USER MANUAL Evolve 512 Delta
Contents
1. 25 57 619 001 Rev A1 2014 Photometrics All rights reserved viii PHOTOMETRICS Evolve 512 Delta User Manual Chapter 1 Overview About This The Evolve 512 Delta User Manual is divided into five chapters It is suggested that you Manual read the entire manual before operating the camera in order to ensure proper use The chapter contents are briefly described below Note The information in these chapters applies only to the Evolve 512 Delta and is currently not applicable to any other Photometrics camera e System Installation Instructions for connecting your Evolve 512 Delta camera to your computer via the Evolve FireWire interface card e Technology Overview A basic overview of EMCCD camera technology and its benefits for low light imaging e Operating Features Discusses Evolve 512 Delta features and how to optimize them for speed sensitivity and use the different trigger modes e Troubleshooting Provides answers to camera system problems Basic Specifications Provides specifications for Evolve 512 Delta system components Precautions The CCD and other system electronics are extremely sensitive to electrostatic discharge ESD To avoid permanently damaging the system please observe the following precautions e f you are using high voltage equipment such as an arc lamp with your camera system be sure to turn the camera power on last a2. 3 niigers ireifor e 3 Software Compatibility Requiremehts crecer eerte rire Fest e t rep Ra RR LH RR kde p Rr cape enis 3 Host Computer Requiremente Jis eee tette e ede ted i ces o d eb dede ee d im edens 4 Multiple Cameras tesina An reed r aa 4 Software Installation acacia atan ERA LSD ALAE 4 Installing the FireWire Interface Card eerte nnt nan nd 4 Connecting Your Evolve 512 Delta Cartera ecrire te etre lg itt eolica 5 Technology Overview Aem 6 INTOdUCUON 5 sex E 6 EMCCD Sensor StruCtu re ci edet reet eb dete deci soe ad uae enel inb 6 EM Gain and Low Light Detection 4 arent rn duane sued iui ca ek aa E daas 6 Optimizing the EM Gall Setting uci rre rect t Rr Rc esa e usen b eR Rab ee TNR cepe reus 7 Tradeoffs with EM Cr MN 7 Operating Fealures M 8 liie A Ar ad 8 Operating Frequencies eem 8 MEC 8 TSE Bids ecce te cette cee c m Recette Eu hee Lue Le 8 SA ETUR LL ULM 9 Non Overlap uoo RT 9 Overlap Mode Simultaneous Exposure Readout sssssssssssssseeeee eene 10 Triggered Operaatio C L GNS 11 Shutter Signal Behavior 2i peces ee a pER RUE sa SER REDE RR RR Poen aAA 11 Tigger
3. 2 D a e LO 0 e gt LU USER MANUAL DC 4 PHOTOMETRICS PHOTOMETRICS Evolve 512 Delta User Manual v E Copyright 2014 Photometrics PX PHOTOMETRICS 3440 East Britannia Drive Tucson Arizona 85706 Tel 1 520 889 9933 Fax 1 520 295 0299 High Performance EMCCD amp CCD Cameras for Life Sciences All rights reserved No part of this publication may be reproduced by any means without the written permission of Photometrics Acrobat and Reader are registered trademarks of Adobe Systems Incorporated in the United States and or other countries Photometrics and PVCAM are registered trademarks of Roper Scientific Inc Intel Core is a trademark of Intel Corporation in the U S and or other countries Windows is a registered trademark of Microsoft Corporation in the United States and or other countries Other brand and product names are the trademarks or registered trademarks of their respective owners and manufacturers The information in this publication is believed to be accurate as of the publication release date However Photometrics does not assume any responsibility for any consequences including any damages resulting from the use thereof The information contained herein is subject to change without notice Revision of this publication may be issued to incorporate such change 57 619 001 Rev A1 2014 Photometrics All rights reserved ii PHOTOMETRICS Evolve 512 Delta User Manual LIMITED WA
4. Photometrics All rights reserved 2 PHOTOMETRICS Evolve 512 Delta User Manual Chapter 2 System Installation Carefully review the Precautions section in the previous chapter before performing any of the procedures outlined here Again use only an Evolve data cable and an Evolve FireWire interface card with your Evolve 512 Delta camera Using a different cable or interface card may result in permanent damage to your system Introduction Your Evolve 512 Delta camera system has the following hardware components e Camera head FireWire interface card e Data cable e Power supply with power cord e USB installation drive e Quick installation guide Evolve 512 Delta system components are linked by the FireWire data cable and controlled by your host computer system All of these hardware components should be included with your shipment Keep all the original packing materials so you can safely ship the Evolve 512 Delta system to another location or return it for service if necessary If you have any difficulty with any step of the instructions call Photometrics Customer Service Software The Evolve 512 Delta package includes the PVCAM drivers designed to allow you Compatibility to use this camera with a variety of third party imaging software To see a list of supported software visit the Photometrics website The latest version of PVCAM is recommended for use with the Evolve Delta software unless there is a preferred ve
5. for each frame to be completely read out before beginning the next exposure Note Since the software you are using may show the settings differently you should refer to the software documentation for accurate information This example is using RSImage which can be downloaded from the Photometrics website Camera Acquire Properties PM1394Cam00 x Select these parameters to operate your Evolve 512 Delta camera in Non Overlap mode eating mode Clocking mode Frame Transfer Set point 80 c Range 105 00 50 0 c Figure 3 Required Settings for Non Overlap Mode Operation 57 619 001 Rev A1 2014 Photometrics All rights reserved 9 PHOTOMETRICS Evolve 512 Delta User Manual When the camera is able to expose and read out images simultaneously it is in Overla Overlap Mode hen th bl p d read 8 ly lap Simultaneous mode Overlap mode is set by choosing Pre Sequence Clearing for the clearing mode of the camera This allows for one clear before the imaging sequence starts The E de of th his all f lear bef h ging seq h POSU following waveforms show how Overlap mode functions Readout Clears xp 1 Exp 2 Exp 3 Exposure Read out 1 Read out 2 Read out 3 Readout When using Overlap mode the frame rate is higher as compared to Non Overlap mode and provides the ability to continuously image However since you are able to expose and read out simultaneously the minimum exposure time is dependent
6. or seller with respect to the repair or replacement of any parts In no event shall Photometrics liability exceed the cost of the repair or replacement of the defective product or part This limited warranty gives you specific legal rights and you may also have other rights that may vary from state to state and from country to country Some states and countries do not allow limitations on how long an implied warranty lasts when an action may be brought or the exclusion or limitation of incidental or consequential damages so the above provisions may not apply to you When contacting us for technical support or service assistance please refer to the Photometrics factory of purchase contact your authorized Photometrics representative or reseller or visit our technical support page at www photometrics com U S Government Restricted Rights The software and documentation are provided with Restricted Rights Use duplication or disclosure by the Government is subject to restrictions as set forth in subparagraph c 1 ii of the Rights in Technical Data and Computer Software clause at DFARS 252 227 7013 or subparagraphs c 1 and 2 of the Commercial Computer Software Restricted Rights at 48 CFR 52 227 19 as applicable Contractor manufacturer is Photometrics 3440 East Britannia Drive Tucson AZ 85706 This license is effective until terminated It will terminate upon the conditions set forth above or if you fail to comply with any term he
7. EC PWR TEC GND TEC PWR TEC CONTROL 15V TEC GND 37V 37V 3 3V GND 3 3V GND 16V 57 619 001 Rev A1 2014 Photometrics All rights reserved 21 PHOTOMETRIES Evolve 512 Delta User Manual 1 O Connector Pinout The I O Input Output Status connector located on the front of the power supply provides TTL level trigger and status functions Inputs must be at least 3 15 V for a high and less than 0 9 V for a low The numbers on the I O connector diagram correspond to the numbers given to the definition of each of the pins The I O connector is a female Hirose HR10A connector An I O cable Part 37 513 001 to access Trigger Input Pin 1 Frame Readout Pin 3 Camera exposing output Pin 2 and Shutter Output Pin 4 is available from Photometrics Figure 11 Input Output Connector INPUT OUTPUT I O TABLE 1 0 INPUT DESCRIPTION 1 Trigger Input This input is internally tied high through a 4 7k resistor A rising edge of the Trigger Input signal initiates the trigger The trigger source would normally hold this input low and then drive it high to initiate the trigger 2 Camera Exposing Active high A high level on this output indicates that the Output camera is exposing integrating 3 Frame Readout Active high A high level on this output indicates that data is being transferred 4 Shutter Output TTL output for timing of external shutter driver S
8. First Mode A 11 Strobe MOGO tercer sie Pe ope e ebd perd eat de AEE E a eR S 13 Bulb MOSS scuto peste ce tp sees dnd id ecd 14 57 619 001 Rev A1 2014 Photometrics All rights reserved vii PHOTOMETRICS Evolve 512 Delta User Manual SMART Streaming iia m 15 OA PA MM LIII 15 Evolve 512 Delta Application Examples sss mem emen 17 Application Settings SUMIMANY ieri crt enean Pret cran eun SERE a RR cec deua dalt 17 Rrra n desir EESE reiea 18 System Does Not Boot Normally tpi rti clar teen RM esp n a a a 18 New Hardware Found Dialog Box Does Not Appear sssss emen 18 Images Not Displayed a ste ct oder dte r vates uo od e reped etate aii 19 Camera Running TOO Wan 2e tbe eebbbee crisp Reale DNE plis 19 PVCAM Error Message ADDEATS crocia ce sue DU Leite octo etit iens 19 Lengthy Pauses During Imaglflg minoria Euge te Te grades dass feu drap sepu dnt edid 19 Basic respec Jm 20 Evolve 512 Delta Front Side VIEWS ricos doren cc dcha oe Yep sputo b did 20 ence E 20 GED Speci Cat OMS saab 20 onis m X 21 Power connector PINOUT sitio lite Liuius eec Lie 21 I O Connector PITOBL iio ettet bete bin dadaens tenu itai Manes Sade este elite ceder 22 Power Supply Sp cifiCatiOns sacs oer dado 23 Evolve LC with Ambient Cooler sscodncicsican parcial 24 ji
9. PHOTOMETRIES Evolve 512 Delta User Manual Chapter 5 Troubleshooting If you have any difficulty while troubleshooting or do not see your camera system s symptoms listed here contact Photometrics Customer Service System Does If your operating system does not boot normally after you have installed an interface Not Boot card try installing the new card in another open slot If this does not work turn off your computer and remove the newly installed interface card Turn your computer back on If Normally your system boots normally there is probably an interrupt conflict between a previously installed expansion card and the interface card that you are installing If you need assistance resolving the interrupt conflict contact Photometrics Customer Service New Hardware If the New Hardware Found dialog box does not appear after installing a new interface card to your computer and booting Windows 7 Found Dialog Box Does Not e Check to make sure that the new interface card is inserted in an Appear expansion slot according to your computer manufacturer s instructions and that the Evolve 512 Delta system s CD ROM disc is in the host computer s CD drive e It is possible that there is a conflict between the new interface card and a previously installed expansion card With the computer s power turned off remove any previously installed expansion cards that your system does not need to function If you are unsure which cards can be saf
10. RRANTY Photometrics Photometrics us we our makes the following limited warranties These limited warranties extend to the original purchaser You you only and no other purchaser or transferee We have complete control over all warranties and may alter or terminate any or all warranties at any time we deem necessary Basic Limited Two 2 Year Warranty Photometrics warrants this product against substantial defects in materials and or workmanship for a period of up to two 2 years after shipment During this period Photometrics will repair the product or at its sole option repair or replace any defective part without charge to you You must deliver the entire product to the Photometrics factory or at our option to a factory authorized service center You are responsible for the shipping costs to return the product International customers should contact their local Photometrics authorized representative distributor for repair information and assistance or visit our technical support page at www photometrics com Limited One 1 Year Warranty on Refurbished or Discontinued Products Photometrics warrants with the exception of the CCD imaging device which carries NO WARRANTIES EXPRESS OR IMPLIED this product against defects in materials or workmanship for a period of up to one 1 year after shipment During this period Photometrics will repair or replace at its sole option any defective parts without charge to you You
11. al defects in materials and or workmanship under normal use for a period of one 1 year from shipment Photometrics does not warrant that the function of the software will meet your requirements or that operation will be uninterrupted or error free You assume responsibility for selecting the software to achieve your intended results and for the use and results obtained from the software In addition during the one 1 year limited warranty the original purchaser is entitled to receive free version upgrades Version upgrades supplied free of charge will be in the form of a download from the Internet Those customers who do not have access to the Internet may obtain the version upgrades on a CD ROM from our factory for an incidental shipping and handling charge See Item 12 in the following section of this warranty Your Responsibility for more information Owner s Manual and Troubleshooting You should read the owner s manual thoroughly before operating this product In the unlikely event that you should encounter difficulty operating this product the owner s manual should be consulted before contacting the Photometrics technical support staff or authorized service representative for assistance If you have consulted the owner s manual and the problem still persists please contact the Photometrics technical support staff or our authorized service representative See Item 12 in the following section of this warranty Your Responsibility for mor
12. bility to accurately detect small changes in signal The smallest signal that the camera is able to detect is its minimum detection ability and therefore the read noise of the camera Sensor Area Sensor Area Frame transfer Area Frame transfer Area Output or Sensor Node Readout Amplifier Normal Vdtage Clock Sendas V Serial Register Port 1 multiplication For high speed low light level applications Readout 4 Amplifier High Vatage Clock Output or Sensor Node Extended Multiplication Register Traditional CCD Evolve CCD EM Gain and When using an EMCCD to amplify the signal the factor of amplification or Low Light multiplication is called the EM Gain Using EM Gain to multiply the signal before the digitization process makes the read noise a much smaller percentage of the measurement For example Using an EM Gain of 100X and multiplying the signal by 100X before digitization is the equivalent of reducing the effect of the read noise on the measurement by the same factor 100X Detection 57 619 001 RevA1 2014 Photometrics All rights reserved 6 PHOTOMETRIES Evolve 512 Delta User Manual The use of EM Gain lowers the effective read noise of the camera and brings the minimum detection limit to the lowest possible limit This directly correlates to the ability to detect extremely low signal fluxes with the camera Optimizing the It has been experimentally determined that the read noise
13. camera system in its original containers To protect the Requirements system from excessive heat cold and moisture store at an ambient temperature between 20 C and 60 C with a relative humidity of 0 to 90 noncondensing Microscopes The camera has a standard threaded video mount and can be mounted to any Lenses and microscope that accepts a standard C mount adapter The camera also allows you to r Tripods install any lens that is compatible with a standard threaded video mount as long as its optics do not extend behind the flange of the lens The Evolve 512 Delta camera can be mounted to a tripod using the tripod mounting attachment located on the sides of the camera Repairs The Evolve 512 Delta camera system contains no user serviceable parts Repairs must be done by Photometrics Should your camera system need repair contact Photometrics Customer Service Please save the original packing materials so you can safely ship the camera system to another location or return it for repairs if necessary Note Do not open the camera Opening the Evolve 512 Delta camera voids the warranty Cleaning Clean exterior surfaces of the camera with a dry lint free cloth To remove stains contact Photometrics Customer Service To clean the camera s imaging window use only a filtered compressed air source Hand held cans are not recommended as they may spray propellant onto the window Do not touch the window 57 619 001 Rev A1 2014
14. ded by Photometrics for use with liquid cooled cameras will void any and all warranty coverage of the product Unpack the cooler and hose assembly Confirm the cooler and hoses are pre filled with yellow colored coolant Align both metal connectors on hoses into connectors on cooler Press one connector into its mate on the cooler and twist 1 8 turn to lock Repeat with the second connector Unpack camera head power supply 1394 PCle card and interface cable a NS Inspect components and set up according to quick start guide for Evolve DO NOT power on camera 8 Press each hose connector on to its mating connector on the camera listen for the click 9 Pull each connector to ensure they are locked 10 Inspect the set up to insure hose connectors are secure at cooler and camera 11 Set both the pump speed and fan speed to level 10 on the front display 12 Plug in cooler and turn on 13 Look through the clear cover on coolant reservoir to observe liquid level and confirm circulation Liquid surface will appear agitated with normal circulation 14 Turn on camera power and continue setup per quick start guide DO NOT turn on camera power without liquid circulating 15 Allow approximately 30 minutes to stabilize at target camera temperature Please note there is no temperature adjustment on the unit Power Supply Power Cable 1394 Cable Cooler Hoses PC Computer Camera Head Cooler Figur
15. deoff is the generation of Background Events or Clock Induced Charge Due to the higher voltages required during the clocking of the sensor spurious charge may be generated along the way Normally this would not be an issue due to it being easily covered up by the read noise of the sensor but when a sensor is able to detect signals as low as an electron it becomes apparent in the images very quickly By using a lower amount of EM Gain the amount of background events is minimized The Evolve platform of EMCCD cameras has been designed to keep the background events as low as possible The e2V CCD97 sensor used in the Evolve 512 Delta camera has demonstrated that over time and with extended use the performance of the EM Gain multiplication drops To maintain the expected levels of performance the EM Gain needs to be recalibrated by adjusting the voltage levels being applied to the EM Gain circuitry The Evolve 512 Delta camera offers the fastest EM Gain calibration system on the market with RapidCAL 57 619 001 Rev A1 2014 Photometrics All rights reserved 7 PHOTOMETRICS Evolve 512 Delta User Manual Chapter 4 Operating Features Introduction This section will explain the different modes of operation for the Evolve 512 Delta other features and the best modes to optimize your imaging performance Operating The Evolve 512 Delta camera has two operating frequencies or speeds 20MHz and Frequencies 10MHz The 20MHz spe
16. e 13 Liquid Cooled Evolve Setup Diagram 57 619 001 Rev A1 2014 Photometrics All rights reserved 24 PHOTOMETRICS Evolve 512 Delta User Manual INDEX A Application Settings 17 B Binning 9 Bulb Mode 14 C CCD Specifications 20 Cleaning 2 Connectors 21 E EMCCD Sensor Structure 6 EM Gain 6 Environmental Requirements 2 Evolve Liquid Cooled 24 F FireWire 4 G Gain States 8 H Host Computer 4 I O Connector Pinout 22 L Lenses 2 Low Light Detection 6 57 619 001 Rev A1 2014 Photometrics All rights reserved M Microscopes 2 Multiple Cameras 4 N Non Overlap Mode 9 O Offset Bias 8 Operating Frequencies 8 Overlap Mode 10 P Power Connector Pinout 21 Power Supply 23 PVCAM Error 19 R Rapid Cal 15 Repairs 2 S Shutter Signal Behavior 11 SMART Streaming 15 Software Installation 4 Storage Requirements 2 Strobe Mode 13 T Triggered Operation 11 Trigger First Mode 11 Tripods 2 W Warranty iii PHOTOMETRICS Evolve 512 Delta User Manual gt lt PHOTOMETRICS High Performance EMCCD amp CCD Cameras for Life Sciences www photometrics com Main Phone 1 520 889 9933 Support 1 604 530 5800 1 800 874 9789 57 619 001 Rev A1 2014 Photometrics All rights reserved 26
17. e information Your Responsibility The above Limited Warranties are subject to the following terms and conditions You must retain your bill of sale invoice and present it upon request for service and repairs or provide other proof of purchase satisfactory to Photometrics You must notify the Photometrics factory service center within thirty 30 days after you have taken delivery of a product or part that you believe to be defective With the exception of customers who claim a technical issue with the operation of the product or part all invoices must be paid in full in accordance with the terms of sale Failure to pay invoices when due may result in the interruption and or cancellation of your two 2 year limited warranty and or any other warranty expressed or implied All warranty service must be made by the Photometrics factory or at our option an authorized service center Before products or parts can be returned for service you must contact the Photometrics factory and receive a return authorization number RMA Products or parts returned for service without a return authorization evidenced by an RMA will be sent back freight collect These warranties are effective only if purchased from the Photometrics factory or one of our authorized manufacturer s representatives or distributors Unless specified in the original purchase agreement Photometrics is not responsible for installation setup or disassembly at the customer s loca
18. eady Exp 1 Exp 2 Exp 3 Exposure Read out 1 Read out 2 Read out 3 Readout Trigger First Overlap Mode 57 619 001 Rev A1 2014 Photometrics All rights reserved 12 PHOTOMETRICS Evolve 512 Delta User Manual Strobe Mode In strobe mode each frame in the sequence requires a trigger When a trigger is received the camera exposes for the exposure time set in the software If strobe mode is set to run in overlap mode all exposures except the initial one will be equal to or larger than the read out time Triggers received while trigger ready is low are ignored Process Non Overlap Trigger Ready is high ready to receive trigger Trigger is received Trigger Ready goes low Image is acquired Image is read out Trigger Ready goes high Repeat Trigger Trigger Ready Exposure Readout Strobe Triggering Non Overlap Mode Process Overlap Trigger Ready is high ready to receive trigger Trigger is received Trigger Ready goes low Image is acquired Image Read Out begins Trigger Ready goes high Repeat 57 619 001 Rev A1 2014 Photometrics All rights reserved 13 PHOTOMETRICS Evolve 512 Delta User Manual Trigger Trigger Ready Exp 1 Exp 2 Exp 3 Exposure Readout Strobe Triggering Overlap Mode Bulb Mode Process Non Overlap Trigger Ready is high ready to receive trigger Trigger is received Trigger Ready goes low Image is acquired for duration trigge
19. ed is ine most optimized delivering the highest mame rates while maintaining the lowest noise performance which delivers the best Signal to Noise imaging quality while providing the highest temporal resolution balance The 10MHz speed is included primarily for experimental scenarios where the temporal resolution is not as critical and provides better noise performance than the faster speed Gain States The factor of conversion of the detected signal to digital units or intensities reported by the camera is defined as the camera s Gain It is defined as a ratio of electrons ADU The Evolve 512 Delta has two Gain states each offering a gain conversion factor optimized for a different performance benefit Gain state 1 is designed for use with 2x2 binning and offers a higher dynamic range while Gain state 2 offers better intensity resolution and better noise performance and is the ideal imaging mode for the majority of applications It is optimized to saturate the single pixel full well of the sensor at the maximum 16 bit intensity levels The exact values of the conversion gains for each camera are provided to you on a Certificate of Performance supplied with every camera listing the gain noise and other key specifications of the camera Offset Bias CCD cameras are typically designed to produce a certain level of offset also known as bias when no light is present and the exposure time is set to zero 0 Typically the user subtracts an off
20. elta Side Panel e Verify that the power switch on the side of the camera is in the off position e Connect the power supply to the Power connector on the side of the camera e Plug the power cord into the power supply and then into a suitable wall outlet e Switch on the camera power switch on the side of camera 57 619 001 Rev A1 2014 Photometrics All rights reserved 5 PHOTOMETRIES Evolve 512 Delta User Manual Chapter 3 Technology Overview Introduction EMCCD sensors have been a relatively recent development in imaging technology appearing in the life science market in 2001 with the introduction of Cascade 650 EMCCD camera from Photometrics Offering detection capabilities of extremely low signal fluxes while being able to maintain higher temporal resolutions EMCCD cameras have solidly established themselves as the product of choice for high end low light microscopy applications EMCCD Sensor The major difference between a traditional interline CCD sensor and an EMCCD sensor is the implementation of an extended register which uses high voltages to amplify the measured signal before digitization This multiplication register is what allows for the detection of extremely low signal as the amplification of signal occurs before the Structure digitization the point where the initial uncertainty in measurement or read noise becomes apparent The read noise added during the digitization process depends on the cameras a
21. ely removed call Photometrics Customer Service Then turn your computer back on e fthe New Hardware Found dialog box still does not appear contact Photometrics Customer Service 57 619 001 Rev A1 2014 Photometrics All rights reserved 18 PHOTOMETRIES Evolve 512 Delta User Manual Images Not Displayed Camera Running Too Warm PVCAM Error Message Appears Lengthy Pauses During Imaging If no images appear e Confirm that the camera switch is set to on e Confirm that the Evolve 512 Delta camera is selected in your imaging software application e Power off the camera and the host computer and check all system connections particularly the DATA and power cables Restart e Confirm that operating system is set for at least 64k colors 16 bits e Confirm that the camera is operational by taking an image with a standard C mount lens attached to your Evolve 512 Delta Using normal room lighting place the camera on a table about 3 meters away from an object and acquire an image If the problem persists contact Photometrics Customer Service It is normal for the camera to be slightly warm to the touch while in operation However if the camera is more than slightly warm to the touch and at least one inch of space has been left around the external cooling fins for airflow switch off the camera immediately and contact Photometrics Customer Service If a PVCAM error message appears note the message s nu
22. ignal is high during Shutter Open Delay and exposure time The pin does not provide power to drive the shutter directly so an external shutter drive controller is required 5 Spare Input Reserved 6 Trigger Ready This output notifies accessory devices that the camera is ready to receive a trigger 7 TX Out Accessory Serial Transmit 8 RX IN Accessory Serial Receive 9 Power Status A high level on this output indicates that the camera power is switched on 5 V on O V off 10 GND System digital ground Any external circuitry intended to interface with the trigger control signals must reference this ground connection Table 4 Input Output Definitions 57 619 001 Rev A1 2014 Photometrics All rights reserved 22 PHOTOMETRICS Evolve 512 Delta User Manual Power Supply Specifications 57 619 001 Rev A1 2014 Photometrics Voltage Input 100 240 V 50 60 Hz Current Input 2 0A Voltage Output 3 6V 1A 17V 0 5A 37V 0 2A 15V003A 6 15V03 8A Maximum Power Output 150 W Power Supply Weight 5 lb 2 267 kg Supply Cable Length 6 ft 1 828 m Note CE certification applies to the Evolve 512 Delta only when the camera system is operated with a CE approved power supply Figure 12 Power Supply Front All rights reserved 23 PHOTOMETRICS Evolve 512 Delta User Manual Appendix Evolve LC with Ambient Cooler Warning Use of equipment not originally provi
23. lectron multiplication gain input to the camera reflects the actual gain provided by the device Settings of 1 to 1000 on the electron multiplication gain slider are mapped linearly to provide the actual multiplication gain requested by the user Due to the impact ionization method used with the electron multiplication gain register the actual gain realized by the detector with time will slowly be reduced By using the calibration routine the camera is able to re establish the electron multiplication gain slider such that it will provide the gain which is input on the gain slider This ensures the quantitative nature of the camera over time 57 619 001 Rev A1 2014 Photometrics All rights reserved 15 PHOTOMETRICS Evolve 512 Delta User Manual Even though the camera is capable of delivering large multiplication gain factors multiplication gain should only be used as needed to preserve as much dynamic range as necessary and to prolong the device s lifetime Multiplication Gain vs Slider Value 1200 1000 800 600 400 200 0 00 1 101 201 301 401 501 601 701 801 901 EM Slider Value Actual Multiplication Gain X Figure 5 DAC Setting vs Charge Multiplication Gain for Evolve 512 Delta EMCCD cameras are subject to aging of the EMCCD register as a result of its usage The Evolve 512 Delta calibration feature performs the industry s most accurate EM calibration within 3 minutes A simple t
24. mber code and contact Photometrics Customer Service If you notice lengthy pauses marked by a lot of disk activity while imaging e Close any other programs that may be running e Install more physical memory RAM in your computer system 57 619 001 Rev A1 2014 Photometrics All rights reserved 19 PHOTOMETRIES Evolve 512 Delta User Manual Chapter 6 Basic Specifications Evolve 512 Delta Front Side Views Figure 7 Evolve 512 Delta Front View Figure 8 Evolve 512 Delta Side View Camera Weight Weight 6 5 lbs 2 8 kg CCD Window UV grade fused silica Specifications Broadband MgF2 anti reflective coating on both surfaces CCD Array CCD e2v CCD97 CCD Process Back Illuminated Resolution 512x512 Pixel Size 16 um x 16 um Digitalization Readout Rate 20MHz 10 MHz Table 3 CCD Specifications 57 619 001 Rev A1 2014 Photometrics All rights reserved 20 PHOTOMETRICS Evolve 512 Delta User Manual Connectors Figure 9 Evolve 512 Delta Side Panel The following connectors are located on the side of the camera e connector Type 1 6 pin IEEE 1394a FireWire e POWER connector 25 pin Dsub connector for camera power and I O functions Pi no ut Male Plug Front View 13 1 25 14 Female Socket Front View Figure 10 Power Connector Pinout RX IO FPGA GND SHUTTER OUT SPARE IO 2 FPGA 5V EXPOSE SPARE IO 1 TX IO TRIG EOF A PWR CONTROL GND T
25. mmunicate the FireWire with your computer Interface Card After installing the interface card continue to Connecting Your Evolve 512 Delta Camera 57 619 001 Rev A1 2014 Photometrics All rights reserved 4 PHOTOMETRICS Evolve 512 Delta User Manual Connecting Your The following connectors are located on the side of the Evolve 512 Delta camera Evolve 512 Delta e DATA connector Type 1 6 pin IEEE 1394a FireWire connector for data transfer Camera e POWER connector 25 pin Dsub connector for camera power see POWER Connector Pinout section in Chapter 6 for details e Power button Turns the camera on and off The following connector is located on the power supply of the Evolve 512 Delta camera e O connector Hirose HR10A 10R 10S 10 pin connector for hardware triggering input output control signals see I O Connector Pinout Chapter 6 e To connect your Evolve 512 Delta camera e The DATA cable shown below connects your Evolve 512 Delta camera to the Evolve FireWire interface card This interface cable is identifiable by its 6 pin connectors It is designed to serve as a conduit for data Connect the straight end of the DATA cable to the Evolve FireWire interface card that you have installed in the host computer Figure 1 DATA Cable e Connect the right angled end of the DATA cable to the FireWire port indicated with a red arrow in next graphic located on the side of the camera d Figure 2 Evolve 512 D
26. must deliver the entire product to the Photometrics factory or at our option a factory authorized service center You are responsible for the shipping costs to return the product to Photometrics International customers should contact their local Photometrics representative distributor for repair information and assistance or visit our technical support page at www photometrics com Normal Wear Item Disclaimer Photometrics does not warrant certain items against defect due to normal wear and tear These items include internal and external shutters cables and connectors These items carry no warranty expressed or implied Vacuum Integrity Lifetime Warranty Photometrics warrants the vacuum integrity of all our products for a period of up to sixty 60 months from the date of shipment We warrant that the detector head will maintain the factory set operating temperature without the requirement for customer pumping Should the detector experience a Vacuum Integrity failure at anytime within sixty 60 months from the date of delivery all parts and labor needed to restore the vacuum integrity will be covered by us Responsibility for shipping charges is as described above under our Basic Limited Two 2 Year Warranty 57 619 001 Rev A1 2014 Photometrics All rights reserved iii PHOTOMETRICS Evolve 512 Delta User Manual Software Limited Warranty Photometrics warrants all of our manufactured software discs to be free from substanti
27. n Overlap e The waveform behavior is shown for the following signal Trigger In e Trigger Ready e Camera Expose e Read out Shutter Signal There are 5 shutter behavior modes that are available Behavior e Open Never Shutter is always closed e Open Pre Exposure Open before every exposure closed when not exposing e Open Pre Sequence Open before start of sequence closed at end of sequence e Open Pre Trigger Causes shutter to open before external trigger is received In non triggered mode operates as Open Pre Exposure e Open No Change Sends no signals to open or close the shutter Trigger First In trigger first mode the camera requires one trigger to begin the acquisition of a Mode stream of images Once the trigger is received the camera runs using its internal timed mode independent of any future triggers It is possible to run this triggering mode in either Overlap Mode or Non Overlap Mode 57 619 001 Rev A1 2014 Photometrics All rights reserved 11 PHOTOMETRICS Evolve 512 Delta User Manual Process Trigger Ready is High ready to receive trigger Trigger is received Trigger Ready goes low Image Sequence begins All following triggers are ignored till Trigger Ready goes high Trigger Ready goes high when sequence completes Trigger Trigger Ready NO Pb 0uNnNn A Exp 1 Exp 2 Exp 3 Exposure Read out 1 Read out 2 Read out 3 Readout Trigger First Non Overlap Mode Trigger Trigger R
28. nd power the camera off first e Never connect or disconnect any cable while the system is powered on e Although you should switch off the camera s power before disconnecting any camera system cable you do not need to power off your computer to detach the cables e Use caution when triggering high current switching devices such as an arc lamp near your system The CCD can be permanently damaged by transient voltage spikes If electrically noisy devices are present an isolated conditioned power line or dedicated isolation transformer is highly recommended e Always leave one inch of space around the camera s external cooling fins for airflow 57 619 001 Rev A1 2014 Photometrics All rights reserved 1 PHOTOMETRICS Evolve 512 Delta User Manual e Never open the camera There are no user serviceable parts inside the Evolve 512 Delta camera Opening the camera voids the warranty e Use only the FireWire interface card cables and power supply designated for this camera system Using non Evolve cables FireWire interface cards or power supplies may result in permanent damage to your system e Do not use a C mount lens with optics that extend behind the lens flange Environmental The Evolve 512 Delta camera system should be operated in a clean dry environment Requirements The camera system s ambient operating temperature is 0 C to 30 C with 80 relative humidity non condensing Storage Store the Evolve 512 Delta
29. o be Compliant to the above test standard s Tested by Jeremy Luong Reviewed by Reviewer s Name August 28 2012 Date Name Date Name Signature Other aspects Nome OOOO PLEASANTON saas sm seem The test results contained in this report refer exclusively to the product s presented for testing No liability may be assumed for models or products not referred to herein This test report may not be published or duplicated in part without permission of the testing body This test report by itself does not constitute authorization for the use of any TUV Rhemland test mark The report must not be used by the client to claim product certification approval oc TUV Rheinland of North America ine 1279 Quarry Lane Ste A Pleasanton CA 4566 Tel 925 249 9123 Fax 925 249 9124 QF09CO040 Rev 0 57 619 001 Rev A1 2014 Photometrics All rights reserved vi PHOTOMETRIES Evolve 512 Delta User Manual Table of Contents a e 1 About This Manta ricino tete rato 1 uice M 1 Environmental Requirements imc et see lp escas feline cete um da cr E blend 2 Storage REQUIFEMENTS uc cu ceci ceni Asks e cpi ced i i p Pe poet eel dee 2 Microscopes Lenses ard TripOds hit ro eret EE te denise aes cesa 2 icto 2 eru A 2 System Installa e
30. of an EMCCD camera EM Gain Setting asymptotically approaches 0 2 electrons and as such maximizing the detection capability of the camera depends on getting as close to this point as possible A good rule of thumb is to take the read noise specification for a camera available on the datasheet and multiply this number by 4X or 5X and use this value as your EM Gain setting In most situations an EM Gain of 350X or more is rarely required There are other benefits to setting the EM Gain to this value and not higher the reduction of clock induced charge which will show up in your images as single pixel events or speckles extending the performance of your EM Gain discussed below and maximizing the dynamic range Tradeoffs with Using EM Gain allows for the detection of extremely low signal levels but there are a i few tradeoffs in imaging performance that have to be made Firstly the multiplication EM Gain B M process is inherently a stochastic process and therefore there is an additional amount of uncertainty added to your measurements due to the slightly varying nature of the EM Gain multiplication performance This additional uncertainty is called the Excess Noise Factor the amount that the shot noise affects the image are increased This Excess Noise Factor has experimentally been determined to be equal to v2 or 1 4 All noise factors shot noise dark current noise except read noise are increased by this factor Another tra
31. on the time taken to complete read out As such excluding the first frame any exposure time smaller than the read out time defaults to an exposure duration equal to the read out time Camera Acquire Properties PM1394Cam00 x Temperature 80 1 C Set point 80 s Range 105 00 50 0 c Color Control CCD Parameters Apply Close Figure 4 Required Settings for Overlap Mode Operation Note In Overlap mode the minimum effective exposure time is the readout time 57 619 001 Rev A1 2014 Photometrics All rights reserved 10 PHOTOMETRIES Evolve 512 Delta User Manual Triggered The Evolve 512 Delta camera offers several methods of integration with external trigger devices such as shutters or laser illumination sources Each camera has a 10 Operation pin Hirose HR10A I O connector pinout functions are described in Chapter 5 on the power supply for trigger input out and various TTL input and output operations A special cable is available from Photometrics to access primary signals such as Trigger input Expose out Frame readout and Shutter out In the default mode the camera triggers on the rising edge of a TTL signal Evolve 512 Delta cameras support the trigger modes described in the next sections The types of triggering supported by the Evolve Delta cameras are e Trigger First Mode Overlap Non Overlap e Strobe Mode Overlap Non Overlap e Bulb Mode No
32. r signal is high Image is read out Trigger Ready goes high Repeat Trigger 2 y Ignored Trigger Ready Exp 1 Exp 2 Exp 3 Exposure 1 2 3 Readout Bulb Triggering Non Overlap Mode 57 619 001 Rev A1 2014 Photometrics All rights reserved 14 PHOTOMETRIES Evolve 512 Delta User Manual SMART f Non Overlap Overlap Mode Streaming Trigger Mode Mode Supported Supported Timed Yes Yes Trigger First Yes Yes Strobed Yes Yes Bulb Yes No Table 1 Triggering Modes The Sequenced Multiple Acquisition Real Time Streaming SMART Streaming feature for the Evolve 512 Delta provides the ability to choose a set of different exposure times for capturing images and then to stream a large number of these image sets with the smallest possible delay time in between each Normally switching between different exposure times adds extra delay for each exposure time change but SMART streaming minimizes this This feature is highly advantageous for applications that require capturing many sequences of images and thus require minimal delay to keep acquisition time manageable The availability of SMART Streaming is dependent on the imaging application and its implementation of it Please refer to the application software manual for information regarding support Rapid Cal The Evolve 512 Delta has incorporated a calibration routine which allows the camera to adjust voltages of the ADC offsets such that the e
33. reof Upon termination you agree that the software and accompanying materials and all copies thereof will be destroyed This agreement is governed by the laws of the State of Arizona You acknowledge that you have read this agreement you understand it you agree to be bound by its terms and that this is the complete and exclusive statement of the agreement between you and Photometrics regarding the software 57 619 001 Rev A1 2014 Photometrics All rights reserved v PHOTOMETRIES Evolve 512 Delta User Manual A T VRheinland Precisely Right Project 0000103220 Report Date July 25 2012 Report ap ag 1744 001 Reissue Date August 28 2012 E Photometrics Terry Puhala 3440 East Britannia Drive Tel 520 547 2718 Tucson AZ 85706 U S A tpuhala photometrics com Evolve Delta Date s Tested July 5 17 2012 TUV Rheinland of North America TUV Rheinland of North America 2305 Mission College Blvd Ste 105 1279 Quarry Lane Ste A Santa Clara CA 95054 U S A Pleasanton CA 94566 U S A Tel 925 249 9123 Tel 925 249 9123 EN 61326 1 2006 EN 5501 1 2009 A 1 2010 CISPR 11 2009 A 1 2010 FCC Part 15 Subpart B 2011 EN 61000 3 2 2006 A2 2009 EN 61000 3 3 2008 Test Location s Emissions Test Specifications EN 61326 1 2006 EN 61000 4 2 2009 EN 61000 4 3 2006 A2 2010 EN 61000 4 4 2004 A 1 2010 EN 61000 4 5 2006 EN 61000 4 6 2009 EN 61000 4 11 2004 Tes Result The above product was found t
34. rsion required by the third party software Requirements 57 619 001 Rev A1 2014 Photometrics All rights reserved 3 PHOTOMETRIES Evolve 512 Delta User Manual Host Computer The host computer PC for your Evolve 512 Delta camera must meet the following Requirements minimum requirements e Windows 7 Professional operating system e Intel Core i5 processor e 4GB of RAM or greater e Open USB port or internet access to install the driver e At least one PCI Express interface slot for the FireWire interface card Multiple PVCAM supports multiple open cameras In order to use this function it must also be Cameras supported by your imaging software If your imaging software supports multiple cameras there must be a separate Firewire interface card for each camera Software An Installation Guide appropriate to your system is included as an insert with the Installation camera system This guide provides step by step instructions for installing the camera interface software and the application software for Windows based computers Additional instructions are included for installing a FireWire interface card in your computer and capturing images The Photometrics CD ROM contains the following files e Manuals directory contains user manuals in PDF format e WinOS directory contains the files for installing on a Windows PC Installing You will be using an Evolve FireWire interface card to allow the camera to co
35. set bias from the sample image for quantitative measurement Since the offset can change based on several factors such as multiplication gain speed etc it is recommended that a fresh offset bias image be taken with the same settings as the sample image and then be subtracted from the sample image The Evolve 512 Delta has an automatically adjusting offset switch When a speed gain setting is altered the bias valve is kept as close to a pre selected offset as possible This enhances the quantitative stability of the camera 57 619 001 Rev A1 2014 Photometrics All rights reserved 8 PHOTOMETRICS Evolve 512 Delta User Manual Binning Binning combining pixels into one super pixel allows you to increase the sensitivity and frame rate On the other hand binning reduces spatial resolution The Evolve 512 Delta allows binning of 1x1 2x2 4x4 and 8x8 Non Overlap When the camera does not expose and read out images simultaneously it is in Non Mode Overlap mode Non Overlap mode is set by choosing Pre Exposure Clearing for the clearing mode of the camera This allows for a clear before each exposure The following waveforms show how Non Overlap mode functions The main benefit of Non Overlap mode is that there are no limitations imposed upon the exposure time as with Overlap mode discussed below and the set exposure time is the actual exposure time The tradeoff for this accuracy is the frame rate as you have to wait
36. the frame rate of Examples p the camera For this application select the 20MHz camera speed In addition the camera should be operated in Overlap mode This can be achieved by setting the camera clearing mode to Pre sequence If desired choose a sub region ROI and or binning to further increase the frame rate Finally on chip multiplication gain should be used at approximately 300X to boost the signal level and achieve a high signal to noise ratio Example 2 My application requires precise control of the exposure time less than the readout time and want to operate the camera in the most sensitive mode possible For better imaging characteristics select the speed to be 10 MHz Use on chip multiplication gain for increased sensitivity To achieve an exposure time less than readout time use Non Overlap mode and set the clearing mode to Pre exposure Application The table below summarizes typical Evolve 512 Delta settings for maximizing speed and sensitivity and is provided as a setup aid Actual settings will vary based on the exact Settings nature of your experiment design Summary Readout Multiplication Conversion Gain Readout Mode Speed Gain e ADU Application Super Overlap mode Resolution 20MHz 300X Gain State 2 Frame Transfer Imaging Pre Sequence Table 2 Application Type and Evolve 512 Delta Setup Parameters 57 619 001 Rev A1 2014 Photometrics All rights reserved 47
37. tion 57 619 001 Rev A1 2014 Photometrics All rights reserved iv PHOTOMETRIES Evolve 512 Delta User Manual Warranties extend only to defects in materials or workmanship as limited above and do not extend to any product or part which has e been lost or discarded by you e been damaged as a result of misuse improper installation faulty or inadequate maintenance or failure to follow instructions furnished by us e had serial numbers removed altered defaced or rendered illegible e been subjected to improper or unauthorized repair or e been damaged due to fire flood radiation or other acts of God or other contingencies beyond the control of Photometrics After the warranty period has expired you may contact the Photometrics factory or a Photometrics authorized representative for repair information and or extended warranty plans Physically damaged units or units that have been modified are not acceptable for repair in or out of warranty and will be returned as received All warranties implied by state law or non U S laws including the implied warranties of merchantability and fitness for a particular purpose are expressly limited to the duration of the limited warranties set forth above With the exception of any warranties implied by state law or non U S laws as hereby limited the forgoing warranty is exclusive and in lieu of all other warranties guarantees agreements and similar obligations of manufacturer
38. urn of the cameras nose piece closes a shutter and activates a light source which the detector uses to calibrate its EM gain This ensures that users will receive the most accurate electron multiplication and the EM gain applied matches what the user requests Simple software control will allow the user to use this feature as a manual shutter in order to block all light from the sensor in order to take dark reference frames if necessary How will Rapid Cal help Itis necessary that electron multiplication be accurate if you wish to be quantitative with the images taken by an EMCCD camera The evolve cameras Rapid Cal feature allows extremely accurate and rapid calibration of the EM Gain ensuring your data can be quantitative all of the time It is recommended that calibration be performed once a week although the requirements of calibration will vary depending on the usage of the camera Rapid Cal in action LED INDICATOR FUNCTION OFF Calibrator off FLASHING AMBER ORANGE Calibration waiting for CCD temperature lock in order to proceed FLASHING GREEN Calibrating SOLID GREEN Calibration complete RED Error Figure 6 Rapid Cal Status 57 619 001 Rev A1 2014 Photometrics All rights reserved 16 PHOTOMETRIES Evolve 512 Delta User Manual Evolve 512 Delta Example 1 Application would like to acquire a large stack of single molecule images to generate my Super Resolution data My light level is fairly low and want to optimize
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