Operators Manual Operators Manual for mikura Microplate Washer
Contents
1. IEN 0 cinen eser eree ae arei isee E EAEE NEERA 5 on and power supply is Operating IAS TUCU NS sar e e an daad aeda iaa a e S 8 plugged into system Replace Fuse Disp nse pressure adjustment seeni E AEE REE AA E 9 Fuse has blown Cleaning and disinfecting ccs ieisesiersestuacbuiens veasesieicnabuadsadeadsbloaiuateidiabesdalencatiedatieeacieedees 9 TOUDE SVEN NG a a eiaa aties ea a ett Palen aml EE a ek 10 System will not prime Liquid level in wash bottle is Fill wash bottle y P a Wanra eee eee Raton ee EEEIEE EEEE NEETI EA EEE E E E E E Carer 10 Return Tor repa suren ne E EEE EEE E T E ERR 11 Bottl is Tight DA reer ad E EE thane OEE EAEE a asiee onesie ca atian cn cc ou cera TE EAE A EE ATETA 11 Tubing incorrectly assembled Follow set up procedure Ordering ae et Ute a a a a a a a a 11 Dl Aloe S E E E N 11 Uneven dispense One or more tips are blocked Use small wire provided to with dried buffer solution clean tips Uneven aspirate One or more tips are blocked Use small wire provided to with dried buffer solution clean tips Liquid delivers too vigorously Pressure set too high Adjust pressure screw until and aspirates slowly desired delivery and aspirate rate is achieved Warranty All goods supplied are guaranteed for a period of 12 months from the date of despatch Any item found to be defective during this time through faulty materials or workmanship will be repaired or replaced No warranty claims can be considered if2. ding to the specified test methods Ordering Data For a list of Mikura distributors please contact Mikura Ltd Tel 44 0 1403 891875 Spinningwood Farm Fax 44 0 1403 892651 Burnthouse Lane email info mikura co uk Lower Beeding West Sussex RH13 6NN England Spare Parts Spare Wash Head 8 port manifold Cat No 200 008 Spare Wash Head 12 port manifold Cat No 200 012 Spare set of tubing Cat No 200 500 De clogging tool Cat No 200 2010 Pressure Release Filter Cat No 3503 Fuse 20mm long T1 6A Cat No 6015 European Power Supply continent 230V 50Hz Cat No 6001 Great Britain Power Supply 230V 50Hz Cat No 6000 USA Power Supply 120V 60Hz Cat No 6002 Australia Power Supply 240V 50Hz Cat No 6003 Complete packaging Cat No 7500 Pt No 200 6000 11 Issue6 mikura Microplate Washer Operators Manual mikura Microplate Washer Operators Manual Table Of Contents Trouble Shooting The Microplate Washer has been designed to be reliable and require very little maintenance however should problems occur the following chart will help diagnose and remedy the problem a SOU MED tg fee i a ig a8 PN a Ni BN a Na Dale la ty Dale aa Data ra Dd Page No DAC KINIC HAS EMU CUI OWS xt ceczetuce sat E AE E eben ua usde Aven O EO OEE E 4 Problem Likely Cause Remedy NASIOC Stent ttl bt ei sa leet cs 4 Power light will not illuminate No power to pump module Check main power is switched Connecting your Microplate VV AS
3. e just below the top of the well and press the dispense valve until all wells are full Any excess liquid will be aspirated off so the wells will not overflow Repeat until all the strips have been washed Pt No 200 6000 8 Issueo mikura Microplate Washer Operators Manual Connecting your Microplate Washer Connect all tubing following the colour coding which has been applied for your convenience to ensure correct connection Place the wash head on the support provided Plug in the power supply to the socket provided see fig 1 Your system is now ready for you to use Figure 1 lE Fuse G DC Socket Pt No 200 6000 5 Issueo mikura Microplate Washer Operators Manual mikura Microplate Washer Operators Manual Bottie Screw cap Pressure Release Filter Filter Holder bead Needle Valve we Bottle Cap Manifold i I L amp S amp Sa ob Dispense Valve Start Button AES Plate Holder Support L _ANXSI K a Plate Holder ea SSS Wash Bottle Waste Bottle hi m emp Wash Reagent Riser Tube Suction Tubing Dispense Tubing Wash Head Waste Riser Tube Dispense Aspirate Nozzies Pt No 200 6000 6 Issue6 Pt No 200 6000 7 Issueo
4. he needle valve on top of the wash bottle cap manifold Using a flat bladed screwdriver turn the needle valve CW in order to increase the pressure in the bottle and CCW in order to reduce it Replace the bottle top filter holder over the needle valve Cleaning and disinfecting Fill the wash bottle with reconstituted Virkon and while following the priming sequence flush the system through On completion flush through with diluted alcohol and then distilled water to remove all traces of de contaminant Sterilisation should be carried out to fluid path components only Chemical sterilisation is permissible by soaking fluid path components overnight in a dilute 1 1000 solution of Sodium Hypochloride Components must be rinsed well afterwards in sterile distilled water in order to clean off any residue of the sterilisation media Steam sterilisation of fluid path components is permissible at 134 C 2bar after the unit has been cleaned using the standard cleaning procedure above The pump module and the plate holder are not autoclavable and must therefore be cleaned using a hard surface de contaminant such as Virkon Important read and follow the instructions enclosed with de contaminant carefully Pt No 200 6000 9 Issueo mikura Microplate Washer Operators Manual Operating Instructions Before operating the Microplate Washer ensure that the waste bottle is empty and the wash bottle has enough wash buffer to co
5. limits of its chemical compatibility and mechanical properties listed below OoN D DJIAUN Wash Head PTFE Dispense Valve PTFE Dispense Valve O ring Silicone Dispense Nozzles PEEK Dispense Tubing Tygon Suction Tubing Tygon Bottle Cap Manifold Polypropylene Bottle Screw Cap Polypropylene Wash Reagent Riser Tube PTFE Waste Riser Tube PTFE Wash Bottle amp Waste Bottle Polycarbonate Plate Holder Polyurethane Pt No 200 6000 4 Issue6 mikura Microplate Washer Operators Manual Important always follow the wash protocol as directed by the assay supplier When the protocol has been completed return the wash head to the support Important If the system is left idle for more than 30 minutes dispense some wash reagent into the prime trough until it is full Turn off unit and wait 5 seconds until vacuum has stopped and then place wash head on its support The tips will now be immersed and will not dry out If the system is left idle for more than 8 hours fill the prime trough with distilled water and place the wash head on the support so that the tips are immersed in the liquid If the system is left idle for more than 48 hours empty all reservoirs and purge all lines with air Dispense pressure adjustment The pressure in the wash bottle is pre set by the manufacturer However should you need to alter the it from the pre set value follow the following steps remove the bottle top filter with its filter holder which covers t
6. mikura Microplate Washer Operators Manual Operators Manual for mikura Microplate Washer WEEE COMPLIANCE This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol z Mikura Ltd has contracted with one or more recycling disposal companies and this product should be disposed of or recycled through them Further information on Mikura Ltd compliance with these Directives the recyclers and information on Mikura Ltd products which may assist the detection of substances subject to the RoHS Directive are available on request It is the stated philosophy of Mikura Ltd to preserve the environment wherever possible Mikura will only use materials and production techniques that cause least environmental damage for this reason Mikura does not use PVC in its products Pt No 200 6000 12 Issue6 mikura Microplate Washer Pt No 200 6000 Operators Manual Issue6 mikura Microplate Washer Operators Manual Return for repair For safety reasons only clean de contaminated instruments can be checked or repaired Always clean and de contaminate the equipment before sending it back Complete a declaration on Absence of Health Hazards State the nature of the problem and the liquids used with the unit CE The CE sign certifies that the instrument meets the requirement of the EEC directive and has been tested accor
7. mplete the protocol you are about to carry out To empty waste bottle unscrew bottle cap and remove cap manifold pour waste into container that can be used for secure disposal See cleaning and disinfecting page 7 Important always empty the waste bottle before use Do not allow contaminated liquid to enter the pump To fill wash bottle unscrew bottle cap and remove cap manifold fill bottle with buffer replace cap assembly and tighten cap Important never fill above the 2 litre line on bottle Place wash head on the plate holder support the tips will now be inside the prime trough Apply power and press the start button at this stage the pump will start and a low purring noise will be heard Allow 10 seconds for the pressures to stabilise With the head in its rest position on the plate holder support press the valve button on the wash head for 5 seconds or until all the air bubbles in the feed line have been removed While the head is dispensing the aspirate tips will be removing any excess liquid from the prime trough This should be repeated until you are satisfied that the system is fully primed Place the microplate on the plate holder ensuring it is located evenly and is orientated to correspond with the wash head Hold the wash head at each end and lift it from the support move it to the first strip to be washed and lower the tips into the wells this will remove any excess liquid that may be there Raise the tips so they ar
8. repairs other than those stated above have been made or attempted Pt No 200 6000 10 Issueo Pt No 200 6000 3 Issueo mikura Microplate Washer Operators Manual Unpacking Instructions Read this manual in its entirety before proceeding On receipt of your new instrument check that the outside of the package is intact and does not show any sign of external damage If any damage is seen note the particulars before proceeding Important if on receipt the product is found to be damaged report this to your distributor within 48 hours so further action can be taken Carefully slit the tape and open the outer carton lift off the two polystyrene covers and expose the components Carefully remove them from the packaging and their protective wrappers and place them on a secure and level bench The package will include 1 x wash head 1x pump module 1 x plate wash head holder 1 x 2 litre wash bottle assembly 1 x 2 litre waste bottle assembly 1 x DC power supply 1 x accessory pack 1 x operators manual 1 x De clogging tool Please contact your distributor immediately if the contents vary from this list Important Please retain the packaging for future use Important Use the pump module only with the power supply provided Before use please check that the power rating on the supplied unit matches the available mains supply Materials Before starting to work with your Microplate Washer check that the reagents you intend to use are within the recommended
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