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User Manual - Fisher UK Extranet

1. No Problem Possible Reason Actions Disconnected power supply Connect power No display No beeps when power Faulty fuse Replace it 250V 8A 5x20mm on Switch failure Replace it Other Contact distributor or manufacturer Turn off the power Remove the block Two beeps after power on The N Loose connection between the block from the main unit Reinstall the 2 screen displays Please insert oo and main unit block block with audible beep Turn on the power During the run the screen shows Turn off the power Remove the block Please check error or one of the from the main unit Reinstall the following options Damaged sensor or bad block 3 Temperature sensor disconnect connection Turn on the power Temperature sensor disconnect Temperature sensor2 disconnect Contact distributor or manufacturer Temperature sensor3 disconnect Turn off the power Remove the block During the run the screen shows 4 bad from the main unit Reinstall the 4 Please check error and shows SES ABER Heat sink sensor disconnect Turn the power on Contact distributor or manufacturer Turn off the power Remove the block During the run the screen shows Damaged sensor or bad connection from the main unit Reinstall Turn on 5 Please check error and indicates the power Hotlid sensor disconnect _ Contact distributor or manufacturer Ventilation Blocked Clear the blockage Abrupt change in heating spee
2. e 85 v 82 S 79 g 76 Block EH 73 70 S Reagent nmomoaonononononoaonoaonaown a EN EN E M SSE LA O EPP re 9 Time s Tube mode There is an overshoot after reaching the set temperature With the same set time as Block the actual time for the reaction to maintain the set temperature is longer Refer to the following figure showing the relationship between the block temperature and reaction temperature 100 97 94 Ee 91 _ 88 2 85 o 82 3 79 S 76 a 73 Block u 70 Reagent NONO pm OO NO NONO Lo OO Ip OO L CH IN ve e CN CDN Dm mo St st o D FF OW AD On 100 Time 5 Note After setting the parameters press Save to confirm the settings Before using the instrument re set the clock to your local time E WORLD CLASS WORLDWIDE 16 User Section Chapter 3 eRunning PCR Sample 3 1 2 4 Hot lid Hot Lid Open or closed 3 1 2 5 Key Sound and Run End Sound The sounds when pressing a key after the end of the run 3 2 HOW TO RUN A DUAL BLOCK PCR PROGRAM Turn on the power The Thermal Cycler will beep twice The MAIN WINDOW screen will display Self Testing eeeeee and the instrument will do Esco Version 2004 1 2 A 2003 11 1 10 05 00 a self test for 1 2 minutes After the self test the screen will A display the main interface Block x well De
3. e Press Cycle to repeat adding sections max 5 sections E WORLD CLASS WORLDWIDE 18 User Section Chapter 3 eRunning PCR Sample e Press Save to access file save menu e Press Back to return to File List menu Note 7 For the newly edited PCR programs save first and then run them otherwise the file cannot be run 2 When setting Time as the hot lid will close automatically when the block reaches the target temperature And the set temperature will remain until the PCR process is being stopped In this window press Digit amp Letter to access the file name Press Enter to move the cursor forward Select User and File Name with the pop up key Click the left button to delete characters while clicking right button to move the cur sor Press Caps to shift between upper and lower case Press Main to return to the main menu FILE SAVE WINDOW e Press Save to save the file When the file is saved the system Esco Version 2004 1 2 A 2003 11 1 10 05 00 will prompt File has been saved Please continue with another sane User ESCO operation Press Enter to cancel If the file has been used the system will remind you to Rename or to Save File Name PCR1 e If the current Control Mode is Block Mode press Run to en Password E Password check ter into File Run window If the current Control Mode is Tube Mode press Run and the following dialog box will appear Block x well Select Sampl
4. Table of Contents Table of Contents Warranty Terms and Conditions cccccesseeeseeeseeeseeeseneeeesseeseeeeeseesonsenaseeesenesenaseoasenssenasenssenssananens iii Drood ON eaa E AE NE KERA Ng TEGANE KAE ERA KNA v l Products OW CRC sarsii san es be dnecmenntacepetneteetiacinds caine tiauraanaintietdeqeaneeennetieeneateeatwetueriaiue ee aaniasensusiausanelas V PREE AE L A sasana E E E EE E a E PEE E E D E E E aa ee E EE ee Vv a LAS Or Eeer vii 4 European Union Directive on WEEE and Rose vii EE Ee EE EE vii eet vii 7 Important Note EEN vii De laration Of Conformity sssaaa a a Aa ERAK AKARA KENEN IK About Esco Swift MaxPro PCR Thermal Cycler aaaananenawanennnnenannnennennnnnnennennanenwnnnennnennennwenanene xi User Section Basic Product Information scccsiaccciss secede cecsececs EEN DEER deeg 1 BN VCR GV aaa aa tesa ia Gach aaa aa aa aaa aaa ncn es ah aa aaa aa aaa a a aa aaa aa aan anakan aa a aa aa a aa tata aab a a aaa 1 WA TC ag WEE 2 Lo ele ien Ga A PK EE PE EN NANA PE a a NA Ng TE EN aa E EN E a a 3 TABIO KS aaa e aaa ta agak pai aa Ga NG aia a A aa naka nga a E a ada a a a Ga EN Gaga Ng a Ga EE aa a aaa NG aa a a E Ka ak aaa 4 Ce e WE 5 os BANG ne Le EE 5 Bee Datei HN et EE 5 motala d WEE 7 2 1 Unpack Your Instrument ci sesascaniescneehseasas Pesnccccascn iets adacdnaadcaiiaksatetieadizesanhidioninaiiasidcbaaisaieedacaidabsasaniausaeane 7 E ae eege The Me ANUNG TG E E leie Rent aaa saa naas aaa AS Aa aa
5. ESCO WORLD CLASS Esco Controlled Environment Laboratory and Cleanroom Equipment Solutions NSF ANSI 49 Biological Safety Cabinets e Animal Containment Workstations e Fume Hoods e Clean Benches kd ee pn Belgigue Belgi t l 056 260 260 056 260 270 be fisher thermofisher com www be fishersci com Espana tfno 91 380 67 10 91 380 85 02 es fisher thermofisher com www es fishersci com 8 Ge Se France t l 03 88 67 53 20 03 88 67 11 68 fr commande thermofisher com www fr fishersci com Fisher Scientific Italia tel 02953 28 258 02 953 27 374 it fisher thermofisher com www it fishersci com Netherlands tel 020487 7000 020 487 70 70 nl info thermofisher com www nl fishersci com Portugal tel 21425 3350 21 425 33 51 pt fisher thermofisher com www ptfishersci com Suisse Switzerland t l 05661841 11 056 618 41 41 info ch thermofisher com www ch fishersci com Part of Thermo Fisher Scientific Switt MaxPro Thermal Cyclers User Manual Thank you for purchasing the Esco Swift MaxPro Thermal Cycler Please read this manual thoroughly to familiarize yourself with the many unique features and exciting innovations we have built into your new equipment Esco provides many other resources at our website www escoglobal com to complement this manual and help you enjoy many years of productive and safe use of your Esco products User Manual For Technical Service contact North America Esco Technologies
6. USB Link System Run __ The top left of the main window shows the Hot lid tempera ture The central area shows Instrument information software version number block model default file default user control mode and sample volume e Press File to enter file list window e Press System to enter system parameter setting window e Default Control Mode is Block Mode e If current file doesn t have password press Run to access the File Run window e If current file has password press Run to access the File Run window In this window press Character to input password Password Window e Press Back to go back to the main interface Hotlid off 2007 11 1 10 05 00 e Press Accept to confirm the password E S CO e d is mixed block dea EE choose ESCO MICRO Pte Ltd the test tube type pop up tube type selection window Version 2007 1 0 e If it is not the mixed block SWT MXP BLC 2 and the current aes Next poco S HM i efault User Control Mode is Block mode press Accept to access the file Default File PCR1 run window If the current control mode is Tube mode press Control Mode Block Accept to pop up sample volume Settings window Input password Back Accept II II E WORLD CLASS WORLDWIDE 1 2 User Section Chapter 3 eRunning PCR Sample In this window press to choose the actual tube type TUBE TYPE SELECTION e Press Back to go back to the main window Hotlid off
7. e File editing and saving e File modifying and deleting e Adjustment of the heating cooling rate e Temperature amp time increments decrements during runs e Data display at each phase of the run E WORLD CLASS WORLDWIDE 64 Chapter e Technical Data e Program pause function e Program stop e Auto restart in case of power failure and alarm e Software upgrades via RJ45 interface e Audible alarm e Total program run time and remaining program run time e Date and time year month day hour minute second display and calibration e Failure protection and alarming Swift MaxPro Thermal Cyclers e User and Service Manual Appendix 1 Wiring Diagram 65 Ab 1900 DN 3 GER geet NDA AR oA ee We ae daimia ofr dh A3 it hrs ES i sah VE E ung BETAN er H Ki com EN ont NU Ta ot DOT Neige Goy Sur MRO Odo Fr rsd gf Nei r i A Ep HISNISAOGH Eet Met rei La NE Aer DWI terp Net Fei D I REI INY i idl Par BWI L bW Les Ciir z Sa Fo NIN E sate KL bbl AL at TA EE akan H Dr panne H L I a E if Wn AE pei wi Leg hi ZE GDL IOC GEAR Ping Lou ee e d el e T ee E emm MA CIEZL DH CLASS WORLDWIDE 66 Appendix 2 e Parameter and Meaning APPENDIX 2 PARAMETER AND MEANING Item Parameter Meaning Range Note 1 Temp C Temp
8. read state read user list read file s count read system settings Swift MaxPro It will enter the main interface automatically Choose the unit named ETI you will find that the disconnect button is bright which means it is connected now and you can discount it D Baangja Re a 8888 sas 2022 aR De TT B Computer fa Note Some firewall software may block the port if the PC cannot connect the thermal cycler successfully please turn off the firewall of your computer Go to Start click Control Panel there is a Windows Firewall double click and then choose off for the firewall Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 41 4 3 MAIN INTERFACE OF MAXPRO PC SOFTWARE User Fi Local com r x NAN CN Le pute uS Ei cA xX LA LS bd E 6 a Va a ac ae anana Fitter fall si u ss Equipment state display eck S eq104 96G S Main display area 2 984 Q Setting Equipment list 4 3 1 Toolbar Change user name Import file Copy file Change file password Export User Paste user Create New file Delete file Connect SIFIL Import User Copy user Export File Paste file Save file PPPE EIIE Grad temp Server setting Create new user Delete user Edit file Change file name Disconnect 4 3 2 Operations Run a file 1
9. 100 75 Move the cursor by pressing Up Down and Right Left and the 50 position that the cursor reaches will be displayed in white on a 25 black background 0 i Press Digit amp Letter to modify parameter settings Press Func Temp 1c95 0 2c 55 0 3c 72 0 4c 04 0 tion to change but before the zero cannot be changed Time 01 00 02 00 01 45 Should you need to return to the main menu press Save first Seg Temp C Time Ramp C s Temp Time 1c 95 0 01 00 0 0 0 00 a 2c 55 0 02 00 0 0 40 00 e Press Seg to access the segment edit menu In each segment 3 72 0 01 45 0 0 0 00 l 4 4 you can set temperature Temp duration Time ramp rate S Pea Feces 0 0 0 00 Ramp amp temperature increment of each cycler Temp and ee time increment of each cycler Time Press Delete to delete the present segment Press Cycle to enter cycle edit window where you can set cycle numbers and initial cycle segments The figure shows a cycle from the first segment to the third one There are a total of 10 cycles Press Cycle repeatedly to add sections Max 5 sections Press Delete to delete the present segment Move cursor by pressing Up Down to access the former segment or the latter segment Press Save to access the file save menu Press Back to return to File List menu For a newly edited PCR program save first and then run it or the file can not be run Wh
10. COM Mapping Monitor Port Monitor AN IP Address Report Searched IP Configuration 0 Network Enabler Module s address Now 2006 1 3 14 15 25 Double click the searched IP on the column and choose the Network option Configuration NE 41105 MAL Address 00 90 E8 10 E2 B7 Serial Number 3838 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Click the Modify check box to modify configuration Auto Waming IPAddressRepott Password DigitallO Serial CMD Basic Network Serial OperatingMode AccessiblelPs v Modify IP Address 192 168 0 245 Input IP Address 255 255 255 0 Input Netmask IP Configuration Static DNS Server 1 DNS Server 2 Modify X Cancel SOK Note P address can be set as 192 168 0 XXX which means we can change the last 3 digitals from 1 to 255 If only connect to one thermal cycler you can leave it as 192 168 0 245 as default and if for more units please connect PC with every unit one by one and set for different IP addresses for each unit Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 31 Choose Operating Mode option then click Settings button Configuration x Information Auto Waning IP Address Report Password Digital IO Serial CMD Model g i NE 41108 Basic Network Serial Operating Mode Accessible IPs MAC Address vi Modify 00 90 E8 10 E2 87 D
11. ul 10 200 State of Hot Lid Off Off 14 Holid State of Hotlid Open C 30 110 15 Key Sound Key Sound Yes or No Yes No Humming 16 Run End Sound Run End Sound Yes or No Yes No Humming continuously 100 100 97 97 94 94 91 91 ae ES _ 88 85 2 85 o 82 o 82 2 79 2 79 5 76 5 76 73 73 Block 70 F 70 Reagent SC ANAMFTRRHRSESCRAKSHAAS PST RANA AMETRRESGORABHARAS Thermal Cyclers e User and Service Manual cco WORLD CLASS WORLDWIDE Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 1 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com e usa escoglobal com Esco Micro Pte Ltd e 21 Changi South Street 1 e Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 e mail escoglobal com e www escoglobal com Esco Global Offices Singapore Philadelphia USA Leiden The Netherlands Salisbury UK Kuala Lumpur Malaysia Beijing Shanghai amp Guangzhou China Mumbai India Manama Bahrain 2008 Esco Micro Pte Ltd Specifications Subject to Change ES1460_V1_09 09
12. 2007 11 1 10 05 00 e Press next If the current control mode is Block Mode click next to access file run window if the current mode is Tube E S CO Mode press next to pop up the following interface ESCO MICRO Pte Ltd Version 2007 1 0 Block KAKAK Default User ESCO Default File PCR1 Control Mode Tube Select Tube Type 0 2m ILL Back Next In this window press Character to input the sample volume Hotlid off 2007 11 1 10 05 00 Unit Micro liter SSC In Tube Mode samples with different concentrations have ESCO different temperature overshoots and run times ESCO MICRO Pte Ltd e Press Run to access the file run window Version 2007 1 0 Block WAN 3 1 1 How to edit a PCR program Default User ESCO a Default File PCR1 Press File in the main window to access file list window There Control Mode Tube are 3 files listed in the form as shown in the picture on the right When the bar in the user column is flashing select user with the pop up key At this time the File Name Column e will display the selected files Shift to the File Name Column where the bar is flashing then select the user file with the pop up key e Press Edit to edit the file as indicated by the cursor FILE LIST WINDOW e Press New File to edit a new file Hotlid off 2007 11 1 10 05 00 e Press Delete and the system will show Confirm delete User File Name Save file Select Delete or Back E e Press Back to return
13. 31 Complete 2007 11 29 10 15 37 Complete 2007 11 29 10 14 08 Complete 2007 11 29 10 13 27 Complete User Section Chapter 4 e Computer Connection 43 Check the equipment storage state Double click the System to view equipment storage state T MaxPro User File Iools Help 9 889 9 08 OB e d EEE eau ij Computer eq104 gt system state Ea Finer fan D 8 ul B ss Total memory size 51200 eql06 D okfile S fi Used memory size 3072 eq104 96G aju Free memory size 48128 S amp eq106 User Free memory scan store about 94 files e I4 System amp eqi04 User e i huang 984 D 51 ev ai E test s SA Run File Set system parameters Double click the Setting under System options and set system parameters Maer File Tools Help 223233208 2200 080R LY Sah ea f a eq104 gt system setting EF Firer lan T D ss Default eq106 okfile Default hotlid temperature OFF amp 51 Cc s s fi Beep alarm when run finish oF 53 yas ee oF eq104 96G S i wf Temperature control mode Tube gt 8 et Default user py User Default file a huang i 984 Module A 51 Default hotlid temperature ps e 52 Cc ji i Beep alarm when run finish ore D J4 System Key beep oer e E Histo Temperature control mode Biok Run File Default user D Default file fy Module B Default hotlid temperature pr C Beep alarm when run finish OFF Key beep ON Temperature contro
14. Connection Double click the searched IP and select Advanced Settings page set up as the following fig COHN Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable _ Fast Flush Only Flush Local Buffer Apply All Selected Ports COM Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Baud Rate Parity Data Bits Stop Bits Flow Control Click OK button Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 35 XN Network Enabler Administrator CON Mapping Fie Function COM Mapping View Help Kat o fh Function COM Mapping 1 COM Ba Network Enabler Adma No Model IPAddess Pot COM Port Mode Parameter Configuration NE 41105 HiPertcemance FIFO Ena 9600 None 8 COM Mapping Monitor D Pon Morito A IP Address Report 2008 1 3 14 26 33 Found Network Enabler Module s 1 20091 3 1435 18 Configuration Changed NE 41105 00 30 E810 E287 2008 1 3 14 35 33 Configuration Changed NE 41105 00430 E amp 10E287 2008 1 3 1435 49 Configuration Changed NE 41105 00 90 8 10 E2 B7 2008 1 3 14 37 22 Configuration Changed NE 41105 IO S0 E8 10 E2 B7 Click Apply Change button D complete the mapping settings to confirm the setting and then click the Remove Target eq
15. Control point 4 0 99 0 E SE Sg h The time of Temp control is infinity meanwhile gt ITE Time of Temp SEENEN J the hotlid closed Control mm ss key 00 00 59 59 Ramping Rate 0 1 4 0 C 3 Ramp C S CS d C Second HH Maximum Ramping Rate 4 Temp Temp Gain of Every Cycler 0 0 9 9 5 Time Time ee of Every Cycler 0 00 9 59 mm ss Cycle1 Section Numbers 1 5 Cycler Numbers from SR beginning to the end E 6 The start segment of the X and existed Cycle1 x00 From 00 to 00 From 00 section segment no to 00 The end segment of the X415 section 7 Grad Gradient Temp range C 0 30 8 Date Date YY MM DD SECH 01 12 01 Year Month Date 9 Time Time HH MM SS 00 00 00 23 59 59 Hour Minute Second 10 Default File 11 Default User The default user file The default user name Max length is 11 characters Max length is 11 Time s Swift MaxPro Time s characters In this mode the time for reagent s temperature Block to reach the set temperature is less than setting time see Fig 1 12 Control Mode Control Mode In this mode the reagent s temperature can Tube reach the set temperature very rapidly see Fig 2 13 Sample Volume Sample Volume
16. EAS AGA aana ga aaa 22 3 3 3 Properties of The Gradient Block 22 3 4 How To Import And Export The PCR Program 23 341 Data Tansier By Usb Memory Eege Eh 23 34 2 Import File From EE Ee 23 34 3 EXON File TO UsoFlash Memory SUCK E 24 A Computer e ET d siisii aaa a aaa a ak aaa dyan a ga aa aaa 25 A Sotware Salak DIN EN 25 4 2 NetWork Ela E E E 27 4 2 1 Network Enabler Administrator Installation cccccccccccccccceeeeeeteeeeecceccceeeeeeeeeeeeeeeeeeeeeceeeeeeeeeeauaaauaauauaaaeaaaaaaas 27 4 22 Configure Your EquipNet sessed ia ga aaa WA a Aa b ni a a a E E aaa aa aa a a gg ana an wangi an a eaaa d aa ad a 29 EN Ee in ee TO gene EE 29 4 222 RUN Network EE ee ue alt Ce awan aaa aaa SENA NANA a men a ahh na nah aaa aia aa aa SA a KA a aa a ajana a aa a A a aaa deh 29 ele 30 42a Deele sanan eaaa aaa aa AN An aa a da aa a aaa aba dada ORE A aa a eA a aa E ee eee ore KE AE a a a aaa 35 4 2 4 Add thermal cycler IP address in MaxManager PC software 38 4 3 Main Interface of MaxPro PC Software 41 GW ee 41 OZ OS e KA sca ot a rises et arse sles pa deve AK AN MAN ete cg cd mind wnt ened ee 41 A U NON EE 44 AA OT EE 45 o CC Tee BEE 48 5 Equipment Software Upgrade cccscssscssscessessseensesseenseenseoaseossesseossenssensseessenseonssoeseonseons 51 Rea de Your CIO E 51 5 2 How To Upgrade Ee e 51 6 Routine Maintenance see KENE EEN K EE EE ENEE EEN EE ENER EE KEE REENEN EE EE ENER KE a 59 7 Failure Analysis
17. Gradient PCR Touch Down PCR etc User Section Chapter 3 e Running PCR Sample 19 SYSTEM PARAMETER SETTING MENU Version 2004 1 2 A 2003 11 1 10 05 00 Default User ESCO Default File PCR1 Control Mode Block Date 03 10 22 Key Sound Yes Time 14 53 19 Run End Sound Yes Hotlid 105C Block x well Default User ESCO Default File PCR2 Control Mode Block Defautt __ Back Save Note 7 After setting the parameters press Save otherwise the parameters will not be saved 2 Reset the internal clock to your local time 3 3 HOW TO RUN A GRADIENT BLOCK PCR PROGRAM Turn on the power The Thermal Cycler will beep twice The screen will display Self Testing eeeeee The self test is about 1 2 minutes following which the screen will display the main menu e Press File to enter file list menu e Press System to enter system parameter set menu e Ifthecurrent file doesn t have password press Run to access File Run window If the current file has password press Run to pop up following dialog box Input password MAIN MENU Hot lid off 2003 11 1 10 05 00 ESCO ESCO MICRO Pte Ltd Version 2004 1 2 Block x well Default User ESCO Default File PCR2 Control Mode Block As same as single block press Back to go to the main window Press Accept to confirm the password e If the Control Mode is Block Mode press Run to enter the File Run menu If the Co
18. Inc 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040 USA Toll Free USA and Canada 877 479 ESCO Tel 215 441 9661 e Fax 215 441 9660 us escoglobal com e usa escoglobal com Rest of World Esco Micro Pte Ltd Swiftt MaxPro 21 Changi South Street 1 e Singapore 486 777 Tel 65 6542 0833 e Fax 65 6542 6920 www escoglobal com e mail escoglobal com Thermal Cyclers US 50 00 Europe 40 00 Additional manuals can be purchased through Esco SWT MXP User amp Service Manual Version 1 0 your Esco Distributor Released October 2008 COPYRIGHT INFORMATION Copyright 2008 Esco Micro Pte Ltd All rights reserved The information contained in this manual and the accompanying product is copyrighted and all rights are reserved by Esco Esco reserves the right to make periodic minor design changes without obligation to notify any person or entity of such change Swift is registered trademarks of Esco Material in this manual is provided for informational purposes only The contents and the product described in this manual including any appendix addendum attachment or inclusion are subject to change without notice Esco makes no representations or warranties as to the accuracy of the information contained in this manual In no event shall Esco be held liable for any damages direct or consequential arising out of or related to the use of this manual Swift MaxPro Thermal Cyclers e User and Service Manual
19. MaxPro thermal cyclers be connected and controlled by normal computer Software upgrades via RJ45 as well E WORLD CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 1 Basic Product Information CHAPTER 1 BASIC PRODUCT INFORMATION 1 1 QUICK VIEW E emm MA CIEZL DH CLASS WORLDWIDE User Section Chapter 1 Basic Product Information Description With hot lid heat sink and heating amp Five interchangeable blocks are available Block cooling system for different requirements 5 rep screen Display protocol conditions and other in formation during setting and running 3 Keyboard For setting and controlling 4 Ventilation vent S REEGELEN Don t block off ventilation vent Data transfer between USB and equip SE ment through USB interface Turn the knob counter clockwise before 6 Hot lid knob Adjust the height of the hot lid desire eae Fel Nev et the Aok A e tightened by turning it clockwise and can be loosened by turning counter clockwise If hear click sound at the beginning of turning the knob clockwise and counter 7 Unlocking Device Loose the hot lid knob clockwise press the unlocking device and turn the knob counter clockwis
20. Open the file of the equipment or the computer 2 Click Run button 3 Select Tube type and tube Volume 4 Click OK button to run the file Note f the file is saved in the computer choose the equipment before running ESO CD OOL CLASS WORLDWIDE 442 User Section Chapter Ae Computer Connection Check the running file 1 If the equipment is running Run File A B will be showed under equipment name 2 Double click Run File A B display the running file content and equipment running states Note Click Pause Stop button to pause stop equipment running ee File Toole Yelp KEE KEREN EL WEIER FGA eo a Computer agli gt Run File aff ul Bes D okfla H Pier a Cuvee volume 60ul Cuvetie ypec0 5ml State Running Tote tne h Zim Remaining time MMh lm Hogg emp 20 50 Module temp 20 7C Curent Segment Ramaming Time 00 20 54 55 00 r H EEA U AD secs 52 00 Ae 1 A000 pen 50 peca Check history information Double click the History under the System option to show the history information Yser File Tools Help 888898028 221420546332 TIGR on ZS Fiter an D Computer eq104 gt file run log aul D ss okfile fl aju 8 eg106 User I System 8 eqil4 Swift MaxPro 2007 11 30 15 57 22 2007 11 29 10 15 53 2007 11 29 10 14 17 2007 11 29 10 13 39 2007 11 29 10 12 52 Thermal Cyclers e User and Service Manual 2007 11 29 10 16
21. Section Chapter 3 eRunning PCR Sample 3 3 2 How to set system parameters SETTING SYSTEM PARAMETERS In the main menu press System to access the system parameter Hotlid off 2007 11 1 10 05 00 setting menu Date 03 10 22 Press Enter to move the cursor to next item The option is fol Time 14 53 19 lowed by the symbol 44 Press Up Down to scroll to select Default User ESCO ft Other information can be entered by pressing Digit amp Letter Default File PCR1 If Press Right Left to move the cursor Control Mode Block e Press Back to return to the main menu One E Key Sound Yes e Press Save to save parameters Run End Sound Yes e Press Default to restore the default files Standard PCR Default Gradient PCR Touch Down PCR etc Note After the parameters have been set press SAVE or the parameters will not be saved 3 3 3 Properties of the Gradient Block The gradient block uses a 3 group peltier unit The gradient temperature appears as a curve distribution as shown in the following figure Set temp is 40 C and the gradient set temp is 20 C The gradient temperature distribution among the individual wells is not even The temperature difference in the middle of the block is greater than that of the extremities Refer to the temperature displayed on the instrument for individual well s temperature when operat ing the block Gradient Graph 5 6 7 8 Column left to right Swift MaxPro Thermal Cyclers e U
22. and Troubleshooting 0nesneeneeneenen nee nen nen nee nen nen EENREER KEREN KEEN REENEN 61 5 T NAGA DATA E 63 EE ee SE BZ Fl edu AI NC EE 63 8 3 EQUIPMENT Software FUNCTION scccd ccnes snciciiesndecndasdendectectestenecpacnneseets lt ceceedeededensdenseedexceaentenesscedtennmsestesdecesoe 63 Appendix Ze Wiring Diagrami asasi aaa au aaa aaa ia a a aaa aira a ina aa aaa ajin a BN a raja a aa aia A daja a a aia a a mn a a a ajaka wia a aa an pa aa 65 2 Parameter amp Meaning eege 66 SwifteMaxPro Thermal Cyclers e User and Service Manual Warranty Terms and Conditions II WARRANTY TERMS AND CONDITIONS Esco Life sciences products come with the following limited warranty commencing from the date of shipment from Singapore e 24 Months on the Blocks and Peltier Units e 36 Months on all electronic components e 36 Months on the housing and fans Esco s limited warranty covers defects in materials and workmanship Esco s liability under this limited warranty shall be at our option to repair or replace any defective parts of the equipment provided if proven to the satisfaction of Esco that these parts were defective at the time of being sold and that all defective parts shall be returned properly identified with a Return Authorization This limited warranty covers parts limited transportation and does not cover insurance charges For all peltier relater warranty claims e The complete block assembly must b
23. cycler press F1 and hold for a while LCD displays Prepare to upgrade eee system enter software upgrade state 2 Run Network Enable Administrator Equipment will use virtual equipment mapping for communications during upgrades Configuration Broadcast button N Het work Enabler Adsinistrator Configuration Seg Fie Function Configuration View Help 223 eege Function Configuration 0 Network Enabler Module s BY Network Enabler Admi No Model MAC Addtess IPAddress Status Eh Configuration Mapping 6 COM Mapping Momtor Port Morgo XE IP Address Repost Function Mow 2008 1 3 14 15 25 E emm MA CIEZL DH CLASS WORLDWIDE 52 Chapter 5 Equipment Software Upgrade 3 Configuration Click the Broadcast button the software will search equipment automatically w Hotwork Enabler Administrator Configuration File Funchon Configuration Wew Help Configuation COM Mappang Maritor Port Maria AM UP Address Repat Searched IP Configuration 0 Network Enabler Module s 1105 13 16S 0 285 1 iA address NE 41105 MAC Address 00 90 E8 10 E2 B7 Serial Number 3838 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Swift MaxPro Ree 3008 1 3 14 15 25 AutoWaming IPAddessRepot Password Digitall0 SerialCMD Basic Network Serial OperalingMode AccessiblelPs v Modify 192 168 0 2
24. decompilation o Hanz schen of the Systemin E Whole Of ri paiia re allowed Waranty The computer program shall be under the protection of Capyight Law and Irdemabonal Lenegpart Ary whole of paitial copy decompalahon or spread of thes Geo am prar bo F Do pou Sccepnt al the berme of the preceding License Agreement If pou Pint select No the setup will close To natal Max Pro program you mus accept this ageert Click Yes Button to accept all the terms of the agreement Max Pro program Setup Customer Information Please enter your information Please enter your name the name of the company for which you work and the product serial number User Name PCR Company Name ESCO MICRO PTE LTD Serial Number 123 lt Back Next gt Cancel E emm MA CIEZL DH CLASS WORLDWIDE 26 User Section Chapter 4 Computer Connection Complete your information and click Next Button Max Pro program Setup Choose Destination Location Select folder where setup wi install files Setup wi install M ax Proprogram in the following folder To install to this folder cick Next To install to a different folder cick Browse and select another folder Destination Folder C Progeam Files ESCO M axPro program Browse lt Back Net gt Cancel Choose the installation destination folder and then click Next button the default folder is C Program Files ESCO MaxPro pro
25. e NEAT105 Basic Network Serial Operating Mode Accessible IPs MAC Address v Modify 00 90 E8 10 E2 B7 De d COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Click the Modify check box to modify configuration SOK X Cancel Choose TCP Server Mode for Operating Mode Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Choose TCP Real COM Mode Server Mode TCP Server Mode TOP Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout H 0 99 min Inactivity Timeout Max Connection 1 0 0 65535 ms Data Packing Optional Delimiter 1 00 0 ff Hex Delimiter 2 00 0 ff Hex Force Tx Timeout 0 0 65535 ms SOK X Cancel Click OK button close Network Enable Administrator 12 Restart the thermal cycler equipment enter the system parameter window to reset system parameter E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual Chapter 6 eRoutine Maintenance 59 CHAPTER 6 ROUTINE MAINTENANCE Maintaining your Thermal Cycler Cleaning the Sample Wells e Turn off the instrument and let the instrument cool for 10 15 minutes e Thoroughly clean wells with a cotton swab soaked in 100 Isopropanol e If wells are contaminated clean thoroughly with a cotton swab soaked in
26. move backward a bit e Pull the locking handle to move block gently towards the rear of the main body e Remove the block from the main body Note Turn off the power supply before installing block When installing the block pay close attention to pushing the block slowly and gently or the main unit s connecting plug may be damaged When uninstalling the block please pull the block from the block bath gently Do not tilt the block or the connection plug may break Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 2 Installation 9 2 3 2 Inspection before Power on Before switching the power on please ensure that 1 The supply voltage falls within the specified limits 2 The plug has been inserted into the power socket 3 The power line is grounded 2 3 3 Power on Turn on the power i e turn the power switch to position The Cycler will beep twice and the LCD will display Self testing eeeeee The instrument will self test for about 1 2 minutes After the self test the main menu will be displayed and the instrument is ready for use E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 3 e Running PCR Sample 11 CHAPTER 3 RUNNING PCR SAMPLE There are five block types for this cycler In this chapter we will describe in detail the operation of each block type including how to edi
27. 000 000000 0600 60660006 0800 O00000 009000 6660006 000 30 0 47 3 31 7 49 9 33 7 52 2 E 53 4 39 7 54 0 Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 49 User File Teols Help RX CEET aeRVLa TFT ado Computer Grad Temp 3 8 12 GA B 34 Temp 39 Gredientfaa C 8x12 C 16X24 Cac Colder Hotter 0000008088088 OC 60660666 000000000000 EE EE EE Server Setting Change the equipment IP and the name 1 Choose Server Setting under Tools menu pop up the setting dialog box 2 Click the Add button to add a row 3 Click the Delete button to delete the current row 4 Putthe IP address and alias click Save button to save the setting Note 7 7he setting will be effective until the software restart 2 IP setting should be corresponding to the equipment Server Setting 192 168 0 222 192 168 0 243 amp Delete 192 168 0 240 192 168 0 241 E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual Chapter 5 e Equipment Software Upgrade 51 CHAPTER 5 EQUIPMENT SOFTWARE UPGRADE 5 1 CONFIGURE YOUR EQUIPMENT Connect the equipment to computer through RJ45 interface Cross Class B Connection e Computer RJ45 interface Instrument 5 2 HOW TO UPGRADE THE SOFTWARE 1 Power on thermal
28. 45 255 255 255 0 Static DNS Server 1 DNS Server 2 Modify Click the Modify check box to modify configuration Thermal Cyclers e User and Service Manual Input IP Address Input Netmask Chapter 5 e Equipment Software Upgrade 53 Choose Operating Mode option then click Settings button Configuration E Information Auto Warming IP Address Report Password DigitallO Serial CMD Model z e Wee Basic Network Serial Operating Mode Accessible IPs MAC Address v Modify 00 90 E8 10 E2 B7 i Del COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Click the Modify check box to modify configuration J OK xX Cancel Choose Real Com Mode for operating Mode Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TCP Server Mode Choose Real Real COM Mode Com Mode TCP Server Mode TOP Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout 7 0 39 min Inactivity Timeout Max Connection 1 0 0 65535 ms Data Packing Optional Delimiter 1 00 0 ff Hex Delimiter 2 00 0 ff Hex Force Tx Timeout 0 0 65535 ms E emm MA CIEZL DH CLASS WORLDWIDE 54 Chapter 5 Equipment Software Upgrade Click COM MAPPING from the function list Add Target equipment Remove Target equipment XN Network Enabler Administrator
29. COM Mapping File Function COM Mapping View Help Apply change 2008 1 3 14 26 33 2008 1 3 14 35 18 2008 1 3 14 35 39 2008 1 3 14 35 49 2008 1 3 14 37 22 COM Mapping 0 COM No Model IPAddress Pot COM Pot Mode Parameter Found Network Enabler Module s 1 Configuration Changed NE 4110S 00 30 E amp 10E287 Configuration Changed NE 4110S 00 S0 E8 10E287 Configuration Changed NE 4110S 00 30 E8 amp 10 E287 Configuration Changed NE 41105 00 90 E8 10E287 Fie Function COM Mapping View Help Dep eg Function Qe IP Address Repost 2008 1 3 14 26 33 20081 3 14 35 18 2008 1 3 14 35 39 2008 1 3 14 35 49 2008 1 3 14 37 22 Swift MaxPro Thermal Cyclers e User and Service Manual COM4 Found Network Enabler Module s 1 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Configuration Changed NE 41105 00 90 8 10 E2 B7 Configuration Changed NE 41105 00 90 E8 10 E2 B7 Hi Pestormance FIFO Ena 9600 None 8 1 Nor Chapter 5 e Equipment Software Upgrade 55 Double click the searched IP and select Advanced Settings page set up as the following fig CON Port Settings Port Number 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Tx Mode Classical FIFO Enable _ Fast Flush Only Flush Local Buffer Apply All Selected Ports CON Port Settings Port N
30. Hotlid off 11 705 Delete to delete the current segment Move the cursor A TT TTT by pressing Up Down to access the previous or latter seg 100 i ment 5 gt 50 i e Press Save to access the FILE SAVE menu 25 e Press Enter to check the gradient details There will be 0 no response if the Grad is 00 Please refer to the follow Temp 2c 55 0 3c 72 0 4c 04 0 ing picture Time 02 00 01 45 e Press Back to access the file run window Seg Temp C Time Ramp C s Temp 1c 95 0 01 00 0 0 1 2 9 10 950 951 966 968 Note For newly edited PCR programs save first then run or the files cannot be run When setting Time as the hot lid will be closed automatically when the block reaches the target temperature And the set temperature will remain untill the PCR process is being stopped In this menu press Right Left to access the file name Press Enter to FILE SAVE WINDOW move the cursor forward one position Select User and File Name Hotlid off 2007 11 1 10 05 00 with the pop up key Click left button to delete characters Click the right button to move the cursor Press Caps to shift between User ESCO upper and lower Press Main to return to the main menu l File Name PCR1 e Press Save to save the file When the file is saved the system will prompt File has been saved Please continue another operation Press Enter to cancel the pr
31. IMPLIED WAR RANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY NO WARRANTIES WHETHER EXPRESS OR IMPLIED WILL APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED ESCO DOES NOT ACCEPT LIABILITY BEYOND THE REMEDIES PRO VIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL INDIRECT CONSEQUENTIAL OR INCIDENTAL DAMAGES INCLUDING WITHOUT LIMITATION ANY LIABILITY FOR THIRD PARTY CLAIMS AGAINST YOU FOR DAMAGES FOR PRODUCTS NOT BEING AVAILABLE FOR USE OR FOR LOST WORK ESCO S LIABILITY WILL BE NO MORE THAN THE AMOUNT PAID FOR THE PRODUCT THAT IS THE SUBJECT OF A CLAIM THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE E emm JRALT CLASS WORLDWIDE Iiv Warranty Terms and Conditions These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and shall be subject to the exclusive jurisdiction of the courts of Singapore Technical Support Warranty Service Contacts Toll Free USA and Canada 877 479 ESCO Singapore 65 6542 0833 Global Email Helpdesk support escoglobal com Visit http www escoglobal com to talk to a Live Support Representative Distributors are encouraged to visit the Distributor Intranet for self help materials Policy updated on 30th Jan 2007 This limited warranty policy does not apply to products purchased before 30th Jan 2007 Swift MaxPro Thermal Cyclers e User and Service
32. Manual Introduction V INTRODUCTION 1 PRODUCTS COVERED This manual is applicable and specific to the following Esco products Swift MaxPro Thermal Cycler SWT MXP with following blocks Rated voltage V 96 mg 30x Se 48 x 384 pea A8 x 0 Se dual 4 slide in situ plate Wa SWT MXP BLC 1 SWT MXP BLC 2 SWT MXP BLC 3 SWT MXP BLC 4 SWT MXP BLC 5 2 SAFETY WARNING During operation maintenance or repair of the Thermal Cycler the following safety measures should be followed Esco shall not be in any way responsible for the consequences resulting from the improper use of the instrument of from failure to heed these warnings a Grounding Considerations To prevent electric shock the instrument must be grounded b Keep Away from Electric Circuits The operator must not open the Thermal Cycler Changing components or adjusting certain parameters insidethe instrument must only be done by a trained and qualified service engineer Do not change elements while the power is still on c Power Requirements Before turning the power on always check that the mains voltage falls within the required A C 110 220V 410 difference is allowed and whether the current rating of the power socket meets the required specification Le 600W MAX d A C Power Line Considerations Use the power cable that is supplied with the instrument Should this be damaged the power cable may not be repaired and must be replaced by a new one e Co
33. Mode is Tube Mode press Run The following dialogue box will appear Select Sample Volume 050ul The user can input Sample Volume Unit Micro liter through the main menu Press Back to return to File List menu Press Run to access the file run menu Note The sign at the top of User and File shows that the current run file is the default file This can be changed through the parameter set menu In this menu move the cursor by pressing Up Down and Right FILE EDIT WINDOW Left The position of the cursor is displayed in white on a black Hotlid off 2007 11 1 10 05 00 SERGE Temp C Time Ramp C s Temp Time Press Digit amp Letter to modify parameter settings Press Function SE SE er ge Geo to change but before a zero cannot be modified Should you 72 0 01 45 40 0 0 00 need to return to the main menu first press Save then press Main 04 0 0 0 0 00 e Press Seg to access the segment edit menu In each seg Cycle1 x10 From 01 to 03 ment the temperature Temp duration Time Ramp rate B Block x well Ramp temperature increments and time increment of each Default User ESCO cycler Time Default File PCR2 Control Mode Block e To delete the current segment Press Delete e Press Cycle to access the cycler edit menu from where you can set cycle numbers and initial cycle segment The figure shows cycles from the first to the third segment There are a total of 10 cycles
34. Run to access the file run screen Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 3 e Running PCR Sample 17 3 2 1 How to edit a PCR program FILE LIST WINDOW In the main menu press File to enter file list menu ESCO Version 2004 1 2 A 2003 11 1 10 05 00 There are 3 files listed in the table as shown in the picture When User File Name Save the bar in the User Column is flashing select the users with the Time pop up key At this time the File Name Column will display the ESCO1 PCR1 2003 11 1 files of the selected users Go to the File Name Column where the ESCO penal 2003 11 1 bar is flashing Select user file with the pop up key a Escos pers aen 1 ESCO3 PCR3 2003 11 1 e Press Edit to edit the file highlighted by the cursor Block x well Default User R Default File PCR2 Control Mode Block e Press New File to edit a new file e Press Delete The system will prompt Confirm delete file Select Delete or Back e Press Back to return to the main menu e If the current file doesn t have password press Run to access File Run window If the current file has password press Run to pop up following dialog box Input password As same as single block press Back to go to the main window Press Accept to confirm the password e Ifthe current Control Mode is Block Mode press Run to enter into the File Run menu If the current Control
35. Tasks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing Network Enabler Administrator then click Next Additional icons pistacncraraceseatesesnssaresecacesescssareseseasass ARA AKARANA NA AA BAH E WORLD CLASS WORLDWIDE 28 User Section Chapter 4 Computer Connection Click Next button GA Setup Network Enabler Administrator Ready to Install Setup i now ready to begr inaialing Network Enabler Administrator on pour TOTAL Click Install to continue with the installation of cick Back if you want to review of change any settings Destination location C Program Fies Network Emaibber Achmet ator Schaal taska Additional icon Create a desktop icon x A Click Install button ul Setup Hetwork Enabler Administrator installing Pieace wat while Setup matalik Network Enable Adminetrato on your computer Extech fies C Program Files Netvaok Enabler Adminstrator WWE ADIN ee Completing the Network Enabler Administrator Setup Wizard Setup has finished installing Network Enabler Administrator on oan oomguter The application may be launched by selecting the installed icons Chek Fmgch to eat Setup e Launch Network Enabler Adhrunntrator Firush Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 29 4 2 2 Configur
36. a 70 Ethanol solution e Rinse wells with de ionized or distilled water e Wells should be completely dry before using instrument Cleaning the Hot lid e Turn off the instrument and let the instrument cool for 20 30 minutes e Gently wipe the bottom of the hot lid with a cloth soaked in 100 isopropanol e If the hot lid is contaminated wipe the bottom of the lid with a cloth soaked in a 70 Ethanol solution e Rinse with de ionized or distilled water e Wait for the lid to dry before returning the lid to its normal position If necessary the body of the Thermal Cycler can be cleaned with a soft damp cloth When cleaning the instrument should be turned off Do not use detergents or corrosive substances to clean the instrument Running the Temperature Verification and System Performance Tests These are designed to test the following specifications Heating Rate Cooling Rate Hold Time Overshoots amp Undershoots Spread Accuracy The test requires the use of the Cyclertest Driftcon system which is a temperature monitoring system that measures the above parameters in real time An instrument that fails the tests may require service by a qualified Esco engineer Please contact your distributor or Esco E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual Chapter 7 e Failure Analysis and Troubleshooting CHAPTER 7 61 FAILURE ANALYSIS AND TROUBLESHOOTING
37. aka aan ga ete naa on Pa a a SADEAN a a DNA KE BIR A E E a Ba An A A 7 e DINO PIT anaa usapi aana aapa nan ya edocs eee an IN oes babana dan senna ee Sate A napakaken A Sa DN agak a aaa an a ia de Saat anaha na UDAN a Kh daana baa a 7 2 2 Installation Location Reouirement REENEN KENNEN ENER 7 ENEE EE ee 7 22 4 NDHING e 8 Le DINDING Seen ert re ee eee aa NA een eee ance a eine at eee E Naga 7 2 2 3 Transportation and St rage nee EE 8 DP AN NAK ee 8 2 3 Installing dei il Cu inn sasa sasanga iane aga daa aa SAKE Aa kaga KAKA GA aga weak gan NAK kaa na aa aa aaa Ek Naikin 8 E E MS ANAL VON Ne BNO CK EE 8 2 32 Inopecion Berore FO 0S e D 9 E OTH ID ninang saa SANA a gana aaa ga tos kaa aa haa E nang aaa Ngina AA A age ga aia a E ecto ngapaa apaa ses so aana a aa e aaa aana aki 9 Running Ne AE VI EE 11 3 1 How To Run A Single Block PCR Program EE 11 Be lial TOW NO ae Ne lee Ber COC En E 12 3 1 2 How To Set System Parameters cccccccsseseceeecsssssseeenceseseeeeeceeseeeeeecceusgeeesesseesseeeeceenaueeeeeeseeessseeesseneaees 15 3 2 How To Run A Dual Block PCR Program 16 3 2 1 How To Edit A PCR EE se aaa aaa eaaa eaaa aa aaa anana aana hase sscheosenencsactanqiaieennarmnndonene 17 3 2 2 How To En In 19 ESO WORLD CLASS WORLDWIDE Table of Contents 3 3 How To Run A Gradient Block PCR Program 19 Dis eel HOWw WS CI le E EE 20 3 3 2 HOW TO Set System PAPAS TCLS 2 sasa rinana ka aa naa ea a aa iiA EN AAA EA EH Aa KN Na Ag a AAS
38. an lead to possible personal injury or death WARNING Important note Read and understand before processing Important safety related points will be marked with this symbol 6 LABELS Ce gt E C Le d N ECCO Micro Pte Ltd Micro Pte Ltd Name Swift MaxPro Thermal Cycler Model SWT MXP SWT MXP BLC zb Block SWT MXP BLC Name Base for Swift MaxPro SN MXPBLCO1 09 Thermal Cycler Date C 4 Base SWT MXP Ka e Power 100 240V 600W 50 60Hz Fuse AC250V 8A 5 x 20 Sort Class IP20 HOT SURFACE E ae There are two warning signs HOT SURFACE During the program runs and immediately thereafter the metal parts close to these warning signs on the block and hot lid should not to be touched Swift MaxPro Thermal Cyclers e User and Service Manual Introduction VII 7 IMPORTANT NOTE For research use only Not for diagnostic use Esco Micro Pte Ltd is not responsible for the improper use of this instrument E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual Declaration of Conformity X Declaration of Conformity In accordance with EN 45014 1998 We Esco Micro Pte Ltd based at 21 Changi South Street 1 Singapore 486777 Tel 65 65420833 Fax 65 65426920 declare on our sole responsibility that the product Equipment Swift MaxPro Thermal Cycler Mode
39. and Service Manual User Section Chapter 4 e Computer Connection 45 Import user 1 Choose local computer 2 Choose Import User under the User menu 3 Choose the import user name click the open button Note When the user name exists and user s file is opened the user can not be imported Export user 1 Choose the user under local computer 2 Choose Export User under User menu 3 Choose the target path click OK button Note When the user s file is opened the user can not be exported 4 3 4 File menu Create new file 1 Choose the user whom create new file for 2 Choose Create New File under File menu pop up the dialog box 3 Enter the file name and password click OK button Create File Do you want to create new file in 1 of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only numbers Enter password again Use empty password Jf OK X Cancel Edit file 1 Double click the equipment user double click the file and pop up the file edit window Note f there is a password for the file need to enter password before edit the file 2 Edit the file content click Save to save the file E emm MA CIEZL DH CLASS WORLDWIDE 46 User Section Chapter Ae Computer Connection A MaxPro 22 3 8 3R000R8R00M TT EA ampun Computer gt 1 gt 1 Segment setup G ste Sement End Sement Cy
40. as been widely applied to all fields of life sciences since its invention by Mullis in 1983 and the discovery of the thermostable DNA polymerase by Erlich in 1989 Great achievements have been obtained in PCR s applications in such areas as cytology virology oncology genetics forensics and immunology PCR technology is a milestone in the history of modern molecular biology 2 Application Area of PCR Technique Research Areas Gene cloning DNA sequencing mutation analysis gene recombination and mutagenesis identification and adjustment of DNA sequence of protein structure detection of gene extension construction of synthetic genes construction of cloning and expression carrier detection of polymorphism of a gene s inscribed enzyme Clinical Diagnoses Bacteria spirochaeta rickettsia diphtheria bacillus colon bacillus dysentery bacillus and clostridium Virus HTLV HIV HBV HCV HPVS EV CMV EBV HSV measles virus rotiform virus B19 virus and Lhasa virus Parasite malaria Human hereditary diseases Lesh Nyhan syndrome hemophilia BMD and DMD Immunology HLA locus typing qualitative analysis of T cell receptor or antibody diversification immune body gene mapping quantitative analysis of lymph genes Human Genome Project Identification of DNA markers using discrete repetitive Sequence construction of a genetic linkage map detection of DNA polymorphism or semen mapping Construction of a p
41. butor or manufacturer Failure to set hot lid temperature a ma GE but set the hot lid as OFF H hot lid 12 Reagent Ih NENGGE NONA BES The reaction tubes are placed un Adjust the location of reaction tubes evaporated evenly and place the tubes evenly The lid of reaction tubes are not Please close the lid of tubes tightly closed tightly before put them into the instrument ee Failure to press Save button after Please Press Save button to save the 13 The setup of parameters is invalid the setup of parameters parameter Note 7 During warranty terms please contact distributor or manufacturer if there is a need to open the equipment to check a failure listed in the above table 2 Each instrument with the same or different brand has their own control temperature features including cooling amp heating rate stability and fluctuation Meanwhile the biological experiment has its own features such as indeterminacy and easy to be affected by the surroundings therefore any PCR program that is achieved on one instrument success fully maybe cannot be achieved the same successful result Once you change instruments to do the same experiment you d better adjust the PCR running program in order to get an ideal result Swift MaxPro Thermal Cyclers e User and Service Manual Chapter 8 e Technical Data O3 CHAPTER 8 TECHNICAL DATA 8 1 SPECIFICATIONS Ambient temperature is 10 C 30 C however the device characterist
42. cle Times E Circle setup MOLDE ea ipen ben ane ana aaa kana MA hs JAG Seg A Seg 720C 01 00 Sep Segi 550C S aE 01 00 zeg 550C 50 00 01 00 Segi 40C Protocol graphic Copy file 1 Choose the file 2 Choose Copy File under File menu Paste File 1 Choose the user under the equipment list 2 Choose Paste File under File menu Note User can paste file between computer and equipments When the file exists and it is opened can not overwrite the file Delete File 1 Choose the file need to be deleted 2 Choose Delete File under the File menu Note Opened file can not be deleted Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 47 Change the file name 1 Choose the file 2 Choose the Change File Name under File menu 3 Enter file name click OK button Change File Name Do you want to change file name of Computer gt TTTT gt 1 Enter new file name The file name can contain only letters and numbers Jf OK x Cancel Note Opened file s name can not be changed Change the file password 1 Choose the file 2 Choose Change File Password under File menu pop up dialog box 3 Enter the old password the new password and click OK button Change File Password Do you want to change password of Computer gt TTTT gt 1 Enter old password Enter new password The password can contain o
43. d 6 or poor temperature control ac ANAN NG Open the instrument check that the curacy connectors are connected properly Cooling module failure Contact distributor or manufacturer The ambient temperature or humid a ity is too high which is beyond the Please regulate the ambient tempera Significant change in cooling normal working condition of the ture and the humidity 7 speed or unavailable temperature instrument below ambient one Cooling module failure iG Contact distributor or manufacturer Fan failed or stopped Faulty temperature sensor Ea 8 Heating and cooling terminated Contact distributor or manufacturer Malfunction of all cooling modules Set the hot lid as OFF in the sys Set the hotlid s state as a certain tem tem parameter setting window perature value The control temperature time is set as in the file edit interface Set the temperature control time in which lead to the hotlid closes auto digital 9 Lid does not heat matically Loose connection between connec tors Lid heater failure Lid sensor failure Contact distributor or manufacturer E WORLD CLASS WORLDWIDE 652 Chapter 7 e Failure Analysis and Troubleshooting Abnormal characters displayed in Bad contact between chip and its socket 10 Contact distributor or manufacturer the LCD Chip malfunctioning 11 Inactive keys Film panel damaged Contact distri
44. e Volume Default User ESCO Du Default File PCR2 Control Mode Block The user can input Sample Volume Unit Micro liter the same way as before Press Back to return to the File List menu Press Run to save Run access the file run menu Note Please set password as 0 9 number characters When the files overlap the system will show File run over FILE RUNNING WINDOW e Press Stop Confirm stop running Select STOP or START Version 2004 1 2 A 2003 11 1 10 05 00 e Press Pause Pause run Select START to continue If File Name PCR1 User you don t make a decision within 100 seconds the system Temp C will automatically continue runnin SV 95 0 01 00 01 e Press Skip to stop current temperature and run the next Total Time 00h 00m Remain Time 00h 00m _ Hotlid 042C B Block x well Default User ESCO Default File PCR2 Control Mode Block Swift MaxPro Thermal Cyclers e User and Service Manual 3 2 2 How to set system parameters In the main menu press System to enter system parameter set ting menu Press Enter to move the cursor to next item The option is followed by the symbol Press Up Down to scroll to the relevant the option Other information can be entered by pressing Digit amp Letter Press Right Left to move the cursor s position e Press Back to return to the main menu e Press Save to save parameters e Press Default to restore the default files Standard PCR
45. e d COM Mode Serial Number 3898 Firmware Ver Ver 4 0 BIOS Ver Ver 2 1 Status Data Mode Click the Modify check box to modify configuration Jw OK X Cancel Click the pop up button on the right side of Operating Mode and Choose TCP Server Mode Operating Mode 1 Port s Selected 1st port is Port 1 Operating Mode TOP Server Mode Choose TCP Real COM Mode Server Mode TCP Server Mode TOP Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout H 0 99 min Inactivity Timeout Max Connection 1 0 0 65535 ms Data Packing Optional Delimiter 1 00 0 ff Hex Delimiter 2 00 0 ff Hex Force Tx Timeout 0 0 65535 ms JS OK Cancel Single click OK button IP address setting is finished E emm MA CIEZL DH CLASS WORLDWIDE 32 User Section Chapter4eComputer Connection Choose Serial Option tick Modify and choose the showed information as below to make it in blue And then click Setting button Auto Waming IPAddress Repot Password Dead SerialCMD Basic Network Advanced Network Serial Operating Mode Accessible IPs 00 90 E 8 19 26 34 TO N 8 1 Ne Fovactii Senal Number 622 Firmware Ver Ver 4 1 BIOS Ver Ver 21 Status Data Mode Set up as the following Fig 1 Port s Selected 1st port is Port 1 7 Apply port alias to all selected port Port Alias Baud Rate Par
46. e for two rounds to loose the knob Up to 30 Swift MaxPro thermal cyclers can 8 RJ45 interface For PC control and software upgrades be connected and controlled by normal computer through RJ45 interface Power Switch Turn the equipment ON OFF 10 Power Socket Connect power supply 11 Fuse Socket 1 2 KEYBOARD Swift MaxPro Thermal Cyclers e User and Service Manual Install fuse in the socket to protect equip ment from over current aus The fuse specifications are as follows 250V 8A 5x20 Fuse should be replaced by one that complies with these specifications v User Section Chapter 1 e Basic Product Information 3 1 3 PROGRAM KEYS Digit amp Letter Select the letter by repeatedly pressing the button Function Press when editing cycling temperature and time Press to set infinity time for a certain temperature Shift Shift from one block to another and from Capital to Lowercase Right Left Moves the cursor one position to the left or right when pressed during file parameter setting Up Down Moves the cursor one position to the up or down when pressed during file parameter setting Enter Press to accept current parameters as displayed on the screen Menu Press button to accept the current option displayed above the button E emm MA CIEZL DH CLASS WORLDWIDE ck User Section Chapter 1 e Basic Product Information 1 4 BLOCKS A variety of blocks are a
47. e returned to ESCO or to the distributer e Product return to Esco Esco will pay for the ONE WAY shipping costs to return the unit to Singapore e The unit should be returned whenever possible in its original packaging e If the serial number of this product is altered removed or defaced as to be illegible the warranty shall be null and void in its entirety This limited warranty does not cover e Damage resulting from shipping local deliveries or installation e lf your product was damaged in transit you must file a claim directly with the freight carrier e Products with missing or defaced serial numbers e Products for which Esco has not received payment e Problems that result from External causes such as accident abuse misuse problems with electrical power incorrect environmental conditions Servicing not authorized by Esco Failure to perform preventive maintenance Problems resulting from using accessories parts or components not supplied by Esco Damage by fire floods or acts of God Customer modifications to the product Factory installed customer specified equipment or accessories are warranted only to the extent guaranteed by the original manufacturer All users must register their equipment online at www escoglobal com warranty or complete the warranty registra tion form included with each product and fax to 65 6542 6920 ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT INCLUDING BUT NOT LIMITED TO ANY
48. e your equipment 4 2 2 1 Connect to Computer Connect the equipment to computer through RJ45 interface Cross Class B Connection RJ45 interface NG IH Computer Instrument Note There are two internet cables inside the box one is black and the other one is Gray If the PC only connects one unit directly please choose the gray color cable and the black color cable is for multiple units controlled via HUB Before use black cable to control up to 30 thermal cyclers please connect PC with every thermal cycler one by one and do the following setup Note PC requirements WinXP Win2000 operation system 4 2 2 2 Run Network Enable Administrator Broadcast button o x N Network Enabler Adsinistrator Configuration Eile Function Corihguration View Help 2232 eg Function Configuration 0 Network Enabler Module s Ei Network Enabler Admi ER Configuration 1 Configuration Mapping ISS Port Monitor AM IP Address Repost Function E Si Message Log 0 Monitor Log 0 Mow 2008 1 3 14 15 25 E emm MA CIZL DH CLASS WORLDWIDE 3O User Section Chapter Ae Computer Connection 4 2 2 3 Configuration Click the Broadcast button Se the software will search equipment automatically N Network Enabler Adajinistrator Configuration Die Function Configuation View Heb 22 2 ep Function
49. en setting Time as the hot lid will close automatically when the block reaches the set temperature And the set temperature will remain until the PCR process is being stopped Segment 1 Seg 1 Section 1 Cycle1 x From to Segment2 kk kk kk Cycle2 x From to r 6 Section 5 Cycle5 x From to Segment3 Seg 3 Sections and Segments in a File E WORLD CLASS WORLDWIDE 14 User Section Chapter 3 eRunning PCR Sample In this window press Digit amp Letter to access the file name FILE SAVE WINDOW Press Enter to move the cursor forwards one position Select User Hotlid off 2007 11 1 10 05 00 and File Name with the pop up key Click left button to delete characters and clicking the right hand button to move the cursor User ESCO Press CAPS to shift between upper and lower case letter and there File Name PCR1 will be a capital or small letters as shown on the top left of the Password KKK EK screen Press Main to return to the main menu Password check e Press Save to save the file When the file is saved the system will display File has been saved please continue another sae operation Press Enter to cancel the reminder If the file was used the system will remind you to rename Rename or Save e If the module is mixed block choose the tube type first pop up the following dialog box Select Tube Type 0 2m e If the current Control Mode
50. ewall settings Disable this network Bridge Connections Cem geg KE Create Shortcut Q Repair this connection Sen p Rename this connection Rename View status of this connection Properties A Change settings of this connection Other Places Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 37 Choose Internet Protocol TCP IP and click Properties A Local Area Connection Properties General Advanced Connect using Ep Intel R 825560 Gigabit Network Cc This connection uses the following items Mf QoS Packet Scheduler M Y AEGIS Protocol IEEE 802 1x 2 3 1 10 Description Transmission Control Protocol Intemet Protocol The default wide area network protocol that provides communication actos diverse interconnected nehworks Show icon in notification area when connected Nobly me when this connechon has limited or no connectrvity Set IP address the first 7 digitals 192 168 0 must be as same as the ones we set for Network Enabler Administrator and the last 3 digitals must be different from what we set for Network Enabler Administrator For example if we set IP address for thermal cycler as 192 168 0 245 in chapter 4 2 2 3 and we can set 192 168 0 145 here for PC Internet Protocol TCP IP Properties General You can get IP settings assigned automatically if your
51. fault User ESCO Dual block means two blocks Block A and B Press Shift to oper Default File PCR1 ate block A or B at the same time Control Mode Block For example to operate block A Self testing eeeeee The block that is currently active is diplayed on the top center of LO JM the screen The screen on the right shows the control is A B Press Shift to change the control Block x well The central area of the main screen displays the types of blocks Default User ESCO Default File PCR1 A amp B the default file default user control mode and sample Control Mode Block volume e Press File to enter file list screen Self testing eeeeee LL _ amp File USB Link System __ Run e Press System to enter system parameter setting screen e If the current file doesn t have password press Run to access File Run window If the current file has password press Run to pop up following dialog box Input password As same as single block press Back to go to the main window Press Accept to confirm the password e If the current Control Mode is Block Mode press Run to access File Run Interface If the current Control Mode is Tube Mode press Run The following dialogue box appears Select Sample Volume 050ul The user can input Sample Volume Unit Micro liter through the main menu Press Back to return to File List screen Press
52. gram Max Pro program Setup sam ome 2 InstallShield Wizard Complete Setup has finished installing Max Ps opeogram on your computer 1L O O M G Click Finish button to install the software successfully Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 2 7 4 2 NETWORK ENABLER SETUP MaxPro has configurable embedded internal network modules which provide the corresponding tools for Network Enabler setting up 4 2 1 Network Enabler Administrator installation Double click need_setup exe to install the software EP EE E E an AKE Ka a al TU Be Welcome to the Network Enabler Administrator Setup Wizard This will install Network Enabler Administrator 2 11 on your computer It is recommended that you close all other applications before continuing Click Next to continue or Cancel to exit Setup Click Next button 15 Setup Network Enabler Administrator Select Destination Location Where should Network Enabler Administrator be installed J Setup will install Network Enabler Administrator into the following folder To continue click Next If you would like to select a different folder click Browse C Program Files Network Enabler Administrato At least 2 5 MB of free disk space is required Choose installation Destination click Next button 15 Setup Network Enabler Administrator Select Additional
53. hift the cursor to next item This is followed by the Time 14 53 19 symbol ee Default User PCR1 Default File ESCO Press UP DOWN to scroll to the option you wish to select Other information can be accessed by pressing DIGIT amp LETTER Press RIGHT LEFT to change the cursor s position Control Mode Block Hotlid off Key Sound Yes e Press BACK to go back to the main menu Run End Sound Yes e Press SAVE to save the parameters Default e Press Default to restore the default files Standard PCR Gradient PCR Touch Down PCR etc Note After setting the parameters press SAVE or the parameters will not be saved 3 1 2 1 Date and Time Default time after power on 3 1 2 1 Default File and Default User Name and user of the default protocol after power on 3 1 2 3 Control Mode Default control model after power on including two optional modes called Block and tube Block mode Simple temperature control during cooling amp heating When compared to the cooling amp heating rate of the reaction In this mode the actual change of the reagent s temperature in the reaction tube is different from the actual set parameters The actual time for reagent to reach amp hold the set temperature is shorter than the set time Refer to the following figure showing the relation between block temperature and reaction temperature 100 97 94 91 88
54. hysical map sequencing and map expression Forensics Specimen analysis in the venue and HLA Dqalocus typing Oncology Pancreas cancer rectum cancer lung cancer thyroid gland cancer melanin cancer and leukemia Social and Colony Biology Genetic species research evolution research animal protection research ecology environmental sciences and experimental genetics Paleontology Biology Specimen analysis in archeology and museum Diagnosis of animal hereditary diseases and detection of plant pathogeny 3 Features of Swift MaxPro The Swift MaxPro Thermal Cycler is a multi function PCR amplification system with the following features A choice of Five of interchangeable blocks Advanced thermoelectric cooling Peltier technology with high quality Peltier units guaranteeing rapid stable and reproducible performance Fast ramp rates 4 C sec average heating Ramp rates can adjust according to different experimental conditions Multiple temperature control modes The Thermal Cycler s central processor can automatically distinguish the block type and automatically load the correct program User friendly large screen LCD and ergonomically designed interface Auto power off function Programs are resumed when the power comes on Hot lid pressure and temperature are adjustable USB interface allows data transfer between USB and equipment RJ45 standard interface allows up to 30 Swift
55. ics maximum ramp rate temperature accuracy and uniformity apply only within the temperature range 23 C 5 C General Specifications Swift MaxPro Thermal Cycler SWT MXP Sample Capacity 96 x 0 2ml 48 x 0 2ml 30 x 0 5ml 384 wells 48 x 0 2ml 48 x 0 2m 4 slides 0 2ml tubes Applied Consumables o e r T 384 micro plate Ge Ce S E ay 8 x 12 strips 4 x 12 strips Temperature Range 4C 99 C Max Heating Rate 4 0 C sec 2 8 C sec 2 8 C sec 4 0 C sec 1 8 C sec Max Cooling Rate 4 0 C sec 2 8 G7 sec 2 8 C sec 4 0 C sec 1 8 C sec Temperature Uniformity 0 3 C Temperature Accuracy 0 2 C Max Gradient 30 C 30 C Min Gradient 1 C 1 C Hot Lid Temp range 30 C 110 C Temp Control Mode Block mode or Tube mode Display 5 7 Graphical LCD Instrument Memory 100 protocols on board unlimited with use of USB memory stick and PC optionally password protected PC Interface RJ45 Dimensions W x D x H 340mm x 420mm x 280mm WxLxH Weight kg 10 Power Supply 100 240 V 50 60 Hz 600W Fuse 250V 8A 5 x 20 Warranty 3 years on main body 2 years on blocks 8 2 PERFORMANCE Maximum number of cycles 99 Maximum number of sections 5 Maximum segments within a cycle 16 Number of programs 99 Noise 59dBA Background Noise below 40dBA 8 3 EQUIPMENT SOFTWARE FUNCTION
56. is Block Mode press RUN to access the File Run Window If the current Control Mode is Tube Mode press RUN The following dialogue box will appear Select Sample Volume 050ul The user can input Tube type and Sample Volume Unit Micro liter the same way as in the main interface e Press BACK to return to the File List window e Press RUN to enter into file run Window Note Set password as 0 9 number characters While the file is running NOW RUNNING will flash continuously FILE RUN WINDOW When the run ends the system will specify FILE RUN OVER Press STOP The message CONFIRM STOP RUNNING appears Hotlid oft 2007 11 1 10 05 00 e Select STOP or START e Press PAUSE The message NOW PAUSE RUNNING appears Select START to continue If you don t make a selection within 100 seconds the run will continue automatically e Press VIEW FILE to view edited PCR programs 1c 95 0 2c 55 0 3c 72 0 01 00 02 00 01 45 e Press SKIP to stop the current temperature and to run the next File Name PCR User ESCO Now Running eee 1c Temp 00 0 Time 00 00 Cycle 01 of 10 Total Time 00h 56m Remaining Time 00h 56m Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 3 e Running PCR Sample 15 3 1 2 How to set system parameters SYSTEM PARAMETER SETTING WINDOW In the main window press SYSTEM to enter the system parameter Hotlid off 2007 11 1 10 05 00 set interface Date 03 10 22 Press ENTER to s
57. ity Data Bits Stop Bits Click OK to confirm Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 33 Click COM MAPPING in the function list N Network Enabler Administrator COM Mapping Die Function COM Mapping View Help Add Target DD OS A equipment Furjction COM Mapping 0 COM PY Wetwtrk Enabler Admi No 1 Model IPAddress Pot COM Pot Mode Parameter Kay ste Remove Target Lem eguipment ngeker Apply change 2008 1 3 14 26 33 Found Network Enabler Module s 1 2008 1 3 14 35 18 Configuration Changed NE 4110S 00 90 ES 10QE287 2008 1 3 14 35 39 Configuration Changed NE 41105 00 30 E amp 10E287 2008 1 3 14 35 49 Configuration Changed NE 41105 00 30 E810E287 2008 1 3 14 37 22 Configuration Changed NE 41105 00 90 E810E287 N Network Enabler Administrator CON Wapping Fie Function COM Mapping View Help Dip SA Function COM Mapping 1 COM NE 41105 192 168 0 245 COM Hi Pestormance FIFO Ena 9600 None 8 1 Nor A IP Address Repost 2008 1 3 14 26 33 Found Network Enabler Module s 1 20081 3 14 35 18 Configuration Changed NE 41105 00 90 E8 10 E2 87 2008 1 3 14 35 39 Configuration Changed NE 41105 00 90 E8 10 E 287 2008 1 3 14 35 49 Configuration Changed NE 41105 00 90 E8 10 E2 B7 2008 1 3 14 37 22 Configuration Changed NE 41105 00 90 E8 10 E287 E emm MA CIZL DH CLASS WORLDWIDE 34 User Section Chapter Ae Computer
58. l SWT MXP SWT MP BLC1 SWT MXP SWT MP BLC2 SWT MXP SWT MP BLC3 SWT MXP SWT MP BLC4 SWT MXP SWT MP BLC5 in accordance with the following directives e 2006 95 EEC The Low Voltage Directive and its amending directives e 89 336 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed to comply with the requirements of the following Harmonized Standard e Low Voltage EN 61010 2 010 2003 e EMC EN 61326 1 2006 More information may be obtained from Esco s authorized distributors located within the EU A list of these parties and their necessary contact information is available on request from Esco Lim Lay Yew Chief Executive Officer Applicable only to 100V 240V AC 50 Hz 60 Hz thermal cycler E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual 1 About Esco Swift MaxPro PCR Thermal Cycler KI ABOUT ESCO SWIFT MAXPRO PCR THERMAL CYCLER Brief Introduction of PCR Technique PCR Polymerase Chain Reaction or cell free molecule cloning is a technique for amplifying nucleic acids in vitro emulating natural DNA replication process The PCR technique using two DNA strands to be amplified as template and a pair of synthetic oligonucleotides as primers rapidly reproduces the specific DNA pieces under the catalysis of a thermostable DNA polymerase Because of its simplicity rapidity specificity and sensitivity PCR h
59. l mode Tube D Default user I Default file KH Save ESO CD OOL CLASS WORLDWIDE 44 User Section Chapter 4 Computer Connection 4 3 3 User menu Create a new user 1 Choose Create New User under User menu pop up create user dialog box 2 Enter your user name click OK button Create User Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers J OK x Cancel Change the user name 1 Choose Change User Name under the User menu pop up the Change User Name dialog box 2 Enter user name click the OK button Change User Name Do you want to change user name of Computer s 123 Enter new user name The user name can contain only letters and numbers Jf OK X Cancel Note When the user s file is opened the user name can not be changed Copy User 1 Choose the user name under equipment 2 Choose Copy User under User menu Paste user 1 Choose the name of the equipment or any user name under equipment 2 Choose Paste User menu under User menu Note 7 f the user name already exists and its file is opened the user can not be pasted 2 Only can paste between the local computer and equipments Delete user 1 Choose the user name need to be deleted 2 Choose Delete User under User menu Note When the user s file is opened the user can not be deleted Swift MaxPro Thermal Cyclers e User
60. ler should be placed in a low humidity dust free and well ventilated room and as far away from any source of water or humidity The instrument should not be located directly under an air conditioner 2 2 2 Normal operating conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power supply 100V 240V 600W 50 60Hz E emm MA CIEZL DH CLASS WORLDWIDE amp User Section Chapter 2 Installation 2 2 3 Transportation and storage conditions Ambient temperature 20 c 55 c Relative humidity lt 80 2 2 4 What to avoid 1 Cover or obstruct the ventilation openings of the thermal cycler 2 Near to other instruments on the same bench or other heating generating equipment 3 Damp areas 4 Environments where there is oil mist 5 Locations subject to heat sources like sunlight ovens or central heating equipment 2 3 INSTALLING YOUR INSTRUMENT 2 3 1 Installation of the Block Install and Remove the block as follows 1 Installation e Place the block Gr into the housing well 0 and push it slowly forwards until a connection between the block socket and the main unit s connection plug ED is achieved e Press the locking handle CN downwards the block will move forward automatically until you hear the click sound which means the block is installed successfully e Do not force 2 Removing the Block e Turn off the power supply e Uplift the locking handle CH the block will
61. n order to make sure our products are outstanding in performance For further details of these systems visit www cyclertest com E emm MA CIEZL DH CLASS WORLDWIDE Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 2 Installation 7 CHAPTER 2 INSTALLATION 2 1 UNPACKING YOUR INSTRUMENT 2 1 1 Inspecting the instrument Inspecting the Swift MaxPro Thermal Cycler and all other supplied parts for any damage that may have occurred during shipment If there has been any damage during transit notify the carrier and distributor immediately Note Save the shipping container and all packing materials in case it becomes necessary to reship the instrument 2 1 2 Packing contents The following items are included along with your manual 1 Swift MaxPro thermal cycler Power cable USB cable RJ45 cable Fuse Compact Disk Performance testing report O So M a A wW N Packing list 2 1 3 Additional Part You can order other block from Esco Order parts Order Code Swift MaxPro Cycler Main Body 100V 240V SWT MXP Swift MaxPro Block 1 96x0 2ml Gradient SWT MXP BLC 1 Swift MaxPro Block 2 30x0 5ml 48x0 2ml SWT MXP BLC 2 Swift MaxPro Block 3 384 well plate Gradient SWT MXP BLC 3 Swift MaxPro Block 4 48 x 0 2ml x2 SWT MXP BLC 4 Swift MaxPro Block 5 4 n situ slides SWT MXP BLC 5 2 2 INSTALLATION LOCATION REQUIREMENT 2 2 1 Choose a location The Thermal Cyc
62. network supports this capabdity Otherwise you need to ask your network administrator for the appropriate IP settings Obtan an IP address automatically Use the following IP address IP address Subnet mask Default gateway 182 Use the following DNS server addresses Prefered DNS server 203 Akemate DNS server 203 E emm MA CIEZL DH CLASS WORLDWIDE 38 User Section Chapter Ae Computer Connection 4 2 4 Add thermal cycler IP address in MaxManager PC software MaxManger Keep the Network Enabler Administrator open double click the the following fig PC software icon system will go to Swift MaxPro And will enter the main interface automatically as follows User File Tools Help 188883288 R2R3008eR2000 TT B ale 2 Sa I CORT Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection K Click Tools and then choose Server Setting Click Add and key in the IP address of the thermal cycler which we set in Chapter 4 2 2 3 As the Fig below 192 168 0 245 It have been saved successfully But it won t work untill you restart this program Le E emm MA CIEZL DH CLASS WORLDWIDE 4O User Section Chapter Ae Computer Connection Close the MaxManager PC software completely and open it again Before enter the main interface you will see the fig as below waitto be accepted
63. nly numbers Enter new password again Use empty password Jf OK x Cancel Note Opened file s password can not be changed Save file Click Save File after modification Import file 1 Choose the local computer user 2 Choose Export File under the File menu 3 Choose the file and click Open button Note When the file name exists and it is opened can not import the file E WORLD CLASS WORLDWIDE 418 User Section Chapter 4 Computer Connection Export File 1 Choose the file need to export 2 Choose Export File under File menu 3 Choose the export target path click OK button Note Opened file can not be exported 4 3 5 Tool menu Connect E button Choose the equipment click Connect under Tools menu or Note One equipment can only be connected to one computer at the same time Disconnect ZS Choose the equipment click the Disconnect under Tools menu or button to disconnect the equipment from the computer Grad Temp 1 Choose Grads Temp Enter the step temperature and gradient range 2 3 Choose the block type 8x12 96well or 16x24 384well 4 Click Calc Button to calculate the gradient temperature for each column ra User File Tools Help R225 28 LLAR TT JIR Computer Grad Temp Zu B 34 Temp 30 Gradient 24 G 8X12 16X24 wi Calc Colder gt Hotter 06066006 600 000000 00000 000000 0600 000000 00
64. nnecting the Power Cord Insert the plug properly to ensure good contact between the plug and socket When removing the plug pull on the plug and not the cord f Environmental Considerations The Thermal Cycler should be placed in a low humidity dust free and well ventilated room and as far away from any source of water or humidity The instrument should not be located directly under an air conditioner Never cover or obstruct the ventilation openings of the Thermal Cycle These are designed for ventilation and prevent the instruments interior from becoming too hot When a single instrument is installed the minimum distance between its openings and the nearest object should be 50cm When two or more instruments are running at the same time the minimum distance should be 100 cm Do not place the instrument on a soft surface Excessively high temperatures will result in poor performance or failure of the Thermal Cycler The instrument should be protected against any kind of heat sources like sunlight ovens or central heating equipment Do not place in direct sunlight such as ion a window sill If the Thermal Cycler is not used for a long time it is recommended to disconnect the power line and to cover the device with a piece of soft cloth or plastic to prevent dust from entering WORLD CLASS WORLDWIDE vi Introduction 3 LIMITATION OF LIABILITY The disposal and or emission of substances used in connection with this equipme
65. nt may be governed by various local regulations Familiarization and compliance with any such regulation are the sole responsibility of the users ESCO s liability is limited with respect to user compliance with such regulations 4 EUROPEAN UNION DIRECTIVE ON WEEE AND RoHS The European Union has issued two directives e Directive 2002 96 EC on Waste Electrical and Electronic Equipment WEEE This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol ESCO sells products through distributors throughout Europe Contact your local ESCO distributor for recycling disposal Please note that for contaminated devices example biological safety cabinets it is the responsibility of the user to ensure the product is decontaminated prior to disposal and to provide Certificate of Decontamination to the ESCO distributor disposal agency e Directive 2002 95 EC on Restriction on the use of Hazardous Substances RoHS With respect to the directive on ROHS please note that this cabinet falls under category 8 medical devices and category 9 monitoring and control instruments and is therefore exempted from requirement to comply with the provisions of this directive 5 SYMBOLS Important safety messages are provided in this operating manual Always read and obey all safety messages Failure to comply with the following warnings and cautions c
66. ntrol Mode is Tube Mode press Run The dialogue box below will appear Select Sample Volume 050ul The user can input Sample Volume Unit Micro liter in the same way as through the main menu Press Back to go back to File List menu Press Run to enter into file run menu E emm MA CIEZL DH CLASS WORLDWIDE 20O User Section Chapter 3 eRunning PCR Sample 3 3 1 How to edit a PCR program FILE LIST WINDOW Press File in the main menu to access the file list menu Hot lid off 2007 11 1 10 05 00 There are 3 files listed as shown in the picture When the bar is flashing in the User Column select the users with the User Save Time PCR1 2003 11 1 At this point the File Name Column will display the rela geg 3 PCR2 2003 11 1 ih PCR3 2003 11 1 tive files of the selected user Shift to the File Name Column with the flashing bar then select relative user s file with the pop up key e Press Edit to edit the highlighted file 1 e Press New File to edit a new file e Press Delete The alarm will sound Confirm delete file Select Delete or Back e Press Back to return the main menu e If the current Control Mode is Block Mode press Run to enter into File Run menu If the selected Control Mode is Tube Mode press Run and the following dialogue box will appear Select Sample Volume 050ul The user can input Sample Volume Unit Micro liter in the same way as shown in the main menu Pres
67. ompt If the file was Password check used the system will remind you to rename Rename or Save Password KANAN e lfthecurrent Mode is Block Mode press Run to access file run window If the current Mode is Tube Mode press Run and the following dialog box will appear Select Sample Volume 050ul FILE RUN WINDOW The user can input Sample Volume Unit Micro liter in the same way as before Through the main menu Press Back to return to _Hotlid off 2007 11 1 10 05 00 File List menu Press Run to access the file run menu 100 75 50 25 e Press Stop and a Confirm stop running prompt will appear 0 Select Stop or Start 1c 95 0 2c 55 0 3c 72 0 4c 04 0 e Press Pause the Now pause running prompt will appear Select 01 00 02 00 01 45 Start to continue If you don t select within 100 seconds the File Name PCR When the file is running eee at Now Running will flash continu ously When the run is over the system will prompt File run over system will automatically continue running User ESCO Now Running eee 1c Temp 00 0 Time 00 00 Cycle 01 of 10 e Press View File to view the edited PCR programs Total Time 00h 56m Remaining Time 00h 56m e Press Skip to stop the current temperature and to run the next 1 2 3 4 5 6 7 8 9 10 11 12 950 951 952 954 957 959 961 964 966 968 969 970 ESCO WORLD CLASS WORLDWIDE User
68. on Chapter 3 eRunning PCR Sample e Press Back to go back to USB user list window USB USER LIST e Press up down cursor to choose the file which will be imported Hotlid off 2007 11 1 10 05 00 from USB memory stick File 1 e If the files are more than 30 press left right cursor to previous File 2 next page e Press Copy to input file from USB memory stick e If copy the file from USB successfully the following dialog box will appear Press Left Right Key to Previos Next Page File Saved Back JI en Otherwise display File input error 3 4 3 Export file to USB flash memory e Press Back to go back the USB link window INSTRUMENT USER LIST e Press up down cursor to choose the user Hotlid off 2007 11 1 10 05 00 e lf the users are more than 30 press left right cursor to previous Local next page User 1 User 2 e Press View key to display Instrument file list Press Left Right Key to Previos Next Page Back JI 1 ven e Press Back to go back to instrument user list window INSTRUMENT FILE LIST e Press up down cursor to choose the file which will be exported Hotlid off 2007 11 1 10 05 00 e Ifthe files are more than 30 press left right cursor to previous User 1 next page User 2 e fsave the file to USB memory stick successfully display the following dialog box File Saved Press Left Right Key to Previos Nex
69. s Back to return to File List menu Press Run to access the file run menu Note The sign at the top of User and File shows that the current run file is the default file This can be modified in the parameter setting menu In this window move the cursor by pressing Up Down and FILE EDIT WINDOW Right Left The cursor position will be displayed in white on a black background Press Digit amp Letter to modify parameter settings Press Function to change but 100 The zero cannot be modified Should you need to return to the main menu press Save then press Main e Press Seg To access the segment edit interface The 0 2c550 3c72 0 4c 040 set temperature Temp duration Time Ramp rate j e 02 00 Ramp temperature increment of each cycler Temp and time increment of every cycler Time can be set Temp C Time Ramp C s Temp Time p Delet to deleteth t t 95 0 01 00 0 0 0 00 ress Delete to delete the current segment 55 0 02 00 0 0 0 00 Sp Cader eee ee g 72 0 01 45 0 0 0 00 ress Cycle to access the cycle edit menu where you 04 0 aes 400 40 00 can set cycle numbers and initial cycle segment The fig Cycle1 x10 From 01 to 03 ure shows a cycle from the first to the third segment There are totally 10 cycles Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 3 e Running PCR Sample 21 e Press Cycle repeatedly to add sections Max 5 Press
70. ser and Service Manual 3 4 HOW TO IMPORT AND EKPORT THE PCR PROGRAM 3 4 1 Data transfer by USB memory stick Press Usb link in the main window to access the USB Link Window If USB is inserted information will appear as right fig Press Back to go back to the main Window Press Input to choose file from USB USB user list will be displayed Press Output to choose file which will be copied to USB local file user list will be displayed If no USB memory stick is inserted information will appear as right fig Press Back to go back to the main window 3 4 2 Import file from USB memory stick Press Back to go back to the USB link window Press up down cursor to choose user If the users are more than 30 press left right cursor to previous next page Press View to display USB file list User Section Chapter 3 e Running PCR Sample 23 USB LINK WINDOW Hotlid off 2007 11 1 10 05 00 The Instrument has Connected UsbDisk Please Choose Input or Output File Press F1 to Back Press F4 to Input File Press F5 to Output File Back Jl Input Output USB LINK ERROR WINDOW Hotlid off 2007 11 1 10 05 00 No disk connection Press F1 to Back Back JI II USB USER LIST Hotlid off 2007 11 1 10 05 00 File 1 File 2 Press Left Right Key to Previos Next Page Back JI 1 en E emm MA CIEZL DH CLASS WORLDWIDE 24 User Secti
71. t access modify delete or run a PCR amplification program and how to set the operating pa rameters for each block Immediately turn the power off and contact the distributor if any abnormal sounds or display appear after power on or if the alarm sounds during self testing Note f the number of samples is less than the number of wells in the block the reaction tubes should be evenly distributed over the block This ensures that the heated lid will exert even pressure on the the sample tubes Turn the knob counter clockwise before closing the lid Note that the knob can be tightened by turning it clockwise and can be loosened by turning counter clockwise After closing the cover but before power on turn the knob clockwise until you hear the click sound If hear click sound at the beginning of turning the knob clockwise and counter clockwise please press the unlocking device and turn the knob counter clockwise for two rounds to loose the knob DO NOT OVERTIGHTEN HAND TIGHTEN ONLY 3 1 HOW TO RUN A SINGLE BLOCK PCR PROGRAM Main Window Switch the power on The Thermal Cycler will beep twice Hotlid off 2007 11 1 10 05 00 The screen will display Self testing and the instrument will self test Self testing requires about 1 2 minutes If the self testing ESCO MICRO Pte Ltd i Version 2004 1 2 is successful the display on the right will appear Block x Default User ESCO Default File PCR1 Control Mode Block File
72. t Page Otherwise display O I Do File input error e Press View key to display USB file list Note USB users are up to 500 files for each user are up to 2000 Every user has separate folder under usb gt Esco Don t insert and then immediately pull out USB memory stick If do so in order to protect both instrument and USB memory stick the USB link function will not available until the next time when instrument power on The operations for dual block are as same as single block and the USB link function is only available when main window is displayed Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 4 e Computer Connection 25 CHAPTER 4 COMPUTER CONNECTION 4 1 SOFTWARE INSTALLATION Double click Setup exe to install MaxPro PC software which can control up to 30 sets of MaxPro Thermal Cyclers simultaneously through one computer Max Pro program Setup Welcome to the InstallShield Wizard for Max Pro program The InstallShield Wizard will install Max Pro peogram on yout computer To continue chok Next Click Next Button Max Pro program Setup License Agreement Please read the following cence agreement carefully Press the PAGE DOWH key bo pee the rest of the agneenent Bolma License Authority License ESCO hereby gullhronze the sppkeation met of Ihis software bo you walk regard to poui quarardy lo our Sampang No amendment
73. to the main window interface Lot 4 ESCO1 PCR1 2003 11 1 11 1 e If the current file doesn t have password press Run to ee PCR2 2003 11 1 access File Run window and tube type selection window If ESCO3 PCR3 2003 11 1 the current file has password press Run to pop up follow Edit NewFile Delete Back Run ing dialog box Input password e Ifthe module is mixed block choose the tube type first pop up the following dialog box Select Tube Type 0 2m Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 3 e Running PCR Sample 13 e If the current Control Mode is Block Mode press Run to enter into File Run window If the current Control Mode is Tube Mode press Run and a dialog box will appear as follows Select Sample Volume 050ul The user can input Password Tube type and Sample Volume Unit Micro liter in the same way as before Press Back to return to File List window Press Run to access the file run window Note The sign at the top of User and File shows that the current run file is the default file This can be modified in the parameter setting window In this window you can edit sections and segments of the file FILE EDIT WINDOW A File consists of sections and segments The sections contain Hotlid off 11 1 10 05 many segments which you can set the cycler to run refer to eres bees the figure
74. uipment to 4 2 3 PC IP address setup Click Start choose My network places the following windows will pop up Pie Edt View Favorites Tools Help Qe CQ JI muh Kries DO Md my Network Places Network Tasks w Add a network place W view network connections Set up a wireless network For a home or small office lt j Search Active Directory Show icons for networked UPnP devices Other Places CG Desktop feb Entire Network My Computer EJ My Documents Kb Printers and Faxes E emm MA CIEZL DH CLASS WORLDWIDE 36 User Section Chapter Ae Computer Connection click View Network Connections in the left side A window will pop up as follows s Network Connections File Edit View Favorites Tool Advanced Help Q DS Ki JO search Ki Folders HO Address MO Network Connections A LAN or High Speed Internet Network Tasks Local 4rea Connection sa Create a new Connected connection fh Intel R 825660M Gigabit Net ga Change Windows Firewall settings See Also i Network Troubleshooter Other Places Dr Control Panel Right Click the icon as below and click Properties a Network Connections File Edit View Favorites 0 Back Address Network Connections A LAN or High Speed Internet Network Tasks CES COU Dicabl Create a new J 0 SS od i e connection R 625660M Status Change Windows Repair Fir
75. umber 1 Port s Selected 1st port is Port 1 Basic Settings Advanced Settings Serial Parameters Baud Rate Parity None v Data Bits 8 Stop Bits 1 Flow Control None X Click OK button apply configuration ESO CD OOL CLASS WORLDWIDE 56 Chapter 5 Equipment Software Upgrade Run upgrade exe Press Open choose the upgrade file bin in the computer Press Start the computer start to upgrade the software of instrument LCD displays Upgrade begin During software upgrade LCD displays Configuring Mode eee When the software upgrade is complete LCD displays Upgrade finished eee Se ei SY SS Equipment software upgrade is finished 10 Click Remove Target equipment to remove the equipment mapping 11 Restore configuration Open Network Enable Administrator to choose configuration function Network Enabler Administrator Configuration File Function Configurabon View Help 222 SA Function Configuration 0 Network Enabler Module s Bal Network Enabler Adma Configuration Ty Configuration Genie ES ert Monitor AN IP Address Repot New 2008 1 3 14 15 25 Swift MaxPro Thermal Cyclers e User and Service Manual Chapter 5 e Equipment Software Upgrade 57 Double click the searched IP address choose Operation Mode and click setting button Configuration KE Information Auto Waring IP Address Report Password Digital 10 Senal CMD Model
76. vailable for different requirements SWT MXP BLC 1 96x0 2ml Gradient block kd U Standard 0 2ml Centrifuge Tube SWT MXP BLC 2 30x0 5ml 48x0 2ml Standard 0 2ml Centrifuge Tube Standard 0 5m Centrifuge Tube SWT MXP BLC 3 384 well plate Gradient block 384 well plate SWT MXP BLC 4 48x0 2ml 48x0 2ml SWT MXP BLC 5 4 n Situ Slides In situ slides Swift MaxPro Thermal Cyclers e User and Service Manual User Section Chapter 1 e Basic Product Information 5 1 5 TESTING 1 5 1 Basic testing Before being shipped each individual unit is extensively tested for performance and safety and delivered with a detailed test report Testing performed at our factory includes Appearance and External identifier Electrical safety e Protected grounding resistance e Leakage current to ground e Leakage current to plastic shell e Dielectric intensity e Insulation Anti jamming performance Function testing Performance testing e Temperature accuracy e Temperature uncertainty e Block temperature uniformity e Temperature control range e Maximum Heating and cooling rate e Hot lid working temperature range USB interface AND RJ45 interface 1 5 2 Additional testing Our cyclers are also tested by The Cyclertest Drifton system which is next to the MTAS certification the ideal tool to serve as a periodic validation instrument for PCR Tests i

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