAFILL from the back onto the stacker base unit 43 The VIAFILL feet will fit into four holes b on the deck of the stacker b 4 Insert the reservoir Attach the waste tubing to the reservoir and tighten the 2 screws c with a hexagon wrench 5 Asseble the chimneys Assemble stacker chimney support brackets with 2 hexagon head screws and washers each d on stacker base by using hexagon wrench www integra biosciences com 25 VIAFILL Operating instructions V02 Installation of optional accessories Connect the stacker chimneys by lowering them over the pins e on the chimney support brackets 6 Attach the clips and the pad Attach the two clips f to secure the VIAFILL to the stacker Place the adhesive back of the rubber pad on the stacker 1 rail where the pipetting arm track meets the stacker rail f 7 Connect the cables Use RS232C cable to connect the VIAFILL and Stacker Base Connect the RS232C
32. erature fluctuations or direct sunlight Place the VIAFILL on a flat dry clean and vibration proof bench Leave at least 10 cm 3 9 in space on both sides and on the back to allow adequate air circulation 3 2 Assembling the instrument Unpack the VIAFILL from the packaging configuration Attach the power cord Ensure that the mains switch 8 on the back panel is in the OFF position 3 2 1 Installing the dispensing cassettes Different dispensing cassettes GripTip kits or wash sets can be used with the VIAFILL The standard dispensing cassette is designed for rapid reagent addition using the Repeat Dispense and Variable Dispense functions The standard dispensing cassette does not function with the Serial Dilute or Wash functions The VIAFILL GripTip option is designed for the Repeat Dispense Variable Dispense and Serial Dilute functions b a 1 Press the green PUSH button a on the front of the pump head 2 to lower the housing and reveal the rollers that the tubing must be clamped against 2 Press the silver lever b into the down position This will allow the dispensing cassette to be loaded Slide the dispensing cassette under the rollers of pump head Fit the two holes on the inside of the left black cartridge 25 with the two silver pins c located on the sliver lever Position an equal number of tubes on either side of the black roller wheel 22 12 INTEGRA Biosciences AG VIAFILL Operating instructions
33. f the following procedures Rinse the tips by pressing the Recover and the Prime button alternatively for a few seconds Fill the reagent reservoir with deionized water and let the tips submerged for a few minutes Press the Recover button Dismount the dispensing cassette Fill a 20 ml syringe with deionized water or ethanol attach a round sterile filter and put a suitable short tubing on the syringe and on the outlet of the tip Press liquid through the syringe To verify the cleaning put the tubing to the tip inlet and check if liquid is coming out of the tip while pressing liquid through 6 6 Servicing It is recommended to service the VIAFILL at least yearly If liquid ever enters the internals of your VIAFILL please contact INTEGRA Biosciences US for service advice A WARNING A If working with infectious materials e g human pathogens VIAFILL needs i tobe decontaminated before sending them to service and the declaration on the absence of health hazards must be signed This is necessary to protect service personnel www integra biosciences com 47 VIAFILL Operating instructions V02 Maintenance 6 7 Calibration The VIAFILL and each tubing set is calibrated by the manufacturer to ensure that each channel is accurate You can gravimetrically determine the accuracy of an entire dispensing cassette i e the ability of the pump to dispense the exact volume desired Weigh a micro plate and dispense distilled water int
34. f the text is white and not available if the text is grey 10 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Description of the device 2 2 4 Pump head 22 23 22 Pump rollers peristaltic pump rotors where cartridge tubings are stretched against 23 Green PUSH button to lower the housing of the pump rotor 24 Silver lever down position for loading of dispensing cassettes and to release the tubing tension rest position 24 2 2 5 Dispensing cassette Left tubing cartridge with two holes inserted on the silver lever Right tubing cartridge placed in the groove on the pump head Dispensing cartridge inserted on the holder of the pipetting arm Expression on display Dispensing cassettes Part No Large 8 channel Plastic Tip 8 channel standard bore 5724 Small 8 channel Plastic Tip 8 channel small bore 5722 16 channel Plastic Tip 16 channel small bore 5742 8 channel Washing 8 channel wash set 5960 16 channel Washing 16 channel wash set 5965 300 ul 8 channel Pipet Tip 8 channel tubing set for GripTips 5821 125 ul 16 channel Pipet Tip 16 channel tubing set for GripTips 5841 Stainless Tips or Metal Tips are not available yet www integra biosciences com 11 VIAFILL Operating instructions V02 Installation 3 Installation 3 1 Operating environment The VIAFILL has been designed for use in a laboratory It shall be operated in a dry and dust free location without large temp
35. ily turned ON or OFF in the menu Dispense Touch Off This is the height that the pipet tips touch the dispensed reagent to eliminate any remaining reagent on the end of the pipet tips This is performed at the end of each column dispense Pre Dispense Touch Off This is the height that the pipet tips touch the reservoir reagent after the pre dis pense Turn ON to eliminate any remaining reagent on the end of the pipet tips Aspirate This is the reagent aspiration height from the reservoir The overall dispense volume should be considered to ensure the aspirate height is deep enough with the reagent reservoir to accommodate the total of all dispenses Mix Source This is the height that the pipet tips mix the reservoir reagent before the repeat dispense begins Set the Mix Source height in conjunction with the Mix Source volume to ensure no air is aspirated into the pipet tips INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation Teaching procedure for ALL FUNCTIONS Press the height button mm to open the tip height teach screen The current TIP HEIGHT is displayed on the left of the screen TIP HEIGHT TEACH G Press the MOVE PLATE button titled COLUMN 1 to TIP TEACH NEW MOVETIP MOVE PLATE HEIGHT HEIGHT move couww Move the plate deck under the dispensing cassette ILo 0300 Tip sFeED HOME cartridge SLOW CY 000 Use this position to set the heights The MOVE PLATE v 5 E33 HOME b
36. ivered to each well VOLUME REPEAT mtl EET m 9 J GripTip kit only DISPENSE Lig 77 veed DISPENSE The volume being delivered to each well septa Ea w PRE DISPENSE This volume cannot be adjusted mix source MSN aoo Ls Ps save This volume is used to increase accuracy and preci sion and also to set the peristaltic pump in the correct position The predispense volume for the 300 ul tub ing set is 20 ul and for the 125 ul tubing set is 4 ul ASPIRATE The volume that is aspirated For aliquot dispensing set the volume by a multiple of dispense volume MIX SOURCE The volume that the pipet tips aspirate and dispense into the reservoir before the Repeat Dispense begins The Mix Source number of Cycles can also be set and turned On or Off YES or NO A pause wait sec can be set and adjusted This pause is between each aspirate and dispense Press SAVE to continue VARIABLE DISPENSE y Only the wells that were selected as YES in the PLATE MAP screen are allowed to be edited Press the column J volume indicator to activate the volume keypad as shown l here Enter the desired volume for each column and press ENTER Press SAVE to continue For filling less than 96 well plates some tubes need to be removed from source see Tubing selection for 6 through 48 well dispensing on page 15 In addition the corresponding columns have to be deactivated in the plate map function in order to obtain a co
37. k of the wash module Connect the RS232 cable from the VIAFILL to the wash module Place short tubing R into the small rinse container and connect the other end to the wash module port labeled RINSE Place short tubing W into the small wash container and connect the other end to the wash module port labeled WASH Connect wide bore air tubing A to the wash module port labeled AIR Connect the other end of the wide bore air tubing A to the waste container lid either port Connect the long waste tubing end with fitting A to the waste container lid second port INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation of optional accessories 9 Press the middle of the long tubing labeled A into the pump head on the top of the wash module Do not connect the other end yet 10 Connect the small tubing N into the wash module port labeled NOZZLE Do not connect the other end yet 11 Load either the 8 channel wash tubing cassette 5960 or 16 channel wash tubing cassette 5965 e onto the VIAFILL See section 3 2 1 for instructions on how to install cassettes 12 Connect other end of long tubing labeled A to the amp top of the wash tubing cassette head 13 Connect the other end of the nozzle tubing N to the nozzle on the end of the wash tubing cassette 4 2 Plate stacker The VIAFILL plate stacker allows for microwell plates to be stored dispensed to and then restacke
38. ker when in plate serial dilutions should be performed The Stacker will not function if used with the Wash tubing set If the stacker intended to use as a bulk reagent dispenser install the standard dispensing cassettes Only the Bottle can be utilized as source container The VIAFILL stacker is also capable of stacking plates with lids and deep well blocks Simply adjust the tip height within the repeat or variable dispense menu to use the stacker with plates of differing heights The stacker plate counter is shown on the right side of the Repeat Dispense and Variable Dispense screens CLR pei E ip HeTohi Rej c Dupe ense ii Well Bottle Partial Plate men ig a Volume Speed 3 Plas T3 3 Plastic Lo 224 ul 100 a J amp c tj 5 6 1 Stacker program modes Lid stack Press the LIDS option on the front panel of the stacker 50 to activate the plate lid function This will remove lids from each plate store it dispense to the plate and then replace the lid before stacking Turn this function ON or OFF at any time by pressing the button Use the arrow Keys are used to adjust the plate height The plate height is measured from bottom to top without lid or from bottom of plate to bottom of lid for lidded plates Plate Re stack Press the RE STACK button on the front panel of the stacker 49 to activate the plate restack function After filling all plates the stacker will then re stack the plates back to the
39. lder f is installed unscrew the two screws g on the pipetting arm and Jl remove the holder 2 If the GripTip adaptor is installed unscrew the two screws f on the pipetting arm and remove the adaptor Place the standard cassette holder on the pipetting arm and fix it beginning with the frontmost Screw 3 2 3 Tubing selection for 6 through 48 well dispensing Just disconnect the appropriate tubing from the source tubing weight 1 to adapt the tubing to dispense liquid into 6 12 24 and 48 well plates NOTE 6 12 24 and 48 well plates can only be dispensed to using the standard dispensing cassettes The VIAFILL GripTip kits cannot dispense to 6 12 24 and 48 well plates Below is an instructional diagram for use of 8 channel cassette tubing sets on 6 12 24 and 48 well plates Simply attach the standard 8 channel cassettes 5722 amp 5724 and disconnect the tubes shown below Tube one is closest to the instrument Select the desired program select the correct 8 channel tubing set and a 96 well plate 6 Well Plate 12 Well Plate 24 Well Plate 48 Well Plate disconnect disconnect disconnect disconnect disconnect disconnect disconnect disconnect disconnect Tube4 disconnect Tube5 disconnect disconnect disconnect disconnect disconnect disconnect Tube 8 disconnect www integra biosciences com 15 VIAFILL Operating instr
40. nal First select the pump speed Turn ON the interval time and press the time button to set the amount of time between each rinse Select the rinse time button to set the duration of the rinse Also the rinse button can be held down to perform an immediate rinse Press SAVE to continue The VIAFILL plate stacker allows for microwell plates to be stored dispensed to and then restacked TOOLBOX STACKER e PAUSE AFTER STACKER OFF SERIAL DILUTE L EEn UNLOAD HOME OFF 3 4 Help screens Press the Stacker in the TOOLBOX main menu to turn the stacker ON and OFF Turn UNLOAD HOME ON and the carrier of the stacker will restack the plate to unload the stacker at the beginning of the run The stacker will confirm there are no plates on the carrier Turn PAUSE AFTER SERIAL DILUTE ON when using the GripTip kit to perform multiple in plate serial dilutions using the stacker After each plate the VIAFILL will pause to allow the user to eject GripTips and load new GripTips Only in plate serial dilutions can be performed using the stacker The sample cannot be aspirated from the reservoir when using the GripTip kit Press SAVE to continue Press the blue question mark on any screen to access the Help Text The Help Text explains each screen in detail 20 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation of optional accessories 4 Installation of optional accessories 4 1
41. o the first column using the repeat dispense function Weigh the plate again and calculate the weight of the actual volume Repeat this procedure with a calibrated pipette and calculate the weight of the target volume Determine the percent deviation of the actual to the target weight and adjust the calibration factor see Pump calibration on page 19 if necessary The VIAFILL can also be calibrated by your service technician This includes gravimetric accuracy verification photometric precision verification and gravimetric calibration Please contact INTEGRA Biosciences Corporation 6 8 Equipment disposal The VIAFILL is labelled with the crossed out wheeled bin symbol to indicate thatthis device must not be disposed of with unsorted municipal waste Instead itis your responsibility to correctly dispose of your waste equipment by handing it over to an authorised facility for separate collection and recycling It is also mmm your responsibility to decontaminate the device in case of biological chemical and or radiological contamination so as to protect from health hazards the persons involved in the disposal and recycling of equipment For more information about where you can drop off your waste equipment for recycling please contact your local dealer from whom you originally purchased the product or your local council By doing so you will help conserve natural resources and you will ensure that your waste equipment is recycled in
42. reservoir before beginning the Wash This function assures that there is no air in the dispensing tubing set Aspirate Start This is the height that the aspiration nozzles start aspirating the wash fluid from each well Set this height slightly below the height of the Wash volume for each well Aspirate This is the height that the aspiration noz zles stop aspirating the wash fluid from each well Set this height to the bottom of the microwell plate For Teaching procedure see Teaching procedure for ALL FUNCTIONS on page 33 www integra biosciences com 39 VIAFILL Operating instructions V02 Operation 5 4 Special functions dispensing cassette only 5 4 1 Prime NOTE This function is only available with standard dispensing cassette The GripTip pipet tip tubing set does not require system fluid The GripTip tubing sets function correctly with no liquid column As a result the user interface will not allow the PRIME function to be used when the GripTip tubing set is selected Before priming the dispensing cassette set the prime height in the TOOLBOX see Prime purge on page 16 A prime needs to be performed before the standard dispensing cassettes can be used for dispensing The prime function draws the bottle source fluid through the tubing sets and prepares the tubing set for dispensing REPEAT DISPENSE E SIN Enter the MAIN screen for the method being performed Repeat Dispense 96 Well 240m Repeat Dispen
43. ress MOVE TIP to move the pipet tips s to the new tip height value or alternatively to the upmost onaz position Press SAVE to continue ENIER 3 3 2 Mix pump optional The external mixing pump allows users to keep cells in suspension while simultaneously dispensing to microwell plates Select the MIX PUMP option from the TOOLBOX TOOLBOX MIX PUMP Turn the external mixing pump ON or OFF by pressing MIX PUMP the YES NO button The interval button defines how INTERVAL 60 sec many seconds the pump runs in each direction The TIMER 30 minutes Timer defines the total time the pump will run in intervals until shutting off 3 3 3 Pipet direction The function only applies when using an eight channel tube set to dispense to a 384 well plate or when using a sixteen channel tube set to dispense to a 1536 well plate The plates are filled in two steps depending on the defined pipetting direction TOOLBOX PIPET DIRECTION 9 STRAIGHT first every other row A C E is SEIS dispensed starting from the first column and then the o pipetting arm shifts sideways to fill the remaining rows B D F starting from the last column STAGGER every column is dispensed in two steps starting from the first column with every other rows A C E and then followed by the remaining rows B D F Select the preferred pipetting direction and press Straight Stagger SAVE to continue
44. robable cause The stacker does not The stacker option was not communicate with activated in the toolbox the VIAFILL The RS232 cable is not connected properly Operation Remedy Got to the stacker option in the red toolbox Turn the stacker option ON Restart both the VIAFILL and Stacker Connect the RS232 cable as described in section The lid function is Incorrect lid height entered not working properly into plate lid height option on the front stacker panel Enter the correct height in mm from the bottom of the well plate to the bottom of the lid The RUN button will The plate counter has reached not activate the the limit 999 VIAFILL Stacker Reset the plate counter 5 7 3 Washer Problem Probable cause Remedy The Wash Module The Wash mode might not be In the function screen select will not run selected Aspirate Washing or Column Washing The wash module will not operate unless one of these is selected The wash tubing Installation error See section 4 1 3 for installation cassette is not dispensing aspirat instructions or contact a service technician for a detailed installation ing guide The wash tubing No vacuum created in waste Make sure plastic cap is on the cassette is not container waste container lid opening not in aspirating use by the two tubes www integra biosciences com 45 VIAFILL Operating instructions V02 Maintenance 6 Maintenance 6 1 Washing
45. rrect and clean filling www integra biosciences com 31 VIAFILL Operating instructions V02 6 Select the speeds Operation Press the Speed button in the selected pipetting menu PUMP SPEED SERIAL DILUTE e DISPENSE SLOW MEDIUM FASI ASPRATE SLOW MEDIUM EASL MIX SOURCE SLOW MEDIUM FASI MIX WELLAS SLOW MEDIUM FASI 7 Adjust the tip height Select the desired dispense speed s and press SAVE Press the Tip Height button in the selected pipetting menu IP HEIGHT REPEATAVARIABLE DISPENSE BOTTLE mm n PLATE CLEARANCE _ 30 0 20 0 0 0 DISPENSE PRE DISPENSE TIP HEIGHT REPEAT SVARIABLE DISPENSE PIPET TIPS m PLATE CLEARANCE 60 0 ASPIRATE 28 0 DISPENSE 55 0 PRE DISPENSE 45 0 DISPENSE TOUCH Orr 20 52 0 MXSOURCE 28 0 PRE DISPENSE ON 30 0 TOUCH OFF 32 Dispensing cassettes The plate clearance dispense and pre dispense heights can all be easily adjusted Plate Clearance The distance the dispensing cas sette parks over the plate when the plate is moving between wells Dispense The height of each column dispense This height can be set within the microwell plate Pre Dispense The height the dispensing cassette dispenses to the waste container before dispensing to the plate GripTip kit The GripTip tubing cassette allows for additional tip heights to be set The Touch Off heights can be eas
46. s BACK to continue 3 3 8 Recover The recover function reverses the peristaltic pump direction to move the liquid in the tubing back in to the source container This function is only available for the standard tubing casettes TOOLBOX f RECOVER eJ RECOVER speeds can be set for the different tubing Tubing Set Speed Position m masne nes SLOW MEUM fast Lses Sets METAL TIPS SLOW MEDIUM FASI 0 1 WASH TUBE SLOW MEDIUM LEAST Cos Press the Position mm button to adjust the height that rs save the RECOVER will be performed at see 3 3 1 18 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation 3 3 9 Pump calibration Each tubing sets factory setting is 1 00 100 percent To change the calibration factor simply press the value next to the desired tubing set Pump Calibration eJ Use the keypad to adjust the calibration factor To 18 Plosticig 1 00 616See 1 00 increase the volume by one percent press 1 01 and 28Sedig 1 00 78Weh 1 00 3 8 PlowicSm 1 00 8 16wesh 1 00 press ENTER 4 8 Stael Sm 1 00 93004Tip 1 00 5 16Plowie 1 00 10125uTip 1 00 Press SAVE to continue 3 3 10 Wash optional TOOLBOX il WASH 9 The two Wash options are Direction and Rinse DIRECTION RINSE Press the Direction button to adjust the direction the aspiration nozzles move TOOLBOX ray WASH DIRECTION o Select the direction that the
47. s the wash interval and timer to be adjusted Stacker Allows the Stacker to be turned On or Off 3 3 1 A prime purge is performed when pressing the PRIME button in the function menus Repeat Dispense Variable Dispense Serial Dilute and Wash Prime purge TOOLBOX PPRIME PURGE PRIME speeds can be set for the different tubing sets pasne TS SLOW MEDIUM east 15 PLASTIC TIPS METAL TIPS WASH TUBE and PIPET ware aor tan Ha Li TIPS Select the Speed CL MMC Press the Position mm button to adjust the height that the PRIME and PURGE will be performed at 16 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Installation TIP HEIGHT TEACH Te The current tip height setting is displayed on the left of the TIP TEACH NEW MOVE TIP MOVE PLATE HEIGHT HEIGHT MOVE COLUMN 1 Screen ALL z neset nont a Press the MOVE PLATE button titled HOME to move CY nl the plate deck to the far right Use this position to set v E the prime and purge heights b Adjust the tip adjustment speed by pressing the FAST SLOW and CONTINUOUS STEP buttons Press the A UP and W DOWN arrows to adjust the tip height c Press ENTER to select the tip height and press SAVE to continue TIP HEIGHT TEACH HEELS The height can also be entered using a keypad by HEIGHT NEGAT ooo pressing the TIP HEIGHT button Once the numerical eee ws 4 6 fo value is entered p
48. se or Variable Dispense Beit c feelers BASE old the PRIME button shown here until the source fluid is dispensed from all dispensing cassettes nozzles vme Spee Ca fave ag 100 5 4 2 Recover Before using the recover function set the recover height in the toolbox see Recover on page 18 7 4 n The recover function should be used when users are finished using the VIAFILL standard dispensing cassettes This function reverses the pump motion and pulls the reagent back into the source container The recover function is only available in the Repeat Dispense and Variable Dispense modes Enter the Main function screen for the method being performed Repeat Dispense or Variable Dispense Hold the RECOVER buiton until the reagent is completely amp removed from the dispensing cassette REPEAT DISPENSE unction ate Repeat Dispense 96 Well ote Map Source ote Mop Bottle Portial Plate vme gt 40 INTEGRA Biosciences AG VIAFILL Operating instructions V02 Operation 5 5 Programs 5 5 1 Save a program You should save programs as custom programs to maintain the parameter settings especially the tip height values From the Repeat Dispense Variable Dispense Serial Dilute or Wash main menus press SAVE to store the current parameters SAVE AS J Press SAVE AS to save the parameters with a custom Luts name The screen beside will appear Select which amp ou Bo custom program
49. ste amp reservoir station 6 5 N OO 2 o 2 2 2 Back view 8 Main switch 9 RS232 Ports 9a for external mixing pump 9b for washing module 9c for stacker 9d additional port for PC 10 Power supply port www integra biosciences com 9 VIAFILL Operating instructions V02 2 2 3 Display MAIN 2 VARIABLE DISPENSE 5 LREPEATDISPENSE 4 WASH 3 SERIAL DILUTE TOM KS 13 14l 15 16 17 REPEAT DISPENSE Function i yi et Z Plastic LO dead nul ume 3i TI ote Repeat Dispense 96 Well Source ote Bottle Partial Plate Description of the device Toolbox for general settings Help to explain screens Stacker option to stack plates will be highlighted if STACKER is properly connected Mix Pump option to mix source container will be highlighted if MIX PUMP is properly connected Prime Purge button aspirates the source fluid filling the dispensing cassette and pre dispenses the source fluid Hold button until source fluid is dispensed Recover reverse pump movement to recover the liquid from the tubing into the source bottle as long as button pressed Shake to shake the plate linearly as long as button held down Run to start the dispensing protocol Stop to stop the protocol at any time Back to navigate to the previous screen Save as to save the pipetting parameters with a 18 13 20 custom name NOTE The buttons are operational i
50. ts The GripTip pipette tip option allows to utilize the VIAFILL as a serial dilutor It shall not be used for applications other than those specified www integra biosciences com 7 VIAFILL Operating instructions V02 Introduction 1 3 1 2 Safety notes The VIAFILL corresponds to the state of the art complies to the recognized safety regulations and is safe to operate The VIAFILL can be operated only when it is in perfect condition and while observing these operating instructions The device may be associated with residual risks if it is used or operated improperly by untrained personnel Any person operating the VIAFILL must have read and understood these operating instructions and particularly the safety notes or must have been instructed by supervisors so that safe operation of the device is guaranteed Regardless of the listed safety notes additional applicable regulations and guidelines of trade associations health authorities trade supervisory offices etc must be observed Do not open or modify the VIAFILL in any way The back panel must not be removed Repairs may only be performed by INTEGRA Biosciences AG or by an authorised after sales service member Parts may be replaced with original INTEGRA Biosciences parts only AC Inlet If any abnormalities occur such as smoking or ignition immediately disconnect device AC Inlet from power supply Therefore set up VIAFILL where it is easy to insert and remove a
51. u and switch the mix pump on see Mix pump optional on page 17 4 Adjust the speed of the external mixing pump by turning the knob on the front of the pump www integra biosciences com 27 VIAFILL Operating instructions V02 Operation 5 Operation 5 1 Turn on the device CAUTION STOP Remove hands from the VIAFILL at switch on and during homing Turn on the VIAFILL by pressing the main switch 8 that is located on the back panel MAN d J 0 If the VIAFILL starts properly LREPEATDISPENSE 4 WASH Lousron the display lights up 2 VARIABLE DISPENSE 5 ION 8 CUSTON i Soi you are prompted to Press to Home to perform a TERA OiT A homing routine zza the MAIN MENU is displayed as shown beside 5 2 Quick start guide The following quick start list gives you an overview how to start pipetting immediately 1 Insert an appropriate dispensing cassette see 3 2 1 2 Turn on the VIAFILL 3 With standard dispensing cassette installed define the prime and recover heights in the TOOLBOX see 3 3 1 and 3 3 8 Before performing wash plates define the wash parameter in the TOOLBOX see 3 3 10 4 Select one of the pipetting functions from the MAIN MENU 5 Check or change the plate the source container the tubing set which wells to fill the dispense volume the speeds and the tip height see 5 3 Alternatively select a custom function with all parameters stored 6 With standar
52. uctions V02 Installation 3 3 Toolbox adapt your VIAFILL The TOOLBOX is accessible from the Main Menu and the different function screens Repeat Dispense Variable Dispense Serial Dilute and Wash TOOLBOX amp MIX PUMP SOUNDS STACKER Simply press the red TOOLBOX case to access the ERNE FURGE cowwucanon Pump cabrien TOOLBOX MAIN MENU Once in the Toolbox select the STAGE ALIGNMENT WASH PIPET DIRECTION OWNER INFO RECOVER desired function to change its settings a Toolbox options Description Prime purge Allows the height and speed of the prime to be adjusted for each dispensing cassette Mix pump Allows the pump to be turned ON or OFF The interval and timer settings can also be adjusted Pipet direction Choose from Straight and Stagger pipetting directions Sounds Allows beep tones to be turned ON or OFF Communication Turns PC communication capability ON or OFF Stage alignment Allows cassette nozzles to be properly positioned over a well Owner information Displays the serial number software version and display version The owner name can also be modified using this option Recover Allows the height and speed of the recover function to be adjusted for each dispensing cassette Pump calibration Allows to change the calibration factor of the pump Wash Allows the pump to be turned ON or OFF Also allow
53. utton moves the plate deck to the far right a Press the A UP and W DOWN arrows to adjust the tip height Adjust the tip adjustment speed by press ing the FAST SLOW and CONTINUOUS STEP but tons Press ENTER to select the tip height and press SAVE to continue TIP HEIGHT TEACH Wis dass HIT 100 flan T DOO b The height can also be entered using a keypad by ALL A pressing the TIP HEIGHT button Once the numerical EA value is entered press MOVE TIP to move the pipet tips to the new tip height value Press SAVE to continue www integra biosciences com 33 VIAFILL Operating instructions V02 Operation 5 3 3 Serial dilute Select the SERIAL DILUTE function from the MAIN MENU and set the desired parameters 1 Select the plate that is located on the deck Press the Plate option in the SERIAL DILUTE menu PATE SEECTON Match the plate on the screen to the plate that is seated EINEN mmm on the VIAFILL plate deck After selecting the correct plate press the SAVE button See section 3 2 2 to see what tubes or which pipet tips to disconnect to dispense to a 6 12 24 or 48 well plates 384 Wel 534 Weil 2 Select the source container Click on a SOURCE option in the SERIAL DILUTE menu SOURCE 97 Select a Reagent Reservoir or a Plate as source j m F Press SAVE to continue il Facas Rapana Picte SAVE 3 Select the tubing set Press the
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