Q-sep™ 3000 Centrifuge Operator's/Service Manual
Contents
1. 3000 Centrifuge sep Centrifuge Operator s Service Manual cat 26230 amp 26231 NOTE The programmable run time has been preset for 5 MINUTES Instructions to change this are located on page 6 Q se p m 3 0 0 0 RUN LATCH UNLOCKED p Q Q A START OPEN EMERGENCY STOP N Cat we sii 26230 www restek com 800 356 1688 TEDL EC 990005 The Q sep 3000 centrifuge complies with all requirements of UL standard 3101 20 Can CSA C22 2 No 1010 1 and Can CSA C22 2 No 1010 2 20 RESTEK Chromatography Products www restek com gt 800 356 1688 gt 814 353 1300 Table of Contents Precautions and Model Description 2 Model Description Quick Start Guide ssssonsnennsnesnen 2 pe oer maar eenmiruge oe ee ne a tronically controlled single speed horizontal laboratory Supplied Equipment 00ee 3 centrifuge with a lid safety interlock system The unit is con trolled by an electronic push button timer that is variable Available Features eeee eee 3 from 1 to 30 minutes for precise spin times and ease of use Specifications tocan coudersnincaereeneesat 3 Samples can be safely viewed through the transparent lid The imbalance detection system safely terminates a run CODO PANE ssen1ssscbasesgiense ones 4 cycle in the event that a load is severely imbalanced Entry Setup Location cceseeceecseeseeees 5 into the machine is restri2. a beeper will sound and the locking mechanism will disengage allow ing entry into the rotor chamber If it does not refer to page 11 on troubleshooting Turn the lid knob counter clockwise and open the lid Remove the samples If the machine relocks before the samples are removed press the OPEN EMERGENCY STOP button to unlock the lid for an additional fifteen 15 seconds Repeat if necessary z Q sep visit www restek com QuEChERS Tube Holder Configuration for Q sep 3000 Centrifuge The Q sep 3000 centrifuge has been designed to work with 50 15 and 2mL centrifuge tubes using the appropriate acces sories The purple tube holders are designed to hold 50mL round or conical bottom centrifuge tubes Up to 6 50mL tubes can be processed at the same time When using 50mL conical bottom centrifuge tubes place conical tube inserts into the bottom of the 50mL tube holder to prevent damage to the centrifuge tubes The red tube holders and 4 position inserts are designed to hold 15mL round or conical bottom centrifuge tubes Up to 18 15mL tubes can be processed at the same time When processing 15mL tubes one of the inner positions in the tube holder must be left empty to prevent the tubes from contacting each other when the holder moves into the horizontal position Note that either the left or right position may be left empty but this must be consistent across all holders to maintain a balanced load Purple inserts can be used to
3. Braking Time 60 seconds Timer electronic 1 to 30 minutes 1 Requirement 2 0 or 1 0 amps Current Voltage Requirement 115 or 230 10 volts Frequency 50 60 Hz Centrifuge Protection Breaker 4 amp resettable Overall Dimensions H x W x D 9 in 22 9cm x 14 5 in 36 8cm x 17 in 43 2cm Weight 39 Ibs 17 7kg visit www restek com QUEChERS sep 3 Control Panel Lid Safety Switch RUN Indicator LATCH Indicator UNLOCKED Indicator START Button OPEN EMERG STOP w restek com 800 256 1698 Button i i CYCLE j COUNTER POWER Onon gt a Power on off Button This button controls the power to the centrifuge The power is on when the button is illuminated Run Indicator Lights up when the centrifuge is in operation power is being applied to the motor Latch Indicator Lights up when the lid has been closed and latched properly Unlocked Indicator Lights up to indicate that the locking mechanism has been deactivated allowing access to the rotor chamber Start Button Begins a new run the lid must be closed Open Emergency Stop Button Allows access to the rotor chamber by disengaging the locking mechanism Entry is only permitted when the rotor is stopped Pressing this button during operation will terminate the run and unlock the lid when the rotor has come to a stop Lid Safety Switch The lid safet
4. The centrifuge will not run unless the lid is latched and the LATCH light is on 9 Turn the centrifuge on by pushing the START button 10 The RUN indicator light will illuminate 11 The unit will accelerate to its set speed 12 Listen to the sound of the centrifuge A smooth whirring sound should be heard If there are any loud or unusual sounds stop the centrifuge by pushing the OPEN EMERGENCY STOP button immediately and refer to page 11 on troubleshooting 13 While the machine is running gently try to turn the latch counter clockwise Power may be cut to the motor but you should not be able to fully turn the latch If it is possible to turn the latch and open the lid while the unit is running contact Restek Corporation or your local distributor Close and latch the lid 14 Push the OPEN EMERGENCY STOP button The RUN indicator light should go out and the motor should slow to a stop 15 The lid should remain locked until the rotor has stopped If the machine unlocks prematurely contact Restek Corporation or your local distributor Once the rotor has stopped a beeper will sound and the interlock system will become disengaged for sixty 60 seconds The UNLOCKED indicator light will be illuminated during this time 16 To gain entry into the centrifuge after this period has ended simply press the OPEN EMERGENCY STOP button The lid will unlock for fifteen 15 additional seconds R
5. latch counter clockwise to open lid 6 Place tube holders in rotor and verify they are seated properly 7 Place centrifuge tubes in the tube holders and ensure that the weight load is balanced 8 Close lid and turn latch knob clockwise to lock position LATCH indicator light will illuminate 9 Press START button to initiate operation RUN indicator light will illuminate 10 Centrifuge will spin for 5 minutes to adjust spin time see page 6 11 When the rotor has come to a full stop a beeper will sound the RUN indicator will turn off and the UNLOCKED indicator light will illuminate for 60 seconds Turn lid knob counter clockwise to open the lid and remove samples 12 If centrifuge locks again before samples are removed press OPEN EMERGENCY STOP button to disen gage locking system The UNLOCKED light will illuminate and the lid can now be opened Q sep visit www restek com QuEChERS Q sep 3000 Supplied Equipment The following items come standard with each Q sep 3000 centrifuge 1 One 1 six place horizontal rotor 2 Six 6 50mL single place tube holders 3 Six 6 50mL conical tube inserts 4 Six 6 15mL four place tube holders 5 Twenty four 24 2mL tube adapters 6 One 1 Operator s Manual 2 3 4 Di 6 Q sep 3000 Operators Uservice Manual See page 11 for replacement part catalog numbers and ordering information The rotor and rotor access
6. mode d If desired use the verify the preset run time procedure above to confirm that the adjustment is correct sep 3000 Centrifuge Operation NOTE Follow the initial setup procedure on page 5 before initial operation 1 Plug the centrifuge into an approved 115 volt cat 26230 or 230 volt cat 26231 AC 50 60 Hz outlet and press the POWER button Push the OPEN EMERGENCY STOP button verify the rotor is not running and then open the lid Place the test tube samples into the tube holders Be sure to follow the rules for balanced loads on the next page Close the lid and turn the lid knob clockwise to its complete stop position The LATCH indicator light should turn on to indicate that the latch is closed properly If the lid knob is not completely latched the LATCH indicator light will not turn on and the centrifuge will not operate 5 Turn the machine on by pushing the START button on the control panel 10 11 12 The centrifuge should begin to spin The RUN indicator light should illuminate IF A PROBLEM IS FOUND DURING A SPIN THAT REQUIRES THE CENTRIFUGE TO SHUT DOWN PRESS THE OPEN EMERGENCY STOP BUTTON The run indicator light will begin to flash when one minute remains in the cycle After the set time has elapsed the RUN indicator light will extinguish and the rotor will brake to a complete stop The UNLOCKED indicator light will illuminate
7. reach easy as the power cord is the only means of emergency disconnection Initial Setup Procedure If any problems are found during the initial setup procedure refer to the troubleshooting section on page 11 1 Plug the power cord into the rear of the centrifuge Plug the other end into an approved electrical outlet For elec trical safety the unit must always be properly grounded 2 Engage the power button on the front of the centrifuge lower left hand side The button will illuminate when it is activated 3 For safety purposes the locking system is always activated To deactivate the system in order to insert or retrieve samples press the OPEN EMERGENCY STOP button on the control panel The UNLOCKED indicator light should illuminate If it does not refer to page 11 on troubleshooting The lid will be unlocked for 15 seconds after pushing the OPEN EMERGENCY STOP button Turn the latch counter clockwise and open the lid Spin the rotor by hand check for free and level rotation If the rotor does not spin freely refer to pg 11 on troubleshooting Check the thumb screw in the middle of the rotor and make sure that it is tight Place the test tube holders inside the rotor and verify that they are seated properly oS Sy eS Close the lid Rotate the lid knob clockwise to its complete stop position The LATCH indicator light should be illumi nated If it is not make sure that the lid is latched properly
8. Restek Corporation or your distributor for further assistance Excessive noise when the machine is running e Check to see that the load is balanced e Make sure that nothing has fallen into the rotor chamber e Make sure that the rotor is installed correctly and that the center thumb screw is tight The centrifuge does not run e Check the electrical outlet e Make sure that the power button is depressed The centrifuge has power when this button is illuminated e Make sure the lid latch is turned completely clockwise to its stop position When the lid is closed properly the latch light on the control panel will illuminate e Check the circuit breaker switch at the bottom right of the machine If the switch is white the breaker has tripped Contact Restek Corporation or your authorized distributor for assistance e The printed circuit board may be damaged Contact Restek Corporation or your distributor for assistance e The Q sep 3000 centrifuge is equipped with severe out of balance detection If a severely unbalanced load is run the unit may terminate the run shortly after it begins Balance the load and restart The latch light does not come on when the lid is closed e Make sure that the unit has power e Make sure the lid latch is turned completely clockwise to its stop position The latch makes contact with a switch underneath the front top of the cabinet If this switch is not activated the light will not turn on and the
9. adapt the red tube holder for use with 2mL round or conical bottom centrifuge tubes Up to 24 2mL tubes can be processed at the same time using these inserts Different sizes of tubes can be processed simultaneously as long as the balance is properly maintained see Balanced Loads below Balanced Loads Your centrifuge must contain a balanced load in order to work properly To insure that the load is balanced keep these rules in mind when inserting test tube samples 1 Opposing tube holders must be identical 2 Opposing tube holders must be empty or loaded with an equal number of equally weighted samples 3 If an odd number of samples is to be spun use a water filled tube to balance the unpaired one NOTE The Q sep 3000 centrifuge is designed to detect serious out of balance situations and will safely terminate an unbalanced run by cutting the power to the motor If this occurs wait for the rotor to stop spinning before unlocking Balance the load before restarting the centrifuge motor 4 When loading tubes into opposing holders the holders will be balanced when the tubes are loaded diagonally from one another See the illustration below Note When loading 15mL tubes the maximum capacity is three 3 tubes per holder Keep a lower position empty to prevent tubes from contacting each other when the holder moves to the horizontal position See diagram to properly balance holders with empty positions For 15mL tubes leave one i
10. cted during operation by the safe ty interlock system The Q sep 3000 centrifuge features a Initial Setup Procedure 05 5 lighted control panel that displays the status of the machine easily viewable from a distance Finally a cycle ATONA SE U PrO CECU ea a o counter keeps a count of the total number of machine runs Operation nctics woswavesdi reese ee eess steer ses 6 for servicing and maintenance records Tube Holder Configurations 7 Balanced Loads lt accdsesersvengersensaeaes 7 Care and Preventative Service 8 PRECAUTIONS l 1 Do not attempt to unlatch the centrifuge Rotor Removal and Installation 9 i ae lid unless the Unlocked indicator light is Safety Features ee a a Re Oe RT er ee ee era 10 illuminated see pg 4 Emergency Rotor Chamber Entry 10 2 Avoid solvent contact with centrifuge lid Troubleshooting 0ce cece eeees 11 3 Insure that the weight load is balanced Replacement Parts ece cece ees 11 before running the centrifuge see pg 7 EE S LELT EEEE EE 12 Quick Start Guide 1 Choose a well ventilated area for setup 2 Plug centrifuge into properly grounded receptacle 3 Press POWER button light will illuminate 4 Press OPEN EMERGENCY STOP button to disengage locking system and verify that the rotor is not moving The UNLOCKED light will appear red It is now safe to open the lid 5 Turn
11. epeat if necessary After the centrifuge has passed this procedure it is ready for operation If you would like to make adjustments to your machine s settings please continue on to Additional Set Up Procedures visit www restek com QUEChERS Q sep Additional Setup Procedures Optional 1 Verify the Preset Run Time NOTE The centrifuge must be plugged in and the lid must be opened to access programming functions a Push and hold the START button for approximately three 3 seconds The LATCH indicator light will begin to flash indicating the centrifuge is in program mode b When you release the START button the RUN indicator light will begin to flash Each flash of the RUN indicator light represents one minute of run time If the time indicated is not desired follow the instructions below to change it 2 Change the Preset Run Time NOTE The centrifuge must be plugged in and the lid must be opened to access programming functions Q a Push and hold both the START and OPEN EMERGENCY STOP buttons for approximately three 3 seconds The LATCH indicator light will begin to flash indicating the centrifuge is in program mode Release the two buttons b Press the START button once for each minute of desired run time The RUN indicator light will flash once for each entered minute c Press the OPEN EMERGENCY STOP button to save the change and exit programming
12. es or 12 months whichever comes first Should the centrifuge require warranty or out of warranty service please contact Restek Corporation or your local distributor PATENTS amp TRADEMARKS Restek patents and trademarks are the property of Restek Corporation Other trademarks appearing in Restek literature or on its website are the property of their respective owners The Restek registered trademarks used here are registered in the United States and may also be registered in other countries T y Lit Cat GNOT1203rvA RES 2 K 2009 Restek Corporation All rights reserved Printed in the U S A Restek U S 110 Benner Circle Bellefonte PA 16823 1 814 353 1300 1 800 356 1688 fax 1 814 353 1309 www restek com Restek France phone 33 0 1 60 78 32 10 fax 33 0 1 60 78 70 90 www restek fr Restek GmbH phone 49 0 6172 2797 0 fax 49 0 6172 2797 77 www restekgmbh de Sa Restek Ireland phone 44 0 2890 814576 fax 44 0 2890 814576 e mail restekireland aol com Restek Japan phone 81 3 6459 0025 fax 81 3 6459 0025 e mail restekjapan restek com Thames Restek U K LTD phone 44 0 1494 563377 fax 44 0 1494 564990 www thamesrestek co uk
13. essary Replacement parts can be found on page 11 Motor and Electrical Maintenance The Q sep 3000 centrifuge uses a brushless DC motor with high quality electri cal components It should not need service for the life of the centrifuge Keep the Rotor Chamber Clean Every six months or whenever a tube breaks refer to the note in 3 it may be nec essary to remove the rotor and clean the rotor chamber Follow the instructions on page 9 to remove and reinstall the rotor CAUTION DURING CLEANING Once the lid has been opened unplug the power cord from the electrical outlet to elim inate the risk of electric shock during cleaning The rotor chamber rotor and accessories should be thoroughly cleaned using either methanol and or soap and water The use of halogenated solvents ketones esters or other solvents not prescribed by the manufacturer may cause dam age to the rotor and tube holders and should not be used Apply cleaning solutions with a towel or cloth Do not submerge the centrifuge in water or other cleaning solutions as this will cause damage and void the warranty Rotor and Tube Holders Itis recommended that the tube holders be replaced after 24 months of use or 25 000 cycles whichever comes first Periodically check the thumb screw in the center of the rotor to ensure that it remains tight Q sep visit www restek com QuEChERS Rotor Removal and Installation To remove the rotor 1 Unlock the centrifuge by pus
14. esume operation after a few seconds Circuit Breaker The Q sep 3000 centrifuge is protected with a 4 amp circuit breaker located at the rear of the machine and mounted to the base Any electrical short circuit will cause the breaker to cut power to the machine Imbalance Detection System In the event that an imbalanced load is spun in this centrifuge the run will be aborted A continuous two count flash of the run and latch indicator lights signals that an imbalance was detected Once the cen trifuge has safely come to a complete stop and unlocked press the OPEN button or open the lid to reset the fault Correct the imbalance before proceeding Emergency Rotor Chamber Entry In the event of power failure it may be impossible to unlock the lid by conventional means In this case entry into the rotor chamber may be made by removing the latch label and using a pen to manually disengage the locking mechanism see photo Pull the mechanism towards the control panel then unlatch and open the lid If the unit is damaged contact Restek Corporation or your distributor 10 Q sep visit www restek com QuEChERS Troubleshooting 1 Problem Solutions Problem Solutions Problem Solutions Problem Solutions Problem Solutions Problem Solutions The rotor does not spin freely e Make sure nothing has fallen into the rotor chamber e If nothing is obstructing the rotor contact
15. g knob and pawl system When the knob is rotated clockwise the pawl grips the underside of the cabinet opening and prevents the lid from open ing Amechanical stop positions the pawl and prevents it from rotating completely When rotated to the stop position the pawl makes contact with a micro switch mounted underneath the cabinet top The lid safety switch prevents the cen trifuge from operating while the lid is open An indicator light on the front of the machine will illuminate when the lid has been latched properly Lid Safety Interlock System In addition to the Lid Safety Switch the Q sep 3000 centrifuge has a true O rpm lid lock ing system The lid safety interlock system keeps the lid locked at all times even during power failure and requires that the rotor be at rest in order to unlock the lid The centrifuge will not allow entry into the rotor chamber unless the centrifuge has power and the rotor is stopped To open the lid make sure that the centrifuge is plugged in and with the rotor stopped press the OPEN EMERGENCY STOP button Note After the centrifuge has started spinning it may be possible to rotate the lid knob enough to cause the pawl to lose contact with the lid safety switch If this happens the centrifuge motor may lose power but the lid will still remain locked If the knob is accidentally moved and this situation should occur rotate the knob fully clockwise to its stop position and the centrifuge will r
16. hing the OPEN EMERGENCY STOP button then unlatch and open the lid CAUTION Unplug the centrifuge from the electrical outlet at this time to eliminate the possibility of electrical shock or other injury Remove the test tube holders Remove the thumb screw in the center of the rotor Pull up on the rotor until it is clear of the motor shaft Mm A U N Remove the rotor from the rotor chamber To install the rotor 1 Place the rotor straight down onto the motor shaft taking care to line up the slot in the bottom of the rotor with the pin in the motor shaft see picture The rotor should slide onto the shaft freely and the rotor should be level Be sure that the slot in the underside of the rotor lines up with the pin going through the motor shaft or the centrifuge may be damaged 2 Once a proper fit has been achieved replace the thumb screw and tighten The thumb screw must be properly tightened or the rotor may be damaged 3 Replace the tube holders and verify that they are seated properly with the pin in the motor shaft 4 Itis recommended that the initial setup procedures on page 5 be performed to ensure that the rotor has been installed correctly and that no damage has been done to the centrifuge during the rotor installation or chamber cleaning visit www restek com QuEChERS Q sep 9 Safety Features Lid Safety Switch The Q sep 3000 centrifuge lid is secured to the top of the cabinet by a latchin
17. machine will not run The machine does not unlock after a run has completed The lid should remain locked until the rotor has come to a complete stop and then unlock for 60 seconds If additional unlock time is needed press the OPEN EMERGENCY STOP button with the machine plugged in and the rotor stopped If the lid remains locked after this and will not unlock the electronics may have been damaged Contact Restek Corporation or your authorized distributor To access the rotor chamber follow the procedure on page 10 Emergency Rotor Chamber Entry The run time is not set to the desired length Check the preset run time by following the instructions on page 6 If the preset run time is not the desired length follow the instructions on page 6 to change it Replacement Parts for Q sep 3000 Centrifuge Part No 26232 26233 26234 26249 Description 50mL single place tube holder 2 pk 15mL 4 place tube holder 2 pk 2mL tube adapters 4 pk 50mL conical tube inserts 6 pk Ctl 26232 26233 26234 26249 visit www restek com QuEChERS Q sep 1 Sep 3000 Centrifuge Operator s Service Manual cat 26230 amp 26231 2 e RUN LATCH UNLOCKED G sep 3000 ONTO START OPEN EMERGENCY STOP IS Cat 26230 www restek com 800 356 1688 JELEC WARRANTY Restek Corporation warranties that this centrifuge is free from defects in materials and workmanship for 5 000 cycl
18. nner position empty visit www restek com QuEChERS sep Care and Preventative Service With proper care and maintenance your Q sep 3000 centrifuge will provide years of laboratory service For proper care the following steps should be taken 1 Provide Adequate Ventilation For cooling purposes the Q sep 3000 centrifuge draws in ambient air through the rear of the lid and exhausts this air out the rear of the base Do not block the rear of the centrifuge as this will not allow the machine to properly ventilate itself Always Spin Balanced Loads Make certain that opposing tube holders are filled with an equal volume sample or an equivalent weight water filled tube The Q sep 3000 centrifuge has a cushioned motor mounting design which along with its rubber feet produces excellent vibration dampening However out of balance loads may break glass test tubes or produce unsatisfactory separation results Proper load balancing will improve sample separation and extend the life of the centrifuge Refer to page 7 for additional information on balancing the load Keep the Tube Holders Clean NOTE Always follow the safety guidelines of your laboratory to properly clean up and or dispose of materials if a potentially toxic substance is spilled in or on the centrifuge If a spill occurs in the cen trifuge or tube holder thoroughly clean the rotor and both the inside and outside of the tube holder or replace the tube holder if nec
19. ories are rated for a rotation frequency of 4 130 rpm a force of 3000 xg with the 15mL tube holders Q sep 3000 Features e Swing out horizontal rotor design incorporating a unique test tube holder that produces horizontally separated samples while requiring no additional parts Fixed speed of 4 130 rpm 3 000 xg e Cool flow air design that prevents overheating of samples e Heavy gauge steel construction for safety and durability Lid safety switch that prevents the centrifuge from operating unless the lid is closed and latched e Removable rotor for easy cleaning Locking lid that allows entry into the centrifuge only after the rotor has completely stopped Brushless DC motor requires no routine maintenance cool running Clear lid for safe observation of samples and optical calibration of speed Electronically controlled timed operation variable from 1 to 30 minutes e Push button operation Indicator lights RUN green lights when power is applied to the motor LATCH yellow lights when the lid is closed and latched UNLOCKED red lights when the lock system is deactivated Cycle counter to monitor machine use e Audible indicator at the end of each run Q sep 3000 Specifications Motor Speed and Force Rating 4 130 rpm 3 000 xg Maximum Capacity with 6 Place Horizontal Rotor 6 x 50mL tubes 18 x 15mL tubes 24 x 2mL tubes Motor 1 2 H P brushless DC Nominal Acceleration Time 45 seconds Nominal
20. y switch prevents the centrifuge from operating while the lid is open If the knob is not turned completely clockwise to its stop position the centrifuge cannot operate Programming functions are accessed by first opening the lid safety switch Cycle Counter This counter increments once for each run of the centrifuge This count is used for both warranty purpos es and routine maintenance Q sep visit www restek com QuEChERS Setup Location 1 Unpack the centrifuge and verify that all of the supplied equipment is present 2 Choose a setup location which meets the following criteria a A bench top clearance height of 24 60cm is required in order to open the lid b The clearance envelope is the space around the centrifuge which is required for safety Choose a setup location which will allow for a clearance envelope of at least 30 76cm x 30 76cm with the centrifuge at the center No person or hazardous material should be permitted in the clearance envelope during operation The operator time within the envelope should be limited to the time necessary for loading unloading and centrifuge operation only c Proper ventilation is necessary to prevent the overheating of samples as well as premature failure of the cen trifuge Choose an area which will allow unencumbered air flow d No adjustment is necessary for leveling the centrifuge however the surface should be flat and level e Be sure the outlet is always within
Download Pdf Manuals
Related Search