Model 683 Small Animal Ventilator User's Manual
Contents
1. Accessories 55 5282 55 5283 73 3076 Publication 5375 001 REV F Adjustable from 30 to 150 strokes min while the ventilator is running 1 1 fixed 25 x 32 5 x 20 cm 10x 13 x 8 in 9 5 kg 21lbs 100 120 VAC 200 230 VAC 0 7A 0 35A max 50 60 Hz Continuously variable from 30 to 150 strokes min while the ventilator is running with LIGHT EMITTING DIODE LED DISPLAY Adjustable from 0 2 to 5 0 cc stroke or 1 5 to 30 0 cc stroke while the ventilator is running 0 C to 40 C 32 F to 104 20 to 80 RH non condensing ll 1 5121 074 128 8 25mm 188 4 78mm 55 0000 Overhaul Kit for 5cc Small Animal Ventilator contains O rings valve springs lubricants etc to overhaul Ventilator Overhaul Kit for 30cc Small Animal Ventilator contains O rings valve springs lubricants etc to overhaul Ventilator Connection Kit for Anesthesia Pressurized Gas Connection 2 Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Description amp Features The current MODEL 683 NEW STYLE WITH DIGITAL READOUT is an improved volume controlled small animal rodent respirator that is functionally identical to previous MODELS 680 681 and 683 OLD STYLE WITHOUT DIGITAL READOUT with the following major changes New stronger motor and motor speed control with LED light emitting diode read out of rate stroke rate or breaths min A new design of the vertical slide valve that has no O R2. and will comply with any changes as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required 2 Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Table of Contents SUBJECT PAGE NO General Information Warranty amp Repairs 2 General Safety Summary 3 Sp cifications iioi nae 4 Description amp Features 5 Voltage Selection eene 6 Fuse Replacement 6 Pump Operation Connecting to Animal 7 Selecting Rate amp Volume 8 Setting Volume amp Rate 9 Using Gas 9 Creating PEEP tee bb se 9 Cleaning amp Maintenance 10 Changing Piston amp Cylinder 11 12 Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual General Information Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will s
3. be installed depending on the chosen orientation The depth of the end of the tubing in cm below the water surface will give the PEEP pressure in cmH2O Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Maintenance Cleaning To clean the exterior surfaces use a lint free cloth to remove loose dust Use care to avoid scratching the clear display window For more efficient cleaning use a soft cloth dampened with water or an aqueous solution of 7596 isopropyl alcohol Maintenance No special maintenance is required other than periodic lubrication of the piston O rings and the slide valve with the special non toxic grease provided To get to the slide valve for lubrication l Remove the four screws holding the valve to the 1 4 aluminum black back plate 2 Remove the screws in front that hold the cylinder and valve together 3 Liftentire valve assembly up and out of Ventilator 4 Pull stainless steel valve rod free of valve block 5 Lubricate sparingly Model 683 Rodent Ventilator Replacement Parts e CYLINDER VALVE BLOCK 5 cc 0683 033 ASSEMBLY 30 cc 0683 034 0683 007 5 Ge DBD O48 5 5013 019 PISTON 2 O RING 1 30 0681 011 30 5013 027 BE H i Ex m 1 _ i 5 O RING 2 SCREWS 5013 017 44 40 x 1 1 8 HELICAL PISTON SHAFT 5101 16
4. for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample or drawing We offer a complete reconditioning service CAUTION This ventilator is not registered with the FDA and is not for clinical use on human or veteri nary patients It is intended for research use only CAUTION FOR RESEARCH USE ONLY NOT FOR CLINICAL USE ON PATIENTS Publication 5375 001 REV F 5 Harvard Apparatus Model 683 Small Animal Ventilator User s Manual General Safety Summary Please read the following safety precautions to ensure proper use of your ventilator avoid potential hazards and product damage use this product only as instructed this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired To Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the ground
5. 1995 EN 61000 4 6 1996 EN 61000 4 11 1994 EN 61000 3 2 2000 w A 14 EN 61000 3 3 1995 w 1997 Manufacturer s Name Harvard Apparatus Manufacturer s Address 64 October Hill Road Holliston Massachusetts 01746 U S A Type of Equipment Small Animal Ventilator Model No 683 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s EMC compliance was evaluated by Intertek ETL Semko Reference test report file number Box 3091685 001 Place United States of America Date February 2006 C Signature Beth Bauman HARVARD na VP Engineering Operations APPARATUS Position
6. 5 COUPLING 5108 026 Lubrication This Model 683 Ventilator is shipped with the appropriate grease to lubricate the piston and O rings in the cylinders and the inside of the slide valve Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Changing Piston and Cylinder This Ventilator is furnished with a 5 cc piston and cylinder installed A 30 cc cylinder and piston is also supplied for users that require larger tidal volumes Note It is extremely important when changing piston and cylinders that they be aligned to be concentric and in line with the piston shaft The cylinder is fastened to the 4 port vertical slide valve by means of two screws located between the room air inlet port and the to animal port on the front of the slide valve For ease of mounting there is an alignment bracket located below the valve body When re attaching a valve to the 683 Ventilator the cut out in the valve should sit square and parallel with the corner of the alignment bracket This should ensure that the valve and piston shaft are in line with each other The vertical slide valve is fastened to the black 1 4 aluminum sub base by four screws locat ed on the opposite side from the printed label After mounting a valve if coupling does not engage piston shaft loosen cylinder mounting screws and adjust cylinder position until piston shaft enters coupling with ease ALIGNMENT B
7. Model 683 Small Animal Ventilator User s Manual 683 Small Animal Ventilator MA1 55 0000 HARVARD APPARATUS Publication 5375 001 REV F WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manvfacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products
8. R CYCLOPROPANE ETC THESE VENTILATORS ARE NOT EXPLOSION PROOE Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Voltage Selection Fuse Replacement Voltage Selection l The 683 Ventilator is equipped with 115 230V selector feature which is incorporat ed into the power entry module located on the side of the unit see figure below The Ventilator is shipped from the factory with the voltage selector set at 115V To change voltage from 115V to 230V follow these steps a Turn off main power and disconnect power cord b Using a small flathead screwdriver pry open the fuse access door on the power entry module see figure below c Remove fuse holder by using the small flathead screwdriver d Rotate the red fuse holder 180 deg and install back into power entry module e Close small access door firmly until fully closed In power entry module window see detail below 230V should be seen f Re install power cord 2 Turn on main power switch located on the rear panel The display will now illuminate number displayed is the current strokes per minute rate Fuse Replacement Key Information l Make sure power cord is disconnected from the main power supply before servicing the fuse 2 Use only Type 3AG 1 4 x 1 1 4 0 75 amp 250 volts Type T time delay fuses Harvard Apparatus part 5153 554 or equivalent Turn off power and remove power cor
9. RACKET VALVE MOUNTING SCREWS CYLINDER MOUNTING SCREWS Publication 5375 001 REV F 5 Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Changing Piston and Cylinder cont d To remove the cylinder and piston 1 Set the Ventilator volume to maximum 2 Run Ventilator at lowest speed and stop when the helical coupling is exposed 3 Using a 0 050 Allen wrench provided disconnect the white piston from the coupling leaving the coupling on the piston shaft 4 Remove the two screws located in the front of the slide valve 5 Remove piston and cylinder 6 careful to save the O ring that is recessed in the slide valve block between the screws The O ring provides the airtight seal between the plastic cylinder and the valve block To install the piston and cylinder l Pick out the new piston and cylinder 2 Using pliers remove the piston and lubricate with the grease provided 3 Insert the lubricated piston in the cylinder so that the rear of the piston is flush with the rear of the cylinder with the 1 8 diameter coupling stud protruding 4 Fasten the cylinder to the valve block with the two screws making sure the O ring is in place Do not tighten these two screws completely but just enough to hold the cylinder in place 5 The object of alignment is to have the stud at the end of the piston be exactly in line with its mating hole in the helical coupling This insures that the
10. d from power module Use a straight blade screwdriv er to pry open the access door Remove the fuse holder and then remove the fuses from this holder as shown in the figure below Replace fuses using only 0 75A amp 250 volts Type T fuses Then replace the fuse holder E Side View Note A non operator replaceable fuse exists inside of the equipment This fuse is rated T 40mA 250V 5 x 20mm Publication 5375 001 REV F Operating the Pump 2 Connection to the Animal The vertical slide valve has four ports see figure below The top port furthest from the chassis in new orientation is the return channel from the animal which leaves the Pump at the port on the side It is at this top port that expired air can be collected or recycled middle port is the room air gas channel to the animal bottom port closest to the chassis in new orientation is the room air or gas mixture intake Note It is important that the tubes to and from the animal as short as possible The new orientation allows the animal to be as close as possible to the Ventilator for minimal dead space in the system actual connection to the animal can be either cannula with y adapter Intubation cannula with y adapter selection of trachael cannula and endotracheal tubes are listed in the Harvard Apparatus Catalog and on our website www harvardapparatus com It is important that the volum
11. e of tubing shared by inspired and expired air dead space be kept to a minimum by having the Y connector as close to the animal as possible Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Connector close to animal _ From Animal Tracheal Cannula or Endotracheal Tube To Animal AlnXGas Input CHASSIS Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Operating the Pump Ventilator Graph Tidal Volume vs Weight amp Rate for Laboratory Mammals in Resting State Apparatus dead space must be added Tidal Volume ml Ambient Temperature Selection of Rate and Volume Refer to the Ventilator Graph above to select the appropriate tidal volume and respiration rate as a function of animal body weight As a rough guide an adult rat will weigh between 200 and 500 grams with tidal volumes between 0 6 to 1 25 cm with respiration rates between 66 to 210 breaths min The average rate is 100 breaths min For most rodents the 5 cc cylinder will be adequate For larger animals it will be necessary to change the cylinder Publication 5375 001 REV F 2 Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Operating the Pump Setting Volume The volume delivered is read by observing the excursion of the O ring on the piston which is closest t
12. ing conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product is properly grounded Make Proper Connections Make sure all connections are made properly and securely Orient Equipment Properly Do not position the equipment such that it is difficult to reach the disconnecting device Observe all Terminal Ratings Review the operating manual to learn the ratings on all connections Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qual ified service personnel to perform inspection Place Product in Proper Environment Review the operating manual for guidelines for proper operating environments Observe all Warning Labels on Product Read all labels on product to ensure proper usage CAUTION Protective Ground Refer to Manual Terminal Publication 5375 001 REV F Harvard Apparatus Model 683 Small Animal Ventilator User s Manual Specifications 683 Small Animal Ventilator Specifications Rate Phase Ratio Dimensions HxLxW Weight Power Stroke Rate Volume Environmental Operating Temperature Operating Humidity Installation Category Pollution Degree Port Part Number Size ID OD Catalog No
13. ings or passive check valves and requires no adjustments Anew orientation allows for minimal system dead space and thus more precise tidal volume delivery to the animal NOTE In any correspondence please refer to the ventilator as MODEL 683 with LED RATE READ OUT The MODEL 683 is a positive volume ventilator intended to meet the respiratory requirements of small laboratory animals weighing up to 5 kg 11 Ib covering rodents through rabbits This ventilator is furnished with two interchangeable piston cylinder assemblies of 0 2 to 5 0 ml breath 5 CC and 1 5 to 30 0 ml breath 30 CC The smaller cylinder is factory installed and the larger one is supplied separately The mechanical design features a linkage that enables the piston to touch the end of the cylinder at each stroke regardless of the volume This assures that all the air gas mixture taken into the ventilator is expelled at each stroke A positive mechanical slide valve is actuated by a cam on the motor to synchronize inspiration and expiration with the motion of the piston Any NON EXPLOSIVE gas air mixture can be used with the ventilator Connection kit 73 3076 is suggested for this connection A three way slide valve is attached to the mechanism plate at the end of the cylinder and actu ated by a cam on the motor These ventilators MODELS 680 681 683 OLD or NEW STYLES are NOT TO BE USED IN AN EXPLOSIVE ATMOSPHERE OR WITH ANY EXPLOSIVE GASES SUCH AS ETHER O
14. o the valve block This piston O ring excursion is relative to the volume scale on the outside of the cylinder wall Volume change is accomplished while the Ventilator is running by turning volume knob in the desired direction Setting Rate Adjust the Rate control until the desired rate appears on the LED display Avoid running the Ventilator at rates exceeding 150 breaths min for periods in excess of 2 hours CAUTION Do not over inflate the animal A good rule of thumb is to observe the depth of respiration in the normal calm unanesthetized animal and adjust the volume to approximate this respiration depth when the animal is attached to the ventilator Use of Gas Mixture It is possible to introduce non explosive air gas mixtures into the lower room air port However the source of gas mixture is usually from a tank delivering gas is at a constant rate while the Ventilator takes in air gas intermittently Some sort of buffer expansion chamber must be provided between the tank and the Ventilator Harvard Apparatus offers a connection kit 73 3076 to depressurize the gas prior to entering the ventilator This Ventilator is not designed to take air gas inputs under pressure Creating a Positive End Expiratory Pressure PEEP To create a PEEP pressure when using the ventilator simply connect a length of tubing to the Exhaust port and submerge the other end in a column of water There are two locations in which the clip and cylinder can
15. piston moves freely within the cylinder without undue friction 6 The two screws at the front of the valve block control left to right alignment while the four screws at the rear of the slide valve control up and down alignment By adjustment of these 6 screws the proper alignment can be made Remember that the screws inter act with one another so the procedure may have to be repeated one or more times 7 When proper alignment is achieved the helical coupling should be connected If align ment is correct the coupling should slide onto the piston stud without bending of the piston rod Publication 5375 001 REV F O RING 5013 051 O RING 5013 019 VALVE BLOCK 1 Uu enuey sasn 101e Iju8A euluy 89 apoy snieieddy Publication 5375 001 REV F Notes Nh jenuejy sasn 101e Iju8A eWIUY jews 89 apoy snieieddy pjeAjeH Publication 5375 001 REV F Notes enuey sasn 101e Iju8A eWIUY Jews 289 apoy snieieddy Publication 5375 001 REV F Declaration of Conformity Application of Council Directive s 73 23 EEC 89 336 EEC Standard s to which Conformity is Declared Safety EN 61010 1 2 Edition 2001 Emissions Immunity EN 61326 1997 w A1 1998 EN 61000 4 2 1995 EN 61000 4 3 1996 EN 61000 4 4 1995 EN 61000 4 5
16. tay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse normal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or consequential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FIT NESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warranty so the above limitation may not apply to you If a defect arises within the two year warranty period promptly contact Harvard Apparatus 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our customer service department The customer is responsible for shipping charges Please allow a reasonable period of time for com pletion of repairs replacement and return If the unit is replaced the replacement unit is cov ered only
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