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1. Fixed Temp C Reset Timer 00 00 00 End PGM Main Menu Ambient Temp High flashes alternately on Fixed Temp line ABORTING Are You Sure No Yes Main Menu Dako Hybridizer Handbook the ambient temperature changes after a process is completed but before the user removes the slides and causes the instrument process set temperature to change beyond the 1 C specification for more than 2 minutes a message will appear to inform the user that the ambient temperature is high A constant beep will sound The counter will continue to count The present temperature will be displayed Hitting the Stop button will allow user to abort the process A new screen will be displayed asking user if they are sure they want to abort Hyb only PGM Name PROCESS COMPLETE Total Hyb Time End PGM Main Menu Ambient Temp High flashes alternately on PROCESS COMPLETE line Abort Screen Message ABORTING Are You Sure No Yes Main Menu programs have been created or edited the software will blank out the Create mode on the main 40 menu screen This will only allow users to edit existing programs Run a PGM Edit a PGM Present Temp C Dako Hybridizer Handbook 15 Section 3 Programming Opening and Closing the Lid The plate may be hot Use caution and check temperature on display before handling slides I2. Note If all 40 program numbers have been used the Create a PGM line in the Main Menu will no longer appear An existing program must be edited See section 5 Create a Denaturation and Hybridization Program Denat amp Hyb Turn unit on and wait for the Main Menu screen Use the arrow keys to move cursor to Create a PGM and press Enter button to accept Cursor highlights Denat amp Hyb line press Enter button to accept Hybridizer will advance to the next available program number Hybridizer allows creating a program name Cursor highlights the first name character position Use the arrow keys to move through character set and press Enter button to accept the characters All 10 character positions must be filled Press Enter button to accept blank characters For numeric characters use keypad 0 9 Character set A Z 0 9 period and blank Enter button or move arrow Cursor will advance to Denat Temp With numeric keypad enter a two digit temperature value in degrees Celsius 50 99 C Cursor advances to Denat Time With numeric keypad enter a two digit time value in minutes 0 30 Cursor advances to Hyb Temp With numeric keypad enter a two digit temperature value in degrees Celsius 30 70 C The instrument allows a temperature of 30 C or ambient temp 5 C whichever is higher for the lowest hybridization temperature For room temperature hybridization
3. ambient temp 5 C enter the two digit value 00 Cursor advances to Hyb Time With numeric keypad enter a two digit time value in hours 0 99 followed by a two digit value in minutes 0 59 Display will now show entered program values Cursor highlights Enter to Accept line Denat 82 C 5 Hyb 45 C 20 00 ENTER to Accept STOP to Abort Press Enter button to accept the program values or press Backspace button to return to previous screen to modify program values or press the Stop button to abort Note Accepting the program saves the program by its assigned program number and name Dako Hybridizer Handbook 17 Create a Hybridization Only Program Hyb Only Turn unit on and wait for the Main Menu screen Use the arrows keys to move the cursor to Create a PGM and press Enter button to accept With arrow keys move cursor to Hyb only line and press the Enter button to accept Hybridizer will advance to the next available program number PGM 02 Name Enter Temp Time Hyb 30 C 00 00 Hybridizer allows creating a program name Cursor highlights the first name character position Use the arrow keys to move through character set and press Enter button to accept the characters All 10 character positions must be filled Press Enter button to accept blank characters For numeric characters use keypad 0 9 Character set A Z 0 9 period and bl
4. 1 Unpacking and Installation azen 8 Inspect ACK AGUNG AAE 8 NS IN o ES 8 Installation Of the EOREIGZOT rss 8 E E E E 8 Section 2 System Overview a sssssz asea 10 Principle and Intended ESANA 10 SVIMIDOIS le ea aiten PAA 10 Keyboard and Main Menu Screen assa 10 Symbols and Definitions COMM EAO 11 Audible 0 oo sini aches naccsasenduyndncuaine iencweiagasncennnmonantiensacadsedenouanesasastugedncuaietensnncsepenadduabiosannsannets 12 PRUNING ETON MMCIC ALORS AAE 12 Error MGGGOOGS saso 12 Section 3 Programming 2 2 2 2 05 16 Opening and Closing Late aA 16 TOMONI O EO 16 TIN AAA 16 Create a Denaturation and Hybridization Program Denat amp EIN sura 17 Create a Hybridization Only Program Hyb ss 18 Create a Fixed Temperature Program Fixed Temp rss 19 Section 4 Operating Instructions s sss ss esec ece e eee ee eee ee eee eee 20 Opening and Closing ING 20 UR aide aA EA Run a Denat amp Hyb Program AAA 20 Run a Hyb Only Sea eela 22 RUNA ras Temp psu uaabaenastevequndaetd de noseacnanete seas 24 Abort Program in Process see eee 25 Recommended for Best 0916 26 ea eba EOC OI THT 26 SIE Te AR e a 0 26 ene enn ene ae ene ee eee 27 4 Dako Hybridizer Handbook 28 0 5 Section Closing TMe Liderren aa a aai 28 Turn Rede E netecboee gadiaane sr roe de auibb nce nm
5. Dako Handbook GY Hybridizer Handbook Document Number 0003061 Revision C July 2007 For In Vitro Diagnostic Use CE Dako Colorado Inc 4850 Innovation Drive Ft Collins CO 80525 USA 970 226 2200 Dako Hybridizer 120 V Code No 2450 Dako Hybridizer 240 V Code No 2451 Dako Hybridizer Control Strips Code No 82452 Dako Hybridizer Handbook Copyright 2007 Dako Colorado Inc All rights reserved This document may not be copied in whole or in part or reproduced in any other media without the express written permission of Dako Please note that under copyright law copying includes translation into another language Contact Dako Manufacturer Corporate Headquarters Dako Colorado Inc Dako Denmark A S 4850 Innovation Drive Produktionsvej 42 Fort Collins CO 80528 USA DK 2600 Glostrup Denmark www dako com www dako com User Resources For the latest information on Dako products and services please visit the Dako website at http www dako com Customer Support Contact your local Dako representative for customer support Visit the Dako website for the most current support information Scope This document contains basic information on the use and operation of the Hybridizer and assumes you have received basic training on the instrument Please contact your Dako representative for information not provided in this manual Dako Hybridizer Handbook 3 Table of Contents Handbook Section
6. down from the set time PGM 02 EBV Hyb in Process Hyb 55 C 01 30 Present Temp 55 C Dako Hybridizer Handbook 23 Upon program completion Hybridizer will beep five times and the display will show Process Complete Hybridization temperature will be maintained until End PGM Main Menu is accepted by pressing Enter button Before pressing Enter button remove slides for further processing If End PGM Main Menu is not accepted within the first minute of program completion hybridization time will start counting the total time at hybridization temperature PGM 02 EBV PROCESS COMPLETE Total Hyb Time 02 15 End PGM Main Menu Run a Fixed Temp Program Turn unit on and wait for the Main Menu screen Cursor highlights Run a PGM line Press Enter button to accept With the arrow keys scroll through the program numbers 1 to 40 program names If no programs have been saved advance to programming section of this handbook To accept a program number name press the Enter button Enter PGM no or Scroll arrows PGM 01 HER2 Display will confirm PGM number name and Fixed Temp Cursor highlights Run PGM line PGM 03 Appl Fixed 65 C Run PGM Main Menu Press Enter button Instrument will heat to fixed temperature Please Wait Heating to Fxd 65 C Present Temp 47 C 24 Dako Hybridizer Handbook When the fixed temp set temperature
7. mishandling or neglect DAKO MAKES NO OTHER WARRANTIES OTHER THAN THAT SPECIFIED ABOVE OF ANY KIND WHATSOEVER EXPRESS OR IMPLIED AND ALL WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE HEREBY DISCLAIMED BY DAKO AND EXCLUDED FROM THE TERMS OF SALE 32 Dako Hybridizer Handbook
8. time at hybridization temperature PGM 01 HER2 PROCESS COMPLETE Total Hyb Time 21 05 End PGM Main Menu Run a Hyb Only Program Turn unit on and wait for the Main Menu screen Cursor highlights Run a PGM line Press Enter button to accept With the arrow keys scroll through program numbers 1 to 40 program names If no programs have been saved advance to programming section of this handbook To accept press Enter button Enter PGM no or Scroll arrows PGM 01 HER2 22 Dako Hybridizer Handbook Display will confirm PGM number name and Hyb Only times and temperatures Cursor highlights the Run PGM line PGM 02 EBV Hyb 55 C 01 30 Run PGM Main Menu Press Enter button to run program The display prompts to Add Slides and Close Lid Before adding slides insert two Humidity Control Strips into the inside slide lid After slides have been added saturate strips with distilled water or equivalent approx 13 mL for dry strips and close lid Cursor highlights Start line Press Enter button to run the program To return to the Main Menu move the cursor to highlight Main Menu line and press Enter button PGM 02 EBV Add Slides Close Lid Start Main Menu Instrument will heat slides to hybridization temperature Please Wait Heating to Hyb 55 C Present Temp 45 C Once hybridization temperature is reached the time will count
9. ank Enter button or move arrow Cursor advances Hyb Temp With numeric keypad enter a two digit temperature value in degrees Celsius 30 70 C The instrument allows a temperature of 30 C or ambient temp 5 C whichever is higher for the lowest hybridization temperature For room temperature hybridization ambient temp 5 C enter the two digit value 00 Cursor advances to Hyb Time With numeric keypad enter a two digit time value in hours 0 99 followed by a two digit value in minutes 0 59 Display will now show entered program values Cursor highlights Enter to Accept line PGM 02 EBV Hyb 55 C 01 30 ENTER to Accept STOP to Abort Press Enter button to accept the program values or press Backspace button to return to previous screen to modify program values or press Stop button to abort Note Accepting the program saves the program by its assigned program number and name 18 Dako Hybridizer Handbook Create a Fixed Temperature Program Fixed Temp Use the arrows keys to move cursor Create a PGM line and press Enter button to accept With arrow keys move cursor to Fixed Temp line and press Enter button to accept Hybridizer will advance to the next available program number PGM 03 Name Enter Temp Fixed 30 C Hybridizer allows creating a program name Cursor highlights the first name character position Use the
10. arrow keys to move through character set and press Enter button to accept the characters All 10 character positions must be filled Press Enter button to accept blank characters For numeric characters use keypad 0 9 Character set A Z 0 9 period and blank Enter button or move arrow Cursor advances to Fixed Temp With numeric keypad enter a two digit temperature value in degrees Celsius 30 99 C The instrument allows a temperature of 30 C or ambient temp 5 C whichever is higher for the lowest fixed temperature For room temperature fixed ambient temp 5 C enter the two digit value 00 Display will now show entered program values Cursor highlights Enter to Accept line PGM 03 Appl Fixed 65 C ENTER to Accept STOP to Abort Press Enter button to accept the program values or press Backspace button to return to previous screen to modify program values or press Stop button to abort Note Accepting the program saves the program by its assigned program number and name Dako Hybridizer Handbook 19 Section 4 Operating Instructions Opening and Closing the Lid The plate may be hot Use caution and check temperature on display before handling slides Improper precaution can cause a burn some resistance when opening To close reverse process assure front is completely down and no obstructions prevent gasket seal from sealing on housing base Depressi
11. asket is intact and undamaged Allow unit to reach room temperature before operating Call Dako Customer Service More than 40 programs have been stored You can only edit a program Call Dako Customer Service 31 Appendix A Specifications Code Numbers Processing Time Program Number Heating Time Cooling Time Program Types gt Denaturation and Hybridization gt Hybridization only K Fixed temperature Temp Uniformity Within 1 C on the heat plate Capacity Up to 12 slides Depth 45 1 cm 17 3 4 in Width 22 8 cm 9 in Height 14 0 cm 5 1 2 in Weight 8 5 kg Environmental Indoor use Altitude up to 2000m Temperature 15 C to 40 C Maximum relative humidity 80 for temperatures up to 15 C decreasing linearly to 50 relative humidity at 40 C Main supply voltage fluctuations not to exceed 10 of the nominal voltage Transient over voltages according to installation category II Pollution degree 2 Dako Warranty Dako warrants to Buyer that the Hybridizer will be free under normal use and maintenance from defects in material and workmanship for a period of one 1 year from the date of delivery to the end user As Buyer s sole and exclusive remedy for any such defects Dako will at its option repair or replace without charge any unit which is found to be so defective Limitation of Liability Dako shall not be responsible under this warranty for Products found to be defective as a result of misuse
12. doab anauiietnaneecesatease 28 EGE ar POOF AIM EO 28 20 00 22 6 6 Section 20 EAEE 20 ame aa ent E E aca am E ated ad 30 0 bisse SIMO OUING 31 Appendix A Specifications sss esse e ccs e eee eee eee eee eee eee 2 EV 2 0 32 Bisre DION LVM AAE 32 Dako Hybridizer Handbook 5 How to use this handbook This handbook along with information contained on product labels should provide all the information needed to operate and maintain the Hybridizer Notes appear in italics to highlight information When the information requires special attention a caution symbol appears next to the italicized text Please pay close attention to the instructions that accompany the notes and symbols as well as the standard laboratory practices outlined by your facility and local regulatory agencies The table below lists all the CAUTIONS WARNINGS for the Hybridizer WARNING North American Installation Line cord should be plugged into a properly grounded AC outlet WARNING Outside of North America Inspect that the supplied Line Cord has local electrical compatibility EU Installation Use power cord with an IEC320 CEE22 female connector and male connector suitable for the power outlet to be used Cord must meet LE standards CAUTION Unplug the Hybridizer from the wall outlet before performing maintenance WARNING Do not expose Hybridizer to strong or concentrated acids bases es
13. e switch to ON position Instrument will beep to announce power has been turned on fan and heating will start and the Main Menu will be displayed when the instrument has reached the default temperature of 37 C GON O OFF Run a PGM Edit a PGM Create a PGM Present Temp 37 C Edit a Program Turn unit on and wait for the Main Menu screen Use the arrows keys to move cursor to Edit a PGM line and press Enter button to accept With the arrow keys scroll through the program numbers 1 to 40 program names If no programs have been saved advance to programming section of this manual To accept press Enter button Enter PGM no or Scroll arrows PGM 01 HER2 28 Dako Hybridizer Handbook Cursor highlights existing program type Denat 86 Hyb Hyb only or Fixed Temp Press Enter button to accept existing program type or use arrow keys to move cursor to a different program type Press Enter button to accept Select PGM Type Denat amp Hyb Hyb Only Fixed Temp Use numeric keypad to enter new values for Temperatures and or Time Procedure and limits are the same as those for creating a program Note Hybridizer allows 40 programs to be entered and stored Once all program numbers have been used an existing program must be edited PGM 04 HPV 92 C 05 37 C 16 00 Denat amp Hyb Main Menu Dako Hybridizer Handbook 29 Section 6 Mai
14. for more than four days open the lid remove strips and allow instrument to dry It is recommended to allow the instrument to dry as described above with each strip change and that the users keep the inner surface of the instrument in a clean condition For cleaning instructions advance to the cleaning section of this handbook To replace strips lift lid and remove strips Insert strips into slot positions allow fingers in lid to support strips Note Do not use paper towels in strip positions they must be folded and damage may result in lid fingers 26 Dako Hybridizer Handbook Predefined Limits Program Mode Temperature Range Room temp 30 C or Hybridization ambient 5 C whichever 0 99 hours is higher to 70 C Room temp 30 C or Fixed Temp ambient 5 C whichever 0 99 hours is higher to 99 C Dako Hybridizer Handbook section 5 Edit a Program Opening and Closing the Lid The plate may be hot Use caution and check temperature on display before handling slides Improper precaution can cause a burn Depressions located on either side of the lid allow user to lift lid into position The lid should offer some resistance when opening To close reverse process assure front is completely down and no obstructions prevent gasket seal from sealing on housing base Turn Unit On The Hybridizer main power switch is located on the rear panel Assure unit is plugged into the appropriate outlet Mov
15. g the fuse holder Remove the fuse holder and inspect type and value of fuse Replace both fuses with same type and value Insert fuse holder and push until two snaps are heard Change lid seal S2453 remove existing lid seal from the groove in the lid underside Insert new seal Be sure it is properly seated in the groove Note Decontamination before returning for service Any instrument or accessory containing accumulated blood and or other biological or chemical deposits must be cleaned prior to shipment to the manufacturer dealer for service This decontamination is required by Federal Law Title 48 and 49 of the Federal Regulations and in accordance with the Environmental Protection Agency s Regulations for Biohazard Waste Management Dako personnel cannot perform this decontamination 30 Dako Hybridizer Handbook Troubleshooting Unit does not turn on no power Poor results on slides Cannot read display Can t find Create a Program Keypad not functioning properly Dako Hybridizer Handbook Check both cord ends are plugged in Check Fuses located on rear panel next to power switch Replace with same type and value Verify selected protocol against probe manufacturer s recommendation Ensure Humidity Control Strips are in place and moist Ensure cover slip sealant was applied Ensure plate is heating Ensure lid is properly closed Ensure air intake and outlet are not obstructed Ensure lid g
16. glass coverslip over probe and sample Apply sealant along all coverslip edges Use Dako Humidity Control Strips S 2452 Slide Location Hybridizer accommodates up to 12 slides per run When prompted lift lid and load slides onto plate using slide locator for easy positioning Frosted and or label end of slides should be away from slide locator Move slides across plate until they rest against slide locator Note If slides are not resting against slide locator closing of lid may break slides Humidity Control Strips Humidity Control Strips are used to minimize evaporation of assay solution Located in the lid these strips act to prevent evaporation of probe solution from prepared slides Instructions for use After locating the slides saturate strips with distilled water or deionized water approx 13 mL for unused strips Instructions for reuse After the run has ended keep lid closed between runs to avoid drying out of the strips Do not reuse strips that have been dried out after initial saturation Resaturate the strips before starting a new run The amount of water needed to resaturate the strip depends on the ISH procedure and the time in between runs Strips should be replaced frequently as they will degrade over time and with use See package insert for Hybridizer Humidity Control Strips for further instructions Replacement strips can be purchased for use with the Hybridizer the Dako Hybridizer instrument has to stand unused
17. is reached the display prompts to Add Slides and Close Lid Before adding slides insert two Humidity Control Strips into the inside slide lid After slides have been added saturate strips with distilled water or equivalent approx 13 mL for dry strips and close lid Cursor highlights Start line Press Enter button again to run the program To return to the Main Menu move the cursor to highlight Main Menu line and press Enter button PGM 03 Appl Add Slides Close Lid Start Main Menu Display indicates present temperature of slides Timer counts elapsed time Pressing Enter button will reset timer to zero PGM 03 Appl Fixed Temp 65 C Reset Timer 01 18 10 End PGM Main Menu Use Arrow keys to move to End PGM Main Menu line and press Enter button to accept Abort Program in Process To end a program in process press STOP button three beeps will sound Use arrows to move cursor to Yes line and press Enter button to accept Program will continue to run until Yes or No has been accepted Note The Hybridizer prompts Are You Sure This measure is to prevent accidental disruption of a program in process ABORTING Are You Sure No Yes Main Menu Fan will turn on and cool to 37 C if the slide temperature is above the default temperature of 37 C Dako Hybridizer Handbook 25 Recommended for Best Results Place
18. lights Start line Press Enter button to run the program PGM 1 HER2 Add Slides Close Lid Start Main Menu To return to the Main Menu move the cursor to highlight Main Menu line and press Enter button Display indicates present temperature of the slides PGM 1 HER 2 Heating Denat 82 C 205 Present Temp 42 C Once temperature reaches denaturation set point Hybridizer will beep twice and denaturation time will count down from the set time PGM 1 HER2 Denat in Process Denat 82 C 02 28 Present Temp 82 C Dako Hybridizer Handbook 21 The Hybridizer will automatically cool to hybridization set temperature once denaturation is completed Please Wait Cooling to Hyb 45 C Present Temp 58 C Ambient Temp High flashes alternately on Present Temp line Hybridization time will count down from the set time once temperature reaches hybridization set point PGM 1 HER 2 Hyb in Process Hyb 45 C 12 48 Present Temp 45 C Upon program completion Hybridizer will beep five times and the display will show PROCESS COMPLETE Hybridization temperature will be maintained until End PGM Main Menu is accepted by pressing Enter button Before pressing Enter button remove slides for further processing If End PGM Main Menu is not accepted within the first minute of program completion hybridization time will start counting the total
19. mbient temperature is high A constant beep will sound The counter will continue to count The present temperature will be displayed Hitting the Stop button will allow the user to abort the process A new screen will be displayed asking user if they are sure they want to abort For Hyb only Please Wait Cooling to Hyb C Present Temp C Ambient Temp High flashes alternately on Present Temp line For Fixed Temp Please Wait Cooling to Fxd C Present Temp C Ambient Temp High flashes alternately on Present Temp line Abort Screen Message ABORTING Are You Sure No Yes Main Menu Dako Hybridizer Handbook 13 the ambient temperature changes during a process and causes the instrument process set temperature to change beyond the 1 C specification for more than 2 minutes a message will appear to inform the user that the ambient temperature is high A constant beep will sound The counter will continue to count The present temperature will be displayed Hitting the Stop button will allow user to abort the process A new screen will be displayed asking user if they are sure they want to abort Hyb only Fixed Temp Abort Screen Message 14 PGM Name Hyb in Process Hyb C Present Temp C Ambient Temp High flashes alternately on Hyb in Process line PGM Name
20. mproper precaution can cause a burn some resistance when opening To close reverse process assure front is completely down and no obstructions prevent gasket seal from sealing on housing base 5 Depressions located on either side of the lid allow user to lift lid into position The lid should offer Turn Unit On The Hybridizer main power switch is located on the rear panel Assure unit is plugged into the appropriate outlet Move switch to ON position Instrument will beep to announce power has been turned on fan and heating will start and the Main Menu will be displayed when the instrument has reached the default temperature of 37 C O OFF Run a PGM Edit a PGM Create a PGM Present Temp 37 C BO Overview Hybridizer is capable of storing 40 different programs Each program can be one of three program types Denaturation and Hybridization Denat amp Hyb Hybridization Only Hyb Only or Fixed Temperature Fixed Temp Select PGM Type Denat amp Hyb Hyb Only Fixed Temp Programming is simple Select program type and follow screen prompts to enter run times and set temperatures Hybridizer maintains set temperatures for duration of the processing Note At the end of the program the display will show Process Complete Temperature will be maintained and timer will continue to run until End PGM Main Menu is accepted by pressing Enter button 16 Dako Hybridizer Handbook
21. ntenance Overview Dako recommends that instrument operators perform periodic inspections and preventative maintenance on Hybridizer Contact Dako s Customer Service if the Hybridizer is not functioning properly CAUTION Unplug Hybridizer from the wall outlet before performing maintenance WARNING Do not expose Hybridizer to strong or concentrated acids bases esters aromatic or halogenated hydrocarbons ketones or strong oxidizing agents Cleaning The Hybridizer is supplied with a removable slide locating bar To remove pull locator back releasing the top from its holder the locator is spring loaded to hold it in position Lift slide locator up and remove from spring holder at bottom Set on bench top Clean the outside surfaces and switch overlay panel with a water dampened cloth and mild detergent Remove Humidity Control Strips before start of inner instrument cleaning Clean the inner surface with a mild detergent and if necessary a disinfectant wiping surfaces with a dampened cloth using 70 alcohol or 10 bleach solution Service There are no user serviceable parts except for fuses and the rubber seal in the lid Refer all other service to qualified Dako service personnel Reference the Dako Warranty for further instruction Change fuses fuses located in rear of Hybridizer between main power plug and On Off switch Unplug the Hybridizer Use a small flat screwdriver to carefully disengage the two snap locks securin
22. ons located on either side of the lid allow user to lift lid into position The lid should offer Turn Unit On The Hybridizer main power switch is located on the rear panel Assure unit is plugged into the appropriate outlet Move switch to ON position Instrument will beep to announce power has been turned on fan and heating will start and the Main Menu will be displayed when the instrument has reached the default temperature of 37 C GON O OFF Run a PGM Edit a PGM Create a PGM Present Temp 37 C Run a Denat amp Hyb Program Turn unit on and wait for the Main Menu screen Cursor highlights Run a PGM line Press Enter button to accept With the arrow keys scroll through program numbers 1 to 40 program names If no programs have been saved advance to programming section of this handbook To accept press Enter button Enter PGM no or Scroll arrows PGM 01 HER2 20 Dako Hybridizer Handbook Display will confirm PGM number name and Denat amp Hyb times and temperatures Cursor highlights Run PGM line PGM 01 HER2 82 C 05 45 C 20 00 Run PGM Main Menu Press Enter button to accept The display prompts to Add Slides and Close Lid Before adding slides insert two Humidity Control Strips into the inside slide lid After slides have been added saturate strips with distilled water or equivalent approx 13 mL for dry strips and close lid Cursor high
23. t should not be mixed with other commercial waste for disposal Dako Hybridizer Handbook Audible Indicators Single beep on all legal keystrokes Two quick beeps upon accepting a field and cursor has moved to the next field Five beeps at 0 5 second interval completion of process Audible Error Indicators Three short beeps Entering of illegal or non functioning keystrokes Low tone beep Attempt to enter a value out of acceptable range Continuous beep Instrument is not performing within acceptable range or program condition Turn off main power and restart If beep persists discontinue use and contact Dako Customer Service Error Messages Instrument cannot achieve a set temperature by heating within 10 minutes an error message appears to inform user to turn unit off and call service A constant beep will sound SYSTEM ERROR TURN UNIT OFF CALL SERVICE If the instrument cannot measure the temperature the software will automatically turn off the heating An error message will appear to inform the user to turn off the unit and call service A constant beep will sound SYSTEM ERROR TURN UNIT OFF CALL SERVICE 12 Dako Hybridizer Handbook High ambient temperature condition The instrument will attempt to achieve process set temperatures However if the cooling fan cannot achieve the set temperature within 10 minutes an error message will appear to inform the user that the a
24. temperature of 99 C The instrument is UL CUL listed and CE marked symbols and Definitions Up Move cursor up Enter character A Z for program name GEO ben Move cursor down Enter character A Z for program name cane Backspace Move cursor back to previous screen Enter numeric values for time and temperature or for program name Keyboard and Main Menu Screen 10 Dako Hybridizer Handbook symbols and Definitions cont Display Abbreviations Denat amp Hyb Denaturation amp Hybridization Denat Temp Denaturation Temperature Heating to Fixed temperature Product Reference Number Indicates the product catalog number Statement of caution warning read instruction carefully Temperature Indicates storage requirements range limitation EC Representative European Community Authorized Representative EC REP IVD For in vitro Clarifies for use as in vitro diagnostic only diagnostic use Serial Number Indicates instrument serial number code IU Consult Instructions Consult instruction manual for further explanation ei Manufactured By Indicates manufacturer of the device WEEE Correct Disposal of this Product according to Directive 2002 96 EC on Waste Electrical and Electronic Equipment WEEE applicable in the European Union and other European countries with separate collection GEA systems Contact a Dako representative for disposal of the equipment at the end of its working life This produc
25. ters aromatic or halogenated hydrocarbons ketones or strong oxidizing agents BIOHAZARD Universal Precautions should be followed on all specimens regardless of whether a specimen is known to contain an infectious agent CAUTION Risk of electric shock The instrument contains no user serviceable parts Removal of housing will expose potentially lethal voltage Refer service to qualified Dako service personal CAUTION Hot Surface The interior surface of the instrument may be HOT use caution to avoid potential burn Please use the system as intended Improper use of the Hybridizer may cause damage to the system inaccurate results or potentially nullify warranties 6 Dako Hybridizer Handbook This page is intentionally blank Dako Hybridizer Handbook Section 1 Unpacking and Installation Inspect Packaging The Hybridizer and its accessories are delivered in one carton If the instrument or accessories have suffered any damage in transport please inform your carrier immediately Note Save shipping carton and components to simplify return should service be required Verify Contents The package contains One Hybridizer code No S 2450 120V or S 2451 240V One Line Cord One Handbook 30195 One CD Handbook and Humidity Control Strips package insert 30196 One Package Humidity Control Strips code No S 2452 Installation of the Hybridizer 1 Place the Hybridizer on a level surface suitable for labora
26. tory instrumentation 2 Hybridizer has an intake fan located on bottom and fan outlet in rear assure no obstructions exist on intake or outlet 3 Position the Hybridizer away from direct sunlight and sources of heat or cold 4 Verify voltage requirements located on serial number label rear of instrument Connect Power Plug the instrument into the appropriate outlet supplying the voltage and frequency indicated on the serial number label Main power switch is located on the rear of the instrument next to the line cord power entry module WARNING North American Installation Line cord should be plugged into a properly grounded AC outlet local electrical compatibility i WARNING Outside of North America Inspect that the supplied Line Cord got EU Installation Use power cord with an IEC320 CEE22 female connector and male connector suitable for the power outlet to be used Cord must meet 6 6 standards 8 Dako Hybridizer Handbook This page is intentionally blank Dako Hybridizer Handbook section 2 System Overview Principle and Intended Use IVD For in vitro diagnostic use to co denature and hybridize probes to targets in specimens on glass microscope slides The Hybridizer is a microprocessor controlled small bench top hot plate with lid The Hybridizer allows storage of 40 programs of 3 operating options Denaturing and Hybridization Hybridization Only or Fixed Temperature Capacity for 12 slides and maximum
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