HistoViewer's User Manual
Contents
1. Select a specimen from a slide box on the right side Click Vwr and DB Vwr open the loupe function of HistoViewer i e the region of the specimen that can be labeled and annotated The Loupe window is the upper right window above the thumbnail DB opens the data base function If you no longer need the loupe or data base function you can simply close them by clicking on Hide or Hide database This frees space on the screen It is recommended to use a B p AA RE hs CEN RR y low to medium magnifica Pcs fr Teed ma Bn oar Large specimen window iarsi tion for the large specimen 2 end SAG te E ete window on the left side e g fussy eeei ase Se hte AB rag fins Sey Sigs ey 2x 10x or 20x and a larger Masks Cai St Pact toh Uae pee SRA Dy Sirs Teh Ln ot oy in the upper right corner e g 20x 40x or 60x The yellow square in the large left specimen window can position the desired detail in the loupe window Note that it is the position of the loupe window that can be meet AS magnification for the loupe eaweseneys 2 Say Nee be moved by dragging to labeled and is stored in the data base Now you can write a short text in the small white window on the right side in order to annotate the position You can also put one or more arrows on structures to label or set a rectangle on a structure Arrows of constant length are drawn from the arrow head side When you press on2. in f V09 ement sideboxis auhisto see point 1 If you have not selected a specimen box please select one on the right side e g Aarhus University Histology by clicking on the name of the slide box the selected slide box will turn wheat colored and the status will change to e g Your current slidebox is Aarhus University Histology Click Next 5 Your stored positions will now be visible on the left side of the window in case q Losin OOG EET fa Log in and select slide box Select positions some recently made positions are i i missing click Click to refresh Step 2 Content basophil granulocyte Click to refresh neutrophil granulocyte Click on all positions you wish to share fesoa eosinophil granulocyte By doing SO the selected positions will alarm Click to remove from list of attachments basophil granulocyte appear on the right side of the window f y7re gt neutrophil granulocyte monocyte Selected positions can be removed from _ J eosinophil oranuiocyte the attachment list by clicking on the position name on the right side Next 10 Page Click Next Finally you can specify the email recipient if you wish to send your message to more than one email recipient separate the recipients email addresses by You can also add a subject and write an email text Send your message by clicking Send The selected positions will automatically be
3. the left mouse button and move away from the position where you started clicking the color of the arrows changes Rectangles are simply drawn by clicking and dragging until the desired size is reached 8 Page Click If you wish to remove the labels reticular fiber Lymph de none 6 To store the position annotation text and or labels arrows rectangles click Store detail in database The position including the annotation text and any labels can be recalled from any computer connected to the internet once you are logged in under your user name 9 Page Sharing of Annotations and Labels Via the module Share registered users can send their positions annotations and labels to other users e g peer students To this end follow the following steps 1 Log in HistoViewer user name and password can be received Home Click to log in upon registration see section B Username Username Password Press ESC to close Sign in 2 To send positions annotations and or labels to 7 My Specimens other users click on Share in the upper panel 3 Click Toggle Message Window on the right side of HistoViewer 4 An Email guide opens now This email guide checks first whether or not you are ae logged in and whether or not a specimen Log in and select slide box box IS selected Login amp select slide box You are logged in as user name If you are not logged in please log
4. HistoViewer s User Manual Start HistoVie wer L tu NNERSIY FAQ Register HistoViewer Virtual Microscopy for Cell Biology and Histology What is it HistoViewer is a computer microscope We develop it as an accompanying tool for our microscopy courses Features Quickly fly over the specimen High resolution images using oil immersion Open multiple interactive windows at a time Create your personal annotations Exchange and discuss annotations with other users Do I have to register The short answer is no However registering will give you access to your personal database where you can store your annotations and labels and have access to the features you discovered at a later time point from any computer Which browser do I need Table of Contents General Operation of HistoViewel ess E EE NET EE asada erie E S EE O same duseesieemoe ee Registration eoceeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeEeeeEeEeeEeEeeEEeEeeEEeBeeeEEeEEEe EEE Ee l innotation and Labelin of Slides M E A PA EE SAE A AE AAEE O g e o Shat InN of l innotations and Labels CHOCHSHSHOOCHSHSEHOHSHHSHSHHEHOHSHSHOHHEHOHSHHSHHHOHOHHEHSCHHHSHHSHHOHHOHSHOHOHOHHOHOHOHOHOHOHOHOOHHOOOHOHOHOOHOOOHSHOHSCOCHOSCOHBOSCOHCOCHL EEOC HEOECOEE 10 g Website Disclaimer Limitation of Liability and Copyright cece cceseccccesseccceesseccceessessseeseeseeees 12 1 Page G
5. attached as links The repicient s will receive the message on the specified email account Please note that the 1 Login Attachments Log in and select slide box Select positions Step 3 Content Recipient s email user au dk Subject Granulocytes Finalize Write message and send Add your text here Previous spam filter used must allow receiving emails from HistoViewer 11 Page Website Disclaimer Limitation of Liability and Copyright There is no warranty for this website to the extent permitted by applicable law We provide this service as is without warranty of any kind either expressed or implied including but not limited to the implied warranties of merchantability and fitness for a particular purpose The entire risk as to the quality and performance of the program is with you In no event unless required by applicable law or agreed to in writing will any copyright holder or any other party who modifies and or conveys the program as permitted above be liable to you for damages including any general special incidental or consequential damages arising out of the use or inability to use this website or connected services or programs including but not limited to loss of data or data being rendered inaccurate or losses sustained by you or third parties or a failure of the website to operate with any other websites web services or programs even if such holder or other party has been advised of the poss
6. difies and or conveys the program as permitted above be liable to you for damages including any general special incidental or consequential damages arising out of the use or inability to use this website or connected services or programs including but not limited to loss of data or data being rendered inaccurate or losses sustained by you or third parties or a failure of the website to operate with any other websites web services or programs even if such holder or other party has been advised of the possibility of such damages We cannot guarantee permanent access to our website neither at present time nor in future This website is not designed to and does not provide medical advice All content including text graphics images and information available on or through this website may be erroneous and is not intended to be a substitute for professional medical advice diagnosis or treatment On submitting this form you confirm that you have read understood and will respect these conditions Agree and Submit 7 Page Annotation and Labeling of Slides Via the module joa registered users can store positions annotations and labels e g for later repetition and or sharing with other users To this end follow the following steps 1 2 3 Log in HistoViewer user name and password can be received Click to log in upon registration see section B Username user name Password Press ESC to close Sign in
7. eneral Operation of HistoViewer HistoViewer http anat microscopy ana au dk is a virtual microscopy tool for cell biology histology and embryology courses The slides of the e learning platform can be viewed from without registration However registering will give you access to your personal database where you can store your annotations and labels and have access to the features you discovered at a later time point from any computer As a registered user you can also share your positions annotations and labels with other users Up to date versions of all major browsers e g Firefox Google Chrome Safari Opera Internet Explorer gt 9 will work fine In the recent months some of these browsers achieved great speed improvements so it can pay off to test different ones Older versions of Internet Explorer from 8 downwards are not supported To get started follow the following steps 1 Go to the HistoViewer s website http anat microscopy ana au dk 2 Click Start HistoViewer in the upper right corner Start HistoVie wer MS NERS FAQ Register HistoViewer Virtual Microscopy for Cell Biology and Histology vy Hre PENS f my 3 A new site opens where you can see five tabulators are visible Home guides you back to HistoViewer s start page Click to log in guides you to the log in window My specimens guides you to the positions annotations and labels you have created only active whe
8. ibility of such damages We cannot guarantee permanent access to our website neither at present time nor in future This website is not designed to and does not provide medical advice All content including text graphics images and information available on or through this website may be erroneous and is not intended to be a substitute for professional medical advice diagnosis or treatment On using HistoViewer you confirm that you have read understood and will respect these conditions Any links to third party websites are provided solely for the purpose of your convenience Links made to other sites are made at your own risk and we accept no liability for any linked sites A link to a third party website does not mean that we endorse or accept any responsibility for the content or the use of such website We have not tested any content software feature or links found on any linked third party website and do not accept responsibility and liability for the quality safety suitability or reliability of any of the third party websites or any of the material contained in these third party websites Take your own precautions to ensure that the website content is free of items of a destructive nature e g viruses worms Trojan horses We bear no responsibility for the accuracy legality or content of the external site or for that of subsequent links All content of HistoViewer including its design scripts and scanned slides is protected by c
9. iewer have access to additional features such as a personal database where you can store your annotations and labels review the stored position annotations and labels from any internet connected computer anywhere and anytime and share these with other users specified by you To register follow the following steps AARHUS UNIVERSITY 1 To register click on Register on f wF Register the homepage j 2 A registration form will open Please fill out this form and read the disclaimer at the bottom carefully By clicking on Agree and Submit you agree to these conditions Note that the password will be sent to the email address specified by you Please make sure that the spam filter of this email address allows receiving emails from the Histo Viewer web server Histo Viewer Registration Form Please Register Choose a Username Email Class No Time of enrollment Can you read these numbers Website Disclaimer and Limitation of Liability There is no warranty for this website to the extent permitted by applicable law We provide this service as is without warranty of any kind either expressed or implied including but not limited to the implied warranties of merchantability and fitness for a particular purpose The entire risk as to the quality and performance of the program is with you In no event unless required by applicable law or agreed to in writing will any copyright holder or any other party who mo
10. le barlliflLoupe only 12 You can also select between two movement modes fly and move 13 You can also select a full screen mode by clicking E This will maximize the specimen window You can also close the side bar by rs clicking on the X this will free even more space y Specimens Specimen bo By clicking again on HI you can leave the full screen mode War 0 14 Clicking on pe TET opens the 3 viewers CsE Moves DB Hide module Besides the Feel cnn JS large specimen win Loupe window dow on the left side a Loupe window is the upper right window the lower right win dow is a thumbnail of the entire scanned specimen The position in the respective other windows is indicated by yellow and blue blinking boxes Clicking closes the loupe and thumb nail windows Clicking allows you to toggle between different window modes with and without loupe window E As RE 5 Page 15 You can open several windows with full functionality by clicking on the desired specimens in the specimen selection bar on the right side in this example two windows Lymph node at 10x1 n 38 i g Ae P fz 16 You can hide show a selected specimen by clicking on CI to reopen click E Likewise you can close E clicking on B or you can close all other windows except for the selected by clicking on B 6 Page Registration Registered users of HistoV
11. n Orceime 2 Skin elastic fibers lens 10x air NA 0 4 stain Resorcin 3 Skin collagen fibers 3 Page 8 If you r computer Home Click to log in screen or web lymphnode lt Lymph node browser window is Fes PN ES y rather small the peagi specimen will open with only a limited number of buttons To get access to all i Bea buttons click the 3 BiA Ar Wsi a Aarhus University Histology a AU Immunohistochemistry i ME ey a AU blood smears aed 4 af 4 f 1 Lymph node lymph node lens 60x oil NA 1 35 stain Gomori silver Lymph node 2 Skin elastic fibers lens 10x air NA 0 4 stain Orceine 2 Skin elastic fibers lens 10x air 9 You will see now the full MDI functionalities of HistoViewer Ee ETS If you have a larger screen web browser window you will see this design immediately when you select a specimen i e you will not see step 8 Bi 4 Sunes 2 Ps oe 7 Es y f AAA pt oat fees 10 You can now either examine the specimen by moving it click on the specimen and move the cursor or you can make use of the different functionalities 11 The Colors module can be used to adapt the colors contrast brightness to your own computer screen Likewise you can invert the colors and you have the i uu possibility to see a scale bar Contrast Adjust colors Blue Binvertlsca
12. n you are logged in Specimen boxes guides to a selection of the specimen boxes available and Share which guides you to creating email messages to other users only active when you are logged in Click to log in My Specimens Specimen boxes 2 Page 4 To examine the specimens click on Specimen boxes 5 Click on the desired specimen box e g Aarhus I saeseerrsne University Histology 6 All available specimens are now visible with a short description and a thumbnail of the specimen 7 Select the specimen you wish to examine e g 1 Lymph node lens 60x oil NA 1 35 stain Gomori silver Note that some of the specimens were scanned at different magnifications The value lens in this case lens 60x oil determines the magnification used to scan the specimen i e it is the maximum magnification however lower magnifications can also be selected in this case 1x 2x 10x 20x 40x Thus check that you have selected the magnification you wish to examine the slide Use the scroll bar to find the specimen you wish to examine Select a slide box Aarhus University Cell Biology Aarhus University Histology AU Immunohistochemistry AU blood smears Select a slide box Aarhus University Cell Biology AU Immunohistochemistry AU blood smears 1 Lymph node lens 60x oil WA 1 35 stain Gomori silver 2 Skin elastic fibers lens 10x air NA 0 4 stai
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