COOLING HEATING BLOCK User's Manual
Contents
1. 7 4 Temperature Calibration 8 5 Buzzer Setting 11 Chapter 5 Failure Analysis and troubleshooting 12 1 Wiring Diagram Mode CHB 202 13 Annex 2 Wiring Diagram Mode 202 15 Cooling amp Heating Block Chapter1 Introduction Chapter 1 Introduction The Cooling amp Heating Block is a new microcomputer based product via Peltier technology Its wide application includes sample storage storage and reaction of various kinds of enzyme DNA amplification pre denaturation of electrophoresis serum solidification and so on The Cooling Heating Block has features as below VFD display with high luminosity Temperature setting value and practical value displayed simultaneously Display setting time and practical time simultaneously Aluminum block protecting the sample from contamination Aluminum block easily replaced cleaned sterilized and suitable for different type of tubes Internal over temperature protection improving reliability Temperature bias calibration Cooling amp2. 1 Chapter 2 Specifications 2 1 Normal Operating Conditions 2 2 Transportation and Storage Conditions 2 3 Basic Parameters 2 4 3 Chapter 4 1 Structure Description 4 2 Display Panel 5 3 Key 5 Chapter 4 Operation Guide 6 1 Inspection before Power on 6 2 Single temperature amp timing setting 6 3 Multi points programs setting
3. power supply should be the default device If it is damaged the A C power line may not be repaired but it must be replaced with a new one The power supply should be free of heavy objects during the Cooling amp Heating Block s operation Keep the power supply away from areas where people gather regularly e Connect the A C Power Line While connecting or disconnecting the power line you should hold the 3 pin plug with your hand and insert the plug firmly to ensure good contact between the plug and socket Pull the plug not the cable when you need to disconnect from the mains f Design Environments The Cooling amp Heating Block should be placed in a low humidity dust free well ventilated room without caustic gas or powerful magnetic interference The Cooling amp Heating Block should not be operated in close proximity to water sources such as pools and water pipes Never cover or obstruct the openings of the Cooling amp Heating Block which are designed for ventilation and to prevent the device s interior from becoming too hot When a single device is running the shortest distance between its openings and the nearest object should be at least 50cm when two devices or more are running at the same time the shortest distance is 100 cm Do not place the device on a soft surface because that will result in adverse ventilation near the device s bottom openings Too high temperature will result in degraded performance or failure of t
4. 75V J7 4_AC28V J7 5_AC28V J7 6_AC10V J7 7_AC10V J4 1 V 4 2 1 J4 3_V J44 THERMO MODULE U2 SWITCH TRANSFORMER 1 75V 450mA 32V 250mA Pt100WZP 010 Sensor BLOCK 250V2 5A U1 AC INLET For 110V instrument U7 CPU BOARD BYQ5T3500000000 U11 U12 controler DC FAN 05 3800000000 11 1 DC12VVC 010 J1 2_DC12VGND E J2 1FAN SWITCH POWER SUPPLY J2 1 J2AFANG 2 4 J3 1_TE J4 2 VCC 75 TE J3 2_TE J4 3_HEAT ja 4 TE J4 4 15 4 J4 5 COOL jaa U6 J4 6 VCC 17 am J4 7 FAN 7428 NER THERMO SWITCH J4 8 VCC Jz U4 Bg t b us 5 9 THERMO MODULE ve SWITCH TRANSFORMER 3 4 9V 1A 6 6 6 7 1 75V 450mA 8 9 32V 250mA zi Z 8 9 BLOCK 125V2 5A U1 AC INLET J5 1 VOL J5 2 GND1 U9 J5 3_SPEEDIN J5 4_GND1 J5 5_VCC1 J5 6_VCC1 VFD J1 J3 xianshiqi J6 1_PWM J6 2_VCC J2 J6 3_HEAT J6 4_VCC J6 5 COOL J6 6 VCC J6 7 FAN J6 8 VCC J7 1 AC1 75V 47 2 17 3 1 75 17 4 AC28V 17 5 28 17 6 10 J7 7_AC10V J4 1_V J4 2 1 J4 3_V J4 4_I V Pt100WZP 010 Sensor Annex 2 Wiring Diagram Mode HB 202 For 220V instrument U3 U7 CPU BOARD TRANSFORMER BYO5T3500000000 J5 1 VOL J5 2 GND1 15 3 MOT
5. Cooling Heating Block are likely to be damaged the rated safety level to be reduced and the rated operation conditions to be affected The Bioer Co shall not be in any way responsible for the consequences resulting from operator s not observing the following requirements Note This instrument is intended for indoor use only a Grounding Considerations A C power s grounding should be reliable to safeguard against an electric shock The 3 pin plug supplied with the Cooling Heating Block s power cable is a safety device that should be matched with a suitable grounded socket Never allow the third ground pin to be floating If the 3 pin plug cannot be inserted it is recommended to ask an electrician to install an appropriate power socket b Keep Away from Electric Circuits The operator is not allowed to open the Cooling amp Heating Block randomly Changing components or adjusting certain parameters inside the device must be accomplished by the certificated professional maintenance personnel only Do not change elements while the power is still on c A C Power Considerations Before turning on the power always check if the mains voltage is within the range of required power supply and that the rating current of the power socket meets the required specification as shown in below contents Cooling amp Heating Block Important Note d A C Power Line Considerations As an accessory of the Cooling amp Heating Block the A C
6. Heating Block Chapter 2 Specifications Chapter 2 Specifications This chapter addresses the Cooling amp Heating Block s operation transportation amp storage conditions basic parameters and performance 1 Normal Operating Conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power supply 220 240V 120W 50 60Hz 100 120V 120W 50 60Hz Note Before power on please check if the above operating conditions are satisfied Pay special attention to the power cable s reliable grounding 2 Transportation and Storage Conditions Ambient temperature 20 C 55 C Relative humidity lt 80 3 Basic Parameters Model Parameters CHB 202 CHB 201 HB 202 HB 201 Standard Block A 40 x 1 5ml Optional Block B 54 x 0 5ml C 96 x 0 2ml D 24x 15mm Glass tube E 115 x 73 x 38 mm Bath block H 40 x 0 2ml G 26 x 0 5ml 24 x 1 5ml Cooling amp Heating Block Chapter 2 Specifications J 96 well ELISA plate block Dimension mm 300x200x150 LxWxH Weight kg 3 2 2 8 Note The aluminum block can be customized to the users request Please contact the distributor for your customized aluminum block 4 Performance Temperature range CHB 202 CHB 201 0 C 100 C HB 202 HB 201 Room temperature 5 C 100 C Timing Range 1 99h59min Cooling Time lt 25 minutes from 20 C to 0 C lt 50 minutes from 10
7. 0 C to 0 C for CHB 202 CHB 201 only Heating time lt 35 minutes from 20 C to 100 C HB 202 HB 201 lt 12minutes Temperature control accuracy lt 0 5 C Temperature display resolution lt 0 5 C Temperature uniformity across the block lt 0 5 C Note temperature in order As to HB 202 HB 201 please operate it from low Temperature to high Cooling amp Heating Block Chapter3 Preparations Chapter 3 Preparations This chapter introduces the Cooling amp Heating Block s mechanical structure the keyboard and each key s functions and some preparations before power on It should be read carefully before the Cooling amp Heating Block is first operated 1 Structure Description 1 Block 2 Case Main Body 8 Display Panel and Keyboard 4 Sticker Power Connector and Fuse Power Switch Cooling amp Heating Block 2 Display Panel Temperature rising indicator light Temperature keeping indicator light Temperature falling indicator light Time setting indicator light Keyboard 3 Key Functions Chapter3 Preparations Display window for current temperature Display window for setting temperature Display window for running time Display window for timing Display window for procedure Display window for temp segment in run Temperature setting key pressing 4 or W to adjust setting e a temperature value and set running temperature To set tem
8. COOLING HEATING BLOCK CHB 202 HB 202 CHB 201 HB 201 User s Manual BIOER TECHNOLOGY Co LTD The Bioer Co reserves the right to modify this manual at any time without notice Note Patent materials are included in this manual All rights reserved No part of this manual shall be photocopied reproduced or translated into other languages without written permission from the Bioer Co Please read this manual carefully before the Cooling amp Heating Block is first operated File No BYQ60060000000E USM File Version October 2009 Version 4 6 BIOER TECHNOLOGY CO LTD Cooling amp Heating Block Important Note Important Note 1 Conventions Note Because of the important information in this column please read it carefully Failure to follow the advice in this column will possibly result in damage to or the malfunctioning of the Cooling Heating Block Warning This symbol means that you should be cautious when performing this operation procedure Failure to follow the requirements in this column could possibly result in personal injury 2 Safety This is a class A equipment only suitable for use in establishments other than domestic and those directly connected to a low voltage power supply network which supplies buildings used for domestic purpose During the operation maintenance or repair of the Cooling Heating Block the following safety measures should be taken Otherwise the safeguards provided by the
9. OR J5 4 GND1 J5 5 SPEEDIN 9V 1A U9 VFD AW 2 J5 6_GND1 1 75 45 15 7 VCC1 15 8 VCC1 J3 xianshiqi J6 1 32V 250mA J6 1 PWM J6 2 J2 J6 3 HEAT J6 4 VCC J6 5 COOL J6 6 VCC J6 7 FAN J6 8 VCC 17 1 AC1 75V 775 07 2 374 7 3 1 75 WES 7 4 AC28V 57e J7 5_AC28V eZ J7 6_AC10V J7 7_AC10V U4 THERMO SWITCH J4 1 V J4 2 1 J4 3 V J4 4 1 U5 JGC4F 05D 0M HONGFEI_SSR JGC 4F 05D 0M V U8 SHIN LOAD 250VAC2A SENSOR Pt100WZP 010 CTR 4 6VDC IN SWITCH U6 U1 AC INLET HEATER UNITS For 110V instrument CPU BOARD TRANSFORMER BYOS5T3500000000 3 J5 1 VOL 4 J5 2 GND1 15 3 MOTOR U9 5 J5 4 GND1 100v 6 J5 5_SPEEDIN VERD 7 J5 6_GND1 ds 7 V6Ci 8 15 8 VCC1 9 J3 xianshiqi 41 J6 1 PWM J6 2 Ja J6 3_HEAT J6 4_VCC J6 5_COOL J6 6_VCC J6 7_FAN J6 8_VCC J7 1 AC1 75V 77 3 J7 2 AC 77 4 J7 3_AC1 75V ree 352 5620 OCA TOV C A 329 10V J7 7_AC10V U4 mE J4 1 V THERMO SWITCH 44 2 1 ja J4 3_V J4 4 1 U5 JGCAF 05D 0M HONGFEI SSR JGC 4F 05D 0M LOAD 250VAC2A CTR 4 6VDC V U8 SENSOR Pt100WZP 010 U2 SWITCH U1 AC INLET HEATER UNITS 100 120V 50 60Hz Note
10. ad contact manufacturer temperatures There is significant Cooling module Contact distributor or This change in cooling failure manufacturer phenomenon 3 speed or unavailable EN is normal for temperature below Fan failed or Contact distributor or CHB 202 ambient temperature Stopped manufacturer CHB 201 Contact distributor or Sensor failure manufacturer 4 Neither cooling nor heatin ONE Only for eating Cooling module Contact distributor or failure manufacturer 5 201 Note During the warranty period the user is not allowed to open the Cooling amp Heating Block If there is a need to open the device for an above failure Please contact the distributor or manufacturer immediately Annex1 Wiring Diagram Mode CHB 202 For 220V instrument U11 U7 U12 controler CPU BOARD BYQ5T3500000000 BYQ5T3800000000 DC FAN X J5 1_VOL J5 2 GND1 U9 J5 3 SPEEDIN X J1 4_DC12VVC J1 2_DC12VGND J5 4_GND1 J5 5 VCC1 gt 25 6 T J3 xianshiqi 45 JEZ 16 1 PWM Jes 26 2 VCC J2 PX 14 4 26 4 16 3 HEAT 6 4 J6 5 COOL J6 6 VCC J6 7 FAN J6 8 VCC U10 SWITCH POWER SUPPLY J2 1FAN J2 2 FAN J4 1 PWM 132 1 13 1 44 2 J3 2_TE 14 3 HEAT J4 4 VCC J4 5 COOL J4 6 VCC J4 7 FAN J4 8 VCC ES 7 2 4 lt lt U6 THERMO SWITCH J7 1_AC1 75V 7 2 J7 3_AC1
11. he Cooling amp Heating Block Therefore the device should be protected against any kind of heat sources like sunlight ovens or central heating equipment If the Cooling amp Heating Block is set aside for a long time it is recommended to disconnect the power line to mains and cover the device with a piece of soft cloth or plastic to prevent dust from entering Note Once one of the following events occurs you are advised to disconnect the power cable with mains and contact the distributor or ask a certificated maintenance worker for help e Liquid enters into the device The device is sprinkled or drenched The device malfunctions giving off an abnormal sound or odor The device falls onto the floor or its shell is damaged Significant changes in the device s performance g Warning Sign AN HOT SURFACE Warning There is one warning sticker read HOT SURFACE on the instrument During the program execution or in the short period after the program execution the metal part near those signs is not allowed to be touched by any part of the body for fear of the body burn Cooling amp Heating Block Important Note 3 BLOCK INSTALLMENT AND INSTRUMENT MAINTENANCE The machine has accessories as below Inner Hexagon Bolt Spring Washer and special purpose Inner Hexagon Spanner Please use special purpose Inner Hexagon Spanner to tighten four pieces Inner Hexagon Bolts and Spring Washer when fixing or changing block D
12. l be responsible for the transportation expenses of the freight to the buyer which is only applicable to domestic buyers After the warranty comes due Bioer reserves the right to charge cost price for maintenance of a defective device b Warranty Terms The above warranty is not applicable to defective devices with incorrect use abnormal operating conditions improper application and unauthorized maintenance or alteration D Cooling amp Heating Block Important Note C After sales service hotline 86 571 877 74558 Bioer makes no express warranties other than those that are described herein Any descriptions in sales promotion under specific conditions shall not create an express warranty that the goods shall conform to such description Note Once it is opened the package should be checked according to the packing list If the buyer finds any items to be missing or damaged do not hesitate to contact the distributor After the acceptance check is passed the buyer should fill out the check form and send its photocopy or fax it to the distributor Bioer establishes the archives and maintenance records with the returned form Please store the package and packing materials in a safe place in case of future device maintenance The above warranty does not extend to goods damaged as the result of cheesy package Cooling amp Heating Block Contents Contents Chapter 1 Introduction
13. nd simultaneously to enter temperature calibration interface The temperature display window will show 40 0 C automatically then the machine will start heating up to 40 C Then keep timing for 30 minutes click and TEMP in the display window will flash Press or to calibrate the temperature value e as 40 5 C according to the value showed in the IL 00 30 00 30 thermometer Press again the temperature display window will show 100 0 C automatically same as above The machine will start heating up to 100 C Keep timing for 30 minutes input Cal Lae calibration value then press to confirm After calibrating the temperature as 40 C press To quit temperature calibration interface At y 00 00 Lc this time the calibration value 40 C is unavailable After calibration for one time keep temperature points as 40 C and 100 C calibrate the difference between these two temperature points and current temperature which should be keep within 0 5 C If the difference is larger than 0 5 C please calibrate the temperature again according to the steps above till getting satisfactory value C 2 Cooling amp Heating Block 5 Buzzer Setting The machine has default function as to remind the user when it meet troubles or finish running by beeps The user can cancel this function The machine has default function to beep when users click the ke
14. oling amp Heating Block Chapter4 Operation Guide Note 1 For reliable calibration it is not permitted to calibrate the display temperature until it stays within the control limits of the target temperature for at least 30 min 2 Should adopt qualified two class standard mercurial thermometer to regulate the instrument 3 Calibration point should be the wells in the center of block to inject paraffin oil into the hole location to immerge sensor of thermometer The instrument regulates temperature through adopting linear calibration of two points The user can set the calibration point freely In the case that the second calibration point is the same as the first one the instrument will regulate the current temperature point If you adopt two point linear calibration if the calibration points are set as 40 C and 100 C other temperature points will adjust according to linearity automatically Detailed operation method as following a Press simultaneously the temperature display will display the former setting value 20 C and prompt of first calibration point as C_1 automatically b Pressing to set the first calibration temperature point as 40 C c Pressing Qe Or Qe to set the second calibration temperature point as 100 Cooling amp Heating Block Chapter4 Operation Guide Note It is recommended to set calibration points from low temperature to high temperature d Press e a
15. on t overexert and make sure that each bolt is fastened You can put the Special purpose Inner Hexagon Spanner into the bottom bath of the unit after using The conical holes over the block should be cleaned regularly with soaked cotton swab in order to ensure sufficient contact and thus good heat conduction between each conical hole and the tube inside it In case it is smeared the surface of the unit can be scrubbed with a piece of dehydrated soft wet cloth Warning When cleaning the instrument should be off Corrosive scour is not allowed to clean the surface of the instrument 4 Warranty And Service Information a Warranty The unit is warranted for a period of one month from the date of shipment from the company to be free from defects in material and workmanship Bioer Technology Co Ltd shall be obligated under this warranty to exchange the unit that proves to be defective as described herein The unit is also warranted for a period of twelve months from date of shipment from the company to be free repaired for defects in material and workmanship The Bioer s obligation under this warranty shall be limited to repair or exchange at the Bioer s option of the unit that proves to be defective as described herein The buyer is responsible for deliver to the maintenance shop designated by Bioer on all warranty claims The buyer is also responsible for the transportation expenses of the freight to the maintenance shop Bioer shal
16. perature through pressing or W continuously which is more fast and e m Timing setting key pressing or W to adjust setting timing value and set timing hours To set timing through pressing 4 or W continuously which is more fast and conveniently e To select procedure section five temperature points as 51 52 53 54 55 ng Programmable setting key Pressing Prog to make any section from S1 to S5 running continuously five points as most uA Start stop press this key to start or stop the procedure Cooling amp Heating Block Chapter4 Operation Guide Chapter 4 Operation Guide In this chapter we will explain in detail how to set single point temperature timing time and multi points programs setting and how to correct the bias between the practical temperature value and display temperature value 1 Inspection before Power on Before switching the power on please ensure that supply voltage falls within the specified limits refer to Chapter 2 the plug has been inserted into the power socket reliably the grounding of the power line is reliable fixation between block and the main is reliable Please turn off the power and contact the distributor immediately if abnormal i Warning display appears after power on Are you ready Let s begin 2 Single temperature amp timing setting a Power on for about 5 seconds display window for practical tempe
17. points as usual temperature point whenever you want to use you can adjust Multi points programs setting Pressing e can connect 51 52 51 53 51 54 51 55 52 53 52 54 52 55 53 54 53 55 54 55 among 51 52 53 54 55 into two points or multi points temp program Chapter4 Operation Guide ap CL caa 400 00 00 Aio 285 1 00 20 00 Cooling amp Heating Block Chapter4 Operation Guide Take setting program of S1 S4 as an example Pressing there appears Star the lower right corner then press L Or e to select S1 press again to confirm there appears End in the lower right corner Press IT or to select 54 then press continuously for 3 seconds after this the program strartup There s no need to press again During multi points program the lower right corner shows the initial point and terminal point Prog such as 51 4 This is the significant difference between the running interfaces of signal point program and multi point program That means single point program has no such symbol jeg 28 600 me 600 00 00 00 00 80 00 5 i 91 4 5 tad 4 Temperature Calibration The Cooling amp Heating Block s display temperature is validated before shipment from the company However you may correct the bias between the practical temperature and display temperature if there is any through the calibration key Co
18. rature shows 28 5 which is the a TIME practical temperature of the block Display window nN ri 0 00 for setting temperature shows 100 0 which is the 8 former temperature while 80 00 is the former timing time and 51 is the former temperature i section b Pressing the value of display window for setting temperature will reduce or increase from decimal digit unit digit tens digit to hundreds digit Pressing Qe or to set timing time according to the same transformation rule above 285 1000 Cooling amp Heating Block 3 Besides pressing e or Qe for three seconds continuously to amend the digit from decimal to unit from unit digit to tens digit from tens digit to hundreds digit quickly If you want to set the temperature to 40 C timing time to 60 00 pressing continuously to let the temp reach 40 C it will be confirmed and stored after 3 second Meanwhile pressing for 60 00 continuously it will be confirmed stored after 3 seconds After finishing setting section S1 pressing to run the program Pressing or 2 to set five temp point s value from S1 to S5 Pressing to select S3 then press temperature setting key and timing setting key to set the values of temperature and time according to step b You can set the values of temperature and time from S1 to S5 according to this way and you can set these five
19. ys The users can set to cancel this function Please operate in the following way a Press simultaneously the display window will read On Beep Click to shift On to OFF Press simultaneously thus the modification will be saved and function to remind trouble or finishing running is canceled Meanwhile the system will enter key buzzer setting interface The display window will read On S click to shift to OFF Press simultaneously again thus the modification is saved During setting you can press to quit the setting interface thus the modification is unavailable Chapter4 Operation Guide Cooling amp Heating Block Chapter 5 Failure Analysis and troubleshooting Chapter 5 Failure Analysis and troubleshooting In this chapter we briefly describe possible failures their reasons analysis and way of processing Failure Analysis and Processing Procedures No Phenomenon Possible Causes Processing Procedure Note Power supply Check power supply for disconnected correct connection t Display window Replace it 250V 2 5A 05 2 1 doesn t response after x20 125V 2 5A 5x20 a right fuse power on Switch failure Replace it Other Contact distributor or manufacturer Serious mismatch 2 between the practical Sensor failure or Contact distributor or and display b
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