Sutter Operating Instructions
Contents
1. loop as looping reduces the amount of control you have over your pipette diameter Low Pull values of 40 75 produce better injection needles whereas values between 120 250 produces good micropipettes Time values between 150 200 give the pipette a taper depending on the length of time Sets cooling time during slow pull 8 Place your glass pipette into the grove on one of the clamps blue arrow Fig 1 9 Carefully pull your pipette clamps together using the bars red arrows Fig 1 being careful that the pipette goes through the centre of the filament without damaging it If you don t pull clamps together the puller will not heat 4 hj r Bh Ng N a A 3 Fig 1 10 Keeping the pipette clamps together with one hand tighten the clamp screws green arrow Fig 1 to hold your pipette in place 11 Press Pull on the keypad green button2. Sutter Operating Instructions 1 Turn On Lower Left Hand Side 2 You will then be asked which pre saved program you would like as shown below WHICH PROGRAM DO YOU WANT 0 99 COPYRIGHT SUTTER INSTRUMENT CORP 1992 3 Typically select program 1 unless you have saved a program RAMP TEST for new glass 1 If you have a new box of glass pipettes you will need to perform a RAMP TEST to ascertain the melting point of this glass 11 Press CLR on the keypad 11i Press O to not clear parameters iv Select 1 for ramp test v Install glass and press pull vi The machine will pull the glass and give a figure for the heat required to melt your glass 4 If you wish to adjust the program settings simply type over them pressing ENT moves onto the next value pressing NEXT moves to the next line 5 Pressing CLR on the keypad gives access to air pressure settings this is used during the cooling phase of the pull thick walled glass value 500 thin walled value 300 6 You will be asked if want to destroy the program select No Unless you really do want to wipe the program 7 Adjust the Heat Pull Velocity and Time settings Here is a sample setting which will pull a pipette using the GC150 pipettes provided o se pA Increasing the heat for the last pull will widen the end of the pipette whereas a high heat will result in a very fine pipette tip Its best to have settings so that it pulls on the last line and doesn t go into a
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