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1. lt gt MCLAB Molecular Cloning Laboratories User Manual Version 1 0 Revision Date 08 30 2013 Product name Inorganic Pyrophosphatase E coli Cat IPE 100 IPE 200 IPE 300 IPE OEM Description Inorganic pyrophosphatase PPase is ubiquitous in nature and plays an important role in energy metabolism providing a thermodynamic pull for biosynthetic reactions such as protein RNA and DNA synthesis Escheri chia coli K 12 gene ppa encoding inorganic pyrophosphatase PPase was cloned and sequenced The 5 end of the ppa MRNA was identified by primer extension mapping Source E coli strain carrying a plasmid encoding pyrophosphatase from Escherichia coli K 12 Application Role in protein RNA and DNA synthesis Catalyzing the reaction PPi H O gt 2Pi Unit Definition One unit will release 1 0 umole of inorganic orthophosphate per minute at pH 9 at 25 C Recommended Storage Conditions 20 C Reference Kornberg A 1962 On the metabolic significance of phosphorolytic and pyrophosphorolytic reactions p 251 264 In H Kasha and P Pullman ed Horizons in biochemistry Academic Press New York 1 650 872 0245 www mclab com 1
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