User's Guide to Software
Contents
1. Method Patskttskomth Edit Method Method Template Ratio Schedule v gt Create Method Copy gt Delete Method n Close 10 LabState ver 1 0 User s Guide to Software 5 3 Run a cage In the Cage Status window select Cage1 qu 1 sg A ilab E Active Cage No 1 No experiment is running 2014 09 11 16 32 40 AniLab Software and Instruments Co Ltd LabState ver 1 0 build on 2014 09 05 www anilab cn Email sales anilab cn Windows7 x86 ver6 1 Cage Status 3 Cage Data Session Variable del Run Stop Start By Video HouseLigl OFF Liquid 0 OFF TrayLigh 0 OFF 000 34 500 000 5CSRT ztsk 5SCSRT Stagell 30s ztsk 5CSRT_Stage02_20s ztsk 5CSRT_Stage03_10s ztsk 5CSRT_Stage04_5000ms ztsk 5CSRT_Stage05_2500ms ztsk 5CSRT_Stage06_1250ms ztsk 5CSRT_Stage07_1000ms ztsk n 5CSRT_Stage08_900ms ztsk 5CSRT_Stage09_800ms ztsk 5CSRT_Stagel0_700ms ztsk 5SCSRT_Stagell_600ms ztsk 5CSRT_Stagel2_500ms ztsk uditory Stage07 0 3s 4choice ztsk Auditory_Stage08_0 3s_6choice ztsk Cage l ztsk In the Cage Control window select Proj 2014 Exp1 Rat01 Rat02 and a method click the Load button Show Control Panel 0 000 0 000 X Confirm s Are you sure to load cages click YES wr LabState ver 1 0 for Operant Behavior a state not2. Main Menu LabState ver 1 0 for Operant Be Experiment View Con Chinese v English Load Default Layout Save as Default Layout Exit OMEN LabState ver 1 0 for Oper System View Animal Edit Method Edit Video Zone LabState ver 10 for Operant Be System Experiment Con v Cage Status v Cage Data v Cage Control m I O Devices Graph Video Result Data v User Defined Variables Living Monitor Other gt E LabState ver 1 0 for Operant Behavior a s System Experiment View ci ya I O Interface ee Cage Device AniLab Sof Video Device gt Sound Device Cage Statu Weight Signal Keyboard Event Network Station Event Recorder Touch Screen Setup System Chinese English Load default layout Save as default layout Experiment Animal open the setup window of animal Edit method open the setup window of Experimental Method Editor View open the view windows Cage status the running status of cages Cage Data the data when a method is running Cage Control load method and start stop a run I O Devices see sub menu Graph see submenu Video see submenu Result Data default selected data for cage User Defined Variables see submenu Living Monitor see submenu Other see submenu Configuration I O Interface configuration of i o interface card Cage Device Video Device Sound Device Weigt Signal for startle reflex Keyboard Event def
3. Rat02 Rat04 You can add project experiment and subjects Click the add Add Project e button in the project panel Input Project ID Proj pona Input a project ID such as Proj_2014 and click OK a sub director Proj_ 2014 will be created in the data directory ese Be sure to obey the rules of directories for the Project ID Experiment ID and Subject ID V 12 are not allowed for directories i u u P Select Proj 2014 and click the add button in the Add Experiment RSD experiment panel Input a experiment ID such as EXP1 and Input Experiment ID click OK er OK Cancel LabState ver 1 0 User s Guide to Software Now we add eight Rats in Proj _2014 EXP1 using the Batch create subjects panel Input 8 for How many prefix is Rat click the Add button Confirm Batch Create Subjects Pretix Ret Start from How many fe Weigh g 2500 Subject Rat Memo fo Add Experiment Subj 4 A data Project Subject J k Proj 2014 SN O ps a EXPl Sample PE nem Weight 250 0 Ranz 2500 Rat Male subject Rat gt J RatO1 Rad 250 0 Rat Male Sex Male k Rat02 Ret4 2500 Rat Male Rat03 500 Rat Male Group Ratte 250 0 Rat Male Memo A Rat04 Ret 2500 Ret Male Rats 2500 Rat Mel BASA Pies J Rat05 D Rok Ma EET Add Delete Add k R
4. LabState ver 1 0 User s Guide to Software E Active Cage No 1 No experiment is running 2014 09 11 10 15 33 AniLab Software and Instruments Co Ltd LabState ver 1 0 build on 2014 09 05 www anilab cn Email sales anilab cn Windows7 x86 ver6 1 Cage Data z Cage Control 3 Digital I O 83 Sanple Sd Load Unload B Sucrose v A Stop RPokeLiglO OFF 0 000 A HouseLigl 0 OFF 0 000 Run Stop Punp 0 OFF 0 000 0 600 E Start By Video 0 0 0 0 NPDuration ztsk OST7 TRandom S0 58 ztsk PR3 4 ztsk RGT Test ztsk RGT twice poke ztsk Test_inputtime ztsk Show Control Panel Mouse Manual Input LeftPoke 0 RightPoke 0 OFF 0 000 Directories config cage and I O interface card configuration files data all experimental data are saved here layout sub windows arrangements sound sound device configuration and wave files need license tasks experimental method tasks files temp when a cage is running the temporary data files stored here and deleted when the cage is stopped video manual started recording of video files need license zone video tracking configuration files need license Files Labstate exe the main program StateData exe a data program to view the general data for operant tasks Config ini a text file for system configuration Layout ini a text file to save th
5. task or shuttle box Cage Video show extracted image of a single cage from the video grabber Video Zone Data video tracking data User Defined Variables experimental dependent variable defined in a method editor Counter Variable Timer Variable Computed Variable Analog Variable Video Tracking Variable Living Monitor continues monitoring Cage Data Food Monitor Lickometer Monitor Bodyweight Monitor Activity Monitor Other Rotometer Hole Board Keyboard Data Touch Screen EM Video Device Configuration Sound Device Configuration Show the video image ES Load the Data Program A ilab About JExit Close LabState LabState ver 1 0 User s Guide to Software 4 System Configuration LabState is a multi function program for behavioral study Different system setup needs configuration dependant upon the kind of experiments Please refer to separate documents for detailed configurations 4 1 I O Interface Card Type DiG 5641 I Card Address o OUT P1 2 IM P3 Y Card not Exists Input Port Output Port fave Sas egenos 4 fre gens Cage ouput 4 a A 3 A h 2 3 4 1 3 y 1 la les h 4 i 4 p pne h 5 i 3 ls o h B 2 1 7 1 g o f 8 Portt Por Por Port Portt Por2 Port3 Porta Ports Ports Por v Enabled Data 0 Data 0 Manual TEST r Timebase Digital Input Deley 00 ms Ad
6. LabState version 1 0 A Graphic State Notation Program for Animal Behavioral Study User s Guide to Software AniLab Software amp Instruments Co Ltd Copyright 2006 2014 1st Edition Sept 11 2014 On line www anlab cn E mail sales anilab cn TABLE of CONENTS Di HAE o ass c AT 1 2 SOM Ware Mtalaa OA obo oie Re nR a E teste a dec oue tenia bat aa baa a bear 2 3 VIG WI COW Sot acura anaes e A D RI I tn i ue nct c 4 A System ContIgUFdtlOFi seo p yr E XO WEE XD ER ORE EY HEURE VER man ree ee awe Tae UE VE VES 6 BT VO IA 6 42 Cage DOV ICC said nak 6 4 3 Video DEVICE AAA 7 AA Sound Devi Barato head duc nanc ab ttai nato Mcd o thin en he 7 4 5 WEIEN O II II A DR Ue DR RU UA 8 M SRM eld ro aeo E A ETT TT TT TOTEM 8 Snap A te rut DU uade 9 DAL Edit experimehtaldrilidl sssasendisdese caseus qui duni costera AE 9 5 2 Edit experimental methods ni op Ebo Nbre ia dre du 10 A PP DTE S RR 11 uba ta le a 12 LabState ver 1 0 User s Guide to Software 1 Introduction LabState renamed from AniLab ver 6 54 is a graphic state notation program designed to control devices for laboratory animal behavioral research Its principle function is to control operant chambers These devices include standard digital ON OFF apparatus such as digital input devices levers nose pokes infrared d
7. P VISTA WIN7 WIN8 About State notation State notation has been increasingly utilized to describe and implement behavioral procedures since its adaptation for this purpose in 1970 The original version describes states as unique segments of an experimental procedure accompanied by specified stimulus conditions Transitions among states are triggered by inputs from the subject or by the passage of time and may be the occasion for changing stimulus conditions recording data or per forming other operations Extensive usage has suggested a number of possible improvements and the notation therefore has been expanded and modified Revisions recently incorporated in state notation increase its power as a descriptive device for effectively communicating the procedural details of reinforcement contingencies Arthur G Snapper Ronald M Kadden Geoffrey B Inglis State notation of behavioral procedures Behavior Research Methods amp Instrumentation May 1982 Volume 14 Issue 3 pp 329 342 LabState ver 1 0 User s Guide to Software 2 Software Installation LabState is a portable Windows application also called standalone application a program designed to run on a compatible computer without being installed in a way that modifies the computer s configuration information LabState does not write to the Windows registry or store its configuration files such as an INI file in the user s profile instead it stores its configuration files in th
8. Video Startle Reflex Weight Monitor Tail Suspension Activity Monitor o 0 BE pi L Holeboard Exploration Posture Monitor Delay O j sec Step Down Test Touch Screen _ Light Dark Box y KeyBoard Event Recorder PE CI RotaMeter User Task Title Comment The distributed files look like ZER 07 ABA ES Aul AIFATAState Notation ERA H 2 x config 2014 7 12 ZH HERE SO 2 LabState ver 1 0 Registered k data 2013 10 24 SHA vit L AniLab Software and Instruments Co Ltd SESEDRO C3 Uma Copyright 2006 2014 J layout 2013 10 24 SHA D44 User Task Video Tracking sound 2014 4 8 SFA SHER 7 Operant Behavior Y Digital Device J tasks 2014 6 5 8809 yit Conditioned Place Preference Analog Device q ARS Locomotion Sound Device L temp 2014 7 5 SAA MFE Conditioned Fear 7 Keyboard LR ERHEN Conflict Drinking Test OptoGenetics Light Control L video 2014 6 5 HAHI MAR Shuttle Box Food Monitor 38 itz Global Activity Licking Monitor k zone 2014 5 13 H SPE O Startle Reflex a Weight Monitor Camera cfg 2014 6 18 lt HA CFG 374 1 KE Tail Suspension Activity Monitor e s Holeboard Exploration Posture Monitor amp config ini 2014 9 11 ZH HERA 1 KE Step Down Test Touch Screen LabState exe 2014 9 5 SAA MBE 3 593 KE E cci E E Max Cages 8 Max VO Cards 1 layout ini 2014 8 27 amp HBR M RE 3 KE RotaMeter license dat 2014 6 18 E DAT xt 10 KE F StateData exe 2014 8 27 85 MBE 729 KE
9. at06 a Batch Create Subjects k Rat07 Prefix Rat J Rat08 Start from f al 4 Sample How many 8 3 k 3 Sucrose Weight g 250 0 EN ef J Rat02 Sex Male k Rat04 Memo Add Delete Batch Delete Subjects ff Close 5 2 Edit experimental method the experimental method window You can create a method or edit an Experiment Method 5CSRT_Stage01_30s ztsk 5CSRT_Stage02_20s ztsk 5CSRT_Stage03_10s ztsk ik oam Cage Cagel v Weight g 250 0 g Or the 5CSRT_Stage04_5000ms ztsk existing method using Method Template 3 emplate Ratio Schedule v gt gt Ratio Schedule Second Order Fl 15min FR10 s Conditioned Fear Vogel s Conflict Drinking 5 Choice Serial Reaction Time Startle PrePulse Inhibitoin Shuttle Box Active Avoidance CPP Video 3 Box Please refer to User s Guide to Method Editor for detailed information 5CSRT_Stage05_2500ms ztsk 5CSRT_Stage06_1250ms ztsk 5CSRT_Stage0 _1000ms ztsk 5CSRT_Stage06_900ms ztsk 5CSRT_Stage09_800ms ztsk 5CSRT_Stage10_700ms ztsk 5CSRT_Stage11_600ms ztsk 5CSRT_Stagel2_500ms ztsk Auditory_Stage0 _0 3s_4choice ztsk Auditory_Stage08_0 3s_6choice ztsk Cage 1 ztsk cage 2 ztsk Cage3FullTaskCongruent ztsk Decision Making 4 bsounds_old All4 ztsk Decision Making 4 bsounds_old ztsk FR1 ztsk JIGambTask ztsk GO ztsk GO NOGO ver 5 20 3 ztsk GO NOGO ztsk hx habitu2 ztsk hx test ztsk NoGO ztsk
10. ation program for animal behavioral study A System Experiment View Configuration About te 1 gd A ilob E Active Cage No 1 No experiment is running 2014 09 11 16 31 AniLab Software and Instruments Co Ltd LabState ver 1 0 build on 2014 09 05 www anilab cn Email sales anilab cn Windows7 x86 ver6 1 3 Cage Data Unload Session Variable PokeLigh 0 OFF 000 E PokeLi gh 0 OFF 000 El PrenatureResp oodLatency 0 000 0 000 Er Rum Stop PokeLigh 0 OFF 000 PerservativeResp 2 CorrectLatenc 0 000 0 000 CorrectTrial 0 0 0 3 InCorrectLate10 000 0 000 4 InCorrectTrial 0 0 0 0 PokeLigh 0 HouseLigzlO Liquid 0 TrayLigh 0 Start By Video 5 OmissionTrial 1 6 TotalTrials 7 FoodPerservative 5CSRT Stage02 20s ztsk 5CSRT_Stage03_10s ztsk 5CSRT_Stage04_5000ms ztsk 5CSRT_Stage05_2500ms ztsk 5CSRT_Stage06_1250ms ztsk 5CSRT_Stage07_1000ms ztsk 5CSRT_Stage08_900ms ztsk 5CSRT_Stage09_800ms ztsk ECCODT Ottana 700 94 male n Chau Cantral Panal Manca Mamial Tnm In the Cage Control panel lick Run button 11 LabState ver 1 0 User s Guide to Software 6 Data Files Data Programs StateDate exe the general data program for operant behavioral study LocDate exe data program for locomotor activity ActData exe data program for global acti
11. d Card am PCI Devices Timebase 10ms Running speed 18 us SCs I Pulse Cut Filter 0 ms NewTimebese fio Updete Real Timebase 10 047 ms 0 Access Driver x Close 4 2 Cage Device Cage un Configuration amo p OutpulCard Pot t Byte Name Low High Recorder Speedin E CO O a CO O PokeLightl 2 Pokeli 34 5000 se h h lae ha TreyLight eE m m M m jo o fo jo jo 11 n n n n Save Digital Output Pulse Event Each Pulse Event v DA Device alo fo Add Cage Delete Cage Cancel Ok LabState ver 1 0 User s Guide to Software 4 3 Video Device Camera 1 Camera 1 v Video Source HD WebCam WDM Y Stream Video Capture Video Size 640x480 v width 640 Grey Height 480 Brightness 0 lt 100 100 Contrast 10 lt 100100 En Realtek High y 58 Video File Open File gt 10 m Duration Frames Auto Run Stop Cage ASF high compession v db Cal Vol Vol a an lo 4 ja b 5 fas fo e t lo z fas jo B e bo Sound Balance 0 Left Right Speaker a laa b 100 Left Spesker 100 Right Speaker 11 TEST SOUND 1 Sound 1 a lo h 13 J52 Jo ToneGen_2 wav 14 53 fo I ToneGen_3 wav 16 55 fo Voume 90 ioo ms pl r 0 000000 White Noise Tone 500 Hz LabState ver 1 0 User s Guide to Software 4 5 Weight Signal C
12. e program s directory LabState only contains one executable file LabState exe size 3 6Mb AVR EPA ABH XR KAN LabState exe 2014 9 5 amp RRh MAR 3 593 KB When you run this file it will automatically create some directories and configuration files So do not try to run the singe file BR p ABA Sem XN config 2014 9 11 SHR xis L data 2014 9 11 SHB X44 J layout 2014 9 11 SHA xe tasks 2014 9 11 SHA UE temp 2014 9 11 ESB UE config ini 2014 9 11 SH MARE 1 KB E Labstate exe 2014 9 5 Sh MBEE 3 593 KB A license file license dat or a USB key is needed to register for selected functions AniLab will distribute this program in a fully functional copy with directories and configuration files LabState ver 1 0 Unregistered Y as ET Le pata gd A ilab E Active Cage No 1 AniLab Software and Instruments Co Ltd 3 Sime O Sta Copyright 2006 2014 AniLab Software and Instruments Co Ltd LabState ver 1 0 build on 2014 09 05 www anilab cn Email A e User Task Video Tracking Cage Status 53 Cage Control dM Operant Behavior Digital Device i e LI Conditioned Place Preference Analog Device c Load Unload Locomotion Sound Device fF Conditioned Fear Keyboard hs i J Run _Stop Conflict Drinking Test OptoGenetics Light Control Run Stop Shuttle Box Food Monitor m 3 Global Activity Licking Monitor Start By
13. e viewing windows arrangements Camera cfg video grabber or camera configuration License dat for registration Config ini can be opened using the Notepad File Cage cage box Card io card In this example Cage specified the cage configuration file is config cage box Card specified the i o interface card configuration file is config io card These configuration files can be edited in the cage configuration window and I O interface configuration window respectively Normal the configuration has been made by AniLab LabState ver 1 0 User s Guide to Software 3 View windows LabState has three kinds of windows Main window the main window is always active close it will terminate the program View window a view window can be opened and closed freely The open close status and the position of the a view window will be saved in layout ini Next time LabState will start with the same windows arrangements Setup window when a setup or configuration window is opened all operations are confined to this window it must be closed in order to make other operations LJ ae A ilab E Active Cage No 1 AniLab Software and Instruments Co Ltd LabState ver 1 0 build on 2014 09 05 No experiment is running 2014 09 11 10 20 26 www anilab cn Email sales anilab cn Windows7 x86 ver6 1 You can use the pull down menu and speed button of the main window to show the view configuration windows
14. etectors etc digital output devices lights infusion pumps etc However unlike most operant control systems LabState also supports video image analysis Animal s moving or position signal can be used in behavioral experiments such as video tracking locomotion activity conditioned place preference aversion light dark box shuttle box etc global activity immobility analysis such as conditioned fear forced swim test tail suspension test etc LabState can also control analog devices such as weight signal food intake monitoring pressure response signal acoustic startle reflex and other devices keyboard and mouse input audio output acoustic startle reflex touchscreen input used for CANTAB tasks graphical output on multiple computer monitors used for CANTAB tasks LabState is the underlying platform that runs some of the most popular behavioral tasks and task suites in use today So if you want to write your own behavioral tasks you can do so using LabState Features was specifically designed to make behavioral programming simple reliable and fast by using a graphic user interface timing of events such as lever presses is with accuracy of millisecond range runs up to or more than 16 boxes simultaneously each box runs its own task independently runs on cheap standard hardware generic PCs sound video and I O cards uses a standard operating system windows 98 ME 2000 X
15. ine key press Touchscreen setup Network Station for multi stations of touch screen Event Recorder enable disable event recording in data file LabState ver 1 0 User s Guide to Software Sub Menu I O Devices Digital I O Device Graph Analog I O Device Graph gt v Cumulative Recorder Video d Weight Graph Video d Cage Cartoon Result Data Cage Video v User Defined Variables Video Zone Data v User Defined Variables Counter Variable Timer Variable Living Monitor Computed Variable Other j Analog Variable Video Tracking Variable Living Monitor gt Living Monitor Cage Data Cds Food Monitor Ii Lickometer Monitor a v Body Weight Monitor a ME Activity Monitor RotoMeter Hole Board Cage Control Keyboard Data E Touch Screen Camnla T aa LL n Speed Button 29 Edit Experimental Animal Running schedule m I O interface Configuration e Cage Device Configuration I O Device Digital I O Device show the status of digital input output devices and manual control test for digital outputs Analog I O Device show the status of analog input output devices and manual control test for analog outputs Graph Cumulative Recorder a software emulating of the traditional cumulative recorder Wight Graph viewing analog data of load cell amplifier such as used in startle reflex Video Cage Cartoon show the position of animal in a conditioned place preference
16. onfi ici Pr Welah Soror P Rest Time s Gain mg v Detection Thred Current Device y Detection Thred fio mw Calibration Response Delay 50 E ms 4 6 Touch Screen Touch Screen Setup Zone Setup Image Setup Zone Number E c Name Axis Shape Name Hole1 Hole Square Hole Square Shape Square z Hole3 Square Left 2 Top lt Hole4 Square hole5 Square Width 100 a Height roo a Center m clBlack v Border O clWhite Width f2 S Update UP DOWN Touch Screen Window tackground Colo lii clBlack y Orig x 0 Orig Y 0 Save AS Delete LabState ver 1 0 User s Guide to Software 5 Run an Experiment There are three steps to run an experiment in a cage First you should create the experimental subject animals then create an experimental method finally select an animal and a method load into a cage and start run 5 1 Edit experimental animal LabState manages the experimental data in three levels Project Experiment Subject representing in three levels of sub directories in the data directory Click the speed button 29 to open the setup window of the experiment subjects pipet Subjects MEM Project Subject Add Delete Batch Delete Subjects In this sample there is only one project named Sample The director structure is as follow data Sample Sucrose
17. vity analysis such as tail suspension forced swim test and fear conditioning CPPDate exe data program for conditioned place preference StartleDate exe data program for acoustic startle reflex FearDate exe data program for dear conditioning UserData exe data program for user defined session variables TrackVarData exe data program for user defined video tracking variables Data File The experimental data are saved in the directory of Data Project ID Experiment ID Subject ID With a file extension name of zdat in a format of compressed text file Filename format yymmddhhmmss zdat For example a data file created in 2014 07 05 23 27 49 will be named 140705232749 zdat data x BER EKHE ASH Xv Proj 2014 k bon 140705231536 zdat 2014 7 5 SHA ZDAT xft 3 KB Ls 140705232749 zdat 2014 7 5 UUR7x ZDAT 3744 3 KB ample Sucrose Rat02 L Rat04 12
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