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PAL User Manual Installation and Operation HTS

1. No Processing Step Method Object Class Used Object Parameter Value Reference X Y Z axes and syringe System Start Ref ON plunger 2 Initialize injection valve Inject to Injectors If SplTrnsfDel 0 SplTrnsfDel then switch to STANDBY INJECT else switch to ACTIVE LOAD 5 Initialize cut valve Cut Valve Injectors If Cut Time gt 0 Cut Time then switch to STANDBY 4 Start timer MinCondTime MinCondTime 5 Wait for Start signal Start Sync Signals 6 Read Barcode if enabled TRAY Tray Type Barcode None 7 Syringe wash strokes with solvent Pre Cln Slv1 SYRINGE Syringes Fill Speed from Wash Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 8 Syringe wash strokes with solvent Pre Cln Slv2 SYRINGE Syringes Fill Speed from Wash2 Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 9 Rinse syringe with sample selected Pre Cln Spl SYRINGE Syringes Eject Speed number of times Sample Volume Fill Volume Fill Speed TRAY Tray Type Needle Penetr Pullup Delay Waste Injectors Needle Penetr 10 Wait for timer MinCondTime MinCondTime elapsed to Zero 11 Fill syringe with sample using Sample Volume SYRINGE Syringes Eject Speed several filling strokes aspirate air Air Volume Overfill outside of vial if Air Volume gt 0 Fill Speed Fill Volume Fill Strokes TRAY Tray Type Needle Penetr Pullup Del 12 Move to inject
2. 2 me Figure 28 Demonstrating a possible Inclination of Tray in X Y Z Axes PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation 4 2 3 1 Definition of a Tray Row and Column The PAL System defines Rows and Columns by the order in which samples are treated A Row is not associated with an X or Y axis The definition Is visualized below with Tray Type VT32 and DW96 Calurmn E w a uu 123458 7 8 8 101112 Pe DEJEIEIEJEIEIEIEICIETCH www ponnn e ee z LDLDJJJODDUEBLDLCLD o C gt EJETETEJEJETETETETE mmm BO0OCOCOCOCCOCOC I 000000000000 jeooo Arrn www 00000000000 Figure 29 Tray Type VT32 Figure 30 Tray Type DW96 4 2 5 2 Correction for Sample Position in X Y or Z Axes PAL Firmware version 2 X X The following description of the correction is done by using the path to the Utilities section After selecting the Utilities function Trays verify whether the Tray Type assigned corresponds to the Tray placed into the Tray Holder If this is verified the vial positions can be checked at the three corners of the Tray This is activated by the F5 function key Menu Utilities Tray Select Tray Select Function Key F3 Move to 001 Tray Tray 1l Scroll down 2009 06 16 16 07 20 g o9 eq gs Figure 51 Utilities
3. sampling Start Sync Signal Start a cycle Input from GC or data i f system Drusus 8 GND Start2 Sync Signal Continue the cycle GC Dual for Immediat the second sample Ignore Inject Sync Signal Inject Immediat READY to INJECT Ignore Inject2 Sync Signal Inject the second sample for GC Immediat Dual cycle Ignore Injected Out Signal Activated at the moment the SW Outl 3 sample has been injected 4 Injectd2 Out Signal Activated at the moment the Off second sample has been injected Running Out Signal Active as long as the Job Queue is Off being processed and the PAL is not in an error state 1 If used to synchronize with Sync cable change setting to TTL In1 If an item of the class Sync Signal is assigned to an output signal such as TTL In1 then this TTL contact must be defined as either Active High or Active Low as expected by the HPLC system This is defined in the PAL Firmware class Events Menu Setup Objects Events Select the corresponding item and set the mode accordingly The setting is provided with the schematic description of the LC Sync Cable if a dedicated cable is ordered Normally a non specific LC Sync Cable is supplied that has the PAL and PAL X INTERFACE 1 connector mounted and the HPLC system side has four open leads 68 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYST
4. 29 PAL is a registered trademark of CTC Analytics AG Switzerland syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 10 2 2 Service The following Service items may be changed by selecting the particular Item Item Check Motors Test Head i Test Switches Description Every stepper motor can be moved separately This function is useful to check out a specific motor or to perform an endurance test after a repair The Start and Stop positions of the test path can be selected The motor Current Speed and Acceleration are selectable accept default value to be sure A Pause time can be specified A Cycle Limit and Actual Cycle Counter are useful tools for endurance tests This tests the functionality of the PAL Injection Unit Head Two tests can be activated by the function keys Fl Check Plg The stepper motor of the plunger drive is tested Sensor check F2 Check Z The vial detection sensor and Needle Guide Blocking function Is tested These tests are only required if any erratic behavior of the PAL System is observed In such cases contact a representative of CTC Analytics AG X Limit Actual status of X axis sensor Move the X Y Carriage manually across the limit switch to verify activation of the sensor Y Limit Actual status of X axis sensor Move the Y axis manually to the Y zero point at X axis crossing over the limit switch to ver
5. Protocol If set to Off the IP address is provided by the user see IPAddr1 to 4 The user can ask the network administrator for a suitable IP address If set to On an IP address is dynamically sought throughout the provided network Enter the IP address divided into four blocks example IP default Address 192 168 0 1 SubNet mask for network configuration This parameter is read only no changes possible If Gateway Is used in network the Gateway IP address switch hub has to be entered The unique MAC Address for the PAL Xt System Is read and entered into this PAL xt communication protocol This is a read only parameter field PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation Remarks If serial communication Is selected all other parameters in PAL xf Firmware Communication dass are Ignored It is advisable to use a fixed IP address set the DHCP parameter to Off In this mode the PAL xt System always has the same fixed IP address Troubleshooting is easier for the network administrator See also special remarks below The IP address is a fixed PAL xt System address The IP address is often provided by the network administrator The SubNet mask must be provided by the network administrator The network administrator must be informed about this setting
6. 122 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt PA SYSTEM User Manual Installation and Operation Connector AUXI AUX2 PAL and xt System 20 11 Pin Signal Name 1 2 Motor A1 Wil Motor A2 Za Motor B1 15 14 Motor B2 15 18 19 GND 6 Temp 16 Temp y Sens 17 45V 8 9 Heater 20 10 56V Connector Wash Station PAL x System 2 PWR Out 1 3 PWR Out 2 1 PWR Out 1 4 PWR Out 2 Pin Signal Name 1 PWR Outl 2 PWR Outl 3 PWR Out2 4 PWR Out2 Connector Power PAL and xfSystem 1 36V 3 NC 2 GND Pin Signal Name 36 V 2 GND 3 NC 123 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Connector MODBUS PAL xtSystem Pin Signal Name TXD zs 36 V 8 RXD 9 14 GND Connector LAN PAL x System LED left LED right oe 8 1 Pin Signal Name DIR Primary Function TX Out Transmit Data p TX Out Transmit Data 3 RX In Receive Data 45 Not used Terminated 6 RX In Receive Data 7 8 Not used Terminated Connector Fuse PAL xf System Fuse Type FST 5x20 Rating 6 5A Rated Voltage 250 VAC 124 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 Intend
7. 2009 05 06 16 07 20 Figure 34 Adjusting Column Inclination at Third Tray Corner Position The three corner points are now adjusted for a possible inclination of the Tray in any axis A possible deviation from an ideal axis position for the other vials caused by variance from the horizontal is interpolated by the system without further teaching For routine work using standard vials it is not necessary to compensate for a possible inclination as long as the vial detection is within the Z Tolerance range PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 2 4 Wash Station Wash1 Wash2 Reference Point If positions of other types of Wash Stations such as Active Wash Station have to be taught follow the instructions in the specific Addendum of the PAL User Manual Figure 35 Wash Station Wash1 Wash2 Reference Point For a Fast Wash Station the reference positions are the two holes in the wash vial caps see Fig 35 Wash1 and Wash2 The lower needle guide should be centered in these holes with the bottom of the lower needle guide lightly touching the surface of the Wash Station assembly 4 2 5 Wash Station Waste Waste2 Reference Point Injectors Waste The Waste positions represent an Injector within the PAL software It is defined in the Object class Injectors
8. HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 5 1 1 LC Inj Cycle Method Parameter Description Parameter CYCLE SYRINGE Sample Volume Air Volume Pre Cln Slvi Pre Cln Slv2 Pre Cln Spl Fill Speed Fill Strokes Pullup Del Inject to Inject Speed Pre Inj Del Pst Inj Del Pst Cln Slv1 Pst Cln Slv2 Vlv Cln Slvi Vlv Cln Slv2 Description LC Inj Cycle Selected syringe Selected sample volume Volume aspirated after the syringe needle is moved out of the sample liquid Pre injection syringe wash strokes with solvent from Wash1 Pre injection syringe wash strokes with solvent from Wash2 Pre injection syringe rinse cycles with sample Speed of plunger movement used to aspirate sample Number of filling strokes to aspirate sample Delay time between sample pull up and ejection Name of Injector used for injection Speed of plunger movement used during sample injection Delay time prior to sample injection Delay time after sample injection Post injection syringe wash strokes with solvent from Wash1 Post injection syringe wash strokes with solvent from Wash2 Valve rinse cycles with solvent from Wash1 Valve rinse cycles with solvent form Wash2 109 PAL is a registered trademark of CTC Analytics AG Switzerland system HTS PAL HTX PAL PAL HTS x PAL HTX xt User Manual Installation and Operation 3 2 HTS PAL and xf LC Cut Cycle
9. Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 9 Establishing Communication with Computer The PAL System allows a serial communication protocol with a PC As a new feature the PAL xt System provides LAN communication also referred to as TCP IP or Ethernet The PAL xt System requires a dedicated APR Control xf board and PAL Firmware version 4 1 X or higher 9 1 COM Port Settings The port settings are normally defined in Windows Control Panel Device Manager Ports The standard settings for the serial communication are shown in Figure 47 It is important to understand that the application e g PAL Loader Software does actively set the communication parameters and the baud rate is set for optimized use Communications Port COM1 Properties EA X Y Advanced Restore Defaults Figure 47 COM Port Settings A quick way to check whether serial communication with the PAL System can be established is to use the PAL Loader software For details see Addendum to PAL User Manual PAL Loader Software 71 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation 9 2 Setting up LAN Communication Select the firmware Communication class by the following path Menu F3 Setup Communication Menu F3 Setup Communication See detailed
10. The Kit PMKitDLW includes all parts from the PM Kit but additionally the specific parts for the PAL DLW Option e PNo PAL PM Kit1 Description PM Kit for HPLC technique e PNo PAL PMKitDLW Description PM Kit for HPLC Systems including PAL DLW Option e PNo PAL PM Kit2 Description PM for GC technique Contact your CTC Analytics representative to perform an annual preventative maintenance on the PAL System There are no operator serviceable or replaceable parts inside the power supply ies or the PAL System In case of a failure contact a representative of CTC Analytics 103 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation J Appendices 1 Definition of Terms Job Queue A Job Queue is a list of sample processing Jobs Jobs are executed in the order displayed on the JOB QUEUE menu screens New Jobs may be added to the queue while samples are being processed Job A Job contains the information needed by the PAL to process multiple samples by the same processing steps The elements of a Job are a Method and a Tray that define the location of the samples to be processed For identification Jobs are automatically numbered from 01 to 99 and then restarting with 01 when they are added to the Job Queue Cycle A Cycle consists of the specific operations necessary to process one sample The Cycle operation
11. 9A PA IND TNS UD AA LAS UD N A ZNS UD NA M Pwo ZNS UD Id LNS UD AA LAS UD 38d TNS UD 35d 90 DASD pasues jds LAS UD 3Sd 01 10 L1SD pa lul Isd aq fusd aq lui ad aq lul aid paads palu paads pelu 0 palu 0 peluj a drying aq dying lega p h Sions p SONS 4 p ds pali AON sog BUYD Bid paads j paads 4 paads I paads pelul ds up aid ds up aid Aejaq dniind ZPSHO paads pal ZNS UD ald ZNS UD ald Bn Fy amj LM APSHO paads j IAS UD ald IAS UD aud AAS 40 1 ZA X SHO jag dnjng 9uInjoA Jy aulnjoA Jy sod 1njig JAS 3ul asuy adAy e1j SayONS Il4 auinjoA ajduiec auinjoA ajdwes a8uu s Ijauag APN Jjouag JPN ij2uag APN auinjo iiid JONIIAS JONIMAS mig eap Tem ATH ver al ewy TDO TDO pA os 559w HI moi wj sm BECEN KEC Co M IAAQ 3JeMUlJI4 SJH nasas Ve Figure 69 HTS PAL Firmware Overview Page 1 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt Installation and Operation User Manual 0 3JS AA 3tuunjoA aullJd aulnjoA aqnj Apjag pela paads pal paads 4 aq dnijng pds pal3 xew pas II4 Xe paads uiw y 8u 7 apps JAS JONI a8uuAS Jong system C 35 CNA 21 ANIA Jjouag IPN Z uonsog A UONISOg X UONISOd INA D1 UM pds pala xew pds i4 Xe Ijauag 3 paaN 7 Uoljisog A UONISOd X uotsog UM Suolnjejs useA P DMOl4 90 LISD 0 10 LISD 90 OllS 01 10
12. Holder typically a Stack or Agitator uses a Path The Wash Station and its positions Wash1 and Wash2 are visible and selectable Details see below The Firmware Objects accessed on the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as Maximum Penetration can be defined and limited for the user so that this value cannot be exceeded In the Tray Types Object details for the various Items are described Furthermore in this Firmware Object dass a Valve and or a Heater can be assigned Note that the Wash Station Positions Waste and Waste2 are listed in this Injectors dass The Valves and Valve Drives connected and controlled by an AUX interface are listed in this Object dass For more details see the Valves and Valve Drives Addendum Note that the valves controlled by serial communication Serial Valves or Multiposition Valves are in the PAL Firmware Object dass Serial Valves 9l PAL is a registered trademark of CTC Analytics AG Switzerland syste Agitators Dilutors Tools Positions Paths Sync Signals Out Signals Events Serial Valves Out Exp Box RS232 HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation H i At this level items such as Motor Drive Minimum Speed and Maximum Speed are selectable for the Agitator Object The range can be set for the speed The other ite
13. Injection Valve 58 6 1 Valve Drives and Valves General Remarks 58 6 2 Injection Valve Flow Path 59 7 Interfacing the HTS PAL to Other Devices 67 7 1 Synchronization and Output Signals 67 8 PAL Software Program 69 8 1 PAL Loader Program 69 8 2 PAL Object Manager Software 70 9 Establishing Communication with Computer 71 9 1 COM Port Settings 71 9 2 Setting up LAN Communication 72 9 3 Troubleshooting for Serial or LAN Communication 75 10 Special Functions 76 10 1 How to Access Extended User Mode 76 10 2 Section Extended User Mode or F3 Setup 77 10 3 Section F3 Setup Objects 8 PAL is a registered trademark of CTC Analytics AG Switzerland system G Troubleshooting PAL and PAL xt System H Replacing Parts 1 MOTIO Board 2 CPU Board 3 Control x Board 4 Injection Valve and Valve Rotor 4 1 Injection Valve 4 2 Replacing Valve Rotor 5 Injection Unit 6 Wash Station I Maintaining the PAL System J Appendices 1 Definition of Terms 2 Naming Convention 3 HTS PAL and xt Injection Cycles 3 1 HTS PAL and xt LC Inj Cycle 3 2 HTS PAL and xt LC Cut Cycle 4 PAL Firmware Overview HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 89 9 91 93 94 95 95 98 102 102 103 104 104 107 108 108 110 113 4 1 HTS PAL Flow Chart based on PAL Firmware Version 2 5 X 113 4 2 PAL HTS xt System Flow Chart based on 4 1 X 5 External Connector
14. PAL HTX xt PA SYSTEM User Manual Installation and Operation 3 Control xt Board PNo APR Control xt The Control xt board combines the MOTIO and the CPU boards in a single control board This board is required for the PAL HTS xt System and operates only at PAL Firmware version 4 1 X or higher 1 Follow the sequence shown in Figure 58 to detach and release the Control x board from its position inside the X axis As shown remove the dummy cover first 2 Carefully pull the two ribbon cable connectors upwards to free them from the board 5 nstall the replacement board in the reverse order After reconnecting the two ribbon cables to the board ensure that the cables are folded flat and can slide inside the X axis without damage Figure 58 Replacing Control X Board MEE PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 Injection Valve and Valve Rotor In this section the commonly used injection valves with 6 ports are described For more details or information on other valve types such as a 10 port valve see Addendum to PAL User Manual Valve drives and Valves 4 1 Injection Valve 4 1 1 Cheminert Type Valve To replace and or clean the injection valve Cheminert type follow the steps shown in Figure 59 Figure 59 Replacing the Injection Val
15. SYSTEM User Manual Installation and Operation 1 Remove the sample loop needle guide fitting and connection tubes from the valve body 2 Unscrew the threaded plastic sleeve at the valve adapter which holds the valve to the valve drive Pull out the valve body from the drive sleeve 5 Checkthat the valve guide pin remains at its position Observe the correct orientation of the guiding pin as shown below in Figure 65 and Figure 66 for the Valve Drive position 4 Install the replacement valve in the reverse order 4 1 4 Rheodyne Valve PD718 313 Diameter Valve Pod 32 mm To replace and or clean the injection valve Rheodyne PD718 3 13 type follow the steps shown in Figure 62 Y Standby or Inject 30 Figure 62 Replacing the Injection Valve Rheodyne PD719 313 1 Remove the sample loop needle guide fitting and connection tubes from the valve body 2 Unscrew the threaded plastic sleeve at the valve adapter which holds the valve to the valve drive Pull out the valve body from the drive sleeve 3 Checkthat the valve guide pin remains at its position Observe the correct orientation of the guiding pin as shown below in Figure 67 and Figure 68 for the Valve Drive position 4 Install the replacement valve in the reverse order PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 2 Replacing Valve
16. The Appendices provide useful information such as the Software Flow Chart Definition of Terms Injection Cycle parameter descriptions or the HTS PAL accessories guide PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation E HTS PAL Operating Instructions 1 Using the Control Terminal The following procedures present the key steps required to set up and process multiple groups of samples with the HTS PAL They are intended to provide an overview for new users and a reminder for infrequent users The HTS PAL and all accessories should be installed with Objects defined correctly A syringe of the specific type called for by a particular method should also be installed Figure 1 illustrates the PAL control terminal and the conventions used to enter edit and view information JOB QUEUE ACTIVE Menu Title Job 03 Stk1 01 001 096 Curser bar highlights an item in list 04 2ml1 001 013 Stk1 01 Spl 001 8 09 M Function Key Definitions Function Keys Press STOP to abort operation Press the inner knob to select an item highlighted by the curser bar Press again to ENTER the selection Rotate the outer knob to move the curser bar Press ESCape to return to the previous level Figure 1 PAL Control Terminal and Conventions 1 1 Menu Screens Different menu screens are displayed depending on the HTS PAL operating stat
17. contain the actual data such as X Y Z positions The data are stored in a flash memory backed up by a battery To optimize RAM and Flash memory use a certain section of memory has been reserved by the software for each of the Objects and Object Items The percentage shown in the Info section provides an indication as to how much of the reserved software space is still available 7 Setup Functions The Setup functions selectable from the Menu screen allow access to various functions for the PAL System The Sound Time and Objects are basic functions used at installation or if changes have been made over time 26 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Menu Setup 2009 05 06 16 07 20 Figure 7 Selecting Setup Functions 7 1 Sounds Item Description Message Box A specific dual beep tone signals that a pop up window Message Box for user intervention appears on the screen This beep signal can be turned on or off Warn Move A beep sound is heard at the start of the PAL movement It is advisable to keep this function turned on for safety reasons End Cyde A beep sound is heard at the end of a cyde Select as desired End Job A beep sound is heard at the end of a cycle Select as desired 7 2 Time Item Description Year The year can be set for the PAL internal clock Month The month ca
18. in different variations are required and can be added De PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation 1 1 Specifications 1 1 1 Sample Capacity e 98x2 mL vials per Tray e 200x1 mL vials per Tray e 32x10mL 20 mL viaals per Tray up to three Trays can be configured 108 x 2 mL vials per Stack Cooler 2 DW 2 x 54 vials 2 mL 216x2 mL vials per Stack 4 DW without cooling heating 2 Micro Deepwell plates per Stack Cooler 2 DW 4 Micro Deepwell plates per Stack 4 DW 6 Deepwell or microtiter plates in Stack or Stack Cooler 6DW or 6 x 54 vials 2 mL e 12 Microtiter plates in a Stack or Stack Cooler 12MT 1 1 2 Thermostatted Sample Tray e Tray Cooler 4 C 70 C e Stack Cooler 2 DW 4 C 40 C 1 1 3 Syringe Sizes e 10 25 50 100 250 500 1000 2500 5000 uL 1 1 4 Liquid Injection Range 10 uL 100 uL with standard 100 uL syringe Down to 0 1 uL with solvent sandwich technique Down to 0 5 uL with optional 4 port internal loop valve Up to 5000 uL with optional larger syringe and loop 1 1 5 Replicate Injections e 1 99 from one vial 1 1 6 Minimum Sample Volume e 1 uLfrom a3 ul sample in 1 mL tapered micro vial in liquid injection mode 1 1 7 LC Injector e Electrically actuated fast switching Valve Drive 6 port Injection Valve VICI Valco Cheminert C2V 1006 CTC standard valve other valves
19. A Never try to repair or replace any components of the instrument that are not described in this manual without the assistance of a CTC Analytics representative There are no operator serviceable or replaceable parts inside the power supply ies or in the PAL System If a power supply is not functioning contact a CTC Analytics representative A The power supplies for the PAL Instrument the Stack Cooler DW Stack Cooler MT and Tray Cooler have the symbols 1 0 on the label for the power switch to switch ON OFF Any additional power supply for other devices such as a Valve Module exhibits the symbols shown below on the label for the power switch C Power ON Power OFF The symbols warn the user that in an emergency more than one power supply must be turned OFF or more than one power cord must be pulled from the power supply or from the wall outlet to shut down the complete PAL System If the basic PAL System Is installed then a single power supply only is installed Turning OFF the power supply or pulling this single power cord in an emergency case will stop the complete PAL System If a Stack Cooler DW Stack Cooler MT or a Tray Cooler is installed in combination with a PAL System then a second power supply is installed in the complete system Turning OFF the power supplies or pulling the two power cords will stop the complete PAL System in an emergency It is important that the power supply ies be in a location where t
20. Another term often used for Job in the chromatographic field is sequence If more than one Job is prepared the term Job Queue is used Before a Job can be activated the operator must verify that the Tray Type matches the specified Tray and vial size type This step is done in Utilities class Menu Utilities Tray select Tray Scroll down 2009 05 06 16 07 20 Figure 3 Verifying Tray with corresponding Tray Type Select the corresponding Tray Type By activating function key F3 the injection unit can be moved to the first position and to another two corner positions to verify the correct selection of the Tray Type and teaching position 5 1 Building and Starting a Job Queue 1 Power up the HTS PAL The JOB QUEUE screen is displayed JOB QUEUE 03 X Stk Ol 001 096 04 2ml1 001 013 Method A Menu Add Del Start Job Job Figure 4 Example of Job Queue Screen PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 2 Load a sample Tray onto an available location in a Tray Holder or Stack Note the corresponding Tray name 3 Add a new Job for the Tray Press the Add Job key to bring up the default Job 4 For TRAY select the Tray name e g stk1 01 that corresponds to the location of the Tray that was Just loaded 5 Enter the First and Last sample number for this Job 6 Select and enter t
21. Figure 36 LC Valve and Waste Reference Position 54 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation For an LC Valve the reference position is the valve needle guide fitting mounted on the top valve port see Fig 56 The lower needle guide of the Injection Unit should be centered in the valve needle guide fitting Adjust the Z Position so that the bottom of the lower needle guide just touches the surface of the valve needle guide fitting Then reduce the value by 2 0 mm See Figure 57 cii 2 mm gap Figure 37 Positioning Z Axis Needle Guide on Valve Needle Guide For the Waste Port the reference position is a hole slightly larger than the needle guide is placed in front of Wash2 see Fig 36 The lower needle guide should be centered in this hole with the bottom of the lower needle guide lightly touching the surface of the Wash Station assembly To adjust the Valve Needle penetration value see below point 6 2 6 Injection Valve Needle Penetration 5 Syringes 5 1 Selecting Syringes The HTS PAL is shipped with 1 PAL SyrLCX100ul Kit LC Syringe X Type 100 uL for HTS PAL consisting of pc syringe adapter 1 pc plunger holder 2 pc syringe SYRX G100 22S 3 The SYRX G100 22S 3 Syringe gastight 100 uL for HTS PAL equipped with 22S Gauge needle is appropriate for injection into the standard CT
22. Function Trays PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation The Injection Unit moves to Tray position no 001 With the help of the inserted syringe is it possible to check the optimized position of the needle tip matching with the vial or well position In case the position is off make corrections in X Y or Z positions Continue with function key F5 to move to the next corner to verify this position If the position matches continue with function key F5 to move the unit to the third corner position If a deviation of the ideal position is observed in second or third corner the corrections have to be made on the position no 001 The offset is bound to position no 001 Activating one of the parameters Offset X Y or Z axis at the positions of second or third corner can bend the syringe needle 4 2 5 5 Correction for Inclination in X Y or Z Axes PAL Firmware version 4 1 X or higher The following description of the correction is done by using the path to the Utilities section After selecting the Utilities function Trays verify whether the Tray Type assigned corresponds to the Tray placed into the Tray Holder If this is verified the vial positions can be checked at the three corners of the Tray This is activated by the F5 function key Menu Utilities Tray Select Tray Select
23. HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Figure 19 Installing a Microplate Stack with 3 or 6 Drawers Installation of Stack and Tray Cooler is described in detail in the Addendum to the PAL User Manual Stack and Tray Coolers 2 Carefully lift the X axis assembly on top of the Stack with the mounting clamp teeth fitting into the grooves on the bottom of the X axis 3 Be sure that the clamps fit completely into the grooves Alternately tighten the two Torx screws until the two mounting clamps are firmly in place 4 Double check whether the Stack clamps are correctly attached to the X axis see Fig 9 3 2 5 Installing a Tray Holder 1 Ifa Tray Holder was shipped with the HTS PAL loosen the two Torx screws on the two mounting clamps located on top of the Tray Holder legs Figure 20 Installing a Tray Holder 43 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 2 Install the Tray Holder with the mounting clamp teeth fitting into the grooves on the bottom of the X axis 5 Besurethat the clamps fit completely into the grooves Alternately tighten the two Torx screws until the two mounting clamps are firmly In place 4 Double check whether the two Tray Holder clamps are correctly attached to the X axis see Fig 9 44 PAL is a registere
24. Installation and Operation 4 2 4 Replacing Rheodyne PD718 313 Type Valve Rotor To replace and or clean the valve rotor of a Rheodyne PD718 313 type valve follow the steps shown in Figure 62 The Rheodyne Valve PD718 313 can be maintained in a similar way as the Cheminert valve type Replacing the rotor has to be done carefully as it is possible to insert it incorrectly upside down Below the correct orientation of the Rotor quide pin is illustrated Rotor position view from backside of valve body actuator side Standby or Inject 30 Active or Load 30 Figure 67 Valve Position Standby and Active PD718 313 Valve drive Actuator View from front of Actuator N Figure 68 Actuator Position Standby and Active PD718 313 101 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 5 Injection Unit Follow the sequence of steps shown in section F Description and Installation point 3 2 Assembling the HTS PAL 6 Wash Station Follow the sequence of steps shown in section F Description and Installation point 3 2 Assembling the HTS PAL 102 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation I Maintaining the PAL System Regularly performing
25. ORS CL bns 01 10 UIS wi addy Aes Ton el SAK 6002 aun ZSZ a3JeMulllJ 5 UOISIA3N WA SAS T d JENPIAIPU upea JO UONEINSyUOD aeMpueu y UO spuadap 1no e au M IAJ AO Y ui UMOYS se Palgo Alana apnpur JOU SBOP aJEMYOS pJepuegs au 9J0N PORE UST i399 Po o o C m 7 uonisod A UOnISOd X UONISOd UpIHL seppur en Spuo as Sa nullA SInOH yuow jo Aeq sp lqo SIH naus I Figure 70 HTS PAL Firmware Overview Page 2 PAL is a registered trademark of CTC Analytics AG Switzerland PAL HTS x PAL HTX xt HTS PAL HTX PAL User Manual puepazyMs ny son amp jeuy LD Jo xreuopen paasa P S Wd ayes Jeny 3jeis5 apy amp aows 3jeis jenpy 3jels ay Lno MS ayes jenpy aui e unoqaq SEIS any zuado Installation and Operation 3j8lS jenpy aui exunoqa LIS AY 3jels jenpy aes ANDY gno ajeis jenpy 3jels apy Qno apis jenpy apis apy aes jenpy awl unod 3jels Apy u 1LL ES jenpy awl auNogaq g1dxq NO 3jeis IAY a UL L UN uonsog uo ates ony oH ues 3jeis jenpy uorsog HO aui a2unoqa au asing aw Sunpoig 3 e1S 3N py UOHISOd XBW VIS NPY uoneunsaq INOS OANA uH C eH oons xog dg no sanen leuas swang Sjeusi DUAS Figure 71 HTS PAL Firmware Overview Page 3 syste PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PA
26. Remark Position 1 is the only correction point The Y offset is defined as the distance from the center of the teaching position from the Tray Holder to the edge of the Tray in Y direction Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 584 installed in a Stack Remark Position 1 is the only correction point The Z offset is defined as the distance from the plate surface of the Tray Holder to the teaching point black needle guide flush underneath plate of Tray Holder which corresponds to plate thickness of Tray Holder Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 384 installed in a Stack Remark Position 1 is the only correction point The Path Offset X can be specific for a Tray adjustment in X direction Expressed in mm Remark This item is only valid if a Path is assigned to the Tray Holder as specified in this combination The Path Offset Y can be specific for a Tray adjustment in Y direction Expressed in mm Remark This item is only valid if a Path is assigned to the Tray Holder as specified in this combination The Path Offset 7 can be specific for a Tray adjustment in Z direction Expressed in mm Remark This item is only valid if a Path is assig
27. Rotor 4 2 1 Cheminert Valve To replace and or clean the valve rotor of a VICI Valco Cheminert type valve follow the steps shown in Figure 63 Figure 65 Rotor Replacement of Cheminert Valve Type 1 Do not remove the valve from the valve drive For reasons of improved access all tubing or the loop may be removed 2 Unscrew the two hex screws which hold valve stator to the valve body 5 Lift out the rotor disk carefully with a protected tool tips protected to avoid scratching the surface 4 Insert the new rotor The positioning tabs are size coded Insertion upside down is not possible 5 Tighten the hex screws carefully and evenly to reach a parallel seat of valve stator and body 98 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 2 2 Replacing W Type Valve Rotor To replace and or clean the valve rotor of a Valco W type valve follow the steps shown in Figure 64 P O Enlargement from the back Figure 64 Rotor Replacement of W Type Valve 1 Do not remove the valve from the valve drive For reasons of improved access all tubing or the loop may be removed 2 Unscrew the knurled pre load assembly Do not tamper with the preset socket adjustment screw 3 Engage the end of the rotor with a magnet or a pair of tweezers tips protected to avoid scratching the surface 4 Locate the
28. SYSTEM User Manual Installation and Operation 6 2 5 Valve Needle Guide and Needle Seal All valves are equipped with a special valve needle guide fitting This fitting has a wide diameter to mate with the syringe needle guide on the injection unit The valve needle guide also has a countersunk hole to facilitate insertion of the syringe needle into the injection port Figure 44 shows the example of the setup with the standard valve Cheminert type is AT om Valve Needle Guide E Valve Needle Seal d 1 SN x AN y Figure 44 Valve Needle Guide and Valve Needle Seal The valve needle guide holds the needle seal The needle seal which is a short length of FEP tubing forms the seal around the syringe needle A stainless steel ferrule is tightened around the FEP sleeve to ensure a leak proof fit To ensure reproducible sample injection and minimize carryover it is critical that e The valve needle guide and the valve needle seal be installed properly e he valve needle seal be changed on a regular interval details see below e he needle penetration depth be set accurately see point 6 2 6 Injection Valve Needle Penetration To specify a limit number of maximum penetrations into the Needle Seal is not practicable It is advised to check the tightness of the seal daily or weekly when first using the PAL System to gain experience with the specific use in the laboratory related to the application
29. Setup by pressing the center of the selector knob In this PAL User Manual or in technical notes only the following will be referred to Menu F3 Setup PAL is a registered trademark of CTC Analytics AG Switzerland 76 HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 10 2 Section Extended User Mode or F3 Setup The path to access this section is shown below This Setup section at the Extended User Level provides various options to access other classes which are not visible at the User Level Some classes are known but may show some new items Details are described below Menu F3 Setup Ident Access Sounds Time Scroll down 2009 05 06 16 07 20 Home FE Figure 51 Selecting Setup in Extended User Mode Object Description Ident The Identification of the system provides the opportunity to assign Users site and system names The serial number of the PAL System can be entered at this level Remark After loading a fresh PAL Firmware Backup file the SNo is displayed as XXXXXX Access Access can be selectively opened or limited to different users for the Job Method or Setup Sounds The Beep sound can be adjusted to specific needs For safety reasons It Is advisable to keep the default settings especially the Warn Move turned ON to signal the start of the PAL System to the user Time The PAL internal
30. Types Tray Holders Scroll down 2009 05 06 16 07 20 Home Apis wl Figure 52 Selecting Objects in Extended User Mode Description At this level all loaded syringes are selectable At the Utilities user level only the active syringe Is visible The Firmware Objects accessed on the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as e g Maximum Penetration can be defined and limited for the user such that this value cannot be exceeded In the Object Tray Types details for the various Items are described more closely The object dass Tray Type is only accessible via the PAL Extended User Level All loaded Tray Types are visible and selectable Details see below The Firmware Objects accessed on the PAL Extended User Level have more specific items selectable than at the User level Depending on the type of Tray Holder e g an Access and Restore Path or a Heater and Agitator motor Is assigned Typical example the Tray Holder Agitator All loaded Trays are visible and accessible A Tray must be assigned to a Tray Holder A TrayTypeGroup identification checks if the specified Tray Type is allowed in this combination The offsets X Y Z and the inclination corrections for a row or column are explained in the Utilities Functions section The Path Offset X Y Z is used only if a Path is assigned to the specified Tray
31. Valve Drive s and Wash Station are properly mounted to the PAL X axis The following description is an example of how to teach the reference position for a Tray Holder The described procedure Is common to all HTS PAL Objects Figure 23 Object Reference Position For a Tray Holder the Reference Position is a hole slightly larger than the lower needle guide in the base plate of the holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate see Fig 23 1 Switch ON the PAL power supply 2 Observe the keypad display The model name HTS PAL will display along with the software version number The Job Queue menu screen will then display 46 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX x PA SYSTEM User Manual Installation and Operation 3 When the Job Queue menu displays complete the following sequence common to all objects Menu Setup Objects Tray Holders Named Tray Holder where Named Tray Holder represents a predefined Tray Holder e g THldr1 or Stack1 Tray Holder THldrl Position X 63 2mm Position Y 178 5mm Position Z 188 4mm Access Path None 2009 05 25 11 20 25 Check Clear Movto Home Pos Pos Pos Figure 24 Menu Screen Object Tray Holder 4 After selecting Named Tray Holder the X Y Z positions associated with the o
32. a local level such as an electrical wall outlet c Pollution Degree 2 This is a measure of pollution on electrical circuits that may produce a reduction of the dielectric strength or surface resistivity Degree 2 refers to normally only non conductive pollution Occasionally however a temporary conductivity caused by condensation must be expected d Moisture Protection Normal IPXO IPXO means that there is NO Ingress Protection against any type of dripping or sprayed water The X is a place holder to identify protection against dust if applicable 50 PAL is a registered trademark of CTC Analytics AG Switzerland system HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 1 4 Physical Specifications Parameter HTS PAL Systems Height Depth Width Weight Requirements 648 mm 25 5 in 385 mm 15 2 in 828 mm 22 6 in 10 kg 22 Ibs without accessories 1 5 Operating and Environmental Requirements Parameter Operating temperature range Maximum relative humidity Bench space Vibration Static electricity 1 6 Sound Pressure Level Parameter Sound Pressure Level Requirements 4 to 40 C 39 to 104 F 75 non condensing At least 24 cm 10 in at the rear Access to power switch es and power cord s Clean level and smooth surface Solid bench plate Negligible Negligible Requirements Measured value 62 dBA PAL System used f
33. a registered trademark of CTC Analytics AG Switzerland 50 note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 2 Injection Valve Flow Path The six port valves are connected to the detection system according the flow paths as shown in Figures 40 to 42 6 2 1 Standard LC Injection Valve Cheminert 6 Port To column Sample loop Carrier mobile phase Figure 40 Flow Path LC Injection Valve Cheminert Type The standard LC Injection Valve is a six port two way switching Valve with a flat rotary plate and has a special needle guide Valco VICI Cheminert Flat Plate Rotary Valve Stator Material Stainless steel 316 Rotor Material Valcon H inert mix polymer Port Size 1 16 inch Fittings Valco 1 16 inch Ferrules and Nuts Actuator PAL Valve Drive Micro Electric Actuator Loop Size 20 uL shipped other loop sizes available Bore Size 0 25 mm Port to Port Volume 320 nL for vertical port 75 nL for standard ports 75 nL for engraving Sample loops of the different valve types are not interchangeable 59 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 2 2 Valco W Type 6 Port Injection Valve Figure 41 Flow Path LC Injection Valve W Type The LC Injection valve Valco Type W is a six port two way switching valve with a conical
34. according to the application requirements Consult Appendices point 3 HTS PAL Injection Cycle Parameters for details on specific items PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation 2 2 Edit View Methods Method parameters excluding Cycle and Syringe can be viewed and changed from the Method menu as follows Complete the following menu selections Menu Methods Method name Enter 1 Scroll to and select the parameter to be changed Assign the new value and press the ENTER key 2 Exit from Parameter List by pressing either the Home function key F4 to return to the top level Job Queue menu or the ESCape key to return to the previous menu 5 Method contents may be viewed from the Job Queue displays by selecting the desired Job pressing ENTER followed by the View Method function key 2 5 Delete Methods Methods can be deleted from the Methods menu Methods in use by an active Job cannot be deleted Complete the following menu selections to delete a Method Menu Methods Method name Del Method PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 3 Job and Job Queue A Job bundles the specified Tray with the designated vials samples and with the Method to run those samples
35. as ng aui Suppojg A Sod Sunyeg ayelS ANY uomisog xe 3yeis ADY uoneunsaq aDnos X sog Sunyeg OAA NA LULL Papel aNOgons dS IVN sjeusis INO seudisou s Sf gi dx n0 o suang Figure 75 PAL HTS xt Firmware Overview page 3 system PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt Installation and Operation User Manual system H SIHISAQUUN Tyd ON Hed 0 Aejag IN 1 uuinjo uogipuoo z uuinjoo 3urpeo awn ep o aye 3 880 IALA Z uuinjo uonipuoo uunjoo Suipeo suuinjo eon A eue z quA anea uod or z sjduex3 0 lt ejag IND uuuinjoo PonAjue oj Sutusn ppeq dn ueap ajduies sO uno 3Jg 3 durex3 IAEA SUIDAS z BANA JT dooy yum anea uomealuy 9NeA 21 SaNPA Z sjouo ap 115 5 APA puz uo uuinjoo ad uonipuoo 0 papaeu awy eur Suiuonipuo untur aul PUOUIYY Jopajap 20 ULUNJOD je Ajeue oj ajduues usn ppeq o yams dn ueap J0j papeau atur Aejaa mj APA Sunpiws aea puz jo aweU Ajpads C NA o1 3 A 1 0 TNS UD NA C LAS UD AYA 0 NS U Sd t INS Up 15d uuinoo ean jeue Jo asd ayen puz o3u0 do07 woy ajduues Jajsue 0j papaau atu Aeja saysues dues jaqysued jd sw 00S pa lu sq SW 00S ed ful aug s m oL paads paluy LAADI 0 palu Sort ag anjing S YONS li s m ol paads Ilid c 105 U ad 0 CNS Ul Ad l IAS Ul 94d ju o UNJA INY mog auno ajdwes iri 001 JONIYAS m N FDA 122
36. can be hazardous and flammable Acidic vapor phase can cause corrosion to critical mechanical parts Disposal A Do not dispose of this equipment or parts thereof unsorted in municipal waste Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment WEEE European Union customers Call your local customer service representative responsible for the PAL System for complimentary equipment pick up and recycling PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Commonly Used Symbols A Caution Risk of Needle Stick Puncture m oem 000 Fuse mE Used with Main PAL Power Supply 0 Used with Main PAL Power Supply O o o Used with Optional Device s EHE M Optional Device s Caution Risk of Electrical shock high voltage Disposal Do not dispose in municipal waste Follow local waste regulations to reduce electrical and m electronic waste WEEE PAL is a registered trademark of CTC Analytics AG Switzerland system B Table of Contents A Safety Information B Table of Contents C List of Figures D How to Use this Manual E HTS PAL Operating Instructions 1 Using the Control Terminal 1 1 Menu Screens 1 2 Function Keys 1 5 ESCape and STOP Keys 1 4 Scroll Knob and ENTER Button 2 Methods 2 1
37. its position inside the X axis As shown remove the dummy cover first Slide the MOTIO board to the left side This is necessary because the board mates on its right side with the connector on the CPU board 2 Carefully pull the two ribbon cable connectors upwards to free them from the board 3 Install the replacement board in the reverse order After reconnecting the two ribbon cables to the board ensure that the cables are folded flat and can slide inside the X axis without damage 9 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt PA SYSTEM User Manual Installation and Operation Figure 56 Replacing MOTIO Board o M PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 2 CPU Board PNo APR CPU 1 Remove the right end cover of the X axis facing the rear of the chassis Remark This step is only necessary if the CPU board needs to be removed and the MOTIO Board remains in the system 2 Slide the CPU board out and towards the right end of the X axis This is necessary because the CPU board mates on its left side with a connector on the MOTIO board 3 Install the replacement board in the reverse order Figure 57 Replacing CPU Board PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x
38. maintenance helps ensure accuracy and precision of the PAL System Suggested intervals for maintenance procedures are given below to ensure uninterrupted operation If you use the system extensively for example nights and weekends or if you use corrosive solvents you may need to perform the maintenance procedure more frequently Maintenance Step Interval Clean the outside of the instrument Weekly or as needed Use only a soft lint free cloth dampened with mild soap and water Clean Instrument syringe and surfaces Weekly or as needed Replace the syringe plunger Syringe Plunger for a gas tight syringe polymer tip has to be replaced on a regular basis The interval is highly dependent on the application throughput quality of sample solution particles etc washing solvent It is advisable to check the tightness of the plunger on a regular basis to gain experience with the application Check the tightness of the needle in the needle Replace the Valve Needle Seal seal See section F point 6 2 5 Annually or more often depending on the Replace the Valve Rotor throughput and quality of sample solution and mobile phase Wash and Waste Solvent reservoirs Weekly or as needed Replace the Septum as well to avoid carry over The other steps are described in the Preventative Maintenance Procedure Specific kits for the GC or the HPLC techniques are available The kits include the parts which must be changed annually
39. necessary to correct the problem will be sent to user s location shipping costs at previously agreed conditions 55 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation What will CTC Analytics NOT Do A service representative will not be sent to perform service at customer s location prior to a determination by the responsible CTC Analytics representative that such is necessary Only after telephone support and replacement of any modules sent to user s site have failed to resolve the problem will we send a service representative on site CTC Analytics AG will not replace consumable parts as explained above How can you get Technical Assistance To obtain technical assistance call the responsible CTC Analytics representative from whom the instrument was originally purchased Please be prepared to provide the serial number of the instrument and discuss the problem in detail and be willing to perform recommended tests and adjustments to help us determine the probable cause of and solution to the problem What must you do to keep the Warranty in Effect To keep this warranty in effect the user must take care to avoid accidents misuse and abuse as described above under What is Not covered by this Warranty The maintenance guidelines outlined in the PAL User Manual must be followed Preventative Maintenance PM or any
40. on if you suspect that it has incurred any kind of electrical damage Instead disconnect the power cord and contact a CTC Analytics representative for a product evaluation Do not attempt to use the instrument until it has been evaluated Electrical damage may have occurred if the PAL System shows visible signs of damage exposure to any liquids or has been transported under severe stress A Damage can also result if the instrument is stored for prolonged periods under unfavorable conditions e g subjected to heat water etc Ensure that the power supply controller unit is always placed on a dry and clean position Avoid any spill of liquids A Always disconnect the power cord s from the power supply or from the various power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still be charged even if the instrument Is turned off To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the PAL system Once the power is turned off wait approximately 50 seconds before you disconnect an assembly PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation The instrument includes a number of integrated circuits These circuits may be damaged if exposed to excessive line voltage fluctuations and or power surges
41. range expressed in mm where the Injection Unit has to expect an object If the value of this item is gt 0 this is a relative detection mode and the sensor from the Injection Unit needle guide Is active If the value of this item Is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object Z Retract The distance to lift up the syringe slider before the Y Z axes assembly moves Max Penetrat Needle Penetr across the unit can be specified expressed in mm The maximum allowed Needle Penetration can be defined as a safety so that the user cannot destroy the needle tip by going too far down The maximum needle penetration must not exceed the length of the syringe needle considering the loss due to needle guide etc Typical needle length is 5 mm maximum penetration must not exceed 48 mm This item is identical to that at the User level The value defined at this level is mirrored to the Extended User Level Type The specific mode for the Wash station in use has to be defined Standard Standard Wash Station Syringe is filled with wash solvent and expelled into Waste Pulse A pulsed signal is sent to activate the device Used for the Fast Wash Station and Active Wash Station See Type Active for details Flow Continuous flow of liquid by gravity No electrical contact required for a solenoid DLW Dynamic Load amp Wash It
42. the last six characters of the MAC address which is known from the parameters MacAddr4 MacAddr5 and MacAddre for the PAL System If a MAC address only consists of one character insert a O in front of it If for example Maxaddr4 BA MacAddr5 35 and MacAddr6 3 enter CBA3503 and not CBA353 9 3 Troubleshooting for Serial or LAN Communication Both communication protocols are described in detail in the Addendum to PAL User Manual PAL Loader Software 75 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 10 Special Functions This section describes special functions which can be activated by the PAL Extended User Mode The purpose of having two software access levels is to display discrete items and sections of the firmware at each level Settings which need to be done rarely perhaps at the time of installation of the PAL System are hidden at level 1 User Level and revealed at level 2 Extended User Level This also protects the PAL System in group use Nevertheless the important points should be known by the user and are described below 10 1 How to Access Extended User Mode Extended User Mode can be accessed by the following path Menu F3 Setup The above path as shown is an abbreviation for the following steps e Menu scroll the cursor to Setup e Press function key F3 e Activate
43. tubing into a waste reservoir not supplied which is positioned lower than the Wash Station assembly Stretch the PE tubing to make sure that no used wash solvent is trapped before the Waste Reservoir note The waste reservoir MUST be placed in a lower position than the Wash Station assembly 4 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 8 Connect the control cable between PAL connector INTERFACE 2 and the four pronged connector located on the Fast Wash Station assembly See below Figure 18 and for more details Figures 21 22 For a PAL HTS xt the cable can be connected either to the INTERFACE 2 connector or to the small round connector labeled Wash Station Both connectors provide the same functionality The cable determines the connector Figure 18 Electrical Connection Fast Wash Station If other types of Wash Stations are installed such as Active Wash Station follow the instructions in the specific Addendum of the PAL User Manual 5 2 6 Installing a Microplate Stack 1 If a Stack a Tray Holder with multiple drawers was shipped with the HTS PAL loosen the two Torx screws on the two mounting clamps located on top of the Stack The example below shows the two stack models with 3 and 6 drawers 42 PAL is a registered trademark of CTC Analytics AG Switzerland note
44. tuning the Offset items from the class Trays can be used 85 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Explanation for various Patterns in Tray Type 1 Pattern Type Regular The sample or well positions are equal distributed i u II V a up 12 3 4 5 6 7 B 2 10 1 12 loooe LIICIEIGIEIEIEIEJERETEI e www cs D00 e s LILILILILLDLDQOLBLBLOL loeee HOOD SSoooo um OOit 000 OOOO logge DODO SSooooo www LICIEIEIEIEREIEIEILEGIGIG Figure 53 Pattern Tray Type Regular 2 Pattern Type Stagerd The sample or well positions are arranged in a staggered pattern Offset of second Row Is shifted by 50 of hole pattern Column Ko z we t er c Row 123456789 101112 A tm A m s B o f em c 5 a m 8 5 t M F om Tl m D G Figure 54 Pattern Tray Type Staggered 3 Pattern Type Staggrd The sample or well positions are arranged in a staggered pattern Offset of second Row Is shifted by 50 of hole pattern Column E f gt z ow e
45. with solvent from Wash2 Pre injection syringe rinse cydes with sample Speed of plunger movement used to aspirate sample Number of filling strokes to aspirate sample Delay time between sample pull up and ejection Name of Injector used for injection Speed of plunger movement used during sample injection Delay time prior to sample injection Delay time after sample injection Time needed to transfer sample from Loop onto 2 valve pre or analytical column Post injection syringe wash strokes with solvent from Wash1 Post injection syringe wash strokes with solvent from Wash2 Valve rinse cycles with solvent from Wash1 Valve rinse cydes with solvent form Wash2 Specify name of 2 valve switching valve Time needed for clean up Switch to backflush sample to analytical column or detector Minimum Conditioning Time Time needed to condition pre column on 2 valve 112 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL 6 s 5 gt lt m D I A O c g 7 O O B oc SES rs i e 2E aD E system 4 PAL Firmware Overview 4 1 HTS PAL Flow Chart based on PAL Firmware Version 2 5 x 6002 aunr ZSZ aJeMui4 5 UOISIADY ulajs s 1yd enpiwpur upea 10j uonein8ijuo arewpaeu ay uo Spuadap jno e au MAIAJBAD IY ur UMOYS se Palqo A epnpur JOU SBOP aJewjos pyepuejs y e ueugjurejW ojuj nuew J9 uno UMS AIPA pue axONS JABUNId SIJON dU PUODUIWY hepa 1
46. 1 SJa aUJPJed Pours Tvd apA SYLW Papuswworsy pourayy ane pue BBULAS ui SJUAAIOS JULIO ajeuluiia JUBAJOS snoanbe YIM usew 31d ayduues xau 310424 apAD useA ISL uaAo5 HUPBIC ap usen puz uaNos snoanby 3pA USeAA ISL sajduies eoi80jorq 10 sdays ysenA SjuaAos IIUPBIO pejuo jou pjnous Suad Sururejuo sajduies sanuejod IUII jus xe jo sjusuodiuo YIM Sa duies 30 Z pue L JUaAIOS ISN SultjsPM ISOg pue ig aBuuAS seninn S T ost 01 05 a3uuAs Trl 001 304 paads p h juanyos jo Aysoas uo spuadap s 1rl ooz 0 dn paads ij swoje zz BN amp zz OOLD X S quanjos jo Aysoosi uo spuadap sT oz s sAT oz xew o paads jy syw szz anen Szz GOLD X JAS a8uu s 1uajuoo doo jo 0508 OZ sdoo 128327 ri 001 5 sdoo 10j uajuo2 doo jo 2609 OZ aues aunoa aydwes pawojry Suny doo jeneg sauun 6 jjuiawo Tri oz azis doo Suomnipuoo SuiMoJjoj aut 104 a duiex3 0 ZAS UD AA 4 LAS UD NA 0 INS UD 8d Z LAS U Sq Su 00S eq fuj Sd SW 00S Jed lul aad sim c paeds palul LAADI 91 palu s OL fag dnijng SAYONS sim c paads j c IGS u 34d 0 TAIS Ul 34d l LAS Ul Bd uo 9unjOA ANY n 08 aunioA ald wes Ti oot JONIMAS lup 51 DAD ur 51 SJ3 3tUPJed POULIN Tyd apA papuetuulo ay potus PAL is a registered trademark of CTC Analytics AG Switzerland Figure 76 PAL HTS xt Firmware Overview Page 4 HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User M
47. 3 Installation 3 1 Unpacking the Components A HTS PAL system is shipped in one box The box contains the X Y axes assembly the Injection Unit the Keypad Terminal standalone Supports connecting cables Power Supply Syringe kit Wash Station assembly Safety Guard and miscellaneous parts All optional parts such as TraySet Stack Cooler second valve drive assembly etc are shipped in a separate box 1 Open the box and first remove the accessory boxes and the Injection Unit before attempting to remove the X Y axes assembly 2 Carefully lift the X Y axes assembly and remove it from the box Hold the Y axis in place while the assembly is being removed from the box Set the X Y axes assembly on a bench 5 Unpackthe remaining small boxes and any other accessories 4 When placing the HTS PAL onto a stable surface make sure that no objects interfere with either the Y axis or the Injection Unit throughout the entire range of potential movement 5 2 Assembling the HTS PAL Before beginning the assembly process determine approximately where the LC injection valve and keypad terminal will be located The terminal may be mounted on either side of the X axis 1 If a Stack a Tray Holder with multiple drawers was shipped with the HTS PAL loosen the Torx screws on the two mounting clamps located on top of the stack 2 Move the X Y Carriage to the center of the X axis and temporarily place the X axis assembly on top of the
48. 5 mm headspace vials 20 mL MT96 Standard 96 position shallow microplate DW96 Deep well 96 position microplate MT384 High density 384 position shallow microplate PAL is a registered trademark of CTC Analytics AG Switzerland 107 system HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 3 HTS PAL and xt Injection Cycles 3 1 HTS PAL and xf LC Inj Cycle No Processing Step Method Object Class Used Object Parameter Value Reference X Y Z axes and syringe System Start Ref ON plunger 2 Wait for start signal Start Sync Signals 5 Read Barcode if enabled TRAY Tray Type Barcode None 4 Syringe wash strokes with solvent Pre Cln Slv1 SYRINGE Syringes Fill Speed from Wash1 Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 5 Syringe wash strokes with solvent Pre Cln Slv2 SYRINGE Syringes Fill Speed from Wash2 Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 6 Rinse syringe with sample selected Pre Cln Spl SYRINGE Syringes Eject Speed number of times Sample Volume Fill Volume Fill Speed TRAY Tray Type Needle Penetr Pullup Delay Waste Injectors Needle Penetr 7 Fill syringe with sample using Sample Volume SYRINGE Syringes Eject Speed several filling strokes aspirate air Air Volume Overfill outside of vial if A
49. 6 Reduce Inject Speed in method Increase method parameter Fill Strokes Check syringe parameter Eject Speed Increase Pst Cln Slv1 Slv2 values in method Use PreCInSlv1 Slv2 and PreClnSpl Syringe pressure differences Increase Pullup Delay value Vacuum created in sample vial Method Parameters Reduce sample volume in sample vial Use setting under F3 Setup System PrePressureVial Check recommended Method Parameters in Flow Chart Firmware Overview Appendices point 4 The following parameters are crucial and should be verified first Hill speed Pullup delay Injection Speed Post Inj Delay 09 PAL is a registered trademark of CTC Analytics AG Switzerland syste Peak distortion or tailing Excessive carryover between samples Unexplained collisions of the Injection Unit Error Message Object Not Detected Error Message Object collision before Z Tolerance Object e g Tray LOVIV1 CStk1 01 Error Message Invalid Motor Status Motor AUX1 AUX2 Method Parameters Solvent for analytical solution not suitable Tubing internal diameter Bad connections HPLC related problems Dirty syringe and or valve injection port HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation See recommended actions above Change solvent composition to a lower polarity 8incase of reversed phase ch
50. Box provides 8 TTL contacts and 8 relay contacts 24 V contact closure The optional module box is connected and controlled by Interface 2 For more details see Out Expansion Box Addendum to PAL User Manual This Object allows selecting the serial ports for the specific needs Choices are Remote Terminal Printer Barcode ExtDev VICI Vlv for serial valve drives or None 82 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Motors Installation and Operation In the class Motors the Objects Motor Y and Motor Z are accessible to enable the user to select specific motor parameters in case a specific application requires an adjustment Motor Y Strategy Three different settings are selectable for the Y Motor movement strategy Rectang normal movement as used for PAL system In firmware version lt 4 1 X this mode was called Auto AY Simul X Y axes move simultaneously which allows movement in a diagonal direction Do not use this mode for routine work At firmware version lt 4 1 X this mode was called Rectang but it was not active at lower versions Retract Y axis moves from any point first to zero Y back to the X axis before the X movement is started This mode allows bypassing an object such as a large GC detector which may be in the travel path if the default mode Rectang is
51. C injection valve However certain applications may require different syringe sizes Consult the PAL Accessories Catalog in Appendices for details 55 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Gauge 22S refers to small internal needle diameter The X type syringe stands for inertness and for a high number of duty cycles 5 2 Syringe Priming It is critical that syringes be primed before beginning sample preparation Prime every liquid syringe first manually before inserting into PAL system Afterwards the syringe can be primed and tested by using the Utility function Clean Syr 5 3 Installing a Syringe Before a syringe Is inserted the position Change Syr should first be verified The position Change Syringe should not be located above an object where the needle could collide with a vial as for example Path Menu Utilities Syringe tunction key F3 Change Pos Move the PAL Injection Unit to a location where a collision cannot occur by selecting the X and Y axes The value for the Z axis is given as a default and a change of this position is not necessary in standard operation 1 Select Menu and press F1 Chang Syr The Injection Unit will move to a location that will facilitate installation of the syringe 2 Place the blue plunger holder into the injection unit 3
52. Creating Methods 2 2 Edit View Methods 2 5 Delete Methods 5 Job and Job Queue 3 1 Building and Starting a Job Queue 3 2 Aborting a Job Queue 5 5 Restarting an aborted Job Queue 4 Utility Functions 4 Syringe 4 2 Tray 4 5 Injector 4 4 Wash Station 4 5 Vial 4 6 Dilutors 4 7 Tools 5 Logfile 6 Info Functions 6 1 Hardware 6 2 Software 6 3 Maintenance 6 4 Free Objects Free Items 7 Setup Functions 7 1 Sounds 7 2 Time 7 3 Objects PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation F HTS PAL Description and Installation 28 1 General System Overview 28 1 1 Specifications 29 1 3 Electrical Specifications 30 1 4 Physical Specifications 5 1 5 Operating and Environmental Requirements 5 1 6 Sound Pressure Level 5 1 7 Hardware and Software Requirements 32 1 8 Regulatory Compliance Requirements 32 2 Product Warranty 33 2 1 Statement of Limited Product Warranty 33 3 Installation 35 3 1 Unpacking the Components 35 3 2 Assembling the HTS PAL 35 3 3 Electrical Connections 45 4 HTS PAL Object Positions 46 4 1 Defining Object Positions 46 4 2 Description of Object Positions 49 5 Syringes 55 5 1 Selecting Syringes 55 5 2 Syringe Priming 56 5 3 Installing a Syringe 56 5 4 Removing a Syringe 57 6
53. EM User Manual Installation and Operation 6 2 6 Injection Valve Needle Penetration Before performing the following steps make sure the Object Positions X Y Z for LC Vivi LC Viv2 are properly defined and a syringe adapter including a syringe Is inserted in the Injection Unit see points above Complete the following steps to define the Valve Needle Penetration Depth 1 In the Object Class Injectors choose the Object LC Vlv1 The following screen Is displayed Menu Setup Obje cts Injector LC Viv1 Injectors Position X 246 9mm Position Y 8 6mm Scroll down Position Z 86 0mm Needle Penetr 20 0mm Valve DC6 1 C 2009 05 06 16 07 20 Check Clear Movto Home Pos Pos Zero Figure 45 Menu Screen Valve Needle Penetration Depth 2 Press the F1 button Check Pos The Injection Unit moves to the previously defined LC Vlv1 position 5 Highlight the item Needle Penetr with the cursor bar and press ENTER 4 Slowly rotate the outer knob to adjust the Needle Penetration Depth The syringe moves down stepwise into the Injection port 5 When the syringe needle tip enters the valve needle guide slow down the Z movement again Always observe the syringe needle during this operational step 6 Move down stepwise until you notice a click sound inside the Injection Unit STOP the Z down movement immediately 7 Rotate the outer knob two steps in the opposite direction and press ENTER to save the Needle Pen
54. EM User Manual Installation and Operation 8 PAL Software Program 8 1 PAL Loader Program The purpose of PAL Loader Software is to create a backup file of the complete PAL System Firmware The core software firmware and all PAL Firmware Objects including all settings Items the local methods and jobs are saved in one file The PAL Loader software has a second function of loading the firmware or a complete backup file to a PAL system The PAL Loader software has always been a part of the PAL System The following PAL Loader Software versions are in use PAL Loader Version 1 0 1 PAL Loader Version 1 1 1 A new PAL Loader software Loader version 2 1 1 or higher has been developed for the PAL X system The PAL xf system requires PAL Firmware 4 1 X or higher which calls for the new Loader software The new PAL Loader Software is backwards compatible The software can be used for all PAL Firmware versions The name of the exe file has been changed intentionally to avoid conflicts when old and new versions are installed on the same computer e PAL Loader version 1 1 1 PALLOAD exe PAL Firmware version up to and including 3 X X e PAL Loader version 2 1 X Setup PALloader exe PAL Firmware version as of 4 1 X but backwards compatible with lower versions Details are described in Addendum to PAL User Manual PAL Loader Software Version 2 1 X Installation and Operation 69 PAL is a registered tradema
55. Function Key F3 Move to 001 A dialog window opens displaying a note to Adjust offset X Y Z for Position 001 Continuing in this dialog will provide the user the opportunity to adjust all three axes perfectly by the top of the vial Please note that this step Is only valid for position number 001 Adjust Offset X Y Z for Position ool 2009 05 06 16 07 20 ie Figure 32 Adjusting Offset X Y Z for Tray Position 001 52 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX x PA SYSTEM User Manual Installation and Operation To check the next corner of the Tray activate function key F3 again example Tray Type VT32 position 008 The next information provided by the pop up window asks whether to correct a possible inclination of the Row Continuing the dialog provides the user the opportunity to adjust the three axes perfectly to match the vial top or in case of a well plate the top of the plate FS Move to 008 Adjust dx dy dzRow for Position Figure 33 Adjusting Row Inclination at second Tray corner position The last step will be to move to the next corner of the Tray by again activating function key F2 This will be position 32 in the example of Tray Type VT32 A possible inclination of the Tray column can be corrected in the same manner as described above Adjust dx dy dzCol for Position 032
56. HOME position and the Job Queue Menu is displayed The following Dilutor items can be changed by selecting the particular item Item Description Syringe Indicates the Dilutor side port syringe inserted in the Z axis This function allows selecting another syringe size Syr Dilut Pos Activating this function moves the plunger of the side port syringe up by the specified distance This allows adjustment of the plunger tip of the side port syringe exactly above the lower side port solvent inlet This fine tuning is necessary after changing a side port syringe to allow unrestricted solvent flow Dilutor Syr Indicates Dilutor Syringe is installed If the syringe size is changed this item must be adapted accordingly The syringe specific dimensions are coordinated by this function Prime Volume A volume to prime the Dilutor Syringe can be specified The allowed range can be fourfold higher than the actual syringe volume This allows filling and emptying a dilutor syringe more often than just once Pullup Delay Using this item permits selecting a delay time between solvent filling and ejection while filling or priming the syringe Fill Speed Speed of plunger movement of Dilutor Syringe used in all syringe filling operations Eject Speed Speed of Dilutor Syringe plunger movement used in all dilutor syringe eject operations Eject Delay Using this item permits selecting a delay time between solvent ejection and filling while filling or pri
57. HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation PAL User Manual Installation and Operation HIS PAL HTX PAL PAL HTS x PAL HTX xt Printing History Edition January 1999 Software Version 2 0 Edition 2 April 2003 Software Version 2 0 Edition 3 November 2009 Software Version 2 5 to 4 1 X Edition 4 March 2010 Software Version 2 5 to 4 1 X Edition5 June 2010 Software Version 2 5 to 4 1 X Original Instructions CTC Analytics AG reserves the right to make improvements and or changes to the product described at any time without notice CTC Analytics AG makes no warranty of any kind with regard to this product including but not limited to implied warranties of merchantability and suitability for a particular purpose In no case shall CTC Analytics AG be liable for any coincidental or consequential damage in connection with or arising from the use of this document 2009 CTC Analytics AG All rights reserved Neither this publication nor any part hereof may be copied photocopied reproduced translated or reduced to electronic medium or machine readable form without the prior written permission of CTC Analytics AG except as allowed under copyright laws CTC Analytics AG acknowledges all trade names and trademarks used as the property of their respective owners PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM U
58. L 6 ag 5 ce 5 z p c a EE A O a 8 C SES reso lU CS coo c I E 0 ejag 1 uuinjoo uonipuon z uuinjo Suipeo awn ap o saye 3 880 ALA Z uuunjo uonipuoo uuimjoo 3uipeo suuinjoo jeon jeue z yya aaea uad or z s duiexg 0 lt Aejag 17 uuinjoo jeonAjeue o Suigsnjppeq dn ueap ajdwes JO uunjo dig t ejduiex3 BALA SUILDIIMS Z INLA N doo ym anea uomelu 34A N SaA P Z SOU APA M J anea puz uo uuinjo2 aud uonipuo oj papaau ai awy Suruogipuo WNwIUIYY 3l DUC UNYY JOPS EP 10 uuinjoo jeon jeue oj ajdwes usnjppeq 0 uis dn uesp 104 papasu aui kepa 1m BALA Sui piis ape puz JO aweu Ajpads CNA 1 Nea 11 0 INS UD NA 4 IAS UD A 0 TNS UD Sd 4 INS UD Bd uwnjo jeonAjeue JO ad anjer puz ojuo doo wos ajdwes Jajsue 0 papaau aui Abjac Jajsues ajdues paues sw 005 pa lul sd sw 005 pa lul ad sjim ot paads p luj LAADI 0 paluj OL E pa dning SOYONS I4 sim ot paads I4 4 ds up ad 0 ZNS UD 94d l LNS U ed uo 3ulnpp Iy mi 08 3unjoA sjdiues oot JONINAS n 1 JAD 521 SJ3 aUUPJed POULIN Tvd apAy syeway papuatuuo 3y poulaw system AAPA pue a3uuAs ui sjusAjos JULBO ajeuiuuia jua jos snoanbe tiM YSeM d1d ajduues Wau 310jaq 3p USPAA 151 JUANOS JULBO ap usea puc Juanjos snoanby 3pA useAs 15 sajduues jeoi8ojoiq 10 SANS UseAA Sjua jos 3ruejo Peruo jou pjnous surajod 8uruiejuoo sajduues sanuejod juaja
59. PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 7 PAL Accessories The accessories for the PAL System are described in the attached PAL accessories Catalog or on the web page www ctc ch 126 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation This page intentionally left blank 1127 PAL is a registered trademark of CTC Analytics AG Switzerland
60. Place the syringe in the appropriate syringe adapter Pull the plunger out to approximately 20 of its length See Fig 38 Figure 38 Syringe and Syringe Adapter for Liquid Injections 4 f necessary loosen the plunger retaining screw in the plunger holder 5 Move the syringe installed in the syringe adapter partially into the Injection Unit First guide the needle into the upper needle guide and then into the lower needle guide See Fig 39 56 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Insert the plunger button into the plunger holder Allow the syringe adapter to click into place by magnetic force against the syringe carrier Tighten the plunger retaining screw against the plunger button Press Home The plunger moves down until it hits the mechanical stop This position is stored as the syringe s zero volume position The Injection Unit then returns to the HOME position Figure 39 Installing and Removing a Syringe for Liquid Injections 5 4 Removing a Syringe l Select Menu and press F1 Chang Syr The Injection Unit will move to a location that facilitates removal of the syringe Loosen the plun
61. Stack with the mounting clamp teeth fitting into the grooves on the bottom of the X axis For HTS PALs without a Stack support the X Y Carriage in a suitable manner a sturdy cardboard box can be used before attempting to install the legs 5 Install the legs near the ends of the X axis Loosen the Torx screws on the mounting clamps and then fit one leg into the grooves in the X axis Be sure that the clamps fit completely into the grooves Tighten the Torx screws until the legs are firmly in place 4 Double check whether the leg claws are correctly attached to the X axis see Fig 9 55 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Figure 9 Attachment of Mounting Claws 3 2 1 Installing the Injection Unit Installation of the Injection Unit should be done carefully When installing it for the first time have someone hold it in place while the mounting screws are inserted D Guide pins Figure 10 Attaching the PAL Injection Unit 1 Remove the three Torx mounting screws A B and C used to fix the Injection Unit to the Y axis 2 Connectthe ribbon cable A protruding from the front end of the Y axis to the corresponding connector on the Injection Unit see Fig 11 56 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM Use
62. The following simple test provides assurance of tightness e Take the Needle Seal out of the valve port e Manually insert a syringe needle gauge 22 or Gauge 22S from top down into the tube passing the sealing point of the ferrule If a restriction Is observed continue to use the Needle seal If not replace the Needle Seal 65 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation 6 2 5 1 Interval to Replace Needle Seal The interval to replace the Needle Seal depends on the number of penetrations Two factors may be considered for the decision to replace the Needle seal e Leak seal not reliable Remove the Needle Seal from the inlet port and insert a syringe manually with the corresponding needle gauge standard Gauge 22 OD 0 72 mm Move the needle below the seal point of the ferrule If no resistance Is observed at this point replace the Needle seal e Carry over In case of any doubt of possible contamination of the Needle Seal with a compound replace the Needle Seal Contamination can occur if the sample solution is accidentally dispensed too fast into the valve system The restriction of valve and loop may cause backpressure and the sample solution can be forced backwards into the inlet port of the valve PAL Firmware version 2 4 X or higher provides a counter for the number of injections performed wit
63. The port must be cleared of firewall protection to enable communication Gateway is used if independent networks need to communicate interactively Communication within the network is provided by a hub or switch defined as Gateway The MAC address is provided by the Ethernet communication hardware The address is also visible beside the read out from the terminal on the label affixed to the Ethernet module on the PCB APR Control X7 74 HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 9 2 2 Special Remarks to the above listed Parameters DHCP If DHCP mode is active an automatic search for the IP address is activated If a free IP address is found the communication between the PAL xt System and the network becomes established The new IP address found through DHCP search is immediately entered into the PAL xt Communication protocol as seen from the local terminal The parameters IPAddr1 to 4 are not updated until one opens the communication menu again The Setup for the PAL Loader software requires entering the IP address or hostname The dynamically provided IP address is displayed on the local terminal As an alternative the following setup procedure can be used Prerequisite PAL xf setting in Communication class DHCP On Open the Setup window and enter the following name in the Hostname or IP Address field CXXXXXX The Xs being
64. and Operation 2 Attach the valve drive to the X axis and tighten the mounting screw To avoid delay volume between sample injection point and detection the injection valve should be located near the detection device 3 Connect the control cable from the valve drive to AUXI The installation of another type or multiple Valve Drive s is described in the Addendum to PAL User Manual Valve Drives and Valves 4 The injection valve and rotor are pre installed on the valve drive 3 2 5 Installing the Fast Wash Station 1 Attach the Wash Station assembly to the Solvent bottle holder Figure 16 Installing the Fast Wash Station 2 Loosen the screw on the Fast Wash Station mounting clamp 3 Attach the Fast Wash Station to the X axis as close as possible to the Injection Valve This will minimize the time required to move between the two positions 4 Tighten the mounting screw 40 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Figure 17 Fast Wash Station Assembly 5 Locate the white Teflon fittings and two lengths of PFA tubing that connect the Wash Station valves to the wash solvent reservoirs 6 Place the reservoirs onto their holders and attach the tubing and fittings 7 Attach one end of the supplied polyethylene tubing to the Wash Station Waste port Place the other end of
65. annot destroy the needle tip by going too far down The Tray Type is prepared for a specific vial size this value is therefore unique to this combination The maximum needle penetration must not exceed the length of the syringe needle considering the loss due to needle guide etc Typical needle length is 51 mm maximum penetration must not exceed 48 mm This item is identical to that used on the User Level or if available as a method parameter of a local cycle The value defined on these levels is mirrored to the Extended User Level The X offset is defined as the distance from the outer edge of the Tray in the X direction to the center of the vial position 1 Measured in mm The Y offset is defined as the distance from the outer edge of the Tray in Y direction to the center of the vial position 1 Measured in mm The 7 offset can be selected for special cases where e g the syringe needle has to reach a deeper point before penetration into a vial A 7 offset value 0 considers the Tray surface or vial top as the reference point Measured in mm Items displayed in italic are available beginning with PAL Firmware Version 4 1 X The dimensions used for Row Column Length or X Y Z Offsets for Sample Number 1 are specific for a particular Tray Type If a general adjustment for a Tray from a specitic vendor e g for a Deepwell Plate has to be made this should be done in the Tray Type class of Object For fine
66. anual Installation and Operation 5 External Connectors for PAL and xt System Connector SERT PAL and xfSystem Pin Signal Name NC RXD TXD DTR bridged with Pin7 Special grounding do not alter GND NC RIS bridged with Pin 4 Do not alter NC NC coO oo o o01 C6X4 h2 Connector TERMINAL or SER3 PAL and xt System Pin Signal Name GND RXD eo o o1 GX h2 zA O Connector SER2 PAL System Pin Signal Name GND RXD TXD NC NC 5V NC GND co o o01 CGX4 h2 12 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Connector INTERFACE 1 PAL and xt System rm pm A f A J J 22220000 Pin Signal Name Pwr Outl 2 Pwr Out2 5 SW Outl NO 4 SW Outl COM 5 SW Out2 NO 6 Opto In1 7 TTL In1 8 GND B Pwr Outl 10 Pwr Out2 11 TTL In2 12 SW Out2 COM 13 TTL In3 14 Opto In1 15 5V Connector INTERFACE 2 PAL and x System 13 O QOOQOOOOoOoOOOooOQ O QOOOOOoOoOoOOoOodOQ 25 14 Pin Signal Name TTL Inl 2 TTL In2 3 TTL In3 4 TIL Outl 5 TTL Out2 6 TTL Out3 7 Opto In1 8 Opto In2 9 SW Outl NO 10 SW Out2 NO 11 Pwr Outl 12 Pwr Out2 13 5V 14 19 GND 20 Opto In1 21 Opto In2 22 SW Outl1 COM 25 SW Out2 COM 24 Pwr Out1 25 Pwr Out2
67. arameters see Appenices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview GC InS Standard cyde covering GC Sandwich technique Details on method parameters see Appenices point 3 Injection Cycles and point 4 Flow Chart Firmware Overview GC Dual Cyde for GC technique which allows injecting two samples in two different injectors The start signal is sent after injection of both sample solutions Details on method parameters see Appenices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview HS Inj Standard cyle for GC headspace technique Details on method parameters see Appendices point 5 Injection Cydes and point 4 Flow Chart Firmware Overview SPME Standard cyde for GC SPME technique Solid Phase Micro Extraction Fiber technique Details on method parameters see Appendices point 4 Flow Chart Firmware Overview TTS The two cycles are dedicated cycles for dissolution applications TTS cycle is used Sotax for transdermal patches and the Sotax cycle is used for tablet dissolution Items displayed in italic are available beginning with PAL Firmware Version 4 1 X Standard injection cycles are selectable and activated directly from the PAL System Any deviation from a standard cycle a Cycle Composer Macro or Cycle Editor ICC CE Cycle must be programmed for customized requirements In PAL Firmware versions lt 4 0 the standard cycles are grouped in Item Cycles
68. are operated with Microsoft Windows Operating systems such as Windows XP and Vista For details see the corresponding Addendum to the PAL User Manual The PAL System can be controlled using PAL control software the Cycle Composer or any data system software that controls the PAL using the Cycle Editor for PAL ICC interpretation e g Analyst ChemStation Empower EZChrom MassLynx Xcalibur For details on software requirements for integration of the PAL System with other data handling systems CDS contact your CTC Analytics representative or the manufacturer s representative of the other CDS software program 1 8 Regulatory Compliance Requirements As of the date of publication this product is compliant with current RoHS and WEEE regulations e Directive 2002 95EC RoHS e Directive 2002 96 EC WEEE CTC Analytics AG reserves the right to make improvements and or changes to the product specifications without notice 52 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 2 Product Warranty 2 1 Statement of Limited Product Warranty What does this Warranty cover CTC Analytics AG warrants only that its products comply with CTC Analytics AG specifications This warranty covers defects in or failures of the Autosampler and major accessories such as Agitator Stack Coolers or Valve Drives occurring as a
69. available on request 1 1 8 Wash Station e Fast Wash Station with two different wash solvents e Other types of Wash Stations are optionally available 29 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation 1 1 9 Standard Configuration e Syringe Volume 100 uL e Loop size 20 uL 1 2 Performance Specification The performance specifications are available as part of a specific separate document 1 5 Electrical Specifications Parameter Requirement Protection class Class Over voltage category Category Il Pollution degree 2 Moisture protection Normal IPXO Voltage 36 VDC Current 3 2A Fuse 16 5 A 250 V PAL System Power Supply Input line voltage Grounded AC 100 to 240 V Input line frequency 50 60 Hz Input power 4A Output voltage 36 VDC Output current 4 16A a Protection Class I Protection dass describes the insulating scheme used in the instrument to protect the user from electrical shock Class identifies a single level of insulation between live parts wires and exposed conductive parts metal panels in which the exposed conductive parts are connected to a grounding system In turn this grounding system Is connected to the third pin ground pin on the electrical power plug b Over Voltage Category II Over Voltage category Il pertains to instruments that receive their electrical power from
70. bject will be displayed see Fig 24 5 Highlight item Position X with the cursor bar and press ENTER The Injection Unit will move to the previously defined X axis position 6 Rotate the outer knob to adjust the X axis position to the Tray Holder reference position 7 Press the inner knob to ENTER the Position X value 8 Repeat steps 5 7 for Position Y and Position Z 9 If the F3 button Moveto Zero is activated the Injection Unit will move to the HOME position 10 Verify the defined X Y Z positions by pressing F1 Check Pos LAT PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS x PAL HTX xt PA SYSTEM User Manual Installation and Operation PAL Firmware version 3 0 X permits correcting for an inclined surface for example a Tray Holder or Tray where the Z axis position in front differs from the rear position For applications such as the PAL MALDI Option perfect alignment of the MALDI tip is vital This compensation for the Z axis position IS provided in the firmware class Tool The release of PAL Firmware version 4 1 X makes possible the correction of all three axes X Y Z in the firmware class Tray The correction of the inclination can be made for a tilted Tray in the direction of the row and or the column Teaching is possible in the Utilities section by using the path Menu Utilities Trays Figure 25 illustrates possibilitie
71. cible sample injections and minimize carry over it is critical that the needle penetration depth be accurately set 4 4 Wash Station After selecting the specific Wash Station the following functions are available Function Key Description F3 Movto Wash The injection unit moves to the selected Wash Station port By selecting the Parameter Needle Penetr on the same screen the Wash Station Needle Penetration value can be checked or changed F4 HOME The injection unit moves to its HOME position and the Job Queue Menu is displayed Item Description Needle Penetr By selecting the Parameter Needle Penetr the Wash Station Needle Penetration value can be checked and or changed Rinse Time If rinse time Is activated value gt 0 the solenoid of a Fast or Active Wash Station opens for the specified time after the syringe needle has been removed trom the wash port after completion of the syringe wash cycle The solvent flows into the wash port without the restriction of the needle be aware of higher solvent consumption The following Wash Station items may be changed by selecting the particular Item Item Description Needle Penetr By selecting the parameter Needle Penetr the Wash Station needle penetration value can be checked and or changed Rinse Time If rinse time Is activated value gt 0 the solenoid of a Fast or Active Wash Station opens for the specified time after the syringe needle has been removed from the wash port a
72. clock can be set Remark If the clock is fast reset all items from Year to Seconds and use the mandatory function key F1 Set Time Communication The communication mode serial or LAN can be selected and conditions are defined For details see Addendum PAL Loader Software System Basic parameters can be predefined for specific needs Details see below Service Basic service tests can be activated Details see below Objects Accessing the PAL Firmware dass Objects in the Extended User mode provides more detailed possibilities for object items At this level an Object can be copied or deleted by using the specific function keys This provides quick access to add e g a second or third injector Items displayed in italic are available beginning with PAL Firmware Version 4 1 X Items displayed in bold are explained in detail below 71 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 10 2 1 System The following System Items can be changed by selecting the particular item Beginning with PAL Firmware Version 4 1 X an intermediate step was added After selecting System the new Settings level follows Activating Settings opens up the described Items below Item Syr Detect Start Ref Stop XY Error PlgPathCheck PlgChnge Pos Init Syr at Inj Signal Description The syri
73. cs AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 2 Methods 2 1 Creating Methods Methods can be defined by the user and assigned names up to eight characters in length Methods can be created copied edited and viewed from the Methods menu Methods can be viewed but not edited from the Job Queue menus Methods are created by either copying an existing Method or creating a new Method Menu Methods Method name Copy Method To copy a Method complete the sequence as follows You will be prompted to enter a name for the new Method Use the scroll knob and the left right arrow function keys F2 and F3 to select among alphanumeric characters and spaces Press the ENTER function key F4 to accept the name To create a new Method complete the following steps Menu Methods Select the insertion point Insrt Method 1 Assign and enter a new Method name as above After a copy of the Method has been created the Method parameters will display and can be edited The Cycle and Syringe entries cannot be changed 2 Ifthe Method is new i e added select and enter a Cycle that is appropriate for the application 3 Select the specific Syringe to be used by the Methoa Once a Method has been created and saved the Cycle and Syringe cannot be changed To use a different Cycle or Syringe a new Method must be created 4 Assign Parameter values
74. d Max Eject Speed 25 Rinse valve inlet with solvent from Vliv Cln Slv1 SYRINGE Syringes Fill Speed Wash1 Inject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 26 Rinse valve inlet with solvent from Viv Cln Slv2 SYRINGE Syringes Fill Speed Wash2 Inject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 27 Wait for timer Cut Delay elapsed Cut Delay These two steps to Zero are executed 28 If Cut Time 0 Cut Time concurrent with the then Cut Valve Injectors wash steps toggle Cut Valve position else Switch Cut Valve to ACTIVE Wait for time Cut Time Switch Cut Valve to STANDBY 29 Move to Home position Home Positions PAL is a registered trademark of CTC Analytics AG Switzerland 111 syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 5 2 1 LC Cut Cycle Parameter Description Parameter CYCLE SYRINGE Sample Volume Air Volume Pre Cln Slv1 Pre Cln Slv2 Pre Cln Spl Fill Speed Fill Strokes Pullup Del Inject to Inject Speed Pre Inj Del Pst Inj Del SplTransfDel Pst Cln Slv1 Pst Cln Slv2 Vlv Cn Slvi Vlv Cin Slv2 Cut Valve Cut Delay MinCondTime Description LC Cut Cyde Selected syringe Selected sample volume Volume aspirated after the syringe needle is moved out of the sample liquid Pre injection syringe wash strokes with solvent from Wash1 Pre injection syringe wash strokes
75. d trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 3 3 Electrical Connections Always switch OFF the PAL power supply before connecting or disconnecting any HTS PAL accessories cable Do not interchange the Terminal with LAN cable connector Before defining the HTS PAL Object positions make sure the LC Injection Valve Fast Wash Station and Keypad Terminal are correctly connected to the HTS PAL X ax s rear side See Figs 21 or 22 Figure 21 Electrical Connections for HTS PAL System AMIN y SS Oo Wi NN AINE DINI ASS Figure 22 Electrical Connections for PAL HTS x System For a PAL HTS xt the cable from the Wash Station can be connected either to the INTERFACE 2 connector or to the small round connector labeled Wash Station Both connectors provide the same functionality The cable determines the connector PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 HTS PAL Object Positions 4 1 Defining Object Positions Remove the Syringe Adapter from the Injection Unit before performing the following steps The objective is to define the Reference Positions for all HTS PAL Objects Make sure the Tray Holder s
76. description below under point 10 The system automatically searches for the MAC address when the Setup Communication window Is accessed MAC address is read from hardware Please wait 2009 04 17 16 07 07 tt E E lt re pa Figure 48 Reading the MAC Address from the Ethernet Communication Port This search for and reading of the MAC address takes approximately 10 to 15 seconds If successful the MAC Address is stored in the system firmware and can be viewed on the terminal as follows Communication Scroll down ud T 07 20 Set Home Comm i Figure 49 PAL Xt Communication Settings H i Pressing function key F1 Set Comm sets the selected communication mode The same is achieved by pressing ESC or Home PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Communication parameters are written please wait 2009 04 17 16 07 20 Set Set Home Comm Deflt Figure 50 Setting Communication Mode PAL is a registered trademark of CTC Analytics AG Switzerland system 9 2 1 Explanations for the various settings for the Communication Protocol Parameter CommPort DHCP IPAddr1 to 4 SubNet to 4 TCPPort StdGateway 1 to 4 MACAddr1 to6 Explanation Setup for serial SER1 or LAN communication Dynamic Host Configuration
77. ed the system takes the injection syringe Eject Speed as defined in the object class Syringe Rinse Time Rinse is the time to rinse the wash port with wash solvent after the wash cyde of the syringe has been finished In the case of Fast Active Wash Station the syringe needle is pulled out of the wash port The wash solvent flows without restriction As a result higher solvent consumption has to be taken into consideration Items displayed in italic are available beginning with PAL Firmware Version 4 1 X 88 PAL is a registered trademark of CTC Analytics AG Switzerland note syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation G Troubleshooting PAL and PAL x System The HTS PAL always represents one of several total components in a chromatography system The following Troubleshooting Guide is limited to the HTS PAL or PAL xt only Symptom or Possible Cause Recommended Action Error Message No or very low Clogged syringe Remove syringe and aspirate dispense liquid manually detector signal is Clean syringe observed Bent needle Inspect and or change syringe Mobile phase and or sample flows out of the wrong ports on LC injection valve Sample is backing up on the valve needle guide Syringe does not fill properly Sample peaks or responses are not reproducible No sample liquid is injected Check and or adjust Needle Penetration in
78. ed Use for PAL System 6 1 Introduction The brand name PAL System represents an entire product family the following description of intended use therefore applying to the product groups PAL and PAL x which include for example the models Combi PAL HTS PAL or PAL Combi xtand PAL HTS xftogether with other PAL PAL xf design dependent subclasses If the PAL System is distributed through OEM partners the product or model name can be replaced with their own name but the Intended Use remains valid 6 2 Intended Use The PAL System is an XYZ robotic system offering high flexibility and functionality Its intended use can be described as being mainly as autosamplers or sample preparation devices for all relevant fields of application in chemical and physical analysis The systems will only be used by trained operators in a professional environment Typical fields of application for the PAL System are e Environmental analysis e Quality control in the chemical and pharmaceutical industries e Drug discovery e Metabolism studies on agrochemicals or pharmaceutical products The above list of typical applications is not exhaustive The traditional market position of CTC Analytics AG has resulted in the PAL System being mainly used as an autosampler device for the techniques of GC gas chromatography and HPLC high pressure liquid chromatography Sample preparation steps such as filtration purification or separation by mean
79. ed trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt User Manual Installation and Operation syste 4 2 3 Trays In general it is not necessary to teach a Tray position The Tray Holder has a teaching position as described above A Tray is assigned to the Tray Holder The numbers values for the relevant items are stored in the firmware object Tray To complete the combination of a Tray Holder and a Tray it is necessary to assign the corresponding Tray Type to the Tray The Tray Type contains the geometric data for the Tray and information such as how many samples are in a row and a column in the Tray A Tray Type defines the pattern and sampling sequence of sample location within a Tray For details see below As described above the Tray is physically placed in the Tray Holder the firmware assigns the Tray to the Tray Holder and the Tray Type to the Tray This is routine procedure as long as no special circumstances need be considered such as customized Trays or Tray Types PAL Firmware version 4 1 X now makes a correction possible if the Tray surface is not exactly horizontal and planar but is inclined in one or more axes At version 4 1 X this is standard and a dialog window pops up the moment the Tray has been checked out at the corners to verify vial positions and heights How to correct a possible inclination is explained below ror oce v M V i J
80. eginning at PAL Firmware version 2 5 x ZSlideOffRetr If magnetic transport is selected the distance can be defined to move the syringe slider up Z direction lifting up the vial by magnetic force Measured in mm YSlideOffDist If magnetic transport is selected the distance can be defined to move the Injection Unit to the side Y direction to slide the vial off Measured in mm Barcode If a Barcode Reader is installed the mode has to be set for AutoFix None No Barcode Reader option requested for this Tray Type 04 PAL is a registered trademark of CTC Analytics AG Switzerland note syste Z Tolerance Z Retract Max Penetr Needle Penetr Spl1 Offset X Spl1 Offset Y Spl1 Offset Z HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation A tolerance window to give a plus minus range expressed in mm where the Injection Unit must expect an object If the value of this item is gt 0 this is a relative detection mode and the sensor from the Injection Unit needle guide is active If the value of this item is set to 0 the sensor of the needle guide is turned off and the syringe slider moves to an absolute value Z axis position as specified in the object The distance to lift up the syringe slider before the Y Z axes assembly moves across the unit can be specified expressed in mm The maximum allowed Needle Penetration can be defined as a safety so that the user c
81. em used to activate specific solenoid for DLW Wash Station Active Used for Active Wash Station in conjunction with the Wash Station Print with PNo APR WASHPUMP B A Revision B Simplified print activation of pumps is controlled by PAL Firmware Requires Object Lists PAL WashActive Rev B Remark Type Active is not used for Fast Wash Station operation and cannot be used for first generation Active Wash Station with print Revision A installed For these cases is the type Pulse required Flow Control The power source to activate a Wash Station is specified For the Fast Active Wash Station and DLW the Event Power Out1 and Power Out2 is used Ndl Cln Path A Path can be assigned to the Wash Station Waste to The Waste position is assigned to the Wash Station Clean Volume A percentage of the syringe maximum volume is defined for the cleaning step Clean Count The counter monitors the number of wash cycles The setting in the method has the same functionality and the value is mirrored to the Extended User Level Max Fill Spd The fill speed for the syringe is defined with this item If the maximum value of the Fill Speed 10 0 mL s is selected the system takes the injection syringe Fill Speed as defined in the method Max Eject Spd The Eject Speed is the speed used to eject the wash solvent during the wash cyde This speed is usually higher than the Fill Speed If the maximum value of the Eject Speed 10 0 mL s is select
82. ere you plan to install the keypad 2 Install the Keypad mounting bracket on either the right or left side of the X axis 3 Connect one end of the white coiled cable Part No SS8J 700 to the Keypad and the other end to the TERMINAL SER3 interface jack on the rear side of the X axis For details see Figures 19 or 20 Do not interchange the Terminal with LAN cable connector 38 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 Place the Keypad terminal onto its mounting bracket 5 2 5 Installing the Power Supply 1 Locate the power supply the DC power cable Part No RS3M 2000 and the AC power cable 2 Setthe power supply switch to the OFF position 3 Connect one end of the DC power cable to the power supply and the other end to the POWER connector at the rear side of the X axis 4 Connect the female end of the AC power cable to the power supply Then connect the male end to an AC power outlet 5 2 4 Installing the LC Injection Valve 1 Locate the blue valve drive It will have one clamp that is identical to other Object clamps see Fig 15 Loosen the clamp as described above Figure 15 Installing the Injection Valve Drive Eg PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation
83. etration Depth value 8 Press Function Key F3 Movto Zero 9 Verify the defined Needle Penetration Depth value by repeating Step 2 and 3 65 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation Figure 46 Needle Penetration in Valve Inlet Port The setting of the Needle Penetration can also be achieved in the Utilities section Path Menu Utilities Injector LCVIv1 Select function key F3 Moveto Inj and activate Needle Penetr Continue as described above After all positions have been set and the Valve Needle Penetration has been adjusted perform a backup of the existing configuration see point 8 below PAL Software Program PAL is a registered trademark of CTC Analytics AG Switzerland 66 HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 7 Interfacing the HTS PAL to Other Devices 7 1 Synchronization and Output Signals Synchronization Signals Sync Signals are inputs that tell the HTS PAL when to wait or proceed with a sample processing step Output Signals Out Signals are sent from the PAL to external devices to indicate status or completion of particular processing steps These signals are classified as Objects Physical Events e g TTL In1 are also Objects and may be assigned to named signals PAL Cycles require tha
84. fter completion of the syringe wash cyde The solvent flows into the wash port without the restriction of the needle be aware of higher solvent consumption 22 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 5 Vial After selecting the specific Vial type the following functions are available Function Key Description F3 Movto Vial The injection unit moves to the selected vial type By selecting the parameter Needle Penetr on the same screen the Wash Station Needle Penetration value can be checked or changed F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed Available Vial types Standard Vial in Standard Wash Station for GC front position Fiber Exp Position to block needle guide Mainly used for SPME application See SPME User Manual Item Description Needle Penetr By selecting the parameter Needle Penetr the Wash Station needle penetration value can be checked and or changed 4 6 Dilutors After selecting the specific Dilutor the following functions are available Function Key Description F1 Prime The Dilutor syringe is primed with solvent after activating the F1 function F2 Chang DSyr Activating F2 moves the plunger of the Dilutor Syringe to standby position to allow easy access to change the syringe F4 HOME The Injection Unit moves to its
85. ger retaining screw Move the plunger slightly out of the plunger holder Pull the syringe adapter out and then carefully upward to remove the syringe adapter with the syringe from the Injection Unit by PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 Injection Valve 6 1 Valve Drives and Valves General Remarks A HTS PAL can be equipped with 1 or 2 LC Injection valves connected and controlled via the Auxiliary Interface AUX1 AUX2 If more than two valves have to be configured or if an auxiliary Interface is occupied by another module such as a Dilutor then the Multi Position or Serial Valve Drives are required The Serial Valve Drives are connected at SER2 interface with the PAL System or interface MODBUS with the PAL xt system The standard LC injection valve is a six port Cheminert valve Valco VICI On customer demand CTC Analytics can also supply other valve types such as Valco W type or valves with various ports 4 port valves with internal loop and 10 port valves The six port valve is connected to the detection system according to the flow path shown below Plumbing diagrams for other available six port injection valves are provided below as well Details are described in the Addendum of PAL User Manual Valve Drives and Valves See also poster Tips and Hints for PAL Systems PAL is
86. gip AjuaJixe jo Sjuauoduo pim Sajduues JO Z pue uas as SuluSeM IS0g pue Bd auuds samjan SA OSL 01 06 a8uu s rl 001 10j paads p h quanos jo Ayso2sia uo spuadap s 1rl opc 0 dn paads jjj smoye zz 38ner amp zz 0010 X JAS jueos jo Ajsoosi uo Spuadap s m oz s sAr OZ xew oj peads iij suw SZZ a8nen Szz 0019 X JAS a8uu s uayu doo jo 20g OZ sdoo ja2 e1 1H 001 5 sdoo 20 1uajuo doo Jo 9609 OZ Adu auunjoa ajdwes pao Buy doo Jered saun 6 PIANO Tri oz azis doo suonipuoo SuIMO O aui 104 ajduiex3 0 NS UD AA t LAS UD AA 0 INS Up ISd t IAS UD Sd sul 005 pa lu isq pa lur aug sits paads palu LAI op palu SOLE pa dnjjng f SYON I sim c pads z Ids up aug 0 TNS UD 34d l INS UD 3d juo 3uinjo ary m 08 3ulnjoA 3iduies m ool 3ONIM S 4 lui 51 IMA fur 51 SI3jauPJeg POLIEN Tvd ap papuatuulo33y poya PAL is a registered trademark of CTC Analytics AG Switzerland Figure 72 HTS PAL Firmware Overview page 4 HTS PAL HTX PAL 6 n 5 gt lt m D I A O c g 7 O O B oc SES rs i e 2E aD E system 4 2 PAL HTS xt System Flow Chart based on PAL Firmware Version 4 1 X Firmware version 4 1 X as shown in the flow chart corresponds to the PAL System The changes from Firmware version 2 5 X as used for the PAL are emphasized in this PAL User Manual note System Aejog pala paads
87. h the PAL System This counter monitors the valve switches and not the number of penetrations into the injection port e g for cleaning the valve an extra penetration Is required It is advisable to monitor this counter in conjunction with the test for the tightness of the Needle Seal as described above e After running routinely a few weeks the warning limit for the Inject Counter gradually becomes more evident and will be a valuable tool for the user in routine work e Path Menu Info Maintenance Inject Limit Inject Counts reflects the actual number of injections 6 2 5 2 Part Numbers for Replacement Needle Seals PNo Description PAL NdlSeal Standard Needle Seal pkg of 10 FEP Tubing transparent Ferrule sst Needle Gauge 22 OD 0 72 mm for Valco VICI sst Valves Cheminert and W type PAL NdlSealP Needle Seal pkg of 10 FEP Tubing transparent Ferrule PEEK Needle Gauge 22 OD 0 72 mm for Valco VICI PEAK Valves Cheminert type PAL NdlSeal 19 Needle Seal pkg of 10 FEP Tubing blue Ferrule sst Needle Gauge 19 OD 1 06 mm for Valco VICI sst Valves Cheminert and W type with bore size 0 75 mm large volume injection application PAL NdlSealR Needle Seal pkg of 10 FEP Tubing transparent Ferrule sst Needle Gauge 22 OD 0 72 mm for Rheodyne Valve 6 port PD 7991 type 64 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYST
88. he power ON and OFF switch is accessible and easy to operate and where it is possible to unplug the AC power cord from the power supply wall outlet in case of emergency PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Other Hazards 3 Fi x 3 To avoid injury during PAL System operation keep your hands away from the syringe A Do not operate the PAL System without the safety shield The safety shield must be installed for safe operation A To avoid injury observe safe laboratory practice when handling solvents changing tubing or operating the PAL System Know the physical and chemical properties of the solvents you use See the Material Safety Data Sheets from the manufacturer of the solvents being used A Use caution when working with any polymer tubing under pressure e Always wear eye protection when near pressurized polymer tubing Donotuse polymer tubing that has been severely stressed or kinked Donotuse polymer tubing in particular no PEEK or Tefzel tubing when using tetrahydrofuran THF dimethylsulfoxid DMSO chlorinated organic solvents concentrated mineral acids such as nitric phosphoric or sulfuric acids or any compounds related to the above A Do not use vials without a sealing cap microtiter or deepwell plates without a plate seal Vapor phase from organic solvents
89. he sample processing Method for this Job 7 Press the Home function key F4 to return to the JOB QUEUE screen 8 To add additional samples to be processed repeat steps 2 7 9 f necessary replace and or clean the syringe liquid versions only Press the Menu key to see the available options for changing F1 Change Syringe and cleaning F2 Clean Syringe To completely remove air bubbles the syringe should be primed manually See Section F Description and Installation point 5 2 Syringe Priming 10 If only one Job is to be processed select the Job with the scroll knob Press Start key In the dialog box Select Job s to Process select one of the following options All Entire Job Queue starting from the top e Selected Job selected with the cursor bar e Resume Continue with the next Job after the one aborted 3 2 Aborting a Job Queue 1 Press STOP 2 Select one of the available options Continue Sample Job or Job Queue 3 Select Continue to resume processing with the current sample 4 Select Sample if there is a problem with only the current sample Processing will resume with the next sample PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 5 Select Job to abort processing all samples in the current Job Processing will resume with the next Job The aborted Job is marked w
90. his utility the selected Tray including the sample vials must be present After pressing Movto 001 the Injection Unit moves to sample position no 1 This procedure can be repeated for the last sample position in the first row and the last sample position F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Tray items may be changed by selecting the particular item Item Description Needle Penetr Needle penetration depth into the sample vial The needle penetration depth for the selected Tray can be changed by entering the desired value Tray Type The Tray Type which is selected for the Tray is shown If the Tray enables the use of different Tray Types it can be changed at this position Tray Offset X If necessary a correction to the ideal X position of Position 1 can be made by using Tray Offset X Tray Offset Y If necessary a correction to the ideal Y position of Position 1 can be made by using Tray Offset Y Tray Offset Z If necessary a correction to the ideal Z position of Position 1 can be made by using Tray Offset Z dxRow Correction of any inclination of a Tray plate in the X axis of a row see Fig 25 for details dyRow Correction of any inclination of a Tray plate in the Y axis of a row see Fig 25 for details dzRow Correction of any inclination of a Tray plate in the Z axis of a row see Fig 25 for details dxCol Correction of any inclination of a Tra
91. ify activation of the sensor Z Limit Actual status of Z axis sensor Move the syringe slider assembly manually up to the zero Z axis point crossing over the limit switch to verify activation of the sensor Aux1 Limit Actual status of Aux limit sensor Aux2 Limit Actual status of Aux2 limit sensor Plg Pos Actual status of Plg Pos Needle Guide Actual status of needle guide sensor Items displayed in italic are available beginning with PAL Firmware Version 4 1 X 1 i Please note that the service test Test Head is executed in the same way independent of the PAL Firmware version However the test evaluation is different between firmware versions 2 3 and version 4 The updated PAL Service Manual Edition 1 1 will include the PAL x System and will explain these differences o0 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation 10 3 Section F3 Setup Objects The same as is described above applies for the section Objects opened in Extended User Mode New classes are visualized and known classes may contain new items accessible only on the level of the Extended User Mode To open this level use the following path Menu F Setup Object Syringes Vials Tray Types Tray Holders Trays Wash Stations Injectors Valves Objects Syringes Vials Tray
92. ime can be selected between sample pullup and ejection while filling the syringe When the plunger reaches the zero position during the fill strokes the system waits half the Pullup Del time This allows for an air bubble to float away from the needle tip This feature is especially useful for removing any air bubble in the syringe and handling viscous fluids Speed of plunger movement used in all syringe filling operations Speed of plunger movement used in all syringe eject operations except sample injection Speed of plunger movement for sample injection Typically used for Fill Strokes Plunger position during Change Syringe operation The syringe plunger is moved to a position where the syringe can be removed and replaced The value may be changed for different types of syringes 20 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt n SYSTEM User Manual Installation and Operation 4 2 Tray After selecting the particular Tray to be accessed the following functions are available Function Key Description F2 Block NdlG F2 activates Needle Guide blocking The option after activation is Rel NdlG releasing Needle Guide Blocking It may be used to test the functionality of the solenoid that blocks the needle guide F3 Movto nnn This function serves as a quick check to determine if the X Y Z coordinates are defined correctly for the selected Tray To use t
93. int in all cases compared to original plunger referencing and defining the syringe zero point If an error is detected the PAL System stops operation This feature is useful if sample solutions with particles need to be handled or if the syringe type is not adequate for the solution composition or if the syringe plunger is blocked or even bent such an error will be recognized and the system will come to an emergency stop Changing a syringe using the Menu function Chnge Syr moves the plunger up to the position specified by this item For normal use accept the default values The syringe plunger can be initialized referenced at Home or Waste position If samples with a high danger of toxicity are treated it is advisable to use the Waste position for referencing al An Autosampler is usually the master in a total chromatographic system The other components send a Ready signal to enable a start of the Autosampler At the moment of injection a Start signal is sent from the PAL System to the other component s of the entire system With this Inj Signal item the status at which the signal is sent out can be defined The syringe is filled with the defined sample volume PlgUp Start signal at the moment the plunger starts moving down for injection PlgDown Start signal at the moment the plunger reached zero point from syringe recommended for large volume injection but not in combination with Agilent Chemstation Va
94. intenance Item Description PlgStrokeCnt This is a counter for syringe plunger movements The actual number of strokes is displayed If the counter reaches the set limit PlgStrkeLim a warning is displayed at the next start of a job run The system continues but signals the user to verify syringe conditions The counter can be set back to zero to restart There is only one counter for a syringe If syringe types are changed the system continues to count as if it were the same type PlgStrokeLim An upper limit for the syringe plunger strokes can be set Inject Count This counter monitors the number of injections The number of injection valve switches Is a helpful tool for the user to decide on a replacement of parts of the injector system The actual number of injections valve switches is displayed If the counter reaches the set limit Inject Limit a warning IS displayed at the next start of a job run Inject Limit The same counter is used for injector penetrations with GC technique Inject Limit An upper limit for the number of injections can be set Counters for the plunger movement and injector penetrations are available with PAL Firmware version 2 5 X or higher 6 4 Free Objects Free Items In addition to the core software the PAL Firmware contains data for the Firmware Objects There are different classes of Objects such as Syringes Trays Tray Holders etc Each class of Objects contains Items The items
95. ion valve Inject to Injectors 13 Wait for Inject signal Inject Sync Signals 14 Drive needle to penetration depth Inject to Injectors Needle Penetr 15 If SplTrnsfDel 0 then switch Inject to Injectors injection valve to ACTIVE LOAD SpITmsfDel position 16 Wait for Pre Injection Delay Pre Inj Del 17 Dispense syringe contents into Inject Speed sample loop 18 Wait for Post Injection Delay Pst Inj Del 19 Switch Valve to STANDBY INJECT Inject to Injectors position SpITrnsfDel If SplTrnsfDel gt 0 then switch valve back to ACTIVE LOAD after time SplTrnsfDel has expired 20 Activate Injected Signal Injected Out Signals If Pulse Time 0 System InjSignal ValveSw min Pulse Time of 300 ms IS used Can be extended with Pulse Time 21 Start timer Cut Delay 22 Retract needle from injector PAL is a registered trademark of CTC Analytics AG Switzerland 110 system HTS PAL HTX PAL PAL HTS x PAL HTX xt User Manual Installation and Operation No Processing Step Method Object Class Used Object Parameter Value 25 Clean syringe and needle with Pst Cln Slv1 SYRINGE Syringes Fill Speed solvent from Wash1 Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 24 Clean syringe and needle with Pst Cln Slv2 SYRINGE Syringes Fill Speed solvent from Wash2 Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Max Fill Spee
96. ir Volume gt 0 Fill Speed Fill Volume Fill Strokes TRAY Tray Type Needle Penetr Pullup Del 8 Move to injection valve Inject to 9 Wait for Inject signal Inject Sync Signals 10 Drive needle to penetration depth Inject to Injectors Needle Penetr 11 Delay Pre Inj Del 12 Switch valve to Active position 13 Activate Injected signal Injected Out Signals Pulse Time 14 Activate Injectd Signal Injectd Out Signals Pulse Time 15 Dispense syringe contents into Inject Speed sample loop 16 Delay Pst Inj Del 17 Switch valve to Standby position 18 Retract needle from injector 19 Clean syringe and needle with Pst Cln Slv1 SYRINGE Syringes Fill Speed solvent from Wash1 Eject Speed Wash Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 20 Clean syringe and needle with Pst Cln Slv2 SYRINGE Syringes Fill Speed solvent from Wash2 Eject Speed Wash2 Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 21 Rinse valve inlet with solvent from Vlv Cln Slv1 SYRINGE Syringes Fill Speed Wash1 Inject Speed Washi Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 22 Rinse valve inlet with solvent from Vliv Cln Slv2 SYRINGE Syringes Fill Speed Wash2 Inject Speed Washi Wash Stations Needle Penetr Clean Volume Max Fill Speed Max Eject Speed 25 Move to Home position Home Positions PAL is a registered trademark of CTC Analytics AG Switzerland 108 syste
97. ith an X 6 Select Job Queue to abort processing all Jobs The JOB QUEUE screen will be displayed The aborted Job is marked with an X 5 5 Restarting an aborted Job Queue l Press the START key 2 Select the Resume option The Job after the last one marked as aborted will be started 4 Utility Functions Utility functions selectable from the Menu screen provide quick access to checking operations and parameters that may need to be changed These functions are available for the actual Syringe Trays Injectors and the Wash Station They allow access to key functions without having to set up and execute a Method and Job If an item is used in the sample processing cycle the appropriate Utility value will be overwritten by the Method value Menu Utilities Utilities Wash Station Scroll down 2009 05 06 16 07 20 E E E Figure 5 Selecting Utilities Functions PAL is a registered trademark of CTC Analytics AG Switzerland syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 4 1 Syringe The following functions are available by pressing a Function Key Function Key F1 Chang Syr F2 Clean Syr F3 Set Pos F4 HOME Description The syringe is moved to a position in which the syringe assembly can be completely lowered to facilitate removal of the syringe adapter The syringe can then be removed from the adapter and replaced A prompt will be displayed
98. letter e g H stamped on the new or cleaned rotor and orient the letter to the lower half of the valve 5 nsert the new or cleaned rotor Make sure the rotor tab fits into the slot in the valve drive 6 Replace the knurled pre load assembly 7 Tighten the pre load assembly It must end up fully bottomed out 99 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 2 3 Replacing Rheodyne PD7991 Type Valve Rotor To replace and or clean the valve rotor of a Rheodyne PD7991 type valve follow the steps shown in Figure 61 The Rheodyne Valve PD7991 can be maintained in a similar way as the Cheminert valve type Replaang the rotor has to be done carefully as it is possible to insert it incorrectly upside down Below the correct orientation of the Rotor guide pin is illustrated Rotor position view from backside of valve body actuator side Small Groove top Flat side on Bar Shaft Large Groove bottom Figure 65 Rheodyne Valve PD7991 Rotor Position Valve Drive position in Home or Standby position Small align pin top Bar sits in slot bottom right Large align pin bottom Figure 66 Valve Drive Settings for Rheodyne Valve PD7991 100 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual
99. lveSw Start signal sent at the moment the injection valve is switched HPLC technique Prelnj Start signal sent at a time before injection as with a sample preparation device started before a GC or LC system Minus time relative to chromatographic start time 78 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Vial PrePress This item can be activated to avoid a vacuum if a relatively large amount of sample is taken out of a small volume vial If e g a volume of 50 uL is specified for injection the PAL system first takes 50 pL ambient air and injects this volume into the sealed vial causing overpressure in the vial After this step the sample volume can be aspirated without forming a vacuum This functionality is only valid for liquid sampling not for the Headspace technique Starting with PAL Firmware version 4 1 X this item is also active in combination with Cyde Composer LC Inj Standard cyde for HPLC technique Details on method see Appendices point 5 Injection Cycles and point 4 How Chart Firmware Overview LC Cut Cyde for column switching with two valves HPLC technique Both valve drives have to be controlled through AUX interfaces Details on method parameters see Appendices point 4 Flow Chart Firmware Overview GC Inj Standard cyde for GC technique Details on method p
100. ming the syringe 25 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 7 Tools The Object class Tools is available with PAL Firmware version 3 0 X 4 1 X or higher After selecting the specific tool the following functions become available Function Key Description F1 Chk Offs The item Check Offset can be used to verify the offset from the syringe needle tip to the tool tip This item is mainly used for the MALDI tool it is not active for the MHE tool F4 HOME The injection unit moves to its HOME position and the Job Queue Menu is displayed The following tool items may be changed by selecting the particular item Item Description Teach Point The reference point at which to teach the Object Tool MHETool is selectable In the case of the MHETool no extra position is necessary The Parking Station is the fix point Select None ToolOffset X If necessary a correction to the ideal X position of the MHETool can be made by using ToolOffsetXX ToolOffset Y If necessary a correction to the ideal Y position of the MHETool can be made by using ToolOffsetY ToolOffset 7 If necessary a correction to the ideal Z position of the MHETool can be made by using ToolOffsetZ A ToolOffsetZ of 48 0 mm is necessary to position the MHETool on the sample vial 5 Logfile The PAL logfile can
101. ms are available directly as Method parameters The Firmware Objects accessed in the PAL Extended User Mode have more specific items selectable than at the User level Tolerances or ranges such as Maximum Fill Speed can be defined and limited for the user so that this value cannot be exceeded Items such as Prime Volume Waste to Motor Drives or Scale Length are specific items that remain hidden at the User level The Object dass Tools is used to handle another device such as the MHE Tool Multiple Headspace Extraction Tool or MALDI Tool For details see the specific Addendum for the PAL User Manual The various positions used with the PAL System are selectable at this level Positions H ii such as Home Change Syringe or a Path Point reference the Paths for a specified Tray Holder directly such as Agitator or Stack Selecting an item e g Home will provide the function key F1 Check Pos on the screen Activating this function provides the possibility of verifying X Y Z axes values for this particular position A Path defines specific movements for the Injection Unit which represent a repetitive task assigned to a Tray Holder Typical examples are opening and dosing a drawer from a Stack or opening the lid of an Agitator The Path is composed of several steps points which are added up in a sequence Each individual step point can be positioned by teaching X Y Z axes In most cases the ret
102. n be set for the PAL internal dock Day of Month The day can be set for the PAL internal clock Hours The hours can be set for the PAL internal dock Minutes The minutes can be set for the PAL internal dock Seconds The seconds can be set for the PAL internal dock After setting or resetting the date and time use the function key FT Set Time to store 7 5 Objects The various PAL Firmware Object classes can be selected and the functions most used by the user are directly accessible For detailed listing see PAL Firmware overview in the appendices 27 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation F HTS PAL Description and Installation 1 General System Overview Injection Unit Z Axis 9 oY Axis Plunger Holder d Keypad Terminal Syringe and Syringe Adapter M y E y o M FF Safety Guard E Microplate Stack Tray Holder Tray Fast Wash Station I LC Injection Valve Figure 8 HTS PAL Major System Components The standard HTS PAL includes the following hardware 1 X Y axes assembly 1 Injection Unit Z axis 1 Syringe kit for liquid injections 1 Fast Wash Station for two different solvents 1 Keypad terminal 1 Safety Guard 1 Standard standalone supports 215 mm Optional TraySets Tray Holder and Tray and or Stacks of various types and
103. ned to the Tray Holder as specified in this combination Correction for inclination of a Tray in X row direction For details see Section 4 2 5 2 above Correction for inclination of a Tray in Y row direction For details see Section 4 2 5 2 above Correction for inclination of a Tray in Z row direction For details see Section F Description and Installation point 4 2 5 2 above Correction for inclination of a Tray in X column direction For details see Section F Description and Installation point 4 2 5 2 above Correction for inclination of a Tray in Y column direction For details see Section F Description and Installation point 4 2 5 2 above Correction for inclination of a Tray in Z column direction For details see Section F Description and Installation point 4 2 5 2 above Items displayed in italic are available beginning with PAL Firmware Version 4 1 X 0 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation syste 10 3 3 Wash Stations The following object Wash Station items may be changed by selecting the particular item First select the desired Wash Station such as Wash1 Item Description Position X X axis position for Wash Station Position Y Y axis position for Wash Station Position Z axis position for Wash Station Z Tolerance A tolerance window for a plus minus
104. nge detection can be turned off in cases of sensor failure or if a spec syringe with unknown ID is installed Turning OFF the syringe identification sensor automatically displays a dialog box enabling the selection of syringe identification manually This item provides the choice of referencing all axes at the start of the system or suppressing the referencing It is advisable to use this function actively to detect a possible loss of steps This function allows turning off the automatic recovery of the PAL system after detection of a collision loss of steps of a stepper motor Stop XY Error 2 ON Automated recovery turned OFF Stop XY Error 2 OFF Automated recovery turned ON In PAL Firmware version 2 3 X the new feature that recovers automatically after detecting a collision has been implemented Firmware version 2 6 2 provides the possibility of disabling automated recovery If recovery is turned OFF the PAL system always checks its position before moving to the injection port for injection This can lead to an extra move crossing over the X Y axes sensors if the injection port is at the opposite end of the X axis relative to the sample location zero X axis position in between sample location and injection port For critical application fields e g clinical analyses it is advisable to turn off the automated recovery Stop XY Error ON The plunger travel path is monitored to ensure that the plunger tip reaches the syringe zero po
105. not be read out directly on the terminal display However the function key F3 does allow a print out A serial printer has to be connected to port SER2 for the PAL System and to the port SERT for PAL Xt System If a serial printer is not available use a serial parallel converter 24 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation 6 Info Functions Info functions selectable from the Menu screen provide quick access to information to be viewed These info functions are available for the Hardware Software Maintenance Free Objects and Free Items All items with the exception of Maintenance are read only Menu Info Scroll down 2009 05 06 16 07 20 Figure 6 Selecting Info Functions 6 1 Hardware Item Description CPU SNo The serial number SNo of the PCB APR CPU is displayed CPU ID Version number of the PCB APR CPU MOTIO ID Version number of the PCB APR CPU 6 2 Software Item Description PAL Firmware Firmware version of PAL System Head Firmware Firmware version of PAL Injection Unit Terminal FW Firmware version of PAL Terminal Altera Firmware Firmware version of the Altera component 25 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 3 Ma
106. only fuses of the type and current rating specified Do not use repaired fuses and do not short circuit the fuse holder The supplied power cord must be inserted into a power outlet with a protective earth contact ground When using an extension cord make sure that the cord also has an earth contact PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX xt PA SYSTEM User Manual Installation and Operation Do not change the external or internal grounding connections Tampering with or disconnecting these connections could endanger you and or damage the PAL System The instrument is properly grounded in accordance with these regulations when shipped You do not need to make any changes to the electrical connections or the instrument s chassis to ensure safe operation A The combination of a PAL System with a LC MS System does require the safety measure as described by the LC MS System manufacturer Detailed instructions for the safety grounding on the LC MS system are outlined in the corresponding operating installation manual CTC Analytics recommends using a grounding cable connected on one side at the Injection Valve Loop or any other suitable direct metallic contact and on the other side at an appropriate ground point on the LC MS System This supplementary grounding measure will support the safety strategy of the LC MS System manufacturer A Do not turn the instrument
107. or measurement One meter from the equipment in the direction of maximum sound pressure level According to UL 610107A 1 1 edition clause 12 5 Limit lt 85 dBA dBA A weighted sound pressure level 5 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 1 7 Hardware and Software Requirements 1 7 1 Hardware Requirements In order to operate a PAL System the electronic control board must be compatible with the corresponding PAL System PAL System operated with PAL Firmware version 2 X X or 3 0 X either the combination of APR CPU and APR MOTIO or the APR Control boards can be used PAL xt systems operating with PAL Firmware version 4 1 X or higher APR Control board must be installed A PAL System can be upgraded to a PAL xfSystem for details contact your CTC Analytics representative For specific PAL hardware Modules a minimum PAL Firmware version Is required For example a PAL Dilutor Option requires the PAL Firmware version 2 3 X or higher and a PAL DLW Option requires the PAL Firmware version 4 1 X or higher Details are given either in the corresponding Addendum to PAL User Manual or in the Addendum to PAL User Manual for PAL Object Manager Software 1 7 2 Software Requirements The various PAL Software programs such as PAL Loader or PAL Object Manager
108. pal paads i4 Aejaq dnyind Z SHO 00 awNjoA Wld A ISHO 00 JAS 10 njiq X FSYO O0 sod IN q JAS ulog yea 38uu S OOLIGIWW jh ona 0l 6007 UNF XQ Seu H UOISIN WA SAS TWd enpiupur pea 104 uoneinaijuoo asempiey au uo spuadap Moke aut MAINO BY ur UMOYS SP palqo Asana apnpul JOU SBOP SIEMYOS piepuers ayy axueuajule N ojuj NUayy Jajuno pis aA PA pue BYONS JaBUN g 3 0N uui puojuy Aejag 1m9 AAPA IND ZNS UD AYA LAS UD AA ZNS UD NA I 2M0H CNS UD Isd IAS UD NA a INS UD 38d ZNS UD Sd ne pasues ds LAIS UD 15d pa fusd eq lul isd 90 ORS oq lul aud aq lu aid obo pads pa pads Dol ZB o palu o palu 01 10 DAE pa dnind Ba dnind e up By pdi pedel ZABIJ SOg 3 I TS pee ASD Aupa amh OM ARYO paads jl INS UD alg IS UD ad aan NADI sit eh eg dnjng auinjoA Jv atunoA IY Jjouad 9 paaN jjouag APN 1jguag a paaN sas IH SURG SUA d 3Uun oA IH JONIYAS JONIYAS mua APN sem Ax L Uer qi env 1D 1D CPA uowsusew opu Mj PE ONY Le U BELT M IAJ AQ 9JeMUll4 JX S1H nasas Jud Figure 73 PAL HTS xt Firmware Overview Page 1 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL ec Ini 5 gt lt q I a O A _ MW O Sc gees LE zt I e Tat aD S amp 01 3 S AA 3uunjoA SW 3uinjoA aqn Aejag pal paads p paads Il4 pa dnijng pds pala xe pds i4 Xew paads
109. r Manual Installation and Operation Figure 11 Connecting the Injection Unit Ribbon Cable 3 Hold the Injection Unit in place against the Y ax s Make sure the two locating pins on the Y ax s fit into the two guide pin holes on the Injection Unit 4 Place one of the screws onto the end of the supplied Torx driver Slide the clear plastic cover on the Injection Unit all the way to the top Locate the three large holes in the black anodized frame attached to the Z axis inside the Injection Unit Slide the frame upwards until the top hole is centered on the top threaded hole at the end of the Y axis Insert and securely tighten the Torx screw A see Fig 12 Figure 12 Inserting the Injection Unit Mounting Torx Screws 5 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 5 Install the two remaining Torx screws B C in the left and right mounting holes respectively It may be necessary to move the elastic cord slightly to the left to insert the Torx screw C into the right hand hole Figure 13 HTS PAL with Injection Unit and Standalone Supports 5 2 2 Installing the Keypad Terminal Figure 14 Installing the Keypad Terminal 1 Install the safety shield on the left and right sides to the outside of the X axis Use the provided longer thumbscrew on the side wh
110. r biological samples First wash is always an aqueous solution followed by organic solvents Define Object s correctly See section F Describing and Installing point 4 1 Redefine Z value for the affected Object See section F Describing and Installing point 4 1 Check position and insert if necessary vial deepwell or microtiter plate Redefine value for the affected object See section F point 4 1 Switch OFF PAL and check connection between PAL and Valve Drive Defective LCVlv Connection cable Change connection cable MOTIO Board defective Replace MOTIO or Control x Board 90 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation H Replacing Parts Safety Warning Always disconnect the power cord s from the power supply or from the various power supplies if optional devices are installed before attempting any type of maintenance Capacitors inside the instrument may still remain charged even if the instrument Is turned off To avoid damaging electrical parts do not disconnect an electrical assembly while power is applied to the PAL system Once the power is turned Off wait approximately 30 seconds before you disconnect an assembly 1 MOTIO Board PNo APR Motio 1 Follow the sequence shown in Figure 56 to detach and release the MOTIO board from
111. rchangeable 61 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 6 2 4 Rheodyne 6 Port Injection Valve PD718 313 Sample loop ff I 2 eJ Carrier mobile phase Figure 43 Flow Path LC Injection Valve Rheodyne PD718 313 The Rheodyne Injection Valve PD718 313 is a SupraLife 2 position 6 port Rapid Replacement Pod equipped with a vertical inlet port delivered with CTC Needle Guide and Needle Seal Valve pod diameter 52 mm Valve Stator Coated stainless stator Rotor RPC 13 Polyimide based rotor seal Bore Size Vertical Port 0 25 mm 0 010 Ports 2 to 6 0 20 mm 0 008 Volume for Vertical Port 0 75 uL Ports and Engraving Ports 2 to 6 0 07 uL Engraving 0 05 uL standard engraving Valve Volume Full Loop 0 87 uL Partial Loop 0 82 uL Connections 10 32 male threaded fittings 1 16 tubing OD Port Nut Ferrules Swagelok Nut and Front Back Ferrule Maximum Pressure 1200 bar 18000 psi 120 MPa Standard Loop 2 uL Loop ID 0 13 mm 0 005 Sample loops of the different valve types are not interchangeable The Agilent specific Rheodyne Valve with Agilent PNo 5067 412 Is connected in the same way to the HPLC system as shown in Figure 43 62 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA
112. result of normal use or manufacturing defect What is NOT covered by this warranty This warranty does not cover defects or failures resulting from damage caused by accident misuse or abuse such as Improper or unauthorized service or repair Failure to follow the operating instructions provided by CTC Analytics AG Improper or insufficient ventilation Force Majeure No liability for events beyond its reasonable control including but not limited to fire storm flood earthquake explosions riots strikes labor disputes transportation embargoes or other contingencies beyond the control of CTC Analytics AG i e act of God or Force Majeure Exposure to corrosive compounds The warranty does not cover parts exposed directly to liquids such as valves valve rotors or other valve components tubing or syringes or any other parts considered consumables and wear parts What Is the Period of Coverage This warranty remains in effect for a period of one year from the date of installation What will CTC Analytics do to correct Problems CTC Analytics AG is represented by an authorized distributing or OEM partner In a respective country These partners must be contacted to request technical assistance by phone to diagnose the nature and probable cause of any malfunction If we determine that the problem is due to a defect in the instrument or a module any replacement module s that has have been determined to be
113. rk of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 8 2 PAL Object Manager Software The PAL Object Manager software is used to load a new PAL Firmware Object to a system after a new module hardware module has been added to the PAL System The PAL Object Manager software is the software used to handle the so called PAL Object List The software version 2 2 1 or higher has been developed for the PAL xt System that in addition to serial communication also permits LAN communication TCP IP The software is backwards compatible and can be used for all PAL Firmware versions The PAL Object Lists have to match the corresponding PAL Firmware version as shown in the list below e PAL System Firmware Versions 2 X X PAL Object Lists Rev K or higher e PAL System Firmware Version 3 0 X PAL Object Lists Rev K or higher See specific Object Lists for this FW Version 5 0 X e PAL XfSystem Firmware Version 4 1 X or higher PAL xt Object Lists Rev B or higher The two Object Lists for the PAL and PAL x Systems can be loaded with the same PAL Object Manager software This simplifies the handling in cases where the PAL and PAL Xf Systems are in use within the same working environment Detailed information is available in the Addendum to PAL User Manual PAL Object Manager Software 70 PAL is a registered trademark of CTC Analytics AG
114. romatography Check tubing internal diameters for connection tubings and injection loop See recommendations as given with poster Tips amp Hints for HPLC Technique CTC Anayltics Connection tubing s not square cut or wrong type of nuts ferrules installed See recommendations as given with poster Tips amp Hints for HPLC Technique CTC Analytics Any parameter like flow rate composition of mobile phase or wash solvent column selection diameter pore size etc can contribute to BAD PEAK SHAPE Ched troubleshooting guide from the HPLC manufacturer or any other source Increase Pst Inj Slv1 Slv2 values in method Use PreCInSlv1 Slv2 and PreCInSpl Inappropriate wash solvent s Use appropriate wash solvent s Waste tubing ID at injection valve is too small causing waste liquid to be pulled back by capillary action into rotor groove Damaged or grooved valve rotor Valve needle seal leaks Inappropriate wash solvent composition Object s not defined correctly Incorrect Z value for the affected Object e g LCVIv1 THldr1 or CStk1 01 Missing sample vial Deepwell or Microtiter plate Incorrect Z value for the affected object Injection valve not connected Replace the waste tubing with larger ID tubing Replace valve rotor see section H point 4 Change valve needle seal see section F point 6 2 5 Use appropriate wash solvent Observe also the order of use fo
115. rotor and a the special needle guide Stator material Stainless steel 516 Rotor material Valcon H inert mix polymer Port size 1 16 inch Fittings Valco 1 16 inch Ferrules and Nuts Actuator PAL Valve Drive Micro Electric Actuator Loop size 20 uL shipped Bore size 0 40 mm Port to port volume 155 nL for all ports 390 nL for engraving Sample loops of the different valve types are not interchangeable 60 PAL is a registered trademark of CTC Analytics AG Switzerland note system HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 6 2 3 Rheodyne 6 Port Injection Valve PD7991 Carrier mobile phase Sample in Vent waste Sample loop Figure 42 Flow Path LC Injection Valve Rheodyne PD7991 The Rheodyne PD7991 six port two way switching valve is delivered with a CTC Needle Guide and Needle Seal Valve pod diameter 27 mm Rheodyne Valve Stator material Rotor material Bore size Port size Port ferrules Needle seal ferrule Nuts Loop sizes Maximum pressure Port to port volume Flat Plate Rotary Valve SPC 4 special plating on Titanium RPC 10 proprietary PEEK blend 0 20 mm 0 008 Vertical Port 0 25 0 010 1 16 inch Swagelok Front and Back Ferrules Rheodyne Rheodyne nuts 5 10 20 50 100 uL 1034 bar 15 000 psi 294 nL for vertical port 195 nL for standard ports Sample loops of the different valve types are not inte
116. s Objects are data structures describing the properties of physical modules Certain modules e g a Stack require several objects Module PAL hardware module either part of a standard PAL configuration e g COMBI PAL HTS PAL or an optional addition e g Cooled Stack MALDI Tool Dilutor The term Module is intentionally used to differentiate from Object which is reserved for the PAL Firmware Object 106 PAL is a registered trademark of CTC Analytics AG Switzerland syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation 2 Naming Convention This section recommends the standard naming convention for HTS PAL Trays Tray Types and Tray holders Following these conventions will allow the HTS PAL to be pre configured for certain applications will simplify software backups and application development and will improve technical support and training Tray Type Tray Description VT200 Vial Tray 200 positions 10 x 20 For 7 mm micro vials 1 mL VT98 Vial Trays 98 positions 7 x 14 For 12 mm vials 2 mL VT78 Vial Tray 78 positions 6 x 12 For 7 mm micro vials 1 mL opposite side of 98 positions Tray VI54 Vial Tray 54 positions 6 x 9 For 12 mm vials 2 mL VI21 Vial Tray 21 Positions 7 x 14 For 12 mm vials 2 mL opposite side of 32 positions Tray VT22 10 Vial Tray 32 positions 4 x 8 For 25 mm headspace vials 10 mL VT22 20 Vial Tray 32 positions 4 x 8 For 2
117. s are repeated for each sample within a Job Cycles are designed for specific applications Method A Method defines how the samples are processed The elements of a Method are a Cycle a Syringe and a Parameter List Methods have names with up to eight characters and can be edited copied and deleted Method Parameters Method Parameters are associated with the Cycle operations User assigned Parameter values define how a processing operation is performed A zero Parameter value will disable a Cycle operation Cycle Parameters are application specific Tray Holder A Tray Holder can hold one or more trays Each Tray Holder has a reference position X Y Z coordinates that defines its location 104 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX x n SYSTEM User Manual Installation and Operation Tray A Tray holds multiple samples Trays are defined by designating the Tray Type see below and the Tray Holder Tray names are used to Identify the sample source within a PAL Job Tray Type A Tray Type defines the pattern and sampling sequence of sample locations within a Tray Stack A Stack is a particular type of Tray Holder that is designed to hold micro plates A six drawer Stack holds 12 standard microplates two in each drawer A three drawer Stack holds six deep well micro plates two in each drawer PAL Object Manager Software to load a PAL Object List
118. s for PAL and x System 6 Intended Use for PAL System 6 1 Introduction 6 2 Intended Use 6 5 Disclaimer and Restrictions on Use 7 PAL Accessories PAL is a registered trademark of CTC Analytics AG Switzerland PAL Firmware Version 117 121 125 125 125 125 126 syste HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation C List of Figures Figure 1 PAL Control Terminal and Conventions Figure 2 Accessing a Method Screen Figure 5 Verifying Tray with corresponding Tray Type Figure 4 Example of Job Queue Screen Figure 5 Selecting Utilities Functions Figure 6 Selecting Info Functions Figure 7 Selecting Setup Functions Figure 8 HTS PAL Major System Components Figure 9 Attachment of Mounting Claws Figure 10 Attaching the PAL Injection Unit Figure 11 Connecting the Injection Unit Ribbon Cable Figure 12 Inserting the Injection Unit Mounting Torx Screws Figure 13 HTS PAL with Injection Unit and Standalone Supports Figure 14 Installing the Keypad Terminal Figure 15 Installing the Injection Valve Drive Figure 16 Installing the Fast Wash Station Figure 17 Fast Wash Station Assembly Figure 18 Electrical Connection Fast Wash Station Figure 19 Installing a Microplate Stack with 3 or 6 Drawers Figure 20 Installing a Tray Holder Figure 21 Electrical Connections for HTS PAL System Figure 22 Electrical Connections for PAL HTS x Sy
119. s of solid phase extraction SPE derivatization etc are possible in combination with chromatographic or other techniques Other techniques or areas of application are also possible XYZ robotic systems are typically used where vial sample transport liquid handling or sample preparation need to be automated Known fields of application include tablet dissolution and viscosimetry The users will to a large extent themselves determine which substances compounds or liquids the system will come into contact with 6 3 Disclaimer and Restrictions on Use The PAL System boundary represents a significant limitation of the manufacturer s responsibility Submitting and placing samples vials well plates or other sample holding devices inserting syringes selecting reagents and wash solvents are together with any other preparative tasks solely the responsibility of the user Any possible sample mix up or omission of an item cannot be detected or prevented by the PAL System The PAL or PAL xt System boundary is defined when the sample is placed in the system and by the final prepared and primed system The use of the PAL and PAL xt Systems for any invasive technique on human beings or animals is explicitly excluded In addition any application in an environment where explosion protection is required Is specifically prohibited for the PAL and PAL xt Systems 125 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX
120. s of correction Details for teaching are given below f 008 1001 Jos o jJ Figure 25 Inclined Tray Corrections for X Y Z Axes Note that these functions are not available for the HTS PAL if operated solely on PAL Firmware version 2 4 X or higher but neither with firmware version 3 XX nor with version 4 X X PAL is a registered trademark of CTC Analytics AG Switzerland 48 HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 2 Description of Object Positions 4 2 1 Tray Holder e g THldr1 Figure 26 Tray Holder Reference Position For a Tray Holder the Reference Position is a hole in the base plate of the Holder The lower needle guide should be centered in the hole with the bottom of the needle guide flush with the bottom of the base plate Figure 26 shows the example of a Tray Holder for a single Tray e g THldr1 for Tray 1 with Tray Type VT98 and a Tray Holder for four Trays e g Tray 1 with Tray Type VT54 DW96 or MT96 etc 4 2 2 Tray Holders Stack or Stack Cooler with 5 or 6 Drawers Figure 27 Stack Reference Position The Reference Position for a Stack or Stack Cooler is identical The reference position in the case of a 3 Drawer Stack is the hole in the upper drawer and in a 6 Drawer Stack it is in the second drawer from top same height level as shown in Figure 27 49 PAL is a register
121. ser Manual Installation and Operation A Safety Information General Considerations A The PAL System User Manual and the corresponding Addendum for a specific module must be consulted by the user under all circumstances before a unit is put in use Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment The user shall be made aware that if the equipment Is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired When using the PAL System follow the generally accepted procedures for quality control and methods development When using the PAL System in the field of chromatographic analysis if a change in the retention of a particular compound in the resolution between two compounds or in peak shape is observed immediately determine the reason for the changes Do not rely on the separation results until you determine the cause of a change Electrical Hazards A Every analytical instrument has specific hazards so be sure to read and comply with the following precautions They will help ensure the safe long term use of your PAL System The installation category over voltage category for this instrument is Level II The Level Il category pertains to equipment that receives its electrical power from the local level such as an electrical wall outlet A Use
122. service or repair task on the instrument must be carried out by CTC Analytics authorized personnel only Normal Responsibilities of the Buyer The user must report any problem with the equipment to the responsible representative of CTC Analytics AG and assist the technical support representative in diagnosing and attempting to resolve the malfunction of the instrument It is expected that the user will cooperate and will install any replacement parts modules that have been sent and will return any exchanges or unused replacement parts to the responsible representative of CTC Analytics AG at previously agreed conditions How does the LAW of various Countries or States relate to this Warranty This warranty gives you specific legal rights and you may also have other rights which vary from country to country or from state to state How does this Warranty relate to Warranty Statements of various Distributing or OEM Partners This warranty statement from CTC Analytics AG gives the user specific legal rights If the warranty statement from your purchaser distributor or OEM is broader the added value of that warranty will be valid for the user This however is not the responsibility of CTC Analytics AG but solely that of the particular distributor or OEM partner 54 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation
123. stem Figure 23 Object Reference Position Figure 24 Menu Screen Object Tray Holder Figure 25 Inclined Tray Corrections for X Y Z Axes Figure 26 Tray Holder Reference Position Figure 27 Stack Reference Position Figure 28 Demonstrating a possible Inclination of Tray in X Y Z Axes Figure 29 Tray Type VT 32 Figure 50 Tray Type DW96 Figure 51 Utilities Function Trays Figure 52 Adjusting Offset X Y Z for Tray Position 001 Figure 33 Adjusting Row Inclination at Second Tray Corner Position Figure 34 Adjusting Column Inclination at Third Tray Corner Position Figure 35 Wash Station Wash1 Wash2 Reference Point Figure 36 LC Valve and Waste Reference Position Figure 57 Positioning Z Axis Needle Guide on Valve Needle Guide Figure 38 Syringe and Syringe Adapter for Liquid Injections Figure 59 Installing and Removing a Syringe for Liquid Injections Figure 40 Flow Path LC Injection Valve Cheminert Type Figure 41 Flow Path LC Injection Valve W Type Figure 42 How Path LC Injection Valve Rheodyne PD7991 Figure 43 Flow Path LC Injection Valve Rheodyne PD718 313 Figure 44 Valve Needle Guide and Valve Needle Seal Figure 45 Menu Screen Valve Needle Penetration Depth Figure 46 Needle Penetration in Valve Inlet Port Figure 47 COM Port Settings Figure 48 Reading the MAC Address Figure 49 PAL xf Communication Settings Figure 50 Setting Communication Mode PAL is a registered
124. t certain signals such as Start Inject and Injected be defined See the next table below for the PAL Events and signal assignments associated with the standard PAL LC Inj and LC Cut cydes Certain types of integration of the PAL system into data handling or control software such as ChemStation Xcalibur Galaxie or Chromeleon accomplish synchronization of the Ready Start signal directly via software control RS232 LAN A synchronization cable is not required and the Sync Signal Start must be set to Immediat Menu Setup Objects Out Signals Injected Destination SW Outl To assign a physical Event e g SW Out 1 to an existing signal Object Injected complete the following menu selections PAL Systems are shipped with all Cycle events pre defined as shown in the table below If a different physical signal needs to be used then it will be necessary to assign a new Event to the Object signal 67 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation If a signal is to be ignored then its corresponding Event must be set to Immediat Standard Assignment for HTS Description Default INTERFACE PAL Injection Cycles Events 1 Pin Start JobQueue Sync Signal Start a Job Queue Job Immediat Prerequisite is a defined Job with a method assigned Useful for automated unattended timed
125. th PAL Firmware version 4 1 3 Items displayed in italic are available beginning at PAL Firmware Version 4 1 X Items displayed in bold are explained in detail below 05 PAL is a registered trademark of CTC Analytics AG Switzerland note HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation 10 3 1 Tray Type The following object Tray Type Items may be changed by selecting the particular item First select the desired Tray Type such as VT32 20 The definition of Row and Column is given above in Section F Description and Installation point 4 2 3 1 By reversing the first six entries the order of injection can be changed Example Tray Type VT32 20 has a Row Length Y and a Col Length X defined Reversing to Row Length X and Col Length Y would start with sampling from left to right as used e g with Tray Type DW96 The values of the items Spl Per Row and Spl Per Col must also be reversed Item Description Row Length X Defines the Row length in the X direction from the center of the first to the center of the last vial position measured in mm Row Length Y Defines the Row length in the Y direction from the center of the first to the center of the last vial position measured in mm Col Length X Defines the Column length in the X direction from the center of the first to the center of the last vial position measured in mm Col Leng
126. th Y Defines the Column length in the Y direction from the center of the first to the center of the last vial position measured in mm Spl Per Row Number of samples per Row Spl Per Col Number of samples per Column Pattern The pattern of the Row or Column arrangement has to be defined Regular square arrangement standard Stagerd Rows are arranged in staggered pattern Offset of second Row is shifted by 50 of hole pattern Staggrd Rows are arranged in staggered pattern Offset of second Row Is shifted by 50 of hole pattern See Graphics below TrayTypeGroup Software protection so that not every Tray Type can be placed on any Tray Holder Example VT32 20 cannot be fit into a Stack Plate Thickn Thickness of Tray plate bottom not the total height of the Tray Measured in mm Vial Height Total height of the vial including cap to seal if applicable Measured in mm Remark The sum of the plate thickness and vial height adds up to the total height This is the point where the Injection Unit expects an object Vial Trnsprt The mode to transport a vial is defined by None No transport required Example liquid sampling Magnet Magnetic Transport Requires magnetic vial caps The Injection Unit moves to the side to slide the vial off when moved away Needle Device is transported with the syringe needle inserted Example special vials where a magnetic cap cannot be crimped e g Vacutainer Remark This item became active b
127. to an instrument if a Module hardware module has been added to the PAL System In a special mode Object Manager can also be used to create and maintain Object Lists PAL Object List If a PAL Module hardware is added to an instrument several Objects have to be loaded into the firmware These Objects are collected in an Object List and stored in a file with the extension pol Object Lists are delivered together with Object Manager Software and are grouped into folders for the different kind of Modules e g Syringes Tray Holders Valve Drives The name of an Object List starts with the Module part number with variants added e g first or second Stack The name of the root folder includes the revision which is dependent on the firmware version e g Object Lists Rev K for firmware 2 X and 3 X Object Class Each Object belongs to an Object Class e g Syringes Trays Injectors The Object Class defines the Items of an Object Object Item An Object contains several Items which can be numerical values with a physical unit e g X Y Z Position Penetration Syringe Scale Length Syringe Volume or references to other objects Note that the term Parameter is reserved for ATOM Parameter PAL Firmware commands to be used for a PAL Cyde or Macro 105 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation Object
128. to sample vial see section F point 6 2 Sample volume too low The valve needle guide and or needle seal are not properly installed The valve ports are not plumbed correctly to the pump and or detection system Wrong valve type specified The valve rotor is reversed 180 out of alignment Syringe OD too small Valve needle seal leaks the injection valve is not set correctly The syringe Plunger speed is too high resulting in excessive pressure in inlet Air bubbles below Syringe plunger Air bubbles may remain below the plunger after the first pull up If the plunger is moved up and down several times these air bubbles are worked out Dirty syringe Increase sample volume Check valve needle guide and seal see section F point 6 2 5 Check plumbing connections see section F point 6 2 Check valve type by selecting path Menu setup Objects Injectors LCVIv1 valve Remove the rotor and reinstall in the correct position In case of a Cheminert valve check for marking points In case of a W type valve look for a letter e g H stamped on the rotor The letter must be on the lower half of the rotor See section F point 6 2 Check the syringe needle for correct Gauge Gauge 22 OD 0 72 mm Gauge 19 OD 1 06 mm Change valve needle seal see section F point 6 2 5 The needle penetration depth for Adjust the Injection Valve Needle Penetration see section F point 6 2
129. to specify the new syringe The syringe must be installed before pressing Enter See Section F Description and Installation point 5 Syringes This Function is used to clean or prime the syringe prior to use After selecting F2 either Wash1 or Wash can be selected Set Pos is used to define the Chang Syr position The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Syringe items may be changed by selecting the particular item Item Actual ID Fill Volume Fill Strokes Pullup Del Fill Speed Eject Speed Inject Speed Plunger Chnge Pos Description Indicates the identification number ID of the currently inserted syringe If the syringe detection system Is set to manual the message Syringe No syringe is displayed This parameter serves to control the filling of the syringe It can occur that air bubbles remain below the plunger after the first pull up If the plunger is moved up and down several times see Fill Strokes these air bubbles are worked out With this operation the syringe can be completely filled even when using very small sample volumes Number of fill strokes All fill strokes except the last one use the selected fill volume If the selected sample volume is greater than the fill volume the sample volume is used for all fill strokes If zero is selected the plunger is pulled up only once using the sample volume value By using this item a delay t
130. trademark of CTC Analytics AG Switzerland system HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation List of Figures contd Figure 51 Figure 52 Figure 53 Figure 54 Figure 55 Figure 56 Figure 57 Figure 58 Figure 59 Figure 60 Figure 61 Figure 62 Figure 63 Figure 64 Figure 65 Figure 66 Figure 67 Figure 68 Figure 69 Figure 70 Figure 71 Figure 72 Figure 73 Figure 74 Figure 75 Figure 76 Selecting Setup in Extended User Mode Selecting Objects in Extended User Mode Pattern Tray Type Regular Pattern Tray Type Staggered Pattern Tray Type Staggered Replacing MOTIO Board Replacing CPU Board Replacing Control xt Board Replacing the Injection Valve Cheminert Type Replacing the Injection Valve W Type Replacing the Injection Valve Rheodyne PD 7991 Type Replacing the Injection Valve Rheodyne PD719 313 Rotor Replacement of Cheminert Type Valve Rotor Replacement of W Type Valve Rheodyne Valve PD7991 Rotor Position Valve Drive Settings for Rheodyne Valve PD7991 Valve Position Standby and Active PD718 313 Actuator Position Standby and Active PD718 313 HTS PAL Firmware Overview page 1 HTS PAL Firmware Overview page 2 HTS PAL Firmware Overview Page 3 HTS PAL Firmware Overview Page 4 PAL HTS xt Firmware Overview Page 1 PAL HTS xt Firmware Overview Page 2 PAL HTS xt Firmware Overview Page 3 PAL HTS xf Firmware O
131. uly uiSua aeos JAS 10jnjiQ BUL syste ae INO NA Jj9U3g a paaN 7 UOlSOd A UOnISOd X uonisod NAD siopeluj I M0 4 90 DRSI 01 10 LIS USE 9025 pds pala xew O 10 OfIS pds II4 Xe CL DS Ijauag 3 paaN 01 LORS 7 uonisog K UONISOd ae X UOISOg adAy e1 uem Ue suonens usew SMey Spo 6007 aun XQ aeu WOISINaY WAISAS T d Enpiupur yea JO uoneinaijuo asempsey au uo spuadap Moel aut WawWaAo at ur UMOYS se palqo Alana apnpul JOU SBOP IILMYOS piepuels aut 9 0N ors C ao DQ o C bes C a 7 uonisog A UOnISOd X uonisog UPIHL sep Ae SP spelqo JX S1H 3155 Ve Spuo as sanu SJnOH uow jo Aeg Figure 74 PAL HTS xt Firmware Overview Page 2 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL puepazyas ny snApuy 34 JO xieursper padi si qud 3jElS enpy 3 E S JADY ano MS 3jels enpy LIS ADY LNO MS 3jels enpy aui aunogeq IBS APY cu oido ayes jenpy aw aounoqaq LIS anpy Installation and Operation PAL HTS x PAL HTX xt User Manual ales jenpy IPIS AAY 1InO 1LL BPS jenpy RAS AMY ano PS jenpy 3jPls IAY Dno 1LL ays enpy aui aounoqaq aqeys INDY nn nauag 00 aned EUFL d Z SHO oo C ANAN E pnoy u A J8SJO 001 mur Bare aS og pe Sog Bune uomsog UO PS jenpy eppelui CS d Sayed 3yels jenpy uoisod WO awl aounoqag aul
132. urn path is in the reverse order of the starting path Example AgiOpnL and AgiClsL If one setting of a point is changed this change will be valid for the return path as well The various synchronization signals are described above in Section F Description and Installation point 6 1 At this level no other functionality as shown at the user level is provided The various Out Signals are described above in Section F Description and Installation point 6 1 At this level no other functionality as shown at the user level Is provided The various synchronization signals are described above in Section F Description and Installation point 6 1 At this level no other functionality as shown at the user level is provided Three events are newly available at this extended user level Pwr Out1 Pwr Out2 and FlushVlv The main purpose of these three power out signals is to activate a solenoid such as for the Wash Station or the Gas Flush Valve At this level the functionality of the signal can be tested Serial valves or Multiposition Valve Drives are powered and controlled by the serial RS232 control and not through the AUX interface With firmware version 4 1 X a Mode is now selectable The mode refers to the setting of the Valve Drive whether the current for the motor is set to High Speed or High Torque For more details see Addendum to PAL User Manual Valve and Valve Drive The Out Expansion
133. us and the particular function being accessed by the operator All menu screens have the same basic format The menu title is displayed at the top of the screen A list of items is displayed below the title The date and time or status are shown in the highlighted area above the Function key labels on the bottom of the screen PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation Menu Methods LC 100 Scroll down Figure 2 Accessing a Method Screen 1 2 Function Keys Options for a particular menu are assigned to the corresponding function keys F1 F2 F3 and F4 directly below each function key label Pressing the function key labeled Home will always return to the Job Queue menu 1 5 ESCape and STOP Keys Press the ESCape key to return to the previous menu Press the STOP key to abort the current Cycle Job or Job Queue 1 4 Scroll Knob and ENTER Button Rotate the outer knob to scroll through items in a menu list To select a highlighted item press the central knob ENTER button Then use the outer knob to scroll through available options for that item or to change a numeric value Then press the inner knob again to ENTER the displayed option The inner knob is also used for other operations that require an ENTER operation to continue or complete an operation PAL is a registered trademark of CTC Analyti
134. used Path Speed Y axis motor speed in conjunction with a Path example Opening and dosing a drawer of a Stack In certain situations it is necessary to slow down the speed to open and dose a drawer Typical application example is fraction collection collecting directly into a well plate If the liquid level is high the liquid can wash over into the next row if drawer movement is too fast Remark At PAL Firmware version 4 1 X these items of the Y motor have been made available at the Extended User Level Motor Z For the Motor Z following speeds are selectable Penetr Speed This is the speed used to penetrate the syringe needle into any object with the exception of the injector Example Vial penetration Inj Penet Spd Injector Penetration Speed With PAL Firmware version 2 X and 3 0 the injector penetration speed is identical to the injector retraction speed Inj Retr Spd Injector Retract Speed With PAL Firmware version 4 1 X the injector speed was separated into a penetration and a retraction speed This allows safe penetration of a vial by any type and diameter of syringe needle without bending through the septum of the GC injector The needle retraction speed can be increased to speed up the entire injection process This new feature enables the user to avoid uncontrolled evaporation of any remaining sample solution in the needle The visibility of the Motor Z parameters at the Extended User Level was introduced wi
135. ve VICI Valco Cheminert Type 1 Remove the sample loop needle guide fitting and connection tubes from the valve body p Unscrew the Torx screw at the valve adapter which holds the valve to the valve drive 5 Check that the valve guide pin remains at its position and observe the guide marks as shown in Figure 59 4 Install the replacement valve in the reverse order PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 1 2 W Type Valve To replace and or clean the injection valve W type follow the steps shown in Figure 60 Figure 60 Replacing the Injection Valve Valco W Type 1 Remove the sample loop needle guide fitting and connection tubes from the valve body 2 Unscrew the Torx screw at the valve adapter which holds the valve to the valve drive 5 Check that the valve guide pin remains at its position A guiding mark is not stamped in in doubt check the orientation of the rotor as shown below in Figure 64 4 Install the replacement valve in the reverse order 4 1 5 Rheodyne Valve PD7991 Diameter Valve Pod 27 mm To replace and or clean the injection valve Rheodyne PD7991 type follow the steps shown in Figure 61 Figure 61 Replacing the Injection Valve Rheodyne PD7991 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA
136. verview Page 4 PAL is a registered trademark of CTC Analytics AG Switzerland note note HTS PAL HTX PAL PAL HTS x PAL HTX x PA SYSTEM User Manual Installation and Operation D How to Use this Manual This User Manual covers the HTS PAL and PAL HTS xt Systems Issues specific to the PAL HTS xtmodel have been emphasized as such Follow the description given in this manual to install and operate the PAL model HTX PAL which differs only by its X axis length of 120 cm as compared to 80 cm for the HTS PAL model and thus is not handled separately For the same reason model PAL HTX xt is also not handled separately Unless stated otherwise this manual refers to all HTS PAL models The manual is divided into the following major sections Safety Information HTS PAL Operating Instructions Specifications Product Warranty HTS PAL Description and Installation Troubleshooting Replacing Parts Maintaining PAL System Appendices The HTS PAL Operating Instructions in section E are intended for infrequent PAL users or new users who already have experience using automated systems to perform standard analytical methods The HTS PAL must be installed and set up properly before the Operating Instructions in Section E can be used Users who are installing an HTS PAL system or HTS PAL accessortes or who need to adjust an installed system should consult HTS PAL Description and Installation in Section F
137. wey Ww 05 ww G Row oO yw 123 45 67 8 9 10 1112 w o Ww o E B DI W 8 o B ww D Ww Ww E eic Ww ow ww ww P O Ian ee G w J H Figure 55 Pattern Tray Type Staggered PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt User Manual Installation and Operation syste 10 3 2 Trays The following object Tray Items can be changed by selecting the particular item First select the desired Tray such as Tray Item Tray Holder TrayTypeGroup Tray Type Offset X Offset Y Offset Z Path Offset X Path Offset Y Path Offset Z dxRow dYRow dzRow dxCol dyCol dzCol Description The Tray Holder to which the Tray is assigned is specified All Tray Type Groups which are allowed for this particular combination of Tray and Tray Holder are displayed The TrayTypeGroup for the Tray Type itself is specified for each Tray Type The Tray Type to be used is linked to the Tray This item is usually set at User Level in Utilities Trays See above The X offset is defined as the distance from the center of the teaching position from the Tray Holder to the edge of the Tray in X direction Measured in mm This item is available at User level in section Utilities Tray and can be used for fine tuning a Tray installed in a Tray Holder Important for well plates 584 installed in a Stack
138. y plate in the X axis of a column see Fig 25 for details dyCol Correction of any inclination of a Tray plate in the Y axis of a column see Fig 25 for details dzCol Correction of any inclination of a Tray plate in the Z axis of a column see Fig 25 for details Items displayed in Italic are available beginning with PAL Firmware version 4 1 X note A staggered pattern is selectable in firmware class Tray Type For details see section F Description and Installation point 10 3 1 Tray Type 2 PAL is a registered trademark of CTC Analytics AG Switzerland HTS PAL HTX PAL PAL HTS xt PAL HTX xt PA SYSTEM User Manual Installation and Operation 4 3 Injector After selecting the particular Injector to be accessed the following functions are available Function Key Description F3 Movto Inj The Injection Unit moves to the selected injector position With this function e g the injectors GC Inj1 Waste Waste2 and Flush can be accessed By selecting the parameter Needle Penetr on the same screen the Injector Needle Penetration value can be checked or changed F4 HOME The Injection Unit moves to its HOME position and the Job Queue Menu is displayed The following Injector item can be changed by selecting the particular item Item Description Needle Penetr By selecting the parameter Needle Penetr the Injector Needle Penetration value can be checked and or changed To ensure reprodu

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