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User's Manual - Fisher UK Extranet

1. should be performed frequently per your established Ki NOTE Main chamber pressure and smoke removal tests quality system to ensure safe performance Summary of Performance Testing Results Labconco s test results show that the XPert Weigh Box meets all the performance criteria when operated at the following estimated airflow volumes 61 CFM or less for weighing with an analytical balance or handling powders and 61 CFM or above for safe air clean up The XPert Weigh Box has been tested to the point of insufficient performance In each case the lack of performance occurred at airflow volumes below Labconco s minimum recommended airflow volume for the specific operation Contact Labconco or visit www labconco com for complete performance test data The following provides a brief summary of the findings e Smoke removal was tested at minimum 13 CFM to maximum 100 CFM airflow volumes The time required to completely purge the main chamber of smoke varied from approximately 75 seconds at 100 CFM to 420 seconds at 13 CFM e During tracer gas containment tests with the glove box vented to the outside no gas was detected within the operator s breathing zone Perimeter scans were also conducted during tracer gas testing of the XPert Weigh Box Under normal laboratory conditions the tracer gas levels detected during perimeter scan testing were below ASHRAE 110 permissible limits 0 10 PPM average or less and achieved Labconco permissible
2. 1640601 9 size Butyl Gloves 3C 1640602 9 size Hypalon Gloves 3D lpr 1640500 8 Y size Neoprene Gloves 1640501 8 Y size Butyl Gloves 1640502 8 4 size Hypalon Gloves Zea 1640000 O Rings 2e 1965600 Clamp O Ring Gloves 2e e Description Framed Glass Window standard Framed Acrylic Window optional Receptacle 115V Duplex Receptacle 230V Duplex Sealed Duplex 115V Complete Sealed Duplex 230V Complete 1A IB A B D 2E A 2 2 2 2 3 Go W Go a a 1952500 Pressure Differential Gauge 1307000 Switch Rocker 2 Position 6 3704402 Blower Speed Control 115V a a 1931500 Knob Speed Control 5229400 Zea 1890302 lea Extension Speed Control Set Screw Extension 8 32 x 12 Blower Speed Control 230V HEPA Filter 99 99 ULPA Filter 99 999 Bag In Bag Out Kit Nut Keps 10 24 Motorized Impeller 115V also used on 100V units Motorized Impeller 230V Fluorescent Bulb 8 Circleline FC8T9 CW 22 Watt 4 Pin Ballast 115V 60 Hz Ballast 230V 50 Hz also used on 100V 50 Hz Ballast 230V 60 Hz also used on 100V 60 Hz lea 3704403 5225105 5225106 5241700 1905617 5227300 5227301 5222700 6 7 7 7 7 8 9 5222800 5222801 5222802 3E 3F 4 5 A C 6E A B C D A 8B A C 9 lea lea dea lea EF EF lea lea lea 5 16 lea 5226500 1327204 1327205 1327200 5226900 5226901 5234400 5235100 5234600 5211500 US Power Strip 115V 4 Posi
3. User s Manual XPert Weigh Boxes Models Voltage Frequency 5220300 115V 60 Hz 5220320 230V 50 Hz 5220321 230V 60 Hz 5220330 100V 50 Hz 5220331 100V 60 Hz To receive important product updates complete your product registration card online at register labconco com Protecting your _ laboratory environment lA 7 LABCONCO Labconco Corporation 8811 Prospect Avenue Kansas City MO 64132 2696 800 821 5525 816 333 8811 FAX 816 363 0130 E MAIL labconco labconco com Belgique Belgi t l 056 260 260 fax 056 260 270 be fisher thermofisher com www be fishersci com Espana tfno 91 380 67 10 fax 913808502 es fisher thermofisher com www es fishersci com m m en France t l 03 8867 53 20 fax 03 8867 11 68 fr commande thermofisher com www fr fishersci com Fisher Scientific Italia tel 02953 28 258 fax 02 953 27 374 it fisher thermofisher com www it fishersci com Part di Thermo Fisher S lentific Netherlands tel 020487 7000 fax 020487 70 70 nl info thermofisher com www nl fishersci com Portugal tel 21425 3350 fax 21 425 33 51 pt fisher thermofisher com www pt fishersci com Suisse Switzerland t l 05661841 11 fax 056618 41 41 info chOthermofisher com www ch fishersci com Copyright 2007 Labconco Corporation All rights reserved The information contained in this manual and the accompanying products are copyrighted and all rights reserved by Labconco Corporation Labconco Corporation reserves the right to make p
4. classification and larger than the considered sizes shown below concentration limits are number N calculated in accordance with 3 2 of Standard 14644 1 Olpm O2um 03m 0O5pm lpm Sum pap map y AA g em IRMA IE ARE AE AO ee A E IE E E Woar eee ee E II RU Budd MR Ci eee ee IE ML Ml STA a w AAA a ISO Class 9 293 000 Table 2 1 ISO Classification Number N Additionally the motorized impeller can also be used in a full recirculation mode providing the operator with some control of the atmosphere chemistry within the glove box See Chapter 7 for the Recirculation Conversion Kit The airflow chart below represents typical operational data including air changes per minute exhaust volume noise levels main chamber static pressures and total system static pressures measured at the blower intake suction side Exhaust volumes are reduced by 17 for products operating on 100V 60 Hz and reduced 34 for 100V 50 Hz products Air Changes Exhaust Newco Pressure Static Pressure Total System Dial Setting per Minute Volume dB A Inside Glove Box Static Pressure acm inches of water inches of water Min Dial 20 Dial 40 Dial 50 Dial 60 Dial 80 Dial Max 100 Dial Dial OFF XPERT WEIGH BOX Static Pressure Test Results Static Pressure Inside Glove Box Total Static Pressure at Blower Intake Suction Side Negative Static Pressure Inches of Water Column yo 0 5072 0 3435
5. as a Time Weighted Average The exposure limit for that chemical for up to a 10 hour workday 40 hours a week Expressed in units of parts per million or milligrams per cubic meter Odor Threshold The value in parts per million or milligrams per cubic meter for which one might expect to smell a chemical s presence in the air This value is very subjective and detection will vary with the sensitivity of one s nose The period of time until the odor threshold is reached in the exhaust stream can be estimated from Labconco exclusive computerized filter modeling program Contact Labconco on carbon filter life for specific applications See Chapter 6 Saturation Level or Time There 1s a limit to the amount of chemical that can be adsorbed by activated carbon or neutralized by chemically treated carbon Once the capacity of the carbon is reached it is considered to be saturated and will adsorb or neutralize no further material the outlet concentration of the chemical will equal the inlet concentration from that point until the filter is replaced Note that the capacity of activated carbon is not a constant but varies with the inlet concentration Labconco Technical Specialists can determine with the computerized carbon modeling program the estimated time saturation for a particular chemical When using a HEPA filter in all filtered glove boxes or in combination with a carbon filter the speed control will need to be increased to allow f
6. be supported independently of the glove box superstructure or damage may occur The exhaust system should be installed by a qualified HVAC contractor YJ t The XPert Weigh Box has been manufactured to exhaust into the laboratory in its standard configuration For exhausting to the outside of the building see Figure 3 2 a Thimble Exhaust Connection should be ordered see Chapter 7 To remove chemical fumes and odors not trapped by the HEPA filter the glove box can be exhausted to the outside or connected to an accessory FilterMate Portable Exhauster with carbon filters see Figure 3 2 See Chapter 7 for accessories including Thimble Exhaust Connection FilterMate Portable Exhauster Positive Pressure Conversion Kit Recirucation Kit Hard Duct Connection Consult Labconco Customer Service if you require help sizing a remote blower for the exhaust volume and the duct work system static pressure loss listed in Chapter 2 Chapter 3 Getting Started To ensure compatibility the selected exhaust duct material should match the enclosure procedures and chemical applications w F me o af Es T7 EXHAUST HEPA i O y 526 m e 8 0 NOMINAL GLOVE PORTS Figure 3 2 Exhaust Connection to Outside All dimensions in inches Product Service 1 800 522 7658 11 Chapter 3 Getting Started POSITIVE PRESSURE AIRFLOW DIRECTION POSITIVE PRESSURE HOSE BLOWER CONNECTION EXHAU
7. can be configured to add a waste chute from which a waste bag can hang Waste bags and bands can be ordered from your laboratory dealer The minimum waste bag size 1s 8 5 wide Exterior Transfer Chamber Shelf Part 5245900 A powder coated steel shelf 10 7 wide x 15 1 deep can be attached to the top of the transfer chamber The exterior transfer chamber shelf maximizes work area useful for printers laboratory supplies and notebooks Chapter 8 Troubleshooting Refer to the following table if the XPert filtered glove box fails to operate properly If the suggested corrective actions do not solve your problem contact Labconco for additional assistance PROBLEM CAUSE CORRECTIVE ACTION E ee ee A A outlet service Ground Fault Interrupter disconnected unit defective blower is defective Chapter 6 operate outlet Circuit breaker s tripped Reset or replace circuit breaker properly disconnected Po Defective lamp Replace lamp Fo Light switch is defective Replace light switch Defective electronic Replace ballast ballast Appendix A Replacement Parts The components that are available for the XPert filtered glove box are listed The parts shown are the most commonly requested If other parts are required please contact Product Service Item Part Number 5232200 5236100 1230700 1283900 C 5232900 5232901 5233400 Wallplate Duplex Gray 1640600 9 Y size Neoprene Gloves standard
8. for indirect consequential or special damages of any kind This statement may be altered by a specific published amendment No individual has authorization to alter the provisions of this warranty policy or its amendments Lamps and filters are not covered by this warranty Damage due to corrosion or accidental breakage is not covered Returned or Damaged Goods Do not return goods without the prior authorization from Labconco Unauthorized returns will not be accepted If your shipment was damaged in transit you must file a claim directly with the freight carrier Labconco Corporation and its dealers are not responsible for shipping damages The United States Interstate Commerce Commission rules require that claims be filed with the delivery carrier within fifteen 15 days of delivery Limitation of Liability The disposal and or emission of substances used in connection with this equipment may be governed by various federal state or local regulations All users of this equipment are required to become familiar with any regulations that apply in the user s area concerning the dumping of waste materials in or upon water land or air and to comply with such regulations Labconco Corporation is held harmless with respect to user s compliance with such regulations Contacting Labconco Corporation If you have questions that are not addressed in this manual or if you need technical assistance contact Labconco s Customer Service Depa
9. in front of the ionizer The time for neutralization increases as the distance from the ionizer increases The time required for sufficient electrostatic decay will also vary depending upon user application moisture levels within the supply air and the level of cleanliness within the glove box interior 46 Product Service 1 800 522 7658 Test Point Locations and Voltage Levels Metal Metal Metal Back Left Right Interior Housing Housing Transfer Interior Interior Interior Lamp Exhaust Chamber Surface Surface Surface Window Electrostatic with Glove Box Blower amp Ionizer Fan OFF 100 CFM Blower amp Ionizer Fan ON After 1 Hour 100 CFM Blower amp Ionizer Fan ON After 2 Hours Static Electricity Test Results VOLTS on the Glove Box Neutralization is best between 100V The mini zero volt ionizer s neutralization time will be shortest when objects are approximately 12 to 36 directly in front of the 1onizer and will increase as the distance from the ionizer increases Set the fan speed switch on the side of the ionizer to the LOW or HIGH position Higher airflow will result in faster neutralization rates Position the ionizer so that the maximum airflow is directed at the items or area to be neutralized The LED will turn on during power up and remain GREEN during normal operation The ionizer has a grounding jack and must have a good earth ground to maintain proper balance Always plug the AC power adaptor into the
10. 1 Includes the valve O ring and nut to supply the main chamber or the transfer chamber to purge vacuum fill gas or connect a drying train One each Identifier Label Set for valves Part 5236600 is ordered separately 10 Vacuum Aspirator Kit Includes the valve hose canister and vacuum pump for evacuating fluids around the work surface 5241800 Vacuum Aspirator Kit 115V 5241801 Vacuum Aspirator Kit 230V 11 Electrical Power Strips These convenience electrical power strips are available in US and international versions and plug into the interior duplex of the glove box The 115V 4 position 5A US Power Strip is already included on 115V XPert Weigh Boxes Descriptions 5211500 US Power Strip 115V 4 Position 5A 5210600 UK Power Strip 250V 6 Position 2A 5290400 European Power Strip 250V 4 Position 2A 5332600 Australian Power Strip 250V 6 Position 2A 5211501 Installation Tool 12 Electrical 9 Pin Sealed Pass Through Kit Part 5075619 Includes sealed 9 pin D subminiature with male pins on one side and female pins on the other connector and cord for data transfer to a typical printer outside the glove box from the balance Note Order the 9 pin installation kit separately which includes all the drill bits and hole saws for field installation Part 5241100 13 Mechanical or Electrical Straight Pass Through Kit Part 5240700 Provides three ID pass through ports with sizes of 230 395 230 530 and 450
11. 40 50 60 70 Airflow Volume Cubic Feet per Minute For thimble ducted glove boxes the proper remote blower selection can be determined from these exhaust requirements as the remote blower only needs to be sized for the static pressure loss of the ductwork system The thimble ducted exhaust blower should be sized for 25 additional airflow When exhausting a glove box to the outside 1t should be connected to a dedicated remote blower Labconco offers accessory remote blowers listed in Chapter 7 Contact Labconco for blower sizing assistance When a glove box 1s connected to a remote blower exhaust system an adjustable damper or valve should be installed in the duct to control the airflow volumes properly See Chapter 7 for Exhaust Dampers Electrical Requirements Electrical services should be nearby for connecting the glove box and accessory equipment The glove box is rated 115V 8A or 230V 4A or 100V 8A A convenience duplex outlet is located inside the glove box and 1s rated for 115V 5A or 230V 2A or 100V 5A A four outlet power strip is included with the 115V XPert Weigh Box for connection to the duplex outlet International outlet strips are listed in Chapter 7 Space Requirements The dimensions are shown in Appendix B Dimensions Provide adequate space to the right of the transfer chamber for loading and unloading Chapter 3 Getting Started Once the site for your XPert Weigh Box is properly prepared you are ready
12. 705 Order the installation kit with drill bits separately Part 5240800 Useful for sealing cords and plumbing 14 FilterMate Portable Exhausters and Filters for Secondary Filtration see models and filters below The FilterMate Portable Exhauster can successfully adsorb low level organic vapors formaldehyde ammonia and secondary particulates via another HEPA filter 3970001 115V FilterMate Carbon Filter sold separately Carbon Filter separately Carbon Filter separately 15 5 Thimble Exhaust Connector for Connection to the FilterMate Part 5241900 Connects to the top of the blower on the glove box for attaching the FilterMate Portable Exhauster The thimble is necessary for balancing the blower in the glove box with the FilterMate Portable Exhauster 16 6 Thimble Exhaust Connection to Building Exhaust Part 5230200 Provides a 6 exhaust thimble connection to the blower on the glove box for exhausting to the outside The thimble is necessary for balancing the blower on the roof with the glove box blower 17 6 Hard Duct Connection to House Exhaust Part 5230400 Provides a 6 closed hard duct connection for using a roof mounted blower and by passing the blower on the glove box 18 Remote Wall Mount Blower Kit Part 5229800 Provides a wall mount bracket 4 dia x 6 long hose hose clamps and 8 power cord for moving the blower from the glove box and remotely mounting it to a wall within 19 6 Exhaust Da
13. C e Maximum relative humidity 80 for temperatures up to 88 F 31 C decreasing linearly to 50 relative humidity at 104 F 40 C e Main supply voltage fluctuations not to exceed 10 of the nominal voltage e Transient over voltages according to Installation Categories II Over voltage Categories per IEC 1010 Temporary voltage spikes on the AC input line that may be as high as 1500V for 115V models and 2500V for 230V models are allowed e Used in an environment of Pollution degrees 2 1 e where normally only non conductive atmospheres are present Occasionally however a temporary conductivity caused by condensation must be expected in accordance with IEC 664 Appendix D lonizer Fan Performance Criteria Surface DC Voltmeter Static Electricity Test The tests were performed by monitoring the level of static electricity found on the work surface and interior surfaces within the glove box Static electricity levels were measured with an AlphaLab Surface DC Voltmeter http scientificmeter com surface_dc htm A DESCO Emit Mini Zero Volt Ionizer http www descoemit com ViewProduct aspx pid 506618 h 1323 was installed in the glove box The DESCO ionizer is a dual steady state DC auto balancing bench top ionizer The ionizer helps to neutralize static electricity on surrounding surfaces The time required by the ionizer to neutralize static electricity will be the shortest when objects are within 12 to 36 directly
14. R DOOR STEP 4 OPEN THE OUTER DOOR AND REMOVE THE COVERED MATERIALS Figure 5 1 Loading and Final Purging Technique HEPA Filter Applications Suitability and Guidelines Microbiological materials and hazardous solids containment protecting only the operator and the surrounding environment Weighing of powders Containment of hazardous particulates and powders using a HEPA filter Powder or particulate procedures traditionally performed on an open bench The HEPA filtered glove box provides personal and environmental protection from particulate matter hazardous powders and microbiological materials This glove box can be used for sample protection by reversing the blower airflow direction causing the main chamber to be under positive pressure Positive Pressure Conversion Kit is required see Chapter 7 Manipulations that generate gases or vapors 1 e toxic chemicals or radionuclides require the use of a HEPA and carbon filter in combination Otherwise the glove box should be ducted to the outside Each filtered glove box 1s provided with two HEPA filters Follow the HEPA Filter change procedure in Chapter 6 for disposal instructions For microbiological materials follow the Decontamination Procedure in Chapter 6 for disposal instructions Definition of Terms NIOSH National Institute for Occupational Safety and Health Mine Safety and Health Administration U S A TWA Recommended Exposure Limits expressed
15. Re Certification Fluorescent Light Replacement Motorized Impeller Replacement Speed Control Replacement Ionizer Fan Maintenance CHAPTER 7 ACCESSORIZING YOUR GLOVE BOX CHAPTER 8 TROUBLESHOOTING APPENDIX A REPLACEMENT PARTS APPENDIX B DIMENSIONS APPENDIX C SPECIFICATIONS 41 42 44 45 APPENDIX D IONIZER FAN PERFORMANCE CRITERIA 46 APPENDIX E PRESSURE amp FLOW RATE CONVERSIONS 48 APPENDIX F REFERENCES DECLARATION OF CONFORMITY 49 50 Chapter 1 Introduction Congratulations on your purchase of a Labconco XPert Weigh Box Your glove box provides personnel protection through superior containment rated at Class 1 conditions for hazardous environments per ISO 10648 2 The XPert Weigh Box has two added features that provide an added benefit during weighing procedures These two features include the stainless steel covered marble slab and the anti static ionizer fan These glove boxes will effectively contain toxic or noxious particulates when properly installed and operated In positive or negative pressure operation modes the glove box can create an ISO standard Class 3 ISO 14644 1 clean air environment This glove box uses two HEPA filters rated at least 99 99 efficient for 0 3 micron particles The XPert Weigh Boxes offer many unique features to enhance safety and performance To take full advantage of them please acquaint yourself with this manual and keep it handy for future reference All glove
16. ST OUTLET HEPA FILTER INLET HEPA FILTER 4 Pile Figure 3 3 Positive Pressure Glove Box Configuration Installation of Gloves to the Glove Ports With thumbs up and right left orientation secure the gloves in place on the glove ports by stretching the beaded cuff into the groove nearest the window Install the separate 8 diameter O ring over the gloves and into the outer groove of the glove port surface Stainless steel band clamps are provided for securing the separate O ring into the glove port groove Replacement gloves and parts are listed in Appendix A with a decription in Chapter 7 ge Oe A Z a a e CLAMP SECURES O RING INTO THE OUTER GROOVE OVER THE GLOVE 1 O RING INSTALLS IN OUTER GROOVE OVER GLOV GROOVE NEAREST WINDOW LOVE BEADED CUFF INSTALLS Y IN GROOVE NEAREST OUTER GROOVE NEO Figure 3 4 Glove Installation 12 Product Service 1 800 522 7658 Connecting the Electrical Supply to the Glove Box 115V 60 Hz 100V 50 Hz or 100V 60 Hz Models Connect the power cord supplied to the IEC electrical supply plug on the back of the glove box 115V or 100V models are rated at 8 amps total The maximum circuit load for the interior electrical duplex is 5 amps 230V 50 Hz or 230V 60 Hz Models The same procedure applies for the 230V model except it is shipped without a plug Install the appropriate plug per local requirements The 230V 50 Hz or 230V 60 Hz models are both rated at 4 amps total The inter
17. ached Work Surface Part 5235500 w casters Part 5235501 w adjustable feet These stands 60 x 31 with attached black laminate work surfaces have an adjustable height range from 33 to 40 and include a lower shelf for supporting accessory equipment 4 Blower Foot Switch Part 5241600 Provides a hands free means to shut off power to the built in blower 5 Bag Set for Bag in Bag out HEPA Filter Replacement Part 5241700 Set of two convenience bags and two safety straps for changing and disposing of one HEPA filter safely with a bag in bag out procedure 6 Anti Static Ionizer Fan Part 5234400 100 115V or Part 5234401 230V Included with the XPert Weigh Box Used for weighing operations and attaches to the ceiling of the glove box The ionizer circulates ionized air inside the glove box to reduce static charge Ionizer Replacement Points set of 8 emitter electrodes are ordered separately Part 5235100 See Appendix D for additional information 7 Balance Vibration Isolator Part 5234600 Included with the XPert Weigh Box Isolates building vibration and aids with balance weighing Includes a 20 x 13 x 2 1 4 marble slab with isolator pads and type 304 stainless steel top cover to protect the porous marble 8 Pressure Gauge for the Transfer Chamber Part 5238100 Kit includes the pressure gauge hose and fittings to add to the transfer chamber Range 0 to 29 inches of mercury vacuum 9 Add A Valve Kit Part 522570
18. afety Precautions l 10 11 12 It 1s the responsibility of the user to determine the suitability of this product for the intended applications Consult your Safety Officer Prior to using the glove box check to make sure that the exhaust blower 1s operating and that air 1s entering the glove box by observing the static pressure gauge Use good housekeeping in the enclosure at all times Clean up spills immediately Periodically clean glove box interior This product is not designed or intended to be explosion proof It is the responsibility of the user to determine and avoid lower explosive limits and flammability of any enclosed chemicals or gases The user is also responsible for taking proper precautions to prevent equipment damage or injury due to explosion or combustion Avoid the use of flammable gases or solvents in the glove box Care must be taken to ensure against the concentration of flammable or explosive gases or vapors Use of an open flame or exposure to high heat should be avoided in the glove box as heat can damage the liner Heat may harm the HEPA filter and damage the filter s adhesive Perchloric acid use in this enclosure is prohibited Radioisotope usage in the glove box should be cleared with your Safety Officer A qualified certification technician should test the integrity of the HEPA filters before the box is initially used The glove box should be recertified whenever it is relocated
19. amage that may have occurred in transit If damaged notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier THE UNITED STATES INTERSTATE COMMERCE COMMISSION RULES REQUIRE THAT CLAIMS BE FILED WITH THE DELIVERY CARRIER WITHIN FIFTEEN 15 DAYS OF DELIVERY DO NOT RETURN GOODS WITHOUT THE PRIOR AUTHORIZATION OF LABCONCO UNAUTHORIZED RETURNS WILL NOT BE ACCEPTED 41 IF ENCLOSURE WAS DAMAGED IN TRANSIT YOU MUST FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER LABCONCO CORPORATION AND ITS DEALERS ARE NOT RESPONSIBLE FOR SHIPPING DAMAGES Do not discard the packing material until you have checked all of the components and tested the glove box Installing the Glove Box on a Supporting Structure and Work Surface Exercise caution when lifting or moving the glove box When installing the glove box onto a work surface or benchtop ensure that the structure can safely support the combined weight of the glove box and any related equipment The work surface should be as wide as the glove box to properly support it The front of the glove box should be aligned with the front of the work surface for optimal comfort A height of 35 40 889mm 1016mm is appropriate for standing operation Adjustable Height Base Stands listed in Chapter 7 may be adjusted from 33 40 838mm 1016mm Connecting to the Exhaust System If Applicable WARNING The weight of any exhaust ductwork system must
20. aust HEPA filter can be checked from the blower inlet suction side test port Connect this tube to the downstream sampling port of the photometer Acceptance The downstream average penetration shall not exceed 0 005 percent as measured by the photometer Procedure When Using the ATI 2 D or 2 E Photometer NOTE Because the downstream side of the HEPA filters cannot be scanned a downstream average must be taken 1 Turn on the photometer and allow it to operate for a minimum 5 minutes Leave the range switch at 100 and the valve in the CLEAR setting 2 Use the screw on the face of the gauge to adjust the meter to 0 1f necessary 3 Ifthe INT REF switch is a square button depress it The switch will remain depressed and illuminated If the switch 1s a toggle type switch push and hold the switch to the ON position Keep this switch in the ON position through step 4 4 Turn the GAIN knob until a value of 15 is obtained Set for 100 CFM adjust for other airflows 5 Turn the range switch to 0 1 Turn the Stray Light knob until the meter reads 0 6 Turn the valve to the DOWNSTREAM setting Place the palm of your hand over the sampling port of the pistol There should be a strong vacuum at this port Return the valve to the CLEAR setting 7 Ensure that the glove box speed control is adjusted to the maximum setting and the blower is exhausting 100 CFM through the glove b
21. box operators must read and understand how HEPA filtered glove boxes operate please review Chapter 4 Performance Features and Safety Precautions before beginning to work with this glove box Even if you are an experienced user please review Chapter 5 Using Your XPert Weigh Box which describes the XPert features so that you can use the filtered glove box effectively and safely Chapter 2 Prerequisites Before you install the glove box you need to prepare your site for installation You must be certain that the area is level and of solid construction In addition a dedicated source of electrical power should be located near the installation site to power the glove box The glove box should be strategically placed in the lab to provide efficient workflow and prevent operator interference from normal traffic patterns Carefully read this chapter to learn the requirements for your installation site e The support vibration and preventive requirements e The location requirements e The exhaust and blower requirements e The electrical power requirements e The space requirements Refer to Appendix B XPert Weigh Box Dimensions for complete glove box dimensions Refer to Appendix C XPert Weigh Box Specifications for complete electrical and environmental conditions specifications and requirements Support Vibration and Preventive Requirements In the preparation of a glove box site please consider the following e A bench or stan
22. ch to complete the change process For your safety wear appropriate personal protective equipment during the change of HEPA filters If working with micro biologicals use the decontamination procedure referenced above Consult your Safety Officer before performing filter changes 1 Turn the glove box OFF Refer to Appendix A for replacement parts and Appendix B for dimensions 2 Loosen the six filter nuts that secure each HEPA filter 3 Place the accessory bag over the HEPA filter and seal to the filter housing 4 Place both arms in the rear gloves of the bag and carefully draw the HEPA filter out of the glove box Rest the HEPA filter on the floor table or cart Remove arms from bag gloves 5 Create two seals between the glove box housing and the old HEPA filter Seals can be heat sealed or sealed with tie wraps Cut off the bag between the two seals and leave the bag stub on the access port to the glove box 6 Place the new HEPA filter in another new bag Then place the new bag with the new HEPA filter over the old bag stub and onto the glove box filter housing 7 Carefully remove the old bag from the filter housing using the single glove near the access port Draw the old bag and stub into the single glove and turn the glove inside out Double seal this glove on the contaminated bag stub inside Remove the bag and dispose of properly 8 Carefully install the new HEPA filter and uniformly tighten the six filter nuts unt
23. d that is rigidly mounted to the floor or fixed to the wall but not both may be appropriate 35 to 40 889mm 1016mm 1s typical for standing height Labconco stands offered in Chapter 7 vary in height from 33 to 40 e The corners of a building typically have less vibration than the center which promotes analytical balance stability e The bench typically should not contain any vibration producing equipment such as shakers or pumps e A marble slab with dampening pads placed within the enclosure is an effective low cost means of controlling vibration and one is included with the XPert Weigh Box Location Requirements The XPert Weigh Boxes have been designed to rest on a typical 29 30 737mm 762mm deep work surface The height should be 35 40 for standing position Avoid placing the glove box in high traffic areas where walking might disrupt the operator or experimentation Exhaust and Blower Requirements The XPert Weigh Box as manufactured uses an integral motorized impeller to pull room air through the inlet and exhaust HEPA filters and discharge the clean air back into the laboratory This negative pressure operation mode pulls all internally contaminated air through the exhaust HEPA filter The HEPA filtered exhaust air is then forced out the top of the glove box An optional FilterMate Portable Exhauster with a carbon filter may be installed downstream of the exhaust to adsorb low levels of organic fumes formaldehy
24. de or ammonia gases The HEPA filtered exhaust air from the glove box can be exhausted to the outside with the installation of a thimble exhaust connection kit and remote blower listed in Chapter 7 As an option 1f necessary the built in motorized impeller can be wall mounted Labconco does not recommend a hard duct connection using the built in motorized impeller as the source of airflow out of the facility This creates a positive pressure in the exhaust duct Labconco does not recommend hard duct connections to remote blowers without a thimble due to the air volume balance requirements with such systems Hard duct connections should only be used when the glove box impeller is disconnected and only a single roof mounted blower is used Note that reliance on an external exhaust blower does not provide airflow control at the glove box If a positive internal pressure clean air ISO Class 3 condition ISO 14644 1 is desired the motorized impeller can be rotated to work in positive pressure mode see Figure 3 3 See Chapter 7 for the Positive Pressure Conversion Kit See the following Negative and Positive Pressure Particle Test data in the charts below Both charts show the dilution rates of the glove boxes XPert Weigh Box Particle Testing under Negative Pressure NOTE Ambient Air Baseline Particle Count at 1 Minute 294132 Particles measuring 0 3 Micron detected within 1 Minute 33296 Particles measuring 0 5 Micron detected within 1 Minu
25. der coated steel 5247300 Bin Organizer 5 4 plastic 5247301 Bin Organizer 7 4 plastic 30 31 DOP Test Kit for HEPA Filters Part 5242400 Includes the connections and hoses to carry out the HEPA Filter Leak Test documented in Chapter 6 of this manual Drying Train Tubing Kit Includes the FEP tubing and plumbing fittings for connecting typical moisture and other gas fume removal components see item 23 above Fluorinated ethylene propylene FEP tubing has very low moisture absorption 5242500 Drying Train Tubing Kit Moisture Solvents and Radioisotopes 32 HEPA and ULPA Filters HEPA filters come standard with the glove box ULPA filters may be substituted as an upgrade 5225105 Replacement Standard HEPA Filter 99 99 efficient on 0 3 micron particles 34 DI 36 5225106 Optional ULPA Filter 99 999 efficient on 0 3 micron particles 33 Gloves see Appendix A Replacement Parts for glove sizes and material types Neoprene gloves are the most resistant to abrasion and tearing Butyl gloves provide higher impermeability and improved dexterity Hypalon gloves are made with a mixture of neoprene and butyl rubber to provide an intermediate level of neoprene and butyl features Transfer Chamber High Vacuum Sleeve Part 5236200 An internal steel reinforced transfer chamber sleeve is required if a vacuum will be pulled in the transfer chamber Waste Chute Part 5245800 The glove box
26. e filtered glove box is connected to an electrical service in accordance with local and national electrical codes Failure to do so may create a fire or electrical hazard Do not remove or service any electrical components without first disconnecting the glove box from electrical service Used HEPA filters may be hazardous waste The user is responsible for recording chemicals used and disposal of used filters Ensure only trained operators use the glove box New users must review the User s Manual with emphasis on Routine Daily Work Procedures in Chapter 5 and become familiar with the safe operation of the glove box Only powders and particulates removed by HEPA filters are appropriate for use in this glove box Vapors and other gases should be exhausted to the outside or adsorbed with the use of an accessory FilterMate Portable Exhauster equipped with carbon filters See Chapter 7 Leave the impeller on for at least 5 10 minutes at 50 100 speed after work in the glove box has been completed to purge the interior of containments If a chemical powder or particulates are spilled on the work surface DO NOT switch off the blower until all traces have been removed Tag glove box with appropriate warning if filters have been removed or the glove box requires servicing If the blower fails during use processes should cease and the area should be vacated Inform your Safety Officer immediately Consult your Safety Officer before remov
27. e inlet concentration or the evaporation rate of the chemical must never exceed the TWA for the chemical Consult a Labconco Technical Specialist for estimated saturation life See Chapter 6 for an example of estimating saturation life based on Labconco s chemical guide Prohibited Acid Use The XPert Weigh Box motorized impeller and internal metal parts must not be exposed to mineral acid use No exceptions are permitted as the glove box life span will be limited with acid use NOTE If mineral acids are used with the XPert Weigh Box Labconco makes no claims about life span and warranties will become void Chapter 6 Maintaining Your Glove Box Review this chapter on maintenance for the following Routine maintenance Decontamination Determining when to replace the HEPA filters How to install new HEPA filters HEPA filter leak test Initial certification Re certification Fluorescent light replacement Motorized impeller replacement 10 Speed control replacement 11 Ionizer fan maintenance So eee ee Routine Maintenance Schedule Weekly e Wipe down the interior surfaces of the glove box with a disinfectant or cleaner depending upon the application e Using a damp cloth clean the exterior surfaces of the glove box particularly the front and top to remove any accumulated dust e Operate the exhaust system noting the airflow through the glove box to effectively remove a source of visible smoke Month
28. eriodic design changes without obligation to notify any person or entity of such change Warranty Labconco provides a warranty on all parts and factory workmanship The warranty includes areas of defective material and workmanship provided such defect results from normal and proper use of the equipment The warranty for all Labconco products will expire one year from date of installation or two years from date of shipment from Labconco whichever is sooner except the following e Purifier Delta Series Biological Safety Cabinets and PuriCare Lab Animal Research Stations carry a three year warranty from date of installation or four years from date of shipment from Labconco whichever is sooner SteamScrubber amp FlaskScrubber Glassware Washers carry a two year warranty from date of installation or three years from date of shipment from Labconco whichever is sooner Blood Drawing Chairs carry a ten year warranty Carts carry a lifetime warranty Glassware is not warranted from breakage when dropped or mishandled This limited warranty covers parts and labor but not transportation and insurance charges In the event of a warranty claim contact Labconco Corporation or the dealer who sold you the product If the cause is determined to be a manufacturing fault the dealer or Labconco Corporation will repair or replace all defective parts to restore the unit to operation Under no circumstances shall Labconco Corporation be liable
29. gauge reading has increased two fold The gauge can be changed to positive pressure for clean air glove box operations by reversing the hose on the gauge to the positive pressure connection Positive Pressure Conversion Kit is sold separately see Chapter 7 Glove Ports are 8 nominal size and molded into the main glove box shell The double grooves provide two points of restraint for safe changing of the gloves Neoprene Gloves are supplied with the glove box 015 thick 30 long one pair size 9 4 See Appendix A for replacements parts and Chapter 7 for a description Removable Framed Glass Window is 1 4 thick laminated safety glass and is reinforced with a powder coated steel frame The removable glass window frame provides access to the interior for loading large apparatus When removed the window opening is 26 4 wide x 12 5 high 670mm wide x 317mm high The framed glass window is sealed with a one piece molded neoprene gasket See Chapter 7 for optional Acrylic Viewing Window with Frame Fluorescent Lamp provides 30 40 foot candles of interior illumination on the work surface The lamp may be replaced from outside the glove box See Chapters 6 and 7 for replacement Control Panel consists of the main chamber static pressure gauge switches for the light blower duplex outlet and speed control The speed control can vary the impeller speed from 13 to 100 CFM Work Surface is black chemically resistant phenolic core and sealed
30. he main chamber by controlling the frequency of main chamber air volume changes See Figure 4 1 to refer to a detailed description of the main performance features 1 Molded One Piece Chemical Resistant Glove Box Shell made of medium density polyethylene which provides superior chemical resistance The molded liner is seamless and easy to clean with coved interior corners 2 Versatile Vibration Isolated Motorized Impeller Isolation supports eliminate the transfer of vibration to the work surface which is extremely important in weighing operations with precision analytical and micro balances The impeller wheel 1s also dynamically balanced capable of a minimum airflow of 13 CFM to a maximum of 100 CFM Measured displacement for vibration is less than 1 5 x 10 inches Additional unique features of the impeller include an optional Positive Pressure Kit a Recirculation Kit and a Blower Wall Mount Kit see Chapter 7 3 HEPA Filter System consists of the inlet and outlet HEPA filters rated 99 99 efficient on 0 3 micron particulates Inlet and outlet HEPA filters are identical and include a test port for challenging the HEPA filter integrity An optional filter bag may be ordered for bag in bag out filter change procedures see Chapter 7 10 11 12 13 14 Main Chamber Static Pressure Gauge provides an indication of HEPA filter condition inside the glove box Typically HEPA filters should be replaced when the static pressure
31. il the HEPA filter gasket is compressed 50 9 Turn the glove box ON and proceed to the HEPA Filter Leak Test in Chapter 6 OLD HEPA FILTER SEALED IN OLD BAG CREATE TWO SEALS AND CUT AFTER SEALING CREATE TWO SEALS AND CUT AFTER SEALING OLD BAG STUB SHOWN IN GLOVE OF NEW BAG Figure 6 1 HEPA Filter Changing Diagram HEPA Filter Leak Test Purpose This test is performed to determine the integrity of the HEPA filter s the filter housing and the filter mounting frames The leak test 1s to be done by a qualified technician with calibrated equipment Reference Leak Testing and Photometer scanning from the Institute of Environmental Services IES RP CC001 3 Equipment Required 1 An aerosol photometer ATI model 2D 2E 2G or equivalent Air Techniques Hamilton Associates inc 11403 Cron Ridge Dr Owings Mills MD 21117 2 One aerosol generator of the Laskin nozzle s type An aerosol of mineral oil or suitable liquid shall be created by flowing air through it The compressed air supplied to the generator should be adjusted to a pressure as indicated in the procedure Air Techniques Inc Model TDA 4A or equal 3 Mineral oil Labconco P N 1491400 4 Sampling Nozzle Rectangular 1 2 x 3 1 4 Air Techniques Inc Procedure When Using the ATI 2G Photometer NOTE Because the downstream side of the HEPA filters cannot be scanned a downstream average must be taken 1 Turn on the photometer and allow it to opera
32. ilter changes e Moving the glove box to a new location e Changing research programs The procedures for performing a gaseous decontamination are thoroughly outlined in the U S Department of Health Education and Welfare booklet entitled Formaldehyde Decontamination of Laminar Flow Biological Safety Cabinets available from NIH Division of Safety Bethesda MD 20892 call 301 496 2801 Determining When to Replace HEPA Filters The HEPA filters in the filtered glove boxes gradually accumulate airborne particulate matter and powders from the enclosure and room The rate of accumulation will depend upon the cleanliness of the room air the amount of time the enclosure is operating and the nature of work being done in the enclosure In typical installations and usage the HEPA filters will last two to five years before requiring replacement Replace HEPA filters if the speed control is adjusted to full speed or if the normal operating speed and or static pressure is double the original starting point Replace HEPA filters 1f it fails the HEPA Filter Leak Test in Chapter 6 How to Install New HEPA Filters The XPert Weigh Boxes include a true bag in bag out filter disposal system for safely removing the HEPA filter and protecting the worker from toxic powders and particulates see Figure 6 1 Filter Changing Diagram The accessory bags can be ordered from Labconco see Chapter 7 Two bags will be required for each HEPA filter for a total of 4 ea
33. ing any hoses to the static pressure gauge or exhaust hoses as they may be contaminated Chapter 5 Using Your Glove Box Now that the installation of your glove box is completed you are ready to use your XPert Weigh Box Read this chapter to learn about Routine Daily Work Procedures Appropriate HEPA Filter Applications Suitability and Guidelines Carbon Filter Exhaust Applications Definition of Terms Appropriate Chemicals for Carbon Filters Hazardous Misapplications for Carbon Filters Chemical Carcinogen use with Carbon Filters Acid Use Routine Daily Work Procedures Planning Thoroughly understand procedures and equipment required before beginning work Start up Consult your Safety Officer for personal protective equipment recommendations Turn on exhaust system and light Allow the enclosure to operate for 2 5 minutes at 50 100 blower speed Loading Materials and Equipment see Figure 5 1 Load only the materials required for the procedure Do not overload the glove box Be aware that gloves will inflate when the glove box is operated under negative pressure Ensure items in the glove box will not obstruct the movement of the gloves After loading wait 2 5 minutes at 50 100 blower speed to purge airborne contaminants from the work area before beginning procedures Work Techniques Segregate all clean and contaminated materials in the work area Final Purging see Figure 5 1 All open trays
34. ionizer and then into the appropriate AC power source The ionizer has no On Off switch so it should be running as soon as it is plugged in The ionizer is designed to run off of 24VDC 10 300mA The balance adjustment for the ionizer can be accomplished by inserting a small screwdriver or trimmer adjustment tool into the balance adjustment hole located on the side of the unit To increase the output in a positive direction turn the potentiometer in a clockwise direction Conversely to increase the output in a negative direction turn the potentiometer in a counter clockwise direction It 1s recommended that you use a surface DC voltmeter when adjusting the balance of the ionizer Under normal conditions the ionizer will attract dirt and dust especially on the emitter electrodes To maintain optimum neutralization efficiency and operation cleaning with isopropyl alcohol should be performed on a regular basis If the performance of the ionizer degrades because of dirty or corroded points the LED on the front of the unit will turn YELLOW the audible alarm will sound continuously Under this condition the unit is not able to maintain balanced ionization The input power cord must be disconnected before the unit 1s opened for maintenance The emitter electrodes should be cleaned using the alcohol cleaners included or a swab wet with isopropyl alcohol Unscrew the 4 screws on the back of the unit and then remove the screen After cleaning the emit
35. ior electrical duplex is rated at 2 amps Setting the Exhaust Volume with the Speed Control Adjustment For XPert Weigh Boxes adjustment of the speed control provides control of exhaust air volume and is located on the front panel The exhaust air volume should be adjusted at the low setting for weighing operations Consult your Safety Officer for airflow recommendations for your application Working at the lowest exhaust volume appropriate for the application will give the quietest operation To increase the rate of air volume changes after working in the glove box and to ensure proper cleaning the speed control should be set at a higher 50 to 100 dial setting for 5 to 10 minutes as recommended by the operator s predetermined protocol and the chart below The exhaust volume is increased by turning the speed control counterclockwise Minimum Dial Setting Approximate Estimated Recommended for Cleaning Air Volume Total Exhaust Times to Internal Air Changes per Volume Clean Internal Volume Minute CFM Air Volume minutes Validating the Glove Box The XPert Weigh Box has been leak tested at the factory for HEPA filter and main chamber pressure integrity The HEPA filters should be leak checked upon start up and annually thereafter per the procedure in Chapter 6 Upon start up the main chamber pressure should be greater than 0 7 w g at full blower speed to validate proper blower operation if not contact Labconco for service
36. lace HEPA filters if pressure doubles or airflow is reduced to half the original volume e Perform HEPA Filter Leak Test e Perform a Smoke Test to determine proper dilution rate inside the glove box Smoke removal should be completely purged in less than two minutes at full blower speed Fluorescent Light Replacement 1 Disconnect the power Refer to Appendix A for replacement parts 2 Locate the light reflector located on the top of the glove box behind the front panel 3 Remove the light reflector support by removing two hex nuts located on both sides of the light reflector 4 Remove the old fluorescent lamp 5 Reinstall the new fluorescent lamp and light reflector by reversing the steps above Motorized Impeller Replacement The motorized impeller must be replaced as a complete unit See Appendix A for replacement parts diagram l Unplug the motorized impeller from the electrical outlet Refer to Appendix A for replacement parts Remove the hose connecting the exhaust HEPA filter to the motorized impeller Remove the motorized impeller and support braces from the light and the rear sheet metal facade Reassemble the new motorized impeller by reversing the steps above WARNING High speed blower Never operate impeller with housing off Speed Control Replacement l Disconnect the power and remove the cover behind the front control panel where the speed control 1s located Remove the two fasteners ho
37. lding the speed control Refer to Appendix A for replacement parts Disconnect all wires leading to the speed control Connect wires on new speed control in the same position as the old speed control Reassemble to the system in the same position and with the same fasteners that were removed earlier lonizer Fan Maintenance l To maintain optimum neutralization efficiency and operation the ionizer fan should be cleaned on a regular basis with the alcohol cleaners provided or a swab wet with isopropyl alcohol The input power must be turned OFF before the unit 1s opened up for maintenance The emitter electrodes ionizer points should be cleaned with the alcohol Unscrew the four screws on the back of the unit and remove the screen to clean the emitter electrodes After cleaning reinstall by reversing the steps above See Appendix D for additional information Chapter 7 Accessorizing Your Glove Box There are many ways to accessorize and modify the glove box for your individual requirements These include many different accessories listed in this chapter 1 Black Epoxy Flat Work Surface Part 4882807 A solid epoxy resin 60 x 30 x 1 25 work surface 1s available to support the glove box 2 30 Standard Base Cabinet Part 9900200 Two optional base cabinets can be ordered to support the 60 x 30 work surface and glove box Contact Labconco for additional sizes 3 Adjustable Height Base Stands with Att
38. limits 0 05 PPM average or less e Sound levels Noise Pressure varied from 44 dB A at 13 CFM to 71 dB A at 100 CFM If vented to the outside the sound level will be greatly reduced e Airborne particulate cleanliness measured under positive and negative pressures within the main chamber of the glove box exceeded ISO Class 5 conditions equivalent to Class 100 and achieved ISO Class 3 conditions equivalent to Class 1 at both minimum and maximum operational airflow volumes Glove box fluorescent lamp light levels illumination measured across the work surface averaged 29 9 foot candles with room lights turned off and 33 7 foot candles with the test lab s overhead fluorescent lights turned on Static pressure measured inside the glove box varied from negative 0 14 inch of water column at minimum 13 CFM to negative 0 91 inch of water at maximum 100 CFM The total system static pressure measured between the exhaust filter and the blower varied from negative 0 34 inch of water column at 13 CFM to negative 2 4 inches of water at 100 CFM The vibration level displacement measured on the work surface 1s negligible at minimum through maximum airflow volume settings The measured level of displacement ranged from 0 59 x 10 inches to 1 45 x 10 inches The static electricity measured on the work surface and interior surfaces of the glove box achieved a negligible level below 100V on any surface with the glove bo
39. ly or more often as required e Chart the operating static pressure at a typical speed control setting Change HEPA filters when the static pressure increases two fold or speed setting increases two fold Guardian Jr Airflow Monitors are available for constant monitoring see Chapter 7 Accessory e While the glove box is filled with the contaminant gas test filter FilterMate condition of accessory FilterMate carbon filters using the appropriate Carbon gas detector tube at intervals of 20 of the total estimated time The exception to the 20 recommendation 1s formaldehyde or any carcinogen or suspected carcinogen These hazardous chemicals must be checked at least every 10 of the total estimated time Gas detector tubes for the specific chemicals that are being used in the glove box can be obtained from your laboratory supply dealer refer to FilterMate manual Accessory FilterMate Replace accessory FilterMate carbon filters when chemical breakthrough is indicated by odor time detector tube or for some chemicals analytical instrumentation Carbon Annually e Replace the fluorescent lamps e Have the glove box re certified by a qualified certification technician See Certification and Recertification in Chapter 6 Decontamination When used in conjunction with microbiologicals the glove box should be decontaminated with formaldehyde gas before e Performing maintenance work in contaminated areas e HEPA f
40. mpers Exhaust dampers allow airflow adjustments to be made maintaining proper airflow to roof mounted remote blowers or building exhaust 3924000 6 Epoxy Coated Steel In Line Adjustable Damper 4724200 6 PVC In Line Adjustable Damper 20 Remote Blowers Has a 1 4 hp direct drive motor and corrosion resistant epoxy coated steel housing and wheel with blower inlet of 6 00 ID Outlet dimensions are 4 25 x 7 38 OD CFM a Static Pressure Inches of H O SP 00 0 125 0 25 0 50 075 0 87 420 300 167 Part Description 4863500 Remote Blower 115 V 60 Hz 4 4 amps 4863501 Remote Blower 115 230 V 50 Hz 5 6 2 8 amps 4 HOLES All dimensions in inches 21 Positive Pressure Conversion Kit Part 5236900 Converts integral glove box blower from negative pressure operation to positive pressure 22 Recirculation Kit Part 5236500 Allows the blower exhaust to be connected to the HEPA inlet filter for recirculation of the exhaust air Useful with drying train components on the main chamber 23 Filtration Components for Moisture and Fume Removal The following components are useful in removing organics acids ammonia moisture and other gases as indicated They are connected to the glove box using the 5225701 Add A Valve Kit 5248200 Vacuum Pump for Circulation 115V 60 Hz w switch 5248201 Vacuum Pump for Circulation 230V 50 Hz 7815200 Moisture amp Solvent Trap Insert molec
41. or serviced and at least annually thereafter The use of safety goggles protective clothing and any other personal protective equipment recommended by your safety officer should be employed The HEPA filter provides personnel and environmental protection from particulate matter Should the blower be reversed to positive pressure inside this glove box may be used for operations requiring product protection from environmental particulate contamination HEPA filters are only effective for entrapment of particulate matter Manipulations that generate gases or vapors from toxic chemicals or radionuclides are not captured by the HEPA filters 13 14 15 16 17 18 19 20 21 22 23 The surface of the HEPA filter is fragile and should not be touched Care must be taken to avoid puncturing the HEPA filter during installation or normal operation If you suspect that a HEPA filter has been damaged DO NOT use the glove box contact a local certification agency or Safety Officer The HEPA filters in the glove box will gradually accumulate airborne particulate matter from the room and from work performed in the glove box The rate of accumulation will depend upon the cleanliness of the room air the amount of time the glove box is operating and the nature of work being done in the glove box With normal usage the HEPA filters will last two to five years before requiring replacement see Chapter 6 Ensure that th
42. or HEPA filter loading IDLH Immediately Dangerous to Life and Health An atmosphere that poses an immediate hazard to life or produces immediate irreversible health effects IDLH concentrations should not be approached in the glove box Appropriate Chemicals for Carbon Filters Below is a general set of rules to determine appropriateness of chemical usage Selected organic chemicals considered to be occupational carcinogens by NIOSH can be used in the filtered glove box with carbon filters under rigid restrictions See separate discussion on carcinogens for special instructions Organics must have time weighted exposure limits TWA of 1 PPM or greater Chemicals must have a detectable odor at concentrations below the TWA for the chemical Chemicals must be designated by NIOSH guidelines as acceptable for use with chemical cartridge type respirators the exception 1s formaldehyde and ammonia amines which use impregnated carbon Chemicals not listed by NIOSH in the Pocket Guide must be approved by Labconco Product Specialist or Engineering Inlet concentration must never exceed the IDLH Immediately Dangerous to Life and Health concentrations Chemicals having a recommendation by NIOSH of at least Escape GMFOV Gas Mask Full Face Respirator When evaporating a mixture of chemicals the chemical having the lowest TWA will be used to determine if the mixture meets the guidelines Call a Labconco Product Specialist a
43. ox 8 Position the aerosol generator discharge near the opening of each HEPA filter Check both filters 9 Start the aerosol generator Ensure that the generator is operating with nozzle on at 10 PSIG 10 Allow the generator to operate for a minimum of 15 seconds Place a 1 4 OD sampling tube in the exhaust downstream of the HEPA filters The inlet HEPA filter can be checked from the test port on the exhaust HEPA filter and the exhaust HEPA filter can be checked from the blower inlet suction side test port Connect this tube to the downstream sampling port of the photometer Acceptance The downstream average penetration shall not exceed 0 005 percent as measured by the photometer Initial Certification The XPert Weigh Box has been leak tested at the factory with helium gas at positive 1 w g pressure leaks cannot exceed 1 x 10 cc sec The HEPA Filter Leak Test has been performed at the factory on both HEPA filters with no particulate leaks greater than 005 percent The filtered glove box should be certified before use for the three tests listed under Re Certification below It is also recommended to perform the HEPA Filter Leak Test again should there be any damage caused during transport Re Certification Under normal operating conditions the glove box should be re certified at least annually if serviced The certifier should perform the following tests e Record airflow volume and glove box static pressure Rep
44. rtment or Labconco s Product Service Department at 1 800 821 5525 or 1 816 333 8811 between the hours of 7 00 a m and 6 00 p m Central Standard Time Part 5233703 Rev ECO D470 TABLE OF CONTENTS CHAPTER 1 INTRODUCTION CHAPTER 2 PREREQUISITES Support Vibration amp Preventive Requirements Location Requirements Exhaust and Blower Requirements ISO Class 3 Definition Electrical Requirements Space Requirements CHAPTER 3 GETTING STARTED Unpacking the Glove Box Installing the Glove Box on a Supporting Structure amp Work Surface Connecting to the Exhaust System If Applicable Installation of Gloves to the Glove Ports Connecting the Electrical Supply to the Glove Box Setting Exhaust Volume with Speed Control Adjustment Validating the Glove Box Summary of Performance Testing Results CHAPTER 4 PERFORMANCE FEATURES AND SAFETY PRECAUTIONS Performance Features Safety Precautions CHAPTER 5 USING YOUR GLOVE BOX Routine Daily Work Procedures HEPA Filter Applications Suitability amp Guidelines Definition of Terms Appropriate Chemicals for Carbon Filters Hazardous Misapplications for Carbon Filters with Volatile Chemicals Chemical Carcinogen Use with Carbon Filters Prohibited Acid Use SN MN UY UY UY IN CHAPTER 6 MAINTAINING YOUR GLOVE BOX Routine Maintenance Schedule Decontamination Determining When to Replace HEPA Filters How to Install New HEPA Filters HEPA Filter Leak Test Initial Certification
45. sease Control and Prevention National Institute for Occupational Safety and Health DECLARATION OF CONFORMITY Application Council Directive s 73 23 EEC 89 336 EEC 2002 95 EC ROHS 2002 96 EC WEEE Standard s to which conformity is declared EN61010 1 EN61326 1 Manufacturer s Name Labconco Corporation Manufacturer s Address 8811 Prospect Avenue Kansas City MO 64132 USA Importer s Name See Shipping Customs Documents Importer s Address See Shipping Customs Documents for your equipment Type of Equipment Laboratory Equipment Precise Glove Boxes 5220000 Series Precise Basic Glove Box 5220100 Series Precise Controlled Atmosphere Glove Box 5220200 Series Precise HEPA Filtered Glove Box 5220300 Series XPert Weigh Box Serial No Various See Individual Declaration Year of Manufacture 2006 and subsequent I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s See individual Declaration of Conformity which will be signed by the importer for your country Place Signature Date Full Name Position
46. t 1 800 821 5525 for assistance in chemical appropriateness Hazardous Misapplications for Carbon Filters with Volatile Chemicals There is one scenario where the accessory FilterMate carbon filter misapplication would be a part of a hazardous condition If the user continues to operate the glove box with FilterMate Portable Exhauster with any of the following conditions a potentially hazardous condition will exist 1 The inlet concentration of vapors is greater than the TWA The carbon filter becomes saturated 3 The ventilation of the room is insufficient to dilute the exhaust of the enclosure to below the TWA for the chemical When the inlet concentration is greater than the TWA extra measures must be taken to monitor the filter and number of room air exchanges Chemical Carcinogen Use with Carbon Filters Selected carcinogens may be used safely with accessory FilterMate carbon filters under the following restrictions outside is always the preferred method when working with carcinogens The carbon filters should only be used as a last resort when venting to the outside is not an option r The use of a ventilated glove box with ducting to the The potential carcinogens are listed in the NIOSH Pocket Guide to Chemical Hazards as Ca Each potential carcinogen must have a TWA of 1 or greater have minimum respirator recommendation of Escape GMFOV and an odor threshold significantly lower than the TWA for the chemical Th
47. te Airflow Testing Performed at 50 Dial Setting 61 CFM with Glove Box Under Negative Pressure e gt 0 3 Micron gt 0 5 Micron A S San a San 2 2 Q O Som e e Y 9 t a ra O pum 0 lo za 8 9 10 11 Time Minutes XPert Weigh Box Particle Testing under Positive Pressure NOTE Ambient Air Baseline Particle Count at 1 Minute 198671 Particles measuring 0 3 Micron detected within 1 Minute 25473 Particles measuring 0 5 Micron detected within 1 Minute A o o al o Airflow Testing Performed at 50 Dial Setting 61 CFM with Glove Box Under Positive Pressure e gt 0 3 Micron gt 0 5 Micron Number of Particles Per Cubic Meter Per Minute 3 Time Minutes ISO Class 3 Definition Airborne particulate cleanliness inside any clean air glove box or enclosure 1s designated by ISO Class 3 which is equivalent to 35 particles 0 5 um or larger per cubic meter of air per minute as defined by ISO Standard 14644 1 ISO Class 3 cleanliness 1s illustrated in the table below and is equivalent to Class 1 air conditions as defined by Federal Standard 209E or 1 particle 0 5 um or larger per cubic foot of air per minute Table 1 1 Selected airborne particulate cleanliness classes for cleanrooms and clean zones ISO Maximum concentration limits particles m of air minute for particles equal to
48. te for a minimum of 5 minutes Leave the valve in the CLEAR setting 2 Press the ENTER keypad Press the REF keypad 3 The display will show P1 or P2 for approximately 1 second and then display a numerical value 4 Using the A or W keypads increase or decrease the numerical value respectively until it equals 68 Set for 100 CFM adjust for other airflows 5 Press the ENTER Keypad The photometer will scan for 15 seconds and then the 0 keypad will flash Press the Enter keypad The unit will scan for 5 seconds the display will read 0000 and the unit will sound a confirming tone 6 Set the valve to DOWNSTREAM Place the palm of your hand over the sampling port of the pistol There should be a strong vacuum at this port If the vacuum is weak contact Air Techniques Hamilton Associates 7 Ensure that the glove box speed control is adjusted to the maximum setting and the blower is exhausting 100 CFM through the glove box 8 Position the aerosol generator discharge near the internal opening of each HEPA filter Check both filters 9 Start the aerosol generator Ensure that the generator 1s operating with 1 nozzle on at 10 PSIG 10 Allow the generator to operate for a minimum of 15 seconds Place a 1 4 OD sampling tube in the exhaust downstream of the HEPA filters The inlet HEPA filter can be checked from the test port on the exhaust HEPA filter and the exh
49. ter electrodes reinstall the screen and 4 screws The emitter electrodes should not require replacement during the life of the unit with normal handling Verify the balance of the ionizer with a surface DC voltmeter after cleaning Center Work Surface Appendix E Conversions Pressure Conversions el rfl ae dela From mmie Co 5 oaae 00052 00054 00m 158 0015 ing 2540 1 1500 185 0542 4912 054553307059 Sr TON a Conversions lit sec gal min ft sec ft min bbl hr bbl day From lit see CA 85 00035827 03532 2 WET 22 22 66 543 8 8 aati oe A Aa ao ft sec l MW OA NNA f s sie ooms 06997 oos ow 1 A MEL 000184 00317 6500 00090 004187 1 Appendix F References Many excellent reference texts and booklets are currently available The following is a brief listing Pharmaceutical Isolators A Guide to their application design and control Pharmaceutical Press 2004 Editors Midcalf Phillips Neiger and Coles Isolation Technology A Practical Guide 2004 CRC Press ge Edition www crcpress com International Standard ISO 10648 2 Containment Enclosures e Classification according to leak tightness and associated checking methods International Standard ISO 14644 7 Clean Rooms and associated controlled environments e Separate devise such as glove boxes and isolators NIOSH Pocket Guide to Chemical Hazards U S Dept of Health and Human Resources Centers for Di
50. tion 15A see Chapter 7 for other international versions e Appendix A Replacement Parts 13 NOT SHOWN 12 3 NOT SHOWN Note Items 14 15 amp 16 are not shown Figure A 1 Replacement Parts for XPert Weigh Box Product Service 1 800 522 7658 43 Appendix B Dimensions See the following dimensions and exhaust options for the XPert Weigh Boxes All dimensions are in inches REET j BLOWER POWER 6 1 ID EXHAUST DUPLEX EXHAUST AL HEPA 82 Iig K LAX A DK m 4 L e m a AE E Ah NOTE TRANSFER CHAMBER HAS A 12 0 K MINIMUM INTERIOR WIDTH ce no be CONTROLS INLET P HEPA 35 9 52 6 LIGHT MAIN CHAMBER BLOWER SPEED CONTROL 100 CFM MAX PRESSURE INDICATOR NTERI AUX OUTLET SWITCH de dai LIGHT SWITCH BLOWER SWITCH APPROX 13 CU FT MAIN CHAMBER 41 3 HARD DUCT 43 8 i 11 0 1D TRANSFER THIMBLE i VA CHAMBER 17 0 8 0 NOMINAL ERP GLOVE PORTS iS Figure B 1 44 Product Service 1 800 522 7658 Appendix C Specifications This Appendix contains technical information about all the glove boxes including electrical specifications and environmental operating conditions e 8 Amps 115V 50 60 Hz or 4 Amps 230V 50 60 Hz or 8 Amps 100V 50 60 Hz XPert Weigh Box Environmental Conditions e Indoor use only e Maximum altitude 6562 feet 2000 meters e Ambient temperature range 41 to 104 F 5 to 40
51. to the glove box floor The black surface aids proper cleaning and provides high visibility of powders Phenolic core has superior chemical resistance and stain resistance Interior Switched Duplex is located on the glove box ceiling The innermost outlet of the duplex labeled AUX is switch operated on the front control panel An additional power strip is included with 115V models See Chapter 7 for accessory power strips for international electrical configurations The interior duplex is rated at 115V 5A or 230V 2A or 100V 5A Transfer Chamber Doors have a molded one piece neoprene gasket with a quick operating latch for user comfort The counterweight doors swing up to save space IMPORTANT Transfer chamber must have an optional High Vacuum Sleeve if the chamber is converted for vacuum operation See Chapter 7 Anti Static Ionizer Fan is located on the ceiling of the glove box and switch operated on the control panel It circulates 1onized air inside the glove box to reduce static charge and is useful in weighing applications See Appendix D for Ionizer Fan Performance Criteria Balance Vibration Isolator includes a marble slab with isolator pads and stainless steel cover to protect the porous marble Provides stable platform for sensitive weighing applications Chapter 4 Performance Features and Safety Precautions _ 6 NOT SHOWN Note Items 13 amp 14 are not shown Figure 4 1 18 Product Service 1 800 522 7658 S
52. to unpack inspect install and validate your system Read this chapter to learn how to e Unpack and move the glove box e Set up the glove box with the proper supporting structure and work surface e Connect to an exhaust system if applicable e Install the gloves e Connect the electrical supply e Set the exhaust volume with the speed control adjustment e Arrange validation for the glove box shipping container allows for lifting with a mechanical lift truck or floor jack If you must lift the enclosure manually follow safe lifting guidelines Do not lift by the upper sheet metal facade and front panel as damage can occur to the plastic glove box liner See Figure 3 1 7 Each XPert Glove Box weighs 275 lbs 124 kg The NEVER LIFT HERE AS DAMAGE CAN OCCUR NEVER LIFT HERE AS DAMAGE CAN OCCUR NEVER LIFT HERE AS DAMAGE CAN OCCUR LIFT HERE AT THE BOTTOM ONLY LIFT HERE AT THE BOTTOM ONLY LIFT HERE AT THE BOTTOM ONLY Figure 3 1 Lifting Instructions Unpacking the Glove Box We recommend that you do not remove the glove box from its shipping container until it is ready to be placed into its final location Note The vibration isolating marble slab is packaged in a separate cardboard carton attached to the main cardboard carton Move the unit by placing a flat low dolly under the shipping skid or by using a floor jack Carefully remove the carton shrink wrap on the glove box and inspect it for d
53. ular sieve 5244100 Flowmeter Visual 5240100 Gas amp Vacuum 0 2 Micron Filter 24 Acrylic Viewing Window with Frame Part 5236100 Acrylic Window 1 2 thick for those users that prefer acrylic over the standard laminated safety glass includes metal frame 25 Guardian Jr Airflow Monitor Part 5242000 Audio and visual airflow monitor 1s especially useful for installations that exhaust to the outside as 1t provides feedback on the roof mounted blower status Monitor mounts to the top of the control panel 26 Interior Storage Shelf Kits Provide convenient storage in the rear or the left side of the glove box to maximize space Additional hanging shelf kits sold separately can hang from the rear interior shelf kit Each shelf kit adds 240 square inches of usable space Will support a large 15 2 x 4 8 x 3 7 tissue box 5235000 Rear Storage Shelf Kit 5235001 Side Storage Shelf Kit 27 IV Bar Kit Part 5242100 Provides a bar for supporting IV bags Must be used with one of the interior storage shelf kits 5235000 or 5235001 28 External Glove Port Caps Part 5242200 Cap and clamp attaches to the glove ports to eliminate glove inflation during dilution or venting operations Sold as a set of two 29 Hanging Shelf Kits All shelves require the 5235000 rear interior storage shelf kit to function 3925000 Consists of three utility shelves coated steel 3927700 Bottle Holder coated steel 3927800 Tissue Hol
54. weigh vessels or containers must be sealed before being removed from the glove box Objects in contact with contaminated material must be surface decontaminated before removal from the glove box The interior surfaces should be cleaned per user s protocol Upon completion of work the glove box should be allowed to operate for 5 minutes undisturbed at 100 speed or 10 minutes at 50 speed to purge and dilute airborne contaminants from the work area After purging the main chamber the transfer chamber should also be purged for 5 minutes at 100 speed or 10 minutes at 50 speed with the inner door open before removing sealed material Shutdown Turn off the exhaust system and light Keep outer door closed when not in use INNER DOOR SHOWN OPEN FOR LOADING BLOWER MUST BE ON STEP BE SURE THE INNER DOOR IS CLOSED BEFORE OPENING THE OUTER DOOR STEP 2 LOAD MATERIALS AND CLOSE THE OUTER DOOR STEP 3 OPEN THE INNER DOOR AND MOVE MATERIALS STEP 4 NOW CLOSE THE INNER DOOR STEP 5 PURGE THE MAIN GLOVE BOX CHAMBER FOR 2 5 MINUTES BEFORE PROCEEDING WITH WORK Safety Note Must seal and wipe down all chemical containers FOR FINAL PURGING IBLOWER MUST BE ON STEP L PURGE THE MAIN GLOVE BOX CHAMBER FOR 5 MINUTES AT FULL SPEED OR 10 MINUTES AT HALF SPEED STEP 2 OPEN THE INNER DOOR MOVE COVERED MATERIALS TO THE TRANSFER CHAMBER AND PURGE FOR 5 MINUTES AT FULL SPEED OR 10 MINUTES AT HALF SPEED STEP 3 NOW CLOSE THE INNE
55. x blower operating at a min 13 CFM to max 100 CFM at 22 relative humidity Test conditions included the ionizer fan speed set to high output and the potentiometer within the ionizer was adjusted slightly between positive and negative output in order to maintain balanced ionization The time required for sufficient electrostatic decay will vary depending upon user application moisture levels within the supply air and the level of cleanliness within the glove box interior Anti Static Ionizer Fan is sold separately see Chapter 7 The helium leaks ranged from 2 x 10 to 1 x 10 ml sec with no individual leak greater than the specified 1 x 10 ml sec Balance stability testing results were excellent with no detectable instability of an analytical balance to five decimal places and a top loader balance to three decimal places Test results were confirmed for various sample weights with the glove box running at various blower airflow settings Chapter 4 Performance Features and Safety Precautions Performance Features The XPert Weigh Box is designed to meet the needs of the laboratory scientist and provide superior particulate containment The HEPA filtered XPert Weigh Boxes have been tested to effectively contain toxic and noxious materials when properly installed and operated Labconco engineered the HEPA filtered glove boxes to minimize the effects of turbulence The concentrations of particulate materials are predominantly removed from t

User's Manual - Fisher UK Extranet

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