GXCapture 7 User Guide
Contents
1. Diagram 1 GXCapture s Main Window 1 Menu bar this duplicates the functions on the toolbar 2 Toolbar this duplicates the functions on the menu bar the shortcut buttons will open the corresponding menu function 3 View window for image editing 4 Video camera icon double click on the Video icon will open the live preview window to pop UP 5 Folder bar on left side this allows you to select a folder Any images in this folder will be previewed as thumbnails They can be opened into to GXCapture for processing o a O Gd hymenoptera solenopsis invicta j woolley texas a amp m ipg DDe esecese e 7 2 2 CIEUMEDELATROET ETD Operation Manual GXCapture 5 GXCapture s Live Image Interface A double click on the video switch button will trigger GXC s digital live image capture window which is shown as follows you can use the shortcut button F11 to expose or hide the toolbar Diagram 2 GXCapture s Digital live Image capture window 2 2 GXCapture s Image Processing Interface In GXC s main window click on the button Open of the toolbar or open in the dropdown list in File of the menu bar to import an image from a specified location Then select the required function from the toolbox Standard Measure Image and Status Bar from the dropdown list of the button View in the menu bar pteridiua3apcool10x2. 20485 1536 10244 765_ BIH S124e384 BIW SOO E00 ROL Diagram 8 Camera Resolution Setting Operation Manual GXCapture 11 Setting of Photographed Images When it comes to the settings of photographed images click on the settings button a first then continue to adjust the settings with the two tabs Image and Parameters in the dialogue box Digital Camera Setting You can save the parameter settings so that they are automatically loaded when the software is used next time to do this check the tick box at the bottom of the Parameter Tab Light frequency is used to reduce the beating effect sometimes encountered on microscopes and with room illumination you can select a frequency that does not coincide with the frequency of the illumination adjusting the exposure time can also eliminate this problem Digital Camera Setting Digital Camera Setting Contrast Frame Speed Saturation C Normal Speed High Speed ve Color Enhancement Light Frequency C50 Hz 60 Hz Image Quality O smooth Sharp Farameter Mode Oh Os Oc Load this mode when next start Diagram 9 GXCapture s Photographed Images Diagram 10 GXCapture s Parameters Settings Settings Interface Interface for Photographed Images Operation Manual GXCapture 12 3 2 GXCapture s Image Processing Functions This function applies to the processing of acquired images including cropping lightness and colour ad
3. q oe 2 gm K 3 t ATA Ye ml Vr aaa ONS pary P 4 Wate H 9 sage 4 p x 128 y 264 idx O RGBA 92 44 38 0 Time s 0 147 JPG 2048x1536x24 nm Diagram 24 Post Stripping Image 3 2 2 3 Colour of Image This function is used to adjust the colour of images including grayscale and dither JAAN Grayscale Function It applies to giving grayscale treatment to the image being opened Operating Instruction Use the following the sequence of Image gt Gray Scale then GXC will automatically initiate the grayscale processing and the result is shown as follows x 325 y 193 idx 204 RGBA 204 204 204 0 Time 5 0 020 IPG 2048x1536x8 NM Diagram 25 Image Given with Grayscale Treatment Operation Manual GXCapture 20 3 2 2 3 2 Dither Function It s used to give dither treatment to the image being opened Operating Instruction 1 Use the following the sequence of Image Dither then GXC will automatically cause the dither setting window to pop up as shown below Dither Floyd Steinber O Ordered dithe Burkes O Stucki O Jarvis Judice Hir O Sierra Stevenson Arce O Bayer matrix 4x4 ordered Diagram 26 Dither Effect Setting Window 2 In the above window after selecting the desired dither treatment effect click the button OK to return to the image processing interface GXC will then automatically initiate the dither tr
4. designed to provide a practical interface between the camera and GXCapture using this software you can achieve with ease the capture and processing of images and image sequences using live camera controls and preview facilities The following types of images can be captured Single Image Acquisition Series of images eg time lapse time separated Continuous video sequence with compression Single channel capture for subsequent merging for fluorescence imaging Low light image capture long integration periods Image Processing and Labeling The images acquired will be disolayed on the screen immediately and can then be processed with various tools for example Contrast lightness and colour adjustment Resizing and rotation of images Image filters such as sharpening White balance image merging and greyscaling GXCapture can be used to annotate the images with text shapes and calibrated scalebars The other major functional module of GXCapture is image analysis The values measured such as the length area and angle will be shown in a worksheet which can be exported for use in other programs such as Excel There is also a detailed wizard for the calibration procedure to ensure user friendly operation Operation Manual GXCapture 4 2 Basic Operation of GXCapture 2 1 Introduction to GXCapture s Main Page U GxCapture GxCapturet max i File Edit View Image Fiters Measure Help
5. environment of GXC Operating Instruction 1 Use the following sequence of Filters Mix GXC will cause the image mix setting window to pop Up as shown below Operation Manual GXCapture 32 OpType p Source options i T Mix alpha channel Diagram 43 Image Mix Setting Window 2 Designate the target image and original image in the above setting window set the mix mode and the values of X and Y then click on the button Refresh Preview to preview the post mix effect of the image in the preview area on the left 3 When the setting is completed click on the button OK GXC will then automatically initiate the image mix 3 2 4 GXCapture s Measurement Function You can measure the currently selected non live and display the results in the Measurements Table the measurement covers the distance between points areas the angle formed by two intersecting lines and point counts Itis necessary to calibrate the image before measurements are made and then vital that the appropriate calibration is selected from the calibration table that corresponds to the magnification used when the image was captured if you have zoomed the image or captured at a resolution that is different from the image used to calibrate the original calibration values cannot be used View 20071109 160019 bmp gy i file Edit View Image Filters Window Device Camera measure Help 15660 0 OK Can
6. s center to produce a contract effect Expand Aperture Row It embosses forward the center of the image to expand the aperture row Spiral It causes the image to have a distortion effect that peaks at the image s center Operation Manual GXCapture 28 and decreases as it reaches the edge to spiral the image Tubbulate It deforms the image as it were in a tubbulate item Overlap It deforms the image as it were a pile of overlapping images S207 Pseudo Colours Function It applies the filter Pseudo Colours to the image Operating Instruction Use the following the sequence of Filfers Pseudo Colours then GXC will automatically initiate the relevant treatment on the current image 3 2 3 0 Split Function It splits the colour image on the basis of the different colours hues saturations Lums and XYZ spaces The split of RGB will be taken as an example here to explain this function RGB RGB colour mode is a colour standard prevailing in the industry and represents the channels of Red Green and Blue HSL HSL colour mode is a colour standard prevailing in the industry and represents Hue Saturation and Lum YUV YUV is a colour coding method adopted by European TV system these three characters Y U and V don t form an English combined word instead Y represents Lum UV represents colour difference YIQ YIQ colour space generally prevails in the TV system of North America Y represents the graysc
7. the boundary line prominent Emboss It generates embossing and indents to various extents by drawing the outline of an image and decreasing the colour values of the neighboring area 32 341 Blur Function It uses blur filter to blur the image Operating Instruction Use the following the sequence of Filters Linear Blur on the menu bar then GXC will automatically start to blur the current image 3 2 3 4 2 Soften Function It uses softening filter to soften the image Operating Instruction Use the following the sequence of Filfers Linear Soften on the menu bar then GXC will automatically start to soften the current image 3 2 3 4 3 Gaussian Blur Function It applies Gaussian blur filter to treat the image with Gaussian blur effect Operating Instruction Use the following the sequence of Filters Linear Gaussian 3 3 Gaussian 5 5 on the menu bar then GXC will automatically start to treat the current image with Gaussian blur effect 3 2 3 4 4 Sharpen Function It applies sharpening filter fo sharpen the image Operating Instruction Use the following the sequence of Filfers Linear Sharpen on the menu bar then GXC will automatically start to sharpen the current image Operation Manual GXCapture 27 3 2 3 4 5 Edge Function It applies edging filter to edge the image Operating Instruction Use the following the sequence of Filters gt Linear gt Edge on th
8. 0 degrees 3 2 2 2 Image Transparency This function is designed for image transparency after which the image is still ready for removal split stripping and other operations SLAAN Great from lightness Function Defines the image transparency colour range Operating Instruction 1 Follow the sequence of Image Aloha Channel Great from lightness then users may transparentize the image being shown in the image processing interface 2 If you need to adjust the image transparency manually use Image Alpha Channel Opacity then the image transparency may be set through the settings window Opacity as shown below Opacity Level 0 aa Diagram 21 Opacity Setting Window Note The opacity setting window also applies to those images that have been treated by the function Great from lightness for a modification on image transparency for the second time Operation Manual GXCapture 17 3 After being processed by the function create from lightness the image will become the one shown below ER x 1112 y 853 idx O RGBA 225 201 177 49 Time 5 0 146 JPG 2048x1536x24 mM Diagram 22 Image Transparentized by the Function create from lightness 3 2 2 2 2 Remove Function This function is for removing the transparency effect existing in the current image Premise The current image has been transparentized Operating Instruction Use the fol
9. 63 Jpg jLength 226 57 um Time s 0 122 IE 204821536x24 Diagram 5 Result Measured by Line SPERI Rectangle Function It s used to measure the height width area and perimeter of the drawn rectangular Operating Instruction 1 Click on the button H of the toolbar or click following the sequence of Measure gt Rectangle on the menu bar 2 Move the mouse onto the image press the mouse s left key and drag the mouse to a proper position then press the mouse s left key again to release the measurement tool Rectangle Operation Manual GXCapture 38 Note please refer to the instruction given above for the application of other measurement tools such as Circle Polygon Angle and Point 3 2 4 3 3 Select Function Selects and moves the calibration result disolayed on the current image and allows you to adjust the border thickness Operating Instruction 1 Click on the button of the toolbar 2 Click on a Measurement result annotation in the image or onto the border of drawing 3 When the mouse is on the border or the measurement result a press on the mouse left key will enable the movement of the measurement result fo any position of the image Note when youmove the border the content of the said measurement result will move with it but the lines will remain still when the content moves 4 Double clicking on the border of a drawn line or sha
10. C will then automatically start the adjustment with the result as shown below Operation Manual GXCapture 25 agg x iiie y 600 idx O RGBA 228 192 163 0 i 2048x1536x24 Diagram 37 Image with a Contrast of 50 6 Draw the icon D along the bar Gamma of the setting window to preview different image display effects acquired with different gamma values Light Contrast Gamsma Lighten iia Contrast cl Diagram 41 Gamma Setting Window 7 When the setting is finished click on the button OK GXC will then automatically start the image adjustment 3 2 3 4 Linear User may apply tools including Blur Soften Gaussian Blur Sharpen Edge and Emboss to the treatment of image Blur It smoothes the image by weakening the colour contrast between neighboring pixels with a slight effect so as to softly soften any obvious edge or prominent shape Operation Manual GXCapture 26 Soften It softens a smooth edge or an area excessively sharp or of an excessively high contrast through producing a blur effect thereon Gaussian Blur It generates an obliterate thick blur effect on the image by adjusting the colour values of pixels and controlling the blur extent according to the curves of Gaussian Algorithm Sharpen It sharpens the image through increasing the contrast between neighboring pixels Edge It underlines the edges of an image to make
11. HF Y X E l Green 5 UF IF 1 cellstruct jpz w cellstruct jpe Vereen w Color cana V igs F Fisk cellstruct jpg gt w Alpha channel source Diagram 44 Image Combination Window Operation Manual GXCapture 30 2 Pick the desired colour space for the images to be combined out pf the dropdown list of item Colour in the image combination window then choose the channel to be used for combination and decide whether to use Aloha channel 3 When the setting is finished click on the button OK then GXC will initiate the image combination 3 2 3 10 FFT Fast Fourier Transformation Function It can apply FFT to the part satisfying certain conditions in the current image Fourier Transformation It can represent a function that satisfies certain conditions with a trigonometric function sine and or cosine function or the linear combination of their integral Operating Instruction 1 Click following the sequence of Filters FFT then GXC will cause the FFT setting window to pop up as shown below Real Imaginar F Inverse F Compute magnitude e Force FFT Diagram 39 FFT Setting Window 2 Set the transformation parameters in the window above Real Select the image to be transformed in the dropdown option list Imaginar Select the image towards which the transformation is oriented in the dropdown option list Inverse Checking this option represents the approval to the inversing du
12. _13 jpg 1 200 x 270 y 148 idx O RGBA 220 208 168 0 Time s 0 175 2048x1536x24 Diagram 3 GXCapture s Image Processing Interface Operation Manual GXCapture 6 2 3 Reference Buttons and Menus Toolbar Button Corresponding Menu Function a File Open Opens an image file Open File Save As Saves an image with a new filename The file Save As format can be specified na Edit Undo Undo the previous image processing steps Undo 1 a Edit Redo Redo the previously Undone image processing Redo steps a Edit Move Moves the displayed position of the image Mowe S Fa View Zoom Out Unzooms the image Zoom Out ee Helo Help Shows the relevant content of Help text Help c i Measure Line Selects the required measuring tool to measure Line Rectangle Circle Rectangle the image currently being viewed requires the Polygon etc prior selection of the correct calibration for C e accurate dimensional measurements Polygon Angle Point Pa Edit Measure Table Displays all the measurement data relating to the Table current image Edit Lock Locks the current measurement mode a E Yd Edit Delete Deletes the currently selected measurement annotation Operation Manual GXCapture 7 3 GXCapture s Functions 3 1 GXCapture s Photography Function In the main window of GXCapture users can select the of the camera from which the picture is to be live dis
13. ale value of the image and Q respectively represent the colour and saturation XYZ XYZisa Split method that s based on the region growing approach of XYZ space CMYK CMYK is a colour standard adopted by the printing industry C M Y and K respectively represent Cyan Magenta Yellow and Black Operating Instruction 1 Use the following the sequence of Filters Split Split to RGB then GXC will spit the image into Red channel Green Channel and Blue channel according to different RGB colours in the current image the results will be displayed in the image processing interface users may click on the tabs to view the post split image 2 The result of split is shown below Operation Manual GXCapture 29 x 362 y 346 idx 144 RGBA 144 144 144 0 Time s 0 000 2048x1536x8 mm Diagram 38 Result of RGB Split SAT Combining Images a powerful function used a great deal in fluorescence microscopy where different images have been captured from different filter cubes these images can now be combined Function It combines different images photographed by a black and white lens with the R filter G filter and B filter to form colour images Premise Two or more images must be opened in the environment of GXC Operating Instruction 1 Use the following the sequence of Filters Combine then GXC will cause the image combination window to pop up as shown below Combine Red
14. ame EL Length 100 Pixels 140 Mealnit ga OF Diagram 47 Calibration Settings Window 5 Click on the button OK to exit from the calibration adding window and confirm the calibration results 3 2 4 2 Calibration Table Function 1 Allows you to view select and change the stored calibrations 2 allows you to add and delete of calibrations using the Calibrate Table Operating Instruction View Calibration Result 1 Use the following sequence of Measure Calibrate Table GXC will then cause the calibrate table window to pop up 2 Click on the name of a calibration under the item Name such eo in the left blank of the calibrate table this will make the corresponding result to appear on the right column as shown below Name Length TotalPixel Unit Unit Pixel 140 00 Nane L1 Length 100 Pixels 140 MeaUnit um v add delete Apply To Image Cancel ve Diagram 7 Operation of Calibration Table Operation Manual GXCapture 35 Changing Calibration Values 1 Click on the calibration to be changed in the calibration window 2 Adjust the data including Name Length Pixels MeaUnit 3 When the editing is finished click on the button save to save the changes Add Calibration Result 1 Click on the button add in the calibration table window then add a new calibration result titled UnNamed as shown below Na
15. area measuring unit pixel Measure Circle To measure the radius of the designated circular area as well as the pixels and perimeter of such area measuring unit pixel cL Measure Polygon To measure the pixels and perimeter of the designated polygonal area measuring unit pixel Measure Angle To calculate the angle formed by two intersecting lines in the current image Measure Point To mark points in the image for measurement A Measure Remark To add remarks in the image Edit Backward To revoke the results of the last 20 measurement operations displayed on the current image ao o Edit Forward To restore the results of the last 20 measurement operations displayed on the current image 3 2 4 3 1 Line Function It s used to calculate the distance between two points in the current image and when the relevant Operation Manual GXCapture oT calibration needs to be selected for calibrated measurements to be seen Operating Instruction 1 Click on the button N of the toolbar or click following the sequence of Measure Line on the menu bar 2 Move the mouse onto the image press the mouse s left key and drag the mouse to a start position then press the mouse s left key again to release the measurement tool Line 3 As shown below the length of cells indicated in the image is the result measured by the measurement tool Line 1SView reamaysJmepcoolbz
16. background of the remarks transparent or not L Transparent Di Remark Please input remark Text Diagram 58 Post editing Remarks 4 Click on the button OK to add the post editing remarks to the image The follow image demonstrates the status where a remarks is successfully added to the image P 15View zeamays3upcoolSs 63 jpg height 161 43 um idth 2716 57 um Area 35283 70 Squm Perimeter 760 00 umj Diagram 59 Remarks Effect Setting Window Operation Manual GXCapture 41 3 2 4 4 Measure Table Function It s for viewing the information in connection with measurement and remarks already existing in the current image Operating Instruction 1 Click following the sequence of Measure Measure Table on the menu bar then GXC will cause the measurement data window to pop up as shown below Heasurelable Length Width Height Area Perimeter Radius Angle 193 33 136 19 26330 18 659 05 Diagram 11 Measurement Data Window 2 The content of measurement and the information of remarks already existing in the current image will be displayed in the window above OUTFUT 3 If there s a need to save the measurement data click on the button PARDEE in the lower left corner GXC will then automatically save the content of the Measure Table into the file titled Measurelnfo txt under GXC
17. cel i ax n G s Ogy Fiw x 926 y 0 idx 0 RGBA 56 56 85 0 Time 5 0 014 BMP 1280x1024x24 NUM a C My Docu E WorldPay s He NetObjects E 2 Microsoft git Sage 50 Acc w Microsoft v W o 10 03 Operation Manual GXCapture 33 3 2 4 1 Calibration Function Calibration is used to convert the width of one pixel into real units such as microns or millimeters This value is used in measurements and in the scalebar annotation Operating Instruction 1 Capture and store with logical file names eg 1OXPlanObj_0 5XCMount_1280x1024 jog a series images of a subject of known length eg a stage micrometer graticule each taken at different magnifications 2 Go to Measure Calibrate and the calibration screen will be shown as follows Calibration Wizard Tes for m Pixels 0 Diagram 45 Image Measurement Setting Window Load Image 3 Click on the icon to load the first of your images f the stage micrometer 4 Use the cursor on the image to mark a line between two points a known distance apart the wider apart the better For instance 100 microns apart Scale Info Diagram 46 Image Calibration Setting Window Operation Manual GXCapture 34 Enter the name of the calibration eg 10XPlan Enter the length of the linein real units eg 100 Select the measurement units eg um Scale Info N
18. e O22 lish Horizontal Mirror Function A click on the button Mirror will enable users to mirror the image being viewed Operating Instruction 1 The image shown below is the pre mirroring one Operation Manual GXCapture 14 P TSView pteridiua3apcool10x_13 jpg x 3T y ST4 idx O RGBA 129 139 89 0 Time s 0 145 JPG 2048x1536x24 Diagram 4 Pre Mirroring Image 2 Click on the button Ea on the right toolbar the image will be mirrored as shown below gt 2 xi 1150 y TT4 idx O RGBA 232 197 175 0 Time s 0 013 TPG 2048x1536x24 Diagram 5 Post Mirroring Image S222 12 Reversal After reversed the image titled Diagram 5 will become the one shown below Operation Manual GXCapture 15 x 3T y ST4 idx O RGBA 129 139 89 0 Time s 0 145 JG 2048x1536x24 NM Diagram 6 Post Reversal Image 3 4 2193 Rotation Function Click on the button Rotate L3 and set a certain rotation angle through the rotation setting window subsequently popping up to rotate the current image Operating Instruction gj 1 A click on the button Rotate 17 will command GXC to pop up the rotation setting window shown below Rotate Before Diagram 20 Image Rotation Angle Setting Window Operation Manual GXCapture 16 Angle degrees oo Rotation angle setting Left rotation for 90 degrees Right rotation for 9
19. e menu bar then GXC will automatically start to edge the current image 3 2 3 4 6 Emboss Function It uses embossment filter fo emboss the image Operating Instruction Use the following the sequence of Filters Linear Emboss then GXC will automatically start to emboss the current image 32 3 0 Non linear Users may apply filters including Noise Medium Value Erode Swell Contour line Edge and Undulate to the image Please refer to 3 2 3 8 Linear for the application of such non linear filters Noise The application of this tool will create some random interfering particles on the image namely the assorted colours Medium Value It can adjust and make medium the intensity of each pixel in the image Erode It can produce an erosive effect on the image s colours Swell It can swell the image Contour Line It can draw fine lines along the edges of different colours in the image and identify the contour lines of each colour channel Edge It can make prominent the edge of the image to underline the boundary line Undulate It can create undulation effect to the image 3 2 3 6 Deform filter User may apply filters including Contract Exoand Aperture Row Spiral Tubbulate and Overlap to deform the image Please refer to 3 2 3 8 Linear for the application of the aforesaid filters Contract It contracts the image around the image
20. eatment the image shown below is a result of selecting the option rdered dithe P ISYiew pteridium apcool1l0zx_13 jpg Hile Hit Wer Inage Filters Measure Help a View Op aaa Ro Flip Mirror Resan __ TView pteridiua3apcool 10x 13 g Ready 75 0 JPG 2048x1536x1 NM Diagram 27 Image Given with User defined Dither Treatment Operation Manual GXCapture 21 32 3 GXCapture s Filters Image adjustments may be realized by applying filters including the lightening and darkening of image the increase in contrast and the application of Linear filter Non linear filter and Deform filter Corresponding Menu Function Filters Repair To set the repair parameters to repair the image used to reduce scratches and noise in the image Filters gt Linear Sharpen To sharpen the image Filters gt Linear Blur To blur the image Filters Linear Light Contrast Gamma To allow users to manually adjust the lightness and darkness of the image Filters Linear Light Contrast Gamma To allow users to manually adjust the contrast of the image 32 3 ie Threshold Function To convert a gray or colour image into a high contrast black and white image Operating Instruction 1 Click following the sequence of Filfers gt Threshold then GXC will cause the threshold setting window to pop up as shown below Threshold Level 0 1a1 Diagram 32 Threshold Setting Window 2 Set the
21. eration Manual for GX Capture GXCapture 7 User Guide Version 7 Operation Manual for GX Capture Please note that due to our policy of continuous product enhancement parts of this manual may vary slightly from the delivered product The appendixes attached hereto include no guarantee of the technical processes or product characteristics described herein In addition GT Vision shall not be held liable for any printing error or inaccuracy contained herein except to the extent that GT Vision is aware of the existence of the same or the same is caused by GT Vision s intentional neglect This Guide only gives a general picture on the relevant information and technical processes and doesn t apply to all kinds of circumstances If there s any doubt please consult GT Vision This Guide is under copyright protection GT Vision reserves all the rights to this Guide The content hereof will be Updated according to the technical modifications and supplements of the products on a regular basis This Guide only reflects the current conditions of GT Vision s software Copyright 2010 by GT Vision Operation Manual GXCapture 3 1 Introduction to GXCapture GXCapture is specialist modular software for image acquisition processing and analysis from GXCAM cameras hereinafter referred to as GXC If comprises of two main modules Image Acquisition GXCapture can be used with GXCAM cameras and the cameras supplied by GT Vision It is
22. ime stamped Time Lapse Diagram 6 Photography Setting 2 Name the image to be saved named with the current time of the system checking this option will select this file naming method if not users will be required to manually enter the name of the image to be saved in the blank dialogue box on the right of the file name hl B Use bmp to set the extension and format of the image to be saved such as Imagel omp Image 1 jog options are available in this dropdown list Once captured the image can be resaved into a much wider variety of formats in the image processing File sSave As menu For timelapse simply enter the interval in seconds between each capture and the total number of images required OF 3 When the set up is finished click the button to save the settings Operation Manual GXCapture 9 3 1 3 Video Sequence Capture Click following the sequence of Snap on the toolbar gt menu Config gt dialogue box Config Property of Save and then designate the save path through tab Video Contig Froperty of Sare Use File Save Dialog Use File Save Contig Video Compressor vid WPEG 4 Codec A click on the button OK will turn sequence record to hard disk function or stop the videotaping if the video sequence record function is into a click on this will then start the video already in progres
23. justment of acquired images 3 2 Ve GXCapture s Tools GXC s tools include Move and Zoom Toolbar Button Corresponding Menu Edit gt Move To move the image that has been zoomed in At a View Zoom In Out Zoom in or zoom out the image to be viewed Zoom In Zoom Out 3 2 1 1 Move Tool If the image is beyond the display area of GXC after zoomed or for its original size user may use the move tool Mowe in the dropdown menu under button Edit of the toolbar or menu bar to move the image in four directions to take a full view of it Clee Zoom Tools Function Zooms the image to be viewed Operating Instruction D Zoom In Zoom Out 1 Click on the buttons to select the zoom tool 2 Move the mouse on the image and press the mouse left key to zoom the image being viewed Operation Manual GXCapture 13 3 2 2 GXCapture s Image Processing This function is for the processing of acquired image including adjusting the reversal degree inclination degree and colour of the image 32 2 1 Reversal of Image The reversal of image includes mirror reversal left reversal right reversal rotation and inclination Button on the Right Corresponding Menu Toolbar Image Processing Horizontal To mirror the image being viewed Mirror Image Processing Reversal To horizontally reverse the image for 180 degrees Image Process Rotation To rotate the image for a certain angl
24. lowing the sequence of Image Alpha Channel gt Remove to remove the transparency effect existing on the current image and restore it to its original state D222 Split Function To create a new black and white image out of the current image which has been transparentized Premise The current image has been transparentized Operating Instruction 1 Use the following the sequence of Image Alpha Channel Great from lightness to Operation Manual GXCapture 18 transparentize the current image 2 Use the following the sequence of Image Aloha Channel Split and then GXC will automatically generate a new black and white image which is shown below P TS View Alpha Channel 3 from pteridium3apcool10x_13 jpg a7 s y 7 i a fd Filt e x ay 385 idx 37 RGBA 37 37 37 0 Time s 0 000 2048x1536x8 Diagram 23 Image Generated by Split 32224 Strip Function This function can strip the image that has been transparentized and then generate a new stripped image Premise The current image has been transparentized Operating Instruction 1 Use the following the sequence of Image Alpha Channel Great from lightness to transparentize the current image 2 Use the following the sequence of Image Alpha Channel gt Strip then GXC will automatically generate a new stripped image which is shown below Operation Manual GXCapture 19 S A te r
25. lue Operation Manual GXCapture 23 3 When the setting is finished click on the button OK and then GXC will automatically initiate the image adjustment FA User defined Lightening Contrast Gamma Function Users may define the lightness darkness contrast gamma correction value and other parameters of the current image at will Operating Instruction 1 Use the following the sequence of Filter Light Contrast Gamma GXC will then cause the lightness setting window to appear as shown below Light Contrast Gamma Lighten SEEE Contrast a j Gamma a Diagram 34 Lightness Setting Window 2 Set the lightness value in the lightness setting window the default lightness value of GXC is 0 which is increased to 50 now for a more obvious lightening effect 3 Click on the button OK to return to the image processing interface GXC will then automatically start the image lightening whose result is shown below Operation Manual GXCapture 24 a ae a ae ae 10 T iewy pteridiua3apcool10z_13 jpg e 20 w x 1048 y 852 idx O RGBA 147 130 145 0 Diagram 35 Image with a Lightness of 50 4 Set the contrast value at 50 in the contrast setting window GXC s default contrast value is 0 Light Contrast Gamma Before Diagram 36 Contrast Setting Window 5 Click on the button OK to return to the image processing interface GX
26. me Length TotalPixel Unit Unit Pixel LI 100 00 140 00 um 0 71 Nane UnNaned Unllaned Length 1 Pixels l HealUnit um add delete Apply To Image Cancel Diagram 8 Additional Calibration Result 2 Click on the additional calibration result Under the item then rename it as L2 and change its Pixels to 101 in the right column after save the changes Length TotalPixel Unit Dnit Pixel i 100 O0 140 00 um 0 TI Name Le 101 00 101 00 1 O0 Length 101 Pixels 101 Mealnit um add delete l Apply To Image EAE Diagram 9 Additional Calibration Result after Varied Delete Calibration Result 1 Click on the calibration result to be deleted in the calibration table window 2 Click on the button delete then the deleting of the designated result is finished Operation Manual GXCapture 36 3 2 4 3 Measurement Tool These tools are for measuring of the distance between two points the angle formed by two intersecting lines and the area of a shape drawn in the current image Toolbar Button Measure Select To select and move the calibration result displayed on the current image and to vary the border thickness of such result Measure Line To measure the distance between two random Corresponding Menu points in the current image Measure Rectangle To measure the height and width of the designated area as well as the pixels and perimeter of such
27. pe opens the properties Properties Name shape Color 4 Text Line Color QM BkcColor ABC Transparent Fils Default OF Cancel Diagram 54 Properties of the Result Measured by Rectangle 5 In the properties window you may change the text font and drawing appearance of the current selected drawing 6 Click on the button OK to return to the image processing interface Note the border thickness of all measurement tools shall fall between 1 and 4 without hitting the both ends Operation Manual GXCapture 39 R1 height 161 43 um ER 218 57 um jArea 35283 70 Squm gt Perimeter 760 00 um x TA y ToO ide O RGBA 207 192 233 0 o Time ls 0122 o Poo UAB SEK eA Diagram 56 Rectangle measurement after being changed in Properties 3 2 4 3 4 Remark Text annotation Function It s Used to add marks and remarks to the image for viewing image information Operating Instruction 1 Click on the button A of the toolbar or click Measure Remark on the menu bar 2 Move the mouse onto the position where the remarks is needed in the image press the mouse s left key this opens the editing window EKemark Please input remark Text Diagram 57 Remarks Editing Window 3 In the window above users can edit the text of the remark change the typeface through Operation Manual GXCapture 40 td BkColor change the colour through and make the
28. played captured and imported the basic settings of the camera at the time of display amp capture and the live image preview 3 1 1 Start the Camera Double click the Video icon in the viewing area The live preview window will then pop up If the camera is connected to the computer and an applicable drive is detected it will start displaying the live video automatically PE Col Diagram 4 GXCapture s Live Preview Image If the camera is not connected to the computer or no drive is found al following window will pop UD GxCapture X A No Camera Diagram 5 GXCapture s No Camera Interface At this point please make sure whether the driver has been correctly installed or whether the camera is connected to the computer Operation Manual GXCapture 8 3 1 2 Camera s Live Image Display amp Capture Settings Single Image amp Timelapsed Image Sequence Capture Click on 8F_ to acquire images Image naming configuration You can set up how each snapped image is named by selecting the pulldown list of a the the dropdown window Config will pop up click on the item Config to open the dialogue box Config Property of Save and follow the set up procedures shown below 1 Click on the tab Snap the photography setting window shown below will appear Config Froperty of Sare Ose File Save Dialog Use File Save Contig w Use T
29. ring the transformation Compute magnitude Checking this option represents the approval to the calculating of absolute value during the transformation Force FFT Checking this option represents the approval to the occurrence of Force FFT during the transformation 3 When the setting is completed click on the button OK GXC will then automatically initiate the transformation 3 2 3 11 Repair Function Operation Manual GXCapture 31 It repairs the colour image according to the different colours hues saturation lums and XYZ spaces Operating Instruction 1 Use the following the sequence of Filters Repair GXC will cause the repair setting window to pop Up as shown below Color Radius Tteratic Diagram 40 Repair Setting Window 2 Set the colour radius iteration of the image after repaired Colour Select the colours to be changed to in the dropdown option list such as RGB or HSL Radius Set the radius to be changed to manually Iteration Set the iteration to be repaired to manually 3 When the setting is completed click on the button OK GXC will then automatically initiate the image repair 3 2 3 12 Image Mix Function It can mix multiple images into a new one Note Different from image combination image mix doesn t involve the image s colour soace and requires no setting of RGB HSL and other channels Premise Two or more images must be opened in the
30. s Re accessing the setting interface and clicking on the tab Snap will switch it back to the photography settings interface 3 1 4 Settings for Video Sequence Image Recording Exposure Time Setting F Click on the exposure button Hl on the toolbar of the video playing window or on the settings button a in the tab Main to set the exposure time in M Minutes S Seconds m milliseconds The longer the exposure the slower will be the live image refresh and the more light will be gathered by the camera before delivering an image to the display UO 00 43 Mi So om _ Auto E sposure 4 Operation Manual GXCapture 10 Automatic White Balance Click on the Auto WB button la in the video playing window to set the white balance This is best done when the camera is pointing at a background image with no specimen Make sure the image is not over saturated to white with too much light Area White Balance After the target area is selected click on the Area WB button W in the video playing window to set the white balance This is used when you want to select a background area in your image that should be white grey Camera Resolution Setting Select the desired video resolution via the dropdown window of the video playing window On some cameras this will also affect the resolution of the captured image even if the captured image resolution is set higher 045 1556 320 240
31. threshold at 118 in the dialogue box of threshold setting window 3 OK GXC will automatically starting the conversion of the current image according to the set threshold and the result is shown below All the pixels brighter than the threshold 118 are converted into white while those darker than the threshold are converted into black Operation Manual GXCapture 22 1sView pteridiua3apcooll0x_13 jpg File Edit View Image Filters Measure Help F ofa amp A B p fj Select Calibrate Calibrate Table Line Rectangle Circle Polygon Angle Point Remark Table Lock Delet a w uk PA LA E ga AVE TN gt 2048x1536x1 NM Diagram 33 Image Converted with the Threshold 118 e A Colourize Function It s mainly used to adjust the values in connection with the RGB colour and HSL space and to calculate the specific RGB value of an image Operating Instruction 1 Use the following the sequence of Filters Colourize then GXC will cause the colourization setting window to pop up as shown below Colorize HSL Hue 0 Saturation 0 Blend C RGB Red 255 Green 255 Blue 255 Diagram 42 Colourization Setting Window 2 Select HSL or RGB colour mode in the colourization setting window If HSL mode is chosen users may vary the hue saturation and blend if RGB mode is chosen users may vary the values of red green and b
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