USER MANUAL
Contents
1. KOJAIR BIOWIZARD Golden Microbiological Safety Cabinet class II EN 12469 certified by T V amp A USER MANUAL Serial number Date of delivery Customer Kojair Tech Oy Tel 358 3 471 7000 info kojair com Teollisuustie 3 Fax 358 3 471 7077 www kojair com FINLAND The definition of the Microbiological Safety Cabinet is as follows A ventilated enclosure intended to offer protection to the user and environment from the aerosols arising from the handling of potentially hazardous and hazardous micro organisms with discharge to the atmosphere being filtered You are owner of one of the most modern and silent Class Il Microbiological Safety Cabinets available The Golden Line series is microprocessor controlled and therewith widely customizable All the standard configurations of Golden Line series are type tested and approved by TUV Rheinland according to current EN standard 12469 2000 the standard for Biosafety cabinets ISO 9001 CERTIFIED ORGANISATION NOTICE Only use this product for its intended purpose rev 3 01 A N KOJAIR t FEATURES AND SAFETY en 4 1 1 Introduction to the CADINGT ccceeccceeccceeeeceeeece cece eeseeeseeeeseeesseesaeeesseeesaeeeeas 4 1 2 OMS TC TON ANC FANG ere r EE EEEE EE EEE E sees es oes 5 1 3 Positioning the cabinet and safety instructions cc cece eeceeeeeceeeeeeeeeeeeeeeeeeeees 5 Ze USING THE GABINE Tipteers camese nee tneinae s2. mode button reset the timing The display shows Deleted after every deleted timing and when all timings have been reseted the display shows Empty After that you can make the same with daily settings With SET button you can go back if do not want to delete timer settings SHOW UV LIGHT AGE The display shows how long time the UV light has been on RESET UV AGE Resets the hour counter for the UV light FAN RUNNING TIME This function shows the time the fan has been running SET SOCKET TIMER rev 3 01 A KOJAIR Set the alarm limits to the fan and UV light The limit can be selected between SET LIMIT VALUES 2000 10000h for fan and 1000 10000 for UV light FAN 1 1 SPEED Shows the rotation speed of the fan to normal operation speed or 12 speed The current speed which is between 0 100 for 1 1 speed and 0 50 for 1 2 speed is seen on the display FAN 1 2 SPEED NOTE The values can t be changed Set the clock to real time Change the value with buttons with SET button to the next field ok SET TIME HH MM YYYY MO DD WD HH hours MMs minutes YYYY year MO month DD day WD day of the week SET LANGUAGES Selects the language English Swedish German Finnish French Dutch 2 4 DAILY MAINTENANCE AND CLEANING NOTE Before maintenance and cleaning it is mandatory always to check what kind of substances have been handled in the cabinet If cytostatics or other hazardous substances
3. have been handled in the cabinet special care has to be taken The person who is cleaning and servicing the cabinet should use a mask protective gloves and an overall More detailed instructions will be issued by the person in charge of the laboratory Switch the main key switch on The switch is located on the right bottom edge of the front panel Move the front window to lowest position and remove the cover plates of the lights Pull the quick latch down and slightly open the front panel Lift the cover plate slightly and pull it out at the same time Then do the same to the other cover plate picture 5 In order to clean the back of the front window you have to lift it up carefully picture 6c After cleaning put the cover plates back to their places rev 3 01 KOJAIR Front panel Quick latch Fluorescent tube Cover plate of the lights Picture 5 Removal of light cover plates NOTE Do not clean front glass with detergents or desinfectances around glass plate see picture below gio NOT CLEAN FRONT GLASS FROM THIS AREA cm AROUND GLASS PLATE WITH DETERGENTS AND DESINFECTANCES THIS AREA MAY ONLY BE CLEANED WITH WIFING CLOTH AND WATER r 13 A KOJAIR rev 3 01 NOTE Handle the front window with extra care See pictures 6a 6b and 6c Picture 6a Cleaning Picture 6b Cleaning Picture 6c Cleaning the bottom the bottom of the from inside of the sink worktop window Clean th
4. abinet Press MODE button to enter menu At first there is a selection user service with buttons Service level requires password Only Kojair Tech Oy authorized service technician is allowed to access service level password protected j SERVICE LEVEL PASSWORD Set values J MAINTENANCE Only in english Set downflow LOW Set exhaust LOW USER SETTINGS no password Lanquages Set downflow HIGH Set exhaust HIGH English Swedish Set fan 1 1 speed French Deutsch Dutch Finnish Set fan 1 2 speed SET LIGHT LEVEL Set button delay SET UV TIMER Set alarm delay SET SOCKET TIMER PEPPE tacky ov SET UV LIGHT AGE Save factory sets RESET UV COUNTER Reset fan hours FAN RUNNING TIME Reset hepa SET LIMIT VALUES Testing mode FAN 1 1 SPEED FAN 1 2 SPEED SET TIME Fan automatic tune SET LANGUAGE External fan mode Exit Fan stopping Remote control Hatch control Acoustic alarm mute 1 2 speed light Uv mode Data log 10 rev 3 01 A KOJAIR 2 3 1 USER SETTINGS Functions under the user settings are for daily use and using these functions doesn t harm protection level of the cabinet that s why you do not have to set any password to get access to them All the text in LCD display is shown with capital letters and texts can be shown with six different languages see table below Start the
5. cabinet by the main key switch The LCD display shows the current software version of controls and the cabinet also begins a self test MODE opens the menu and you can browse it by using and buttons SET allows you to select a function from the menu and or confirm selection Press button 8 MODE Display shows SERVICE USER Select USER level with By pressing and buttons the user can scroll through the functions A function is selected by pressing button SET button User setting functions are described in the table below USER SETTING FUNCTION DESCRIPTION Regulates the intensity in the working area between 60 and 100 By pressing the or buttons the display shows the value in 1 intervals To confirm the desired value press SET button The light intensity changes when the SET button is pressed Select first the function set or browse SET UV TIMER In set mode you can select daily or weekly mode In daily mode you select start and end times with buttons The function is selected by pressing SET In weekly mode you select first the day with buttons accept it with SET button and select SET LIGHT LEVEL finally the start and end times like in daily mode Exit with MODE button If you want to delete timer settings see below In read mode you can check or reset the timer settings The display shows first the weekly settings with next step down you can move in settings
6. e no external or conflicting airflow disturbances when it is operating such as open windows or personnel passing in front of it The cabinet should be switched on at least 15 minutes before starting the work so there is time to clean the workspace Before starting the actual work it is recommended that the work is carefully prepared so that equipment and material are moved in and out of the cabinet as little as possible A continuous in and out movement disturbs the airflow and weakens the protection given by the cabinet For the cabinet to give the best possible protection you should try to minimize rapid hand and arm movements as much as possible NOTE Beware that you do not obstruct the airflow by obstructing the grid on the worktable The safe working area is between the grids see picture 3 KOJAIR rev 3 01 Safe working area is between the perforated area and about 10cm from gas tap Safe working area is marked with blue retangle Picture 3 Safe working area While you are working the front glass of the cabinet should be in the working position If the front glass is not in the correct position the user will hear immediate warning from the alarm If you intend to switch off the cabinet when you have finished working it is better to close the front window before shutting down the cabinet However it is better if the cabinet can always be in the normal operating mode When you are not working in the cabinet cl
7. e workspace of the cabinet with mild detergents and desinfectance with a 70 alcohol solution You can wipe off the alcohol that has been sprayed on or you can simply let it evaporate The cabine s outer surfaces should be cleaned by wiping with neutral or mild base detergent solutions A NOTE The use of dissolvents for cleaning is not permitted Clean the work surface inside the cabinet The lower surface and collecting pan should always be wiped during cleaning Pictures 6a and 6b NOTE When cleaning the workspace of the cabinet you should be careful about the filter located above the workspace and also the sensor of the down flow indicator in the back wall of the workspace They can be easily damaged when wiping the cabinet and therefore should not be touched You can also use the UV light option for decontaminating the workspace it takes 30 to 60 minutes You can use the timer for timing the decontamination See chapter 6 1 Decontamination in service manual UV light must not be switched on when working in the same room UV radiation can harm eyes and skin of operators and cause damages to other materials A NOTE UV light is not recommended for decontamination by the EN 12469 Normally the cabinet requires only cleaning and changing the filters when necessary The filter change requires special knowledge and is done by service technicians trained by Kojair Tech Oy rev 3 01 A KOJAIR 3 WARRANTY The manufac
8. eni uous anceueswuneavaueansnubauewasswashnaneasnueneuneniays 7 2 1 Control FONTS steerer ercrcee aes areerige eteetereceeeckecmarnt hema wohe adie ghia wee wtheuatapetendaeestuesoaehese te 7 2 2 What tO do t TING SLO orseson nera Erer EEEE EEEE EEEE EEEE 9 2 3 Sone DANG TUMCH OAS e A N A E E 10 59 1 USerseWingS renser aiaia iiaa 11 2 4 Daily maintenance and cleaning cccsecccsececececeececeececeusecsusecsueessusessusesaaees 12 3 WARRANTY ssssansnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn nnmnnn ennan nnmnnn nnnm 15 xs KOJAIR rev 3 01 1 FEATURES AND SAFETY 1 1 INTRODUCTION TO THE CABINET Microbiological safety cabinets are intended to reduce the risk to the operator when handling hazardous or potentially hazardous micro organisms They do not necessarily protect the operator from all the hazards involved Some types of cabinet can also protect the materials being handled in them from the environmental contamination BioWizard Golden Line is a Class Il Microbiological Safety Cabinet operating on the recirculation principle It protects the products handled on the work surface from contamination and safeguards the operator against substances hazardous to health b A i y e Picture 1 BioWizard Golden Line Class II Safety Cabinet Examples of the uses of the cabinet are cellular culture handling pathogenic micro organisms isotopes sterile work for operations ha
9. er lead is in the electric plug on the top of the cabinet Turn the mains on by the key switch located on the right side of the front panel see picture 4 The self test can take 1 to 5 minutes and during the self test the cabinet does not take any orders During self test the red general alarm LED lights up other led s blink and cabinet gives an acoustic alarm acoustic alarm lasts only a few seconds Do not touch the controls during the self test Key switch Picture 4 Key switch The fan and the lights can be switched on by the pushbuttons located in the control panel Before switching the fan on you should check that the outlet on the roof of the cabinet is not blocked so that the filtered exhaust air can be freely exhausted out of the cabinet gt TO START UP CABINET PUSH BUTTON 3 Press for few seconds to start If sufficient air speed is not reached in 5 minutes the cabinet gives an acoustic alarm The key lock is activated by pressing SET button and START button in sequence When the key lock is activated the panel can be safely wiped etc without risking unwanted commands Green led s in the front window up and front window down buttons are blinking to indicate lock mode The setting can be deactivated by pressing the SET button and START button in sequence rev 3 01 KOJAIR 2 3 CONTROL PANEL FUNCTIONS The diagram below represents the hierarchy on menu options available in Biowizard Golden Line c
10. irculating air speed is not correct the general alarm LED Recirculating air alarm and the recirculating air alarm LED will light up and the acoustic alarm will sound The front glass of the cabinet should be in the working position during the work If the front window is not in the correct position The front window wrong position both the general alarm LED and the front window alarm LED will light up and the acoustic alarm will sound Alarm resetting button Not in use in this cabinet model rev 3 01 A KOJAIR Siart bution Press this button to start the cabinet Press for few seconds to Start Lights You can switch on workspace lights on using this button This button activates the socket You can move up the front window using this button Front window up a SSS se With this button you can browse through menus and set values Red led is used for indicating alarms When the fan has been switched on the general alarm LED lights up The general alarm LED lights stays on until the air speed is sufficient If sufficient air speed is not reached in 5 minutes the cabinet gives an acoustic alarm General alarm Working in the cabinet is not allowed while the visual and or acoustic alarm is activated You can switch cabinet to half soeed mode using this button The workspace lighting will shut down and the cabinet will give an acoustic and visual alarm The acoustic alarm signal lasts for only a few second
11. ndling medical substances in hospitals and pharmacies as well as the production and maintenance of electronic items in which particle free air is a necessity NOTE Only Class Ill Microbiological Safety Cabinets can be used for working with Group 4 pathogens The Kojair Biowizard BW3 is a class Ill safety cabinet A which protects the user and the environment from Group 4 pathogens Please contact your local Kojair representative for more information 1 2 1 3 rev 3 01 The basic principle of the cabinet is that the replacement air moves through the grid at the front edge of the work surface to the bottom basin and then again to the recirculation system Simultaneously it forms an air curtain in the work opening which prevents air from flowing outwards The recirculating air inside the cabinet is purified as it flows through the HEPA filter and it flows laminarly through the workspace see picture 2 t Plenum positive pressure marked with Negative pressure tt markedwith s a al Air curtain i E q Picture 2 Principle of the airflow CONSTRUCTION MATERIALS The main construction material of the cabinet is painted mild steel The work surface and the collecting pan located below it are made of stainless steel AISI 304 The front and side walls are made of 6 mm thick laminated safety glass POSITIONING THE CABINET AND SAFETY INSTRUCTIONS The cabinet should be positioned so that there ar
12. ose the front glass and device automatically switches to half soeed mode See chapter 2 1 Control panel It is strongly recommended that gas flames are used on the left side of the work space as short time as possible That is because the flame can disturb the airflow and it will warm up the recirculating air which can cause an alarm workspace is intact i NOTE Before starting work check that HEPA filter on the roof of the rev 3 01 p lt 7 KOJAIR 2 USING THE CABINET Zs CONTROL PANEL 3 4 5 6 8 SIOWIZARD amp 5 1 1 tn ha 2 o 5 oce nr x 8 of 5 ch Corson co CONTOL PANEL CONTOL BUTTON ALARM LED FUNCTIONAL DESCRIPTION DESCRIPTION Red led is used for indicating alarms When the fan has been switched on the general alarm LED lights up The general alarm LED lights stays on until the air speed is sufficient If sufficient air speed is not reached in 5 minutes the cabinet gives an acoustic alarm Alarm indicators Working in the cabinet is not allowed while the visual and or acoustic alarm is activated If the power failure occurs the general alarm LED lights up and the acoustic alarm will sound Working in the cabinet is not allowed during power failure KOUAIR se Ta If the exhaust air speed is not correct the general alarm LED and the exhaust air alarm LED will light up and the acoustic alarm will sound Exhaust air alarm If the rec
13. s Start switch cabinet to half speed mode The UV light socket is in the workspace back wall if you wish to use the UV light push the UV light button on the control panel There are two modes for UV light button or timer If button is selected the UV light will stay on until UV button is Uv light option pressed again If timer is selected the cabinet asks for shutdown time which is selectable between 20 120 min with 10 min interval Time can be changed with and buttons and time is selected with SET button UV light is switched off after selected time NO Used with optional gas valve If you use liquid gas during the procedure first you have to push the magnetic valve button from the control panel This enables the gas to flow The gas valve works only when the cabinet is at half soeed mode Front window down You can move down the front window using this button With this button you can confirm selection a With this button you can browse through menus and set values ee _h o_o o_o hk h O Magnetic valve _ x KOJAIR 2 2 rev 3 01 WHAT TO DO AT FIRST START UP NOTE Before using the cabinet perform the installation tests listed in chapter Testing in service manual and clean the cabinet carefully See the cleaning instructions in section 2 4 Daily maintenance and cleaning Check if the cabinet s power cable is in the socket outlet 230 VAC 50 Hz and the oth
14. turer of this product provides a guarantee under the following terms e The duration of this guarantee is one 1 year from delivery or from approved handover when no other delivery term has been agreed e The guarantee covers manufacturing raw material and material defects The customer is to inform the manufacturer of any defects without delay e The guarantee does not cover wear and tear components such as filters or fluorescent light tubes e The guarantee is not valid any more if this device is used for any other use other than its designed use or if any harmful substance is used which damages the cabinet material e The guarantee is not valid any more if any other spare part is used in replacement of original components as listed in the spare parts list In connection with the guarantee the manufacturer reserves the right to obtain any damaged component If such damaged component is not returned to the manufacturer during the duration of the guarantee then the manufacturer reserves the right to invoice for replacement components e This guarantee includes repair work carried out by the manufacturer as well as required replacement parts under guarantee e The guarantee is not valid any more if this cabinet is repaired or serviced during the valid guarantee period by persons other than personnel from the manufacturer or persons authorized by e the manufacturer
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